110 results about "Rhodotorula" patented technology

The invention discloses a plant-microorganism combined uranium pollution treatment method which comprises the following steps: planting seeds of disinfected uranium-tolerant plants into soil to be remediated, and applying a bacterial suspension of uranium-tolerant strains, namely rhodotorula M1, aspergillus M2 and bacillus M3, into the soil to be remediated, so that the uranium enrichment capacity of the uranium-tolerant plants is remarkably improved; and a relatively good effect of repairing the uranium-polluted soil in the medium-temperature climate region is achieved. Uranium-tolerant plants can be harvested manually or mechanically and transferred to a safe place in a centralized manner to be treated, so that uranium-contaminated soil in the medium-temperature climate zone is effectively repaired.

The invention discloses a compound microorganism and application thereof to improvement of saline-alkali soil, and relates to the technical field of agriculture, the compound microorganism is obtained by compounding halophilic bacillus, rhodotorula mucilaginosa, alkali-resistant rhizobium, desulfurization vibrio and siliconizing bacteria, and the excellent saline-alkali soil improvement effect can be achieved. The functional strains cooperate with one another, have complementary advantages, and play a role in multiple aspects such as soil structure improvement, nutrient supply and absorption, salt and alkali relief, microbial community optimization and the like. The compound microbial preparation has an efficient synergistic mechanism, provides an innovative solution for solving the problem of saline-alkali soil, and shows huge potential in the aspects of promoting agricultural green development and ecological protection.

The invention discloses a brewing method of sugar-free soy sauce, which comprises the following steps: S1, respectively moistening bean slices, puffed vital gluten and fried wheat with water, uniformly mixing, cooking, cooling, inoculating a composite strain containing composite aspergillus oryzae, rhizopus, mucor and saccharified aroma-enhancing yeast, and culturing to prepare high-quality soy sauce yeast; s2, adding water into the puffed vital gluten, mixing, and inoculating protease and pullulanase to prepare enzymolysis mash; s3, supplementing water to the enzymolysis mash to prepare an enzymolysis mash mixed solution, mixing the enzymolysis mash mixed solution with soy sauce koji, ventilating, stirring and fermenting, and transferring into a fermentation tank for fermenting; and S4, squeezing the fermented soy sauce mash to obtain the low-sugar soy sauce with low sugar content. S5, inoculating the low-sugar soy sauce into rhodotorula mucilaginosa for secondary fermentation, and sterilizing and refining after fermentation to obtain a finished product of sugar-free soy sauce; the taste is delicious, and the total sugar content is 1t; 0.5 g / 100mL, and amino acid nitrogen is greater than or equal to 1.2 g / 100mL; the product disclosed by the invention meets the '0' sugar soy sauce standard specified by GB 28050-2011. The production of the sugar-free soy sauce can be realized on the basis of the existing equipment of a soy sauce factory.

The invention discloses a group of mitochondrial signal peptides and application thereof, and a group of signal peptides with a mitochondrial targeting function is obtained for the first time by screening endogenous mitochondrial key functional proteins of rhodotorula. The invention further realizes expression of a target gene through mitochondrial signal peptide mediation, realizes expression of a target product synthetic pathway gene in rhodotorula mitochondria, and successfully constructs synthetic pathways of terpene such as limonene, linalool, patchouli alcohol and kaurene, branched chain alcohol such as isobutanol and organic acid such as 3-hydracrylic acid. Further, carbon metabolic flow of pyruvic acid flowing to the target product is dredged, and synthesis of the mitochondrial region indoor target product is finally realized. The characterization of a group of rhodotorula mitochondrial signal peptides disclosed by the invention can realize the synthesis of mitochondrial regional chamber target products, and lays a foundation for the research on the realization of metabolic engineering transformation in subcellular organelles.

The invention discloses algicidal bacteria and application thereof, and relates to the technical field of microorganism application. The algicidal bacterium is classified and named as rhodotorula mucilaginosa, the strain name of the algicidal bacterium is Rhodotorula mucilaginosa TLJ-1, and the preservation number of the algicidal bacterium is GDMCC No: 64271. The algicidal bacterium is applied to treatment of water blooms of cylindropsis, when the algicidal bacterium is applied, an algicidal bacterium solution is added into a water body, the volume ratio of the algicidal bacterium solution to the cylindropsis is (0.75-3): 2, the optimum pH is 7-8, and the temperature is 28 DEG C. According to the present invention, the rhodotorula mucilaginosa TLJ-1 is successfully separated from the plateau lake, i.e., the iso-long lake, and the rhodotorula mucilaginosa TLJ-1 has good algae-lysing activity, particularly has good inhibition and killing effects on cylindrospora, and has good treatment effects on algal bloom caused by water eutrophication.

