115 results about "Trace Amounts" patented technology

The invention discloses a sweat in-situ detection sensor based on a microneedle patch and a preparation method of the sweat in-situ detection sensor, and belongs to the technical field of sweat detection. The sweat in-situ detection sensor comprises a flexible substrate, and the flexible substrate comprises a hydrophobic layer used for being in contact with skin; the hydrophilic layer is arranged on the hydrophobic layer, and the sweat contact angle of the hydrophilic layer is smaller than that of the hydrophobic layer; the micro-channel layer comprises a micro-needle patch, and the micro-needle patch is arranged on the side, away from the hydrophobic layer, of the hydrophilic layer; the aggregation layer is arranged on the side, away from the hydrophilic layer, of the microneedle patch; the detection electrode is arranged between the aggregation layer and the microneedle patch; the volatilization layer is arranged on the side, away from the microneedle patch, of the aggregation layer, the problems that according to an existing wearable sweat detection sensor, the sweat utilization rate is not saturated, sweat is accumulated and flows back, a trace amount of sweat is difficult to fully utilize, and the detection precision of physiological signals is affected can be solved, and the application potential is huge.

The invention belongs to the technical field of medicines, and particularly discloses a biomarker for detecting kidney fibrosis related diseases and application of the biomarker. The biomarker comprises serum copper cyanin, or the serum copper cyanin and serum creatinine. Compared with other methods for diagnosing renal fibrosis, the serum cupreuloplasmin has the following three advantages: 1, compared with a conventional diagnostic marker (such as serum creatinine), the cupreuloplasmin can detect up-regulation in the serum of a patient suffering from early chronic kidney diseases, and can be combined with an existing mature diagnostic marker for detection; secondly, compared with renal tissue pathologic biopsy, the use of the copper cyanin as the diagnostic marker has non-invasiveness, and only a small amount or even a trace amount of patient serum is needed, so that the harm to the body health of the patient is avoided; and thirdly, compared with a nuclear magnetic resonance method for analyzing the metabolic components of the urine, the method for detecting the markers in the serum is lower in cost, more flexible, high in timeliness and easier to popularize.

The invention relates to the field of drug analysis, and discloses a trace analysis method and system for antiviral drugs in sewage based on LC-MS / MS. The method comprises the following steps: filtering a sewage sample, analyzing the drug properties of the antiviral drugs, carrying out solid-phase extraction on the filtered sewage sample, preparing an LC-MS / MS module of the extracted sample, determining a mobile phase of a chromatographic column, and determining the mobile phase of the chromatographic column. Balancing the chromatographic column, constructing chromatographic conditions for balancing the chromatographic column, determining a tuning solution and a mass axis calibration compound of a mass spectrometer, calibrating the mass spectrometer, and constructing mass spectrum conditions of the calibrated mass spectrometer; the method comprises the following steps: preparing a standard solution containing an antiviral drug, analyzing the standard solution, constructing a standard curve of the standard solution, and calculating a response coefficient of an LC-MS / MS module; and analyzing the extracted sample to obtain a sample mass spectrum and a sample chromatogram, and calculating the content of the antiviral drug. According to the method, the sensitivity and accuracy of trace analysis of the antiviral drugs in the sewage can be improved.

The augmented-reality automated allergy office method comprises initially delivering a trace amount of a first allergen to a first test site via a multiple test applicator as the multiple test applicator delivers a trace amount of a second allergen to a second test site during allergen deposition. The multiple test applicator is cooperatively engageable with a fluid tray during allergen loading and with the patient's skin during allergen deposition. Then, capturing a first image of the first and second test site using a smart phone or tablet computer. Subsequently, capturing a second image of the first and second test site within 15 to 20 minutes after allergen deposition. And finally, developing a computer-generated treatment plan using machine learning for the patient with an allergy condition that is a step-by-step procedure, the computer-generated treatment plan being stored in a medical database for treating patients with the allergy condition.

