30 results about "Schizochytrium sp." patented technology

The invention relates to the technical field of microbial fermentation, and discloses application of a regulatory factor in promoting schizochytrium limacinum to produce DHA (docosahexaenoic acid), and the regulatory factor contains beta-carotene and reduced glutathione. In addition, the invention also provides a method for improving DHA production of schizochytrium limacinum and a preparation method of microbial oil. The method comprises the following steps: fermenting schizochytrium limacinum in a culture medium, and adding beta-carotene and reduced glutathione into the culture medium in the fermentation process; in the culture medium, the content of the beta-carotene is 1.5 to 2g / L. The beta-carotene and the reduced glutathione can effectively improve the content of grease and DHA prepared by fermentation of schizochytrium limacinum, the content of DHA can reach 63.9%, and the beta-carotene and the reduced glutathione are non-toxic and high in safety.

The invention belongs to the technical field of biology, and discloses a yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application of the yarrowia lipolytica engineering strain. Delta9 elongase, Delta8 desaturase, Delta5 desaturase and Delta17 desaturase genes in a fatty acid synthesis route, heterologous ATP-citrate lyase and a mitochondrial citric acid vector are overexpressed. According to the invention, ATP-citric acid lyase and a mitochondrial citric acid carrier gene which are respectively derived from Schizochylene sp. And Saccharomyces cerevisiae are jointly expressed in the yarrowia lipolytica engineering strain, so that the yields of EPA and grease in the yarrowia lipolytica engineering strain are remarkably improved, and compared with a control strain, the yields of the grease and the EPA are respectively improved by 243.35% and 161.85%.

The invention relates to the technical field of genetic engineering, in particular to a recombinant vector, an expression plasmid, a recombinant schizochytrium limacinum mutant and a method for increasing the DHA content of microorganisms. The LIP1 gene for coding lipase in the microbial lipid droplet protein is knocked out through a homologous recombination technology, and the production of lipase in the microbial lipid droplet can be effectively inhibited, so that the hydrolysis process of triglyceride of polyunsaturated fatty acid stored in the lipid droplet to glycerol and free fatty acid in the later stage of microbial culture is blocked, and DHA is prevented from being lost from a TAG repository; experimental verification shows that in microorganisms represented by schizochytrium limacinum, the DHA content can be increased by 21.8% compared with that of a wild type, the yield and purity of DHA are increased, the stability of the microbial fermentation process is guaranteed, and application and popularization are facilitated.

The invention belongs to the technical field of biochemical engineering, and discloses a production method for improving the content of odd-chain fatty acid in grease by modifying schizochytrium limacinum and optimizing a substrate and application of the method. According to the method, schizochytrium limacinum HX-308 serves as a receptor strain, high-yield odd-chain fatty acid engineering bacteria are obtained through gene overexpression and inhibition, and the high-yield odd-chain fatty acid is obtained. Accumulation of a propionyl CoA precursor pool for synthesizing odd-chain fatty acid is improved, endogenous methylmalonyl-CoA mutase coding gene MUT is inhibited, so that a competitive pathway of the odd-chain fatty acid is inhibited, and then through substrate optimization, the content of fermented grease and the content of the odd-chain fatty acid are obviously improved. The expression of related enzymes in schizochytrium limacinum is adjusted through a genetic engineering strategy to improve the odd-chain fatty acid synthesis, the synthesis and utilization of the key intermediate propionyl coenzyme A in the odd-chain fatty acid synthesis process are increased, the metabolic pathway of the propionyl coenzyme A is inhibited, and substrate optimization is assisted to improve the odd-chain fatty acid yield.

