17results about How to "Increase biomass" patented technology

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

This invention belongs to the field of microbial technology, specifically relating to a strain of Acetobacter peroxide 672 and its applications. The Acetobacter peroxide 672 strain of this invention can enhance the antagonistic activity of Pichia pastoris against Rhizoctonia solani and also antagonizes Colloidal anthracnose, providing a multi-faceted solution for the biological control of crop diseases. Furthermore, the strain possesses the ability to produce indoleacetic acid, which can significantly promote the growth and development of grain crops such as wheat and corn, promote root expansion, enhance nutrient and water absorption, and thus effectively increase biomass. In addition, the strain can tolerate and degrade chemical pesticide components such as imidacloprid and carbendazim, alleviating soil pesticide residue pollution, and can effectively alleviate the inhibitory effects of low pH stress on crops, enhancing their stress resistance. Therefore, the Acetobacter peroxide 672 strain of this invention has significant application value in the biological control of crop diseases, crop growth promotion, soil pesticide residue remediation, and enhancing crop stress resistance.

This invention discloses a method for topping tobacco leaves in cigar tobacco cultivation. This method ensures uniform growth of tobacco plants in the field, regulates nicotine content in the leaves, and improves yield and overall usability. The method includes the following steps: S1, Timing Determination: The optimal time for topping is determined when the tobacco plant has 14-16 leaves, with the top leaf reaching 2-3 cm in length; S2, Topping Operation: The top growing point of the tobacco plant is removed; S3, Post-processing: The removed growing point is collected and disposed of outside the field. Topping can improve tobacco quality, promote leaf growth and biomass, thereby increasing yield; it can make leaves thicker, more oily, and better colored; it can also effectively regulate the content of nicotine and other chemical components in the tobacco leaves, making them more balanced, thus improving intrinsic quality; and it can also improve the overall usability of the tobacco leaves.

This invention provides a *Roseolariomoraxella salina* strain for saline-alkali soil improvement, with accession number CGMCC No. 36067. The *Roseolariomoraxella salina* strain provided by this invention exhibits significant comprehensive application advantages. This strain demonstrates excellent survival and growth capabilities under saline-alkali stress, effectively reducing salinity and alkalinity in saline-alkali soils and thus improving the saline-alkali environment. Whole-genome analysis shows that this strain carries multiple functional genes related to osmotic regulation and ion transport, providing a molecular basis for its salinity-reducing ability. Adding citric acid or betaine can further enhance its salinity-reducing performance, and its biomass is significantly increased after optimization of fermentation conditions. This strain shows promising application prospects in saline-alkali soil improvement and saline-alkali aquaculture environment management.

The application discloses a plant growth regulator. By externally applying the plant growth regulator to leguminous plants, the leguminous plants can maintain high tolerance under weak light conditions such as dense planting, strip complex planting, under forest, facility greenhouse or cloudy and rainy days, and the implementation is simple, labor is saved, and the plant growth regulator can be used for mechanical operation.

This invention belongs to the field of genetic engineering technology, specifically involving TaSCAMP17 The application of its recombinant overexpression vector in improving plant salt tolerance, the TaSCAMP17 The nucleotide sequence is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention will... TaSCAMP17 Functional validation was performed in Arabidopsis thaliana after overexpression, and the results showed that overexpression... TaSCAMP17 The salt tolerance of wild-type Arabidopsis thaliana is significantly improved, therefore TaSCAMP17 It plays a positive regulatory role in wheat salt tolerance, providing a theoretical basis and gene resources for wheat molecular breeding, and is of great significance for obtaining salt-tolerant and high-yielding lines and improving wheat salt tolerance in the future.

The application discloses a low-carbon solidified soil matrix and application. The matrix is made of dredged soil, biochar, slag and gypsum mixed in a specific ratio, and the preferred ratio (T1) is that the mass ratio of the dredged soil, the biochar, the slag and the gypsum is 60:15:10:15. By comparing the mechanical properties and the plant growth effects of different ratios (CK, T1, T2 and T3), it is found that the unconfined compressive strength of the T3 ratio (with the highest content of slag) is the highest (up to more than 3000kPa) after 60 days; however, the T1 ratio (with the highest content of biochar) exhibits the best ecological effect, including significantly promoting the growth of ryegrass, increasing the biomass, optimizing the carbon-nitrogen ratio of soil and enhancing the soil microbial activity, while maintaining sufficient engineering strength (about 1000kPa after 60 days) and excellent dry-wet cycle performance. The application realizes the balance between the mechanical and ecological restoration functions through the collaborative utilization of multi-source solid wastes, and provides an economic, environment-friendly and efficient solution for erosion areas.

