26 results about "Calycosin" patented technology

The present application relates to the engineered microorganism capable of synthesizing calycosin or its derivative and its construction method and application. The present application carries out metabolic engineering modification on the Saccharomyces cerevisiae capable of synthesizing daidzein, screens the isoflavone-3'-hydroxylase capable of efficiently converting the intermediate product into calycosin, optimizes the enzyme combination, obtains the engineered Saccharomyces cerevisiae capable of efficiently synthesizing calycosin and calycosin glycoside, and realizes the calycosin glycoside yield of 47.60 mg / L after 72 h fermentation through the mode of adjusting metabolic flux and optimizing culture conditions, which lays the foundation for the industrialized production of calycosin glycoside.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

The invention belongs to the technical field of agricultural product production, and particularly relates to an astragalus membranaceus planting method based on farmyard manure. The planting method comprises the following steps: S1, soil improvement; s2, seed selection and seedling raising; s3, transplanting the seedlings; s4, field management; s5, harvesting and processing. The soil is directionally improved by applying fermented farmyard manure per mu, and the organic planting of astragalus membranaceus is realized in cooperation with standardized planting, management and processing flows. Astragalus mongholicus planted through the method is free of pesticide residues, the content of effective components such as astragaloside and calycosin-7-glucoside far exceeds the national standard, the extract content is remarkably higher than that of common astragalus mongholicus, the soil quality is continuously optimized, the yield is stable, the problems that in traditional astragalus mongholicus planting, pesticide residues exceed the standard, soil is hardened, effective components are insufficient, and the yield is low are solved, and the method is suitable for large-scale popularization and application. The method is suitable for large-scale popularization and application.

The application discloses a composition for treating lumbar intervertebral disc herniation and repairing annulus fibrosus, relates to the technical field of traditional Chinese medicine compositions, and comprises the following raw materials: radix rehmanniae preparata, plastrum testudinis, fructus psoraleae, eucommia ulmoides oliver, walnut kernel, bee pollen, ligustilide, saikosaponin A, salvianolic acid B, ferulic acid, calycosin, stibene glycoside, prunol, loganin, hydroxysafflor yellow A and ligustilide. Experiments show that the composition can strengthen the repair of annulus fibrosus, has anti-inflammatory and analgesic effects, protects collagen, inhibits degeneration, can effectively treat lumbar intervertebral disc herniation and realize the structural repair of damaged annulus fibrosus, and provides a new technical scheme for the clinical treatment of lumbar intervertebral disc herniation.

The application discloses application of a Chinese medicine pair effective component composition of Astragalus membranaceus and Curcuma zedoary in preparation of anti-tumor metastasis drugs. The composition is composed of calycosin-7-glucoside and curcumin. The composition can effectively inhibit transendothelial invasion and vasculogenic mimicry generation of tumor cells at a non-toxic dose, and has the advantages of high efficiency and low toxicity in treating tumor metastasis. The important point of the application is that the preferred composition can significantly reduce the exosome LAP-TGF-beta1 protein loading level, thereby greatly inhibiting the tumor metastasis process caused by the exosome-mediated TGF-beta signal pathway. The finding provides a new perspective for the development of drugs for targeting exosome LAP-TGF-beta1 protein loading to resist tumor metastasis, and overcomes the defects of unclear effective components, uncontrollable proportioning and unclear treatment effect of traditional Chinese medicine compatibility. Therefore, the Chinese medicine effective component composition has application prospects in preparation of anti-tumor metastasis drugs.

