A method for rapid asexual propagation of a new variety of ginger flower

Abstract

This invention discloses a method for the rapid asexual propagation of a new ginger lily variety, comprising the following steps: obtaining rhizomes; treating with gibberellin, stacking several layers of rhizomes in a frame by layering them with wet straw, and then placing the frame containing the rhizomes and straw in a dark environment to promote germination; removing rhizomes with sprouting buds, quickly dipping them in a chlorpyrifos solution, and then cultivating them in a shaded area with humidity, allowing the stem buds to grow and differentiate into root tip tissue; uniformly spraying a growth regulator solution onto the surface of the rhizomes and stem buds, cultivating them in a shaded area with humidity, allowing the stem buds to grow into seedlings, dividing the rhizomes to obtain seedlings with complete root systems, and transplanting them; neatly arranging the transplanted seedlings on a seedbed in a shaded area, cultivating them with humidity, watering with a hymexazol solution at regular intervals, spraying foliar fertilizer when the seedlings develop leaves, and transplanting or releasing them from the nursery when the leaf and stem height reaches the standard. Advantages include: balancing the speed and seedling survival rate of asexual propagation of ginger lily, improving the propagation coefficient and seedling quality of the 'Huayao' ginger lily.

Description

Technical Field

[0001] This invention relates to the field of horticultural seedling propagation technology, and in particular to the asexual propagation technology of ginger flower varieties. Background Technology

[0002] Hedychium coronarium, commonly known as ginger lily, is a perennial herbaceous plant belonging to the genus Hedychium in the family Zingiberaceae. Its stems grow to 1-2 meters tall, with terminal spike-like inflorescences bearing white, fragrant, and beautiful flowers that bloom from July to November. A characteristic flower of South China, ginger lily is an excellent material for landscaping, potted plants, and cut flowers. It is commonly cultivated for ornamental purposes and has high economic value.

[0003] 'Huayao' ginger lily is a new evergreen variety developed through artificial hybridization, using 'H. menghaiense' 'MH-1' (female parent) and 'H. coronarium' 'BJH-1' (male parent) as the female parent (variety right number: Yuepinghua 20210001). This variety has large and full inflorescences, high uniformity of flowering, a group flowering period from mid-June to mid-November, a delicate fragrance, and strong growth.

[0004] 'Huayao' ginger lily has a low natural seed setting rate and unstable flowering characteristics, making it difficult to propagate by seed. In production, asexual propagation through rhizome division is used. 'Huayao' ginger lily has robust rhizomes, and when dividing, the rhizomes are divided into units of 2-3 leaf stems (or residual stem stubble). Division propagation is fast and has a high seedling rate. However, the number of 'Huayao' ginger lily seed stems is limited and the division propagation coefficient is low, making it difficult to meet the needs of propagating high-quality 'Huayao' ginger lily seedlings. Summary of the Invention

[0005] The purpose of this invention is to provide a method for the rapid asexual propagation of a new variety of ginger flower, in order to solve the problem of the low propagation coefficient of the 'Huayao' ginger flower by division in the prior art.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A method for the rapid asexual propagation of a new variety of ginger flower includes the following steps:

[0008] (1) Obtain the rhizome;

[0009] (2) Rhizome sprouting treatment: Gibberellin treatment, several layers of rhizomes are piled in a frame with a layer of wet straw on top of the rhizomes, and then the frame containing the rhizomes and straw is placed in a dark environment to promote sprouting, so that plump buds sprout from the internodes of the rhizomes.

[0010] (3) Stem bud differentiation induction: Take out the rhizomes that have sprouted buds, dip them quickly in chlorpyrifos solution, and then cultivate them in a shaded greenhouse with moisture. The stem buds grow and differentiate into root tip tissue.

[0011] (4) Seedling cultivation and division and planting: The growth regulator solution is evenly sprayed onto the surface of the rhizome and stem buds, and then the seedlings are cultivated in a shaded shed with moisture. The stem buds grow into seedlings. The rhizome is divided into units of 1 or 2 seedlings to obtain seedlings with rhizomes and complete root systems, and then planted.

[0012] (5) Cultivating strong seedlings: Arrange the transplanted seedlings neatly on the seedbed in the shade shed, keep them moist, water them with hymexazol solution every once in a while, spray foliar fertilizer when the seedlings unfold their leaves, and transplant or leave the nursery when the leaf and stem height reaches the standard.

[0013] Furthermore, the process of obtaining the rhizome in step (1) includes: from December to February of the following year, select a healthy one-year-old 'Huayao' ginger flower, dig up the underground rhizome completely, shake off the soil, cut off all the leaf stems and roots from the base of the rhizome, leaving no short stem or root stubble, and then wash the rhizome clean.

[0014] Furthermore, the detailed process of step (2) of rhizome sprouting treatment includes: soaking the rhizomes in a 10-20 mg / L gibberellin solution for 15 minutes, taking out the rhizomes and draining the surface water, soaking the straw in clean water for 30 minutes and draining it until no water drips out, first laying a layer of rhizomes flat at the bottom of the basket, covering it with a layer of wet straw, stacking several layers of rhizomes in the basket in the manner of covering one layer of rhizomes with one layer of wet straw, and then placing the basket containing the rhizomes and straw in a dark indoor environment at 25-35°C for 7 days to sprout, so that plump buds sprout from the internodes of the rhizomes.

