59 results about "Schizochytrium sp." patented technology

The invention belongs to the technical field of biochemical engineering and the technical field of bioengineering, and discloses a schizochytrium limacinum engineering strain for producing EPA (eicosapentaenoic acid) by prolonging a desaturation path. Part or all of the extended desaturase genes from thraustochytrium sp. And fusarium sp. And saprolegnia are expressed. According to the invention, extended desaturase genes from various sources are integrated into a schizochytrium limacinum genome, so that a schizochytrium limacinum gene engineering strain efficiently expresses the extended desaturase genes, and a heterologous EPA synthesis path with high synthesis efficiency, short culture period, simple purification method and low cost is provided; a basis is provided for producing grease rich in EPA from schizochytrium limacinum, and the problems of non-sustainability, high cost and the like caused by obtaining EPA from fishes are solved.

The invention belongs to the technical field of bioengineering and the technical field of food engineering, and discloses a regulation and control method for improving the DHA content and the sn-2 DHA proportion in schizochytrium limacinum grease fermentation liquor, schizochytrium limacinum algae powder and application of the schizochytrium limacinum algae powder. In the fermentation culture process, the pH of fermentation liquor in the fermentation process is controlled by supplementing acid or alkali in stages. According to the method disclosed by the invention, the pH value of the fermentation liquor is regulated and controlled in stages in the fermentation culture process of the schizochytrium limacinum, and the schizochytrium limacinum can obtain a higher splitting propagation speed, a longer grease accumulation period and a shorter grease consumption end period by regulating and controlling the pH value in the fermentation process. According to the obtained schizochytrium limacinum grease, the oil yield is obviously improved compared with that of a traditional fermentation strategy, and the DHA content and the sn-2 DHA proportion in the oil are also obviously improved.

The invention belongs to the technical field of gene engineering, and discloses a schizochytrium limacinum gene engineering strain capable of efficiently producing EPA (Eicosapentaenoic Acid). The gene engineering strain is obtained by replacing key catalytic structural domains orfA, orfB-KSB / CLF in a schizochytrium limacinum PKS pathway with corresponding functional domains in a heterologous EPA type PKS pathway. The gene engineering strain can improve the proportion of polyunsaturated fatty acid in the gene engineering strain, especially eicosapentaenoic acid. EPA generated after fermentation of the obtained schizochytrium limacinum genetic engineering strain orfA:: PfaA and KSB / CLF:: PfaC reaches 4.24% and 10.19% of the total grease content respectively, the EPA content is increased by 2.52 and 7.47 times respectively, and compared with an existing schizochytrium limacinum wild type strain, the EPA yield is greatly increased, which shows that the method provided by the invention has extremely high application potential.

The invention discloses a schizochytrium limacinum genetic engineering strain capable of remodeling an FAS (Fibroblast Activated Sequence) pathway as well as a construction method and application thereof. The schizochytrium limacinum gene engineering strain is obtained by heterologous expression of a C16 elongase gene E16, a C18 elongase gene E18, a delta 9 desaturase gene D9, a delta 5 desaturase gene D5, a delta 6 desaturase gene D6 and a delta 15 desaturase gene D15 in a schizochytrium limacinum wild type. The recombinant schizochytrium limacinum described herein is capable of producing EPA comprising 2.08% by total fatty acids while also producing DHA comprising at least more than 41% by total fatty acids. In addition, the invention discloses a method for producing unsaturated fatty acid through a double-path channel of PKS and FAS, which is beneficial to promotion and application of a technology for producing EPA and DHA by schizochytrium limacinum.

