23 results about "Ginsenoside Rd" patented technology

The invention discloses Dubosiella Newyorkens TZF-001 capable of producing beta-glucosidase and application of the Dubosiella Newyorkens TZF-001 in preparation of ginsenoside through fermentation and conversion, and belongs to the field of probiotic fermentation. The Dubosiella Newyorkens TZF-001 is preserved in the China Center for Type Culture Collection on December 15, 2025, and the preservation number of the Dubosiella Newyorkens TZF-001 is CCTCC (China Center for Type Culture Collection) NO: M20252889. According to the invention, ginsenoside Rb1 is used as a raw material, and Dubosiella Newyorkens TZF-001 with high yield of beta-glucosidase is used for fermentation and conversion of ginsenoside so as to prepare ginsenoside Rd. The ginsenoside Rb1 can be completely converted into Rd after fermentation, the dependence of a traditional method on acid-base hydrolysis, high temperature and high pressure and steam treatment is avoided, the reaction condition is mild, and the method has the advantages of being environmentally friendly, low in cost, high in product purity and the like. The method opens up a brand new technical path for green and low-cost preparation of ginsenoside Rd, and has remarkable innovativeness and popularization value in the field of industrialization of active ingredients of natural medicines.

The present application relates to a ginsenoside composition for inhibiting tyrosinase activity, which comprises any two or any three of ginsenoside Rd, ginsenoside Re or ginsenoside Rf. Ginsenoside Rf can be used as a synergistic molecule of ginsenoside Rd and Re, and can significantly enhance the inhibitory ability of tyrosinase activity through synergistic effect, thereby reducing the synthesis and secretion of melanin.

The application discloses a composition containing burdock polysaccharide for reducing blood sugar of type 2 diabetes patients and a preparation method and application thereof, and belongs to the technical field of medicine and food. The composition contains burdock polysaccharide, chitooligosaccharide, chlorogenic acid, epicatechin, resveratrol, ginsenoside Rd, and vitamin D, vitamin B1, vitamin B6, vitamin B12, magnesium glycinate and thaumatin protein. The application takes burdock polysaccharide as a core long-acting component, takes chitooligosaccharide as a short-acting synergistic component, constructs a long-short-acting synergistic blood sugar reducing system, and realizes the organic combination of rapid blood sugar reduction and stable blood sugar control. Experiments show that the composition can significantly reduce the fasting blood sugar of type 2 diabetes model rats, improve insulin resistance and glucose tolerance, reduce blood lipid and inflammatory factor levels, and reduce oxidative stress damage. The alpha-glucosidase inhibitory activity and in-vivo comprehensive blood sugar reducing effect of the composition are better than those of single burdock polysaccharide, and the composition has good taste and is suitable for long-term use of diabetes patients.

The application discloses a kind of β-glucosidase GE000505 for saponin conversion and its preparation method and application, belong to biotechnology field, the amino acid sequence of the β-glucosidase GE000505 as shown in SEQ ID NO.1, it is encoded by the gene with nucleotide sequence as shown in SEQ ID NO.2.This β-glucosidase GE000505 of the application can convert high content ginseng and sanchi saponin into rare saponin R2, Rg3, Rg2 and Rh1, can convert sanchi saponin R1 into R2 entirely, convert ginsenoside Rd into Rg3 entirely, convert ginsenoside Re into Rg2 entirely, convert ginsenoside Rg1 into Rh1 entirely, convert ginsenoside F1 into aPPT entirely, convert ginsenoside F2 into aPPD entirely, solve the problem that existing β-glucosidase is not resistant to alcohol in the conversion of ginsenoside.

The present application relates to the technical field of compound analysis, and in particular to a quality analysis method of ginseng medicinal materials, comprising the following steps: S100, preparation of a control solution: taking ginsenoside Rg1 control product, ginsenoside Re control product, ginsenoside Rf control product, ginsenoside Rb1 control product, ginsenoside Rc control product, ginsenoside Rb2 control product, ginsenoside Rd control product, 3-O-feruloylquinic acid control product, 4-coumaric acid control product, 8-O-4 / 8-O-4 dehydrogenated tri-ferulic acid control product, and adding methanol to prepare a mixed solution, and shaking to obtain the control solution. The present application synchronously monitors two components of saponins and phenolic acids under one detection method, monitors ten component contents of seven saponin components and three polyphenol components, obtains characteristic spectra of saponin components and polyphenol components, and the monitoring quality is more comprehensive and efficient.

