57 results about "Schizochytrium sp." patented technology

The invention discloses a Schizochytrium sp., which has a category name of Schizochytrium sp., is preserved in Common Microorganisms Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CCTCC No.209059. The invention also discloses a method for producing DHA lipa by using the Schizochytrium sp. The method optimizes a strain fermentation medium from the perspectives of osmotic pressure and element supply and combines a fed-batch strategy to achieve high-density fermentation of microalgae, thus the final dry cell weight reaches 70 grams per liter, the lipa content reaches 31.5 grams per liter, and the DHA accounts for more than 35 percent of the total fatty acid content. All indexes of the DHA essential oil obtained by the method accord with the standard of food additives, and the DHA essential oil contains a bioactive substance of squalene. The method has the advantages of simple entire process, convenient operation, and high biomass and DHA content, reduces the fermentation cost, and is suitable for industrial production.

The invention discloses a Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and a fermentation method thereof. The Schizochytrium sp.TIO1101 strain provided by the invention has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.4603. After the Schizochytrium sp.TIO1101 is subjected to high-density heterotrophic fermentation for 72h, the biomass liveweight, the lipid content and the DHA are respectively 120g/L, 54.3% and 51.4% of the content of total lipid, therefore the Schizochytrium sp.TIO1101 has development and application values extremely. The Schizochytrium sp.TIO1101 disclosed by the invention has the advantages that: the Schizochytrium sp.TIO1101is obtained through separation; the high-density heterotrophic fermentation of the Schizochytrium sp.TIO1101 is realized; and the lipid extracted from the Schizochytrium sp.TIO1101 cells obtained under the fermentation condition has high DHA content and purer components.

The invention relates to a preparation method of DHA (which relates to an unsaturated fatty acid. The invention provides a preparation method of a fully synthetic medium that is suitable for high density growth of fissiparism chytrid and increases the output of the DHA. The DHA comprises a carbon source, amino acids, lysine, inorganic salts, vitamins, trace elements and water. The carbon source is glucose, the amino acids comprise alanine, methionine, cysteine, lysine, histidine, glutamic acid, glutamine, isoleucine, threonine and tryptophan; the vitamins comprise VB1, VB12, biotin, lipoic acid and folic acid; the inorganic salts comprise NaCl, KCl, MgCl2, CaCl2, Na2HPO4 and KH2PO4; and the trace elements comprise AlCl3, H3BO3, ZnSO4, MnCl2, CuSO4, FeSO4 and (NH4)6Mo7O24.4H2O. The synthetic medium is utilized for culturing the fissiparism chytrid based on a shake flask, and the output of the DHA is 9.8g/L.

The invention discloses schizochytrium limacinum named as Aurantiochytrium sp, and preserved in China General Microbiological Culture Collection Center (CGMCC) located at No. 3, courtyard 1, the western Beichen road, Chaoyang District of Beijing on Nov. 2nd, 2017, and the preservation number is CGMCC No:14849. The schizochytrium limacinum can be used for preparing DHA, docosapentaenoic acid greaseand squalene. The strain can resist high fermentation temperature, and the fermentation cost can be effectively lowered; the strain can rapidly grow under the condition that the temperature is 33-37DEG C, a large amount of polyunsaturated fatty acids (DHA and docosapentaenoic acid) and squalene grease can be accumulated, the growth speed is high, and the yield of polyunsaturated fatty acid grease is high.

The invention discloses a method for cultivating schizochytrium oil-producing grease employing schizochytrium and crude glycerine, and belongs to the technical field of microorganisms. The schizochytrium (Schizochytrium sp.) 691Y01 is preserved at the China General Microbiological Culture Collection Center; the accession number of the preservation center is CGMCC NO.9043; and the bacterial strain is obtained by ultraviolet mutagenesis of schizochytrium (Schizochytrium sp.) WZU6961. Compared with the Schizochytrium sp. WZU6961, the palmitic acid content is improved by 50% while the DHA is reduced by 30%; rapid growth and accumulation of grease are achieved by biodiesel byproduct crude glycerine as a carbon source; and grease components are relatively suitable for production of the biodiesel.

The invention relates to a new fragmentation chytrid variant. Especially, the invention relates to a fragmentation chytrid variant with improved DHA (docosahexaenoic acid) content and/or rapid growth speed and a method for producing DHA by utilizing the variant. Besides, the invention also relates to a method for producing the variant, comprising the steps of carrying out mutagenesis on fragmentation chytrid by adopting ultraviolet rays and screening a strain with rapid growth speed and high DHA content under selective pressure of an inhibitor (such as quizalofop) for fat synthesis key enzyme (acetyl coenzyme A carboxylase).