The invention belongs to the technical field of biology, and discloses a method and application for regulating and controlling the fermentation process of schizochytrium limacinum through plant hormones to improve the oil titer, and the method is characterized in that in the fermentation process of schizochytrium limacinum, one or more plant hormones are added into a culture medium to promote accumulation of oil. Plant hormones are added into a fermentation culture medium of schizochytrium limacinum, the plant hormones comprise gibberellin (GB), 6-benayl aminopurine (6-BAP), forchlorfenuron (CPPU) and brassinolide (BR), and the plant hormones promote grease accumulation by adjusting activity of enzymes related to fatty acid synthesis, so that the plant hormones can adjust metabolic pathways of the schizochytrium limacinum and promote grease accumulation. The synthesis efficiency and the yield of the grease are obviously improved.

The invention discloses a method for producing docosahexaenoic acid by strengthening schizochytrium limacinum, which comprises the following steps: carrying out fermentation culture on a schizochytrium limacinum strain until the growth period is ended (a nitrogen source is used up); when the growth period of the schizochytrium limacinum is ended, exogenously adding hydrogen peroxide, culturing, exogenously adding para aminobenzoic acid, and culturing; and repeating for several times, and ending the fermentation to obtain the DHA-containing fermentation liquor. Compared with execution of a blank group without addition of exogenous additives, an independent hydrogen peroxide intermittent feeding control strategy and an independent aminobenzoic acid intermittent feeding control strategy, the DHA yield is increased by 25.21% or above when the hydrogen peroxide-p-aminobenzoic acid periodic control method is adopted, and the highest level of 1.49 g / L is achieved.

The invention relates to the technical field of microbial fermentation, and discloses application of a regulatory factor in promoting schizochytrium limacinum to produce DHA (docosahexaenoic acid), and the regulatory factor contains beta-carotene and reduced glutathione. In addition, the invention also provides a method for improving DHA production of schizochytrium limacinum and a preparation method of microbial oil. The method comprises the following steps: fermenting schizochytrium limacinum in a culture medium, and adding beta-carotene and reduced glutathione into the culture medium in the fermentation process; in the culture medium, the content of the beta-carotene is 1.5 to 2g / L. The beta-carotene and the reduced glutathione can effectively improve the content of grease and DHA prepared by fermentation of schizochytrium limacinum, the content of DHA can reach 63.9%, and the beta-carotene and the reduced glutathione are non-toxic and high in safety.

The invention belongs to the technical field of biology, and discloses a yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application of the yarrowia lipolytica engineering strain. Delta9 elongase, Delta8 desaturase, Delta5 desaturase and Delta17 desaturase genes in a fatty acid synthesis route, heterologous ATP-citrate lyase and a mitochondrial citric acid vector are overexpressed. According to the invention, ATP-citric acid lyase and a mitochondrial citric acid carrier gene which are respectively derived from Schizochylene sp. And Saccharomyces cerevisiae are jointly expressed in the yarrowia lipolytica engineering strain, so that the yields of EPA and grease in the yarrowia lipolytica engineering strain are remarkably improved, and compared with a control strain, the yields of the grease and the EPA are respectively improved by 243.35% and 161.85%.

The invention belongs to the technical field of microorganisms, and particularly relates to schizochytrium limacinum and application thereof as well as a method and a product for producing omega-3 polyunsaturated fatty acid. The schizochytrium sp. SKA-2 is preserved in the China Center for Type Culture Collection on October 23, 2024, and the preservation number of the schizochytrium sp. SKA-2 is CCTCC (China Center for Type Culture Collection) NO: M 20242314. The Schizochytrium sp. SKA-2 can grow well under the low-salt condition, and when the Schizochytrium sp. SKA-2 is used for fermentation to obtain the omega-3 polyunsaturated fatty acid, the production speed is high, the fermentation time is short, and the content of the omega-3 polyunsaturated fatty acid is high.

The present invention belongs to the technical field of microbial fermentation, and discloses a method for co-producing DHA and carotenoids by schizochytrium fermentation, wherein the method uses schizochytrium as a production strain to produce polyunsaturated fatty acids and carotenoids, adds an NADPH oxidase inhibitor to the fermentation medium, and adopts a fermentation method with staged temperature control. The present invention combines staged temperature-changing fermentation with the addition of an NADPH oxidase inhibitor during microbial fermentation, thereby achieving the co-production of DHA and carotenoids during schizochytrium fermentation, thereby obtaining microbial oils with high DHA and carotenoid content.