The invention discloses a yeast mutant strain capable of efficiently utilizing acetic acid to generate grease. The strain is named as rhodotorula mucilaginosa in taxonomy. The strain is adaptively domesticated for 211 days, and the obtained mutant strain can grow in a culture medium containing at most 1.4 M of sodium acetate. Under the condition of sodium acetate stress, compared with a wild strain, the hysteresis period of the mutant strain is reduced by at least 24 hours, and the grease yield is increased by 197%. According to the strategy, the glucose and sodium acetate metabolism rate and the grease generation efficiency are improved, and a technical basis is provided for establishment of a cell factory for producing lipid-based chemicals.

The present invention provides an essence containing chlorella extract and a preparation method thereof. The chlorella extract process comprises the following steps: raw material pulverization; enzymatic hydrolysis with cellulase and trypsin; primary fermentation using a mixture of Bacillus natto and Bacillus pumilus; addition of bamboo leaf extract, corn steep liquor, and sucrose, followed by inoculation with Rhodotorula glutinosus for secondary fermentation to obtain the chlorella extract. The mixed fermentation of multiple strains exhibits a synergistic effect, resulting in greater enrichment of natural products during the fermentation process compared to single-strain fermentation. Furthermore, the present invention provides an essence containing chlorella extract, glycerin, sodium hyaluronate, honey, and water, which exhibits significant moisturizing, antioxidant, and soothing effects.

The invention discloses rhodotorula mucilaginosa PL10 and application thereof in improving the quality of sweet rice wine, and belongs to the technical field of microorganisms, and the preservation number of the rhodotorula mucilaginosa PL10 is CCTCC NO: M20241214. The invention finds that the sweet rice wine cooperatively fermented by the rhodotorula mucilaginosa PL10 and the rhizopus oryzae has the highest sensory evaluation score, the content of delicate flavor and sweet amino acids in each group is the highest, the proportion of the delicate flavor and sweet amino acids in the total amino acid content is higher than 75%, the bitter amino acids are less, the types of ester compounds and aldehyde ketone compounds in each group are the highest, and the content of the sweet rice wine is the highest. Therefore, when the sweet rice wine is fermented by the rhodotorula mucilaginosa PL10, the content of bitter amino acid can be obviously reduced, the content of sweet amino acid and the content of delicious amino acid can be obviously increased, the variety of ester compounds and aldehyde ketone compounds of the sweet rice wine can be obviously increased, and the problems that the commercial sweet rice wine is boring in wine taste and fragrance and insufficient in flavor can be effectively solved. The research result provides reference and theoretical basis for preparation of the Yunnan sweet rice wine.

The invention belongs to the technical field of food and biological fermentation, and particularly relates to a rhodobryum tofu polysaccharide extract as well as a preparation method and application thereof. The preparation method of the rhodobryum tofu polysaccharide extract comprises the following steps: extracting rhodobryum tofu with hot water, filtering, taking filtrate, and concentrating to obtain a rhodobryum tofu aqueous extract; and precipitating the obtained aqueous extract with ethanol, centrifuging, taking the precipitate, dissolving the precipitate in ionized water, then removing protein by adopting a Sevag method to obtain a crude polysaccharide solution, filling the crude polysaccharide solution into a dialysis bag, dialyzing to remove small molecular substances, and freeze-drying to obtain the rhodotorula tofu polysaccharide extract. The polysaccharide functional components are extracted from the red fungus tofu as a raw material, and the polysaccharide content and component differences of the red fungus tofu obtained by different fermentation strains are verified. The application effect of the rhodobryum tofu polysaccharide extract in reducing the content of effective heavy metal Pb in food is verified, and the rhodobryum tofu polysaccharide extract has a good prospect in reducing the bioavailability of the heavy metal in the food, reducing the toxicity of the heavy metal and improving the food safety.