This invention discloses a rapid mass spectrometry method and kit for the simultaneous detection of multiple components in body fluids, belonging to the field of biological detection and identification technology. It can rapidly detect trace compounds such as drugs and biomarkers in complex biological samples. This kit mainly utilizes loaded functional materials to achieve extraction and enrichment of target analytes and is equipped with a quick connector for coupling with a detector to achieve rapid detection. Compared with traditional methods, the significant advantages of the method established by this kit are that it does not require complex sample pretreatment, allows for micro-quantitative sampling, requires small sample volumes, facilitates convenient storage of biological samples, is easy to operate, does not require specialized technical personnel, and enables rapid on-site detection when coupled with portable mass spectrometry. This kit has been successfully used for the detection of quinine, procainamide hydrochloride, atenolol, and nortriptyline in complex samples such as blood and serum, and has significant clinical and economic value.

The utility model provides a disposable needleless injection needle which comprises a pressure source instrument connecting part, a liquid medicine injection part, a liquid medicine storage part and a liquid medicine injection part, and a screw sequentially penetrates through a screw hole of the liquid medicine injection part and a screw hole of the liquid medicine storage part and finally enters a threaded hole of the pressure source instrument connecting part to achieve overall fixation. The side wall of the liquid medicine injection part and the side wall of the liquid medicine storage part are fixed through screws, the pressure source instrument connecting part is connected with an external micro-jet injector to obtain a pressure source, and the liquid medicine injection part is connected with an external injection tube to obtain liquid medicine. And the continuous broken injection of the liquid medicine is realized. The utility model has the beneficial effects that a trace amount of medicine can be quickly and accurately injected into the skin surface layer, so that pain and recovery time are reduced; under the condition that the needle head is not replaced, liquid medicine can be added at any time, and convenience and rapidness are achieved.

The invention discloses a double-layer detachable purification and enrichment device and a use method thereof, and relates to the technical field of sample analysis. The device comprises an enrichment column, a purification column and a needle head, the enriching column is detachably connected with the purifying column; as required, the needle head is detachably connected with the enriching column or the purifying column; wherein the enrichment column is in an injector shape, and a piston, a first filter disc, an enrichment material and a second filter disc are sequentially arranged in a cavity in the enrichment column from top to bottom; the piston moves up and down in the cavity, the first filter disc, the enrichment material and the second filter disc are arranged at the bottom in the cavity, and the enrichment material is filled in an interlayer between the first filter disc and the second filter disc and is fixed; a third filter disc, a purification material and a fourth filter disc are arranged in the purification column; and the purification material is filled in an interlayer between the third filter disc and the fourth filter disc and is fixed. The device integrates the processes of sampling, purification, enrichment, elution, sample loading and the like, and can realize rapid purification, enrichment and elution of trace target objects in a solution sample.

The utility model discloses a portable traditional Chinese medicine olfactory diagnosis instrument, which comprises a sample collection device, a gas enrichment device, a mass spectrometer, a data processor and a display, the mass spectrometer is sequentially connected with the data processor and the display, the gas enrichment device is test paper with an enrichment reagent encapsulating nano-metal particles, the enrichment efficiency is effectively improved, and the detection accuracy is improved. Therefore, the detection sensitivity and accuracy of the mass spectrometer are improved; the device is provided with the ion trap mass spectrometer, has the characteristics of high detection speed, high resolution and high sensitivity, and has performance advantages in qualitative and trace analysis aspects; and due to small volume and strong portability, the device can be conveniently integrated into portable or field detection equipment, can be used for auxiliary diagnosis of heart failure and asthma, and also has great clinical application value in the aspects of disease evolution and disease prognosis evaluation.