The invention discloses a construction method of a finger-print spectrum of a styptic capsule and application of the styptic capsule in quality detection, and belongs to the field of Chinese patent medicine detection. The method comprises the following steps: firstly, adding 80% methanol solution into contents of different batches of Xuezhenning capsules for extraction to prepare Xuezhenning capsule test solution; the method comprises the following steps: taking ginsenoside Rg1, notoginsenoside R2, ginsenoside Rd, caffeic acid, isorhamnetin, verbascoside, aesculetin, ferulic acid, rutin and quercetin as reference substances, and preparing a reference substance solution; respectively injecting the test solution and the reference solution into a high performance liquid chromatograph to obtain corresponding chromatograms; performing similarity analysis on the chromatogram of the test solution; and finally, according to the chromatogram of the test solution and the chromatogram of the reference substance solution, combining a total ion chromatogram obtained by the high-resolution mass spectrum and a mass spectrum result map of the chemical components to construct the fingerprint spectrum of the styptic Ning capsule. The method can be used for simultaneously detecting 10 components in the haemostatic Ning capsule, and can be used for accurately, clearly and objectively evaluating the quality of the haemostatic Ning capsule.

The invention discloses a method for preparing ginsenoside Rd through catalysis of L-arabinopyranosidase, which comprises the following steps: taking ginsenoside Rb1 as a substrate, and catalyzing the substrate to be hydrolyzed by using the L-arabinopyranosidase (Uniprot ID (Identity) accession number A0A6I4WC78) from Actinomadura rayonensis) or a mutant of the L-arabinopyranosidase so as to obtain the ginsenoside Rd. According to the method, the high-concentration ginsenoside Rb1 can be efficiently converted under the condition of high-concentration methanol, and industrial production of ginsenoside Rd is promoted.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a construction method of a characteristic spectrum of a traditional Chinese medicine compound preparation for treating senile dementia. The method is creatively characterized in that detection of characteristic peaks is realized through extraction, preparation and purification of an extract and specific gradient selection under a specific mobile phase condition. According to the method, seven chemical components in the traditional Chinese medicine compound preparation can be analyzed, seven characteristic peaks including epimedin C, ginsenoside Rd, pachymic acid, dehydrotumuloic acid, alpha-asarone, polygala mountain ketone III and tenuigenin are identified, verification research on specificity, precision, repeatability and stability is carried out on the method, and it is proved that the method is accurate and reliable. The technical means is used as a method for overall evaluation of the traditional Chinese medicine compound preparation, and a quality control system of the traditional Chinese medicine compound preparation is comprehensively improved.

The application provides a multi-component quantitative analysis method of anti-aging tablets, acetonitrile-0.1% formic acid aqueous solution is used as a mobile phase, an HSS T3 chromatographic column is used for target compound separation, and 13 active components in the anti-aging tablets are simultaneously determined in an MRM mode by using an electrospray ionization triple quadrupole mass spectrometer, the linear range of the 13 active components is 0.4-100 ng / mL, the linear range of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf and ginsenoside Rg1 is 4-1000 ng / mL, the method has high sensitivity, strong specificity and good reproducibility, and realizes simultaneous quantitative analysis of the 13 active components in the anti-aging tablets.

The invention belongs to the technical field of microbial bioconversion, and particularly relates to application of Burkholderia sp. WJ-02 in ginsenoside conversion and a conversion method, and the Burkholderia sp. WJ-02 can convert ginsenoside Rb3 into ginsenoside Rd and rare ginsenoside F2. The Burkholderia sp. WJ-02 is separated from rhizosphere soil of American ginseng, the Burkholderia sp. WJ-02 can efficiently and selectively convert main ginsenoside Rb3 into ginsenoside Rd and rare ginsenoside F2 by taking American ginseng stem and leaf saponin as a substrate, and conversion target products are concentrated, so that subsequent purification is facilitated.