[0015] Furthermore, in step (2), each layer of rhizomes is covered with a 2-3 cm thick layer of wet straw, and 3-4 layers of rhizomes are piled inside the frame.

[0016] Furthermore, the detailed process of inducing stem-bud differentiation in step (3) includes: taking out rhizomes with sprouting buds, quickly dipping them in 20-30 mg / L chlorpyrifos solution, and then placing them on a sand bed in a shaded shed with a shading rate of 70-80%, and culturing them at 18-30°C with humidity, so that the stem-buds grow and differentiate into root tip tissue.

[0017] Furthermore, in step (3), the quick dipping process involves immersing the budding rhizomes in a 20-30 mg / L chlorpyrifos solution for 3-5 seconds and then quickly removing them; the shade shed moisturizing method is to spray mist water once each in the morning, noon and evening, with each spray lasting 2-4 minutes, and the cultivation time is 7 days.

[0018] Further, the detailed process of step (4) of seedling cultivation and division and planting includes: uniformly spraying the surface of the rhizome and stem buds with growth regulator solution, and then cultivating them in a sand bed in a shaded shed with a shading rate of 70-80% at 18-30℃ with humidity. The stem buds grow into seedlings. The rhizome is divided into units of 1 or 2 seedlings to obtain seedlings with rhizomes and complete root systems. The length of the rhizome cut at the base of the seedling is 1.2-2.0cm or 2.5-4.0cm. The cut is coated with powdered yellow soil. The seedlings with rhizomes and complete root systems are planted in the culture medium and thoroughly watered.

[0019] Furthermore, in step (4), the method for maintaining humidity in the shade is as follows: spray mist water once each in the morning, noon, and evening, with each spray lasting 2-4 minutes, and the cultivation period is 7 days.

[0020] The growth regulator solution contains 10-20 mg of chlorpyrifos, 10-20 mg of naphthaleneacetic acid (IAA), and the remainder of water per 1 L of solution. The culture medium is a mixture of general nutrient soil, perlite, and bio-organic fertilizer in a volume ratio of 7:2:1.

[0021] Furthermore, the detailed process of the seedling cultivation in step (5) includes: neatly placing the transplanted seedlings on the seedbed in the shade shed, adjusting the shading rate of the shade shed to 40-60%, keeping the seedlings moist, watering with hymexazol solution once every once in a while, spraying urea fertilizer on the leaves once every once in a while when the seedlings unfold their leaves, spraying continuously for 3-4 times, and transplanting or leaving the nursery when the average height of the leaf stem exceeds 40cm.

[0022] Furthermore, the method for maintaining humidity in the shade structure in step (5) is as follows: spray mist water once each in the morning, noon, and evening, with each spray lasting 3-6 minutes.

[0023] Water the seedlings with a 30% hymexazol solution diluted 1500 times every 20 days. When the seedlings have 2-3 leaves, spray the leaves with urea fertilizer with a mass fraction of 0.2-0.4% every 7 days, and spray for 3-4 consecutive times.

[0024] The advantages of this invention include:

[0025] 1. This invention uses the robust rhizomes of the new ginger flower variety 'Huayao' for asexual propagation, which can well preserve the excellent traits of 'Huayao' ginger flower. Moreover, the stem bud induction and seedling cultivation speed is fast, and the seedling rate and seedling quality are high. Under the premise that the number of seed stems of the new variety is limited, the method of this invention can also multiply the propagation coefficient and seedling speed, effectively meeting the breeding needs of high-quality seedlings of new ginger flower varieties.

[0026] 2. This invention uses a medium-low concentration of gibberellin solution to soak the rhizomes and an indoor "high temperature and medium humidity" environment for germination treatment, which can effectively induce the germination of dormant buds in the rhizomes. The germination method is simple and efficient, suitable for the batch processing of new ginger flower varieties. The germination time is fast, and plump buds can be seen sprouting from the internodes of the rhizomes in just 7 days.

[0027] 3. This invention effectively promotes bud enlargement by rapidly dipping the buds sprouting from the rhizomes in a medium concentration of chlorpyrifos solution. Chlorpyrifos is a plant growth regulator with high cytokinin activity, which can accelerate cell mitosis, promote cell enlargement and differentiation, thereby affecting bud development. After 7 days of cultivation in a high-temperature and high-humidity environment under a shade structure in spring, the buds rapidly developed into small stem buds with an average length exceeding 2.0 cm. Some stem buds even differentiated root tips at their base, laying a good foundation for the rapid development of subsequent seedlings.

