96 results about "Trace Amounts" patented technology

This invention discloses a rapid mass spectrometry method and kit for the simultaneous detection of multiple components in body fluids, belonging to the field of biological detection and identification technology. It can rapidly detect trace compounds such as drugs and biomarkers in complex biological samples. This kit mainly utilizes loaded functional materials to achieve extraction and enrichment of target analytes and is equipped with a quick connector for coupling with a detector to achieve rapid detection. Compared with traditional methods, the significant advantages of the method established by this kit are that it does not require complex sample pretreatment, allows for micro-quantitative sampling, requires small sample volumes, facilitates convenient storage of biological samples, is easy to operate, does not require specialized technical personnel, and enables rapid on-site detection when coupled with portable mass spectrometry. This kit has been successfully used for the detection of quinine, procainamide hydrochloride, atenolol, and nortriptyline in complex samples such as blood and serum, and has significant clinical and economic value.

The utility model provides a disposable needleless injection needle which comprises a pressure source instrument connecting part, a liquid medicine injection part, a liquid medicine storage part and a liquid medicine injection part, and a screw sequentially penetrates through a screw hole of the liquid medicine injection part and a screw hole of the liquid medicine storage part and finally enters a threaded hole of the pressure source instrument connecting part to achieve overall fixation. The side wall of the liquid medicine injection part and the side wall of the liquid medicine storage part are fixed through screws, the pressure source instrument connecting part is connected with an external micro-jet injector to obtain a pressure source, and the liquid medicine injection part is connected with an external injection tube to obtain liquid medicine. And the continuous broken injection of the liquid medicine is realized. The utility model has the beneficial effects that a trace amount of medicine can be quickly and accurately injected into the skin surface layer, so that pain and recovery time are reduced; under the condition that the needle head is not replaced, liquid medicine can be added at any time, and convenience and rapidness are achieved.

The invention discloses a double-layer detachable purification and enrichment device and a use method thereof, and relates to the technical field of sample analysis. The device comprises an enrichment column, a purification column and a needle head, the enriching column is detachably connected with the purifying column; as required, the needle head is detachably connected with the enriching column or the purifying column; wherein the enrichment column is in an injector shape, and a piston, a first filter disc, an enrichment material and a second filter disc are sequentially arranged in a cavity in the enrichment column from top to bottom; the piston moves up and down in the cavity, the first filter disc, the enrichment material and the second filter disc are arranged at the bottom in the cavity, and the enrichment material is filled in an interlayer between the first filter disc and the second filter disc and is fixed; a third filter disc, a purification material and a fourth filter disc are arranged in the purification column; and the purification material is filled in an interlayer between the third filter disc and the fourth filter disc and is fixed. The device integrates the processes of sampling, purification, enrichment, elution, sample loading and the like, and can realize rapid purification, enrichment and elution of trace target objects in a solution sample.

The utility model discloses a portable traditional Chinese medicine olfactory diagnosis instrument, which comprises a sample collection device, a gas enrichment device, a mass spectrometer, a data processor and a display, the mass spectrometer is sequentially connected with the data processor and the display, the gas enrichment device is test paper with an enrichment reagent encapsulating nano-metal particles, the enrichment efficiency is effectively improved, and the detection accuracy is improved. Therefore, the detection sensitivity and accuracy of the mass spectrometer are improved; the device is provided with the ion trap mass spectrometer, has the characteristics of high detection speed, high resolution and high sensitivity, and has performance advantages in qualitative and trace analysis aspects; and due to small volume and strong portability, the device can be conveniently integrated into portable or field detection equipment, can be used for auxiliary diagnosis of heart failure and asthma, and also has great clinical application value in the aspects of disease evolution and disease prognosis evaluation.

The utility model relates to an adsorption device for simply measuring gaseous iodine, which comprises a large glass bottle and a small glass bottle, the upper ends of the large glass bottle and the small glass bottle are open, the large glass bottle serves as a container for bearing solid iodine, iodine adsorption materials are placed in the small glass bottle, the small glass bottle is placed in the large glass bottle through a support structure arranged in the large glass bottle, and the upper surface of the support structure is a plane. The support structure separates the small glass bottle from the large glass bottle, and the top of the small glass bottle is not in contact with the bottom of the large glass bottle cap. According to the utility model, through the measures, experiments or operations which may have risks when contact time between experimenters and instruments is reduced are realized, and the safety of operators is ensured; the adsorption effect of the measurement material is enhanced, the test time is shortened, the accuracy, precision and sensitivity of weighing analysis are improved, and the method is greatly helpful for analysis of trace samples and trace components.

