44results about How to "Increase biomass" patented technology

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

This invention belongs to the field of microbial technology, specifically relating to a strain of Acetobacter peroxide 672 and its applications. The Acetobacter peroxide 672 strain of this invention can enhance the antagonistic activity of Pichia pastoris against Rhizoctonia solani and also antagonizes Colloidal anthracnose, providing a multi-faceted solution for the biological control of crop diseases. Furthermore, the strain possesses the ability to produce indoleacetic acid, which can significantly promote the growth and development of grain crops such as wheat and corn, promote root expansion, enhance nutrient and water absorption, and thus effectively increase biomass. In addition, the strain can tolerate and degrade chemical pesticide components such as imidacloprid and carbendazim, alleviating soil pesticide residue pollution, and can effectively alleviate the inhibitory effects of low pH stress on crops, enhancing their stress resistance. Therefore, the Acetobacter peroxide 672 strain of this invention has significant application value in the biological control of crop diseases, crop growth promotion, soil pesticide residue remediation, and enhancing crop stress resistance.

The invention discloses a microbial compound fermentation inoculant, a straw fermentation product and application of the straw fermentation product, and belongs to the technical field of agricultural microorganisms. The preparation method comprises the following steps: compounding bacillus subtilis, trichoderma harzianum and trichoderma longibrachiatum to form a microbial compound fermentation inoculant; when the microbial compound fermentation inoculant is used for fermenting ginger straws, the content of pyrogallic acid in a prepared straw fermentation product can be synergistically improved. The pyrogallic acid has an obvious antibacterial effect on fusarium moniliforme and fusarium laminatum which cause the apple continuous cropping obstacle; the growth of apple seedlings planted in continuous cropping soil can be effectively promoted. Therefore, the straw fermentation product disclosed by the invention can be used for preventing and treating apple continuous cropping obstacles, improving the biomass and root activity of apple seedlings under continuous cropping conditions and reducing the quantity of harmful fusarium in continuous cropping soil.

The invention discloses a polyamine carbon dot as well as a preparation method and application thereof in improvement of corn resistance, and belongs to the technical field of nano agricultural regulation and control. The polyamine carbon dots are subjected to hydrothermal synthesis, the size of the PEICDs is 2.3 nm, the quantum yield of the carbon dots can be increased, carbon dioxide in the atmosphere can be captured and converted into bicarbonate radicals, and it is verified through in-vitro experiments that the PEICDs can improve the carbon dioxide conversion product 3-phosphoglycerate in vitro; under the stress of drought and salt, the polyethyleneimine functionalized carbon dot solution is applied to the leaf surfaces of the crops, so that the growth and development of the crops can be improved by remarkably improving the net photosynthetic rate and the intercellular carbon dioxide concentration, and the adaptability of the crops to the environment is further improved.

This invention discloses a method for topping tobacco leaves in cigar tobacco cultivation. This method ensures uniform growth of tobacco plants in the field, regulates nicotine content in the leaves, and improves yield and overall usability. The method includes the following steps: S1, Timing Determination: The optimal time for topping is determined when the tobacco plant has 14-16 leaves, with the top leaf reaching 2-3 cm in length; S2, Topping Operation: The top growing point of the tobacco plant is removed; S3, Post-processing: The removed growing point is collected and disposed of outside the field. Topping can improve tobacco quality, promote leaf growth and biomass, thereby increasing yield; it can make leaves thicker, more oily, and better colored; it can also effectively regulate the content of nicotine and other chemical components in the tobacco leaves, making them more balanced, thus improving intrinsic quality; and it can also improve the overall usability of the tobacco leaves.

This invention discloses a salt-tolerant Cladosporium 550W1 strain and its application in promoting nodulation and nitrogen fixation in leguminous plants, belonging to the field of microbial technology. The salt-tolerant Cladosporium (… Cladosporium halotolerans Cladosporium 550W1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 41943, provides a salt-tolerant Cladosporium 550W1 that can significantly promote nodulation and nitrogen fixation in legumes, increase plant biomass and nitrogen accumulation, and reduce dependence on chemical nitrogen fertilizers. It has broad application prospects and environmental and ecological benefits.

