63 results about "Schizochytrium sp." patented technology

The present invention relates to the Schizochytrium genus SR21 strain and a microorganism belonging to the same species as does said SR21 strain or having substantially the same fungological properties as does said SR21 strain, the said SR21 strain and microorganism having the ability to produce the (n-3) series of docosahexaenoic acid (DHA) and the (n-6) series of docosapentaenoic acid (DPA), and the invention also relates to a process for preparing the (n-3) series of DHA and the (n-6) series of DPA utilizing said microorganisms. The microorganisms according to the present invention are superior in their proliferation character and their propensity to produce fat, and have the ability to produce the (n-3) series of DHA and the (n-6) series of DPA very well. Accordingly, it is possible to effectively produce the (n-3) series of DHA and / or the (n-6) series of DPA, which are useful in the fields of foods and pharmaceuticals, using the microorganisms according to the present invention. In addition, the present invention provides a fat obtained by culturing the present microorganisms. Since the fat composition contains the (n-6) series of DPA in addition to the (n-3) series of DHA having various physiological activities, it is possible to stably and effectively supply the (n-6) series of DPA and / or the (n-3) series of DHA to subjects in need of these highly unsaturated fatty acids by adding the fat composition to various feedstuffs or foods.

The invention discloses a Schizochytrium sp., which has a category name of Schizochytrium sp., is preserved in Common Microorganisms Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CCTCC No.209059. The invention also discloses a method for producing DHA lipa by using the Schizochytrium sp. The method optimizes a strain fermentation medium from the perspectives of osmotic pressure and element supply and combines a fed-batch strategy to achieve high-density fermentation of microalgae, thus the final dry cell weight reaches 70 grams per liter, the lipa content reaches 31.5 grams per liter, and the DHA accounts for more than 35 percent of the total fatty acid content. All indexes of the DHA essential oil obtained by the method accord with the standard of food additives, and the DHA essential oil contains a bioactive substance of squalene. The method has the advantages of simple entire process, convenient operation, and high biomass and DHA content, reduces the fermentation cost, and is suitable for industrial production.

The present disclosure provides a novel mutant strain of Schizochytrium limacinum having the Accession No. MTCC 5249, which produces lipids and extracellular polysaccharide (EPS) simultaneously. The disclosure further provides a process for simultaneous production of lipids and extracellular polysaccharide (EPS) from the novel mutant strain of Schizochytrium limacinum. The lipids produced from the novel mutant strain of Schizochytrium limacinum comprises docosahexaenoic acid (DHA). The disclosure also provides a food, feed, cosmetic, nutritional or therapeutic supplement for humans or animals comprising the cell biomass and extracellular polysaccharides (EPS) of the mutant strain of Schizochytrium limacinum. A cosmetic composition comprising the extracellular polysaccharides (EPS) of Schizochytrium limacinum is also provided that is useful as a base for cosmetics for topical application. The present disclosure further provides a pickle composition and a fat product having improved nutritive value.

The invention discloses a Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and a fermentation method thereof. The Schizochytrium sp.TIO1101 strain provided by the invention has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.4603. After the Schizochytrium sp.TIO1101 is subjected to high-density heterotrophic fermentation for 72h, the biomass liveweight, the lipid content and the DHA are respectively 120g / L, 54.3% and 51.4% of the content of total lipid, therefore the Schizochytrium sp.TIO1101 has development and application values extremely. The Schizochytrium sp.TIO1101 disclosed by the invention has the advantages that: the Schizochytrium sp.TIO1101is obtained through separation; the high-density heterotrophic fermentation of the Schizochytrium sp.TIO1101 is realized; and the lipid extracted from the Schizochytrium sp.TIO1101 cells obtained under the fermentation condition has high DHA content and purer components.

