240 results about "Patchouli" patented technology

The invention discloses production of terpenoids and a strain, and belongs to the technical field of bioengineering. The serratia marcescens engineering bacteria for producing the semi-terpenoids or monoterpenoids are constructed, and can be used for producing linalool, isoprene, isopentenol, 1, 3, 4-triterpenes and 1, 3, 4-triterpenes. The composition is prepared from 1, 8-cineole, alpha-pinene, pinene, gamma-terpinene, geraniol, (+)-limonene, (-)-limonene, myrcene, beta-ocimene, sabinene, (-)-alpha-bisabolol, (-)-alpha-bisabolol, longifolene, wallenenes, beta-elemene, farnesene, patchouli alcohol, acyclic terpene and alpha-santalene. In a 30L fermentation tank, the yield of linalool produced by the serratia engineering bacteria is 40.72 g.L <-1 >.

The invention discloses a group of mitochondrial signal peptides and application thereof, and a group of signal peptides with a mitochondrial targeting function is obtained for the first time by screening endogenous mitochondrial key functional proteins of rhodotorula. The invention further realizes expression of a target gene through mitochondrial signal peptide mediation, realizes expression of a target product synthetic pathway gene in rhodotorula mitochondria, and successfully constructs synthetic pathways of terpene such as limonene, linalool, patchouli alcohol and kaurene, branched chain alcohol such as isobutanol and organic acid such as 3-hydracrylic acid. Further, carbon metabolic flow of pyruvic acid flowing to the target product is dredged, and synthesis of the mitochondrial region indoor target product is finally realized. The characterization of a group of rhodotorula mitochondrial signal peptides disclosed by the invention can realize the synthesis of mitochondrial regional chamber target products, and lays a foundation for the research on the realization of metabolic engineering transformation in subcellular organelles.

The invention provides a patchouli transcription factor PcIDD2 and application of the patchouli transcription factor PcIDD2 in forward regulation of biosynthesis of patchouli ketone. The patchouli transcription factor PcIDD2 has an amino acid sequence as shown in SEQ ID NO.1; the transcription factor is a potential transcription factor with a function of promoting biosynthesis of the patchouli ketone, and has important significance in promoting efficient synthesis of the patchouli ketone.

The invention discloses a substance-based quality detection method for Xiaohuang powder, and belongs to the technical field of quality detection of traditional Chinese medicines. According to the method, standard control of a classic famous prescription is realized by constructing a multi-dimensional quality control system. The method comprises the following steps: firstly, carrying out specific identification on gardenia, divaricate saposhnikovia root, liquorice and pogostemon cablin in the prescription by adopting a thin-layer chromatography; meanwhile, a multi-index component content determination method is established, and the contents of five components including geniposide, cimicifugin, 5-O-methylvisammioside, liquiritin and glycyrrhizic acid are synchronously determined by applying a high performance liquid chromatography (HPLC). Through a system quality control strategy combining qualitative identification and quantitative analysis, not only is a quality standard system of the substance reference of the Xiaohuang powder perfected, but also reliable technical guarantee is provided for ensuring the stability and consistency of product quality. The establishment of the method establishes a standardized method system for the substance-based quality control of the classical famous prescription Xiaohuang powder.

The invention belongs to the technical field of traditional Chinese medicine research, and particularly relates to a traditional Chinese medicine composition for treating cold and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 7-11 parts of cistanche, 10-14 parts of dried ginger, 7-11 parts of pogostemon cablin, 13-17 parts of pericarpium citri reticulatae, 10-14 parts of polygonum cuspidatum, 7-11 parts of bistort rhizome, 8-12 parts of fructus forsythiae, 28-32 parts of honeysuckle, 10-14 parts of radix bupleuri, 10-14 parts of blackberry lily, 8-12 parts of notopterygium root, 7-11 parts of radix angelicae pubescentis, 7-11 parts of divaricate saposhnikovia root, 7-11 parts of schizonepeta, 7-11 parts of radix angelicae, 10-14 parts of fingered citron, 7-11 parts of perilla stem, 13-17 parts of artemisia apiacea and 10-14 parts of folium mori. The traditional Chinese medicine composition has a better curative effect on cold-dampness cold.