The invention relates to the technical field of genetic engineering, in particular to a recombinant vector, an expression plasmid, a recombinant schizochytrium limacinum mutant and a method for increasing the DHA content of microorganisms. The LIP1 gene for coding lipase in the microbial lipid droplet protein is knocked out through a homologous recombination technology, and the production of lipase in the microbial lipid droplet can be effectively inhibited, so that the hydrolysis process of triglyceride of polyunsaturated fatty acid stored in the lipid droplet to glycerol and free fatty acid in the later stage of microbial culture is blocked, and DHA is prevented from being lost from a TAG repository; experimental verification shows that in microorganisms represented by schizochytrium limacinum, the DHA content can be increased by 21.8% compared with that of a wild type, the yield and purity of DHA are increased, the stability of the microbial fermentation process is guaranteed, and application and popularization are facilitated.

A Schizochytrium algae strain rich in n-3 fatty acid and a screening and culture method therefor. Compared with a conventional strain, the content of EPA and the content of n-3 DPA in the provided strain are greatly improved, and the strain has good genetic stability, fast growth rate and good industrialization prospects. Also provided are a culture medium for mutagenesis of Schizochytrium sp., a culture medium for screening Schizochytrium sp., and a suitable mutagenesis method and screening method.

The invention belongs to the technical field of genetic engineering, and discloses a schizochytrium limacinum genetic engineering strain capable of reducing the proportion of DPA in grease and correspondingly increasing the content of DHA, a method and application of the schizochytrium limacinum genetic engineering strain. The strain is characterized in that an omega-3 desaturase gene derived from Platyneria dumeriii and / or Lepeophtheria salmonis is subjected to heterologous expression, so that the proportion of DPA (docosahexaenoic acid) in grease is reduced, and the content of DHA (docosahexaenoic acid) is correspondingly increased. According to the invention, by heterologous expression of Pd delta 19Des and Ls delta 19Des genes in schizochytrium limacinum, desaturation activity of two desaturases to a delta 19 position is verified, and conversion from omega-6 type DPA to omega-3 type DHA is effectively promoted.

The present invention belongs to the technical field of oil and fat nutritional products, and relates to a microbial oil rich in Sn-2 DHA and its preparation method and application. The microbial oil is prepared by fermentation of a Schizochytrium strain, and the Schizochytrium strain is Schizochytrium sp. D2.0, and its deposit number is CCTCCNO: M20221481. The proportion of DHA at the Sn-2 position of triglycerides in the microbial oil prepared by the present invention is not less than 50%, and the content of triglyceride DHA is not less than 58%, and it has good stability and nutritional value.

The invention belongs to the technical field of biochemical engineering, and discloses a production method for improving the content of odd-chain fatty acid in grease by modifying schizochytrium limacinum and optimizing a substrate and application of the method. According to the method, schizochytrium limacinum HX-308 serves as a receptor strain, high-yield odd-chain fatty acid engineering bacteria are obtained through gene overexpression and inhibition, and the high-yield odd-chain fatty acid is obtained. Accumulation of a propionyl CoA precursor pool for synthesizing odd-chain fatty acid is improved, endogenous methylmalonyl-CoA mutase coding gene MUT is inhibited, so that a competitive pathway of the odd-chain fatty acid is inhibited, and then through substrate optimization, the content of fermented grease and the content of the odd-chain fatty acid are obviously improved. The expression of related enzymes in schizochytrium limacinum is adjusted through a genetic engineering strategy to improve the odd-chain fatty acid synthesis, the synthesis and utilization of the key intermediate propionyl coenzyme A in the odd-chain fatty acid synthesis process are increased, the metabolic pathway of the propionyl coenzyme A is inhibited, and substrate optimization is assisted to improve the odd-chain fatty acid yield.