The invention relates to a preparation method of DHA (which relates to an unsaturated fatty acid. The invention provides a preparation method of a fully synthetic medium that is suitable for high density growth of fissiparism chytrid and increases the output of the DHA. The DHA comprises a carbon source, amino acids, lysine, inorganic salts, vitamins, trace elements and water. The carbon source is glucose, the amino acids comprise alanine, methionine, cysteine, lysine, histidine, glutamic acid, glutamine, isoleucine, threonine and tryptophan; the vitamins comprise VB1, VB12, biotin, lipoic acid and folic acid; the inorganic salts comprise NaCl, KCl, MgCl2, CaCl2, Na2HPO4 and KH2PO4; and the trace elements comprise AlCl3, H3BO3, ZnSO4, MnCl2, CuSO4, FeSO4 and (NH4)6Mo7O24.4H2O. The synthetic medium is utilized for culturing the fissiparism chytrid based on a shake flask, and the output of the DHA is 9.8g / L.

The invention relates to a fermentation production method of docosahexenoic acid (DHA), in particular to a liquid fermentation production method of docosahexenoic acid (DHA) through solid material cultivation of the schizochytrium sp. KDW-12. An applied bacterium strain is the schizochytrium sp. KDW-12. The liquid fermentation production method of the DHA includes the following steps of activation of the bacterium strain, preparation of bacterium suspension, preparation of one-level seed, preparation of two-level solid material seed, enlarging cultivation of the two-level solid material seed in a fermentation tank, collection of thallus cells after fermentation, and extraction of DHA oil. The extracted DHA can be used for a nutrient supplement and provides long-chain polyunsaturated fatty acid needed by human and animals. A seed preparation method is improved, the seed enlarging technology is simplified, a seed culture and fermentation period is shortened, and therefore investment is reduced and cost is saved. In addition, a solid material culture medium is full of nutriments and is rich in a plurality of growth factors and more beneficial to growth of the seed. Bacterium strain cells are strong in viability and good in synchronism. Besides, waste water, waste residue and environmental pollution which are generated in the stage of solid material culture are less.

The invention discloses schizochytrium limacinum named as Aurantiochytrium sp, and preserved in China General Microbiological Culture Collection Center (CGMCC) located at No. 3, courtyard 1, the western Beichen road, Chaoyang District of Beijing on Nov. 2nd, 2017, and the preservation number is CGMCC No:14849. The schizochytrium limacinum can be used for preparing DHA, docosapentaenoic acid greaseand squalene. The strain can resist high fermentation temperature, and the fermentation cost can be effectively lowered; the strain can rapidly grow under the condition that the temperature is 33-37DEG C, a large amount of polyunsaturated fatty acids (DHA and docosapentaenoic acid) and squalene grease can be accumulated, the growth speed is high, and the yield of polyunsaturated fatty acid grease is high.

The invention discloses a bacterium strain for producing docosahexaenoic acid and a use thereof. The bacterium strain for producing docosahexaenoic acid is Schizoochytrium limacinum HS01, is preserved in the China general microbiological culture collection center and has the accession number of CGMCC No. 13746. The experiment results show that Schizoochytrium limacinum HS01 is subjected to fermentation culture so that fermentation broth is obtained, and the fermentation broth has DHA content of 45.0% to 60.0% in oil and thus the bacterium strain has an important application value.

The invention discloses Schizochytrium sp and method for producing docosahexenoic acid grease. The Schizochytrium sp S056 is preserved in CCTCC in September 29, 2013, and the preservation number is CCTCC M2013459. The Schizochytrium sp S056 with stable and high yield can be obtained by a method combined with mutagenesis breeding and rapid screening, and dissolved oxygen detection supplementary material and temperature control technology is used, and glucose concentration of fermentation medium is regulated and controlled for completely using glucose, and DHA yield of the bacterial strain can reach 23 g / L at the highest, and the productivity can reach 8.2g / L.d at the highest, and the DHA has a high product quality and the DHA content can reach 51%. The bacterial strain can produce DHA, and the fermentation condition is simple and easy to control, and is suitable for growth under the low salinity condition without seawater or seawater crystals with short fermentation period and high production efficiency, and the fermentation waste water is easy to be treated, and the product is suitable for industrial application.