The invention discloses a saccharomyces cerevisiae recombinant strain based on a uracil metabolism gene URA3 as well as a construction method and application of the saccharomyces cerevisiae recombinant strain. The construction method comprises the following steps: taking saccharomyces cerevisiae rich in farnesyl pyrophosphate as an original strain, introducing a uracil metabolism gene URA3, synchronously overexpressing a ribosome biosynthetic gene UTP10 and an iron metabolism gene FIT3, and utilizing the uracil metabolism gene URA3 to express up-regulation correlation with the ribosome biosynthetic gene UTP10 and the iron metabolism gene FIT3, so as to obtain the farnesyl pyrophosphate-rich saccharomyces cerevisiae. The metabolic flux of the terpenoids is enhanced, and the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids is constructed. According to the method, a metabolic network of yeast is systematically optimized, and combined regulation and control on precursor flux distribution, protein translation capability and metal ion steady state are realized, so that the microbial synthesis efficiency of sesquiterpenoids such as patchouli alcohol and beta-elemene is remarkably improved.

The invention discloses a method for producing terpenoids, and belongs to the technical field of bioengineering. The serratia marcescens engineering bacteria for producing the semi-terpenoids or monoterpenoids are constructed, and can be used for producing isoprene, isopentenol, 1, 3, 4-triterpenes, 1, 3, 4-triterpenes and the like. The composition is prepared from 1, 8-cineole, alpha-pinene, pinene, gamma-terpinene, geraniol, (+)-limonene, (-)-limonene, myrcene, beta-ocimene, sabinene, (-)-alpha-bisabolol, (-)-alpha-bisabolol, longifolene, wallenenes, beta-elemene, farnesene, patchouli alcohol, acyclic terpene and alpha-santalene. In a 30L fermentation tank, the yield of linalool produced by the serratia engineering bacteria is 13.49 g.L <-1 >.

The invention provides a traditional Chinese medicine composition for relieving chemotherapy side effects, traditional Chinese medicine compound essential oil and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 1-9 parts of pericarpium citri reticulatae, 1-9 parts of pericarpium citri reticulatae viride, 1-9 parts of clove, 1-9 parts of fennel, 1-9 parts of ginger, 1-9 parts of pogostemon cablin and 1-9 parts of rhizoma atractylodis. The traditional Chinese medicine composition is mainly used for treating abdominal distension pain, nausea and vomiting caused by damp turbidity resistance and spleen and stomach disharmony through the effects of promoting qi circulation, removing dampness, strengthening the spleen and harmonizing the stomach. Experimental results show that the traditional Chinese medicine compound essential oil prepared from the traditional Chinese medicine composition can reduce allotriophagia behaviors of rats and improve ingestion and drinking habits, has a recovery effect on metabolite disorder caused by chemotherapy-related nausea and vomiting, and provides a theoretical basis for a supplementary treatment method of chemotherapy-related nausea and vomiting.

The invention provides a pogostemon cablin cold stress response transcription factor and application thereof in resisting cold and increasing pogostemon cablin alcohol content, and belongs to the technical field of gene engineering. The terpene metabolism cold response transcription factor PatbZIP6 and / or PatMYB5 is obtained through screening, the transcription factor obtained through screening can remarkably improve synthesis accumulation of patchouli alcohol through a genetic transformation method, the cold resistance of patchouli is improved, and the transcription factor can be used for breeding new varieties of patchouli.

The invention relates to a collateral-dredging thrombus-removing traditional Chinese medicine composition for preventing and treating stroke, vascular thrombus, arteriosclerosis, coronary heart disease or waist and leg pain. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 12-65 parts of cooked rhubarb, 10-72 parts of salvia miltiorrhiza, 12-65 parts of cortex moutan, 6-57 parts of rhizoma zingiberis, 8-50 parts of pseudo-ginseng, 15-45 parts of obscured homalomena rhizome, 10-68 parts of leech, 7-76 parts of earthworm, 15-70 parts of ligusticum wallichii, 6-52 parts of Tibetan capillary artemisia, 7-49 parts of cape jasmine, 3-25 parts of borneol, 6-75 parts of angelica sinensis, 12-65 parts of ginseng, 7-53 parts of pogostemon cablin and 10-77 parts of radix curcumae. The medicine has the effects of promoting blood circulation, removing blood stasis, dredging collaterals and removing thrombus. The traditional Chinese medicine composition can be used for preparing medicines for treating cerebral apoplexy, coronary heart disease, arteriosclerosis and static artery thrombosis diseases, or medicines for soothing channels, activating collaterals and promoting blood circulation to remove blood stasis, which are related to waist and leg pain diseases. The invention provides a prevention and treatment effective solution for people with stroke, vascular thrombosis, arteriosclerosis, coronary heart disease or waist and leg pain.

