16 results about "Schizochytrium sp." patented technology

The invention belongs to the technical field of bioengineering, and discloses a genetic engineering strain for enhancing schizochytrium limacinum DHA oil production based on carbon flux guidance and fatty acid transport, a method and application thereof. The gene engineering strain is obtained by introducing an optimized coding gene ACLY and / or Fat1p coding gene related to guiding carbon flux and fatty acid transport into a schizochytrium limacinum body; wherein the gene sequence of the gene ACLY is SEQ ID No. 9, and the gene sequence of the gene Fat1p is SEQ ID No. 10. According to the invention, a schizochytrium limacinum engineering bacterium for enhancing DHA oil production based on carbon flux guidance and fatty acid transfer is constructed by means of metabolic engineering. The schizochytrium limacinum engineering bacterium is combined with fatty acid transfer based on carbon flux guiding, can be used for grease synthesis by guiding carbon flux, and can prevent grease from being degraded through fatty acid transfer.

The invention relates to the technical field of grease processing, and discloses a method for increasing the sn-2 DHA content in schizochytrium limacinum triglyceride. The method comprises the following steps: adding a metabolic regulator into a fermentation culture solution containing schizochytrium limacinum, carrying out fermentation culture until the total fermentation time is more than 120 hours, and collecting a grease product, the metabolic regulator is selected from at least one of choline chloride, inositol and lysophosphatidic acid. According to the method provided by the invention, the lipid metabolic flux of schizochytrium limacinum can be effectively regulated and controlled, and directional enrichment of DHA to the sn-2 site of triglyceride is remarkably promoted, so that the yield and content of sn-2 DHA are improved.

This invention discloses a fermentation method for increasing the yield of odd-chain fatty acids from *Schizochytrium*. *Schizochytrium* is inoculated into a fermentation medium containing assimilated carbon and nitrogen sources for fermentation. The carbon sources include carbon source a (a common carbon source) and carbon source b (a precursor that can be converted into propionyl-CoA intracellularly). The fermentation is carried out in a shake flask or fermenter. When fermentation is carried out in a fermenter, carbon source b is continuously added to the fermentation system at a constant flow rate. This invention, by establishing a mixed carbon source culture system and continuously supplementing propionyl-CoA precursors during fermentation in a fermenter, reduces the transient toxicity of propionic acid, maintains cellular energy metabolism and redox homeostasis, and improves the effective supply of intracellular propionyl-CoA, thereby significantly increasing the yield of odd-chain fatty acids.

Provided herein are microorganism of a Thraustochytrid and a method for preparing bio-oil using the same, and more particularly, Schizochytrium sp. PB31 (PTA-123692) having bio-oil producibility, and a method of preparing bio-oil, particularly bio-oil having a content of omega-3 and / or 6 long-chain polyunsaturated fatty acids of 30% or more by weight or more based on total fatty acids, characterized by culturing the microorganism.

The application belongs to the technical field of biology, and discloses a Yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application, wherein a chassis strain of the engineering strain is Yarrowia lipolytica Po1f, and genes of Δ9 elongase, Δ8 desaturase, Δ5 desaturase and Δ17 desaturase in a fatty acid synthesis pathway, a heterologous ATP-citrate lyase and a mitochondrial citrate carrier are overexpressed. ATP-citrate lyases and mitochondrial citrate carrier genes respectively derived from Schizochytrium sp. and Saccharomyces cerevisiae are combined to be expressed in the Yarrowia lipolytica engineering strain, so that the production of EPA and oil in the Yarrowia lipolytica engineering strain is significantly improved, and compared with a control strain, the production of oil and EPA is respectively increased by 243.35% and 161.85%.

The invention relates to the technical field of gene engineering, and discloses a high-strength promoter, a schizochytrium limacinum engineering bacterium based on the promoter and application of the schizochytrium limacinum engineering bacterium. Phosphoric acid pantetheinyl transferase (PPTase) is a key rate-limiting enzyme for biosynthesis of docosahexaenoic acid. Four high-strength promoters which can be strongly expressed in a schizochytrium limacinum chassis are obtained through screening and comprise a P3431 promoter, a P5659 promoter, a P6631 promoter and a P4862 promoter, and the nucleotide sequences of the promoter are shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4 respectively; furthermore, the high-strength promoter is connected with PPTase and is integrated into a schizochytrium limacinum genome, so that the yield of the docosahexaenoic acid is remarkably increased. After a P3431 promoter, a P5659 promoter, a P6631 promoter and a P4862 promoter are respectively connected with PPTase and are transferred into schizochytrium limacinum for expression, the docosahexaenoic acid yield is respectively increased by 25%, 37.5%, 43% and 36.5% compared with that of a wild type.