The invention discloses a method for cultivating schizochytrium oil-producing grease employing schizochytrium and crude glycerine, and belongs to the technical field of microorganisms. The schizochytrium (Schizochytrium sp.) 691Y01 is preserved at the China General Microbiological Culture Collection Center; the accession number of the preservation center is CGMCC NO.9043; and the bacterial strain is obtained by ultraviolet mutagenesis of schizochytrium (Schizochytrium sp.) WZU6961. Compared with the Schizochytrium sp. WZU6961, the palmitic acid content is improved by 50% while the DHA is reduced by 30%; rapid growth and accumulation of grease are achieved by biodiesel byproduct crude glycerine as a carbon source; and grease components are relatively suitable for production of the biodiesel.

The invention relates to a new fragmentation chytrid variant. Especially, the invention relates to a fragmentation chytrid variant with improved DHA (docosahexaenoic acid) content and / or rapid growth speed and a method for producing DHA by utilizing the variant. Besides, the invention also relates to a method for producing the variant, comprising the steps of carrying out mutagenesis on fragmentation chytrid by adopting ultraviolet rays and screening a strain with rapid growth speed and high DHA content under selective pressure of an inhibitor (such as quizalofop) for fat synthesis key enzyme (acetyl coenzyme A carboxylase).

The invention provides a regulation and control method for improving the EPA (Eicosapentaenoic Acid) content in schizochytrium and application and relates to marine microorganisms. A strain schizochytrium sp. MYA1381 is subjected to flat plate and second-grade seed activation to prepare a seed solution; then the strain is inoculated to a fermentation culture medium; fluridone is added at differentphases of the growth of the schizochytrium. The fluridone can be applied to improvement of the EPA content in the schizochytrium. The fluridone is applied to regulation and control of a fatty acid synthesis way for the first time and a new thought is provided for improving the EPA content in the schizochytrium. The fluridone is added and the content of EPA in schizochytrium grease is remarkably improved and is improved by 42.31 percent when being compared with a manner without the fluridone. A foundation is provided for improving the EPA content in the schizochytrium by utilizing the fluridone.

The present invention relates to a fermenting method for enriching biomass of Thraustochytrium genus microalgae, specifically Schizochytrium sp. or Schizochytrium mangrovei, with docosahexaenoic acid (or DHA), by controlled addition of oxygen.

The invention relates to a method for producing DHA through fermentation and belongs to the field of microbial fermentation. The method comprises the following steps: using by-products obtained from maize processing as raw material, and adopting schizochytrium sp. for fermentation, so that the product DHA is obtained. In the whole fermentation process of the method, the adjustment and control of pH value are not required, the production cost is reduced, and the production efficiency is improved; by adopting the method provided by the invention, all components in the by-products obtained from maize processing can be used to the maximum extent, and the growth of microorganisms and product accumulation are facilitated. By adopting the method, the content of DHA in products prepared finally is increased obviously, the fatty acid in the DHA is changed obviously, the content of hexadecanoic acid is increased obviously and can reach 45.8%, an unexpected technical effect is achieved, meanwhile, the content of DHA in the products is increased obviously and can reach 47%, the oxidation stability of DHA grease is improved obviously, and the oxidation induction time is prolonged to 21.2 h from 1.1 h, and can reach 22.6 h to the maximum, so that the unexpected technical effect is produced.

The invention discloses a Schizochytrium sp. CGMCC No.5415 of a high yield of DHA (Docosahexaenoic Acid) as well as a fermentation method and an application of Schizochytrium sp. CGMCC No.5415. The biomass and the DHA can be rapidly accumulated by utilizing the Schizochytrium sp. CGMCC No.5415. Thus, the Schizochytrium sp. CGMCC No. 5415 has a wide industrial application value.

