67 results about "Rhodotorula" patented technology

The invention discloses a rhodotorula mucilaginosa strain as well as a fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms. The rhodotorula mucilaginosa BTN-HB-L28 strain is separated and purified from meconopsis in Lijiang of Yunnan, can be used in the field of cosmetics, has the effects of resisting skin oxidation, resisting aging, repairing skin and relieving skin, can be used as an active matter in skin care products, and has practical application value. The fermentation product filtrate of the strain has excellent HAS inhibition ability, and can significantly inhibit the expression of matrix metalloproteinase 3; the fibroblast proliferation can be obviously promoted under various concentrations; the proportion of SA-beta-Gal positive cells can be remarkably reduced under various concentrations, and the anti-aging effect is achieved; the antioxidant composition has remarkable DPPH and ABTS free radical scavenging capacity and has a good antioxidant effect; the secretion amount of cell inflammatory factors NO, IL-6 and TNF-alpha can be remarkably inhibited, and the good relieving effect is achieved.

The invention provides a phosphorus release improving type compound fertilizer based on a fungal extracellular polymer, a preparation method and application, and belongs to the technical field of microorganisms, the phosphorus release improving type compound fertilizer is prepared by compounding the extracellular polymer generated by specific rhodotorula mucilaginosa WTSX01 and inorganic phosphate according to the mass ratio of 1: 9; the preparation method comprises the following steps: extracting and purifying high-activity EPS from a strain fermentation broth by a low-temperature dialysis method, separating lipid from EPS as a natural adhesive by a cosolvent extraction method, and finally mixing EPS, lipid and inorganic phosphate. The invention also discloses a synergistic application of the fertilizer and a phosphorus-solubilizing fungus aspergillus niger (CCTCC No. M2023240), so that the release efficiency of phosphorus in soil is improved; the fertilizer provided by the invention provides exclusive nutrition for phosphate-solubilizing microorganisms, effectively overcomes indigenous microorganism competition, remarkably promotes microorganism growth and phosphorus release, and realizes reduction and synergism of phosphate fertilizer and yield increase of crops.

The invention relates to a rhodotorula mucilaginosa strain and a yellow wine co-fermentation process thereof, and belongs to the technical field of fermentation engineering and strains. The invention provides the rhodotorula mucilaginosa with the fermentation characteristics of low temperature resistance, acid resistance, high yield of beta-glucosidase and aroma production, sequential inoculation is adopted, the rhodotorula mucilaginosa H04 is firstly inoculated for fermentation for two days, then the saccharomyces cerevisiae is inoculated, and the yellow wine co-fermentation is carried out by an equal-proportion 20 DEG C low-temperature fermentation process. The yellow rice wine meeting national standard requirements of yellow rice wine can be obtained after 20 days of fermentation, and the volatile flavor type, content, taste and aroma sensory quality of the yellow rice wine after fermentation are improved.

The invention belongs to the technical field of microbial fermentation, and particularly relates to rhodotorula mucilaginosa JQRM001 and application thereof. The invention provides a rhodotorula mucilaginosa JQRM001, the Latin name of the rhodotorula mucilaginosa JQRM001 is Rhodotorula mucilaginosa, the rhodotorula mucilaginosa JQRM001 is preserved in China General Microbiological Culture Collection Center, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 15, 2025, and the preservation number is CGMCC No.35240. The invention further provides a preparation method of the rhodotorula mucilaginosa. The rhodotorula mucilaginosa JQRM001 not only has excellent performance of high temperature resistance and acid resistance, but also can be used for specifically synthesizing alkylpyrazine flavor substances and remarkably improving the antioxidant activity of plant polysaccharides.

The invention belongs to the technical field of sewage treatment, and particularly relates to an urban domestic sewage treatment method. Chitosan, modified activated carbon and a complex microbial inoculant formed by compounding any two strains of spirulina, rhodotorula mucilaginosa and micrococcus luteus are specifically combined, the chitosan in the composition serves as a carrier of the complex microbial inoculant, nutrients can be provided for survival of the complex microbial inoculant, and the survival time of the microbial inoculant is prolonged; the modified activated carbon has high adsorption capacity on the complex microbial inoculant, so that slow release of the complex microbial inoculant in the later period is facilitated; therefore, the composition provided by the invention can be used for purifying the urban domestic sewage, the purpose of purifying the urban domestic sewage is achieved, ecologicalization and recycling of domestic sewage treatment are realized, and a new thought is provided for urban sewage treatment.