The invention discloses a rhodotorula mucilaginosa strain as well as a fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms. The rhodotorula mucilaginosa BTN-HB-L28 strain is separated and purified from meconopsis in Lijiang of Yunnan, can be used in the field of cosmetics, has the effects of resisting skin oxidation, resisting aging, repairing skin and relieving skin, can be used as an active matter in skin care products, and has practical application value. The fermentation product filtrate of the strain has excellent HAS inhibition ability, and can significantly inhibit the expression of matrix metalloproteinase 3; the fibroblast proliferation can be obviously promoted under various concentrations; the proportion of SA-beta-Gal positive cells can be remarkably reduced under various concentrations, and the anti-aging effect is achieved; the antioxidant composition has remarkable DPPH and ABTS free radical scavenging capacity and has a good antioxidant effect; the secretion amount of cell inflammatory factors NO, IL-6 and TNF-alpha can be remarkably inhibited, and the good relieving effect is achieved.

The invention discloses a cough and asthma relieving composition, and belongs to the field of traditional Chinese medicines. The cough and asthma relieving composition is prepared from traditional Chinese medicine fermented particles, semen armeniacae amarae, folia perillae acutae and radix peucedani, the traditional Chinese medicine fermented particles are prepared from pericarpium citri reticulatae, radix platycodonis and radix glycyrrhizae as raw materials, cellulase is firstly used for conducting enzymolysis on the raw materials to obtain enzymatic hydrolysate, then lactobacillus plantarum, lactobacillus johnsonii and rhodotorula mucilaginosa are used for conducting mixed fermentation on the enzymatic hydrolysate to obtain fermentation liquor, and the fermentation liquor is prepared into the cough and asthma relieving composition. And sterilizing, filtering, drying and granulating the fermentation liquor to obtain the traditional Chinese medicine fermented granules. The traditional Chinese medicine fermented granules provided by the invention are compounded with the semen armeniacae amarae, the folium perillae and the radix peucedani for use, so that a synergistic effect is achieved, and an excellent cough and asthma relieving effect is achieved.

The invention provides a phosphorus release improving type compound fertilizer based on a fungal extracellular polymer, a preparation method and application, and belongs to the technical field of microorganisms, the phosphorus release improving type compound fertilizer is prepared by compounding the extracellular polymer generated by specific rhodotorula mucilaginosa WTSX01 and inorganic phosphate according to the mass ratio of 1: 9; the preparation method comprises the following steps: extracting and purifying high-activity EPS from a strain fermentation broth by a low-temperature dialysis method, separating lipid from EPS as a natural adhesive by a cosolvent extraction method, and finally mixing EPS, lipid and inorganic phosphate. The invention also discloses a synergistic application of the fertilizer and a phosphorus-solubilizing fungus aspergillus niger (CCTCC No. M2023240), so that the release efficiency of phosphorus in soil is improved; the fertilizer provided by the invention provides exclusive nutrition for phosphate-solubilizing microorganisms, effectively overcomes indigenous microorganism competition, remarkably promotes microorganism growth and phosphorus release, and realizes reduction and synergism of phosphate fertilizer and yield increase of crops.

The invention discloses application of rhodotorula red in preparation of a product for effectively preventing / treating hypercholesteremia, and belongs to the technical field of dual purposes of medicine and food. The invention relates to an application of rhodotorula benthamiana in preparation of a product for effectively preventing / treating hypercholesteremia. The rhodotorula benthamiana is rhodotorula benthamiana electrostatic spinning microspheres. Compared with a rhodotorula red bile system, the rhodotorula red electrostatic spinning microsphere system has an obviously better intervention effect on hypercholesterolemia. Meanwhile, compared with a rhodotorula rubra bile system, the rhodotorula rubra electrostatic spinning microsphere system can effectively improve the serum and liver inflammatory factor level of a hypercholesteremia mouse and prevent excessive accumulation of liver lipid, especially ceramide lipid, of the hypercholesteremia mouse.

The invention relates to a rhodotorula mucilaginosa strain and a yellow wine co-fermentation process thereof, and belongs to the technical field of fermentation engineering and strains. The invention provides the rhodotorula mucilaginosa with the fermentation characteristics of low temperature resistance, acid resistance, high yield of beta-glucosidase and aroma production, sequential inoculation is adopted, the rhodotorula mucilaginosa H04 is firstly inoculated for fermentation for two days, then the saccharomyces cerevisiae is inoculated, and the yellow wine co-fermentation is carried out by an equal-proportion 20 DEG C low-temperature fermentation process. The yellow rice wine meeting national standard requirements of yellow rice wine can be obtained after 20 days of fermentation, and the volatile flavor type, content, taste and aroma sensory quality of the yellow rice wine after fermentation are improved.