The invention discloses a Schizochytrium sp., which has a category name of Schizochytrium sp., is preserved in Common Microorganisms Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CCTCC No.209059. The invention also discloses a method for producing DHA lipa by using the Schizochytrium sp. The method optimizes a strain fermentation medium from the perspectives of osmotic pressure and element supply and combines a fed-batch strategy to achieve high-density fermentation of microalgae, thus the final dry cell weight reaches 70 grams per liter, the lipa content reaches 31.5 grams per liter, and the DHA accounts for more than 35 percent of the total fatty acid content. All indexes of the DHA essential oil obtained by the method accord with thestandard of food additives, and the DHA essential oil contains a bioactive substance of squalene. The method has the advantages of simple entire process, convenient operation, and high biomass and DHA content, reduces the fermentation cost, and is suitable for industrial production.

The invention discloses a method for producing docosahexaenoic acid by fermentation tank substrate feeding, and relates to docosahexaenoic acid. The method comprises the following steps: transferring a strain into a plate medium for cultivation to obtain an activated strain, wherein the strain is Schizochytrium sp. LU310, with the preservation number of CGMCC No. 12528; inoculating the obtained activated strain in a plate bacterial colony into a conical flask with a seed culture medium for cultivation; inoculating an obtained fermented culture solution into a fermentation culture medium for cultivation, automatically controlling the pH of the fermented solution by feeding 2M of NaOH or 1M of citric acid, sampling every 4 h to measure the glucose concentration, and sampling every 12 h to measure the biomass, the yield of grease and the yield of DHA.

The present invention discloses a microorganism DHA nutrition oil (docosahexaenoic acid) fermentation production method. According to the method, bacterial Schizochytrium sp. is inoculated into a shake flask to culture, then is inoculated into a seed tank to carry out scale-up culture, and finally is inoculated into a fermentation tank to carry out two-step fermentation, wherein in the first step, a temperature is controlled to 25-30 DEG C, the pH value is 6, a fermentation culture medium comprises glucose, yeast extract paste, peptone and an inorganic salt, and fermentation is performed for 2-3 days, in the second step, cooling is performed to a temperature of 20-25 DEG C, continuous fermentation is performed for 2-3 days, a concentration of the glucose is always controlled to 20% during the fermentation process by additionally supplying the glucose, the amount of microorganisms prepared through fermentation is about 60 g / L, oil content in dry biomass is 50-60%, DHA content is 35-40%, and DHA productivity is 8-10 g / L.day. The microorganism DHA fermentation production technology has characteristics of high DHA yield, high purity, stable production technology, simpleness and low cost.

The invention relates to the field of microbial oils, in particular to a method for producing a composite polyunsaturated fatty acid oil by using schizochytrium sp.. The method comprises the steps that the schizochytrium sp. is subjected to fermentation culture; bacteria after fermentation culture are resuspended with water, and cellulase and neutral protease are added for enzymolysis; an enzymatic hydrolyzate is mixed with n-hexane, shaking extraction and centrifugation are conducted, an n-hexane phase is collected, concentration under reduced pressure is conducted to remove the n-hexane, anddrying is conducted to obtain an oil; the oil is subjected to first-stage crystallization by means of a freezing crystallization technique, the liquid oil is subjected to second-stage crystallization, and cold filtration is conducted to obtain the liquid oil after secondary crystallization; the liquid oil is deacidified and decolorized. The method has the advantages that by regulating fermentation conditions and conducting concentration processing on polyunsaturated fatty acids, the content of eicosapentaenoic acid in the schizochytrium sp. is successfully increased to 12% or above, and the produced oil is rich in docosahexaenoic acid, docosapentaenoic acid and eicosapentaenoic acid and is a novel oil product capable of replacing the polyunsaturated fatty acids from fish oils.

The invention belongs to the technical field of bioengineering, and discloses a method for producing EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) by fermentation of schizochytrium limacinum, which comprises the following steps: by taking schizochytrium limacinum as a production strain, adding ensidipine into a fermentation culture medium, and fermenting to produce polyunsaturated fatty acid. According to the method, the EPA content and the DHA content in a schizochytrium limacinum fermentation product are increased by activating a part of the ELO / DES pathway of the schizochytrium limacinum, and the EPA content in the fermentation product is increased by 8.73 times and the DHA content is increased by 1.15 times by adopting the schizochytrium limacinum engineering bacterium EDG as a production strain.