The application provides application of OpCBR1 transcription factor in improving growth of short small snake grass hairy roots and increasing camptothecin yield, and the application comprises the following steps: constructing a plant expression vector containing an OpCBR1 gene, and genetically transforming short small snake grass stem section explants to obtain short small snake grass hairy roots over-expressing the OpCBR1 gene; using a weighing method and a high performance liquid chromatography method to analyze the biomass and the camptothecin yield of the transgenic hairy roots, and the results show that over-expression of the OpCBR1 gene can improve the biomass of the short small snake grass hairy roots, thereby improving the camptothecin yield; through dynamic observation, it is found that the biomass of the hairy roots of the transgenic strain of OpCBR1 is significantly improved on the 16th day, and reaches the maximum on the 32nd day, and the yield also reaches the maximum, which indicates that over-expression of the OpCBR1 gene can obviously shorten the culture period. The application provides a new high-quality drug source with broad-spectrum anticancer effect for producing camptothecin, and has important theoretical significance and potential application value.

This invention discloses a method and its application for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites by adding carrageenan to a semi-solid culture medium. The method uses *Propionibacterium f. reudenreichii* subsp. *shermanii* NBRC No. 12426 as the experimental strain and *Pichia anomala* SICC 2.894 as the indicator strain. It discloses a method for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites against the indicator strain *Pichia anomala* by adding carrageenan to the culture medium. Based on the culture conditions and method of *Propionibacterium f. reudenreichii* subsp. *shermanii* in this invention, the optimal amount of carrageenan added is selected as 0.6%, which significantly enhances the antibacterial activity of the *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites.

The application provides a treatment method for promoting seed germination of Carex gynaecorum and Carex muliensis, and relates to the technical field of wet meadow population propagation. The treatment method is to set different soil burial depths and cover the community litter of the wet meadow on the soil surface to promote the seed germination of Carex gynaecorum and Carex muliensis. The application overcomes the defects of the prior art, can improve the germination rate of the seeds of Carex gynaecorum and Carex muliensis to a certain extent, promotes the growth of Carex gynaecorum and Carex muliensis, and provides a direction for artificial breeding.

This invention relates to a compound with resistance-inducing and growth-promoting effects and its application in pesticide-fertilizer systems. The main active ingredient of the compound is methyl ferulic acid, with an effective concentration of 0.05 μg / mL to 0.5 μg / mL, used to induce plant disease resistance and promote plant growth. This invention also discloses the application of methyl ferulic acid in pesticide-fertilizer systems, comprising pesticide active ingredients, methyl ferulic acid, wetting agents, dispersants, thickeners, defoamers, stabilizers, and antifreeze agents; the effective concentration of methyl ferulic acid is 0.05 μg / mL to 0.5 μg / mL. This invention combines pesticide activity with nutrient supply, forming a dual-effect composition of "disease prevention + nutrition," solving the cumbersome problem of multiple applications of pesticides and fertilizers in traditional agriculture, significantly reducing the number of applications and labor costs, and improving field management efficiency.

The application relates to a lighting device and method suitable for greenhouse cultivation, which is arranged in a greenhouse for mixed cultivation of animals and plants. The device comprises a carbon dioxide supply structure for supplementing gas carbon dioxide or organic carbon fertilizer to a plant area; a light source for providing light required by plants for growth; a photosynthesis monitoring unit provided with a first detection unit for detecting a net photosynthetic rate of the plants and a second detection unit for collecting leaf area of the plants; and a control unit in communication connection with the carbon dioxide supply structure, the light source and the photosynthesis monitoring unit. One of the purposes of the application is to provide a system for solving the problem of carbon hunger of plants, which comprises an above-ground carbon amount detection module and an underground carbon amount supply module. The control unit can obtain the carbon amount required to be supplied to the plants by the underground carbon amount supply module based on the total carbon amount required by the plant growth and the carbon conversion amount of the plants under photosynthesis detected by the above-ground carbon amount detection module according to the real-time change of illumination.

