Method for culturing cordyceps militaris mycelium and plasmin

Abstract

The invention discloses a method for culturing cordyceps militaris mycelium and plasmin. The used cordyceps militaris bacterial strain has a preservation number of CGMCC No.2577 in the General Microbiological Center of China Committee of Culture Collection for Microorganisms on 4th, July, 2008. The taxology name is cordyceps militaris. The culture method comprises bacterial classification activation, seed nutrient solution preparation and solid fermentation, a solid fermentation matrix contains cordyceps militaris and plasmi, its plasmin vitality can reach 927.77U / g. The method uses rice and wheat bran as culture matrix for culturing cordyceps militaris and producing cordyceps militaris mycelium and plasmin, bacterial classification and production raw material are safe and reliable, the raw material has the advantages of low cost and easy acquisition, compared with a traditional cultivation technology, the production period is shortened by 5 / 6, operation is simple, and realization is easy. The method provides a novel thinking and research base for research and development of health food for preventing and treating pulmonary thromboembolism, and provides a novel approach for producing cordyceps militaris mycelium.

Description

technical field

[0001] The invention relates to a method for jointly cultivating a kind of Cordyceps militaris mycelium and plasmin, belonging to the field of food science and technology. Background technique

[0002] Biothrombolysis is currently the main method for the treatment and prevention of thrombotic diseases. It is an urgent need for modern medicine to develop new thrombolytic drugs that are efficient, fast, prevent re-embolization, and reduce adverse reactions such as bleeding. Cordyceps militaris is a precious traditional Chinese medicine and high-grade tonic, which can improve immune function, protect the liver and clear the lungs, relieve cough and reduce phlegm, resist fatigue, resist hypoxia, and resist viruses. For more than ten years, the applicant has been exploring the application potential of Cordyceps militaris in the preparation of modern biothrombolytic agents, and successfully screened a Cordyceps militaris strain C.LSG with excellent thrombolytic pro...

Examples

Embodiment 1

[0022] 1. Strain: Cordyceps militaris strain C.LSG-1, the preservation number is CGMCC N O .2577。

[0023] 2. Medium and culture conditions:

[0024] (1) Incline medium: 20% potato juice, 2.5% agar, 1% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, natural pH.

[0025] Culture conditions: Culture at 23°C for 15-20 days.

[0026] (2) Plate medium: 20% potato juice, 2.5% agar, 1% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, natural pH.

[0027] Culture conditions: Culture at 23°C for 15-20 days.

[0028] (3) Liquid seed medium: 20% potato juice, 1% glucose, 0.5% peptone, natural pH.

[0029] Culture conditions: 23°C, 150r / min for 3 days.

[0030] (4) Solid fermentation medium: composed of rice and bran, the ratio is 3:5, the amount of water added to the medium is 70-80% (v / w), and the initial pH of the medium is natural; the amount of liquid seeds inoculated with Cordyceps mil...

Embodiment 2

[0033] 1, bacterial classification: with embodiment 1.

[0034] , medium and culture conditions:

[0035] (1) Incline cultivation is the same as in Example 1.

[0036] (2) Plate culture is the same as in Example 1.

[0037] (3) Liquid seed medium: 20% potato juice, 1-2% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, natural pH.

[0038] Culture conditions: 23°C, 150r / min for 3 days.

[0039] (4) Solid fermentation medium: composed of rice and bran, the ratio is 3:5, the amount of water added to the medium is 70-80% (v / w), and the initial pH of the medium is natural; the amount of liquid seeds inoculated with Cordyceps militaris is the dry medium 10% by weight (v / w).

[0040] Culture conditions: 23°C constant temperature and humidity culture time 4d.

Embodiment 3

[0042] 1, bacterial classification: with embodiment 1.

[0043] , medium and culture conditions:

[0044] (1) Incline cultivation is the same as in Example 1.

[0045] (2) Plate culture is the same as in Example 1.

[0046] (3) Liquid seed medium: 20% potato juice, 2% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, natural pH, inoculation amount is a piece of bacterial lawn disk with a diameter of 10mm.