The invention discloses a Schizochytrium sp.TIO1101 strain with high-yield DHA (docosahexaenoic acid) and a fermentation method thereof. The Schizochytrium sp.TIO1101 strain provided by the invention has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.4603. After the Schizochytrium sp.TIO1101 is subjected to high-density heterotrophic fermentation for 72h, the biomass liveweight, the lipid content and the DHA are respectively 120g / L, 54.3% and 51.4% of the content of total lipid, therefore the Schizochytrium sp.TIO1101 has development and application values extremely. The Schizochytrium sp.TIO1101 disclosed by the invention has the advantages that: the Schizochytrium sp.TIO1101is obtained through separation; the high-density heterotrophic fermentation of the Schizochytrium sp.TIO1101 is realized; and the lipid extracted from the Schizochytrium sp.TIO1101 cells obtained under the fermentation condition has high DHA content and purer components.

Disclosed is a process for growing the microflora Thraustochytrium, Schizochytrium, and mixtures thereof, which includes the growing of the microflora in fermentation medium containing non-chloride containing sodium salts, in particular sodium sulfate. In a preferred embodiment of the present invention, the process produces microfloravhaving a cell aggregate size useful for the production of food products for use in aquaculture. Further disclosed is a food product which includes Thraustochytrium, Schizochytrium, and mixtures thereof, and a component selected from flaxseed, rapeseed, soybean and avocado meal. Such a food product includes a balance of long chain and short chain omega-3 highly unsaturated fatty acids.

The invention discloses a method for preparing docosahexenoic acid from schizochytrium sp. The method comprises the following steps: (1) culturing a schizochytrium sp. strain capable of growing in a virus-infected bagasse hydrolysate directly, so as to obtain a seed fluid; (2) inoculating the seed fluid into a fermentation culture fluid prepared from the virus-infected bagasse hydrolysate for fermentation culture, so as to obtain docosahexenoic acid. The method is low in cost, easy to control, high in schizochytrium sp. yield and DHA content.

The invention discloses application of catechin in improvement of DHA (docosahexaenoic acid) grease yield of schizochytrium limacinum, the grease yield is improved by culturing the schizochytrium limacinum with the catechin, and a small amount of catechin in a specific proportion is added to a schizochytrium limacinum fermentation system to inhibit generation of PEPC (polyepichlorohydrin) enzyme.Therefore, a metabolic network is disturbed to reduce biosynthesis of carbon flowing to lipid, the content of grease produced by schizochytrium limacinum fermentation can be effectively increased, theDHA yield is increased by 70% or above, the ratio of DHA to biomass is increased by 40% or above, the total grease is increased by 70% or above, and the beta carotene yield is also remarkably increased. Through simple fermentation regulation and control, the contents of DHA grease and beta carotene in the microbial schizochytrium limacinum can be remarkably increased, so that the production costis reduced, and the method has economic benefits.

The invention discloses a method for increasing added values of raw materials of schizochytrium sp. solid-state fermented feed. The method comprises the following steps: (1) fetching a schizochytrium sp. seed solution, uniformly mixing with a nutrient solution according to a ratio of 1:10-1:20 (v / v), and adjusting the pH to 6.0-7.0 to obtain mixed liquid; (2) fetching a solid substrate material, adding into the mixed liquid obtained in following step (1) according to a material-liquid ratio of 1:0.5-1:2 (w / v), uniformly mixing, standing and fermenting for 48-72h at 25-35 DEG C, wherein the solid substrate material is a mixture of starch and a protein. The invention further provides a fermented product prepared with the method and application thereof. With the method, the high-DHA content fermented product can be prepared; the method has simple process steps, low energy consumption, low cost and excellent industrial application prospect.

