A seed pretreatment method and seedling raising method for improving the breeding efficiency of caragana jubata

By combining disinfection and temperature-dependent stratification with substrate treatment, the deep dormancy of *Carpinus baohuaensis* seeds was broken, enabling rapid seed germination and efficient propagation. This solved the problems of slow seed germination and low seedling rate, and is suitable for germplasm preservation, population reconstruction, and landscaping.

CN122581052APending Publication Date: 2026-08-18INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Patent Information

Application Number
CN202610934524.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-26
Publication Date
2026-08-18

AI Technical Summary

Technical Problem

The seeds of *Carpinus baohuaensis* have a long germination time and low natural reproduction efficiency. Existing technologies cannot effectively solve the problems of deep dormancy and hard seed coat, resulting in weak regeneration capacity of wild populations.

Method used

After disinfection, the seeds were subjected to a 30-day warm treatment at 23-27℃, followed by a 60-day cold treatment at 3-5℃. This temperature-dependent stratification process, combined with a peat moss, sand, and perlite substrate, was followed by regular application of a fungicide to promote seed germination.

Benefits of technology

It significantly shortens the germination time of *Carpinus baohuaensis* seeds to about 14 days, improves the germination rate and seedling rate, and solves the problems of slow seed germination and low seedling rate. It is suitable for germplasm preservation, population reconstruction and landscaping.

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Abstract

The application belongs to the technical field of plant breeding, and particularly relates to a seed pretreatment method and seedling raising method for improving the breeding efficiency of Carpinus putolekii, which comprises the following steps: disinfecting Carpinus putolekii seeds, and performing temperature-variable stratification treatment on the disinfected seeds to obtain pretreated Carpinus putolekii seeds; the temperature-variable stratification treatment comprises: 23-27 DEG C warm treatment for 30 days, and 3-5 DEG C cold treatment for 60 days. The pretreatment method can significantly shorten the germination time of Carpinus putolekii, improve the germination efficiency, and provide technical support for the protection and large-scale seedling raising of the species. The results of the examples show that, by using the method provided in the application, the germination time of Carpinus putolekii seeds is reduced to 14 days, the germination rate is 93.1%, and the seedling rate is 89.7%, so the germination is rapid, and the uniformity is high.
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