1428results about How to "Easy to breed" patented technology

The presently claimed invention provides methods and kits for amplifying a target sequence from within a nucleic acid population. The presently claimed invention provides selection probes which are complementary to at least a portion of said target sequence and mechanisms for adding a probe sequence to the 3' end of a target sequence that is hybridized to a selection probe. The added 3' probe sequence and a probe sequence added at the 5' end of the target by adaptor ligation allow for selective amplification of the target sequence.

The invention discloses a method for site-specific mutagenesis of medicago sativa genes by employing a CRISPR/Cas9 system. First of all, a binary expression vector MsCRISPR/Cas9 is constructed; the vector is designed and constructed for a target gene; then, agrobacterium tumefaciens-mediated transformation is used to achieve site-specific mutagenesis of a specific gene, and a mutant transformed plant with the target gene knocked out is obtained by screening; the method uses the CRISPR/Cas9 technology for cross-pollination autotetraploid plant medicago sativa for the first time, and obtains a mutant plant; a MtU6 promoter is used for the first time to initiate sgRNA transcription in the medicago sativa, thereby improving the transcription efficiency; the mutation rate can reach 52%; the method realizes direct mutation on the coding sequence of the gene, and opens up a new field for gene editing in the medicago sativa, laying a technical foundation for simultaneous silencing of a plurality of genes, deletion of large chromosome fragments, precise insertion of foreign genes into targets, gene regulation and the like.

An apparatus to transfer gas to or from a liquid has a flexible and oxygen permeable but liquid water impermeable membrane, a flexible and gas permeable spacer, an inlet conduit, an outlet conduit and a non-rigid restrain system. When used for treating wastewater, an aerobic biofilm is culture adjacent the planar elements, an anoxic biofilm is cultivated adjacent the aerobic biofilm and the wastewater is maintained in an anaerobic state. A first reactor for treating wastewater has an anaerobic section, a plurality of gas transfer membrane modules, and an aerobic section. A biofilm is cultivated on the surface of the gas transfer membranes in fluid communication with the anaerobic section. Biological reduction of COD, BOD, nitrogen and phosphorous are achieved. In a second reactor, phosphorous is also removed chemically in a precipitation branch.

The invention provides a method for cultivating earthworms. The method comprises the steps that first, a base box body is sequentially filled with river stones, gravel and silver sand, an earthworm cultivation box is placed on the base box body, organic waste mixtures are arranged in the earthworm cultivation box, then earthworm seedlings are put into the earthworm cultivation box, after the earthworms are cultivated for 30 days, organic waste mixtures are continuously added into the earthworm cultivation box until the earthworm cultivation box is filled with the organic waste mixtures; earthworm cultivation boxes are continuously placed on the earthworm cultivation box filled with the organic waste mixtures, after the earthworm cultivation box at the top layer is filled with the organic waste mixtures, the earthworm cultivation box at the bottom layer is drawn out, and the earthworm cultivation box is placed back to the top layer after earthworm cast in the earthworm cultivation box is cleaned away to achieve circulation cultivation. The method for cultivating the earthworms has the advantages that the facts that earthworms are bred from generation to generation, are inbred and are in variety degeneration are avoided, and breed conservation and reproduction of the earthworms can be better achieved. Adult earthworms are harvested conveniently, the living and incubation environment of the earthworms is not damaged, the incubation rate is guaranteed, and the method is suitable for earthworm industrial large-scale cultivation.

The invention discloses a bee attractant and a method for field seed production and pollination of soybean hybrids. The method comprises the following steps of: selecting a field which is suitable for the growth of soybeans, duly sowing the soybean hybrids, and intercropping male parents and female parents across ridges; selecting a required amount of healthy pollination swarm within 5 to 7 days before pollination, and transporting into the field of the soybeans at the early flowering stage of the soybeans; feeding attractant syrup within 1 hour before the bee attractant is sprayed everyday or every other day in the seed production field of the soybean hybrids which just blossom; spraying the bee attractant on leaves of hybrid soybean plants of the male parents and the female parents in 1 to 5 ridges of field across 1 to 5 ridges of field every 1 to 3 days so as to ensure that bees are attracted into the field and fall down to the leaves of the hybrid soybean plants to eat the syrup; spraying the attractant on two sides of stalks of the hybrid soybean plants to attract the bees to fall down to hybrid soybeans to collect so as to pollinate the hybrid soybeans; and stopping spraying the bee attractant in rainy days, and restoring the spray when the weather gets better so as to maintain the attractant which can meet the requirements in the field. The attractant is obtained by mixing a honeycomb liquid, a flower aroma imitation liquid and syrup in a proportion and diluting by using quantitative cold boiled water.