The invention belongs to the technical field of bioengineering, and discloses a genetic engineering strain for enhancing schizochytrium limacinum DHA oil production based on carbon flux guidance and fatty acid transport, a method and application thereof. The gene engineering strain is obtained by introducing an optimized coding gene ACLY and / or Fat1p coding gene related to guiding carbon flux and fatty acid transport into a schizochytrium limacinum body; wherein the gene sequence of the gene ACLY is SEQ ID No. 9, and the gene sequence of the gene Fat1p is SEQ ID No. 10. According to the invention, a schizochytrium limacinum engineering bacterium for enhancing DHA oil production based on carbon flux guidance and fatty acid transfer is constructed by means of metabolic engineering. The schizochytrium limacinum engineering bacterium is combined with fatty acid transfer based on carbon flux guiding, can be used for grease synthesis by guiding carbon flux, and can prevent grease from being degraded through fatty acid transfer.

The invention provides a schizochytrium limacinum mutant strain and application thereof. The schizochytrium limacinum mutant strain is a schizochytrium limacinum mutant strain CABIO-A-2-VII, the preservation number of the schizochytrium limacinum mutant strain is CCTCC M 20242741, and the schizochytrium limacinum mutant strain is preserved in the China Center for Type Culture Collection. The EPA content in polyunsaturated fatty acid grease obtained by fermentation of the schizochytrium limacinum mutant strain CABIO-A-2-VII provided by the invention is greatly increased, the C14: 0 content is higher, the DHA content in grease obtained by using the strain under a conventional fermentation process is greater than or equal to 40%, the EPA content is greater than 2%, and the C14: 0 is greater than or equal to 12% (the C14: 0 can reach 19% under an optimal fermentation process).

The invention provides a method for producing polyunsaturated fatty acid, and belongs to the technical field of biological fermentation. The method for producing polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid from schizochytrium limacinum comprises the following steps: activating schizochytrium limacinum in a seed culture medium to obtain a schizochytrium limacinum seed culture solution; inoculating the schizochytrium limacinum seed culture solution into a fermentation culture medium for culturing, wherein the fermentation culture medium comprises rapeseed protein powder; and extracting the culture solution in the cultured fermentation culture medium to obtain the polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid. According to the present invention, the yield of polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid can be significantly improved.

The invention relates to the technical field of grease processing, and discloses a method for increasing the sn-2 DHA content in schizochytrium limacinum triglyceride. The method comprises the following steps: adding a metabolic regulator into a fermentation culture solution containing schizochytrium limacinum, carrying out fermentation culture until the total fermentation time is more than 120 hours, and collecting a grease product, the metabolic regulator is selected from at least one of choline chloride, inositol and lysophosphatidic acid. According to the method provided by the invention, the lipid metabolic flux of schizochytrium limacinum can be effectively regulated and controlled, and directional enrichment of DHA to the sn-2 site of triglyceride is remarkably promoted, so that the yield and content of sn-2 DHA are improved.

The invention belongs to the technical field of biochemical engineering, and discloses a method for promoting schizochytrium limacinum to quickly accumulate Omega-3 fatty acid by using a natural antioxidant and application of the schizochytrium limacinum, the method comprises the following steps: performing fermentation culture on the schizochytrium limacinum, adding a mixed exogenous antioxidant melatonin and a rosemary extract in the fermentation culture process, and performing fermentation culture on the mixed exogenous antioxidant melatonin and the rosemary extract to obtain the Omega-3 fatty acid-containing schizochytrium limacinum. The melatonin and rosemary extract mixed antioxidant can interact to regulate fermentation in schizochytrium limacinum to increase fatty acid synthesis and oxidation stability. According to the present invention, in the experiment of schizochytrium limacinum fermentation oil production, the addition of the specific amount of the mixed antioxidant composition during the fermentation results show that the oil yield can be increased, the PUFA ratio in the specific concentration range can be significantly increased by 32%, the PUFA use amount can be reduced by 40% through the compounding use, and the method can save manpower, material resources and cost, and can provide the good application prospect. The grease production efficiency is greatly improved, and the method is suitable for industrial production.