The invention provides a culture medium for lactic acid bacteria after-generation and application, the culture medium takes trehalose as a carbon source and a yeast extract as a nitrogen source, so that the growth biomass of lactobacillus plantarum, lactobacillus casei and lactobacillus pentosus can be remarkably increased, the pH is reduced, and the antibacterial ability is improved. Lactobacillus plantarum, lactobacillus casei and lactobacillus pentosus are prepared through the culture medium for the lactobacillus after-generation, so that the lactobacillus after-generation has a good inhibition effect on main food-borne pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella, pseudomonas aeruginosa and listeria monocytogenes; the preservative can be widely applied to preservation and preservation of meat products such as chilled chicken and pork. The preparation method of the lactic acid bacteria metagen is simple, convenient, low in cost, natural, safe and suitable for large-scale production.

The application provides a method for improving the LPS stress resistance of broilers, which is realized by feeding a mannose oligosaccharide compound preparation prepared from enzymolysis coffee grounds. The preparation method of the mannose oligosaccharide compound preparation comprises the following steps: crushing coffee grounds, and adding a compound reagent for pretreatment; adding a compound enzyme preparation I to the mixed system after reaction, and carrying out enzymolysis for 6-8 hours under the condition of 50-60 DEG C; then adding a compound enzyme preparation II, and carrying out sufficient enzymolysis under the condition of 55-65 DEG C; after the enzymolysis is completed, a fermentation compound microbial agent is used for continuing fermentation for 6-12 hours under the condition of 30-35 DEG C, and drying is carried out; the compound enzyme preparation I comprises papain, alpha-amylase and saccharifying enzyme; the compound enzyme preparation II comprises cellulase, mannanase and glucanase; and the fermentation compound microbial agent comprises maxwell kluyver yeast and lactobacillus plantarum. The method can not only avoid drug residues caused by feeding drugs, but also realize the reuse of coffee grounds.

The invention relates to a method for promoting growth, nodulation and nitrogen fixation of indigofera pseudotinctoria by utilizing rhizobium D303 and a bacterial solution thereof. The biological preservation number of the rhizobium sp. D303 is CCTCC (China Center for Type Culture Collection) NO: M20252803. Pot culture tie-back tests prove that the indigofera pseudotinctoria seedlings inoculated with the rhizobium are obviously superior to a control group in the aspects of plant height, root length, overground dry weight, underground dry weight, root nodule number, root nodule weight, root nodule diameter, nitrogenase activity, antioxidant enzyme activity and the like. The rhizobium strain provided by the invention has efficient nodulation and nitrogen fixation capability and excellent growth promoting characteristic, obviously improves the growth speed, nodulation capability and nitrogen fixation efficiency of the indigofera pseudotinctoria, reduces the dependence on chemical nitrogen fertilizer, and has a broad application prospect in development of special biological bacterial fertilizer for indigofera pseudotinctoria. The method has important ecological and economic benefits for promoting the application of the indigofera pseudotinctoria in ecological restoration and feed production, and is suitable for slope greening, water and soil conservation and barren mountain ecological restoration projects.

The invention discloses a method for treating sewage by combining micro-aerobic hydrolysis with coenzyme catalyst reinforced activated sludge. The method adopts a pretreatment tank, a primary sedimentation tank, an aerobic tank and a secondary sedimentation tank which are sequentially connected in series, according to the method, activated sludge containing microorganisms is put into a pretreatment tank and an aerobic tank, aeration is carried out while the pretreatment tank is filled with filler, a sludge domestication stage is firstly carried out, a coenzyme catalyst is put into the aerobic tank when the treatment capacity of a system reaches the optimum, modified activated sludge is obtained, and the sewage treatment efficiency is further improved. The coenzyme catalyst contains a carrier and an iron source; the carrier is selected from at least one of activated carbon and diatomite. The method is simple to operate, convenient to manage and suitable for upgrading and reconstruction of industrial wastewater.

This invention provides a *Roseolariomoraxella salina* strain for saline-alkali soil improvement, with accession number CGMCC No. 36067. The *Roseolariomoraxella salina* strain provided by this invention exhibits significant comprehensive application advantages. This strain demonstrates excellent survival and growth capabilities under saline-alkali stress, effectively reducing salinity and alkalinity in saline-alkali soils and thus improving the saline-alkali environment. Whole-genome analysis shows that this strain carries multiple functional genes related to osmotic regulation and ion transport, providing a molecular basis for its salinity-reducing ability. Adding citric acid or betaine can further enhance its salinity-reducing performance, and its biomass is significantly increased after optimization of fermentation conditions. This strain shows promising application prospects in saline-alkali soil improvement and saline-alkali aquaculture environment management.

The application discloses a plant growth regulator. By externally applying the plant growth regulator to leguminous plants, the leguminous plants can maintain high tolerance under weak light conditions such as dense planting, strip complex planting, under forest, facility greenhouse or cloudy and rainy days, and the implementation is simple, labor is saved, and the plant growth regulator can be used for mechanical operation.

