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Method for selectively producing ginsenoside rd from saponins of ginseng through enzymatic method

A ginsenoside and ginseng technology are applied in the field of selectively preparing ginsenoside Rd from ginsenoside by an enzymatic method, which can solve the problems of ginsenoside only increasing the price, restricting the large-scale use of ginsenoside, consuming cost, time and the like.

Active Publication Date: 2018-06-08
AMOREPACIFIC CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, in order to obtain pure specific ginsenosides in this existing method, the refining process requires additional costs, time, etc., resulting in an increase in the price of ginsenosides, which limits the large-scale use of ginsenosides in related products
[0009] In addition, if the enzyme reacts non-specifically, it will be converted into ginsenoside F2 and K compounds due to the non-selective removal of sugar groups in ginsenoside Rd, making it difficult to obtain intermediate metabolites including ginsenoside Rd with high yield

Method used

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  • Method for selectively producing ginsenoside rd from saponins of ginseng through enzymatic method
  • Method for selectively producing ginsenoside rd from saponins of ginseng through enzymatic method
  • Method for selectively producing ginsenoside rd from saponins of ginseng through enzymatic method

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reference example 1

[0034] [Reference Example 1] Preparation of Refined Ginseng Saponins

[0035] Put 2kg of red ginseng, white ginseng, fresh ginseng, ginseng whiskers or their ginseng leaves, ginseng flowers, and ginseng fruits into 20L of ethanol, reflux extraction for 3 times, and settle at 15°C for 6 days. Afterwards, the residue and filtrate were separated by filter cloth filtration and centrifugation, the concentrated solution obtained by concentrating the separated filtrate under reduced pressure was suspended in water, extracted 5 times with 1L ether to remove the pigment, and the water layer was extracted with 1L of 1 - Butanol extraction 3 times. All the 1-butanol layers thus obtained were treated with 5% KOH, washed with distilled water, and then concentrated under reduced pressure to obtain a 1-butanol concentrate, which was dissolved in a small amount of methanol, and then a large amount of ethyl acetate was added Esters, by drying the precipitate generated, 40-80g of refined ginse...

Embodiment 1

[0036] [Example 1] Preparation of Ginsenoside Rd by Enzyme Reaction

[0037] Dissolve 10 g of the refined ginsenosides (containing ginsenosides Rb1, Rb2, Rc, Rd, Re, Rg1, Rf, etc.) in Reference Example 1 above in 1 L of water.

[0038] Then, 200% by weight of the pectinase isolated from Trichoderma reesei was added to the above-mentioned mixed liquid, and then reacted at 30° C. for 48 hours. Periodically confirm the complete consumption of the substrate by thin-layer chromatography, inactivate the enzyme by heating in a boiling water bath for 10 minutes, and terminate the reaction. Finally, add ethyl acetate or ethanol to the reaction solution at a ratio of 1:1 (relative to the volume ratio of the reaction solution), extract 3 times, concentrate, and separate by silica gel column chromatography (chloroform:methanol=9:1). Ginsenoside Rd( figure 1 ).

[0039] In 10 g of refined ginsenosides used there were: 2.66 g of ginsenoside Rb1, 0.73 g of ginsenoside Rb2, 1.23 g of ginse...

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Abstract

The present invention relates to a method for selectively producing ginsenoside Rd, which is originally present in a trace amount in ginseng, from panaxadiol-based saponins in ginseng and, more specifically, to a method capable of obtaining a desired target compound, that is, ginsenoside Rd, in high yield, by treating panaxadiol-based saponins, obtained from ginseng, with particular enzymes to structurally convert the saponins.

Description

technical field [0001] The present invention relates to a method for selectively preparing ginsenoside Rd which originally exists in a small amount in ginseng from ginsenoside Rb1 which exists in a large amount in ginseng. Saponin Rb1 is treated to convert the structure of the saponin to obtain the target compound, that is, a method capable of obtaining ginsenoside Rd in high yield. Background technique [0002] Ginsenoside has a specific chemical structure different from saponins found in other plants, so it has unique pharmacological properties. It is also called "ginsenoside" because of the meaning of glycoside (glycoside) in ginseng. The specific types of ginsenosides include: panaxadiol series containing ginsenosides Rb1, Rb2, Rc, Rd, K compounds, Mc compounds and O compounds, etc. and ginsenosides Re, Rf, Rg1, Rg3, Rg5, Rh1 and Rh2, etc. Panaxatriol series, these ginsenosides show different efficacy. [0003] [Reaction 1] [0004] [0005] As shown in the above r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44C12P33/16C12N9/42C07H17/00C07J17/00
CPCC07H17/00C07J17/00C12P19/44C12P33/16C12Y302/01004
Inventor 南基帛金东泫洪勇德朴浚星
Owner AMOREPACIFIC CORP
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