Determining method for contents of twelve components in traditional Chinese medicine composition preparation

A measurement method and composition technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., can solve the problems of long inspection cycle and high cost

Active Publication Date: 2015-09-16
HEBEI YILING MEDICINE INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the large number of ingredients in the Chinese medicine composition, the current method has a long inspection period and high cost. Therefore, in order to effectively control the product quality and ensure the public's drug safety, this study uses UPLC- MS determination of calycosin-7-O-glucoside (1), isoquercitrin (2), naringerin (3), hesperidin (4), ginsenoside Re (5), Ginsenosides Rg1 (6), Ginsenosides (7), Ginsenosides Rf (8), Ginsenosides Rb1 (9), Astragaloside IV (10), Ginsenosides Rd (11), Ginsenosides H1 (12) content, to provide a reference for its quality control

Method used

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  • Determining method for contents of twelve components in traditional Chinese medicine composition preparation
  • Determining method for contents of twelve components in traditional Chinese medicine composition preparation
  • Determining method for contents of twelve components in traditional Chinese medicine composition preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: the preparation of medicine capsule of the present invention

[0056] prescription:

[0057] Astragalus 450g Aconite 112.5g Ginseng 225g Salvia miltiorrhiza 225g Tinglizi 150g

[0058] Xiangjia skin 180g Alisma 225g safflower 90g Polygonatum 75g Chenpi 75g

[0059] Preparation:

[0060] (1) Astragalus, Tinglizi, Alisma, ginseng, and Xiangjiapi were refluxed and extracted twice according to the above prescription with 8 times the amount (weight ratio) of 70% ethanol, the first time was 3 hours, the second time was 2 hours, and combined Extract the liquid, filter it, recover ethanol from the filtrate under reduced pressure, concentrate to 60°C and measure the clear paste with a relative density of 1.25-1.30, and set aside;

[0061] (2) Distill cinnamon sticks and tangerine peel to extract volatile oil according to the proportion, filter the aqueous solution after oil extraction, and set aside, add 8 times the amount of water to the residue and decoct for...

Embodiment 2

[0092] Embodiment 2: the preparation of pharmaceutical tablet of the present invention

[0093] prescription:

[0094] Astragalus 150g Aconite 40g Ginseng 225g Salvia 225g Tinglizi 50g Xiangjiapi 180g Alisma 75g Polygonatum 75g Guizhi 30g Safflower 90g Chenpi 25g

[0095] Preparation:

[0096] (1) Reflux extract astragalus, Tinglizi, Alisma, ginseng, and Xiangjia bark according to the above prescription plus 8 times the amount of 70% ethanol for 2 times, the first time for 3 hours, and the second time for 2 hours, combine the extracts, filter After filtration, the filtrate was decompressed to recover ethanol, concentrated to 60°C to measure the clear paste with a relative density of 1.25-1.30, and set aside;

[0097] (2) Distill cinnamon sticks and tangerine peel to extract volatile oil according to the prescription amount, filter the aqueous solution after oil extraction, and set aside, add 8 times the amount of water to the residue and decoct for 1 hour, filter, combine ...

Embodiment 3

[0114] Embodiment 3: the preparation of pharmaceutical granule of the present invention

[0115] prescription:

[0116] Astragalus 250g Aconite 112.5g Ginseng 200g Salvia 120g Tinglizi 135g

[0117] Xiangjiapi 150g Alisma 200g Polygonatum 60g Guizhi 75g Safflower 75g Chenpi 60g

[0118] Preparation method: Prepare granules according to conventional preparation methods.

[0119] The content assay method of this granule is by the following steps:

[0120] A, preparation of the test solution: take the Chinese medicine composition preparation, grind finely, accurately weigh 0.1g, place in a stoppered Erlenmeyer flask, add methanol 20mL, weigh it, let it cool for 10 minutes after ultrasonic treatment, and weigh , use methanol to make up for the lost weight, shake well, and pass through a 0.2 μm filter membrane;

[0121] B. Preparation of the standard curve: Accurately weigh the reference substances astragaloside IV, calycosin-7-O-glucoside, ginsenoside Rb1, ginsenoside Re, ...

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Abstract

The present invention discloses a UPLC-MS quantitative method for the contents of twelve components in a traditional Chinese medicine composition preparation, specifically determination of the contents of calycosin-7-O-beta-D-glucoside (1), isoquercitrin (2), narirutin (3), hesperidin (4), ginsenoside Re (5), ginsenoside Rg1(6), periplocoside (7), ginsenoside Rf (8), ginsenoside Rb1 (9), astragaloside (10), ginsenoside Rd (11) and periplocin H1 (12) in the traditional Chinese medicine composition, and belongs to the field of traditional Chinese medicine composition preparation component detection. The determining method of the present invention has characteristics of short period, good reproducibility and high sensitivity.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and in particular relates to a content determination method for each component in a traditional Chinese medicine preparation. Background technique [0002] The traditional Chinese medicine composition is a compound traditional Chinese medicine composed of 11 traditional Chinese medicines such as astragalus, ginseng, xiangjiapi, and cassia twig. , moderate congestive heart failure and other diseases. Modern studies have shown that the active ingredients in this traditional Chinese medicine composition are various, mainly including flavonoids, saponins, alkaloids, etc. At present, the research on this traditional Chinese medicine composition is mainly focused on clinical application and qualitative research on its chemical components. However, there are few reports on the quantitative analysis of its active ingredients. In recent years, liquid chromatography-mass spectrometry ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 刘敏彦赵韶华刘颖欧阳玥李松乔莉
Owner HEBEI YILING MEDICINE INST
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