The invention discloses a preparation method of a microbial complex microbial inoculant and a Chinese herbal medicine complex microbial inoculant and application of the microbial complex microbial inoculant in offshore fish culture, and belongs to the technical field of microbial engineering. The composite microbial inoculant comprises bacillus megatherium and rhodotorula mucilaginosa. In addition, the invention also provides a preparation method of the Chinese herbal medicine complex microbial inoculant, which comprises the following steps: mixing the microbial complex microbial inoculant, the Chinese herbal medicine and the base solution, and fermenting. In addition, the invention also provides a Chinese herbal medicine complex microbial inoculant prepared by the preparation method. In addition, the invention also provides a feed, and the mass addition amount of the compound microbial inoculant prepared by the preparation method in the feed is 5%-10%. In addition, the invention also provides an application of the Chinese herbal medicine complex microbial inoculant prepared by the preparation method in offshore fish culture. The microbial complex microbial inoculant can be used for Chinese herbal medicine fermentation, and the problem of obesity of fishes caused by artificial sweeteners can be effectively solved after the fishes are fed with the microbial complex microbial inoculant.

The invention belongs to the technical field of microbial fermentation, and particularly relates to rhodotorula mucilaginosa JQRM001 and application thereof. The invention provides a rhodotorula mucilaginosa JQRM001, the Latin name of the rhodotorula mucilaginosa JQRM001 is Rhodotorula mucilaginosa, the rhodotorula mucilaginosa JQRM001 is preserved in China General Microbiological Culture Collection Center, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 15, 2025, and the preservation number is CGMCC No.35240. The invention further provides a preparation method of the rhodotorula mucilaginosa. The rhodotorula mucilaginosa JQRM001 not only has excellent performance of high temperature resistance and acid resistance, but also can be used for specifically synthesizing alkylpyrazine flavor substances and remarkably improving the antioxidant activity of plant polysaccharides.

The invention discloses a peroxisome positioning signal peptide and application thereof. The invention relates to an application of an N-terminal II-type peroxisome positioning signal peptide alone or in combination with a C-terminal I-type signal peptide in the biosynthesis of a compartment high-value compound. The invention comprises the construction of an auxiliary carrier of two peroxisome signal peptides, and covers the application of the auxiliary carrier in the biosynthesis of terpene substances such as limonene, linalool and patchouli alcohol, and flavonoid substances such as daidzein, genistein, 3-hydracrylic acid and coenzyme Q10. The peroxisome signal peptide disclosed by the invention can provide a new element for metabolic engineering modification of subcellular organelles of rhodotorula expansa.

The invention belongs to the technical field of sewage treatment, and particularly relates to an urban domestic sewage treatment method. Chitosan, modified activated carbon and a complex microbial inoculant formed by compounding any two strains of spirulina, rhodotorula mucilaginosa and micrococcus luteus are specifically combined, the chitosan in the composition serves as a carrier of the complex microbial inoculant, nutrients can be provided for survival of the complex microbial inoculant, and the survival time of the microbial inoculant is prolonged; the modified activated carbon has high adsorption capacity on the complex microbial inoculant, so that slow release of the complex microbial inoculant in the later period is facilitated; therefore, the composition provided by the invention can be used for purifying the urban domestic sewage, the purpose of purifying the urban domestic sewage is achieved, ecologicalization and recycling of domestic sewage treatment are realized, and a new thought is provided for urban sewage treatment.

The invention discloses Rhodosporidium toruloides for aquatic feed and application of the Rhodosporidium toruloides to aquaculture, relates to the technical field of microorganisms, and particularly relates to Rhodosporidium toruloides Ho7-3 which can be applied to aquaculture, has the effects of improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system, and can be used for improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system. The growth performance of cultured fishes can be obviously improved.