The present invention relates to an equal-dose opposed-vessel monitoring pulmonary artery-pulmonary vein CT angiography method and system. For the method, the dose of the contrast agent bolus test, the flow rate and volume of the contrast agent, and the flow rate and volume of the normal saline are all consistent with those in the formal scan; during the bolus test, it is confirmed that a small amount of contrast agent flows into the pulmonary vein of the monitored slice while the pulmonary artery is filled, so as to obtain the optimal delay time of pulmonary artery CTA; it is confirmed that almost all of the contrast agent in the monitored slice has flowed through the pulmonary artery, and there is a trace amount of contrast agent at the end of the pulmonary artery near the periphery, so as to obtain the optimal delay time of pulmonary vein CTV. The system includes each module for realizing the above functions. The present invention can achieve accurate timing of pulmonary artery CTA and pulmonary vein left atrial CTV scans, and can also achieve accurate timing of combined one-stop right atrium ventricle pulmonary artery and pulmonary vein left atrium ventricle combined scans, realizing precise diagnosis and treatment.

The invention discloses a magnetic multi-walled carbon nanotube material for enriching and synthesizing cannabinoid drugs as well as a preparation method and application of the magnetic multi-walled carbon nanotube material, the magnetic multi-walled carbon nanotube material is of a core-shell structure, Fe3O4 wrapping carboxylated multi-walled carbon nanotubes is taken as a central core, and the central core is externally coated with a load layer composed of dopamine and chitosan; the magnetic multi-walled carbon nanotube material provides a novel functional platform for solid-phase extraction detection of trace synthetic cannabinoid drugs due to a multi-action site synergistic mechanism and a simple magnetic separation characteristic, and is particularly suitable for high-throughput sample pretreatment in sewage toxicity monitoring; the method is simple and convenient in the process of treating trace synthetic cannabinoid drugs in a complex matrix sample, greatly improves the detection sensitivity of an instrument, can be matched with an on-site rapid detection instrument or a laboratory large-scale analysis instrument for use, reduces pretreatment steps, avoids the defects of complex steps and low repeatability in the prior art, and has a great application prospect.

This invention discloses a method for enhancing the terahertz absorption spectrum of materials. The method uses a terahertz time-domain spectroscopy system to measure the material spectrum, calculates the material's transfer function, amplitude, and phase using the acquired reference signal and sample signal, fits the material's absorption spectrum by constructing a multi-Gaussian function model, and enhances the absorption spectrum through a coefficient enhancement factor. This strengthens the ability to identify minute differences in the terahertz spectrum and improves the ability of terahertz spectroscopy to distinguish substances with similar properties, similar compositions, and trace amounts.

The application discloses a raw material medicine trace heavy metal ion detection method and system based on a micro-fluidic chip, relates to the technical field of micro-fluidic detection and electrochemical analysis, and comprises the following steps: weighing, constant volume, clarification of the raw material medicine sample, solidifying target ions, standard calibration and peak interval / scanning parameters into a detection parameter table; according to the detection parameter table, the flow and valve control are controlled to complete liquid preparation, muSPE enrichment, washing, elution and constant volume loading on the chip; blank, no standard addition, one-time standard addition and numbered archiving are sequentially measured; baseline is made according to the solidified parameters, integration and blank deduction are carried out, the solution concentration is calculated combined with standard addition measurement, and the content is output. The method disclosed by the application can constrain micro-fluidic liquid preparation, enrichment, elution and constant volume loading, so that blank, no standard addition and standard addition three rounds are measured under the same source condition; the curve and standard addition measurement are numbered and archived, integral, blank deduction and concentration are solved simultaneously, and the quantitative consistency, traceability and calculation reliability are improved.