The invention discloses Rhodosporidium toruloides for aquatic feed and application of the Rhodosporidium toruloides to aquaculture, relates to the technical field of microorganisms, and particularly relates to Rhodosporidium toruloides Ho7-3 which can be applied to aquaculture, has the effects of improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system, and can be used for improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system. The growth performance of cultured fishes can be obviously improved.

The invention relates to the technical field of microorganisms, in particular to a method for producing astaxanthin emulsion from phaffia rhodozyma, which specifically comprises the following steps of: performing superfine grinding on dried phaffia rhodozyma, extracting astaxanthin by using a solvent, dissolving the astaxanthin in soybean oil, taking whey protein as a water phase, preparing O / W emulsion, adding tannic acid to protect the astaxanthin from being influenced by enzymatic crosslinking, and preparing the astaxanthin emulsion by using the tannic acid. And a'multilayer 'interfacial film is formed through electrostatic assembly and enzymatic crosslinking. The chitosan-sodium alginate'multi-layer 'interfacial film improves the mechanical strength of the interfacial film, and limits the oxidizing agent from permeating into the internal oil phase to be in contact with the astaxanthin. Flocculation of the astaxanthin emulsion is inhibited through the charge neutralization effect of sodium alginate grafted polyethylene glycol sheltered salt ions, enzymatic crosslinking is conducted on chitosan grafted peptide fragments, and targeted release of the astaxanthin in the intestinal tract is achieved. The use amount of tyrosinase and the enzymatic reaction time are controlled, the residue of tyrosinase with oxidizing property is prevented, and tannic acid prevents the oxidizing substance generated by the reaction of tyrosinase from oxidizing astaxanthin.

This invention relates to a high-yield coenzyme Q method. 10 The establishment and application of *Rhodotorula globulinii* strains. Compared to rational metabolic engineering, the coenzyme Q of this invention... 10 High-yielding Rhodopseudomonas aeruginosa bacteria were used as a template by the global regulatory factor prrA gene. Through directed evolution, different mutant strains were constructed, and combined with high-throughput screening methods, coenzyme Q was achieved. 10 Increased yield. This invention starts from global regulatory factors and leverages complex intracellular regulatory mechanisms to achieve fine-grained global regulation under minute genomic perturbations, providing a new approach for the engineering modification of strains.

The application discloses a kind of biological inoculums for improving fermentation environment of waste pool of waste incineration plant, solve high kitchen garbage heavy metal pollution problem.It uses "functional strain + kitchen waste oil modified biochar carrier" collaborative design, functional strain includes Yarrowia lipolytica, Aspergillus oryzae, salt-tolerant lactic acid bacteria and Rhodotorula, Rhodotorula can efficiently passivate Pb 2+ , Cd 2+ , strain synergistic degradation of oil and starch.Application of percolate soluble heavy metal content reaches standard, reduces heavy metal treatment reagent dosage 25%-30%, reduces fly ash "hazardous waste" disposal ratio, realizes fermentation improvement and heavy metal passivation integration, with environmental protection and economic benefits.

The application discloses a gastrointestinal delivery carrier of high-activity compound of Rhodotorula and application thereof in colon fermentation, and belongs to the field of food science, pharmaceutical preparation, nutrition and microbiology research. The application explores the storage stability and digestive tract metabolism of the high-activity compound of Rhodotorula in different carrier forms, namely corn oil loaded with the high-activity compound of Rhodotorula (F-oil), spray-dried powder of corn oil nanoemulsion of the high-activity compound of Rhodotorula (F-EP) and alginate gel of corn oil nanoemulsion of the high-activity compound of Rhodotorula (F-EPA), wherein the storage stability of F-EPA is the best. The three carriers in the application do not release the high-activity compound of Rhodotorula in the oral cavity and stomach stages in vitro, but in the small intestine stage, the digestion rate and bioavailability of F-EP are the highest, and the release of F-EPA is the least. F-EPA can effectively promote the regulation of intestinal microbial community structure and abundance value by Rhodotorula rubin.

