5 results about "Schizochytrium sp." patented technology

This invention discloses a fermentation method for increasing the yield of odd-chain fatty acids from *Schizochytrium*. *Schizochytrium* is inoculated into a fermentation medium containing assimilated carbon and nitrogen sources for fermentation. The carbon sources include carbon source a (a common carbon source) and carbon source b (a precursor that can be converted into propionyl-CoA intracellularly). The fermentation is carried out in a shake flask or fermenter. When fermentation is carried out in a fermenter, carbon source b is continuously added to the fermentation system at a constant flow rate. This invention, by establishing a mixed carbon source culture system and continuously supplementing propionyl-CoA precursors during fermentation in a fermenter, reduces the transient toxicity of propionic acid, maintains cellular energy metabolism and redox homeostasis, and improves the effective supply of intracellular propionyl-CoA, thereby significantly increasing the yield of odd-chain fatty acids.

This invention relates to a method for preparing and applying a fermented product of Bifida Ferment Lysate and Dioscorea Oolong with enhanced moisturizing and anti-aging effects, belonging to the field of microbial fermentation technology. This invention discloses a *Bifidobacterium longum*, the nucleotide sequence of which is shown in SEQ ID NO: 1. This invention utilizes *Bifidobacterium longum* HJ-005 to ferment *Dioscorea opposita* and *Dioscorea opposita* to prepare a *Bifida Ferment Lysate* fermented product of *Dioscorea opposita*, simultaneously increasing the content of absorbable diosgenin, anthocyanins, arginine, and organic selenium, with better effects compared to other lactic acid bacteria. It can significantly reduce the water loss and shrinkage of zebrafish embryos, exhibiting a moisturizing effect, and can significantly promote the expression of zebrafish type I collagen and Elna genes, promoting collagen and elastin regeneration, thus possessing anti-aging effects and can be used as a new raw material for cosmetics.

Disclosed are a ruminant methane emission inhibitor, a preparation method thereof, and use thereof. The ruminant methane emission inhibitor of the present application is prepared from components including 1-5 parts by weight of Schizochytrium sp. powder and 0.05-0.5 parts by weight of cellulase. The ruminant methane emission inhibitor of the present application can reduce the amount of methane generated by ruminants while increasing the total fermentation activity of the rumen.

The application belongs to the technical field of bioengineering, and discloses a genetically engineered strain for DHA oil production of Schizochytrium sp. based on carbon flux guidance and enhanced fatty acid transport, a method and application thereof. The genetically engineered strain is obtained by introducing optimized coding genes ACLY and / or Fat1p coding genes related to carbon flux guidance and fatty acid transport into Schizochytrium sp. in vivo. The gene sequence of the gene ACLY is SEQ ID No. 9, and the gene sequence of the gene Fat1p is SEQ ID No. 10. Through metabolic engineering, the application constructs a Schizochytrium sp. engineering strain for DHA oil production based on carbon flux guidance and enhanced fatty acid transport. The Schizochytrium sp. engineering strain is based on the combination of carbon flux guidance and fatty acid transport, which can not only be used for oil synthesis by guiding carbon flux, but also avoid oil degradation by fatty acid transport.

This invention belongs to the fields of genetic engineering and fermentation engineering, and discloses a method for constructing and applying a Schizochytrium strain that produces high DHA content. The engineered Schizochytrium strain uses Schizochytrium sp. HX308 as the starting strain. Through metabolic engineering, a complete elongation-desaturation pathway is established in Schizochytrium (overexpression of C16 / 18 elongase, Δ-9 elongase gene, Δ-5 desaturase gene, Δ-17 desaturase gene, Δ-5 elongase gene, and Δ-4 desaturase gene), which works synergistically with the PKS pathway to produce DHA. The peroxisome synthesis factor PEX10 is knocked out to obtain the recombinant engineered strain SCD3. In fed-batch fermentation, the final DHA content of the engineered Schizochytrium strain SCD3 is the highest to date, reaching 70.1% of the total fatty acid content. The oil and DHA yields are also significantly increased, reaching 67.9 g / L and 47.6 g / L, respectively. The DHA yield is 1.7 times that of the wild type. This engineered strain has good application prospects.