47 results about "Calycosin" patented technology

The invention discloses a construction method and application of a specific chromatogram of a Shanhaidan pharmaceutical preparation, and belongs to the technical field of traditional Chinese medicine detection. The method comprises the following steps: preparing a test solution by using a plurality of different batches of Shanhaidan medicinal preparations in combination with high performance liquid chromatography and mass spectrometry technologies; the preparation method comprises the following steps: preparing a single reference substance solution from uridine, tanshinol, daidzin, p-hydroxybenzoic acid, puerarin, caffeic acid, 3 '-hydroxy puerarin, physcion, calycosin-7-O-beta-D-glucoside, stilbene glucoside, rosmarinic acid, alkannic acid, formononin, salvianolic acid B, daidzein and calycosin-7-one; and respectively carrying out high performance liquid chromatography analysis on the test solution and the reference solution to obtain the characteristic chromatogram of the Shanhaidan pharmaceutical preparation. The method is simple and convenient to operate, high in precision, good in reproducibility and accurate and reliable in result. And at least 16 components can be identified at one time under the same chromatographic condition, so that the detection efficiency is remarkably improved, the time and the cost are saved, and the quality of the Shanhaidan pharmaceutical preparation is comprehensively and scientifically evaluated.

The present application relates to the engineered microorganism capable of synthesizing calycosin or its derivative and its construction method and application. The present application carries out metabolic engineering modification on the Saccharomyces cerevisiae capable of synthesizing daidzein, screens the isoflavone-3'-hydroxylase capable of efficiently converting the intermediate product into calycosin, optimizes the enzyme combination, obtains the engineered Saccharomyces cerevisiae capable of efficiently synthesizing calycosin and calycosin glycoside, and realizes the calycosin glycoside yield of 47.60 mg / L after 72 h fermentation through the mode of adjusting metabolic flux and optimizing culture conditions, which lays the foundation for the industrialized production of calycosin glycoside.

The invention discloses a Jiangtangning granule fingerprint spectrum and a multi-component quantitative quality control method, and belongs to the technical field of pharmaceutical analysis. According to the method, high performance liquid chromatography is adopted, and fingerprint spectrum determination and quantitative analysis of 10 components are synchronously realized under the same chromatographic condition. The method comprises the following steps: preparing a test solution and a reference solution, carrying out gradient elution with an acetonitrile-phosphoric acid aqueous solution, and detecting under optimized chromatographic conditions. The fingerprint spectrum determines common peaks based on multiple batches of samples, and the similarity is greater than or equal to 0.9; 10 components such as calycosin-7-glucoside are measured at the same time, the separation degree is larger than or equal to 1.5, and the linear relation is good. The method solves the problems of few control indexes and low efficiency in the prior art, can comprehensively characterize the product quality, improve the detection efficiency and reduce the cost, and is suitable for quality control of the Huangtangning granules.

The invention discloses a method for establishing a vitality-preserving decoction fingerprint spectrum. The method comprises the following steps: preparing a test solution, preparing a reference solution, and obtaining the fingerprint spectrum of the vitality-preserving decoction preparation by adopting a high performance liquid chromatography. According to the chromatographic condition and system applicability test, octadecylsilane chemically bonded silica is used as a filler, acetonitrile is used as a mobile phase A, a 0.1% phosphoric acid solution is used as a mobile phase B for gradient elution, the detection wavelength is 210 nm, and the number of theoretical plates is calculated according to a calycosin-7-glucoside peak and should not be lower than 3000. The method disclosed by the invention can be used for controlling the quality of the vigor-preserving decoction preparation, the specificity and effectiveness of the quality control of the vigor-preserving decoction preparation can be remarkably improved, and the safety and reliability of clinical medication are ensured.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention belongs to the technical field of agricultural product production, and particularly relates to an astragalus membranaceus planting method based on farmyard manure. The planting method comprises the following steps: S1, soil improvement; s2, seed selection and seedling raising; s3, transplanting the seedlings; s4, field management; s5, harvesting and processing. The soil is directionally improved by applying fermented farmyard manure per mu, and the organic planting of astragalus membranaceus is realized in cooperation with standardized planting, management and processing flows. Astragalus mongholicus planted through the method is free of pesticide residues, the content of effective components such as astragaloside and calycosin-7-glucoside far exceeds the national standard, the extract content is remarkably higher than that of common astragalus mongholicus, the soil quality is continuously optimized, the yield is stable, the problems that in traditional astragalus mongholicus planting, pesticide residues exceed the standard, soil is hardened, effective components are insufficient, and the yield is low are solved, and the method is suitable for large-scale popularization and application. The method is suitable for large-scale popularization and application.