This invention discloses a glucose phosphate mutase gene Rk PGM Its nucleotide sequence is shown in SEQ ID NO:1, and the amino acid sequence encoded by this gene is shown in SEQ ID NO:2; this gene was isolated from *Rhodotorula rubrum* (…). Rhodosporidium kratochvilovae YM25235 was used to ligate the gene to a vector and transfected into Rhodotorula rubrum cells. Experimental results showed that overexpression of Rk... PGM The gene will increase the level of extracellular polysaccharide synthesis in this strain; this invention modifies microorganisms through genetic engineering to improve their ability to secrete extracellular polysaccharides, providing a theoretical basis for increasing the yield of extracellular polysaccharides in Rhodotorula rubrum.

This invention relates to the field of genetic engineering technology and provides a method for increasing erythromycin yield by modifying the SACE_5680 gene of *Rhodotorula sacchariformis*. The method involves deleting the GntR family SACE_5680 gene in *Rhodotorula sacchariformis* through genetic engineering, thereby obtaining a high-yielding engineered strain of *Rhodotorula sacchariformis* for erythromycin production. Erythromycin is then produced by fermentation using this high-yielding engineered strain. The nucleotide sequence of the SACE_5680 gene is shown in SEQ ID NO. 1. This invention also provides an application of the above method. In this study, the negative regulator of erythromycin biosynthesis, SACE_5680, was screened. By deleting a copy of the SACE_5680 gene on the chromosome of *Rhodotorula sacchariformis* through genetic engineering, a high-yielding strain of erythromycin can be obtained, providing technical support for increasing erythromycin fermentation yield in industrial production.

The invention discloses rhodotorula glutinis LUCNOVA 002 as well as a zymophyte agent and application thereof. The preservation number of the rhodotorula glutinis LUCNOVA 002 is CCTCC (China Center for Type Culture Collection) NO: M2022812. The fermentation bacterial agent is prepared from a combination of rhodotorula glutinis LUCNOVA 002 and saccharomyces cerevisiae NJ-LK12. According to the invention, the rhodotorula glutinis LUCNOVA 002 is obtained through screening, a liquid-solid combined deep fermentation technology is fully utilized, a fermentation inoculant composed of composite seed liquid of rhodotorula glutinis and saccharomyces cerevisiae is used for fermentation of the flaxseed-soybean meal compound, the production raw materials are sufficient, the production process is simple, the production cost is low, and the flaxseed-soybean meal compound is convenient to store and use.

According to the marine rhodotorula mucilaginosa and the method for mixed inoculation of the marine rhodotorula mucilaginosa and the lactobacillus rhamnosus, the selected marine rhodotorula mucilaginosa can efficiently degrade NDMA in air-dried sausages and smelly mandarin fish, various nitrosamines in fermented meat products can be jointly degraded through the synergistic effect of the marine rhodotorula mucilaginosa and the NDMA in the smelly mandarin fish, in addition, the mixed bacteria synergistically show an excellent degradation effect on biogenic amines, and the marine rhodotorula and the degradation efficiency is far higher than that of single-bacterium fermentation. In the invention, the combination of adaptive directed evolution and mutation breeding is used for improving the nitrosamine degradation capability of the strain for the first time, the marine rhodotorula mucilaginosa SY-AN-9 obtained by the technology is applied to degradation of nitrosamine in fermented meat products for the first time, and a method for synergistically degrading biogenic amine and nitrosamine by mixed bacteria is developed. The safety of the fermented meat product is improved, the application range of the marine rhodotorula mucilaginosa and the rhamnosus lactobacillus is widened, and the application of the marine rhodotorula mucilaginosa and the rhamnosus lactobacillus in the food industry is promoted.

The invention relates to Rhodotorula paludi and an application of the Rhodotorula paludi in aroma enhancement and quality improvement of a tobacco stem concentrated solution, the Rhodotorula paludi is named as a Rhodotorula paludi H72 strain, the preservation number is CGMCC No.34875, and the preservation date is June 12, 2025. The Rhodotorula paludi is named as Rhodotorula paludi H72 strain, the preservation number is CGMCC No.34875, and the preservation number is CGMCC No.34875. According to the invention, a new Rhodotorula paludigena strain is separated from cigar tobacco leaves and is preserved, the new Rhodotorula paludigena strain is named as Rhodotorula paludigena strain H72, and the strain can significantly improve the relative content of aroma substances in the tobacco stem concentrated solution and increase new aroma substances, significantly improve the sensory quality of the tobacco stem concentrated solution, and improve the quality of the tobacco stem concentrated solution. The strain or the microbial inoculum can be used for preparing tobacco products after fermenting a tobacco stem concentrated solution or a diluent thereof, so that the aroma and the quality of the tobacco products are improved.