The invention belongs to the technical field of bioengineering, and discloses a genetic engineering strain for enhancing schizochytrium limacinum DHA oil production based on carbon flux guidance and fatty acid transport, a method and application thereof. The gene engineering strain is obtained by introducing an optimized coding gene ACLY and / or Fat1p coding gene related to guiding carbon flux and fatty acid transport into a schizochytrium limacinum body; wherein the gene sequence of the gene ACLY is SEQ ID No. 9, and the gene sequence of the gene Fat1p is SEQ ID No. 10. According to the invention, a schizochytrium limacinum engineering bacterium for enhancing DHA oil production based on carbon flux guidance and fatty acid transfer is constructed by means of metabolic engineering. The schizochytrium limacinum engineering bacterium is combined with fatty acid transfer based on carbon flux guiding, can be used for grease synthesis by guiding carbon flux, and can prevent grease from being degraded through fatty acid transfer.

The invention provides a method for producing polyunsaturated fatty acid, and belongs to the technical field of biological fermentation. The method for producing polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid from schizochytrium limacinum comprises the following steps: activating schizochytrium limacinum in a seed culture medium to obtain a schizochytrium limacinum seed culture solution; inoculating the schizochytrium limacinum seed culture solution into a fermentation culture medium for culturing, wherein the fermentation culture medium comprises rapeseed protein powder; and extracting the culture solution in the cultured fermentation culture medium to obtain the polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid. According to the present invention, the yield of polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid can be significantly improved.

The invention relates to the technical field of grease processing, and discloses a method for increasing the sn-2 DHA content in schizochytrium limacinum triglyceride. The method comprises the following steps: adding a metabolic regulator into a fermentation culture solution containing schizochytrium limacinum, carrying out fermentation culture until the total fermentation time is more than 120 hours, and collecting a grease product, the metabolic regulator is selected from at least one of choline chloride, inositol and lysophosphatidic acid. According to the method provided by the invention, the lipid metabolic flux of schizochytrium limacinum can be effectively regulated and controlled, and directional enrichment of DHA to the sn-2 site of triglyceride is remarkably promoted, so that the yield and content of sn-2 DHA are improved.

The invention belongs to the technical field of biochemical engineering, and discloses a method for promoting schizochytrium limacinum to quickly accumulate Omega-3 fatty acid by using a natural antioxidant and application of the schizochytrium limacinum, the method comprises the following steps: performing fermentation culture on the schizochytrium limacinum, adding a mixed exogenous antioxidant melatonin and a rosemary extract in the fermentation culture process, and performing fermentation culture on the mixed exogenous antioxidant melatonin and the rosemary extract to obtain the Omega-3 fatty acid-containing schizochytrium limacinum. The melatonin and rosemary extract mixed antioxidant can interact to regulate fermentation in schizochytrium limacinum to increase fatty acid synthesis and oxidation stability. According to the present invention, in the experiment of schizochytrium limacinum fermentation oil production, the addition of the specific amount of the mixed antioxidant composition during the fermentation results show that the oil yield can be increased, the PUFA ratio in the specific concentration range can be significantly increased by 32%, the PUFA use amount can be reduced by 40% through the compounding use, and the method can save manpower, material resources and cost, and can provide the good application prospect. The grease production efficiency is greatly improved, and the method is suitable for industrial production.

This invention discloses a fermentation method for increasing the yield of odd-chain fatty acids from *Schizochytrium*. *Schizochytrium* is inoculated into a fermentation medium containing assimilated carbon and nitrogen sources for fermentation. The carbon sources include carbon source a (a common carbon source) and carbon source b (a precursor that can be converted into propionyl-CoA intracellularly). The fermentation is carried out in a shake flask or fermenter. When fermentation is carried out in a fermenter, carbon source b is continuously added to the fermentation system at a constant flow rate. This invention, by establishing a mixed carbon source culture system and continuously supplementing propionyl-CoA precursors during fermentation in a fermenter, reduces the transient toxicity of propionic acid, maintains cellular energy metabolism and redox homeostasis, and improves the effective supply of intracellular propionyl-CoA, thereby significantly increasing the yield of odd-chain fatty acids.