The utility model relates to an adsorption device for simply measuring gaseous iodine, which comprises a large glass bottle and a small glass bottle, the upper ends of the large glass bottle and the small glass bottle are open, the large glass bottle serves as a container for bearing solid iodine, iodine adsorption materials are placed in the small glass bottle, the small glass bottle is placed in the large glass bottle through a support structure arranged in the large glass bottle, and the upper surface of the support structure is a plane. The support structure separates the small glass bottle from the large glass bottle, and the top of the small glass bottle is not in contact with the bottom of the large glass bottle cap. According to the utility model, through the measures, experiments or operations which may have risks when contact time between experimenters and instruments is reduced are realized, and the safety of operators is ensured; the adsorption effect of the measurement material is enhanced, the test time is shortened, the accuracy, precision and sensitivity of weighing analysis are improved, and the method is greatly helpful for analysis of trace samples and trace components.

The present invention relates to an equal-dose opposed-vessel monitoring pulmonary artery-pulmonary vein CT angiography method and system. For the method, the dose of the contrast agent bolus test, the flow rate and volume of the contrast agent, and the flow rate and volume of the normal saline are all consistent with those in the formal scan; during the bolus test, it is confirmed that a small amount of contrast agent flows into the pulmonary vein of the monitored slice while the pulmonary artery is filled, so as to obtain the optimal delay time of pulmonary artery CTA; it is confirmed that almost all of the contrast agent in the monitored slice has flowed through the pulmonary artery, and there is a trace amount of contrast agent at the end of the pulmonary artery near the periphery, so as to obtain the optimal delay time of pulmonary vein CTV. The system includes each module for realizing the above functions. The present invention can achieve accurate timing of pulmonary artery CTA and pulmonary vein left atrial CTV scans, and can also achieve accurate timing of combined one-stop right atrium ventricle pulmonary artery and pulmonary vein left atrium ventricle combined scans, realizing precise diagnosis and treatment.

The invention discloses a magnetic multi-walled carbon nanotube material for enriching and synthesizing cannabinoid drugs as well as a preparation method and application of the magnetic multi-walled carbon nanotube material, the magnetic multi-walled carbon nanotube material is of a core-shell structure, Fe3O4 wrapping carboxylated multi-walled carbon nanotubes is taken as a central core, and the central core is externally coated with a load layer composed of dopamine and chitosan; the magnetic multi-walled carbon nanotube material provides a novel functional platform for solid-phase extraction detection of trace synthetic cannabinoid drugs due to a multi-action site synergistic mechanism and a simple magnetic separation characteristic, and is particularly suitable for high-throughput sample pretreatment in sewage toxicity monitoring; the method is simple and convenient in the process of treating trace synthetic cannabinoid drugs in a complex matrix sample, greatly improves the detection sensitivity of an instrument, can be matched with an on-site rapid detection instrument or a laboratory large-scale analysis instrument for use, reduces pretreatment steps, avoids the defects of complex steps and low repeatability in the prior art, and has a great application prospect.

This invention discloses a method for enhancing the terahertz absorption spectrum of materials. The method uses a terahertz time-domain spectroscopy system to measure the material spectrum, calculates the material's transfer function, amplitude, and phase using the acquired reference signal and sample signal, fits the material's absorption spectrum by constructing a multi-Gaussian function model, and enhances the absorption spectrum through a coefficient enhancement factor. This strengthens the ability to identify minute differences in the terahertz spectrum and improves the ability of terahertz spectroscopy to distinguish substances with similar properties, similar compositions, and trace amounts.

