89 results about "Rhodotorula" patented technology

The invention discloses a plant-microorganism combined uranium pollution treatment method which comprises the following steps: planting seeds of disinfected uranium-tolerant plants into soil to be remediated, and applying a bacterial suspension of uranium-tolerant strains, namely rhodotorula M1, aspergillus M2 and bacillus M3, into the soil to be remediated, so that the uranium enrichment capacity of the uranium-tolerant plants is remarkably improved; and a relatively good effect of repairing the uranium-polluted soil in the medium-temperature climate region is achieved. Uranium-tolerant plants can be harvested manually or mechanically and transferred to a safe place in a centralized manner to be treated, so that uranium-contaminated soil in the medium-temperature climate zone is effectively repaired.

The invention discloses a compound microorganism and application thereof to improvement of saline-alkali soil, and relates to the technical field of agriculture, the compound microorganism is obtained by compounding halophilic bacillus, rhodotorula mucilaginosa, alkali-resistant rhizobium, desulfurization vibrio and siliconizing bacteria, and the excellent saline-alkali soil improvement effect can be achieved. The functional strains cooperate with one another, have complementary advantages, and play a role in multiple aspects such as soil structure improvement, nutrient supply and absorption, salt and alkali relief, microbial community optimization and the like. The compound microbial preparation has an efficient synergistic mechanism, provides an innovative solution for solving the problem of saline-alkali soil, and shows huge potential in the aspects of promoting agricultural green development and ecological protection.

The present invention provides an essence containing chlorella extract and a preparation method thereof. The chlorella extract process comprises the following steps: raw material pulverization; enzymatic hydrolysis with cellulase and trypsin; primary fermentation using a mixture of Bacillus natto and Bacillus pumilus; addition of bamboo leaf extract, corn steep liquor, and sucrose, followed by inoculation with Rhodotorula glutinosus for secondary fermentation to obtain the chlorella extract. The mixed fermentation of multiple strains exhibits a synergistic effect, resulting in greater enrichment of natural products during the fermentation process compared to single-strain fermentation. Furthermore, the present invention provides an essence containing chlorella extract, glycerin, sodium hyaluronate, honey, and water, which exhibits significant moisturizing, antioxidant, and soothing effects.

The invention belongs to the technical field of food and biological fermentation, and particularly relates to a rhodobryum tofu polysaccharide extract as well as a preparation method and application thereof. The preparation method of the rhodobryum tofu polysaccharide extract comprises the following steps: extracting rhodobryum tofu with hot water, filtering, taking filtrate, and concentrating to obtain a rhodobryum tofu aqueous extract; and precipitating the obtained aqueous extract with ethanol, centrifuging, taking the precipitate, dissolving the precipitate in ionized water, then removing protein by adopting a Sevag method to obtain a crude polysaccharide solution, filling the crude polysaccharide solution into a dialysis bag, dialyzing to remove small molecular substances, and freeze-drying to obtain the rhodotorula tofu polysaccharide extract. The polysaccharide functional components are extracted from the red fungus tofu as a raw material, and the polysaccharide content and component differences of the red fungus tofu obtained by different fermentation strains are verified. The application effect of the rhodobryum tofu polysaccharide extract in reducing the content of effective heavy metal Pb in food is verified, and the rhodobryum tofu polysaccharide extract has a good prospect in reducing the bioavailability of the heavy metal in the food, reducing the toxicity of the heavy metal and improving the food safety.

The invention discloses a rhodotorula mucilaginosa strain as well as a fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms. The rhodotorula mucilaginosa BTN-HB-L28 strain is separated and purified from meconopsis in Lijiang of Yunnan, can be used in the field of cosmetics, has the effects of resisting skin oxidation, resisting aging, repairing skin and relieving skin, can be used as an active matter in skin care products, and has practical application value. The fermentation product filtrate of the strain has excellent HAS inhibition ability, and can significantly inhibit the expression of matrix metalloproteinase 3; the fibroblast proliferation can be obviously promoted under various concentrations; the proportion of SA-beta-Gal positive cells can be remarkably reduced under various concentrations, and the anti-aging effect is achieved; the antioxidant composition has remarkable DPPH and ABTS free radical scavenging capacity and has a good antioxidant effect; the secretion amount of cell inflammatory factors NO, IL-6 and TNF-alpha can be remarkably inhibited, and the good relieving effect is achieved.