The invention provides a regulation and control method for improving the EPA (Eicosapentaenoic Acid) content in schizochytrium and application and relates to marine microorganisms. A strain schizochytrium sp. MYA1381 is subjected to flat plate and second-grade seed activation to prepare a seed solution; then the strain is inoculated to a fermentation culture medium; fluridone is added at differentphases of the growth of the schizochytrium. The fluridone can be applied to improvement of the EPA content in the schizochytrium. The fluridone is applied to regulation and control of a fatty acid synthesis way for the first time and a new thought is provided for improving the EPA content in the schizochytrium. The fluridone is added and the content of EPA in schizochytrium grease is remarkably improved and is improved by 42.31 percent when being compared with a manner without the fluridone. A foundation is provided for improving the EPA content in the schizochytrium by utilizing the fluridone.

The present invention relates to a fermenting method for enriching biomass of Thraustochytrium genus microalgae, specifically Schizochytrium sp. or Schizochytrium mangrovei, with docosahexaenoic acid (or DHA), by controlled addition of oxygen.

The invention relates to a method for producing DHA through fermentation and belongs to the field of microbial fermentation. The method comprises the following steps: using by-products obtained from maize processing as raw material, and adopting schizochytrium sp. for fermentation, so that the product DHA is obtained. In the whole fermentation process of the method, the adjustment and control of pH value are not required, the production cost is reduced, and the production efficiency is improved; by adopting the method provided by the invention, all components in the by-products obtained from maize processing can be used to the maximum extent, and the growth of microorganisms and product accumulation are facilitated. By adopting the method, the content of DHA in products prepared finally is increased obviously, the fatty acid in the DHA is changed obviously, the content of hexadecanoic acid is increased obviously and can reach 45.8%, an unexpected technical effect is achieved, meanwhile, the content of DHA in the products is increased obviously and can reach 47%, the oxidation stability of DHA grease is improved obviously, and the oxidation induction time is prolonged to 21.2 h from 1.1 h, and can reach 22.6 h to the maximum, so that the unexpected technical effect is produced.

The invention discloses a Schizochytrium sp. CGMCC No.5415 of a high yield of DHA (Docosahexaenoic Acid) as well as a fermentation method and an application of Schizochytrium sp. CGMCC No.5415. The biomass and the DHA can be rapidly accumulated by utilizing the Schizochytrium sp. CGMCC No.5415. Thus, the Schizochytrium sp. CGMCC No. 5415 has a wide industrial application value.

The invention discloses a method for producing docosahexaenoic acid by fermentation tank substrate feeding, and relates to docosahexaenoic acid. The method comprises the following steps: transferring a strain into a plate medium for cultivation to obtain an activated strain, wherein the strain is Schizochytrium sp. LU310, with the preservation number of CGMCC No. 12528; inoculating the obtained activated strain in a plate bacterial colony into a conical flask with a seed culture medium for cultivation; inoculating an obtained fermented culture solution into a fermentation culture medium for cultivation, automatically controlling the pH of the fermented solution by feeding 2M of NaOH or 1M of citric acid, sampling every 4 h to measure the glucose concentration, and sampling every 12 h to measure the biomass, the yield of grease and the yield of DHA.

The present invention discloses a microorganism DHA nutrition oil (docosahexaenoic acid) fermentation production method. According to the method, bacterial Schizochytrium sp. is inoculated into a shake flask to culture, then is inoculated into a seed tank to carry out scale-up culture, and finally is inoculated into a fermentation tank to carry out two-step fermentation, wherein in the first step, a temperature is controlled to 25-30 DEG C, the pH value is 6, a fermentation culture medium comprises glucose, yeast extract paste, peptone and an inorganic salt, and fermentation is performed for 2-3 days, in the second step, cooling is performed to a temperature of 20-25 DEG C, continuous fermentation is performed for 2-3 days, a concentration of the glucose is always controlled to 20% during the fermentation process by additionally supplying the glucose, the amount of microorganisms prepared through fermentation is about 60 g/L, oil content in dry biomass is 50-60%, DHA content is 35-40%, and DHA productivity is 8-10 g/L.day. The microorganism DHA fermentation production technology has characteristics of high DHA yield, high purity, stable production technology, simpleness and low cost.