The invention discloses an NK cell culture solution and culture and application of NK cells, the NK cell culture solution comprises an activation culture solution and an amplification culture solution, the activation culture solution and the amplification culture solution respectively contain a basal culture medium and respective corresponding cell factors, and the activation culture solution also contains calycosin with the final concentration of 0.1-10 [mu] g / mL and formononetin with the final concentration of 1-20 [mu] g / mL; the amplification culture solution also contains astragaloside IV and soyasaponin I. The components such as calycosin, formononetin, astragaloside IV and soyasaponin I added into the NK cell culture fluid can maintain stable and lasting physiological activity of NK cells and slow down cell senescence, so that secretion of cell factors is promoted, the secretion level of the cell factors is higher, and the purposes of enhancing the persistence of cell activity and improving the killing rate of the NK cells are achieved.

The application discloses a composition for treating lumbar intervertebral disc herniation and repairing annulus fibrosus, relates to the technical field of traditional Chinese medicine compositions, and comprises the following raw materials: radix rehmanniae preparata, plastrum testudinis, fructus psoraleae, eucommia ulmoides oliver, walnut kernel, bee pollen, ligustilide, saikosaponin A, salvianolic acid B, ferulic acid, calycosin, stibene glycoside, prunol, loganin, hydroxysafflor yellow A and ligustilide. Experiments show that the composition can strengthen the repair of annulus fibrosus, has anti-inflammatory and analgesic effects, protects collagen, inhibits degeneration, can effectively treat lumbar intervertebral disc herniation and realize the structural repair of damaged annulus fibrosus, and provides a new technical scheme for the clinical treatment of lumbar intervertebral disc herniation.

The present invention discloses a high performance liquid chromatography fingerprint analysis method for compound Fufangteng capsule and its application, which belongs to the field of chemical analysis technology. A mixed solution of protocatechuic acid, calycosin glucoside, thorn formononetin, calycosin, and formononetin is used as a standard solution, and a methanol extract of compound Fufangteng capsule is used as a test solution for high performance liquid chromatography analysis to establish a compound Fufangteng capsule HPLC fingerprint; wherein, the high performance liquid chromatography analysis uses acetonitrile 0.2% phosphoric acid solution as the mobile phase, the elution mode is gradient elution, and the detection wavelength is 254nm. The method provided by the present invention has good repeatability and high durability, and can be used for compound Fufangteng capsule quality control.

The application discloses a kind of Shenkun Yangxue granules fingerprint and its construction method, belong to traditional Chinese medicine detection technical field.Different batches of Shenkun Yangxue granules are prepared into test sample solution;Respectively with Danshensu, ephedrine hydrochloride, syringin, protocatechuic aldehyde, coffee acid, calycosin-7-O-β-D-glucoside, loganin, comphoric acid, shisonin, delphinidin and luteolin and kaempferol control preparation single control solution;Test sample solution and control solution are analyzed by high performance liquid chromatography and high resolution mass spectrometry respectively, 18 characteristic peaks are screened out, and 12 kinds of chemical components are identified by standard comparison and fragment information analysis, to obtain Shenkun Yangxue granules fingerprint.The Shenkun Yangxue granules fingerprint established by the method can be used for the quality control detection of Shenkun Yangxue granules, which is conducive to the comprehensive monitoring of product quality, and ensures the consistency, controllability and stability of product quality.

The invention provides a method for detecting the blood concentration of Shenfukang capsules. The method comprises the following steps: respectively preparing a first detection specimen containing a serum sample and a second detection specimen containing a urine sample; the method comprises the following steps: taking a first sample to be detected and a second sample to be detected, injecting a Supersil ODS 2 chromatographic column, taking 0.1% phosphoric acid aqueous solution-methanol as a mobile phase, respectively detecting the chromatographic peak of calycosin in the first sample to be detected and the chromatographic peak of rhein in the second sample to be detected, and calculating the concentration of calycosin and the concentration of rhein in the samples according to the chromatographic peaks; the chromatographic conditions of the first detection sample are that the detection wavelength is 260 nm, and gradient elution is carried out; the chromatographic conditions of the second detection sample are as follows: the detection wavelength is 254 nm, and isocratic elution is performed. The method has excellent repeatability and high sensitivity, the operation process is simple and easy to implement, the administration scheme can be accurately adjusted and an individualized administration strategy can be implemented in close combination with clinical manifestation and various inspection results of a patient, so that the safety and effectiveness of the medication process are ensured, and the method is suitable for clinical popularization and application. And a reliable medication guarantee is provided for the treatment of patients with chronic kidney diseases.