The application provides application of ER-alpha 46 and calycosin in preparation of ovarian cancer treatment drugs, and belongs to the field of biological medicines, and the application mines ER-alpha 46 as a new target, confirms that ER-alpha 46 is highly expressed in ovarian cancer cells, calycosin can dose-dependently up-regulate the expression of ER-alpha 46, and the two can synergistically inhibit malignant behaviors such as proliferation and clone formation of ovarian cancer cells, and have no significant toxicity to normal ovarian epithelial cells. The two can inhibit abnormal activation of the Wnt / beta-catenin pathway through a protein complex formed by ER-alpha 46, beta-catenin and BCL9, and can also regulate the expression of EMT and angiogenesis related proteins. The scheme solves the pain points of existing treatment, provides a new strategy for targeted combination therapy of ovarian cancer, and has high safety of related drugs and good clinical conversion prospect.

The application discloses a method for simultaneously controlling the qualitative and quantitative overall quality of a Huangqi Guizhi Wuwu Decoction (HGWD) reference sample, and the method is characterized in that: a water decoction process optimized through orthogonal tests is used to prepare HGWD decoctions and freeze-dried powder reference samples of multiple batches of medicinal material combinations from different producing areas; an HPLC characteristic chromatogram is established, 15 common peaks are calibrated by taking cinnamyl aldehyde as a reference peak; based on the same reference sample, the contents of eight index components, i.e., gallic acid, peony lactone glycoside, paeoniflorin, calycosin-7-glucoside, cinnamic acid, cinnamyl aldehyde, formononetin and 6-gingerol, are simultaneously determined by using a multi-wavelength HPLC, and the content of astragaloside A is determined by using an HPLC-ELSD method; the qualitative and quantitative overall quality of the reference sample is simultaneously controlled through characteristic chromatogram similarity evaluation and multi-index component content consistency evaluation. The application realizes the whole-process value transmission evaluation from medicinal material raw materials to final products, and provides a scientific basis for formulating the quality standard of the HGWD reference sample and developing granules.

The application provides a preparation method of a test sample in a content determination method of Astragalus membranaceus, which comprises the following steps: a, taking Astragalus membranaceus medicinal material powder, and sieving; b, placing the powder into a stoppered conical flask, adding 20-100 ml of 1-6 % ammonia solution-methanol solution (4 ml of ammonia solution is taken, 50 % methanol is added to 100 ml, and shaking is performed), weighing, ultrasonic extraction (250 W, 40 kHz) is performed for 10-90 min, cooling, weighing again, supplementing the lost weight with 1-6 % ammonia solution-methanol solution, shaking, filtering, and taking the filtrate, so that the test sample is obtained. The application further provides a content determination method of calycosin-7-glucoside in Astragalus membranaceus. The optimized method is stable and feasible, simple in operation, high in extraction efficiency, short in analysis time, low in detection cost, and accurate in determination, and can provide a scientific basis for improving the content determination method of calycosin-7-glucoside in Astragalus membranaceus in Chinese Pharmacopoeia.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a rapid detection method for the content of calycosin-7-glucoside in astragalus formula granules, and the rapid detection method specifically comprises the following steps: detecting the content of calycosin-7-glucoside in a test sample by using a high performance liquid chromatography; detecting the content of total flavonoids in the test sample by using an ultraviolet spectrophotometric method; constructing a linear regression equation between the calycosin-7-glucoside content and the total flavone content by taking the total flavone content as a horizontal coordinate and the total flavone content / calycosin-7-glucoside content as a vertical coordinate; detecting the content of total flavonoids in a sample to be detected by using ultraviolet spectrophotometry, and substituting the content of the total flavonoids into the linear regression equation to calculate a speculated value of the content of calycosin-7-glucoside, namely the content of the calycosin-7-glucoside in the sample to be detected. According to the method, the regression equation between the ultraviolet and liquid phase results is constructed, the subsequent detection time of calycosin-7-glucoside in the astragalus membranaceus formula granules is shortened, the method is stable, the precision is high, and rapid and reliable quality control can be achieved.