A strain of Acanthopanax senticosus endophytic fungus HASZ2 producing isofraquinone and protocatechuic acid and its application, relating to the field of microorganisms, the Acanthopanax senticosus endophytic fungus HASZ2 is Rhodotorula glutinosus HASZ2, which has been preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, the preservation date is May 16, 2024, and the preservation number is CCTCC NO: M2024973. The strain has good antioxidant activity. After secondary metabolite analysis of its fermentation broth, it was found that the strain can produce isofraquinone and protocatechuic acid. The present invention is used for anti-oxidation and production of Acanthopanax senticosus secondary metabolites.

The invention relates to the technical field of microorganisms, in particular to a method for producing astaxanthin emulsion from phaffia rhodozyma, which specifically comprises the following steps of: performing superfine grinding on dried phaffia rhodozyma, extracting astaxanthin by using a solvent, dissolving the astaxanthin in soybean oil, taking whey protein as a water phase, preparing O / W emulsion, adding tannic acid to protect the astaxanthin from being influenced by enzymatic crosslinking, and preparing the astaxanthin emulsion by using the tannic acid. And a'multilayer 'interfacial film is formed through electrostatic assembly and enzymatic crosslinking. The chitosan-sodium alginate'multi-layer 'interfacial film improves the mechanical strength of the interfacial film, and limits the oxidizing agent from permeating into the internal oil phase to be in contact with the astaxanthin. Flocculation of the astaxanthin emulsion is inhibited through the charge neutralization effect of sodium alginate grafted polyethylene glycol sheltered salt ions, enzymatic crosslinking is conducted on chitosan grafted peptide fragments, and targeted release of the astaxanthin in the intestinal tract is achieved. The use amount of tyrosinase and the enzymatic reaction time are controlled, the residue of tyrosinase with oxidizing property is prevented, and tannic acid prevents the oxidizing substance generated by the reaction of tyrosinase from oxidizing astaxanthin.

The present invention discloses a red yeast strain M1 and its applications. The strain has strong heavy metal tolerance and can remove convertible uranium from soil. Using the strain M1 for in-situ remediation of uranium-contaminated soil is environmentally friendly, free of drug residues, and highly biosafe, significantly reducing remediation costs and minimizing soil structural damage.

This invention relates to a high-yield coenzyme Q method. 10 The establishment and application of *Rhodotorula globulinii* strains. Compared to rational metabolic engineering, the coenzyme Q of this invention... 10 High-yielding Rhodopseudomonas aeruginosa bacteria were used as a template by the global regulatory factor prrA gene. Through directed evolution, different mutant strains were constructed, and combined with high-throughput screening methods, coenzyme Q was achieved. 10 Increased yield. This invention starts from global regulatory factors and leverages complex intracellular regulatory mechanisms to achieve fine-grained global regulation under minute genomic perturbations, providing a new approach for the engineering modification of strains.

The invention discloses a gastrointestinal delivery carrier of a high-activity rhodotorula compound and application of the gastrointestinal delivery carrier in colon fermentation, and belongs to the field of research of food science, pharmaceutical preparations, nutriology and microbiology. The invention explores the storage stability and digestive tract metabolism conditions of the rhodotorula high-activity compound under different carrier forms, namely corn oil (F-oil) loaded with the rhodotorula high-activity compound, corn oil nano-emulsion spray drying powder (F-EP) of the rhodotorula high-activity compound and corn oil nano-emulsion alginate gel (F-EPA) of the rhodotorula high-activity compound. Wherein the storage stability of the F-EPA is optimal. The three carriers do not release the high-activity rhodotorula compound in the in-vitro oral cavity and stomach stages, but in the small intestine stage, the digestibility and the biological accessibility of F-EP are the highest, and the release of F-EPA is the minimum. The F-EPA can effectively promote the rhodotorula red pigment to regulate the structural composition and abundance value of intestinal microbiome.

The invention provides a rhodotorula diovalvata strain and application of the rhodotorula diovalvata strain. The preservation number of the rhodotorula diobovata is CGMCC (China General Microbiological Culture Collection Center) NO.32322. The rhodotorula diobovata disclosed by the invention has the advantages that the preservation number is CGMCC NO.32322 The rhodotorula difformis is applied to the enhanced fermentation of the tobacco leaves, and compared with a control group fermented by sterile water, the rhodotorula difformis fermentation group can obviously improve the content of chlorophyll degradation products, carotenoid degradation products, cembrane degradation products and other aroma components. The results show that the application of the rhodotorula difformis not only enhances the aroma characteristic of the tobacco leaves, but also contributes to improving the overall quality of the tobacco leaves, and provides an effective biotechnological means for a tobacco fermentation process.