The application discloses a plant lactobacillus gasseri strain capable of transforming ginsenoside and cassia glycoside, and application of the strain in lipid reduction and alleviation of fatty liver, and belongs to the fields of microbial technology, medicine and functional food. The core feature of the strain is that the strain can not only transform ginsenoside Rb1 into ginsenoside Rd with higher activity, but also transform cassia glycoside into cassia glycoside base, thereby improving the efficacy. The animal experiment proves that the strain and the corresponding substrate are combined to play a better role in lipid reduction and liver protection: the serum total cholesterol and triglyceride levels of high-fat diet mice can be effectively reduced, and the liver weight, liver lipid deposition and liver tissue fatty degeneration can be reduced and improved. The strain provides a new core strain and technical scheme for developing microecological preparations for preventing and / or assisting in treating hyperlipidemia, non-alcoholic fatty liver and other metabolic diseases.

The invention discloses a compound melanterite pill specific chromatogram and a construction method and a quality detection method thereof, and belongs to the field of Chinese patent medicine detection. Taking different batches of compound melanterite pills as test articles to prepare a compound melanterite pill test article solution; the method comprises the following steps: taking gallic acid, ginsenoside Rg1, procyanidine B2, cinnamic acid, coumarin, ginsenoside Rh1, cinnamyl alcohol, cinnamaldehyde, arachidonic acid, epicatechin, 2-methoxycinnamic acid, ginsenoside Rb1, ginsenoside Rd, betulinic acid and 5-hydroxymethylfurfural as reference substances to prepare a single reference substance solution; performing chromatographic analysis on the compound melanterite pill test solution and the single reference substance solution, and recording chromatograms; carrying out similarity analysis; and constructing the characteristic chromatogram of the compound melanterite pill according to the chromatogram of the test solution of the compound melanterite pill and the chromatogram of the single reference substance solution in combination with the total ion chromatogram obtained by the high-resolution mass spectrum and the mass spectrum result map of the chemical components. The method can accurately, clearly and objectively evaluate the quality of the compound melanterite pill.

A strain of *Duboscirella nystatin* that produces β-glucosidase ( Dubosiella newyorkensis TZF-001 and its application in the fermentation and conversion of ginsenosides belong to the field of probiotic fermentation. This *Duboscirella nystatin* (TZF-001) and its application in the fermentation and conversion of ginsenosides belong to the field of probiotic fermentation. Dubosiella newyorkensis TZF-001 was deposited at the China Center for Type Culture Collection on December 15, 2025, with accession number CCTCC NO: M20252889. This invention uses ginsenoside Rb1 as a raw material and utilizes *Duboscirella nystatin*, a bacterium that produces high levels of β-glucosidase. Dubosiella newyorkensis The TZF-001 fermentation method converts ginsenosides (Rb1) to ginsenoside Rd. After fermentation, ginsenoside Rb1 can be completely converted to Rd. This invention avoids the dependence on acid-base hydrolysis, high temperature and pressure, and steam treatment in traditional methods. Its reaction conditions are mild, and it boasts advantages such as environmental friendliness, low cost, and high product purity. This opens up a new technological path for the green and low-cost preparation of ginsenoside Rd, and has significant innovative and promotional value in the industrialization of natural drug active ingredients.

The invention discloses a beta-glucosidase BglSK mutant and an application of the beta-glucosidase BglSK mutant. The BglSK mutant is mutated by taking an amino acid sequence as shown in SEQ ID NO. 1 as a benchmark. According to the beta-glucosidase BglSK mutant disclosed by the invention, one or more amino acid residues are introduced at a specific site for substitution, so that the thermal stability and / or catalytic activity of beta-glucosidase are / is remarkably improved; in addition, ginsenoside Rd or analogues thereof can be efficiently catalyzed, and rare ginsenoside F2 and / or rare ginsenoside CK can be directionally generated. According to the beta-glucosidase BglSK mutant disclosed by the invention, large-scale production of rare ginsenoside is successfully realized, and the beta-glucosidase BglSK mutant has huge industrial application prospect and value.