The application discloses application of callilepis, cordyceps polysaccharide or calyx formosanum isoflavone or combination of callilepis, cordyceps polysaccharide and calyx formosanum isoflavone in preparation of a medicine for relieving vomitoxin toxicity. According to the application, the callilepis, cordyceps polysaccharide and calyx formosanum isoflavone are used alone and in combination as a feed additive, a medicine for relieving vomitoxin toxicity or a detoxicant, repair vomitoxin-induced cell inflammation and damage of liver, intestinal tract and kidney through single and combined action, can effectively relieve vomitoxin toxicity on animals in a safe dosage, and thus improve the health condition of animals.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a microbial preparation and application of the microbial preparation in preparation of an astragalus mongholicus fermented product with the effects of reducing blood sugar and blood fat and high bioaccessibility. The microbial preparation is prepared from lactobacillus mucus fermentation FJ701 and leuconostoc mesenteroides XD054. The preparation method comprises the following steps: performing enzymolysis on astragalus membranaceus, inoculating a microbial preparation and fermenting to obtain an astragalus membranaceus fermented product; the Astragalus mongholicus fermented product has high content of total polyphenol, total saponin and polysaccharide, and can inhibit pancreatic lipase activity and alpha-glucosidase activity, and in vitro digestion tests find that the scavenging rates of formononin, total polyphenol, total saponin and superoxide anion free radicals are high in bioaccessibility; rat experiments find that the concentration of calycosin-7-glucoside in blood in the astragalus membranaceus fermentation product is high, various active ingredients such as syringin 3-robiniobioside in serum are increased, and the anti-inflammatory and antioxidant capacities are improved.

The application discloses a medicine for treating pancreatitis and a preparation method thereof, and the medicine contains astragaloside and calycosin-7-glucoside as active ingredients, and the weight ratio of the astragaloside and the calycosin-7-glucoside is 1-2:4-7. The astragaloside and the calycosin-7-glucoside are extracted from traditional Chinese medicine components through a large number of tests, and the combination of the two in a certain proportion has a good effect on treating acute pancreatitis, has no toxic side effect and is low in cost.

The application discloses a quality detection method of a Yishen Qingli Huoxue recipe, which comprises preparation of a test sample solution, preparation of a standard solution, preparation of a standard curve and content determination. The application screens an optimal test sample preparation method, a preparation method of a control sample and optimal chromatographic conditions and mass spectrometric analysis conditions through a large number of experiments, and establishes a content determination method of the Yishen Qingli Huoxue recipe, which can simultaneously determine the contents of 11 compounds with different structural types, such as resveratrol, rhein, formononetin, aloe-emodin, emodin, chlorogenic acid, polydatin, astilbin, hyperoside, calycosin-7-glucoside and beta-ecdysterone. The method is favorable for comprehensively monitoring the quality of the Yishen Qingli Huoxue recipe and guaranteeing clinical curative effects. Moreover, the method has the advantages of good stability, high accuracy, good repeatability and high precision.

The invention discloses a traditional Chinese medicine monomer composition for treating atherosclerosis derived from Yang tonifying and Huanwu decoction, a traditional Chinese medicine preparation prepared from the traditional Chinese medicine monomer composition and application of the traditional Chinese medicine monomer composition, and the traditional Chinese medicine monomer composition is composed of three monomers, namely, poplatin, daidzein and formononetin, and the concentration ratio of all the monomers is (0.8-1.2): (2.5-3.5): (0.8-1.2). According to the application, modern pharmacy and traditional Chinese medicine theories are creatively fused, a'classical prescription-blood entering component-action target-efficacy verification 'research system is established, and the core components (Yang-tonifying and Yang-restoring decoction) for treating atherosclerosis are successfully screened out from the Yang-tonifying and Yang-restoring decoction (Yang-tonifying and Yang-restoring decoction, daidzein and formononetin). The blood fat metabolism can be obviously improved, plaque deposition is reduced, and inflammatory response is inhibited. By means of serum pharmaceutical chemistry and network pharmacology methods, modern transformation of a traditional compound is achieved, and a new multi-target intervention strategy is provided for atherosclerosis treatment.