The invention provides a microalgae grease extraction method which comprises the following steps: regulating the pH value of a microalgae solution, heating, pressurizing, and then returning to the normal pressure state; and adding biological enzymes, and mixing, wherein the biological enzymes are added in two steps, non-proteinase is firstly added, and proteinase is secondly added. According to the method provided by the invention, when the biological enzymes are used for treating wet algae, the proteinase is added after the non-proteinase acts for some time, so that the non-proteinase can not be decomposed or destroyed by the proteinase, thereby greatly increasing the wall-breaking efficiency. Compared with the method of simultaneously adding the proteinase and the non-proteinase, the wall-breaking efficiency is obviously increased, thereby obviously increasing the grease extraction rate and yield.

The invention discloses a method for culturing teak seedlings by spike culture and rapid propagation in containers. High-quality single teak seeds are used as the parent plants; when growing to 4 to 5 centimeters high, the seedlings sown on a sand bed are transplanted into a parent plant containing cup filled with nutrient substrate, and placed on a lifting seedling culturing bed to be cultured; when the diameter of each parent plant is larger than 0.3 centimeter, the parent plant is cut off from the part 10 to 15 centimeters far from the basic part to promote side branches to germinate; when the seedlings are semi-lignified, side branches are cut off, used as cuttings, inserted into a cutting containing cup filled with substrate and placed on the lifting seedling culturing bed to be cultured; therefore, a large amount of high-quality teak seedlings maintaining the excellent hereditary characters of the parent plants can be propagated rapidly in a short time, and the success rate of cottage reaches 85.1 to 96.6 percent. The method for culturing teak seedlings by spike culture and rapid propagation in the containers is not limited by season or region, and can be used for obtaining excellent propagants in a poor land condition; the moisture and nutrition of the parent plants are easier to control; the method is beneficial to culture of desired cutting spikes; the coefficient of propagation is high; the method is simple; the operation is easy; the production cost is low; and the method for culturing teak seedlings can meet the needs in modern forestry development of China.

A method assembling straw biochar with microbe bacteria fertilizer so as to improve saline land relates to the saline land improvement method, and aims to solve the technical problems that an existing saline land cannot plant crops or the crops output is low; the method comprises the following steps: composting and fermenting an organic fertilizer so as to obtain decomposed organic fertilizer, uniformly mixing the straw biochar, the microbe bacteria fertilizer and the decomposed organic fertilizer according to weight ratio: 1-12:1:10-20, and piling up the mixture for preparation; uniformly throwing the straw biochar, the microbe bacteria fertilizer and the decomposed organic fertilizer mixing improvement material on the soil surface 0-2 days before soil working, and appilication amount is 1350kg/mu; doing intersect rotary tillage for 1-2 times after fertilizer application, rotary tillage depth is 12-15cm, immediately suppressing the soil after rotary tillage, and then sowing is carried out. The method is simple, modifiers are straw biochar and manure, the materials are easy to get, so cost is low; the improved saline land is obvious in effect; the method is suitable for saline land improvement.

The invention relates to a culture medium for breeding earthworms and a stereoscopic earthworm breeding method and device and belongs to the technical field of earthworm breeding. The culture medium is prepared through the following method that firstly, a base material and EM bacterium liquid are mixed, humidity is adjusted to 80%-90% to obtain a mixed material, and the base material is mainly composed of livestock feces, coarse fiber materials and sludge; secondly, the mixed material is subjected to closed anaerobic fermentation for 22-26 h, and after temperature is lowered to 28-32 DEG C, the EM bacterium liquid is added to continue the closed anaerobic fermentation for 22-26 h, and a fermented material is obtained; and thirdly, net bags are filled with the fermented material, and after compacting, the culture medium is obtained after piling and thorough decomposing are performed for 1-3 days. The culture medium achieves that pollution is treated through cultivation and achieves effective recycling of organic solid waste, nutrient substances in the base material are reserved to the maximum extent, and the culture medium is suitable for growing and breeding of the earthworms; and a three-dimensional stereoscopic space is provided for activity and breeding of the earthworms, adult earthworms are not needed to be separated manually, and intensified stereoscopic breeding of the earthworms in a greenhouse or in an indoor environment can be achieved easily.