This invention discloses a fermentation method for increasing the yield of odd-chain fatty acids from *Schizochytrium*. *Schizochytrium* is inoculated into a fermentation medium containing assimilated carbon and nitrogen sources for fermentation. The carbon sources include carbon source a (a common carbon source) and carbon source b (a precursor that can be converted into propionyl-CoA intracellularly). The fermentation is carried out in a shake flask or fermenter. When fermentation is carried out in a fermenter, carbon source b is continuously added to the fermentation system at a constant flow rate. This invention, by establishing a mixed carbon source culture system and continuously supplementing propionyl-CoA precursors during fermentation in a fermenter, reduces the transient toxicity of propionic acid, maintains cellular energy metabolism and redox homeostasis, and improves the effective supply of intracellular propionyl-CoA, thereby significantly increasing the yield of odd-chain fatty acids.

Provided herein are microorganism of a Thraustochytrid and a method for preparing bio-oil using the same, and more particularly, Schizochytrium sp. PB31 (PTA-123692) having bio-oil producibility, and a method of preparing bio-oil, particularly bio-oil having a content of omega-3 and / or 6 long-chain polyunsaturated fatty acids of 30% or more by weight or more based on total fatty acids, characterized by culturing the microorganism.

The application belongs to the technical field of biology, and discloses a Yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application, wherein a chassis strain of the engineering strain is Yarrowia lipolytica Po1f, and genes of Δ9 elongase, Δ8 desaturase, Δ5 desaturase and Δ17 desaturase in a fatty acid synthesis pathway, a heterologous ATP-citrate lyase and a mitochondrial citrate carrier are overexpressed. ATP-citrate lyases and mitochondrial citrate carrier genes respectively derived from Schizochytrium sp. and Saccharomyces cerevisiae are combined to be expressed in the Yarrowia lipolytica engineering strain, so that the production of EPA and oil in the Yarrowia lipolytica engineering strain is significantly improved, and compared with a control strain, the production of oil and EPA is respectively increased by 243.35% and 161.85%.

The invention belongs to the technical field of biochemical engineering, and discloses a method for producing squalene by inducing schizochytrium limacinum through a salt stress strategy and application, the method comprises the following steps: fermenting a schizochytrium limacinum strain for fermentation for 0-72 hours, adding 3g / L of NaCl into a fermentation medium, after 72 hours, adding NaCl into the fermentation liquid to 5g / L, continuing to ferment for 120 hours, measuring the sugar consumption every 12 hours in the fermentation process, and supplementing glucose to 80g / L. According to the method, the squalene yield of schizochytrium limacinum is remarkably increased through a salt stress strategy, an expensive inducer is prevented from being used, and the production cost is effectively reduced. 3 g / L of low-concentration salt is added in 0-72 h of the early stage of fermentation to serve as an adaptation period, so that schizochytrium limacinum grows rapidly and is induced to start an osmotic regulation mechanism, the concentration of NaCl is increased to 5 g / L in 72 h, at the moment, the schizochytrium limacinum enters a lipid accumulation period, and high-concentration NaCl is utilized to stress the schizochytrium limacinum to efficiently accumulate squalene.