The invention discloses application of calycosin-7-glucoside in preparation of a medicine for inhibiting Treg cell infiltration in liver cancer, relates to the field of biological medicine, and discloses application of calycosin-7-glucoside in preparation of a medicine for inhibiting regulatory T cell (Treg) infiltration in hepatocellular carcinoma. The calycosin-7-glucoside realizes the application by inhibiting the activity and / or expression of matrix metalloproteinase 9 (MMP9); according to the application of calycosin-7-glucoside in preparation of the medicine for inhibiting Treg cell infiltration in liver cancer, the new functions of targeting tumor immune microenvironment and specifically inhibiting Treg cell infiltration of the traditional Chinese medicine active ingredient CG are found and confirmed for the first time, the medicinal value of CG is expanded, and a brand new direction is provided for clinical transformation of CG.

The application discloses a kind of HPLC fingerprint detection methods of traditional Chinese medicine, the traditional Chinese medicine is made of ten traditional Chinese medicinal materials such as radix codonopsis, atractylodes, poria, licorice, angelica, chuanxiong, white peony root, prepared rehmannia, astragalus, cassia, with the effect of tonifying qi and blood, the fingerprint established in the application includes 11 common characteristic peaks, respectively belong to ten medicinal materials, so it can identify all ten medicinal materials in traditional Chinese medicine, and also identifies 5 components therein, which are glycyrrhizin, ammonium glycyrrhizinate, verbascoside, calycosin-7-glucoside and astragaloside A respectively, so the application can more comprehensively control drug quality, solve the phenomenon of fake and inferior drugs, and ensure the safety and effectiveness of clinical use.

The invention relates to a method for determining the content of effective components in a Kunxinning preparation. Comprising the following steps: step 1, preparing a reference substance solution, namely respectively taking a proper amount of one or two or more of catalpol, rehmannia glutinosa liboside D, paeoniflorin, calycosin-7-glucoside, (-)-syringinol-4-O-beta-D-furanselinosyl-(1-2)-beta-D-glucopyranoside, curculigoside, icariin and baohuoside I reference substances; preparing a reference substance solution; step 2, preparation of a test solution: taking a Kunxinning preparation, weighing the Kunxinning preparation, placing the Kunxinning preparation in a measuring flask, adding methanol to dilute the Kunxinning preparation to a scale, filtering the Kunxinning preparation, and taking a subsequent filtrate; step 3, a determination method: respectively and precisely sucking the reference substance solution and the test solution, injecting the solutions into a high performance liquid chromatograph, and determining component types and / or component contents; the chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a filler, water is used as a mobile phase A, acetonitrile is used as a mobile phase B, gradient elution is performed, the flow velocity is 0.5-1.5 mL / min, the column temperature is 25-35 DEG C, the detection wavelength is 200-300 nm, and the sample size is 10-30 [mu] L.

The invention discloses a construction method and application of an HPLC (High Performance Liquid Chromatography) specific chromatogram of a leech snake collateral dredging capsule, and belongs to the technical field of quality analysis of traditional Chinese medicine compound preparations. According to the method, nine reference substances including tanshinol, 3 '-hydroxy puerarin, puerarin, 3'-methoxy puerarin, daidzin, calycosin-7-O-beta-D-glucoside, rosmarinic acid, formononin and alkannic acid are selected, a high performance liquid chromatography-mass spectrometry technology is adopted, and the leech snake collateral dredging capsules in different batches are subjected to systematic analysis. The specific chromatogram of the preparation is established. The method established by the invention is high in precision, good in reproducibility and accurate and reliable in result; the method has the advantages that nine characteristic components can be simultaneously identified under a single chromatographic condition, the analysis efficiency is greatly improved, multiple components in a preparation can be synchronously monitored, the method can be incorporated into enterprise quality standards, and the uniformity and the stability of product quality are ensured. The implementation of the invention provides a scientific basis for improving the quality standard of the leech snake collateral dredging capsule, and has an important value for guaranteeing the clinical curative effect and medication safety of the preparation.