The application discloses a raw material medicine trace heavy metal ion detection method and system based on a micro-fluidic chip, relates to the technical field of micro-fluidic detection and electrochemical analysis, and comprises the following steps: weighing, constant volume, clarification of the raw material medicine sample, solidifying target ions, standard calibration and peak interval / scanning parameters into a detection parameter table; according to the detection parameter table, the flow and valve control are controlled to complete liquid preparation, muSPE enrichment, washing, elution and constant volume loading on the chip; blank, no standard addition, one-time standard addition and numbered archiving are sequentially measured; baseline is made according to the solidified parameters, integration and blank deduction are carried out, the solution concentration is calculated combined with standard addition measurement, and the content is output. The method disclosed by the application can constrain micro-fluidic liquid preparation, enrichment, elution and constant volume loading, so that blank, no standard addition and standard addition three rounds are measured under the same source condition; the curve and standard addition measurement are numbered and archived, integral, blank deduction and concentration are solved simultaneously, and the quantitative consistency, traceability and calculation reliability are improved.

The invention discloses a quantitative method for detecting trace free chelate deferoxamine in ACCs medicine based on reversed phase chromatography. The quantitative method comprises the following steps: preparing a deferoxamine standard substance solution; the method comprises the following steps: respectively detecting a sample to be detected and a deferoxamine standard substance solution by adopting a liquid chromatograph under the detection conditions that a chromatographic column is PLRP-S (2.1 * 100 mm, 5 [mu] m), the column temperature is 25-80 DEG C, and a filler is PS / DVB porous particles; mobile phases: a phase A is an aqueous solution containing organic acid, and a phase B is an organic solvent containing organic acid; establishing a calibration curve according to the concentration of the standard deferoxamine solution and the peak area of the corresponding elution peak, and then substituting the peak area of the elution peak of the sample to be detected into the calibration curve to obtain the concentration of the deferoxamine in the sample to be detected. According to the method, the tedious sample pretreatment step is omitted, the direct sample injection analysis of the sample is realized, the experimental period is greatly shortened, and the detection efficiency is improved.

To address the difficulties of collecting sebum gas from the human body and its low analysis accuracy, the present invention discloses a device for collecting sebum gas and enhancing its infrared spectrum. By combining a micro-electromechanical system (MEMS), functional two-dimensional materials, and metal surface plasmon resonance (MSPR) technology, the present invention produces a device similar in shape and size to a conventional Band-Aid. By applying the device to the human body for 3-5 minutes, trace amounts of sebum gas can be collected. Furthermore, the device can enhance the weak infrared spectrum of sebum gas, further improving the recognition of the sebum gas infrared spectrum, providing a simple, efficient, and accurate device for diagnosing diseases based on sebum gas.

The present invention focuses on a surface-enhanced Raman nanoparticle and its application in detecting fentanyls, within the realm of drug safety assessment. The surface-enhanced Raman nanoparticle comprises a noble metal, with gold nanocakes being the preferred choice. The detection method for fentanyls employs the surface-enhanced Raman nanoparticle described in the present invention to conduct Raman detection. This nanoparticle and the associated detection method enable the rapid and non-destructive identification of trace amounts of fentanyls in the target sample, presenting significant potential for application in public safety detection.

The invention discloses a rapid detection method for lacidipine and fluvoxamine in accident site serum, and relates to the technical field of trace drug detection in biological samples. The method comprises the following steps: preparing a surface enhanced Raman spectrum substrate with gold nanoparticles loaded on amino modified MIL-101 (Cr), and combining sample extraction, oscillation adsorption, centrifugal separation and Raman spectrum detection: mixing a sample solution and the substrate according to a volume ratio of 1: 3, carrying out oscillation adsorption at 35 DEG C for 30-40 minutes, then carrying out centrifugal collection, collecting a spectrum signal by adopting a 785nm laser, and calculating the Raman spectrum based on the spectrum signal. And the qualitative analysis of the target drug is realized by identifying the characteristic peaks of 1632 cm <-1 > + / -2 cm <-1 > and 1060 cm <-1 > + / -2 cm <-1 >. The method has the characteristics of rapidness, high efficiency, no need of complex pretreatment and high sensitivity, is suitable for rapid detection of lacidipine and fluvoxamine in a serum sample on an accident site, and provides technical support for clinical first aid and judicial expertise.