[0028] 4. This invention uses a low-concentration chlorpyrifos solution for spraying to continuously induce the development of small stem buds, and combines this with a medium concentration of auxin to regulate the growth of the stem buds. After 7 days of induction culture in a high-humidity environment under shade, the average length of the seedlings exceeds 5cm and differentiates into 1-4 tender roots, with an average root system of 2.83 roots. In the later stage of seedling differentiation culture, the seedlings are further divided, and each rhizome can be separated to obtain 9-14 seedlings with small rhizomes and complete root systems, resulting in a high propagation coefficient. The small rhizomes, as vegetative organs, can directly provide nutrients for the growth of the seedlings. At the same time, the complete root system facilitates the absorption of water and nutrients from the substrate, allowing the seedlings to grow rapidly after transplanting without the need for a acclimatization period. In addition, the mixed substrate used in this invention is nutrient-rich, water-retentive, and breathable, and is also rich in beneficial microorganisms that activate the root environment, making it very suitable for the transplanting, rooting, and nutrient absorption of ginger flower seedlings, promoting rapid root development and accelerating seedling formation.

[0029] 5. This invention employs targeted water and fertilizer measures to cultivate robust seedlings. By reducing the shading rate of the shade structure, the adaptability of seedlings to light is improved, and seedling height and leaf expansion are promoted. Appropriate frequency of spray irrigation can continuously replenish the water needed for seedling growth and prevent root rot or disease infection due to water accumulation. The subsequent foliar nitrogen fertilizer topdressing can quickly replenish the nutrients needed for growth and promote plant tillering. After 3-4 consecutive sprays, the average number of suckers per plant reaches 2.3, and the average height of the leaf stem exceeds 40cm.

[0030] This invention optimizes the rhizome division propagation of ginger lily. Given the limited number of rhizomes for new ginger lily varieties, it balances the speed and seedling rate of asexual propagation while significantly increasing the propagation coefficient and seedling quality of the 'Huayao' ginger lily. The method is simple and efficient, featuring innovative stem-bud differentiation and seedling induction culture with strong continuity. It is easy to operate, has low cultivation costs, and a propagation coefficient as high as 11.3, 3.4 times that of conventional division propagation. Transplanting can begin after 21 days of continuous cultivation. Further water and fertilizer treatment promotes strong seedling growth, resulting in rapid plant growth, high seedling quality, and a seedling rate of up to 96%. The seedling cycle is only 8-9 weeks, 1-4 weeks shorter than conventional division propagation. This method is suitable for large-scale seedling production of new ginger lily varieties in conventional production, offering advantages such as a high propagation coefficient, short seedling cycle, and low production costs. It enables rapid and large-scale propagation of new ginger lily varieties, meeting the strong demand for new and superior ginger lily seedlings in the landscaping market. Attached Figure Description

[0031] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this application, do not constitute an undue limitation of the invention. In the drawings:

[0032] Figure 1 These are experimental photos of the 'Huayao' rhizomes after pruning.

[0033] Figure 2 These are photos of an experiment on the rapid germination of root and stem buds induced by gibberellin;

[0034] Figure 3 These are experimental photos of different stages in which buds develop into stem buds and begin to differentiate into adventitious roots.

[0035] Figure 4 These are experimental photos of seedlings that were cut after they grew to more than 5cm in length;

[0036] Figure 5 These are experimental photos of seedling cultivation and leaf expansion.

[0037] Figure 6 These are experimental photos of seedlings being cultivated into high-quality large seedlings. Detailed Implementation

[0038] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. The illustrative embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0039] Example 1

[0040] This embodiment provides a method for the rapid asexual propagation of a new variety of ginger flower, the method comprising the following steps:

[0041] I. Selection of Rhizomes

[0042] From December to February of the following year, select healthy one-year-old 'Huayao' ginger flowers, dig up the underground rhizomes completely, shake off the soil, and cut off all the leaves, stems, and roots from the base of the rhizome, leaving no short stem or root stubble. This yields the rhizome. Figure 1 As shown, rinse the roots and stems thoroughly with clean water.

[0043] II. Rhizome Sprouting Treatment

[0044] (1) Gibberellin treatment: Soak the prepared rhizomes in a 10-20 mg / L gibberellin (GA3) solution for 15 minutes, then remove the rhizomes and drain the surface water;

[0045] (2) Sprouting method: Use plastic baskets and wet straw to stack the rhizomes in layers. The straw should be soaked in clean water for 30 minutes and drained slightly until no water drips. First, place a layer of rhizomes flat at the bottom of the plastic basket, and cover it with a layer of wet straw 2-3 cm thick. Then stack 3-4 layers of rhizomes in the manner of "one layer of rhizomes + one layer of wet straw".

[0046] (3) Sprouting environment: Place the plastic baskets filled with rhizomes in a dark indoor environment, and use air conditioning or other temperature control equipment to maintain the room temperature at 25-35℃ day and night. After 7 days of sprouting, if... Figure 2 As shown, plump buds can sprout from the internodes of the rhizome.

[0047] III. Induction of stem-bud differentiation

[0048] (1) Stalk and bud induction: In order to accelerate the germination of the rhizome buds, the rhizome was taken out of the plastic basket and immersed in 20-30 mg / L chlorpyrifos (CPPU) solution for 3-5 seconds, and then quickly taken out.