The invention provides an application of calycosin-7-glucoside in preparation of a medicine for relieving ulcerative colitis. The invention finds that the calycosin-7-glucoside can relieve ulcerative colitis by adjusting the activity of COX-II enzyme. On one hand, the calycosin glucoside promotes the homeostasis of intestinal epithelial cells by increasing the expression quantity of a downstream product, namely 12 (S)-HHT, of COX-II enzyme in vivo; on the other hand, generation of TxA2 can be promoted, the blood coagulation process is accelerated, and then the pathological state of ulcerative colitis is improved. Animal experiments further find that the calycosin-7-glucoside can significantly inhibit the UC inflammatory reaction and improve related disease symptoms at a low oral dosage. The calycosin-7-glucoside has the advantages of small toxic and side effects, low dosage and the like, and a new treatment scheme is provided for treatment of ulcerative colitis.

The invention discloses radix astragali and radix ginseng palpitation relieving granules and a quality detection method thereof. The optimal forming process of the radix astragali and radix ginseng palpitation relieving granules is screened out through a large number of experiments. According to the invention, the optimal thin-layer chromatography development condition is screened out, and qualitative identification research is carried out on salvia miltiorrhiza, radix astragali preparata and ligusticum wallichii in the quality detection method of the radix astragali, radix astragali and radix astragali palpitation relieving granules. According to the present invention, the optimal fluidity composition, the gradient elution mode and other chromatographic conditions are screened through a large number of experiments, the fingerprint detection method with characteristics of high precision, good stability and high accuracy is established, and the contents of the effective components such as calycosin-7-glucoside, ferulic acid, dihydroquercetin, ammonium glycyrrhizinate, nardosinone and the like can be simultaneously determined; and a quality detection method is provided for ensuring the safety and the effectiveness.

This invention discloses a verbascoside isoflavone composition and its application in anti-hepatocellular carcinoma and immune remodeling, relating to the field of natural drug formulation technology. The active ingredient of this composition consists of verbascoside, rhodioloside, and taraxasterol in a mass ratio of 42:37:11. The pharmaceutical excipients of the composition consist of pharmaceutical trehalose, sulfobutyl-β-cyclodextrin, and hydroxypropyl-β-cyclodextrin in a mass ratio of 0.4:0.5:0.9. The mass ratio of the pharmaceutical excipients to the total active ingredient is 1.8:1. This invention constructs a multi-target active system through the precise compounding of three plant monomers, synergistically inhibiting hepatocellular carcinoma and regulating immunity. Low-temperature purification using a composite enzymatic hydrolysis coupled with macroporous resin ensures strict purity control and improves bioavailability. A multi-cyclodextrin complex with trehalose is used to construct an inclusion carrier, which improves water solubility and stability through stepwise encapsulation. Gradient freeze-drying is then used to form a powder for injection, which is rapidly and clearly reconstituted. The simplified excipient ratio reduces the metabolic burden on the liver and is suitable for the injection administration needs of hepatocellular carcinoma patients.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a preparation method of an astragalus mongholicus fermentation product and application of the obtained astragalus mongholicus fermentation product in preparation of products for reducing blood sugar and blood fat and promoting absorption. The preparation method specifically comprises the following steps: (1) uniformly mixing radix astragali powder with water, and sterilizing to obtain a radix astragali aqueous solution; and (2) inoculating strains into the radix astragali aqueous solution, and carrying out ultrasonic-assisted fermentation, so as to obtain the radix astragali fermented product. The obtained astragalus mongholicus fermentation product has strong active hydroxyl free radical scavenging rate, iron ion reducing capacity and ABTS < + > free radical scavenging rate; the activity of inhibiting alpha-glucosidase, DPP-IV (dipeptidyl peptidase-IV) and pancreatic lipase is better; the content release of calycosin, total polyphenols and total flavonoids in the intestinal digestion process can be obviously improved.