Provided herein are microorganism of a Thraustochytrid and a method for preparing bio-oil using the same, and more particularly, Schizochytrium sp. PB31 (PTA-123692) having bio-oil producibility, and a method of preparing bio-oil, particularly bio-oil having a content of omega-3 and / or 6 long-chain polyunsaturated fatty acids of 30% or more by weight or more based on total fatty acids, characterized by culturing the microorganism.

The application belongs to the technical field of biology, and discloses a Yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application, wherein a chassis strain of the engineering strain is Yarrowia lipolytica Po1f, and genes of Δ9 elongase, Δ8 desaturase, Δ5 desaturase and Δ17 desaturase in a fatty acid synthesis pathway, a heterologous ATP-citrate lyase and a mitochondrial citrate carrier are overexpressed. ATP-citrate lyases and mitochondrial citrate carrier genes respectively derived from Schizochytrium sp. and Saccharomyces cerevisiae are combined to be expressed in the Yarrowia lipolytica engineering strain, so that the production of EPA and oil in the Yarrowia lipolytica engineering strain is significantly improved, and compared with a control strain, the production of oil and EPA is respectively increased by 243.35% and 161.85%.

The invention belongs to the technical field of biochemical engineering, and discloses a method for producing squalene by inducing schizochytrium limacinum through a salt stress strategy and application, the method comprises the following steps: fermenting a schizochytrium limacinum strain for fermentation for 0-72 hours, adding 3g / L of NaCl into a fermentation medium, after 72 hours, adding NaCl into the fermentation liquid to 5g / L, continuing to ferment for 120 hours, measuring the sugar consumption every 12 hours in the fermentation process, and supplementing glucose to 80g / L. According to the method, the squalene yield of schizochytrium limacinum is remarkably increased through a salt stress strategy, an expensive inducer is prevented from being used, and the production cost is effectively reduced. 3 g / L of low-concentration salt is added in 0-72 h of the early stage of fermentation to serve as an adaptation period, so that schizochytrium limacinum grows rapidly and is induced to start an osmotic regulation mechanism, the concentration of NaCl is increased to 5 g / L in 72 h, at the moment, the schizochytrium limacinum enters a lipid accumulation period, and high-concentration NaCl is utilized to stress the schizochytrium limacinum to efficiently accumulate squalene.

The invention relates to the technical field of gene engineering, and discloses a high-strength promoter, a schizochytrium limacinum engineering bacterium based on the promoter and application of the schizochytrium limacinum engineering bacterium. Phosphoric acid pantetheinyl transferase (PPTase) is a key rate-limiting enzyme for biosynthesis of docosahexaenoic acid. Four high-strength promoters which can be strongly expressed in a schizochytrium limacinum chassis are obtained through screening and comprise a P3431 promoter, a P5659 promoter, a P6631 promoter and a P4862 promoter, and the nucleotide sequences of the promoter are shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4 respectively; furthermore, the high-strength promoter is connected with PPTase and is integrated into a schizochytrium limacinum genome, so that the yield of the docosahexaenoic acid is remarkably increased. After a P3431 promoter, a P5659 promoter, a P6631 promoter and a P4862 promoter are respectively connected with PPTase and are transferred into schizochytrium limacinum for expression, the docosahexaenoic acid yield is respectively increased by 25%, 37.5%, 43% and 36.5% compared with that of a wild type.

The present application relates to a novel strain of Schizochytrium sp. with easy intracellular oil extraction and a method for producing oil containing omega3 using same. The novel microalgae of Schizochytrium sp. according to the present invention has a high content of fat in biomass, specially a high content of unsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid, and thus it is very easy to extract biomass produced by itself or by cultivation and fermentation and fat components including unsaturated fatty acids from the biomass. Accordingly, the microalgae, and dried biomass and bio-oil prepared therefrom can be helpfully used as a composition for feed or a composition for food.