The invention relates to the technical field of gene engineering, and discloses a high-strength promoter, a schizochytrium limacinum engineering bacterium based on the promoter and application of the schizochytrium limacinum engineering bacterium. Phosphoric acid pantetheinyl transferase (PPTase) is a key rate-limiting enzyme for biosynthesis of docosahexaenoic acid. Four high-strength promoters which can be strongly expressed in a schizochytrium limacinum chassis are obtained through screening and comprise a P3431 promoter, a P5659 promoter, a P6631 promoter and a P4862 promoter, and the nucleotide sequences of the promoter are shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4 respectively; furthermore, the high-strength promoter is connected with PPTase and is integrated into a schizochytrium limacinum genome, so that the yield of the docosahexaenoic acid is remarkably increased. After a P3431 promoter, a P5659 promoter, a P6631 promoter and a P4862 promoter are respectively connected with PPTase and are transferred into schizochytrium limacinum for expression, the docosahexaenoic acid yield is respectively increased by 25%, 37.5%, 43% and 36.5% compared with that of a wild type.

The present application relates to a novel strain of Schizochytrium sp. with easy intracellular oil extraction and a method for producing oil containing omega3 using same. The novel microalgae of Schizochytrium sp. according to the present invention has a high content of fat in biomass, specially a high content of unsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid, and thus it is very easy to extract biomass produced by itself or by cultivation and fermentation and fat components including unsaturated fatty acids from the biomass. Accordingly, the microalgae, and dried biomass and bio-oil prepared therefrom can be helpfully used as a composition for feed or a composition for food.

The present invention relates to a novel Schizochytrium sp. strain containing a high protein content, antioxidant pigments and omega-3 fatty acids, and a method for producing a biomass containing a high protein content, antioxidant pigments and omega-3 fatty acids by using the strain. The novel Schizochytrium sp. strain of the present invention simultaneously contains, in the cells thereof, a high content of protein ingredients including amino acids such as glutamic acid, arginine and aspartic acid, antioxidant pigments of beta-carotene and canthaxanthin, and omega-3 unsaturated fatty acid ingredients including eicosapentaenoic acid and docosahexaenoic acid, and thus the strain itself or the biomass and bio-oil produced by culturing and fermenting the strain can be effectively used as a feed composition or a food composition and the like.

The present disclosure describes an isolated Schizochytrium sp. strain and uses thereof. Also disclosed are fermentation methods, methods of producing compositions of lipids comprising docosahexaenoic acid (DHA), as well as methods of producing compositions of lipids comprising odd chain fatty acids thereof using the isolated Schizochytrium sp. strain.

The invention belongs to the technical field of microbial engineering and synthetic biology, and particularly relates to a schizochytrium limacinum mutant strain A1-11 and application thereof in synthesis of odd-carbon-chain fatty acid. According to the invention, ARTP plasma mutagenesis is carried out on wild-type Aurantiochytrium sp. Sdu080, a mutagenic strain A1-11 with genetic stability is successfully screened, and compared with an original strain, the total proportion of C15: 0 and C17: 0 of the mutagenic strain under a conventional shake-flask culture condition is increased from 7.17% to 33.13%. Furthermore, after 20 mM of L-valine is added into the fermentation liquid culture medium, the proportion of odd fatty acid of A1-11 can be further increased to 52.14%. The schizochytrium limacinum A1-11 provided by the invention not only remarkably breaks through the limitation of the odd fatty acid yield and proportion in the prior art, but also has the advantages of simple process, easiness in large-scale fermentation and the like.

The present invention relates to the technical field of microbial preparation, specifically to Schizochytrium sp. and its applications, as well as methods and products for producing EPA, DHA, and heptadecanoic acid. Schizochytrium sp. SK-3 was deposited at the China Center for Type Culture Collection on October 23, 2024, with the deposit number CCTCC NO: M 20242315. The application of Schizochytrium sp. SK-3 in the production of EPA, DHA, and high-yield heptadecanoic acid. This strain is cultured in a fermentation medium containing a carbon source, a nitrogen source, and inorganic salts at 20-30 °C for 72-96 hours to obtain oils containing long-chain fatty acids such as docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and heptadecanoic acid, which can be used to prepare food products, cosmetics, or pharmaceutical compositions for animals or humans.