The invention relates to the technical field of terahertz sensing, and discloses a method for detecting trace p-xylene gas based on an HKUST-1 modified terahertz metasurface sensor. The method comprises the following steps: modifying the surface of a sensor with HKUST-1, placing the modified sensor in a gas chamber, testing the terahertz spectrum of the modified sensor in a no-load state, introducing paraxylene gas with different concentrations into the gas chamber, and collecting response spectrums with different concentrations by using a spectrum system; further, comparing the terahertz spectrum in the no-load state with the response spectrum, and establishing a relational expression according to the frequency shift amount of the spectrum; furthermore, according to the operation method, the selectivity of the sensor is verified by comparing response spectrums of the sensor to other types of gas samples with the same concentration. The method for detecting trace p-xylene gas based on the HKUST-1 modified metasurface sensor is simple to operate at room temperature, and has important application value in the field of sensitive and selective p-xylene gas detection.

The application discloses a kind of composite gel breaking-directional adsorption purification combined special medical thickening component nitrate rapid detection method, belong to food detection technical field.The application is aimed at special medical food thickening component nitrate detection, colloid structure causes target extraction efficiency to be low, gel breaking effect and heat-sensitive component protection are difficult to give consideration to, method adaptability is poor, matrix interference is serious technical problem, first by LDA linear discriminant analysis model realizes sample classification and targeted gel breaking scheme matching, then adopts differentiating composite gel breaking system to cooperate entropy weight method-TOPSIS dynamic end point determination, by colloid polysaccharide characteristic fragment surface imprinting mesoporous nanometer silicon dioxide directional adsorption purification, finally by 15 N mark temperature-sensitive type isotopic internal standard is combined with three-wavelength in-situ calibration to complete quantitative detection.The method has good detection accuracy and repeatability, is suitable for multi-scene detection requirements, and can meet the quality control requirements of trace nitrate detection of special medical food.

The invention belongs to the technical field of biological detection, and particularly relates to a method for analyzing and detecting IgG N-glycan in dried blood spots. The dried blood spot sample is adopted to replace a traditional serum sample, so that the technical problem of IgG N-carbohydrate chain degradation under the normal-temperature storage condition of the serum sample is effectively solved, and the stability of the sample and the reliability of a detection result are remarkably improved. The dried blood spot sample collecting method is simple and convenient, vein blood sampling is not needed, the method is suitable for large-scale screening and application of special crowds, and the clinical application range of IgG N-glycan analysis is widened. Besides, the dried blood spot sample can be stored for a long time at room temperature, the transportation and storage cost is reduced, meanwhile, analysis can be completed only through a trace amount of blood, the method is suitable for repeated sampling and long-term monitoring, and a technical support is provided for research and application of IgG glycosylation as a biomarker.

The utility model discloses a device for detecting the concentration of multi-component trace gas, and particularly relates to the technical field of gas detection equipment, the device comprises a gas chromatograph main body, one side of the outer surface wall of the gas chromatograph main body is connected with a gas inlet pipe, the lower end of the gas inlet pipe is fixedly connected with a fixed seat on the outer surface wall of the gas chromatograph main body, and the fixed seat is fixedly connected with the gas chromatograph main body. A treatment cylinder is connected to the interior of the fixed seat, a partition plate is connected to the interior of the treatment cylinder, and a concentration structure is arranged on one side of the partition plate. By arranging the concentration structure, low-concentration trace gas can be concentrated and enriched, extremely-low-concentration gas which is originally difficult to detect is gathered in quantity, the concentration of the trace gas in a gas sample is greatly improved, and a detection signal becomes easy to capture and analyze from weak, so that the detection sensitivity is greatly enhanced; trace gas components which are possibly omitted in the past can be accurately found, and the reliability and the accuracy of a detection result are improved.