The invention discloses application of catechin in improvement of DHA (docosahexaenoic acid) grease yield of schizochytrium limacinum, the grease yield is improved by culturing the schizochytrium limacinum with the catechin, and a small amount of catechin in a specific proportion is added to a schizochytrium limacinum fermentation system to inhibit generation of PEPC (polyepichlorohydrin) enzyme.Therefore, a metabolic network is disturbed to reduce biosynthesis of carbon flowing to lipid, the content of grease produced by schizochytrium limacinum fermentation can be effectively increased, theDHA yield is increased by 70% or above, the ratio of DHA to biomass is increased by 40% or above, the total grease is increased by 70% or above, and the beta carotene yield is also remarkably increased. Through simple fermentation regulation and control, the contents of DHA grease and beta carotene in the microbial schizochytrium limacinum can be remarkably increased, so that the production costis reduced, and the method has economic benefits.

The invention discloses a method for increasing added values of raw materials of schizochytrium sp. solid-state fermented feed. The method comprises the following steps: (1) fetching a schizochytrium sp. seed solution, uniformly mixing with a nutrient solution according to a ratio of 1:10-1:20 (v/v), and adjusting the pH to 6.0-7.0 to obtain mixed liquid; (2) fetching a solid substrate material, adding into the mixed liquid obtained in following step (1) according to a material-liquid ratio of 1:0.5-1:2 (w/v), uniformly mixing, standing and fermenting for 48-72h at 25-35 DEG C, wherein the solid substrate material is a mixture of starch and a protein. The invention further provides a fermented product prepared with the method and application thereof. With the method, the high-DHA content fermented product can be prepared; the method has simple process steps, low energy consumption, low cost and excellent industrial application prospect.

The invention relates to the technical field of microorganisms, in particular to a method for co-producing squalene and polyunsaturated fatty acid. According to the invention, schizochytrium limacinum separated from seawater is taken as an original strain, the schizochytrium limacinum capable of producing squalene and polyunsaturated fatty acid at the same time is obtained through mutagenesis, and the content of EPA (eicosapentaenoic acid) and the content of squalene in the product of the schizochytrium limacinum are at a relatively high level. According to the invention, the fermentation process is further optimized, and the pH value in the fermentation process is kept between 7 and 9; the temperature is 28-33 DEG C, the content of squalene is remarkably increased from 16.9% to 30% or above, and the method has important significance in the production field of squalene and polyunsaturated fatty acid.

The invention relates to microbial fermentation, in particular to a mixed fermentation method for schizochytrium sp. and crypthecodinium cohnii. The method includes the steps that the crypthecodinium cohnii is inoculated in a culture medium containing a carbon source, a nitrogen source and essential nutrient elements to be fermented and prepared into fermentation liquor; when the crypthecodinium cohnii enters in a logarithmic phase, the schizochytrium sp. is inoculated into the fermentation liquor in the step A for conducting mixed fermentation; the crypthecodinium cohnii belongs to the crypthecodinium cohnii algae species capable of accumulating polyunsaturated fatty acids, and belongs to the crypthecodinium cohnii genus; the schizochytrium sp. belongs to the schizochytrium sp. algae species capable of accumulating polyunsaturated fatty acids, and the mass percentage composition of palmitic acid in the schizochytrium sp. is not lower than 30%. By the adoption of the method, the problems that when existing crypthecodinium cohnii and schizochytrium sp. are fermented independently, the technical defects of varying degrees exist, follow-up treatment is complex and the production cost is high are solved, and the advantages that the technological period is short, the production cost is low, prepared products can meet the related standard requirement without complex follow-up treatment and the quality of fat is high are achieved.

The invention relates to a schizochytrium sp alpha-tubulin related sequence and application of the schizochytrium sp alpha-tubulin related sequence, in particular to a schizochytrium sp alpha-tubulin gene sequence as shown in SEQ ID No.1, a schizochytrium sp alpha-tubulin sequence as shown in SEQ ID No: 2 and a schizochytrium sp alpha-tubulin gene 3'end noncoding region sequence as shown in SEQ ID No: 3. The invention further relates to fragments of the gene sequences, recombinant nucleic acid molecules containing the gene sequences or fragments of the gene sequences, application of the recombinant nucleic acid molecules to homologous recombination, and a method for gene function analysis by using the recombinant nucleic acid molecules.

The present invention relates to Schizochytrium sp. with a high DHA content, application of the Schizochytrium sp. in preparation of DHA and a cultivation method of the Schizochytrium sp. The Schizochytrium sp. is preserved in China Center For Type Culture Collection on November 13, 2017, and the preservation number is CCTCCNO: M2017689. The Schizochytrium sp. W1 grows fast and can undergo complete heterotrophic growth. Under the condition of nitrogen deficiency induction, the Schizochytrium sp. can accumulate a large amount of the DHA. Therefore, the Schizochytrium sp. and the culture methodcan be used to prepare the DHA or food additives or feed additives with high DHA contents, and have good economic benefits.