The present application relates to edible mushroom culture identification technical field, specifically a liquid-solid dual-phase coupling culture method of Coriolus hirsutus and application thereof in rapidly identifying correct introduction of Coriolus hirsutus.The method of the present application constructs a liquid-solid dual-phase coupling culture system, combines with sugar agar layer regulation, low-temperature stimulation, etc., realizes rapid determination of Coriolus hirsutus strain characteristics based on the obtained fruiting body, avoids introduction confusion phenomenon, and can also judge strain activity through the fruiting body state, significantly improves the efficiency and benefit of cultivation production and other links.

The invention relates to the technical field of microalgae culture devices, in particular to an efficient microalgae culture device and a using method thereof.The efficient microalgae culture device comprises a first-stage raceway pond, a second-stage raceway pond and a third-stage raceway pond which are sequentially stacked from bottom to top, and a partition plate is arranged at the bottom of a pond body of each stage of raceway pond; the horizontal areas of the three layers of raceway ponds are sequentially reduced from bottom to top, and a first-stage stirring system, a second-stage stirring system and a third-stage stirring device are arranged at one time corresponding to each stage of raceway pond; wherein each of the first-stage stirring system and the second-stage stirring system comprises a stirring device and a drainage device, the drainage device comprises a backflow channel and a drainage pipeline, and a lighting system is arranged at the bottom of each stage of raceway pond; the raceway ponds are arranged on the basis of a multi-layer stacking mode, and the effective culture liquid volume in unit occupied area is effectively increased; meanwhile, through the drainage mechanism, culture solution flow inoculation in the multi-stage raceway pond is achieved, the gas-liquid mass transfer efficiency is improved, the inoculation amount and the initial algae cell concentration are greatly improved, the culture period is shortened, and the culture efficiency is improved.

The application provides a composition containing arbuscular mycorrhizal fungi (AMF) and biochar and application thereof in improving tobacco cadmium tolerance and / or improving soil, and belongs to the technical field of bio-agriculture. The application can effectively promote tobacco growth, improve tobacco agronomic traits and biomass, effectively improve tobacco cadmium tolerance, effectively reduce cadmium accumulation in tobacco, change the subcellular distribution of cadmium in tobacco, convert high-toxicity chemical state cadmium into medium-toxicity or low-toxicity cadmium, and is also beneficial to improving soil environment and nutrient regulation antioxidant enzyme activity, and provides a better soil environment for tobacco growth.

This invention belongs to the field of genetic engineering technology, specifically involving TaSCAMP17 The application of its recombinant overexpression vector in improving plant salt tolerance, the TaSCAMP17 The nucleotide sequence is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention will... TaSCAMP17 Functional validation was performed in Arabidopsis thaliana after overexpression, and the results showed that overexpression... TaSCAMP17 The salt tolerance of wild-type Arabidopsis thaliana is significantly improved, therefore TaSCAMP17 It plays a positive regulatory role in wheat salt tolerance, providing a theoretical basis and gene resources for wheat molecular breeding, and is of great significance for obtaining salt-tolerant and high-yielding lines and improving wheat salt tolerance in the future.

This invention belongs to the field of genetic engineering technology, specifically relating to a CtBBX4 protein, gene, and applications related to plant salt tolerance. This invention provides a CtBBX4 protein related to plant salt tolerance, the amino acid sequence of which is shown in SEQ ID NO.1. The CtBBX4 protein of this invention can improve the salt tolerance of plants. Examples show that overexpression of the CtBBX4 protein in plants... CtBBX4 Genes can increase plant germination rate and biomass, and improve plant salt tolerance by enhancing antioxidant capacity. CtBBX4 Genes are constructed into plant expression vectors and then genetically transformed using Agrobacterium-mediated transformation, which can effectively obtain new germplasm with salt tolerance characteristics, and is of great significance for the breeding of salt-tolerant crops.

The application discloses a low-carbon solidified soil matrix and application. The matrix is made of dredged soil, biochar, slag and gypsum mixed in a specific ratio, and the preferred ratio (T1) is that the mass ratio of the dredged soil, the biochar, the slag and the gypsum is 60:15:10:15. By comparing the mechanical properties and the plant growth effects of different ratios (CK, T1, T2 and T3), it is found that the unconfined compressive strength of the T3 ratio (with the highest content of slag) is the highest (up to more than 3000kPa) after 60 days; however, the T1 ratio (with the highest content of biochar) exhibits the best ecological effect, including significantly promoting the growth of ryegrass, increasing the biomass, optimizing the carbon-nitrogen ratio of soil and enhancing the soil microbial activity, while maintaining sufficient engineering strength (about 1000kPa after 60 days) and excellent dry-wet cycle performance. The application realizes the balance between the mechanical and ecological restoration functions through the collaborative utilization of multi-source solid wastes, and provides an economic, environment-friendly and efficient solution for erosion areas.