The invention discloses a method for efficiently detecting trace residues of'three-high 'drugs in fish plasma, the'three-high' drugs comprise triamterene, terazosin, rosiglitazone, lovastatin, benazepril, pitavastatin and repaglinide, and the method comprises the following steps: adding an anticoagulant into a collected fish plasma sample, centrifuging, taking supernate, and collecting the supernate; the method comprises the following steps: firstly, adding an internal standard solution, adding acetone containing formic acid with the volume fraction of 1% into a purification tube, loading a labeled sample, pressurizing to ensure that the sample drips in a drop shape, collecting by using a polypropylene test tube containing a purification reagent, oscillating and purifying, centrifuging, taking supernate, and concentrating to obtain a concentrated solution; the method disclosed by the invention is high in sensitivity, and the detection limit range of seven kinds of drugs for target'three highs' in the blood plasma of the aquatic environment fish is 0.02-0.25 ng / mL.

The application discloses a liquid inlet device of a thrombolytic catheter, which comprises a catheter main body, a sealed box and a propelling assembly. The outer side of the catheter main body is sleeved with an outer pipe, the inner side surface of the outer pipe is provided with a flow guide groove, the inner bottom wall of the flow guide groove is provided with a plurality of flow guide holes, the outer pipe end part located on the outer side of a human body is fixedly connected with a flow guide ring which is internally hollow, each flow guide groove is communicated with the inner part of the flow guide ring, the sealed box is placed with a bag body which internally contains an anticoagulant liquid, the sealed box is communicated with the inner part of the flow guide ring through a branch pipe, and the propelling assembly is communicated with the inner part of the sealed box through a connecting pipe. The sealed box is inflated through the propelling assembly, the air pressure is enhanced to extrude the bag body when the gas enters the sealed box, the anticoagulant liquid in the bag body is slowly and continuously flowed into a plurality of flow guide grooves through the branch pipe, and then is flowed out of a blood vessel cavity, so that the formation of a cavity intramural thrombus is reduced, and then the whole blood vessel cavity continuously has a trace amount of anticoagulant drug, which can effectively dissolve the thrombus in the blood vessel cavity and on the outer wall of the thrombolytic catheter.

The invention relates to the technical field of biological medicine detection, and particularly discloses an enrichment detection method for trace drug metabolites in a clinical biological sample. In order to solve the problems that samples are complex, trace substances are difficult to detect and the like, through a nano material-aptamer collaborative enrichment module, a micro-fluidic chip-ultrasonic extraction module, a multi-dimensional chromatography-high-resolution mass spectrometry module and the like, a quantitative algorithm fused by a background noise suppression algorithm based on deep learning and a dynamic programming-genetic algorithm is combined; the efficient enrichment, accurate detection and accurate analysis of the metabolite are realized. Meanwhile, the intelligent quality control module ensures that the result is reliable. According to the method, the detection sensitivity and accuracy are remarkably improved, and powerful support is provided for clinical drug monitoring and disease diagnosis.

The invention discloses a method for detecting N-nitroso ambroxol in a medicine through LC-MS / MS, and belongs to the technical field of medicine analysis. According to the method for detecting N-nitroso ambroxol in the medicine through LC-MS / MS, the medicine is an ambroxol-containing preparation or ambroxol and a salt bulk drug thereof, and liquid chromatography conditions comprise that octadecylsilane chemically bonded silica is used as a filler, 0.05-0.2% formic acid is used as a mobile phase A, and acetonitrile is used as a mobile phase B for gradient elution; the mass spectrum conditions comprise that a mass spectrum detector and an electrospray positive ion mode are adopted, multi-reaction detection is carried out, and m / z 377.9 to 279.2, m / z 377.9 to 114.3 and m / z 377.9 to 96.3 are selected as detection ion pairs. The method for detecting the ambroxol-containing preparation and the raw materials by adopting LC-MS / MS is high in sensitivity, good in accuracy and suitable for quality control of trace nitrosamine impurities in the preparation and the raw materials.