The invention provides a phosphorus release improving type compound fertilizer based on a fungal extracellular polymer, a preparation method and application, and belongs to the technical field of microorganisms, the phosphorus release improving type compound fertilizer is prepared by compounding the extracellular polymer generated by specific rhodotorula mucilaginosa WTSX01 and inorganic phosphate according to the mass ratio of 1: 9; the preparation method comprises the following steps: extracting and purifying high-activity EPS from a strain fermentation broth by a low-temperature dialysis method, separating lipid from EPS as a natural adhesive by a cosolvent extraction method, and finally mixing EPS, lipid and inorganic phosphate. The invention also discloses a synergistic application of the fertilizer and a phosphorus-solubilizing fungus aspergillus niger (CCTCC No. M2023240), so that the release efficiency of phosphorus in soil is improved; the fertilizer provided by the invention provides exclusive nutrition for phosphate-solubilizing microorganisms, effectively overcomes indigenous microorganism competition, remarkably promotes microorganism growth and phosphorus release, and realizes reduction and synergism of phosphate fertilizer and yield increase of crops.

The invention relates to a rhodotorula mucilaginosa strain and a yellow wine co-fermentation process thereof, and belongs to the technical field of fermentation engineering and strains. The invention provides the rhodotorula mucilaginosa with the fermentation characteristics of low temperature resistance, acid resistance, high yield of beta-glucosidase and aroma production, sequential inoculation is adopted, the rhodotorula mucilaginosa H04 is firstly inoculated for fermentation for two days, then the saccharomyces cerevisiae is inoculated, and the yellow wine co-fermentation is carried out by an equal-proportion 20 DEG C low-temperature fermentation process. The yellow rice wine meeting national standard requirements of yellow rice wine can be obtained after 20 days of fermentation, and the volatile flavor type, content, taste and aroma sensory quality of the yellow rice wine after fermentation are improved.

The invention discloses a preparation method of a microbial complex microbial inoculant and a Chinese herbal medicine complex microbial inoculant and application of the microbial complex microbial inoculant in offshore fish culture, and belongs to the technical field of microbial engineering. The composite microbial inoculant comprises bacillus megatherium and rhodotorula mucilaginosa. In addition, the invention also provides a preparation method of the Chinese herbal medicine complex microbial inoculant, which comprises the following steps: mixing the microbial complex microbial inoculant, the Chinese herbal medicine and the base solution, and fermenting. In addition, the invention also provides a Chinese herbal medicine complex microbial inoculant prepared by the preparation method. In addition, the invention also provides a feed, and the mass addition amount of the compound microbial inoculant prepared by the preparation method in the feed is 5%-10%. In addition, the invention also provides an application of the Chinese herbal medicine complex microbial inoculant prepared by the preparation method in offshore fish culture. The microbial complex microbial inoculant can be used for Chinese herbal medicine fermentation, and the problem of obesity of fishes caused by artificial sweeteners can be effectively solved after the fishes are fed with the microbial complex microbial inoculant.

The invention belongs to the technical field of microbial fermentation, and particularly relates to rhodotorula mucilaginosa JQRM001 and application thereof. The invention provides a rhodotorula mucilaginosa JQRM001, the Latin name of the rhodotorula mucilaginosa JQRM001 is Rhodotorula mucilaginosa, the rhodotorula mucilaginosa JQRM001 is preserved in China General Microbiological Culture Collection Center, the address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 15, 2025, and the preservation number is CGMCC No.35240. The invention further provides a preparation method of the rhodotorula mucilaginosa. The rhodotorula mucilaginosa JQRM001 not only has excellent performance of high temperature resistance and acid resistance, but also can be used for specifically synthesizing alkylpyrazine flavor substances and remarkably improving the antioxidant activity of plant polysaccharides.