The invention discloses a quantitative method for detecting trace free chelate deferoxamine in ACCs medicine based on reversed phase chromatography. The quantitative method comprises the following steps: preparing a deferoxamine standard substance solution; the method comprises the following steps: respectively detecting a sample to be detected and a deferoxamine standard substance solution by adopting a liquid chromatograph under the detection conditions that a chromatographic column is PLRP-S (2.1 * 100 mm, 5 [mu] m), the column temperature is 25-80 DEG C, and a filler is PS / DVB porous particles; mobile phases: a phase A is an aqueous solution containing organic acid, and a phase B is an organic solvent containing organic acid; establishing a calibration curve according to the concentration of the standard deferoxamine solution and the peak area of the corresponding elution peak, and then substituting the peak area of the elution peak of the sample to be detected into the calibration curve to obtain the concentration of the deferoxamine in the sample to be detected. According to the method, the tedious sample pretreatment step is omitted, the direct sample injection analysis of the sample is realized, the experimental period is greatly shortened, and the detection efficiency is improved.

To address the difficulties of collecting sebum gas from the human body and its low analysis accuracy, the present invention discloses a device for collecting sebum gas and enhancing its infrared spectrum. By combining a micro-electromechanical system (MEMS), functional two-dimensional materials, and metal surface plasmon resonance (MSPR) technology, the present invention produces a device similar in shape and size to a conventional Band-Aid. By applying the device to the human body for 3-5 minutes, trace amounts of sebum gas can be collected. Furthermore, the device can enhance the weak infrared spectrum of sebum gas, further improving the recognition of the sebum gas infrared spectrum, providing a simple, efficient, and accurate device for diagnosing diseases based on sebum gas.

The present invention focuses on a surface-enhanced Raman nanoparticle and its application in detecting fentanyls, within the realm of drug safety assessment. The surface-enhanced Raman nanoparticle comprises a noble metal, with gold nanocakes being the preferred choice. The detection method for fentanyls employs the surface-enhanced Raman nanoparticle described in the present invention to conduct Raman detection. This nanoparticle and the associated detection method enable the rapid and non-destructive identification of trace amounts of fentanyls in the target sample, presenting significant potential for application in public safety detection.

The application discloses a kind of composite gel breaking-directional adsorption purification combined special medical thickening component nitrate rapid detection method, belong to food detection technical field.The application is aimed at special medical food thickening component nitrate detection, colloid structure causes target extraction efficiency to be low, gel breaking effect and heat-sensitive component protection are difficult to give consideration to, method adaptability is poor, matrix interference is serious technical problem, first by LDA linear discriminant analysis model realizes sample classification and targeted gel breaking scheme matching, then adopts differentiating composite gel breaking system to cooperate entropy weight method-TOPSIS dynamic end point determination, by colloid polysaccharide characteristic fragment surface imprinting mesoporous nanometer silicon dioxide directional adsorption purification, finally by 15 N mark temperature-sensitive type isotopic internal standard is combined with three-wavelength in-situ calibration to complete quantitative detection.The method has good detection accuracy and repeatability, is suitable for multi-scene detection requirements, and can meet the quality control requirements of trace nitrate detection of special medical food.

The invention belongs to the technical field of biological detection, and particularly relates to a method for analyzing and detecting IgG N-glycan in dried blood spots. The dried blood spot sample is adopted to replace a traditional serum sample, so that the technical problem of IgG N-carbohydrate chain degradation under the normal-temperature storage condition of the serum sample is effectively solved, and the stability of the sample and the reliability of a detection result are remarkably improved. The dried blood spot sample collecting method is simple and convenient, vein blood sampling is not needed, the method is suitable for large-scale screening and application of special crowds, and the clinical application range of IgG N-glycan analysis is widened. Besides, the dried blood spot sample can be stored for a long time at room temperature, the transportation and storage cost is reduced, meanwhile, analysis can be completed only through a trace amount of blood, the method is suitable for repeated sampling and long-term monitoring, and a technical support is provided for research and application of IgG glycosylation as a biomarker.