The application discloses application of a Chinese medicine pair effective component composition of Astragalus membranaceus and Curcuma zedoary in preparation of anti-tumor metastasis drugs. The composition is composed of calycosin-7-glucoside and curcumin. The composition can effectively inhibit transendothelial invasion and vasculogenic mimicry generation of tumor cells at a non-toxic dose, and has the advantages of high efficiency and low toxicity in treating tumor metastasis. The important point of the application is that the preferred composition can significantly reduce the exosome LAP-TGF-beta1 protein loading level, thereby greatly inhibiting the tumor metastasis process caused by the exosome-mediated TGF-beta signal pathway. The finding provides a new perspective for the development of drugs for targeting exosome LAP-TGF-beta1 protein loading to resist tumor metastasis, and overcomes the defects of unclear effective components, uncontrollable proportioning and unclear treatment effect of traditional Chinese medicine compatibility. Therefore, the Chinese medicine effective component composition has application prospects in preparation of anti-tumor metastasis drugs.

The present application belongs to the field of Chinese medicine preparation, and relates to a quality detection method of a Chinese medicine compound composition for benefiting kidney and dispelling wind. The Chinese medicine compound composition for benefiting kidney and dispelling wind comprises the following components in weight fractions: 9-30 parts of raw astragalus, 6-12 parts of wine holly fruit, 3-6 parts of scorpion, 5-10 parts of fried cantharis, 9-30 parts of prince salvia, 6-12 parts of fried atractylodes, 6-30 parts of pyrrosia, 3-10 parts of safflower, 3-10 parts of chuanxiong, 15-30 parts of white flower snake tongue, 15-30 parts of poria bark, and 9-30 parts of plantago seed. The quality detection method of the Chinese medicine compound composition adopts thin layer chromatography to identify astragalus and wine holly fruit, and further improves the comprehensiveness and reliability of the quality monitoring of the Chinese medicine compound composition by determining the content of calycosin-7-glucoside, and more perfectly characterizes the effective active components and quality of the medicine, which is beneficial to the comprehensive monitoring of the active components and further guarantees the stability, consistency, controllability, safety and effectiveness of the Chinese medicine compound composition.

The application provides application of ER-alpha 46 and calycosin in preparation of ovarian cancer treatment drugs, and belongs to the field of biological medicines, and the application mines ER-alpha 46 as a new target, confirms that ER-alpha 46 is highly expressed in ovarian cancer cells, calycosin can dose-dependently up-regulate the expression of ER-alpha 46, and the two can synergistically inhibit malignant behaviors such as proliferation and clone formation of ovarian cancer cells, and have no significant toxicity to normal ovarian epithelial cells. The two can inhibit abnormal activation of the Wnt / beta-catenin pathway through a protein complex formed by ER-alpha 46, beta-catenin and BCL9, and can also regulate the expression of EMT and angiogenesis related proteins. The scheme solves the pain points of existing treatment, provides a new strategy for targeted combination therapy of ovarian cancer, and has high safety of related drugs and good clinical conversion prospect.

The invention belongs to the technical field of quality detection of traditional Chinese medicines, and particularly relates to a specific chromatogram establishment and detection method of a compound Haqing injection. The method for establishing the characteristic spectrum comprises the following steps: a, preparing a reference substance solution, namely precisely weighing an amygdalin reference substance, a calycosin-7-glucoside reference substance and a praeruptorin A reference substance, and adding methanol for dissolving to obtain the reference substance solution; b, preparation of a test solution: precisely measuring the compound Haqing injection, and preparing the test solution by adopting a solid-phase extraction instrument; and c, measuring: respectively measuring the test solution and the reference solution, injecting into a liquid chromatograph, and recording a chromatogram to obtain a characteristic spectrum formed by 10 common characteristic peaks. The method is stable, high in precision and good in reproducibility, a rapid and comprehensive detection means is provided for quality control of the compound Haqing injection, and a scientific experimental basis is provided for research of a quality evaluation system of the compound Haqing injection.