[0049] (2) Cultivation Environment: The above-mentioned rhizomes were cultivated in a shade house equipped with an automatic misting system, with a shading rate of 70-80%. The treated rhizomes were placed flat on a sand bed inside the shade house, and the humidity inside was maintained by misting. Depending on the weather conditions, misting water was sprayed three times a day, morning, noon, and evening, for 2-4 minutes each time, maintaining the temperature inside the shade house at 18-30℃. After 7 days of continuous cultivation, the average length of the stem buds exceeded 2.0 cm, and root tip tissue began to differentiate from the base of some stem buds. Stem buds at different stages were cut off, arranged, and photographed, such as... Figure 3 As shown, the development and rooting process of the bud can be demonstrated.

[0050] IV. Seedling Cultivation and Division Planting

[0051] (1) Seedling culture: In order to accelerate the growth and development of stems and buds, a growth regulator solution was sprayed evenly on the surface of the rootstock and stems and buds. The growth regulator solution contained 10 mg of chlorpyrifos, 20 mg of naphthaleneacetic acid (IAA) and the remainder of water per 1 L of solution. The culture environment was the same as that for stem and bud differentiation induction. After 7 days, the average length of the seedlings exceeded 5.0 cm and the average number of roots was 2.83.

[0052] (2) Sprout division: Using individual sprouts as units, the rhizome is divided to obtain sprouts with rhizomes and complete root systems. The length of the rhizome cut at the base of the sprout is 1.2–2.0 cm. The cut surface is coated with powdered yellow clay. The cut surface is then photographed with the photograph facing downwards. Figure 4 As shown, each rhizome can be divided to obtain an average of 11.3 seedlings with their own small rhizome pieces and complete root systems.

[0053] (3) Seedling transplanting: Use plastic flower pots or nutrient pots with a diameter of 10-15cm to cultivate the above-mentioned seedlings with rhizomes and complete root systems. The culture medium is general nutrient soil, perlite and biological organic fertilizer. After mixing evenly in a volume ratio of 7:2:1, carefully transplant the seedlings with roots into the flower pots, planting 1-2 seedlings in each pot, and then water thoroughly.

[0054] V. Cultivation of Strong Seedlings

[0055] (1) Cultivation environment: Arrange the transplanted seedlings neatly on the seedbed in the shade shed, and adjust the shading rate of the shade shed to 40-60%; thereafter, use spraying to replenish water, spraying mist water once in the morning, noon and evening every day, depending on the dryness and wetness of the weather, each spraying lasts for 3-6 minutes, and watering once every 20 days with a 30% hymexazol solution diluted 1500 times to prevent root and stem diseases and rot.

[0056] (2) Foliar fertilization: After 1-2 weeks of cultivation, when the seedlings have 2-3 leaves, Figure 5 As shown, spray the leaves with a 0.2-0.4% urea solution every 7 days, repeating 3-4 times. Transplant or remove from the nursery when the average stem height exceeds 40cm. Figure 6 As shown, the seedling survival rate reached 96%.

[0057] Example 2: Effect of bud-inducing methods on root and stem bud germination

[0058] To investigate the effects of different germination methods on the germination of rhizome buds in 'Huayao' ginger flower, this example tested three different germination methods, specifically:

[0059] A1: In a well-ventilated shade structure, layer the rhizomes with moist river sand, keeping the sand moisture content between 15% and 25%. First, spread a 3-4cm thick layer of moist river sand on the seedbed in the shade structure, then place a layer of rhizomes on top. Repeat this process, layering the rhizomes with a layer of moist river sand followed by a layer of rhizomes, for a total of 3-4 layers. Check the sand pile and rhizome budding every 3 days, and water the soil promptly when the sand moisture content decreases.

[0060] A2: At room temperature, use dry cardboard boxes and old newspapers to stack the rhizomes in layers. First, place a layer of rhizomes flat at the bottom of the cardboard box, and then cover it with a layer of crumpled newspaper. Then, stack 3 to 4 layers of rhizomes in the manner of "one layer of rhizomes + one layer of crumpled newspaper". Check the cardboard box every 3 days to observe the bud sprouting of the rhizomes.

[0061] A3: In a dark indoor environment, use plastic baskets and wet straw to layer the rhizomes. The straw should be soaked in clean water for 30 minutes and then drained slightly until no water drips. First, place a layer of rhizomes flat at the bottom of the plastic basket, and then cover it with a 2-3cm thick layer of wet straw. Then, layer the rhizomes 3-4 times in the manner of "one layer of rhizomes + one layer of wet straw". Use air conditioning or other temperature control equipment to maintain the room temperature at 25-35℃ day and night. Check the germination of the rhizomes in the basket every 3 days.

[0062] Fifteen rhizomes were treated with each of the above sprouting methods. After 10 days of sprouting, the germination rate was determined by the diameter of dormant buds greater than 3 mm. The germination status of the buds was observed and statistically analyzed, and SPSS 21.0 was used for significance analysis. The results are as follows:

[0063]

[0064] Note: Different letters within the treatment indicate significant differences at the 0.05 level, and the same applies below.