Capsule structure (C) with a red yeast compound, comprising: - a capsule body (10) comprising a first capsule shell (11) and a second capsule shell (12), wherein the first capsule shell (11) and the second capsule shell (12) are placed on top of each other in such a way that they together enclose a closed receiving space (13); and - several functional particles (20) arranged in the receiving space (13) of the capsule body (10), wherein the composition of the functional particles (20) includes red yeast (21), nattokinase (22) and coenzyme Q10 (23).

The invention belongs to the technical field of tobacco strains, and particularly relates to a rhodotorula mucilaginosa H04 strain and application thereof in preparation of tobacco flavor. The rhodotorula mucilaginosa H04 provided by the invention has excellent aroma producing performance and can produce tobacco characteristic aroma substances such as alcohols, aldehydes, esters and pyrazines at high yield. The rhodotorula mucilaginosa H04 has good tolerance to nicotine, can still keep high growth activity under the condition that the nicotine concentration is as high as 8 mg / mL, and is suitable for being used for preparing tobacco flavor; the tobacco flavor prepared by the invention contains a plurality of natural aroma components, has good coordination with the original aroma of tobacco, and can significantly improve the aroma richness and comfort of tobacco products; the highest beta-glucosidase activity of the rhodotorula mucilaginosa H04 reaches 464.0 + / -24.4 U / mL, and the laccase activity reaches 60.4 + / -3.2 U / mL, so that the release of bound aroma precursor substances in tobacco is facilitated, and the aroma expression is enhanced.

The application discloses a furaldehyde-tolerant Rhodotorula mucilaginosa strain and application thereof, the strain is preserved in the China General Microbiological Culture Collection Center, the preservation date is July 1, 2024, the preservation number is CGMCC NO.31122, and the Rhodotorula mucilaginosa strain is named Rhodotorula mucilaginosa F3.The furaldehyde concentration that can be tolerated by the Rhodotorula mucilaginosa F3 strain is 40mM-45mM, so that the production cost of carotenoids can be reduced, the utilization efficiency of lignocellulose hydrolysate is improved, waste utilization is realized, and good commercial and scientific research values are obtained.

The invention discloses rhodotorula mucilaginosa TGy002 and application thereof in enrichment and selenium conversion at low temperature, and belongs to the technical field of microorganisms, the collection number of the rhodotorula mucilaginosa TGy002 is CCTCC NO: M 20251010, the collection site is China Center for Type Culture Collection, and the collection date is May 9, 2025. The rhodotorula mucilaginosa strain TGy002 developed by the invention can maintain the activity of thalli and the stability of metabolites under a low-temperature condition, improves the product quality and stability, not only can solve the problem of non-uniform distribution of selenium resources, but also can provide a safe and efficient organic selenium source for cold regions, and has a wide application prospect.

This invention relates to an antibiotic-label-free, induced, salt-tolerant Rhodotorula globulinii engineered strain and its applications, belonging to the field of microbial technology. Wild Rhodotorula globulinii is a dominant species for wastewater biological treatment, but natural strains grow extremely slowly at salt concentrations exceeding 3%, significantly inhibiting their ability to degrade pollutants. The induced, salt-tolerant Rhodotorula globulinii engineered strain constructed in this invention, after lactose induction, significantly improves the treatment capacity of ammonia nitrogen, phosphate, and acetate in 3%–5% high-salt wastewater compared to wild strains and constitutive overexpression engineered strains. This provides technical support for the application of Rhodotorula globulinii in coastal industrial wastewater purification, saline-alkali land ecological restoration, and seawater recycling, significantly enhancing the competitiveness of Rhodotorula globulinii in low-cost seawater cultivation, high-salt wastewater treatment, and biomanufacturing in special environments.

