4results about How to "Shorten germination cycle" patented technology

The present application relates to edible mushroom culture identification technical field, specifically a liquid-solid dual-phase coupling culture method of Coriolus hirsutus and application thereof in rapidly identifying correct introduction of Coriolus hirsutus.The method of the present application constructs a liquid-solid dual-phase coupling culture system, combines with sugar agar layer regulation, low-temperature stimulation, etc., realizes rapid determination of Coriolus hirsutus strain characteristics based on the obtained fruiting body, avoids introduction confusion phenomenon, and can also judge strain activity through the fruiting body state, significantly improves the efficiency and benefit of cultivation production and other links.

This invention discloses a method for rapid propagation of *Taxobacterium tumefaciens* tissue culture using a solid-liquid alternation approach. The method includes: pre-treating young stem segments (3-4 cm) from *Taxobacterium tumefaciens* mother plants as explants; inoculating the pre-treated explants into an adventitious bud induction medium for induction culture; inoculating the induced adventitious buds into a proliferation medium for proliferation culture to obtain clustered buds; transferring the clustered buds into a bud strengthening medium for primary bud strengthening culture; and transferring the primary bud strengthening cultured buds into a seedling culture medium for secondary bud strengthening and rooting culture to obtain tissue culture seedlings. The adventitious bud induction medium, proliferation medium, and seedling culture medium are all solid culture media, while the bud strengthening medium is a liquid culture medium. This invention significantly improves the propagation coefficient and shortens the seedling emergence cycle.

The application belongs to the technical field of plant breeding, and particularly relates to a seed pretreatment method and seedling raising method for improving the breeding efficiency of Carpinus putolekii, which comprises the following steps: disinfecting Carpinus putolekii seeds, and performing temperature-variable stratification treatment on the disinfected seeds to obtain pretreated Carpinus putolekii seeds; the temperature-variable stratification treatment comprises: 23-27 DEG C warm treatment for 30 days, and 3-5 DEG C cold treatment for 60 days. The pretreatment method can significantly shorten the germination time of Carpinus putolekii, improve the germination efficiency, and provide technical support for the protection and large-scale seedling raising of the species. The results of the examples show that, by using the method provided in the application, the germination time of Carpinus putolekii seeds is reduced to 14 days, the germination rate is 93.1%, and the seedling rate is 89.7%, so the germination is rapid, and the uniformity is high.

The utility model discloses an agricultural planting germination accelerating device which comprises a water pool, a germination accelerating device body is installed on the inner side of the water pool, a speed adjusting device is arranged on the outer side of the water pool, the speed adjusting device comprises an input pipe, a distribution pipe, an adjusting sleeve, an adjusting plate, an adjusting shaft, an adjusting groove and a movable shaft, the adjusting shaft and the movable shaft are installed on the adjusting plate, and one end of the movable shaft is connected with the distribution pipe; one end of the adjusting shaft is located in the adjusting groove, the adjusting groove is formed in one side of the input pipe, a locking mechanism is installed on the outer side of the distribution pipe and comprises a locking sleeve, a movable plate, a movable hole, a movable rod, a locking block, a locking spring and a locking block, the locking sleeve is installed on the outer side of the distribution pipe in a sliding mode, the movable hole is formed in the movable plate, the movable rod is connected to one side of the locking sleeve, and the locking spring is connected to the locking block. According to the device, full contact between seeds and oxygen and between the seeds and water is guaranteed, and on the basis that the oxygen conveying speed is flexibly adjusted, the stability of a structure after the conveying speed is adjusted is guaranteed.