[0065] Statistical analysis shows that treatment A1 had 1 rotten rootstock and 14 preserved rootstocks. Treatment A3 had the best sprouting effect, with intact rootstocks and no rot as seen in treatment A1. It also had the highest total number of sprouting buds, reaching 123, meaning an average of 8.2 sprouting buds per rootstock, significantly better than treatments A1 and A2. This indicates that the high temperature and humidity environment of treatment A3 was most conducive to the sprouting of 'Huayao' ginger flowers.

[0066] Example 3: Effect of Gibberellin Treatment on Rhizome Bud Germination

[0067] To investigate the effect of different concentrations of gibberellin (GA3) on the germination of rhizomes of 'Huayao' ginger flower, this example tested four different concentrations: B1: 5 mg / L, B2: 10 mg / L, B3: 20 mg / L, and B4: 40 mg / L, with water as a control (CK: 0 mg / L). The prepared rhizomes were soaked in each gibberellin solution for 15 minutes, then removed and drained of surface water, and germination was promoted according to the optimal method in Example 2.

[0068] Ten rhizomes were treated with each of the above concentrations. After 7 days of germination, the germination rate was determined by the diameter of dormant buds greater than 3 mm. The germination status of the buds was observed and statistically analyzed using SPSS 21.0. The results are as follows:

[0069]

[0070] Statistical analysis shows that the average number of sprouts from the rhizomes in the gibberellin-treated groups was significantly higher than that in the control group. With increasing gibberellin concentration, the number of sprouts from the rhizomes of 'Huayao' ginger flowers initially increased and then decreased. Treatment B3 showed the best effect, with an average of 11.2 sprouts, 3.4 more than the control group; B2 was second best, with little difference from B3; B4 had the highest gibberellin concentration, but the number of sprouts decreased significantly, and a number of deformed sprouts appeared. This indicates that high concentrations of gibberellin can inhibit sprouting. Therefore, it can be concluded that a gibberellin concentration of 10–20 mg / L is most beneficial for the sprouting of 'Huayao' rhizomes.

[0071] It should be noted that in this embodiment, obvious germination of root and stem buds can be seen in only 7 days, which is 3 days earlier than the 10 days of treatment A3 in Example 2. It can be seen that the germination treatment method in this embodiment not only produces more buds but also germinates faster.

[0072] Example 4: Effect of chlorpyrifos treatment on bud differentiation

[0073] To accelerate rhizome bud differentiation, the highly effective plant growth regulator chlorpyrifos (CPPU) was used for rapid dipping of rhizomes to investigate the effect of different concentrations of chlorpyrifos on the bud differentiation of 'Huayao' ginger flower rhizomes. This example tested four different concentrations of chlorpyrifos (CPPU) solutions: C1: 0 mg / L, C2: 10 mg / L, C3: 20 mg / L, and C4: 30 mg / L. Rhizomes treated with the optimal bud-promoting treatment in Example 3 were removed from the plastic basket and immersed in the aforementioned chlorpyrifos solutions for 3–5 seconds, then quickly removed and allowed to dry slightly.

[0074] The above-mentioned rhizomes were cultivated in a shade house equipped with an automatic misting system, with a shading rate of 70-80%. The treated rhizomes were placed flat on a sand bed inside the shade house, and the humidity inside the house was maintained by misting. Depending on the dryness and humidity of the weather, mist water was sprayed once in the morning, noon and evening every day, with each spray lasting 2-4 minutes, to maintain the temperature inside the house at 20-30℃.

[0075] Ten rhizomes were treated with each of the above concentrations and cultured for 7 consecutive days. The development of stem buds was measured and analyzed, and SPSS 21.0 was used for significance analysis. The results are as follows:

[0076]

[0077] Statistical analysis shows that, compared with the control group C1, treatments with different concentrations of chlorpyrifos promoted bud differentiation, resulting in larger bud diameters and significantly increased stem and bud length. The promoting effect showed a clear positive correlation as the chlorpyrifos concentration increased from 0 to 30 mg / L. Specifically, at a concentration of 30 mg / L, the average stem and bud diameter reached the maximum of 7.43 mm, and the average stem and bud length was the longest, reaching 1.61 cm. Treatments C3 and C4 showed very similar effects, with little difference in average stem and bud diameter and length. Furthermore, some stem and bud bases began to differentiate into root tips, which is highly beneficial for subsequent stem and bud growth and development. Therefore, it can be concluded that a chlorpyrifos concentration of 20–30 mg / L has the best promoting effect on the differentiation of root and stem buds in 'Huayao' ginger flower.

[0078] Example 5: Effects of plant growth regulators on seedling growth

[0079] To promote stem and bud elongation and root development, a solution containing plant growth regulators was sprayed onto the surface of rhizomes and stem buds to investigate the effects of different combinations of the auxin naphthaleneacetic acid (NAA) and cytokinin chlorpyrifos (CPPU) on the growth of 'Huayao' ginger flower seedlings. This example tested five different combinations: D1: CPPU 20 mg / L, D2: NAA 20 mg / L, D3: CPPU 10 mg / L + NAA 10 mg / L, D4: CPPU 10 mg / L + NAA 20 mg / L, and D5: CPPU 20 mg / L + NAA 10 mg / L. The above solutions were uniformly sprayed onto the surface of the rhizomes and stem buds of the optimal treatment in Example 4, and the culture environment was the same as the shaded spray culture in Example 4.