The application discloses a biomass waste residue recycling method. The biomass waste residue recycling method comprises the following steps: mixing filamentous fungi and yeast with biomass waste residue, carrying out fermentation culture, carrying out solid-liquid separation, and extracting biomass oil from the separated solid. The filamentous fungi comprise one or a combination of two selected from Trichoderma reesei and Aspergillus niger; the yeast comprises one or a combination of two selected from Lipomyces starkeyi and Rhodotorula. The biomass waste residue recycling method can treat fruit residue, coffee residue or medicine residue to produce biomass oil.

The invention discloses a biomass waste residue recycling method. The biomass waste residue recycling method comprises the following steps: mixing filamentous fungi and saccharomycetes with biomass waste residues, carrying out fermentation culture, carrying out solid-liquid separation, and extracting biomass oil from separated solids, wherein the filamentous fungi comprise one or a combination of two of trichoderma reesei and aspergillus niger; the saccharomycetes comprise one or a combination of more selected from lipomyces stutzeri and rhodotorula. The biomass waste residue recycling method provided by the invention can be used for treating fruit residues, coffee residues or medicine residues to produce biomass oil.

The invention belongs to the technical field of microorganisms, and particularly relates to a new strain of microbacterium and application thereof. The bacterial strain is separated from rapana venosa, is a new species of Microbacterium sp. M-28, is registered and preserved in the China Center for Type Culture Collection (CCTCC), and has a preservation number of CCTCC NO: M 20241861. The invention also discloses a method for preparing the bacterial strain. The microbacterium sp. M-28 strain disclosed by the invention has obvious bacteriostatic activity on bacillus subtilis, shewanella, staphylococcus aureus and proteusbacillus vulgaris, has obvious acetylcholin esterase inhibitory activity, has obvious antioxidant activity, has alpha-glucosidase inhibitory activity, can generate casein enzyme and amylase, and can be used for preparing a food additive. The strain can be applied to preparation of a bacteriostatic agent, an acetylcholin esterase inhibitor, an antioxidant, an alpha-glucosidase inhibitor, casein and amylase, can also replace the bacteriostatic agent, the antioxidant, the casein and the amylase in a viable form to be applied to industrial production, and has a very good development and application prospect.

The invention discloses a microecological preparation for trachinotus ovatus as well as a preparation method and application of the microecological preparation. The preparation consists of 80-95 parts of a fermented soybean meal carrier, 1-5 parts of clostridium butyricum zymophyte powder, 1-5 parts of rhodotorula zymophyte powder and 1-5 parts of bacillus coagulans zymophyte powder. The preparation method comprises the following steps: respectively preparing the components and uniformly mixing. Aiming at the physiological characteristics and breeding pain points of the golden pomfret, the deep fermented soybean meal is innovatively used as a functional carrier, and special probiotics with butyric acid production, immunity enhancement and stress tolerance characteristics are compounded to form a synergy synbiotic product. When the preparation is added into the trachinotus ovatus feed, the growth of trachinotus ovatus can be remarkably promoted, the feed utilization rate is increased, the immunity of the organism is enhanced, the intestinal health is optimized, the culture water quality is improved, and the preparation has remarkable economic and ecological benefits.

This invention discloses a thermophilic recombinant *Yersinia lipolytica* strain producing carotenoids, its construction method, and its applications. The recombinant strain exogenously expresses thermophilic precursor fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from *Rhodotorula rubrum*, achieving efficient synthesis at 30°C. Secondly, the metabolic pathway of the recombinant strain is optimized based on a metabolic flux push-pull strategy, overexpressing isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), phytene dehydrogenase (CarB), and bifunctional phytene synthase (CarRP) to increase precursor throughput. Finally, multiple copies of the genes for astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from *Rhodotorula rubrum* are performed to further enhance the carotenoid production capacity of *Yersinia lipolytica*. The thermophilic *Yersinia lipolytica* construction method of this invention is simple, can achieve more efficient carotenoid synthesis at higher temperatures, significantly reduces fermentation costs, and has potential for industrial production.