The invention discloses a sweet potato cultivation method in hills and mountains, comprising the following steps of: A, selecting variety; B, raising seedlings: the seeding time is in March, the seeded potatoes are oblique in rows with head up and tail down, plant ash is covered after seeding, fine soil is covered, dual gold herbicide and glyphosate are sprayed on the ridges, and mulching film is coated; C, planting: considering that the blocks in hills and mountains have different sizes, micro-ridge single-row planting is adopted, the planting time of spring potato is in April, the planting time of summer potato is in May, the head and the middle of seedlings are cut, oblique planting is adopted, soil is covered, and moisture supply is maintained after planting; D, applying fertilizer: sweet potato is potassium-liking crop and the applied fertilizer is mainly organic fertilizer; and E, intertilling, weeding and earthing up: intertilling has the effect on breathing and is performed after planting, the depth becomes shallow, and weeding and earthing up are combined with intertilling. The method is easy and simple to operate, adopts micro-ridge single-row planting, efficiently uses the soil, causes the seedbed to be insulated, prompts the potato tubers to sprout quickly and abundantly, simultaneously can inhibit occurrence of black spot, and increases the yield by 20-30%.

The invention belongs to the technical field of animal husbandry, and specifically relates to a method for the ecological captive breeding of goats. According to the method for the ecological captive breeding of goats, a pasture planting base and a sports ground are established, wherein the pasture planting base is used for planting high-yield and high-quality pastures which are used as the feed sources of the captive goats, and the sports ground is used as a sport field for the goats; furthermore, the goat manure and urine in goat pens are collected to make organic fertilizer which is used for the planting fertilization of the pastures; one third of the pasture planting base is used for planting perennial pastures; and two thirds of the pasture planting base is used as an annual pasture planting area which is used for the crop rotation of cold-season and warm-season pastures. According to the method provided by the invention, the obvious conflict of land scramble between the breeding industry and the mountain planting industry is effectively solved and the mountain economic crops are not damaged by the grazed goats any more, so that the safe production is realized, the orderly development of the whole large-scale agriculture is promoted, sustainable development is obtained and eco-friendly development is realized.

The invention provides a ham processing method, comprising the steps of rapid cooling, extruding for blood removal, washing and white wine spraying, condiment applying and kneading, fumigating and hanging for curing. By the method, the ham is free from elevation and region limitations, has less possibility of being infected by maggots, has more beneficial microorganisms breeding inside and has terrific color, aroma and taste.

Provided is a method for manufacturing artificial paving that helps improving global warming. A water permeable paving layer is provided under which a drain layer, which includes gravels or sand, is selectively formed, and an interfacing layer is formed under the drain layer. An ecological gradation layer is set and rammed. The underside ecological gradation layer provides an effect of supporting and, due to the ecological gradation layer containing therein hollow bodies, which can be embodied as disaster-prevention water-storage hollow bodies or earth-improvement hollow bodies or microorganism-culture hollow bodies or water-keeping hollow bodies as desired, allows rainwater falling on ground surface to quickly penetrate down into the underground location, makes the ecological gradation layer effectively preserve water in high water content, and prompt breeding of microorganisms, whereby when the atmosphere is of a high temperature, underground humidity can be released through drainpipes that constitute the water permeable paving.

The invention provides a fluid cooling heat exchanger including: a primary heat exchanger including a closed circuit for circulating cooling fluid; an air cooler located upstream of the primary heat exchanger; a fan arrangement operating to force air through the air cooler and the primary heat exchanger; and a liquid dispenser which operates to dispense liquid into the forced air. The liquid dispenser is located downstream of the air cooler and upstream from the primary heat exchanger. The air cooler includes a moisture absorbent material that is, in use, maintained moist such that air forced through the cooler is cooled by the action of evaporation prior to being forced over a portion of the closed circuit in the primary heat exchanger.