[0080] Ten rhizomes with buds obtained from the optimal treatment in Example 4 were treated with each of the above concentrations. After continuous culture for 7 days, the growth and development of the buds were measured and statistically analyzed, and significance analysis was performed using SPSS 21.0. The results are as follows:

[0081]

[0082]

[0083] Statistical analysis shows that, based on the optimal treatment in Example 4, the D1 treatment, with additional spraying of chlorpyrifos solution, significantly promoted the height growth of seedlings. The D2 treatment, with only naphthaleneacetic acid (NAA) solution sprayed, showed a significant effect on promoting root development. In treatments D3–D5, the different concentrations of NAA and chlorpyrifos had varying effects on seedling growth, with average seedling length exceeding 5 cm and average root count exceeding 2. Treatments D4 and D5 showed the best effect on promoting average seedling length, with little difference between the two, but the average number of roots in D4 was significantly higher than in D5, reaching a maximum of 2.83. Treatments D4 and D2 showed the most significant effect on promoting the number of roots, with no significant difference between the two, but the average seedling length in D4 was significantly higher than in D2, reaching 5.86 cm. In summary, treatment D4 effectively promoted both seedling elongation and root development, making it the most suitable hormone treatment combination.

[0084] Example 6: Effects of different cutting methods on seedling transplantation

[0085] To investigate the effects of different cutting methods on seedling transplantation and growth, this example tested three different cutting methods on seedlings that had undergone the optimal treatment in Example 5: E1: carefully cutting the tender seedlings with roots directly from the rhizome, without the original rhizome; E2: cutting the rhizome into individual stem buds to obtain seedlings with rhizomes and complete root systems, with the rhizome cut length at the base of the seedling being 1.2–2.0 cm; E3: cutting the rhizome into two stem buds to obtain seedlings with rhizomes and complete root systems, with the rhizome cut length at the base of the seedling being 2.5–4.0 cm; all cuts were coated with powdered yellow soil.

[0086] Cultivate the cut seedlings in plastic flower pots or nutrient pots with a diameter of 10-15cm. The culture medium is general nutrient soil, perlite, and bio-organic fertilizer. Mix them evenly in a volume ratio of 7:2:1. Carefully plant the rooted seedlings obtained by the above cutting method into the flower pots, planting 1-2 seedlings in each pot, and then water them thoroughly.

[0087] Ten rhizomes with buds were treated using each of the above cutting methods. After four weeks of routine transplanting and care, the seedling growth rate was measured and statistically analyzed, and SPSS 21.0 was used for significance analysis. The results are as follows:

[0088]

[0089]

[0090] Statistical analysis shows that the three different cutting methods have varying effects on seedling transplant growth. Regarding survival rate, E2 and E3 seedlings, due to their small rhizomes and complete root systems, achieved 100% survival. E1 seedlings, lacking small rhizomes, had more delicate wound tissue, making them susceptible to infection or rot, resulting in a survival rate of only 62.7%. In terms of propagation coefficient, E2, with its smallest cutting size, yielded the most rooted seedlings from the original rhizome, achieving a propagation coefficient as high as 10.9, almost twice that of E3. E1, affected by the survival rate, had an average coefficient of only 6.9. Regarding average plant height and leaf number, E2 and E3, with their small rhizomes and complete root systems, had average plant heights exceeding 30cm and average leaf numbers exceeding 3. Furthermore, E3's larger rhizomes provided more nutrients for seedling growth, resulting in better average plant height and leaf number compared to E2, although the differences were not statistically significant.

[0091] In summary, both E2 and E3 have a 100% transplant survival rate and similar plant height and leaf quantity. However, the propagation coefficient of E2 is almost twice that of E3. Given the limited number of stem cuttings for new ginger lily varieties, E2 is more conducive to the propagation of ginger lily seedlings and obtaining a larger number of seedlings. Therefore, E2 can be considered the most suitable stem-bud division method for new ginger lily varieties.

[0092] Example 7: Effects of different robust seedling cultivation methods on the growth of ginger lily seedlings

[0093] To promote the robust growth of transplanted seedlings, this example conducted three water and fertilizer-based seedling cultivation experiments on the seedlings of the optimal treatment in Example 6, namely:

[0094] F1: Arrange the seedlings neatly on the seedbed inside the shade structure, maintaining a shading rate of 70-80%. Water by spraying, depending on the weather conditions, spraying a fine mist three times a day (morning, noon, and evening) for 2-4 minutes each time. When the seedlings have 2-3 leaves, apply 1g of urea fertilizer evenly to the substrate surface every 10-15 days, repeating this fertilization 2-3 times.