The invention belongs to the technical field of biology, and particularly relates to a method for producing enzyme by using apple pomace and molasses and application. The method for preparing the apple pomace enzyme comprises the following steps: adding water into apple pomace and pulping; the preparation method comprises the following steps: adding molasses, uniformly stirring, sterilizing at high temperature, cooling, inoculating a mixed bacterial agent comprising lactobacillus plantarum (CICC21801), fermented lactobacillus mucus (CICC21835), fermented pichia pastoris (CICC33372) and rhodotorula mucilaginosa (CICC31192), and fermenting at 25-35 DEG C for 5-15 days. The apple pomace enzyme can be obtained after fermentation is finished. The detection result shows that the plant active ingredients of the apple pomace ferment are increased, the apple pomace ferment can improve soil and promote plants to absorb nutrient substances, the plant yield is increased by 22.10%, and the utilization value of the apple pomace and molasses is increased.

The application discloses a kind of biological inoculums for improving fermentation environment of waste pool of waste incineration plant, solve high kitchen garbage heavy metal pollution problem.It uses "functional strain + kitchen waste oil modified biochar carrier" collaborative design, functional strain includes Yarrowia lipolytica, Aspergillus oryzae, salt-tolerant lactic acid bacteria and Rhodotorula, Rhodotorula can efficiently passivate Pb 2+ , Cd 2+ , strain synergistic degradation of oil and starch.Application of percolate soluble heavy metal content reaches standard, reduces heavy metal treatment reagent dosage 25%-30%, reduces fly ash "hazardous waste" disposal ratio, realizes fermentation improvement and heavy metal passivation integration, with environmental protection and economic benefits.

The application discloses a gastrointestinal delivery carrier of high-activity compound of Rhodotorula and application thereof in colon fermentation, and belongs to the field of food science, pharmaceutical preparation, nutrition and microbiology research. The application explores the storage stability and digestive tract metabolism of the high-activity compound of Rhodotorula in different carrier forms, namely corn oil loaded with the high-activity compound of Rhodotorula (F-oil), spray-dried powder of corn oil nanoemulsion of the high-activity compound of Rhodotorula (F-EP) and alginate gel of corn oil nanoemulsion of the high-activity compound of Rhodotorula (F-EPA), wherein the storage stability of F-EPA is the best. The three carriers in the application do not release the high-activity compound of Rhodotorula in the oral cavity and stomach stages in vitro, but in the small intestine stage, the digestion rate and bioavailability of F-EP are the highest, and the release of F-EPA is the least. F-EPA can effectively promote the regulation of intestinal microbial community structure and abundance value by Rhodotorula rubin.

This invention discloses a glucose phosphate mutase gene Rk PGM Its nucleotide sequence is shown in SEQ ID NO:1, and the amino acid sequence encoded by this gene is shown in SEQ ID NO:2; this gene was isolated from *Rhodotorula rubrum* (…). Rhodosporidium kratochvilovae YM25235 was used to ligate the gene to a vector and transfected into Rhodotorula rubrum cells. Experimental results showed that overexpression of Rk... PGM The gene will increase the level of extracellular polysaccharide synthesis in this strain; this invention modifies microorganisms through genetic engineering to improve their ability to secrete extracellular polysaccharides, providing a theoretical basis for increasing the yield of extracellular polysaccharides in Rhodotorula rubrum.

This invention relates to the field of genetic engineering technology and provides a method for increasing erythromycin yield by modifying the SACE_5680 gene of *Rhodotorula sacchariformis*. The method involves deleting the GntR family SACE_5680 gene in *Rhodotorula sacchariformis* through genetic engineering, thereby obtaining a high-yielding engineered strain of *Rhodotorula sacchariformis* for erythromycin production. Erythromycin is then produced by fermentation using this high-yielding engineered strain. The nucleotide sequence of the SACE_5680 gene is shown in SEQ ID NO. 1. This invention also provides an application of the above method. In this study, the negative regulator of erythromycin biosynthesis, SACE_5680, was screened. By deleting a copy of the SACE_5680 gene on the chromosome of *Rhodotorula sacchariformis* through genetic engineering, a high-yielding strain of erythromycin can be obtained, providing technical support for increasing erythromycin fermentation yield in industrial production.