The application belongs to the technical field of medicine quality control, and relates to a construction method and quality detection method of high-performance liquid chromatography characteristic spectrum of a traditional Chinese medicine preparation for treating chronic liver disease; comprising the following steps: step S1, preparation of reference solution: morroniside control product is weighed, precisely weighed and determined, and 50% methanol is used to prepare the reference solution; preparation of test solution: the prepared traditional Chinese medicine preparation is finely ground, mixed with 50% methanol, ultrasonically treated, and then diluted, filtered and obtained; step S2, high-performance liquid chromatography detection is performed on the reference solution and the test solution respectively, and the characteristic spectrum of the traditional Chinese medicine preparation for treating chronic liver disease is obtained; the detection wavelength is 240 nm; the characteristic spectrum comprises eight characteristic peaks, wherein peak 1 is 5-hydroxymethyl furfural, peak 2 (S) is morroniside, peak 3 is loganin, peak 5 is spinooside, peak 6 is calycosin-7-glucoside, peak 7 is styrine, and peak 8 is salvianolic acid B.

The application discloses a method for simultaneously controlling the qualitative and quantitative overall quality of a Huangqi Guizhi Wuwu Decoction (HGWD) reference sample, and the method is characterized in that: a water decoction process optimized through orthogonal tests is used to prepare HGWD decoctions and freeze-dried powder reference samples of multiple batches of medicinal material combinations from different producing areas; an HPLC characteristic chromatogram is established, 15 common peaks are calibrated by taking cinnamyl aldehyde as a reference peak; based on the same reference sample, the contents of eight index components, i.e., gallic acid, peony lactone glycoside, paeoniflorin, calycosin-7-glucoside, cinnamic acid, cinnamyl aldehyde, formononetin and 6-gingerol, are simultaneously determined by using a multi-wavelength HPLC, and the content of astragaloside A is determined by using an HPLC-ELSD method; the qualitative and quantitative overall quality of the reference sample is simultaneously controlled through characteristic chromatogram similarity evaluation and multi-index component content consistency evaluation. The application realizes the whole-process value transmission evaluation from medicinal material raw materials to final products, and provides a scientific basis for formulating the quality standard of the HGWD reference sample and developing granules.

The application provides a preparation method of a test sample in a content determination method of Astragalus membranaceus, which comprises the following steps: a, taking Astragalus membranaceus medicinal material powder, and sieving; b, placing the powder into a stoppered conical flask, adding 20-100 ml of 1-6 % ammonia solution-methanol solution (4 ml of ammonia solution is taken, 50 % methanol is added to 100 ml, and shaking is performed), weighing, ultrasonic extraction (250 W, 40 kHz) is performed for 10-90 min, cooling, weighing again, supplementing the lost weight with 1-6 % ammonia solution-methanol solution, shaking, filtering, and taking the filtrate, so that the test sample is obtained. The application further provides a content determination method of calycosin-7-glucoside in Astragalus membranaceus. The optimized method is stable and feasible, simple in operation, high in extraction efficiency, short in analysis time, low in detection cost, and accurate in determination, and can provide a scientific basis for improving the content determination method of calycosin-7-glucoside in Astragalus membranaceus in Chinese Pharmacopoeia.

The present application relates to the technical field of traditional Chinese medicine quality detection, and specifically discloses a detection method for Angelica drink composition, which comprises the following steps: (1) preparing a test sample solution; (2) preparing a mixed reference sample solution and / or a paeonol reference sample solution; (3) determining the test sample solution and the paeonol reference sample solution and / or the mixed reference sample solution to obtain the content of paeonol; (4) taking paeonol as an internal standard substance, and calculating the contents of caffeic acid, ferulic acid, glycyrrhizin, calycosin-7-glucoside, epimedoside, senkyunol I, rosmarinic acid, 5-O-methylvisamminol glycoside and glycyrrhizic acid by using an internal standard method; the chromatographic column of a liquid chromatograph is made of octadecylsilane bonded silica gel as a stationary phase, methanol as a mobile phase A, and a 0.06-0.2 vol% phosphoric acid aqueous solution as a mobile phase B for gradient elution. The detection method is practical, simple to operate, fast in testing, accurate and low in cost.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a rapid detection method for the content of calycosin-7-glucoside in astragalus formula granules, and the rapid detection method specifically comprises the following steps: detecting the content of calycosin-7-glucoside in a test sample by using a high performance liquid chromatography; detecting the content of total flavonoids in the test sample by using an ultraviolet spectrophotometric method; constructing a linear regression equation between the calycosin-7-glucoside content and the total flavone content by taking the total flavone content as a horizontal coordinate and the total flavone content / calycosin-7-glucoside content as a vertical coordinate; detecting the content of total flavonoids in a sample to be detected by using ultraviolet spectrophotometry, and substituting the content of the total flavonoids into the linear regression equation to calculate a speculated value of the content of calycosin-7-glucoside, namely the content of the calycosin-7-glucoside in the sample to be detected. According to the method, the regression equation between the ultraviolet and liquid phase results is constructed, the subsequent detection time of calycosin-7-glucoside in the astragalus membranaceus formula granules is shortened, the method is stable, the precision is high, and rapid and reliable quality control can be achieved.