The utility model discloses a device for detecting the concentration of multi-component trace gas, and particularly relates to the technical field of gas detection equipment, the device comprises a gas chromatograph main body, one side of the outer surface wall of the gas chromatograph main body is connected with a gas inlet pipe, the lower end of the gas inlet pipe is fixedly connected with a fixed seat on the outer surface wall of the gas chromatograph main body, and the fixed seat is fixedly connected with the gas chromatograph main body. A treatment cylinder is connected to the interior of the fixed seat, a partition plate is connected to the interior of the treatment cylinder, and a concentration structure is arranged on one side of the partition plate. By arranging the concentration structure, low-concentration trace gas can be concentrated and enriched, extremely-low-concentration gas which is originally difficult to detect is gathered in quantity, the concentration of the trace gas in a gas sample is greatly improved, and a detection signal becomes easy to capture and analyze from weak, so that the detection sensitivity is greatly enhanced; trace gas components which are possibly omitted in the past can be accurately found, and the reliability and the accuracy of a detection result are improved.

The invention discloses a method for efficiently detecting trace residues of'three-high 'drugs in fish plasma, the'three-high' drugs comprise triamterene, terazosin, rosiglitazone, lovastatin, benazepril, pitavastatin and repaglinide, and the method comprises the following steps: adding an anticoagulant into a collected fish plasma sample, centrifuging, taking supernate, and collecting the supernate; the method comprises the following steps: firstly, adding an internal standard solution, adding acetone containing formic acid with the volume fraction of 1% into a purification tube, loading a labeled sample, pressurizing to ensure that the sample drips in a drop shape, collecting by using a polypropylene test tube containing a purification reagent, oscillating and purifying, centrifuging, taking supernate, and concentrating to obtain a concentrated solution; the method disclosed by the invention is high in sensitivity, and the detection limit range of seven kinds of drugs for target'three highs' in the blood plasma of the aquatic environment fish is 0.02-0.25 ng / mL.

The application discloses a liquid inlet device of a thrombolytic catheter, which comprises a catheter main body, a sealed box and a propelling assembly. The outer side of the catheter main body is sleeved with an outer pipe, the inner side surface of the outer pipe is provided with a flow guide groove, the inner bottom wall of the flow guide groove is provided with a plurality of flow guide holes, the outer pipe end part located on the outer side of a human body is fixedly connected with a flow guide ring which is internally hollow, each flow guide groove is communicated with the inner part of the flow guide ring, the sealed box is placed with a bag body which internally contains an anticoagulant liquid, the sealed box is communicated with the inner part of the flow guide ring through a branch pipe, and the propelling assembly is communicated with the inner part of the sealed box through a connecting pipe. The sealed box is inflated through the propelling assembly, the air pressure is enhanced to extrude the bag body when the gas enters the sealed box, the anticoagulant liquid in the bag body is slowly and continuously flowed into a plurality of flow guide grooves through the branch pipe, and then is flowed out of a blood vessel cavity, so that the formation of a cavity intramural thrombus is reduced, and then the whole blood vessel cavity continuously has a trace amount of anticoagulant drug, which can effectively dissolve the thrombus in the blood vessel cavity and on the outer wall of the thrombolytic catheter.

The invention relates to the technical field of biological medicine detection, and particularly discloses an enrichment detection method for trace drug metabolites in a clinical biological sample. In order to solve the problems that samples are complex, trace substances are difficult to detect and the like, through a nano material-aptamer collaborative enrichment module, a micro-fluidic chip-ultrasonic extraction module, a multi-dimensional chromatography-high-resolution mass spectrometry module and the like, a quantitative algorithm fused by a background noise suppression algorithm based on deep learning and a dynamic programming-genetic algorithm is combined; the efficient enrichment, accurate detection and accurate analysis of the metabolite are realized. Meanwhile, the intelligent quality control module ensures that the result is reliable. According to the method, the detection sensitivity and accuracy are remarkably improved, and powerful support is provided for clinical drug monitoring and disease diagnosis.

The present invention relates to the field of analytical chemistry, and particularly to a solid-phase microextraction probe based on metal-organic framework SU101, its preparation method, and its application in the trace detection of pharmaceuticals and personal care products in water environment. The solid-phase microextraction probe provided by the present invention has environmentally friendly materials and significantly stronger extraction ability than commercial probes, and has the advantages of low detection limit and quantification limit, wide linear range, good reproducibility, etc. Therefore, it can be used to detect extremely trace amounts of polar drugs in environmental water, especially glucocorticoid drugs.