The present invention relates to a novel Schizochytrium sp. strain containing a high protein content, antioxidant pigments and omega-3 fatty acids, and a method for producing a biomass containing a high protein content, antioxidant pigments and omega-3 fatty acids by using the strain. The novel Schizochytrium sp. strain of the present invention simultaneously contains, in the cells thereof, a high content of protein ingredients including amino acids such as glutamic acid, arginine and aspartic acid, antioxidant pigments of beta-carotene and canthaxanthin, and omega-3 unsaturated fatty acid ingredients including eicosapentaenoic acid and docosahexaenoic acid, and thus the strain itself or the biomass and bio-oil produced by culturing and fermenting the strain can be effectively used as a feed composition or a food composition and the like.

The invention belongs to the technical field of microbial engineering and synthetic biology, and particularly relates to a schizochytrium limacinum mutant strain A1-11 and application thereof in synthesis of odd-carbon-chain fatty acid. According to the invention, ARTP plasma mutagenesis is carried out on wild-type Aurantiochytrium sp. Sdu080, a mutagenic strain A1-11 with genetic stability is successfully screened, and compared with an original strain, the total proportion of C15: 0 and C17: 0 of the mutagenic strain under a conventional shake-flask culture condition is increased from 7.17% to 33.13%. Furthermore, after 20 mM of L-valine is added into the fermentation liquid culture medium, the proportion of odd fatty acid of A1-11 can be further increased to 52.14%. The schizochytrium limacinum A1-11 provided by the invention not only remarkably breaks through the limitation of the odd fatty acid yield and proportion in the prior art, but also has the advantages of simple process, easiness in large-scale fermentation and the like.

The present invention relates to a novel strain of Schizochytrium sp. with easy intracellular oil extraction and a method for producing oil containing omega-3 using same. The novel microalgae of Schizochytrium sp. according to the present invention have a high fat content compared to other biomass and especially have a high content of unsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid, and thus allow easy extraction of biomass produced from the microalgae or by culturing and fermenting, and easy extraction of fat components including unsaturated fatty acid from the biomass. Therefore, the microalgae, and a dried biomass product and bio-oil produced therefrom may be utilized as a composition for feed or a composition for food.

The invention discloses a fermentation process and application of algal oil containing specific DHA distribution characteristics, and belongs to the technical field of microbial fermentation and functional algal oil preparation. According to the method, sodium citrate participating in tricarboxylic acid circulation is utilized to supplement and enhance central carbon metabolic flux, and more sufficient acetyl coenzyme A and precursor substances are provided for fatty acid synthesis, so that the fatty acid synthesis flux is up-regulated; the phytosterol is used as a cell membrane fluidity regulator, so that the activity of the Sn-2 site acyltransferase in the schizochytrium limacinum is favorably activated, and the DHA is directionally guided to be combined to the Sn-2 site of the TAG. According to the invention, the two substances are synergistically added to a schizochytrium limacinum fermentation system, so that the three targets that the total DHA content gt is 40%, the ratio of Sn-2DHA to the total DHA gt is 50% and the specific triglyceride molecule content reaches the standard can be simultaneously realized accidentally, and the problem that the DHA content and distribution are difficult to regulate and control synchronously in the prior art is effectively solved.

The invention discloses a method for increasing the yield of stearic acid in schizochytrium limacinum based on lipid droplet targeting and application, and belongs to the technical field of microbial metabolism engineering. The invention aims to solve the technical problems of non-specific positioning of single lipid droplet protein signal peptide and difficulty in efficiently producing stearic acid in schizochytrium limacinum. The invention provides a fusion protein containing a lipid droplet targeting signal peptide and palmitic acid elongase, wherein the amino acid sequence of the lipid droplet targeting signal peptide is shown as SEQ ID NO: 5, and the lipid droplet targeting signal peptide is connected to the N-terminal of the palmitic acid elongase. The fusion protein is expressed in a schizochytrium limacinum genetic engineering strain and is subjected to fermentation culture, so that the yield of stearic acid is effectively increased. The fusion protein and the method are mainly used for improving the stearic acid yield of schizochytrium limacinum, can be used for plant subcellular localization research, provide a favorable method for subcellular localization research of microalgae such as schizochytrium limacinum and have important significance.