The application provides application of OpCBR1 transcription factor in improving growth of short small snake grass hairy roots and increasing camptothecin yield, and the application comprises the following steps: constructing a plant expression vector containing an OpCBR1 gene, and genetically transforming short small snake grass stem section explants to obtain short small snake grass hairy roots over-expressing the OpCBR1 gene; using a weighing method and a high performance liquid chromatography method to analyze the biomass and the camptothecin yield of the transgenic hairy roots, and the results show that over-expression of the OpCBR1 gene can improve the biomass of the short small snake grass hairy roots, thereby improving the camptothecin yield; through dynamic observation, it is found that the biomass of the hairy roots of the transgenic strain of OpCBR1 is significantly improved on the 16th day, and reaches the maximum on the 32nd day, and the yield also reaches the maximum, which indicates that over-expression of the OpCBR1 gene can obviously shorten the culture period. The application provides a new high-quality drug source with broad-spectrum anticancer effect for producing camptothecin, and has important theoretical significance and potential application value.

The application belongs to the technical field of genetic engineering, and discloses a method for improving biomass and cell protein content of Bacillus amyloliquefaciens by knocking out global transcription factor codY. The engineering strain is obtained by taking Bacillus amyloliquefaciens HZ-12 as a starting bacterium and knocking out the global transcription factor coding gene codY, and the biomass and cell protein content of the Bacillus amyloliquefaciens engineering bacterium HZ-12ΔcodY are increased by 37.44% and 6.88% respectively relative to those of the control strain HZ-12.

The invention belongs to the field of soil pollution treatment, and relates to a method for jointly relieving toxicity of microplastics to a soil-plant system based on a nitrification inhibitor-carbon nanomaterial. The method comprises the following steps: applying a nitrification inhibitor and a carbon nanomaterial to plant planting soil; the nitrification inhibitor is DMPP, and the carbon nanomaterial is one or more of a multi-walled carbon nanotube and a nanodiamond. The method can technically slow down microplastic pollution, improve soil health and guarantee quality safety of agricultural products.

This invention discloses a method and its application for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites by adding carrageenan to a semi-solid culture medium. The method uses *Propionibacterium f. reudenreichii* subsp. *shermanii* NBRC No. 12426 as the experimental strain and *Pichia anomala* SICC 2.894 as the indicator strain. It discloses a method for enhancing the antibacterial activity of *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites against the indicator strain *Pichia anomala* by adding carrageenan to the culture medium. Based on the culture conditions and method of *Propionibacterium f. reudenreichii* subsp. *shermanii* in this invention, the optimal amount of carrageenan added is selected as 0.6%, which significantly enhances the antibacterial activity of the *Propionibacterium f. reudenreichii* subsp. *shermanii* metabolites.

The application provides a treatment method for promoting seed germination of Carex gynaecorum and Carex muliensis, and relates to the technical field of wet meadow population propagation. The treatment method is to set different soil burial depths and cover the community litter of the wet meadow on the soil surface to promote the seed germination of Carex gynaecorum and Carex muliensis. The application overcomes the defects of the prior art, can improve the germination rate of the seeds of Carex gynaecorum and Carex muliensis to a certain extent, promotes the growth of Carex gynaecorum and Carex muliensis, and provides a direction for artificial breeding.

The utility model discloses a kind of disturbance type sewage treatment equipment based on microbial inoculant processing, it is related to sewage treatment technical field, including processing box, the inoculant delivery assembly is arranged on the upper surface of processing box, the oxygen delivery assembly is arranged on the lower surface of processing box, the stirring assembly is arranged in the inside of processing box;The stirring assembly includes growth frame, the growth frame is arranged in the inside of processing box, a plurality of connecting rods are fixedly connected in the middle of growth frame, the utility model provides a large number of attachment growth surface area for microorganism by setting built-in growth frame's stirring assembly, effectively improve the biomass in reactor, enhance system processing capacity and stability;The design of unique hyperboloid vertical shaft stirring disc is matched with the same direction circular arc disturbance strip, can produce soft and multi-dimensional three-dimensional flow field under the driving of submersible motor.