The invention relates to a method for relieving continuous cropping obstacles of ginseng and application of the method, and belongs to the technical field of agricultural planting. The method comprises the following specific steps: planting wrinkled gianthyssop herb on a field after ginseng planting, crushing the wrinkled gianthyssop herb, returning the crushed wrinkled gianthyssop herb to the field, and improving soil for subsequent ginseng planting. The method can effectively relieve continuous cropping obstacles of ginseng, increase the content of soil organic matter, nitrogen, phosphorus and potassium, adjust the pH of soil, improve the activity of soil catalase, reduce the activity of sucrase and urease, optimize the microbial community structure of soil, reduce the abundance of pathogenic bacteria and reduce the incidence rate of root rot and rust rot of ginseng; the yield and quality of ginseng are remarkably improved, the ginsenoside content is increased, meanwhile, use of chemical fertilizers and pesticides is reduced, a green and safe planting mode is achieved, and remarkable economic benefits and ecological benefits are achieved.

The present invention discloses a compound microbial agent for alleviating continuous cropping obstacles of traditional Chinese medicine crops and its application. The compound microbial agent of the present invention contains Bacillus amyloliquefaciens Y34, Serratia marcescens Y35, Bacillus amyloliquefaciens Y40 and Streptomyces SD; it can be applied to improve soil, eliminate diseases of Polygonatum odoratum, Pogostemon cablin and Cynanchum stauntonii, and promote crop growth. The compound microbial agent of the present invention can reduce soil-borne diseases during the planting process of traditional Chinese medicine, alleviate continuous cropping obstacles of traditional Chinese medicine, improve the yield and quality of traditional Chinese medicine, and has important application value especially in the cultivation and production of traditional Chinese medicine crops such as Polygonatum odoratum and Pogostemon cablin.

The invention provides a quality detection method of antiviral particles. The quality detection method adopts HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) to simultaneously determine the following components: forsythiaside A, patchouli ketone, verbascoside, mangiferin, 5-methoxy-8-hydroxypsoralen, curcumenol, luteolin-7-glucuronide, kaempferol-3-O-rutinoside, baicalin, rutin and bergamot phenol. The detection method is a quantitative determination method for 11 active components in the antiviral particles, the detection method is short in time and low in cost, the quantitative analysis method is expected to provide reference for quality control of the antiviral particles, and the clinical curative effect and safety of the antiviral particles are guaranteed.

The invention relates to the field of modernization of traditional Chinese medicines, in particular to a method for identifying a Dayuan liquid preparation. The invention respectively provides thin-layer chromatography identification methods for pogostemon cablin, bupleurum chinense and glabrous sarcandra herb in the daoyuan liquid preparation, the thin-layer chromatography identification methods for the three components are good in specificity, repeatability and stability, and effective quality control of the daoyuan liquid preparation can be realized.

The present invention provides a heat-clearing and damp-removing anti-toxic granule and a preparation method thereof, wherein the heat-clearing and damp-removing anti-toxic granule comprises a main component, which comprises, by weight, 280-320 parts of patchouli, 280-320 parts of boiled bitter almonds, 280-320 parts of mulberry leaves, 190-210 parts of cardamom, 280-320 parts of wine scutellaria, 280-320 parts of forsythia, 280-320 parts of tuckahoe, 280-320 parts of talc, 490-510 parts of coix seeds, 190-210 parts of liquorice tablets, and 90-110 parts of cicada shells. The heat-clearing and damp-removing anti-toxic granule provided by the present invention realizes heat-clearing and damp-removing anti-toxic, can inhibit lung tissue inflammation, and alleviate lung damage.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a pharmaceutical composition for treating rhinitis and a preparation method and application thereof. The invention provides a pharmaceutical composition for treating rhinitis. The pharmaceutical composition is prepared from the following components in parts by mass: 12-15 parts of astragalus membranaceus, 9-12 parts of codonopsis pilosula, 9-12 parts of houttuynia cordata, 6-9 parts of divaricate saposhnikovia root, 9-12 parts of bighead atractylodes rhizome, 6-9 parts of radix bupleuri, 6-9 parts of ginger processed pinellia, 6-9 parts of fructus forsythiae, 6-9 parts of radix angelicae, 6-9 parts of fried semen armeniacae amarae, 6-9 parts of cocklebur fruit, 6-9 parts of isatis root, 6-9 parts of pogostemon cablin, 3-6 parts of rhodiola rosea, 2-4 parts of schizonepeta, 3-6 parts of dried ginger, 3-5 parts of Chinese date and 3-6 parts of liquorice. The composition provided by the invention is effective for both allergic rhinitis and chronic sinusitis, can relieve acute symptoms and improve chronic inflammation, and overcomes the defect of insufficient pertinence of the existing traditional Chinese medicine compound.