The application discloses a microbial compound microbial agent and a preparation method and application of Chinese herbal medicine compound microbial agent in offshore fish culture, and belongs to the technical field of microbial engineering. The microbial compound microbial agent comprises bacillus megaterium and rhodotorula mucilaginosa. In addition, the application further provides a preparation method of the Chinese herbal medicine compound microbial agent, which comprises the following steps: mixing the microbial compound microbial agent, Chinese herbal medicine and base fluid and then fermenting. In addition, the application further provides a Chinese herbal medicine compound microbial agent prepared by the preparation method. In addition, the application further provides a feed, and the mass addition amount of the microbial compound microbial agent prepared by the preparation method in the feed is 5%-10%. In addition, the application further provides application of the Chinese herbal medicine compound microbial agent prepared by the preparation method in offshore fish culture. The microbial compound microbial agent can be used for Chinese herbal medicine fermentation, and after being fed to fish, the fish obesity problem caused by artificial sweeteners can be effectively improved.

The invention discloses a streptomyces noursei YC3078 strain and application thereof in production of gougerotin, and belongs to the technical field of biology. The preservation number of the streptomyces noursei YC3078 provided by the invention is GDMCC No: 67402, the strain is constructed by overexpressing a CMP gene in streptomyces albidus, and the nucleotide sequence of the CMP gene is as shown in SEQ ID NO: 1. Experiments show that the strain not only significantly improves the content of the gougerotin in a fermentation product, but also improves the utilization efficiency of ammonia nitrogen and glucose in fermentation liquor, and the inhibition ability of the gougerotin produced by fermentation of the strain to rhodotorula is significantly superior to that of a wild strain. According to the invention, efficient strain resources are provided for large-scale production of the gougerotin, and scientific basis and technical support are provided for research and development of agricultural antibiotics and prevention and treatment of plant diseases.

The invention discloses a transcription auxiliary repressor protein gene RkTup1. The nucleotide sequence of the transcription auxiliary repressor protein gene RkTup1 is as shown in SEQ ID NO: 1; a knockout gene RkTup1 plasmid is constructed according to the gene and is transferred into rhodosporidium kratochvilovae YM25235, an experimental result shows that compared with wild rhodosporidium kratochvilovae YM25235, the grease synthesis amount of an obtained RkTup1 gene knockout strain is remarkably increased, and the result shows that a gene knockout engineering bacterium constructed based on the gene provided by the invention can be used for grease synthesis; the method has important theoretical significance and potential economic value for research on regulation and control of grease synthesis.

The invention discloses rhodotorula mucilaginosa and application of the rhodotorula mucilaginosa in kiwi fruit wine, and belongs to the technical field of microorganisms. The rhodotorula mucilaginosa M07 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center on October 24, 2024, and the preservation number is CGMCC No.32331. The rhodotorula mucilaginosa M07 disclosed by the invention has the advantages that the rhodotorula mucilaginosa In a strain tolerance determination result, the strain M07 can tolerate the pH value of 3.0, the sugar concentration of 450 g / L and the alcohol concentration of 4%. The concentration of ethyl acetate, ethyl hexanoate, ethyl caprylate, phenethyl alcohol and ethyl caprate in kiwi fruit wine brewed by the rhodotorula mucilaginosa strain is improved compared with that of Angel fruit wine yeast, and the relative content of ethyl acetate can reach 20.033%; in the kiwi fruit wine fermented by the composite yeast, the two yeasts can interact with each other to adjust the content of each substance in the wine; the rhodotorula mucilaginosa fermented kiwi fruit wine has a certain positive effect on the flavor of the kiwi fruit wine.

The invention belongs to the field of biological information detection, and provides a multiplex PCR (polymerase chain reaction) detection primer group for simultaneously detecting Newcastle disease virus, avian influenza virus, avian coronavirus and Rosella rosea and an application of the multiplex PCR detection primer group for simultaneously detecting Newcastle disease virus, avian influenza virus, avian coronavirus and Rosella rosea. The formation of a primer dimer or non-specific binding with other amplified fragments is prevented; the GC contents of the upstream primer and the downstream primer are similar, and more than three continuous G or C do not appear at the 3'end. The annealing temperatures of the primers are similar, the length difference of the target genes of the amplified fragments is distinguished through electrophoresis, and the length difference of the corresponding target genes among the primer groups is greater than or equal to 170bp; the kit can be used for quadruple PCR detection of the Newcastle disease virus, the avian influenza virus, the avian coronavirus and the Rosella murine, the simultaneous amplification and detection of the four pathogens are successfully realized, the result is accurate and stable, the identification degree is high, and the repeatability is good.