The invention belongs to the technical field of genetic engineering and enzymology, and particularly relates to isopentenyl transferase and application thereof to preparation of isopentenyl isoflavone. The isopentenyl transferase FoPT2 with the amino acid sequence shown as SEQ ID NO.2 is verified for the first time to show catalytic activity on 6 isoflavone compounds (genistein, dihydrogenistein, biochanin A, calycosin, genistin and 3 ', 4', 7-trihydroxy isoflavone), 21 isopentenyl products are generated in total, the structures of 10 products are analyzed, and 3 products are synthesized for the first time. The isopentenylation modification comprises 13 kinds of mono-isopentenylation (C- / O-connection) and 8 kinds of diisopentenylation (C-O connection / C-C connection), modification sites comprise C-6, 7-O, C-8, 4 '-O and the like, and wide regioselectivity is shown. By optimizing reaction conditions, the catalytic efficiency of FoPT2 is improved, the preparation of isopentenyl isoflavone with various structures is facilitated, and a new possibility is provided for isopentenyl isoflavone products.

The invention provides a method for determining the content of multiple components in an Aidi preparation, and belongs to the technical field of medicines. The Aidi preparation is prepared from traditional Chinese medicinal materials including ginseng, cantharides, astragalus membranaceus and acanthopanax. The content determination method comprises the following steps: simultaneously determining cantharidin, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rd, isofraxidin, syringin, formononetin, astragaloside, chlorogenic acid, eleutheroside E, calycosin-7-glucoside, astragaloside II, neochlorogenic acid, cryptochlorogenic acid and eleutheroside E1 in the Aidi preparation by adopting liquid chromatography-mass spectrometry; the composition is prepared from effective components such as ginsenoside F3, ginsenoside Rg2, azelaic acid and the like. Compared with the prior art, the method has the advantages that the contents of the 20 effective components in the Aidi preparation are simultaneously measured under the same chromatographic condition through liquid chromatography-mass spectrometry, the method is convenient and rapid, the requirement for product quality detection is greatly improved, the method is more suitable for quality control of drugs in the future, and the drug quality and the clinical drug effect are guaranteed.

The invention relates to the technical field of nano-drugs, and discloses a lock-release switching type triptolide nano-micelle as well as a preparation method and application thereof. The preparation method of the triptolide nano-micelle comprises the following steps: S1, carrying out esterification modification on a polyamide-amine dendritic polymer by using folic acid to obtain FA-PAMAM; s2: S2-1, dissolving the FA-PAMAM, the triptolide and the calycosin into a solvent, and rotating to form a film, so as to obtain a film A; adding a phosphoric acid buffer solution, hydrating, incubating, and filtering to obtain a hydrated solution; s2-2, dissolving functionalized polyethylene glycol in a solvent, and rotating to form a film to obtain a film B; and hydrating the membrane B by using a hydration solution, and filtering to obtain the triptolide nano-micelle. The triptolide nano-micelle can be used for drug preparation through carrier structure innovation, drug synergy and targeted intelligent drug release, and realizes high-efficiency and low-toxicity treatment of rheumatoid arthritis.

The invention provides application of combination of amifostine and calycosin in preparation of a preparation for preventing and / or treating radiation-induced lung injury, and belongs to the technical field of medicines. According to the invention, the amifostine and the calycosin are combined for use, so that the medication side effect caused by independent use of the amifostine in the prior art is effectively solved, and the prevention effect and the treatment stability of the amifostine are improved. According to the pharmaceutical composition disclosed by the invention, the amifostine and the calycosin are combined for use, so that the pharmaceutical composition has a good prevention and treatment effect on radiation-induced lung injury caused by ionizing radiation, and adverse reactions such as hypotension and nausea generated when the amifostine is independently used cannot be generated. And when the administration dosage of the amifostine and the calycosin is low, the survival rate of a radiation-induced lung injury model mouse is remarkably improved, and no toxic or side effect exists.