The invention discloses a peroxisome positioning signal peptide and application thereof. The invention relates to an application of an N-terminal II-type peroxisome positioning signal peptide alone or in combination with a C-terminal I-type signal peptide in the biosynthesis of a compartment high-value compound. The invention comprises the construction of an auxiliary carrier of two peroxisome signal peptides, and covers the application of the auxiliary carrier in the biosynthesis of terpene substances such as limonene, linalool and patchouli alcohol, and flavonoid substances such as daidzein, genistein, 3-hydracrylic acid and coenzyme Q10. The peroxisome signal peptide disclosed by the invention can provide a new element for metabolic engineering modification of subcellular organelles of rhodotorula expansa.

The invention belongs to the technical field of sewage treatment, and particularly relates to an urban domestic sewage treatment method. Chitosan, modified activated carbon and a complex microbial inoculant formed by compounding any two strains of spirulina, rhodotorula mucilaginosa and micrococcus luteus are specifically combined, the chitosan in the composition serves as a carrier of the complex microbial inoculant, nutrients can be provided for survival of the complex microbial inoculant, and the survival time of the microbial inoculant is prolonged; the modified activated carbon has high adsorption capacity on the complex microbial inoculant, so that slow release of the complex microbial inoculant in the later period is facilitated; therefore, the composition provided by the invention can be used for purifying the urban domestic sewage, the purpose of purifying the urban domestic sewage is achieved, ecologicalization and recycling of domestic sewage treatment are realized, and a new thought is provided for urban sewage treatment.

The invention discloses Rhodosporidium toruloides for aquatic feed and application of the Rhodosporidium toruloides to aquaculture, relates to the technical field of microorganisms, and particularly relates to Rhodosporidium toruloides Ho7-3 which can be applied to aquaculture, has the effects of improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system, and can be used for improving fish intestinal digestive enzyme activity, immune response and an anti-oxidation defense system. The growth performance of cultured fishes can be obviously improved.

The invention relates to the technical field of microorganisms, in particular to a method for producing astaxanthin emulsion from phaffia rhodozyma, which specifically comprises the following steps of: performing superfine grinding on dried phaffia rhodozyma, extracting astaxanthin by using a solvent, dissolving the astaxanthin in soybean oil, taking whey protein as a water phase, preparing O / W emulsion, adding tannic acid to protect the astaxanthin from being influenced by enzymatic crosslinking, and preparing the astaxanthin emulsion by using the tannic acid. And a'multilayer 'interfacial film is formed through electrostatic assembly and enzymatic crosslinking. The chitosan-sodium alginate'multi-layer 'interfacial film improves the mechanical strength of the interfacial film, and limits the oxidizing agent from permeating into the internal oil phase to be in contact with the astaxanthin. Flocculation of the astaxanthin emulsion is inhibited through the charge neutralization effect of sodium alginate grafted polyethylene glycol sheltered salt ions, enzymatic crosslinking is conducted on chitosan grafted peptide fragments, and targeted release of the astaxanthin in the intestinal tract is achieved. The use amount of tyrosinase and the enzymatic reaction time are controlled, the residue of tyrosinase with oxidizing property is prevented, and tannic acid prevents the oxidizing substance generated by the reaction of tyrosinase from oxidizing astaxanthin.

The present invention discloses a red yeast strain M1 and its applications. The strain has strong heavy metal tolerance and can remove convertible uranium from soil. Using the strain M1 for in-situ remediation of uranium-contaminated soil is environmentally friendly, free of drug residues, and highly biosafe, significantly reducing remediation costs and minimizing soil structural damage.

This invention relates to a high-yield coenzyme Q method. 10 The establishment and application of *Rhodotorula globulinii* strains. Compared to rational metabolic engineering, the coenzyme Q of this invention... 10 High-yielding Rhodopseudomonas aeruginosa bacteria were used as a template by the global regulatory factor prrA gene. Through directed evolution, different mutant strains were constructed, and combined with high-throughput screening methods, coenzyme Q was achieved. 10 Increased yield. This invention starts from global regulatory factors and leverages complex intracellular regulatory mechanisms to achieve fine-grained global regulation under minute genomic perturbations, providing a new approach for the engineering modification of strains.