The invention belongs to the technical field of ecological water, mainly relates to ecological water for removing fruit peculiar smell, and particularly relates to ecological water for removing fruit peculiar smell and a preparation method thereof. Neotame and glucose modified cortex phellodendri are matched with tupistra chinensis, pogostemon cablin, liquorice and clove to be decocted into a traditional Chinese medicine decoction. According to the invention, lactobacillus plantarum ATCC 8014 and bacillus subtilis ATCC 6633 are used for fermentation. The antibacterial activity of the ecological water is improved, and the bitter taste of the cortex phellodendri is effectively removed due to the synergism of the two sweetening agents, namely the neotame and the glucose. The bacillus subtilis HZ-72 antagonistic bacteria used in the invention can enhance the capability of inhibiting pathogenic bacteria. Syzygium aromaticum has antibacterial activity, pogostemon cablin has an antiviral effect, and the syzygium aromaticum and the pogostemon cablin cooperate to enhance the antibacterial The ecological water prepared by the invention can remove peculiar smell and preserve freshness, and has a great development prospect in the field of fruit preservation.

The invention relates to the technical field of food processing, in particular to a glucoraphanin-containing agastache rugosus and pericarpium citri reticulatae plant beverage formula which comprises the following raw materials: main materials: pogostemon cablin, pericarpium citri reticulatae, liquorice, mint and broccoli seed water extract; and auxiliary materials: pure natural sweet raw materials and prebiotics. According to the invention, several components in the ingredients are continuously optimized and combined, so that the pogostemon cablin and broccoli seed aqueous extract can be well fused with other composition raw materials in the ingredients, and the pogostemon cablin and dried orange peel beverage with extremely characteristic flavor and taste is formed. The flavor of the tea can be accepted by most people, and the tea has the effects of regulating qi, resolving dampness, dispelling wind, clearing heat, enhancing immunity and the like, and is especially suitable for people with low immunity to drink.

The present invention relates to the technical field of pesticides, and more particularly to a high-efficiency, low-residue pesticide composition, the active ingredients of which are formed by compounding spinetoram with patchouli ketone, wherein the mass ratio of spinetoram to patchouli ketone is 1-40:3-1. When the spinetoram and patchouli ketone are compounded in the pesticide composition of the present invention, a synergistic effect is exhibited within a certain mass ratio range, thereby improving the control effect. This synergistic effect can reduce the application dosage of chemical agents, thereby avoiding, to a certain extent, the problems of pesticide residues and environmental pollution caused by high-dose application.

The present invention discloses a combined drug for treating acne, which contains bletilla stilbene components and patchouli essential oil for simultaneous or separate administration. By combining bletilla stilbene components and patchouli essential oil in a specific ratio, the present invention significantly inhibits Propionibacterium acnes and acne inflammation, thereby playing a role in treating acne. Moreover, the effect is better than that of either bletilla stilbene components or patchouli essential oil used alone, and is more effective than adapalene gel commonly used at present, showing a synergistic effect and having practical popularization and application value.

The invention discloses a pogostemon cablin essential oil clathrate compound preparation device, and relates to the technical field of medicine preparation, the pogostemon cablin essential oil clathrate compound preparation device comprises a kettle body, the bottom of the kettle body is an arc-shaped lower sealing head structure; the flow guide cone is arranged in the center of the bottom of the kettle body, and baffles are vertically arranged on the inner wall of the kettle body in a circumferential array mode; the stirring assembly is arranged in the kettle body, and the stirring assembly is used for being matched with the flow guide cone and the baffle and is used for guiding liquid in the kettle body to circularly flow up and down; the outer circulation high-shearing assembly comprises a high-speed shearing device, a suction inlet assembly is arranged at the side bottom of the kettle body and communicated with a feeding port of the high-speed shearing device, and a backflow port is formed in the side wall of the kettle body and communicated with a discharging port of the high-speed shearing device; an outer circulation passage is formed between the kettle body and the high-speed shearing device; the ultrasonic-assisted assembly is arranged near the suction inlet assembly; and the essential oil injection pipe is arranged in the flow guide cone.