The invention discloses an HPLC specific chromatogram and component content detection method of a radix astragali-lepidii heart-strengthening capsule, and belongs to the technical field of quality control of traditional Chinese medicines. The establishment method of the HPLC characteristic chromatogram of the radix astragali-lepidium meyenii heart-strengthening capsule comprises the following steps: preparing a test solution from the content of the radix astragali-lepidium meyenii heart-strengthening capsule; the preparation method comprises the following steps: respectively weighing adenosine, guanosine, tanshinol, protocatechuic acid, protocatechuic aldehyde, catechin, 4-methoxysalicylaldehyde, calycosin glucoside, narirutin, hesperidin, rosmarinic acid, alkannic acid, salvianolic acid B, apigenin and formononetin, and preparing a mixed reference solution; and carrying out HPLC detection analysis on the test solution and the mixed reference substance solution to obtain the HPLC characteristic chromatogram of the astragalus membranaceus-lepidium monnieri heart-strengthening capsule. The method has the advantages of strong specificity, high precision, good stability and good repeatability, can realize characteristic spectrum identification and multi-component content control of the astragalus membranaceus-lepidium monnieri heart-strengthening capsule at the same time, and provides a reliable basis for production quality supervision and clinical medication safety of the astragalus membranaceus-lepidium monnieri heart-strengthening capsule.

The application discloses application of callilepis, cordyceps polysaccharide or calyx formosanum isoflavone or combination of callilepis, cordyceps polysaccharide and calyx formosanum isoflavone in preparation of a medicine for relieving vomitoxin toxicity. According to the application, the callilepis, cordyceps polysaccharide and calyx formosanum isoflavone are used alone and in combination as a feed additive, a medicine for relieving vomitoxin toxicity or a detoxicant, repair vomitoxin-induced cell inflammation and damage of liver, intestinal tract and kidney through single and combined action, can effectively relieve vomitoxin toxicity on animals in a safe dosage, and thus improve the health condition of animals.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a microbial preparation and application of the microbial preparation in preparation of an astragalus mongholicus fermented product with the effects of reducing blood sugar and blood fat and high bioaccessibility. The microbial preparation is prepared from lactobacillus mucus fermentation FJ701 and leuconostoc mesenteroides XD054. The preparation method comprises the following steps: performing enzymolysis on astragalus membranaceus, inoculating a microbial preparation and fermenting to obtain an astragalus membranaceus fermented product; the Astragalus mongholicus fermented product has high content of total polyphenol, total saponin and polysaccharide, and can inhibit pancreatic lipase activity and alpha-glucosidase activity, and in vitro digestion tests find that the scavenging rates of formononin, total polyphenol, total saponin and superoxide anion free radicals are high in bioaccessibility; rat experiments find that the concentration of calycosin-7-glucoside in blood in the astragalus membranaceus fermentation product is high, various active ingredients such as syringin 3-robiniobioside in serum are increased, and the anti-inflammatory and antioxidant capacities are improved.

The application discloses a medicine for treating pancreatitis and a preparation method thereof, and the medicine contains astragaloside and calycosin-7-glucoside as active ingredients, and the weight ratio of the astragaloside and the calycosin-7-glucoside is 1-2:4-7. The astragaloside and the calycosin-7-glucoside are extracted from traditional Chinese medicine components through a large number of tests, and the combination of the two in a certain proportion has a good effect on treating acute pancreatitis, has no toxic side effect and is low in cost.

The application discloses a quality detection method of a Yishen Qingli Huoxue recipe, which comprises preparation of a test sample solution, preparation of a standard solution, preparation of a standard curve and content determination. The application screens an optimal test sample preparation method, a preparation method of a control sample and optimal chromatographic conditions and mass spectrometric analysis conditions through a large number of experiments, and establishes a content determination method of the Yishen Qingli Huoxue recipe, which can simultaneously determine the contents of 11 compounds with different structural types, such as resveratrol, rhein, formononetin, aloe-emodin, emodin, chlorogenic acid, polydatin, astilbin, hyperoside, calycosin-7-glucoside and beta-ecdysterone. The method is favorable for comprehensively monitoring the quality of the Yishen Qingli Huoxue recipe and guaranteeing clinical curative effects. Moreover, the method has the advantages of good stability, high accuracy, good repeatability and high precision.