The invention provides a moisture-absorbing and quick-drying antibacterial composite finishing agent, a preparation method and a use method of the moisture-absorbing and quick-drying antibacterial composite finishing agent. The moisture-absorbing and quick-drying antibacterial composite finishing agent comprises the following components in percentage by weight: 0.5-2.0% of moisture-absorbing and quick-drying function polymer, 0.5-1.0% of nano antibacterial powder, 0.3-1.0% of coupling agent, 0.2-1.0% of superdispersant and 95.5-98.0% of solvent. The preparation method comprises the following steps of: chemically modifying and dispersing the nano antibacterial powder by using the coupling agent and superdispersant to prepare a nano antibacterial agent, wherein one end of the nano antibacterial agent is combined by chemical bonds, and the other end of the nano antibacterial agent has a chemical structure of an active hydrogen group or a reaction group; and reacting chemically-modified nano antibacterial powder with modified polyether organosilicon by using the active hydrogen group or the reaction group on a molecular chain, thereby realizing grafting of the nano antibacterial agent and the moisture-absorbing and quick-drying function polymer. A composite finishing liquor is prepared from the finishing agent, a penetrating agent, a softening agent and soft water and is applied to afterfinish of fabrics, and the functional fabrics with antibacterial property, moisture-absorbing property and quick-drying property can be prepared.

The invention discloses a method for treating kitchen waste by using hermetia illucens. The method comprises the following steps: step 1, kitchen waste pretreatment: filtering, sorting, crushing and stacking the kitchen waste; step 2, larva inoculation: flatly laying the pretreated kitchen waste in a waste treatment box, and then inoculating larvae hatched by hermetia illucens eggs into the kitchen waste; and step 3, material and larva separation: collecting and separating mature larvae of the hermetia illucens after breeding treatment and material residues. According to the method for treating the kitchen waste by using the hermetia illucens, the consumption capacity of the kitchen waste is high, the treatment period of the kitchen waste is shortened, breeding of the hermetia illucens larvae is facilitated, and the hermetia illucens larvae can stably and rapidly grow.

The invention discloses a preparation method of a composite functional fresh-keeping paper based material with enhanced slow release function. The preparation method comprises following steps: 1, beta-cyclodextrin and essential oil are weighed to prepare essential oil microcapsules; 2, the essential oil microcapsules and a mesoporous molecular sieve are mixed, water is added, and high speed oscillation treatment is carried out so as to obtain a molecular sieve loaded with the essential oil microcapsules; 4, the molecular sieve loaded with the essential oil microcapsules, a starch solution, a chitosan solution, and an inorganic salt solution are mixed and stirred at a ratio, deionized water is added to adjust the solid content to be 5 to 10% so as to obtain a coating solution; 5, the surface of food package body paper is uniformly coated with the coating solution; and 6, the coated paper is dried fully at room temperature so as to obtain the functional fresh-keeping paper based material. The fresh-keeping paper prepared using the above preparation method is capable of prolonging the storage period of packaged products, and the service life of the fresh-keeping paper can be prolongedat the same time.

The invention, which belongs to the technical field of the aquatic livestock culturing, particularly relates to a four-level culture method of a lateolabrax japonicus pond. First-level cultivation, second-level cultivation, third-level cultivation, and adult fish cultivation are carried out on lateolabrax japonicus in a pond successively. A third-level cultivation pond is encircled by a net and a pond base in a pond; a second-level cultivation pond is encircled by a net and a pond base in the third-level cultivation pond; and a first-level cultivation pond is encircled by a net and a pond base in the second-level cultivation pond. According to the invention, with optimization of the culturing technology in a graded culturing mode, ways containing the culturing means like ecological mixed breeding are improved; the seedlings can be domesticated to eat food in a centralized mode and thus seedlings with uniform sizes can be cultivated; the yield of the fries can be improved; the average acre yield of the lateolabrax japonicus can be increased; and the comprehensive benefits of the lateolabrax japonicus culturing can be improved.