The invention discloses a traditional Chinese medicine preparation for treating chronic rhinitis and chronic nasosinusitis, and relates to the technical field of traditional Chinese medicine preparations. The felwort, the radices stellariae dichotomae, the wine-fried scutellaria baicalensis and the fried cape jasmine are used for treating middle-jiao and upper-jiao, the felwort and the radices stellariae dichotomae are used for clearing damp heat of middle-jiao and lower-jiao, and the fried cape jasmine, the wine-fried scutellaria baicalensis and the lalang grass rhizome are used for removing heat of the upper-jiao; fried cocklebur fruit, angelica dahurica and pogostemon cablin are strong in pungent power, fragrant and capable of opening orifices, and divaricate saposhnikovia root is used for ascending medicine Coix seeds and wax gourd kernels are used for removing dampness and turbidity. The traditional Chinese medicine has the effects of promoting blood circulation and removing blood stasis due to the fact that the peach kernels are independently matched, and the medicines are used together to achieve the effects of ascending lucidity, descending turbid, clearing heat and promoting diuresis.

The present invention discloses the preparation of patchouli flavonoid nanoliposomes and their application in inhalation treatment of acute lung injury, and relates to the field of medical technology. The patchouli flavonoid nanoliposomes provided by the present invention prepare patchouli flavonoids into a liposome dosage form, which has good stability and biocompatibility. The drug is administered to mice with acute lung injury, so that patchouli flavonoids can act on the mouse lungs in a targeted manner, reduce the expression of inflammatory factors such as IL-6, IL-1β and TNF-α in the mouse lungs to relieve inflammation, improve pulmonary edema, and reduce lung tissue damage, while increasing the levels of tight junction factors such as ZO-1, VE-Cadherin, E-Cadherin and Claudin5 to repair lung barrier damage. The present invention is expected to provide an effective strategy for the treatment of acute lung injury.

The invention provides a novel essential oil patch with double effects of wrinkle resistance and sleep aiding. The novel essential oil patch sequentially comprises a bottom layer, a first impermeable layer, a first essential oil layer, a second impermeable layer, a second essential oil layer, a third impermeable layer, a third essential oil layer and a sealing layer from bottom to top, the first essential oil component comprises one or a mixture of peppermint oil, clove volatile oil, angelica sinensis volatile oil, eucalyptus oil or fructus alpiniae oxyphyllae oil; the second essential oil component comprises one or a mixture of cananga odorata essential oil, lemon essential oil, pogostemon cablin essential oil, rosemary essential oil, neroli essential oil, jasmine essential oil or rose essential oil; the third essential oil component comprises one or a mixture of chamomile essential oil, lavender essential oil, bergamot essential oil or sweet orange essential oil. The essential oil patch prepared by the invention has the effects of being good in slow release effect, high in percutaneous absorption rate, safe, environment-friendly and non-irritating.

This invention relates to the field of external moxibustion devices in traditional Chinese medicine, and in particular to a moxa stick for clearing dampness using the thunder-fire method, and its preparation method. The moxa stick comprises moxa wool, compound Chinese herbal powder, and moxa leaf paper; the compound Chinese herbal powder includes principal, assistant, adjuvant, and guiding herbs; the principal herbs include Evodia rutaecarpa, Cinnamomum cassia, and Ligusticum chuanxiong; the assistant herbs include stir-fried Atractylodes lancea, Poria cocos, Polyporus umbellatus, Alisma plantago-aquatica, stir-fried Atractylodes macrocephala, Astragalus membranaceus, and Amomum villosum; the adjuvant herbs include stir-fried Phellodendron chinense, Sophora flavescens, Artemisia capillaris, Gardenia jasminoides, Akebia quinata, Pinellia ternata, Centella asiatica, and Rosa laevigata root; the guiding herbs include Angelica dahurica, Pogostemon cablin, Angelica sinensis, Paeonia lactiflora, Glycyrrhiza uralensis, and Nelumbo nucifera leaf. This invention, guided by compound formulation theory, material innovation, process optimization, and packaging design, provides a thunder-fire moxa stick with strong targeted dampness-clearing properties, stable combustion performance, safety, portability, and controllable quality, showing promising application prospects.