[0047] Culture conditions: 23°C, 180r / min for 4 days.

[0048] (4) Solid fermentation medium: composed of rice and bran, the ratio is 3:5, the amount of water added to the medium is 70-80% (v / w), and the initial pH of the medium is natural; the amount of liquid seeds inoculated with Cordyceps militaris is the dry medium 10% by weight (v / w).

[0049] Culture conditions: 23°C constant temperature and humidity culture time 4d.

technical field

[0001] The invention relates to a method for jointly cultivating a kind of Cordyceps militaris mycelium and plasmin, belonging to the field of food science and technology. Background technique

[0002] Biothrombolysis is currently the main method for the treatment and prevention of thrombotic diseases. It is an urgent need for modern medicine to develop new thrombolytic drugs that are efficient, fast, prevent re-embolization, and reduce adverse reactions such as bleeding. Cordyceps militaris is a precious traditional Chinese medicine and high-grade tonic, which can improve immune function, protect the liver and clear the lungs, relieve cough and reduce phlegm, resist fatigue, resist hypoxia, and resist viruses. For more than ten years, the applicant has been exploring the application potential of Cordyceps militaris in the preparation of modern biothrombolytic agents, and successfully screened a Cordyceps militaris strain C.LSG with excellent thrombolytic pro...

Examples

Embodiment 1

[0022] 1. Strain: Cordyceps militaris strain C.LSG-1, the preservation number is CGMCC N O .2577。

[0023] 2. Medium and culture conditions:

[0024] (1) Incline medium: 20% potato juice, 2.5% agar, 1% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, natural pH.

[0025] Culture conditions: Culture at 23°C for 15-20 days.

[0026] (2) Plate medium: 20% potato juice, 2.5% agar, 1% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, natural pH.

[0027] Culture conditions: Culture at 23°C for 15-20 days.

[0028] (3) Liquid seed medium: 20% potato juice, 1% glucose, 0.5% peptone, natural pH.

[0029] Culture conditions: 23°C, 150r / min for 3 days.

[0030] (4) Solid fermentation medium: composed of rice and bran, the ratio is 3:5, the amount of water added to the medium is 70-80% (v / w), and the initial pH of the medium is natural; the amount of liquid seeds inoculated with Cordyceps mil...

Embodiment 2

[0033] 1, bacterial classification: with embodiment 1.

[0034] , medium and culture conditions:

[0035] (1) Incline cultivation is the same as in Example 1.

[0036] (2) Plate culture is the same as in Example 1.

[0037] (3) Liquid seed medium: 20% potato juice, 1-2% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, natural pH.

[0038] Culture conditions: 23°C, 150r / min for 3 days.

[0039] (4) Solid fermentation medium: composed of rice and bran, the ratio is 3:5, the amount of water added to the medium is 70-80% (v / w), and the initial pH of the medium is natural; the amount of liquid seeds inoculated with Cordyceps militaris is the dry medium 10% by weight (v / w).

[0040] Culture conditions: 23°C constant temperature and humidity culture time 4d.

Embodiment 3

[0042] 1, bacterial classification: with embodiment 1.

[0043] , medium and culture conditions:

[0044] (1) Incline cultivation is the same as in Example 1.

[0045] (2) Plate culture is the same as in Example 1.

[0046] (3) Liquid seed medium: 20% potato juice, 2% glucose, 0.5% peptone, 0.1% potassium dihydrogen phosphate, 0.05% magnesium sulfate, natural pH, inoculation amount is a piece of bacterial lawn disk with a diameter of 10mm.

[0047] Culture conditions: 23°C, 180r / min for 4 days.

[0048] (4) Solid fermentation medium: composed of rice and bran, the ratio is 3:5, the amount of water added to the medium is 70-80% (v / w), and the initial pH of the medium is natural; the amount of liquid seeds inoculated with Cordyceps militaris is the dry medium 10% by weight (v / w).

[0049] Culture conditions: 23°C constant temperature and humidity culture time 4d.

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