To enable high-sensitivity detection of peach that might cause allergies contained in food ingredients and products even if the amount is minute.SOLUTION: A method of detecting peach is provided, comprising extracting protein from a sample, obtaining an enzyme digest of the extracted protein using a protease, and analyzing the enzyme digest to detect at least one peptide selected from a group consisting of SEQ ID NOs: 1 through 3 using a mass spectrometer so as to qualitatively or quantitatively determine the presence or absence of peach protein in the sample.SELECTED DRAWING: Figure 1

The invention relates to a trace hydroxyputrescine lysine protein detection method, which comprises: 1) preparing a sample to be detected, and slicing cells, body fluid or tissue; (2) sealing the sample by using Duolink sealing liquid; (3) diluting an anti-rabbit PLUS probe and an anti-mouse MINUS probe Merck and DUO92101 / DUO92102 in proportion by using a Duolinkantibody diluent, incubating with a product obtained in the previous step, and respectively combining a rabbit polyclonal antibody and a mouse monoclonal antibody through the PLUS / MINUS probe to provide a basis for a ligation reaction; (4) diluting ligase by using a Duolink ligation buffer solution according to the ratio, and incubating with the product in the step (3) at the temperature; 5) diluting an amplification enzyme with a Duolink amplification buffer solution in proportion, and incubating with a product obtained in the previous step; 6, a mounting medium containing DAPI is adopted for mounting, and imaging analysis is carried out through a fluorescence or confocal microscopy. The problem that due to the fact that the affinity of the antibody is insufficient and the protein expression quantity is low, the omission ratio of the trace hydroxyputrescine lysine modified protein is high can be well solved.

The invention belongs to the technical field of atomic absorption spectrometry analysis, and particularly relates to a method for rapidly determining trace Pb in a complex sample under high acidity based on atomic absorption spectrometry, in particular to an analysis method for accurately and rapidly determining trace Pb in cosmetics. According to the method for rapidly determining the trace Pb in the complex sample under the high acidity based on the atomic absorption spectrometry, the HCl concentration of a test system can be greatly improved to 10% by adopting a designed chemical atomizer and optimizing the conditions of reduction reaction; the method breaks through the bottleneck that trace Pb in a few simple samples can only be measured under low acidity by a hydride generation method, so that the chemical reaction of Pb and NaBH4 can be stably carried out under high acidity, the problem of testing trace Pb in complex samples is solved, and the method is high in sensitivity, simple and rapid to operate and reliable in test result.

The utility model discloses a centrifugal machine and a workstation for extracting and enriching trace microorganisms from a complex matrix, the centrifugal machine comprises a main body frame, a partition plate is arranged in the middle of the main body frame, and the centrifugal machine is divided into a centrifugal chamber and an accessory chamber by the partition plate; the rotor assembly is arranged above the partition plate, is positioned in the centrifugal chamber and is used for placing a sample; a refrigeration assembly is arranged on the outer wall of the centrifugal chamber and used for cooling or refrigerating the centrifugal chamber; the positioning assembly is arranged below the partition plate and located in the accessory chamber. When the centrifugal machine is used for separating and enriching infectious samples, most of impurities such as human cells, proteins, saccharides, salts and the like in uncultured or cultured clinical samples such as blood, cerebrospinal fluid, urine, hydrothorax and ascites and the like can be effectively removed, and relatively pure living pathogens are obtained.

[Problem] To detect abalone, which may cause an allergic reaction, with high sensitivity even when trace amounts of abalone is contained in a food raw material, a product or the like. [Solution] Provided is a method for detecting abalone, the method comprising a step for extracting a protein from a sample, and a step for allowing the extracted protein to react with a protease to produce an enzymatically digested product, and the method involving analyzing the enzymatically digested product, and detecting at least one peptide selected from the group consisting of SEQ ID NO:1 to SEQ ID NO:2 by using a mass spectrometer to qualitatively or quantitatively determine whether or not an abalone protein is present in the sample.

1. Name of the Design Product: Pipette. 2. Use of the Design Product: Used for transferring small or trace amounts of liquid in the laboratory. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.