The invention discloses a traditional Chinese medicine monomer composition for treating atherosclerosis derived from Yang tonifying and Huanwu decoction, a traditional Chinese medicine preparation prepared from the traditional Chinese medicine monomer composition and application of the traditional Chinese medicine monomer composition, and the traditional Chinese medicine monomer composition is composed of three monomers, namely, poplatin, daidzein and formononetin, and the concentration ratio of all the monomers is (0.8-1.2): (2.5-3.5): (0.8-1.2). According to the application, modern pharmacy and traditional Chinese medicine theories are creatively fused, a'classical prescription-blood entering component-action target-efficacy verification 'research system is established, and the core components (Yang-tonifying and Yang-restoring decoction) for treating atherosclerosis are successfully screened out from the Yang-tonifying and Yang-restoring decoction (Yang-tonifying and Yang-restoring decoction, daidzein and formononetin). The blood fat metabolism can be obviously improved, plaque deposition is reduced, and inflammatory response is inhibited. By means of serum pharmaceutical chemistry and network pharmacology methods, modern transformation of a traditional compound is achieved, and a new multi-target intervention strategy is provided for atherosclerosis treatment.

The invention discloses application of calycosin-7-glucoside in preparation of a medicine for inhibiting Treg cell infiltration in liver cancer, relates to the field of biological medicine, and discloses application of calycosin-7-glucoside in preparation of a medicine for inhibiting regulatory T cell (Treg) infiltration in hepatocellular carcinoma. The calycosin-7-glucoside realizes the application by inhibiting the activity and / or expression of matrix metalloproteinase 9 (MMP9); according to the application of calycosin-7-glucoside in preparation of the medicine for inhibiting Treg cell infiltration in liver cancer, the new functions of targeting tumor immune microenvironment and specifically inhibiting Treg cell infiltration of the traditional Chinese medicine active ingredient CG are found and confirmed for the first time, the medicinal value of CG is expanded, and a brand new direction is provided for clinical transformation of CG.

The invention discloses a calycosin-7-glucoside PLGA hydrogel applied to glaucoma surgery, and relates to the technical field of biological medicine, and the calycosin-7-glucoside PLGA hydrogel is prepared from the following components by weight: 1-5 parts of calycosin-7-glucoside, 20-40 parts of PLGA, 0.1-3 parts of an emulsion stabilizer, 3-8 parts of a hydrogel matrix, and 3-8 parts of a cross-linking agent. Through the construction of a dual slow-release system, the sustained release time is long, and the peak period of scar hyperplasia after operation can be covered; the semi-solid gel is attached to the sclera flap in a form, so that the adhesiveness of the surgical site is improved, and the side effects of the whole body and the secondary surgical rate can be reduced; the biocompatibility is excellent, no obvious toxicity or stimulation exists, and complications (such as low intraocular pressure and inflammation) of traditional anti-metabolic drugs can be avoided.

The application discloses a kind of HPLC fingerprint detection methods of traditional Chinese medicine, the traditional Chinese medicine is made of ten traditional Chinese medicinal materials such as radix codonopsis, atractylodes, poria, licorice, angelica, chuanxiong, white peony root, prepared rehmannia, astragalus, cassia, with the effect of tonifying qi and blood, the fingerprint established in the application includes 11 common characteristic peaks, respectively belong to ten medicinal materials, so it can identify all ten medicinal materials in traditional Chinese medicine, and also identifies 5 components therein, which are glycyrrhizin, ammonium glycyrrhizinate, verbascoside, calycosin-7-glucoside and astragaloside A respectively, so the application can more comprehensively control drug quality, solve the phenomenon of fake and inferior drugs, and ensure the safety and effectiveness of clinical use.

The invention discloses a composition which is used for preventing and / or treating cholestasis hepatic fibrosis and contains astragaloside I and calycosin-7-glucoside. The mass concentration ratio of the astragaloside I to the calycosin-7-glucoside is (1-5): (40-60), or (20-40): (0.5-2), or (40-60): (0.5-15). The composition disclosed by the invention has an excellent effect of resisting cholestasis hepatic fibrosis, and can be used for improving serum liver functions and bile duct reactions and reducing collagen deposition and inflammatory reactions.