The invention belongs to the technical field of bioengineering, and discloses recombinant schizochytrium limacinum for producing DHA (docosahexaenoic acid) lipid by using carbon dioxide as well as a construction method and application of the recombinant schizochytrium limacinum, and the recombinant schizochytrium limacinum is obtained by introducing optimized encoding genes FDH, FK, FPR and Ti related to CO2 into schizochytrium limacinum; wherein the gene sequence of the gene FDH is SEQ ID No. 17, the gene sequence of the gene FK is SEQ ID No. 18, the gene sequence of the gene FPR is SEQ ID No. 19, and the gene sequence of the gene Ti is SEQ ID No. 20. The schizochytrium limacinum engineering bacterium capable of efficiently and rapidly utilizing the CO2 is constructed through a metabolic engineering means, and the schizochytrium limacinum engineering bacterium can utilize the CO2 as a carbon source to produce the DHA lipid, so that the production cost of the DHA lipid can be reduced, and the production economy of the DHA lipid can be improved.

This invention relates to a method for preparing and applying a fermented product of Bifida Ferment Lysate and Dioscorea Oolong with enhanced moisturizing and anti-aging effects, belonging to the field of microbial fermentation technology. This invention discloses a *Bifidobacterium longum*, the nucleotide sequence of which is shown in SEQ ID NO: 1. This invention utilizes *Bifidobacterium longum* HJ-005 to ferment *Dioscorea opposita* and *Dioscorea opposita* to prepare a *Bifida Ferment Lysate* fermented product of *Dioscorea opposita*, simultaneously increasing the content of absorbable diosgenin, anthocyanins, arginine, and organic selenium, with better effects compared to other lactic acid bacteria. It can significantly reduce the water loss and shrinkage of zebrafish embryos, exhibiting a moisturizing effect, and can significantly promote the expression of zebrafish type I collagen and Elna genes, promoting collagen and elastin regeneration, thus possessing anti-aging effects and can be used as a new raw material for cosmetics.

Disclosed are a ruminant methane emission inhibitor, a preparation method thereof, and use thereof. The ruminant methane emission inhibitor of the present application is prepared from components including 1-5 parts by weight of Schizochytrium sp. powder and 0.05-0.5 parts by weight of cellulase. The ruminant methane emission inhibitor of the present application can reduce the amount of methane generated by ruminants while increasing the total fermentation activity of the rumen.

The invention relates to the technical field of microbial fermentation, and discloses a method for producing DHA algal oil through schizochytrium limacinum fermentation. The method comprises the following steps: fermenting schizochytrium limacinum in a culture medium containing glucose; the fermentation is divided into first-stage fermentation, second-stage fermentation and third-stage fermentation; in the second-stage fermentation process, glucose and acetate are supplemented into the culture medium; in the third-stage fermentation process, acetate is supplemented into the culture medium, so that the total carbon source in the culture medium is 2-5g / L; the stopping time of the first-stage fermentation is 22-26 hours, and the stopping time of the second-stage fermentation is 46-50 hours. According to the method, the yield of sn-2DHA can be effectively increased while the fermentation period is shortened.

The invention belongs to the technical field of microbial fermentation and biology, and particularly relates to a method for increasing the content of phosphatidic acid in a schizochytrium limacinum fermentation product. According to the method, genetic engineering modification is carried out on the schizochytrium limacinum, a phosphatidic acid phosphatase gene in the schizochytrium limacinum is knocked out, and the way that the schizochytrium limacinum converts phosphatidic acid to generate diglyceride is blocked, so that the purpose of increasing the content of phosphatidic acid in a schizochytrium limacinum fermentation product is achieved, and efficient production of phosphatidic acid is realized.

