12C<6+> ion beam radiation type breeding method for isatis tinctoria

Abstract

The invention relates to a 12C<6+> ion beam radiation type breeding method for an isatis tinctoria. The method comprises following steps of (1) selecting two kinds of parent materials according to a breeding objective, wherein one is a local germplasm with the relatively strong stress resistance, selected from local varieties, and the other is a breeding material selected from Anhui isatis tinctoria germplasm; (2) carrying out reciprocal cross on the two kinds of parent materials, then screening and reserving seeds for planting; (3) soaking the seeds in warm water, and then drying or naturally air drying, so as to obtain dried seeds; (4) soaking the dried full seeds, then air drying water on the surfaces of the seeds, irradiating a sample by a 12C<6+> ion beam, so as to obtain irradiated seeds; (5) planting the irradiated seeds in a field so as to obtain a first-generation group, carefully choosing 200 variation single plants according to the original parent; (6) continuously planting the variation single plants, harvesting, reserving seeds from second-generation single plants, continuously reserving seeds and breeding to obtain third-generations plants and fourth-generation plants; and (7) identifying and testing excellent mutation strains. The method has the high efficiency and realizes the purposes of synchronous improvement of multiple characters such as yield, quality and stress resistance within a short breeding period.

Description

technical field

[0001] The invention relates to the field of Chinese herbal medicine biotechnology breeding, in particular to Isatis indica 12 C 6+ Ion beam radiation breeding method. Background technique

[0002] As a new radiation mutagenic source, heavy ion beam has the characteristics of high energy transmission line density. In plant germplasm mutation breeding operations, its mutagenic efficiency per unit dose is 10 times higher than that of X-rays, γ-rays, electron beams, etc. , resulting in a significant increase in the proportion of double-strand breaks in DNA molecules in the nucleus, showing a more serious degree of damage, a high mutation rate, and the damage is not easy to repair, and the mutant is stable. These characteristics have great advantages in mutation breeding, which can achieve the purpose of mutagenesis with a small dosage, and have high mutation rate, good stability of mutants and short breeding cycle. The heavy ion parameters are diverse, which...

Examples

Embodiment 1

[0018] Example 1 Woad 12 C 6+ Ion beam radiation breeding method, comprising the following steps:

[0019] (1) Two types of parental materials were selected according to the breeding objectives: one is local germplasm with strong stress resistance selected from local varieties, and used as the source of drought-tolerant genes for hybrid offspring in breeding; The selected breeding line has excellent growth speed and regeneration, and has excellent production performance under the condition of sufficient water and fertilizer.

[0020] (2) Carry out reciprocal crossing of the two types of parental materials in step (1) according to conventional methods, and screen and reserve seeds according to conventional methods in combination with yield, disease resistance, cold resistance and active ingredient content from the hybrid lines produced.

[0021] (3) Soak the seeds obtained in step (2) with warm water at 30°C for 36 hours, then dry or air-dry naturally to obtain dried se...

Embodiment 2

[0026] Example 2 Woad 12 C 6+ Ion beam radiation breeding method, comprising the following steps:

[0027] (1) Two types of parental materials were selected according to the breeding objectives: one is local germplasm with strong stress resistance selected from local varieties, and used as the source of drought-tolerant genes for hybrid offspring in breeding; The selected breeding line has excellent growth speed and regeneration, and has excellent production performance under the condition of sufficient water and fertilizer.

[0028] (2) Carry out reciprocal crossing of the two types of parental materials in step (1) according to conventional methods, and screen and reserve seeds according to conventional methods in combination with yield, disease resistance, cold resistance and active ingredient content from the hybrid lines produced.

[0029] (3) Soak the seeds obtained in step (2) with warm water at 50° C. for 12 hours, then dry or air-dry naturally to obtain dried ...