The present invention belongs to the technical field of medical device recycling, and discloses a medical device part recycling and processing device, which includes a housing. A baffle is fixedly connected to the bottom of the housing. Collection chambers are arranged on the front and rear sides of the baffle. Above the rear collection chamber, there is a gas distribution housing fixedly connected to the housing. A connecting pipe is communicated with the front end of the gas distribution housing, and a heater is communicated with the front end of the connecting pipe. By rotating the upper push rod and the lower push rod, the upper and lower sliding plates are staggered to move forward and backward. While ensuring the feeding of medical device parts, the volatilized toxic substances, generated dust and particles cannot be discharged from the feeding port into the atmosphere, thus ensuring the personal safety of the staff. Moreover, when the lower sliding plate opens for feeding, the trace amount of gas that may be discharged is suctioned back into the interior of the housing again, further avoiding the diffusion of toxic substances, generated dust and particles into the air, and solving the problem of serious environmental pollution caused by the prior art.

The utility model discloses a dose-adjustable pressing sprayer which comprises a screwing cap used for being connected with an external container, a pressure pump body is installed in the screwing cap, a pump pipe is arranged at the top of the pressure pump body, one end of the pump pipe penetrates through the screwing cap and is externally connected with a pressing pump head, and the other end of the pump pipe penetrates through the pressing pump head. The bottom of the pressure pump body is connected with a liquid suction pipe, and the pressure pump is characterized in that the screwing cap is provided with a table top used for installing an elastic telescopic piece. According to the pressing spray head with the adjustable dosage, the quantitative parts with different heights on the trapezoidal quantitative unit are matched with the positioning head on the elastic telescopic piece, so that a user can select different pressing gears by rotating the pressing pump head. And each quantitative part corresponds to a specific pressing stroke, so that the single-time liquid outlet amount is accurately controlled. By means of the design, the dosage requirement from trace use (such as perfume) to large use (such as shower gel) can be met, and waste or complexity of repeated pressing is effectively avoided.

The invention belongs to the technical field of atomic spectrum analysis and detection, and particularly relates to a method for separating trace Se and As in a multi-metal sample and a method for rapidly detecting the trace Se and As in the multi-metal sample based on atomic absorption spectrometry. According to the method for separating trace Se and As in the multi-metal sample, a miniature cation chromatographic column is adopted, especially a zero empty bed volume leaching technology is adopted, the column flow rate can be as high as several milliliters per minute, the elution volume is only 1-2 milliliters, the separation effect is close to 100%, only 2-3 minutes are needed for collecting As or Se flowing through a column bed, the separation speed is high, and the separation efficiency is high. And the determination result is accurate and reliable.

The present invention discloses a method and system for detecting trace substances based on terahertz hyperspectral imaging, which relates to the field of trace substance detection, including: improving the efficiency of the interaction between the light field and the sample to be tested through the localized light field of the metasurface; collecting hyperspectral image data of the sample through terahertz hyperspectral imaging technology; extracting characteristic information from the spectral data using a feature extraction algorithm; and conducting quantitative analysis by establishing a model based on the characteristic information, and obtaining information such as the concentration and composition of the trace substance. Detection using terahertz hyperspectral imaging technology has the characteristics of high sensitivity and non-destructive testing, and can perform rapid, accurate, and quantitative detection of trace substances. In addition, this method does not require sample processing, has low requirements for the sample preparation process on the sensor surface, and avoids sample contamination, detection errors, and errors caused by uneven distribution of trace samples on the structural surface.

The invention discloses a method for determining trace elements in oral medicines by ICP-MS, comprising the following steps: grinding, digestion, secondary digestion, sampling and constant volume, and sample introduction analysis. A combined digestion solution of hydrofluoric acid and nitric acid is used in the pre-treatment process of the present invention, so that digestion can be complete, the blank value of the reagent is reduced, and the influence of interfering impurities on the elements to be measured is reduced. Microwave digestion is adopted after adding nitric acid simultaneously, and a temperature program is performed to improve the digestion effect, reduce the influence of the digestion reaction on the determination of element content, and after adding hydrofluoric acid, graphite digestion instrument is used for digestion, and 120 DEG C of digestion is performed for 60min, shortening the digestion time, improving digestion efficiency, and simultaneously using ICP-MS determination, the content of multiple elements in oral medicine tablets can be determined simultaneously, with low detection limit and high sensitivity, and is particularly suitable for the analysis of trace elements.