The invention discloses a culture medium added with a surfactant and application thereof. The culture medium added with the surfactant is composed of glycerinum or glucose which is taken as a major carbon source, a yeast extract and / or sodium glutamate which are / is taken as a major nitrogen source, inorganic salt components comprising Na2SO4, KH2PO4, K2HPO4, K2SO4 and MgSO4.7H2O, vitamins VB1, VB6 and VB12, and Tween 20, Tween 40, Tween 60 or Tween 60 added separately, wherein the concentration of the glycerinum or the glucose is 10-120g / L, and the concentration of the yeast extract and / or the sodium glutamate is 5-50g / L. The surfactant of an appropriate concentration is added to the culture medium so that the cell membrane permeability of microorganisms is changed, mass transfer of a substrate and oxygen is strengthened and growth is promoted; due to the adopted technical scheme, the conversion rate of the substrate can be increased without extra labor or equipment investment, and therefore, the fermentation of schizochytrium limacinum is effectively reduced.

The invention relates to the technical field of microorganisms, in particular to a method for co-producing squalene and polyunsaturated fatty acid. According to the invention, schizochytrium limacinum separated from seawater is taken as an original strain, the schizochytrium limacinum capable of producing squalene and polyunsaturated fatty acid at the same time is obtained through mutagenesis, and the content of EPA (eicosapentaenoic acid) and the content of squalene in the product of the schizochytrium limacinum are at a relatively high level. According to the invention, the fermentation process is further optimized, and the pH value in the fermentation process is kept between 7 and 9; the temperature is 28-33 DEG C, the content of squalene is remarkably increased from 16.9% to 30% or above, and the method has important significance in the production field of squalene and polyunsaturated fatty acid.

The invention discloses a preparation method of an egg yolk dairy product rich in astaxanthin and DHA (docosahexaenoic acid). The method comprises the following steps: D1, taking haematococcus pluvialis as a basic source of astaxanthin, and obtaining eggs of which each yolk contains 6mg of astaxanthin; D2, taking schizochytrium limacinum as a basic source of DHA (docosahexaenoic acid), and obtaining eggs of which each yolk contains 200mg of DHA; D3, separating egg white and egg yolk of the eggs, and performing pasteurization on the egg yolk for later use; D4, pasteurizing milk for later use; D5, putting the astaxanthin yolk, the DHA yolk, the milk and the flavoring agent into a stirrer, fully stirring, passing through a high-pressure homogenizer, and filtering for later use; and D6, performing low-temperature pasteurization or instant high-temperature sterilization on the egg-milk mixed liquid which is subjected to high-pressure homogenization and mixing, and filling a commodity container with the egg-milk mixed liquid. Through low-temperature pasteurization of the egg liquid and secondary low-temperature pasteurization and instantaneous high-temperature sterilization of the egg-milk mixed liquid, the safety and commodity circulation of the egg-milk finished product are ensured.

The invention relates to microbial fermentation, in particular to a mixed fermentation method for schizochytrium sp. and crypthecodinium cohnii. The method includes the steps that the crypthecodinium cohnii is inoculated in a culture medium containing a carbon source, a nitrogen source and essential nutrient elements to be fermented and prepared into fermentation liquor; when the crypthecodinium cohnii enters in a logarithmic phase, the schizochytrium sp. is inoculated into the fermentation liquor in the step A for conducting mixed fermentation; the crypthecodinium cohnii belongs to the crypthecodinium cohnii algae species capable of accumulating polyunsaturated fatty acids, and belongs to the crypthecodinium cohnii genus; the schizochytrium sp. belongs to the schizochytrium sp. algae species capable of accumulating polyunsaturated fatty acids, and the mass percentage composition of palmitic acid in the schizochytrium sp. is not lower than 30%. By the adoption of the method, the problems that when existing crypthecodinium cohnii and schizochytrium sp. are fermented independently, the technical defects of varying degrees exist, follow-up treatment is complex and the production cost is high are solved, and the advantages that the technological period is short, the production cost is low, prepared products can meet the related standard requirement without complex follow-up treatment and the quality of fat is high are achieved.