The invention belongs to the technical field of enzyme engineering, and discloses a fusion enzyme for producing rare ginsenoside Rh2 and a preparation method and application thereof. The active part of the fusion enzyme is obtained by sequentially connecting beta-glucosidase Bglpm, a connecting peptide and beta-glucosidase Bgp1. Two enzymes are fused into one enzyme molecule through a gene fusion technology, and the fusion enzyme obtained after screening and optimization effectively improves the catalytic efficiency of the enzyme, so that the reaction rate of converting ginsenoside Rd into rare ginsenoside Rh2 is remarkably improved, the fusion enzyme simplifies the catalytic process, reduces the reaction steps and reaction time, and is suitable for industrial production. And the production efficiency can be improved and the production cost can be reduced.

The application belongs to the technical field of microbial bioconversion, and particularly relates to application of Burkholderia WJ-02 in conversion of ginsenoside and a conversion method, wherein the Burkholderia WJ-02 can convert ginsenoside Rb3 into ginsenoside Rd and rare ginsenoside F2. The Burkholderia WJ-02 is obtained by being separated from rhizosphere soil of American ginseng, the strain can efficiently and selectively convert ginsenoside Rb3 into ginsenoside Rd and rare ginsenoside F2 with American ginseng stem and leaf saponin as a substrate, and the conversion target product is concentrated, so that subsequent purification is facilitated.

The application discloses a beta-glucosidase BglSK mutant and application thereof. The BglSK mutant is mutated based on the amino acid sequence shown in SEQ ID NO. 1. The beta-glucosidase BglSK mutant of the application significantly improves the thermal stability and / or catalytic activity of the beta-glucosidase by introducing one or more amino acid residue substitutions at specific sites. In addition, the beta-glucosidase BglSK mutant can efficiently catalyze ginsenoside Rd or an analogue thereof to direct generation of rare ginsenoside F2 and / or rare ginsenoside CK. The beta-glucosidase BglSK mutant of the application successfully realizes large-scale production of rare ginsenosides, and has great industrial application prospect and value.

The application discloses an Allen's total ginsenoside atomizing eye drop and a preparation process thereof, and relates to the technical field of eye drop preparation. The eye drop comprises 60-70% of total ginsenoside extracted from panax notoginseng leaves, 30-50% of total ginsenoside extracted from panax notoginseng flowers, 55-65% of total ginsenoside extracted from panax notoginseng roots and 5-10% of water-extracted total ginsenoside of panax notoginseng. The total ginsenoside extracted from panax notoginseng leaves is composed of ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd. In the application, the Allen's total ginsenoside atomizing eye drop prepared by the process of S1-S4 is used, so that the ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd in the eye drop are not lost, the quality of the prepared eye drop is improved, and the relief effect of eye dryness is effectively improved.

A strain of Bacteroides monomorpha SJY-001 producing β-glucosidase and its application in the fermentation and conversion of ginsenosides belong to the field of probiotic fermentation. This strain was deposited at the China Center for Type Culture Collection on March 19, 2026, with accession number CCTCC NO: M2026473. This technology uses ginsenoside Rb1 as raw material and utilizes a high-β-glucosidase-producing strain for fermentation transformation to first obtain primary ginsenoside Rd, which is then further directionally transformed into highly active rare ginsenosides (S)-Rg3 and Rg5, achieving multi-stage continuous directional biotransformation. This method abandons the traditional high-pressure processes such as strong acid and strong alkali hydrolysis, high temperature and high pressure, and has the advantages of mild reaction conditions, environmental friendliness, low preparation cost, high product purity and structural stability. It provides a safe, efficient, green and low-cost new preparation route for ginsenoside Rd and rare ginsenosides (S)-Rg3 and Rg5, and has outstanding innovation and broad prospects for promotion in the industrial production and high-value application of natural drug active ingredients.