The invention provides a rhodotorula diovalvata strain and application of the rhodotorula diovalvata strain. The preservation number of the rhodotorula diobovata is CGMCC (China General Microbiological Culture Collection Center) NO.32322. The rhodotorula diobovata disclosed by the invention has the advantages that the preservation number is CGMCC NO.32322 The rhodotorula difformis is applied to the enhanced fermentation of the tobacco leaves, and compared with a control group fermented by sterile water, the rhodotorula difformis fermentation group can obviously improve the content of chlorophyll degradation products, carotenoid degradation products, cembrane degradation products and other aroma components. The results show that the application of the rhodotorula difformis not only enhances the aroma characteristic of the tobacco leaves, but also contributes to improving the overall quality of the tobacco leaves, and provides an effective biotechnological means for a tobacco fermentation process.

The application discloses a kind of biological inoculums for improving fermentation environment of waste pool of waste incineration plant, solve high kitchen garbage heavy metal pollution problem.It uses "functional strain + kitchen waste oil modified biochar carrier" collaborative design, functional strain includes Yarrowia lipolytica, Aspergillus oryzae, salt-tolerant lactic acid bacteria and Rhodotorula, Rhodotorula can efficiently passivate Pb 2+ , Cd 2+ , strain synergistic degradation of oil and starch.Application of percolate soluble heavy metal content reaches standard, reduces heavy metal treatment reagent dosage 25%-30%, reduces fly ash "hazardous waste" disposal ratio, realizes fermentation improvement and heavy metal passivation integration, with environmental protection and economic benefits.

The application discloses a gastrointestinal delivery carrier of high-activity compound of Rhodotorula and application thereof in colon fermentation, and belongs to the field of food science, pharmaceutical preparation, nutrition and microbiology research. The application explores the storage stability and digestive tract metabolism of the high-activity compound of Rhodotorula in different carrier forms, namely corn oil loaded with the high-activity compound of Rhodotorula (F-oil), spray-dried powder of corn oil nanoemulsion of the high-activity compound of Rhodotorula (F-EP) and alginate gel of corn oil nanoemulsion of the high-activity compound of Rhodotorula (F-EPA), wherein the storage stability of F-EPA is the best. The three carriers in the application do not release the high-activity compound of Rhodotorula in the oral cavity and stomach stages in vitro, but in the small intestine stage, the digestion rate and bioavailability of F-EP are the highest, and the release of F-EPA is the least. F-EPA can effectively promote the regulation of intestinal microbial community structure and abundance value by Rhodotorula rubin.

This invention discloses a glucose phosphate mutase gene Rk PGM Its nucleotide sequence is shown in SEQ ID NO:1, and the amino acid sequence encoded by this gene is shown in SEQ ID NO:2; this gene was isolated from *Rhodotorula rubrum* (…). Rhodosporidium kratochvilovae YM25235 was used to ligate the gene to a vector and transfected into Rhodotorula rubrum cells. Experimental results showed that overexpression of Rk... PGM The gene will increase the level of extracellular polysaccharide synthesis in this strain; this invention modifies microorganisms through genetic engineering to improve their ability to secrete extracellular polysaccharides, providing a theoretical basis for increasing the yield of extracellular polysaccharides in Rhodotorula rubrum.

This invention relates to the field of genetic engineering technology and provides a method for increasing erythromycin yield by modifying the SACE_5680 gene of *Rhodotorula sacchariformis*. The method involves deleting the GntR family SACE_5680 gene in *Rhodotorula sacchariformis* through genetic engineering, thereby obtaining a high-yielding engineered strain of *Rhodotorula sacchariformis* for erythromycin production. Erythromycin is then produced by fermentation using this high-yielding engineered strain. The nucleotide sequence of the SACE_5680 gene is shown in SEQ ID NO. 1. This invention also provides an application of the above method. In this study, the negative regulator of erythromycin biosynthesis, SACE_5680, was screened. By deleting a copy of the SACE_5680 gene on the chromosome of *Rhodotorula sacchariformis* through genetic engineering, a high-yielding strain of erythromycin can be obtained, providing technical support for increasing erythromycin fermentation yield in industrial production.