Embodiment 3

[0034] Example 3 Woad 12 C 6+ Ion beam radiation breeding method, comprising the following steps:

[0035](1) Two types of parental materials were selected according to the breeding objectives: one is local germplasm with strong stress resistance selected from local varieties, and used as the source of drought-tolerant genes for hybrid offspring in breeding; The selected breeding line has excellent growth speed and regeneration, and has excellent production performance under the condition of sufficient water and fertilizer.

[0036] (2) Carry out reciprocal crossing of the two types of parental materials in step (1) according to conventional methods, and screen and reserve seeds according to conventional methods in combination with yield, disease resistance, cold resistance and active ingredient content from the hybrid lines produced.

[0037] (3) Soak the seeds obtained in step (2) with warm water at 40°C for 24 hours, then dry or air-dry naturally to obtain dried see...

technical field

[0001] The invention relates to the field of Chinese herbal medicine biotechnology breeding, in particular to Isatis indica 12 C 6+ Ion beam radiation breeding method. Background technique

[0002] As a new radiation mutagenic source, heavy ion beam has the characteristics of high energy transmission line density. In plant germplasm mutation breeding operations, its mutagenic efficiency per unit dose is 10 times higher than that of X-rays, γ-rays, electron beams, etc. , resulting in a significant increase in the proportion of double-strand breaks in DNA molecules in the nucleus, showing a more serious degree of damage, a high mutation rate, and the damage is not easy to repair, and the mutant is stable. These characteristics have great advantages in mutation breeding, which can achieve the purpose of mutagenesis with a small dosage, and have high mutation rate, good stability of mutants and short breeding cycle. The heavy ion parameters are diverse, which...

Examples

Embodiment 1

[0018] Example 1 Woad 12 C 6+ Ion beam radiation breeding method, comprising the following steps:

[0019] (1) Two types of parental materials were selected according to the breeding objectives: one is local germplasm with strong stress resistance selected from local varieties, and used as the source of drought-tolerant genes for hybrid offspring in breeding; The selected breeding line has excellent growth speed and regeneration, and has excellent production performance under the condition of sufficient water and fertilizer.

[0020] (2) Carry out reciprocal crossing of the two types of parental materials in step (1) according to conventional methods, and screen and reserve seeds according to conventional methods in combination with yield, disease resistance, cold resistance and active ingredient content from the hybrid lines produced.

[0021] (3) Soak the seeds obtained in step (2) with warm water at 30°C for 36 hours, then dry or air-dry naturally to obtain dried se...

Embodiment 2

[0026] Example 2 Woad 12 C 6+ Ion beam radiation breeding method, comprising the following steps:

[0027] (1) Two types of parental materials were selected according to the breeding objectives: one is local germplasm with strong stress resistance selected from local varieties, and used as the source of drought-tolerant genes for hybrid offspring in breeding; The selected breeding line has excellent growth speed and regeneration, and has excellent production performance under the condition of sufficient water and fertilizer.

[0028] (2) Carry out reciprocal crossing of the two types of parental materials in step (1) according to conventional methods, and screen and reserve seeds according to conventional methods in combination with yield, disease resistance, cold resistance and active ingredient content from the hybrid lines produced.

[0029] (3) Soak the seeds obtained in step (2) with warm water at 50° C. for 12 hours, then dry or air-dry naturally to obtain dried ...

Embodiment 3

[0034] Example 3 Woad 12 C 6+ Ion beam radiation breeding method, comprising the following steps:

[0035](1) Two types of parental materials were selected according to the breeding objectives: one is local germplasm with strong stress resistance selected from local varieties, and used as the source of drought-tolerant genes for hybrid offspring in breeding; The selected breeding line has excellent growth speed and regeneration, and has excellent production performance under the condition of sufficient water and fertilizer.

[0036] (2) Carry out reciprocal crossing of the two types of parental materials in step (1) according to conventional methods, and screen and reserve seeds according to conventional methods in combination with yield, disease resistance, cold resistance and active ingredient content from the hybrid lines produced.

[0037] (3) Soak the seeds obtained in step (2) with warm water at 40°C for 24 hours, then dry or air-dry naturally to obtain dried see...