This invention belongs to the field of thin-layer chromatography (TLC) identification technology for traditional Chinese medicine, and specifically relates to a rapid TLC identification method and application for American ginseng and ginseng. The TLC identification method includes the following steps: taking a test sample, adding a solvent, sonicating, and taking the supernatant as the test solution; taking American ginseng and ginseng reference materials, adding solvents respectively, sonicating, and taking the supernatant as American ginseng reference material solutions and ginseng reference material solutions; taking ginsenoside Rg1, ginsenoside Rf, ginsenoside Re, ginsenoside Rd, and pseudoginsenoside F11 reference standards, adding solvents, mixing, and obtaining reference solution; finally, performing TLC analysis and result determination on the test solution, reference material solution, and reference solution. This invention is simple to operate, highly efficient and rapid, has good repeatability and high accuracy, and is low in toxicity and durable, and can simultaneously detect adulteration, substitution, and confusion in American ginseng and ginseng.

The invention provides a fingerprint spectrum detection method of a heart-stabilizing traditional Chinese medicine composition, the detection method comprises a fingerprint spectrum and an effective index component determination method, a characteristic spectrum is established by adopting a high performance liquid chromatography-mass spectrometry method, and meanwhile, the effective index component determination method is used for determining the effective index component of the heart-stabilizing traditional Chinese medicine composition. Chemical characteristic index components in rhizoma nardostachyos, pseudo-ginseng, codonopsis pilosula and rhizoma polygonati medicinal materials in the heart-stabilizing granules are determined. According to the present invention, 22 batches of the heart stabilizing particles are determined, 8 index components such as polygonatum sibiricum alkali A, lobetyolin, desoxynardostinol A, notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd are identified, and the detection method has characteristics of rapid detection (detection time is less than 18 min), relatively comprehensive qualitative detection index relative components, high sensitivity, high accuracy, and the like. The internal quality of the compound traditional Chinese medicine composition can be objectively represented.

The invention discloses a method for preparing an extract rich in ginsenoside Rd by fermenting pseudo-ginseng with paecilomyces farinosus, and belongs to the technical field of microbial fermentation. The method specifically comprises the following steps: crushing and sieving pseudo-ginseng main roots, pseudo-ginseng incisions and pseudo-ginseng stems and leaves, collecting powder with different granularities, and mixing the powder with different granularities to obtain pseudo-ginseng main root coarse powder, pseudo-ginseng incisions coarse powder and pseudo-ginseng stem and leaf coarse powder; preparing pseudo-ginseng powder; preparing a pseudo-ginseng culture medium; the preparation method comprises the following steps: co-culturing paecilomyces farinosus and a pseudo-ginseng culture medium to obtain a fermented suspension culture; the preparation method comprises the following steps: dissolving panax notoginseng into a suspension culture, evaporating the suspension culture to remove water to obtain panax notoginseng plastid, performing reflux extraction on the panax notoginseng plastid, concentrating an extracting solution to obtain extract, dissolving the extract with water, adsorbing an aqueous solution of the extract into resin, eluting, concentrating an eluent, and drying to obtain the extract rich in ginsenoside Rd. The method disclosed by the invention has the advantages of simplicity in operation, no environmental pollution and high efficiency.

The invention provides a method for determining the content of multiple components in an Aidi preparation, and belongs to the technical field of medicines. The Aidi preparation is prepared from traditional Chinese medicinal materials including ginseng, cantharides, astragalus membranaceus and acanthopanax. The content determination method comprises the following steps: simultaneously determining cantharidin, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rd, isofraxidin, syringin, formononetin, astragaloside, chlorogenic acid, eleutheroside E, calycosin-7-glucoside, astragaloside II, neochlorogenic acid, cryptochlorogenic acid and eleutheroside E1 in the Aidi preparation by adopting liquid chromatography-mass spectrometry; the composition is prepared from effective components such as ginsenoside F3, ginsenoside Rg2, azelaic acid and the like. Compared with the prior art, the method has the advantages that the contents of the 20 effective components in the Aidi preparation are simultaneously measured under the same chromatographic condition through liquid chromatography-mass spectrometry, the method is convenient and rapid, the requirement for product quality detection is greatly improved, the method is more suitable for quality control of drugs in the future, and the drug quality and the clinical drug effect are guaranteed.