The invention discloses rhodotorula glutinis LUCNOVA 002 as well as a zymophyte agent and application thereof. The preservation number of the rhodotorula glutinis LUCNOVA 002 is CCTCC (China Center for Type Culture Collection) NO: M2022812. The fermentation bacterial agent is prepared from a combination of rhodotorula glutinis LUCNOVA 002 and saccharomyces cerevisiae NJ-LK12. According to the invention, the rhodotorula glutinis LUCNOVA 002 is obtained through screening, a liquid-solid combined deep fermentation technology is fully utilized, a fermentation inoculant composed of composite seed liquid of rhodotorula glutinis and saccharomyces cerevisiae is used for fermentation of the flaxseed-soybean meal compound, the production raw materials are sufficient, the production process is simple, the production cost is low, and the flaxseed-soybean meal compound is convenient to store and use.

The invention discloses an agent for intensively repairing lead pollution, a preparation method of the agent and a repairing method. The agent is prepared from 15%-20% of penicillium chrysogenum, 10%-15% of rhodotorula glutinis, 25%-30% of hydroxyapatite, 5%-8% of sodium alginate, 10%-15% of modified crab shell powder and the balance water. The preparation method comprises the steps of S1, pretreatment, S2, medicament compounding and forming and S3, curing treatment. The reinforced lead pollution remediation agent prepared by the invention can be used for completing remediation of contaminated soil within 7 days, so that the concentration of Pb < 2 + > is reduced by 93% or above; and after 6 months of natural aging, the conversion rate of the fixed-state lead is less than 5%, and the lead repairing agent has efficient lead repairing capability and repairing long-term effect.

According to the marine rhodotorula mucilaginosa and the method for mixed inoculation of the marine rhodotorula mucilaginosa and the lactobacillus rhamnosus, the selected marine rhodotorula mucilaginosa can efficiently degrade NDMA in air-dried sausages and smelly mandarin fish, various nitrosamines in fermented meat products can be jointly degraded through the synergistic effect of the marine rhodotorula mucilaginosa and the NDMA in the smelly mandarin fish, in addition, the mixed bacteria synergistically show an excellent degradation effect on biogenic amines, and the marine rhodotorula and the degradation efficiency is far higher than that of single-bacterium fermentation. In the invention, the combination of adaptive directed evolution and mutation breeding is used for improving the nitrosamine degradation capability of the strain for the first time, the marine rhodotorula mucilaginosa SY-AN-9 obtained by the technology is applied to degradation of nitrosamine in fermented meat products for the first time, and a method for synergistically degrading biogenic amine and nitrosamine by mixed bacteria is developed. The safety of the fermented meat product is improved, the application range of the marine rhodotorula mucilaginosa and the rhamnosus lactobacillus is widened, and the application of the marine rhodotorula mucilaginosa and the rhamnosus lactobacillus in the food industry is promoted.

The invention relates to Rhodotorula paludi and an application of the Rhodotorula paludi in aroma enhancement and quality improvement of a tobacco stem concentrated solution, the Rhodotorula paludi is named as a Rhodotorula paludi H72 strain, the preservation number is CGMCC No.34875, and the preservation date is June 12, 2025. The Rhodotorula paludi is named as Rhodotorula paludi H72 strain, the preservation number is CGMCC No.34875, and the preservation number is CGMCC No.34875. According to the invention, a new Rhodotorula paludigena strain is separated from cigar tobacco leaves and is preserved, the new Rhodotorula paludigena strain is named as Rhodotorula paludigena strain H72, and the strain can significantly improve the relative content of aroma substances in the tobacco stem concentrated solution and increase new aroma substances, significantly improve the sensory quality of the tobacco stem concentrated solution, and improve the quality of the tobacco stem concentrated solution. The strain or the microbial inoculum can be used for preparing tobacco products after fermenting a tobacco stem concentrated solution or a diluent thereof, so that the aroma and the quality of the tobacco products are improved.