The present invention belongs to the field of traditional Chinese medicine technology, and specifically relates to a traditional Chinese medicine composition for preventing influenza, its preparation method, and application. The composition comprises the following raw materials in parts by weight: 6-30g of raw coix seed, 5-30g of kapok flower, 3-20g of patchouli, 3-15g of perilla leaf, 3-15g of siler, 3-15g of forsythia, 3-20g of astragalus, and 5-20g of ginger. The traditional Chinese medicine composition of the present application uses authentic Lingnan medicinal materials, is simple, convenient, inexpensive, and effective, and can treat both cold and warm effects, dispel pathogenic factors without damaging the body's health, strengthen the body's health without restraining pathogenic factors, and eliminate dampness without damaging yin. It dispels pathogenic factors and strengthens the body's health simultaneously, has a good effect on preventing influenza, can enhance the body's immunity, and has a good preventive and health-care effect.

The present invention provides a compound essential oil, an anti-corrosion system containing the same, and their uses. The compound essential oil is composed of the following components: wild chrysanthemum essential oil, jasmine essential oil, patchouli essential oil, and lemon-aldehyde-type camphor leaf essential oil. The compound essential oil has the effect of significantly inhibiting the growth of Propionibacterium acnes, Staphylococcus aureus, and Malassezia for a long time. The present invention also provides a compound anti-corrosion system, which contains the above compound essential oil, 1,2-hexanediol, and caprylyl glycol.

The invention discloses a preparation method and application of a repairing and soothing composition. Aiming at the dissolvability difference of different components in the three extracts, a step-by-step combined extraction process is adopted for extraction, that is, stephania tetrandra is subjected to composite enzymolysis and then subjected to microwave-assisted extraction, citrus peel is subjected to low-temperature fermentation and then subjected to subcritical water extraction, and pogostemon cablin leaves are subjected to supercritical CO2 extraction and then subjected to ethanol gradient elution. After the three extracts extracted by the process are compounded, the composition has excellent skin barrier repairing, moisturizing and skin relieving effects, is safe and non-irritant, and can be used for uncomfortable skin after medical beauty and season change.

The present invention discloses a patchouli alcohol synthase mutant and a method for fermenting patchouli alcohol using recombinant Escherichia coli, belonging to the field of bioengineering. The present invention modifies patchouli alcohol synthase and constructs corresponding recombinant Escherichia coli to obtain a recombinant bacterium capable of efficiently fermenting and producing patchouli alcohol, and a fermentation method thereof. The recombinant strain can ferment and produce high-value-added patchouli alcohol using inexpensive glucose as a substrate. After 96 hours of shake flask fermentation, the patchouli alcohol yield reaches 448.1 mg / L, with a yield of 39.1 mg / g DCW and a volumetric production rate of 112 mg / L / day. In a 5L fermenter, the patchouli alcohol yield can reach 2128 mg / L, with a volumetric production rate of 304 mg / L / day.

This invention provides a method for the separation, purification, and aroma quality control of biosynthesized patchouli essential oil, belonging to the field of daily chemical product purification technology. It solves the technical problems of low separation efficiency, insufficient purity, easy destruction of heat-sensitive components, and low aroma matching in existing essential oils. This method uses metabolically engineered brewer's yeast fermentation broth as raw material, sequentially undergoing pretreatment, multi-stage countercurrent extraction, three-stage high-vacuum distillation, column chromatography purification, activated carbon decolorization and deodorization, aroma component recombination, and product stabilization to obtain high-purity patchouli essential oil with high aroma matching. This invention is adapted to the three-stage distillation process of biosynthetic systems, achieving efficient separation of solvents, sesquiterpenes, and patchouli alcohol, resulting in high purity; the product aroma has a high matching degree with natural Indonesian essential oils; the process is mild throughout, can be stably scaled up from small-scale to ton-scale, and is low-cost. This invention provides a complete, efficient, and green technical solution for the industrialization of natural fragrances derived from synthetic biology.