The present disclosure relates to a Schizochytrium sp. Strain, and a method for producing a biomass by using the strain.

The present application relates to the field of microbial technology, and particularly relates to a schizochytrium and application thereof in production of specific proportion of fatty acid. The present application screens a schizochytrium producing specific proportion of fatty acid, the content of C18:1 in the bio-oil produced by fermentation of the schizochytrium is higher, and the proportion of n-3, n-6 and n-9 is in a dominant mass ratio, which can be directly used in the production of fatty acid supplement or related products in the subsequent production process, without the need of blending other kinds of oil, the quality of raw materials is easier to control, the formula is simpler, the production efficiency and quality of the fatty acid supplement or related products can be effectively improved, and the application value is higher.

The present application relates to a novel Schizochytrium sp. strain from which intracellular oil can be easily extracted and a method for producing an oil containing omega-3 using the same, and the novel Schizochytrium sp. microalgae according to the present invention has a high fat content in the biomass, and in particular a high content of unsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid, so that it is very easy to extract the biomass itself or the biomass produced by culture and fermentation, and the fat components containing unsaturated fatty acids from the biomass. Therefore, the microalgae, the dried biomass and the bio-oil produced therefrom can be usefully used in feed compositions or food compositions.

This invention relates to the field of microbial fermentation engineering, specifically to a polyunsaturated fatty acid oil product and its preparation method. The oil product provided by this invention contains fermentation products from a strain with accession number CCTCC NO: M 2021564. The astaxanthin content in the oil product is not less than 1.0%, and the DHA:astaxanthin ratio is (10:1)–(45:1). This invention employs ion beam implantation mutagenesis combined with flow cytometry to efficiently obtain target mutant strains. High-astaxanthin mutants are screened from a large number of Schizochytrium mutant strains. The mutant strains obtained by this invention can be rapidly industrialized with low cost and high production efficiency. The Schizochytrium mutant strains provided by this invention can be used to produce oil products with high astaxanthin content as well as deeply processed products.

The application belongs to the technical field of bioengineering, and discloses a genetically engineered strain for DHA oil production of Schizochytrium sp. based on carbon flux guidance and enhanced fatty acid transport, a method and application thereof. The genetically engineered strain is obtained by introducing optimized coding genes ACLY and / or Fat1p coding genes related to carbon flux guidance and fatty acid transport into Schizochytrium sp. in vivo. The gene sequence of the gene ACLY is SEQ ID No. 9, and the gene sequence of the gene Fat1p is SEQ ID No. 10. Through metabolic engineering, the application constructs a Schizochytrium sp. engineering strain for DHA oil production based on carbon flux guidance and enhanced fatty acid transport. The Schizochytrium sp. engineering strain is based on the combination of carbon flux guidance and fatty acid transport, which can not only be used for oil synthesis by guiding carbon flux, but also avoid oil degradation by fatty acid transport.

This invention belongs to the fields of genetic engineering and fermentation engineering, and discloses a method for constructing and applying a Schizochytrium strain that produces high DHA content. The engineered Schizochytrium strain uses Schizochytrium sp. HX308 as the starting strain. Through metabolic engineering, a complete elongation-desaturation pathway is established in Schizochytrium (overexpression of C16 / 18 elongase, Δ-9 elongase gene, Δ-5 desaturase gene, Δ-17 desaturase gene, Δ-5 elongase gene, and Δ-4 desaturase gene), which works synergistically with the PKS pathway to produce DHA. The peroxisome synthesis factor PEX10 is knocked out to obtain the recombinant engineered strain SCD3. In fed-batch fermentation, the final DHA content of the engineered Schizochytrium strain SCD3 is the highest to date, reaching 70.1% of the total fatty acid content. The oil and DHA yields are also significantly increased, reaching 67.9 g / L and 47.6 g / L, respectively. The DHA yield is 1.7 times that of the wild type. This engineered strain has good application prospects.