Capsule structure (C) with a red yeast compound, comprising: - a capsule body (10) comprising a first capsule shell (11) and a second capsule shell (12), wherein the first capsule shell (11) and the second capsule shell (12) are placed on top of each other in such a way that they together enclose a closed receiving space (13); and - several functional particles (20) arranged in the receiving space (13) of the capsule body (10), wherein the composition of the functional particles (20) includes red yeast (21), nattokinase (22) and coenzyme Q10 (23).

The invention belongs to the technical field of tobacco strains, and particularly relates to a rhodotorula mucilaginosa H04 strain and application thereof in preparation of tobacco flavor. The rhodotorula mucilaginosa H04 provided by the invention has excellent aroma producing performance and can produce tobacco characteristic aroma substances such as alcohols, aldehydes, esters and pyrazines at high yield. The rhodotorula mucilaginosa H04 has good tolerance to nicotine, can still keep high growth activity under the condition that the nicotine concentration is as high as 8 mg / mL, and is suitable for being used for preparing tobacco flavor; the tobacco flavor prepared by the invention contains a plurality of natural aroma components, has good coordination with the original aroma of tobacco, and can significantly improve the aroma richness and comfort of tobacco products; the highest beta-glucosidase activity of the rhodotorula mucilaginosa H04 reaches 464.0 + / -24.4 U / mL, and the laccase activity reaches 60.4 + / -3.2 U / mL, so that the release of bound aroma precursor substances in tobacco is facilitated, and the aroma expression is enhanced.

The application discloses a furaldehyde-tolerant Rhodotorula mucilaginosa strain and application thereof, the strain is preserved in the China General Microbiological Culture Collection Center, the preservation date is July 1, 2024, the preservation number is CGMCC NO.31122, and the Rhodotorula mucilaginosa strain is named Rhodotorula mucilaginosa F3.The furaldehyde concentration that can be tolerated by the Rhodotorula mucilaginosa F3 strain is 40mM-45mM, so that the production cost of carotenoids can be reduced, the utilization efficiency of lignocellulose hydrolysate is improved, waste utilization is realized, and good commercial and scientific research values are obtained.

The invention discloses rhodotorula mucilaginosa TGy002 and application thereof in enrichment and selenium conversion at low temperature, and belongs to the technical field of microorganisms, the collection number of the rhodotorula mucilaginosa TGy002 is CCTCC NO: M 20251010, the collection site is China Center for Type Culture Collection, and the collection date is May 9, 2025. The rhodotorula mucilaginosa strain TGy002 developed by the invention can maintain the activity of thalli and the stability of metabolites under a low-temperature condition, improves the product quality and stability, not only can solve the problem of non-uniform distribution of selenium resources, but also can provide a safe and efficient organic selenium source for cold regions, and has a wide application prospect.

The invention discloses Chryseobacterium sp. HNK6, the preservation number of the Chryseobacterium sp. HNK6 in Guangdong Microbial Culture Collection Center is GDMCC No: 66436, when the Chryseobacterium sp. HNK6 is applied to degradation of chicken feather, the chicken feather can be degraded at the pH of 7-10 and the temperature of 30-40 DEG C, and the degradation rate is 80% or above; the degradation liquid of the Chryseobacterium HNK6 for degrading chicken feathers is used for culturing rhodotorula glutinis, so that the rhodotorula glutinis can efficiently produce the rhodotorula rubra, the production cost of the rhodotorula rubra is reduced, a new way is provided for industrial production of the rhodotorula rubra, and the rhodotorula rubra HNK6 is suitable for industrial production and market popularization and application.

The invention relates to a compound microbial agent and application thereof in repairing or managing and controlling petroleum contaminated sites, the compound microbial agent comprises compound microorganisms, and the compound microorganisms comprise pseudomonas, Borsella, Rhodotorula abattocida and Chishui Taibai mountain fungi with the viable count ratio of (0.5-20): (0.5-10): (1-2.5): 1. According to the method, the remediation or management and control efficiency of the petroleum contaminated site is improved, the method is particularly suitable for green and efficient remediation and risk management and control of soil and underground water of the powder clayed soil site, and the method has the advantages of being low in cost, convenient to operate and the like and thus has important application prospects.