[0095] F2: Arrange the seedlings neatly on the seedbed inside the shade structure, adjusting the shade ratio to 40-60%. Water by spraying, depending on the weather conditions, spraying a fine mist three times a day (morning, noon, and evening) for 2-4 minutes each time. When the seedlings have 2-3 leaves, apply a 0.4-0.6% urea solution evenly to the substrate surface every 7-10 days, repeating this fertilization 2-4 times.

[0096] F3: Arrange the seedlings neatly on the seedbed inside the shade structure, adjusting the shade ratio to 40-60%. Water by spraying, depending on the weather conditions, spraying a fine mist three times a day (morning, noon, and evening) for 3-6 minutes each time. Water with a 1500-fold dilution of 30% hymexazol every 20-30 days. When the seedlings have 2-3 leaves, spray the leaves with a 0.2-0.4% urea-soluble fertilizer every 7 days, repeating 3-4 times.

[0097] Fifty seedlings were treated with each of the above water and fertilizer-based seedling cultivation methods. After 4 weeks of cultivation, the growth and development of the seedlings in each treatment were statistically analyzed, and significance analysis was performed using SPSS 21.0. The results are as follows:

[0098]

[0099] Statistical analysis shows that different water and fertilizer cultivation methods have significant effects on seedling growth. Among them, F3 seedlings have the best cultivation effect, with a seedling survival rate of up to 98% and an average plant height of over 40cm, which is significantly better than F1 and F2. The average number of offshoots is 2.3, which is slightly less than F2, but the difference between the two is not significant.

[0100] It should be noted that F1 seedlings have excessively high shading rates, resulting in insufficient light, weak and elongated stems, and require hardening off before being transplanted. F2 seedlings have reduced shading rates but insufficient water supply, making them prone to leaf tip curling or scorching during the hot spring days. F3 seedlings have reduced shading rates while extending the spraying time. Combined with foliar nitrogen fertilizer, this effectively replenishes the water and nutrients needed for seedling growth, promotes tillering, and results in thicker stems and stronger seedlings.

[0101] Example 8: Comparison of the method of the present invention with conventional division propagation

[0102] To illustrate the innovative effects of this invention, the method in this embodiment uses the optimal treatment combination of Examples 2-7 and is compared with the conventional ginger flower leaf and stem division propagation method, as detailed below:

[0103] G1: This refers to the conventional rhizome division method, as detailed below:

[0104] I. Seed Stem Treatment: From December to February of the following year, select healthy one-year-old 'Huayao' ginger flowers, dig up the underground rhizomes intact, shake off the soil, and cut off more than 90% of the total length of the leaf stems and excessively long old roots and messy roots; cut the seed stems into units of 2-3 remaining stem stubble and retain intact buds.

[0105] 2. Seedling transplanting: Use garden soil and 15-20cm diameter nutrient pots to cultivate the above-mentioned seed stems. Carefully transplant the seed stems into the nutrient pots, one seed stem per pot. Arrange the transplanted nutrient pots neatly on the seedbed, and then water thoroughly with a 1000-1500 times dilution of 70% hymexazol or 75% chlorothalonil to settle the roots.

[0106] III. Water and Fertilizer Management: Keep the potting soil moist by watering manually or by automatic sprinkler irrigation; after the stem buds emerge 10cm from the soil, spray with a 0.3-0.5% urea solution every 10-15 days for 3-4 consecutive fertilizations to promote leaf and stem growth and tillering; transplant or remove from the nursery when the average height of the leaf and stem exceeds 40cm.

[0107] G2: The method of this invention adopts the optimal combination of Examples 2-7;

[0108] Sixty plants were treated with each of the two propagation methods, and the treatments were repeated in three groups. After the average stem height exceeded 40 cm, the seedling emergence rate and seedling cycle were recorded for both methods. The results are compared below:

[0109]

[0110] Statistical analysis shows that the asexual propagation of the new 'Huayao' ginger lily using this invention outperforms conventional division propagation methods in terms of propagation coefficient, seedling rate, average number of tillers, seedling cycle, and seedling quality. Specifically, the propagation coefficient of this invention is 3.4 times that of conventional division propagation; the seedling rate of both methods is above 90%, but the seedling rate of this invention is higher, reaching over 96%; the average number of tillers in this invention is slightly higher than that of conventional division, and the seedling cycle is shorter, requiring only 8-9 weeks of cultivation, with an average stem height exceeding 40cm, 1-4 weeks earlier than conventional division; the seedling quality of this invention is high, with robust stems, bright green leaves, and no obvious pests or diseases. Therefore, this invention not only balances the seedling rate and quality of 'Huayao' ginger lily but also significantly improves its propagation coefficient and seedling speed.

