Ion beam injection mutation breeding method for cordyceps militaris strains and cordyceps militaris strains bred therethrough

An ion beam injection, Cordyceps militaris technology, applied in the directions of botanical equipment and methods, fungal products, plant genetic improvement, etc., can solve the problem that microbial strains cannot be copied and applied, and achieve the effect of excellent production performance

Active Publication Date: 2015-09-09
BEIJING FORESTRY UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although ion beam implantation mutagenesis is a known technology in the art, it has been reported in the literature that ion beam implantation mutagenesis is used to breed fungal strains, but different types of microorganisms have different tolerances to ion beam implantation mutagenesis energy and doses. , even among different strains of the same strain, there are still large differences, so there is an optimal mutagenic dose range for each strain, under which the mutation rate of the strain is the highest, and more The acquisition of this optimal condition is of great significance to the mutagenesis selection of strains, and because the individual differences of microbial strains cannot be copied and applied, it must be optimized through a large number of experiments to obtain

Method used

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  • Ion beam injection mutation breeding method for cordyceps militaris strains and cordyceps militaris strains bred therethrough
  • Ion beam injection mutation breeding method for cordyceps militaris strains and cordyceps militaris strains bred therethrough
  • Ion beam injection mutation breeding method for cordyceps militaris strains and cordyceps militaris strains bred therethrough

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Embodiment 1

[0040] This embodiment is used to illustrate the screening process of the Cordyceps militaris strain provided by the present invention.

[0041] 1. Strains:

[0042] Starting strain: Cordyceps militaris (Cordyceps militaris) No.: CM-3, preserved in the Microbiological Laboratory of Beijing Radiation Center.

[0043] 2. Medium and reagents:

[0044] PDA solid medium: glucose 20g, potassium dihydrogen phosphate 1g, potato juice 1000mL, pH natural, agar 20g, sterilized at 121°C for 30min.

[0045] Liquid medium: glucose 30g, tryptone 4g, yeast powder 2g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, pH natural, heated to dissolve, distilled water to 1000mL, sterilized at 121°C for 30min.

[0046] Reagents: Glucose, potassium dihydrogen phosphate, and magnesium sulfate are all analytical reagents, purchased from Sinopharm Beijing Chemical Reagent Company; cordycepin is HPLC pure grade, purchased from Sigma Company; tryptone, yeast powder, and agar The grade of biol...

Embodiment 2

[0087] 1. Solid culture of Cordyceps militaris strain

[0088] The solid culture of the Cordyceps militaris bacterial strain adopts liquid seeds, and the preparation method of the seed liquid is as follows: use a solid plate strain, punch into inoculation pieces of the same size with a puncher, inoculate 5 pieces in each bottle, put 100mL seed liquid culture medium in a 250mL triangular bottle, and formula As follows, culture in a shaker at a constant temperature of 25°C with a rotation speed of 150r / min and a culture time of 5 days.

[0089] The formula of Cordyceps militaris strain seed liquid culture medium is: glucose 30g, tryptone 4g, yeast powder 2g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, pH is natural, heated to dissolve, distilled water to 1000mL, 121 ℃, sterilized for 30min .

[0090] The formula of Cordyceps militaris solid medium is: 60g of oats (rice, barley, wheat, millet, sorghum, etc. can also be used), add 80mL of nutrient solution, sterili...

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Abstract

The invention relates to a cordyceps militaris strain preserved in CGMCC on Oct. 22th, 2014, with the preservation number thereof to be CGMCC No. 9818. The invention also provides the application of the above strain in the liquid fermentation and production of cordycepin and cordyceps militaris mycelium and the solid culture and production of cordycepin, and the ion beam injection mutation breeding method for cordyceps militaris strains. The cordyceps militaris strain is excellent in cordycepin production performance, and the cordycepin yield of the above strain is higher than that of an original strain by 8.85 times.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to a method for ion beam implantation mutagenesis and breeding of Cordyceps militaris strains and the bred strains. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, is one of the traditional important edible and medicinal fungi in my country, and it is also a new resource food approved by the Ministry of Health. It is now called a new food raw material and is allowed to be eaten directly as food. Cordycepin is the most important biological active ingredient of Cordyceps militaris, which has the functions of immune regulation, antitumor, antibacterial, antiviral, and treatment of leukemia. Cordycepin is expensive, and chemical synthesis can be realized in the laboratory at present, but due to limited conditions, it cannot be produced. Therefore, the cordycepin sold in the market is mainly extracted and isolated from the culture of Cordycep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H15/00A01G1/04
Inventor 刘桂君尚宏忠周思静顾海科王平杨素玲孟佑婷郑洁张丰收
Owner BEIJING FORESTRY UNIVERSITY
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