The present application relates to a kind of yunnan ganoderma strain HL45 and its cultivation method and application, belong to the field of microbial technology.The strain has been preserved in Guangdong Provincial Microbial Culture Collection Center on September 11, 2024, and the preservation number is GDMCC No:65119.The present application yunnan ganoderma strain HL45 fruiting body presents single or multi-branch, cap purple brown, flesh white, and stable in character;The content of triterpenoid ganoderol F and glucosan polysaccharide is very high, which can be used for preparing antitumor drugs.In addition, the fruiting body of the strain has strong mushroom aroma, and the quality of the whole mushroom is good.The average growth cycle of cultivation bag is 26d, the mycelial growth rate is 0.78cm / d, the average yield is 16.62g / bag, the highest biological efficiency reaches 10.26%, the cultivation period is about 4-5 months, and it is suitable for cultivation in high-altitude areas above 2000m, with high ornamental and application value.

This invention relates to a compound with resistance-inducing and growth-promoting effects and its application in pesticide-fertilizer systems. The main active ingredient of the compound is methyl ferulic acid, with an effective concentration of 0.05 μg / mL to 0.5 μg / mL, used to induce plant disease resistance and promote plant growth. This invention also discloses the application of methyl ferulic acid in pesticide-fertilizer systems, comprising pesticide active ingredients, methyl ferulic acid, wetting agents, dispersants, thickeners, defoamers, stabilizers, and antifreeze agents; the effective concentration of methyl ferulic acid is 0.05 μg / mL to 0.5 μg / mL. This invention combines pesticide activity with nutrient supply, forming a dual-effect composition of "disease prevention + nutrition," solving the cumbersome problem of multiple applications of pesticides and fertilizers in traditional agriculture, significantly reducing the number of applications and labor costs, and improving field management efficiency.

This invention belongs to the field of microbiology and relates to a lysobacterium P18 and its applications. Lysobacterium P18 belongs to the genus *Lysobacter* and was deposited with CGMCC on June 7, 2024, with accession number NO. 30877. The lysobacterium P18 provided by this invention has a growth-promoting effect on tomatoes.

The application discloses a coal gangue-based sandy soil modifier and application thereof, and belongs to the technical field of soil improvement. The modifier is composed of a basic component and a composite component, wherein the basic component is coal gangue, and the composite component is composed of straws and rotten leaves. The mass percentage of the basic component is 1-5%, and the mass ratio of the straws to the rotten leaves in the composite component is (1-4):(1-4). The modifier provided by the application can simultaneously, quickly and durably increase the contents of organic matter, total nitrogen and total phosphorus in sandy soil, significantly improve the problems of loose structure, poor water and fertilizer retention capacity and extreme nutrient deficiency of the sandy soil, and improve the soil fertility from an extremely low level to a high level, thereby effectively overcoming the problems of single effect, poor nutrient synergy, short duration and high cost of the existing modifier. The modifier is suitable for ecological restoration and fertility improvement of flowing sandy land, semi-fixed sandy land and sandy farmland.

The invention discloses application of nano-zinc oxide in improving biomass and active ingredient content of liquorice, relates to the technical field of plant cultivation, and aims to improve biomass and active ingredient content of liquorice. By applying nano zinc oxide externally, the plant height, root length and dry matter accumulation amount of the liquorice can be remarkably increased, and the biomass of the liquorice is increased; meanwhile, synthesis and accumulation of main active ingredients such as glycyrrhizic acid and liquiritin are effectively promoted; meanwhile, the physiological status of liquorice under stress conditions such as salt stress can be improved, the stress resistance is enhanced, and the in-vivo ion balance and the redox steady state are regulated, so that the growth and quality of liquorice are synergistically improved.

The present application relates to a kind of aerobic granular sludge and its fast culture method, including (1) in reactor inoculation aerobic activated sludge, using low ammonia nitrogen high COD culture solution is cultivated under low dissolved oxygen condition, obtain functional aerobic sludge;(2) in step (1) system, add culture solution and regulator, and inoculate functional inoculant, cultivate under high-low dissolved oxygen alternation condition, obtain primary granular sludge;(3) primary granular sludge is cultivated using culture solution containing chitosan, obtain final aerobic granular sludge.The present application can realize the rapid formation of granular sludge, the adaptability of the obtained granular sludge is strong, functional flora is stable, and long-period operation effect is good, and can be used for the biochemical treatment process of various toxic or refractory organic pollutants, nitrogen and phosphorus containing industrial wastewater.