This invention provides a *Trichoderma guiyuan* strain B10 and its applications, relating to the field of agricultural microbial control technology. The *Trichoderma guiyuan* strain B10 is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 3.25634. This invention overcomes the shortcomings of existing technologies. This strain can inhibit the pathogenic fungi *Neoplasmosis polychaete*, *Neoplasmosis polychaete*, *Neoplasmosis spp.*, *Botrytis cinerea*, and *Metacarpus* spp. of garlic leaf spot disease, while simultaneously promoting the growth of garlic plants, exhibiting both disease resistance and growth-promoting functions in seedlings.

This invention discloses a gene, PvHsf16, encoding a plant heat shock transcription factor, and its applications. This gene originates from differentially expressed genes upregulated in members of the Hsf gene family of switchgrass under cadmium (Cd) stress. By constructing a yeast expression vector for PvHsf16, PvHsf16 cells exhibited significantly greater cadmium tolerance, indicating that this gene enhances yeast cadmium resistance. PvHsf16 is located in the cell nucleus. Overexpression of the PvHsf16 gene in Arabidopsis thaliana, compared to the wild type under CdCl2 treatment, showed enhanced cadmium tolerance in the PvHsf6 overexpressing lines, with significantly increased chlorophyll content, root length, and biomass. The PvHsf16 gene provides a genetic resource for cultivating switchgrass germplasm suitable for both energy grass and cadmium remediation, and can be applied to the remediation of cadmium-polluted areas, playing an important role in researching phytoremediation technologies and improving soil heavy metal pollution.

This invention provides the application and method of Streptomyces S16-1 in promoting the growth and salt stress tolerance of Leymus chinensis, belonging to the field of microbial technology. Streptomyces S16-1 was isolated from saline soil, with the preservation number CCTCC M 20251940, and its 16S rDNA sequence is shown in SEQ ID NO:1. The Streptomyces S16-1 has functional activities such as salt and alkali tolerance, phosphorus solubilization, nitrogen fixation, and production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. The microbial fertilizer is obtained by expanding the culture of Streptomyces S16-1. Compared with chemical fertilizers, the microbial fertilizer used in this invention is more environmentally friendly. It can not only improve soil fertility and help plants improve their salt and alkali resistance, but also promote the balance and diversity of soil microbial communities, which helps maintain the health and stability of the soil ecosystem.

A strain of *Aureobacterium wanduense* producing pectinase and its applications, characterized in that: the strain is named 159, and its classification is named... Chryseobacterium wanjuense The specimen, with accession number CGMCC No. 37744, is deposited at the China General Microbiological Culture Collection Center. This invention also provides its optimized culture method and application technology for pectin degradation in aged tobacco, addressing the problems of low pectin degradation efficiency and lack of specific functional strains in existing tobacco aging processes. Compared with existing technologies, this invention has the following outstanding advantages: it is the first to screen and verify the ability of *Aureobacterium wanduense* strain 159 to efficiently degrade pectin in aged tobacco, expanding the application scope of *Aureobacterium* in tobacco processing and providing the tobacco industry with a novel microbial resource and green processing technology.

This utility model relates to the field of wastewater purification technology, specifically a wastewater purification device for immobilized beneficial microorganisms and algal symbiosis. It includes a floating box with a floating mechanism, an installation groove at the top, a mesh frame on the groove, a microbial immobilization carrier filled in the mesh frame, a woven nylon mesh at the top, and microalgae loaded inside the mesh. A through-slot is located at the bottom of the floating box. This structure, through the synergistic effect of bacteria and algae, can effectively remove organic matter and simultaneously remove eutrophic factors such as nitrogen and phosphorus. It also has a certain adsorption and enrichment effect on heavy metals, improving the overall wastewater treatment effect, reducing the pressure on subsequent deep treatment stages, and lowering the overall treatment cost.