The invention provides a method for detecting the residual quantity of micro or trace fosfomycin tromethamine, and belongs to the field of pharmaceutical analysis. According to the method, a low-capacity hydroxyl system cathode protection column and a high-capacity anion exchange separation column are used as chromatographic columns, an anion electrolysis regeneration suppressor is adopted, a sodium hydroxide or potassium hydroxide solution with the current of 36 mA to 44 mA and the current of 15 mmol / L to 17 mmol / L is used as leacheate, and the residual quantity of fosfomycin tromethamine is calculated according to the peak area by an external standard method. Compared with the prior art, the method has the advantages that the sensitivity is high, the quantitation limit of fosfomycin trometamol is as low as 0.0475 mu g / ml, the reproducibility is good, the operation is simple, the detection condition is mild, the application of detection equipment and instruments is wide, the source of leacheate is wide, the preparation is simple, and the like.

The present invention relates to the field of analytical chemistry, and particularly to a solid-phase microextraction probe based on metal-organic framework SU101, its preparation method, and its application in the trace detection of pharmaceuticals and personal care products in water environment. The solid-phase microextraction probe provided by the present invention has environmentally friendly materials and significantly stronger extraction ability than commercial probes, and has the advantages of low detection limit and quantification limit, wide linear range, good reproducibility, etc. Therefore, it can be used to detect extremely trace amounts of polar drugs in environmental water, especially glucocorticoid drugs.

To enable high-sensitivity detection of peach that might cause allergies contained in food ingredients and products even if the amount is minute.SOLUTION: A method of detecting peach is provided, comprising extracting protein from a sample, obtaining an enzyme digest of the extracted protein using a protease, and analyzing the enzyme digest to detect at least one peptide selected from a group consisting of SEQ ID NOs: 1 through 3 using a mass spectrometer so as to qualitatively or quantitatively determine the presence or absence of peach protein in the sample.SELECTED DRAWING: Figure 1

The invention relates to a trace hydroxyputrescine lysine protein detection method, which comprises: 1) preparing a sample to be detected, and slicing cells, body fluid or tissue; (2) sealing the sample by using Duolink sealing liquid; (3) diluting an anti-rabbit PLUS probe and an anti-mouse MINUS probe Merck and DUO92101 / DUO92102 in proportion by using a Duolinkantibody diluent, incubating with a product obtained in the previous step, and respectively combining a rabbit polyclonal antibody and a mouse monoclonal antibody through the PLUS / MINUS probe to provide a basis for a ligation reaction; (4) diluting ligase by using a Duolink ligation buffer solution according to the ratio, and incubating with the product in the step (3) at the temperature; 5) diluting an amplification enzyme with a Duolink amplification buffer solution in proportion, and incubating with a product obtained in the previous step; 6, a mounting medium containing DAPI is adopted for mounting, and imaging analysis is carried out through a fluorescence or confocal microscopy. The problem that due to the fact that the affinity of the antibody is insufficient and the protein expression quantity is low, the omission ratio of the trace hydroxyputrescine lysine modified protein is high can be well solved.

The invention belongs to the technical field of atomic absorption spectrometry analysis, and particularly relates to a method for rapidly determining trace Pb in a complex sample under high acidity based on atomic absorption spectrometry, in particular to an analysis method for accurately and rapidly determining trace Pb in cosmetics. According to the method for rapidly determining the trace Pb in the complex sample under the high acidity based on the atomic absorption spectrometry, the HCl concentration of a test system can be greatly improved to 10% by adopting a designed chemical atomizer and optimizing the conditions of reduction reaction; the method breaks through the bottleneck that trace Pb in a few simple samples can only be measured under low acidity by a hydride generation method, so that the chemical reaction of Pb and NaBH4 can be stably carried out under high acidity, the problem of testing trace Pb in complex samples is solved, and the method is high in sensitivity, simple and rapid to operate and reliable in test result.