[0111] The technical solutions provided by the embodiments of the present invention have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of the embodiments of the present invention. The descriptions of the embodiments above are only for helping to understand the principles of the embodiments of the present invention. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the embodiments of the present invention. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. A method for the rapid asexual propagation of a new variety of ginger flower, characterized in that: Includes the following steps: (1) Obtaining rhizomes; (2) Rhizome sprouting treatment: 10-20 mg / L gibberellin treatment, pile several layers of rhizomes in a basket by covering each layer with a layer of wet straw, and then place the basket containing the rhizomes and straw in a dark environment to promote sprouting, so that plump buds sprout from the internodes of the rhizomes. (3) Stem-bud differentiation induction: Take out the rhizomes that have sprouted buds, quickly dip them in 20-30 mg / L chlorpyrifos solution, and then keep them moist in a shaded greenhouse with a shading rate of 70-80% at 18-30℃. The stem-buds will grow and differentiate into root tip tissue. (4) Seedling culture and division and planting: The growth regulator solution is evenly sprayed on the surface of the rhizome and stem buds, and then cultured in a shaded shed with a shading rate of 70-80% at 18-30℃ with humidity. The stem buds grow into seedlings. The rhizome is divided into units of 1 or 2 seedlings to obtain seedlings with rhizomes and complete root systems, and then planted. The growth regulator solution contains 10-20 mg of chlorpyrifos, 10-20 mg of naphthaleneacetic acid and the remainder of water per 1 L of solution. (5) Cultivating strong seedlings: Arrange the transplanted seedlings neatly on the seedbed in a shade shed with a shading rate of 40-60%, keep them moist, water them with hymexazol solution every once in a while, spray foliar fertilizer when the seedlings unfold their leaves, and transplant them when the leaf and stem height reaches the standard.

2. The method for rapid asexual propagation of a new ginger flower variety according to claim 1, characterized in that: The process of obtaining the rhizome in step (1) includes: from December to February of the following year, select a healthy one-year-old 'Huayao' ginger flower, dig up the underground rhizome completely, shake off the soil, cut off all the leaf stems and roots from the base of the rhizome, leaving no short stem or root stubble, and then wash the rhizome clean.

3. The method for rapid asexual propagation of a new ginger flower variety according to claim 1, characterized in that: The detailed process of step (2) of the rhizome sprouting treatment includes: soaking the rhizomes in gibberellin solution for 15 minutes, taking out the rhizomes and draining the surface water, soaking the straw in clean water for 30 minutes and draining it until no water drips out, first laying a layer of rhizomes flat at the bottom of the basket, covering it with a layer of wet straw, stacking several layers of rhizomes in the basket in the manner of covering one layer of rhizomes with one layer of wet straw, and then placing the basket containing the rhizomes and straw in a dark indoor environment at 25-35℃ for 7 days to sprout, so that plump buds sprout from the internodes of the rhizomes.

4. The method for rapid asexual propagation of a new ginger flower variety according to claim 3, characterized in that: In step (2), each layer of rhizomes is covered with a 2-3 cm thick layer of wet straw, and 3-4 layers of rhizomes are piled in the basket.

5. The method for rapid asexual propagation of a new ginger flower variety according to claim 1, characterized in that: In step (3), the rapid dipping process involves immersing the budding rhizomes in a 20-30 mg / L chlorpyrifos solution for 3-5 seconds and then quickly removing them. The shaded shed moisture retention method is to spray mist water once each in the morning, noon and evening, with each spray lasting 2-4 minutes, and the cultivation time is 7 days.

6. The method for rapid asexual propagation of a new ginger flower variety according to claim 1, characterized in that: The detailed process of step (4) of seedling cultivation and division and planting includes: uniformly spraying the surface of the rhizome and stem buds with growth regulator solution, then cultivating them in a sand bed in a shaded area with moisture, and the stem buds growing into seedlings. The rhizome is divided into units of 1 or 2 seedlings to obtain seedlings with rhizomes and complete root systems. The length of the rhizome cut at the base of the seedling is 1.2-2.0 cm or 2.5-4.0 cm. The cut is coated with powdered yellow soil. The seedlings with rhizomes and complete root systems are planted in the culture medium and thoroughly watered.

7. The method for rapid asexual propagation of a new ginger flower variety according to claim 6, characterized in that: The method for maintaining humidity in the shade structure during step (4) is as follows: spray mist water once each in the morning, noon, and evening, with each spray lasting 2-4 minutes, and the cultivation period is 7 days. The culture medium is made by mixing general nutrient soil, perlite, and bio-organic fertilizer in a volume ratio of 7:2:

1.

8. The method for rapid asexual propagation of a new ginger flower variety according to claim 1, characterized in that: The detailed process of step (5) of cultivating strong seedlings includes: neatly placing the transplanted seedlings on the seedbed in the shade shed, keeping them moist, watering them with hymexazol solution once every once in a while, spraying the leaves with urea water-soluble fertilizer once every once in a while when the seedlings unfold their leaves, spraying them 3 to 4 times in a row, and transplanting them when the average height of the leaf stem exceeds 40 cm.

9. The method for rapid asexual propagation of a new ginger flower variety according to claim 8, characterized in that: The method for maintaining humidity in the shade structure in step (5) is as follows: spray mist water once each in the morning, noon, and evening, with each spray lasting 3-6 minutes. Water the seedlings with a 30% hymexazol solution diluted 1500 times every 20 days. When the seedlings have 2-3 leaves, spray the leaves with a 0.2-0.4% urea water-soluble fertilizer every 7 days for 3-4 consecutive times.

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