The present application relates to a novel strain of Schizochytrium sp. with easy intracellular oil extraction and a method for producing oil containing omega3 using same. The novel microalgae of Schizochytrium sp. according to the present invention has a high content of fat in biomass, specially a high content of unsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid, and thus it is very easy to extract biomass produced by itself or by cultivation and fermentation and fat components including unsaturated fatty acids from the biomass. Accordingly, the microalgae, and dried biomass and bio-oil prepared therefrom can be helpfully used as a composition for feed or a composition for food.

The present invention relates to a novel Schizochytrium sp. strain containing a high protein content, antioxidant pigments and omega-3 fatty acids, and a method for producing a biomass containing a high protein content, antioxidant pigments and omega-3 fatty acids by using the strain. The novel Schizochytrium sp. strain of the present invention simultaneously contains, in the cells thereof, a high content of protein ingredients including amino acids such as glutamic acid, arginine and aspartic acid, antioxidant pigments of beta-carotene and canthaxanthin, and omega-3 unsaturated fatty acid ingredients including eicosapentaenoic acid and docosahexaenoic acid, and thus the strain itself or the biomass and bio-oil produced by culturing and fermenting the strain can be effectively used as a feed composition or a food composition and the like.

The present invention relates to a novel strain of Schizochytrium sp. with easy intracellular oil extraction and a method for producing oil containing omega-3 using same. The novel microalgae of Schizochytrium sp. according to the present invention have a high fat content compared to other biomass and especially have a high content of unsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid, and thus allow easy extraction of biomass produced from the microalgae or by culturing and fermenting, and easy extraction of fat components including unsaturated fatty acid from the biomass. Therefore, the microalgae, and a dried biomass product and bio-oil produced therefrom may be utilized as a composition for feed or a composition for food.

The invention discloses a method for increasing the yield of stearic acid in schizochytrium limacinum based on lipid droplet targeting and application, and belongs to the technical field of microbial metabolism engineering. The invention aims to solve the technical problems of non-specific positioning of single lipid droplet protein signal peptide and difficulty in efficiently producing stearic acid in schizochytrium limacinum. The invention provides a fusion protein containing a lipid droplet targeting signal peptide and palmitic acid elongase, wherein the amino acid sequence of the lipid droplet targeting signal peptide is shown as SEQ ID NO: 5, and the lipid droplet targeting signal peptide is connected to the N-terminal of the palmitic acid elongase. The fusion protein is expressed in a schizochytrium limacinum genetic engineering strain and is subjected to fermentation culture, so that the yield of stearic acid is effectively increased. The fusion protein and the method are mainly used for improving the stearic acid yield of schizochytrium limacinum, can be used for plant subcellular localization research, provide a favorable method for subcellular localization research of microalgae such as schizochytrium limacinum and have important significance.

The invention belongs to the technical field of bioengineering, and discloses recombinant schizochytrium limacinum for producing DHA (docosahexaenoic acid) lipid by using carbon dioxide as well as a construction method and application of the recombinant schizochytrium limacinum, and the recombinant schizochytrium limacinum is obtained by introducing optimized encoding genes FDH, FK, FPR and Ti related to CO2 into schizochytrium limacinum; wherein the gene sequence of the gene FDH is SEQ ID No. 17, the gene sequence of the gene FK is SEQ ID No. 18, the gene sequence of the gene FPR is SEQ ID No. 19, and the gene sequence of the gene Ti is SEQ ID No. 20. The schizochytrium limacinum engineering bacterium capable of efficiently and rapidly utilizing the CO2 is constructed through a metabolic engineering means, and the schizochytrium limacinum engineering bacterium can utilize the CO2 as a carbon source to produce the DHA lipid, so that the production cost of the DHA lipid can be reduced, and the production economy of the DHA lipid can be improved.

This invention relates to a method for preparing and applying a fermented product of Bifida Ferment Lysate and Dioscorea Oolong with enhanced moisturizing and anti-aging effects, belonging to the field of microbial fermentation technology. This invention discloses a *Bifidobacterium longum*, the nucleotide sequence of which is shown in SEQ ID NO: 1. This invention utilizes *Bifidobacterium longum* HJ-005 to ferment *Dioscorea opposita* and *Dioscorea opposita* to prepare a *Bifida Ferment Lysate* fermented product of *Dioscorea opposita*, simultaneously increasing the content of absorbable diosgenin, anthocyanins, arginine, and organic selenium, with better effects compared to other lactic acid bacteria. It can significantly reduce the water loss and shrinkage of zebrafish embryos, exhibiting a moisturizing effect, and can significantly promote the expression of zebrafish type I collagen and Elna genes, promoting collagen and elastin regeneration, thus possessing anti-aging effects and can be used as a new raw material for cosmetics.

Disclosed are a ruminant methane emission inhibitor, a preparation method thereof, and use thereof. The ruminant methane emission inhibitor of the present application is prepared from components including 1-5 parts by weight of Schizochytrium sp. powder and 0.05-0.5 parts by weight of cellulase. The ruminant methane emission inhibitor of the present application can reduce the amount of methane generated by ruminants while increasing the total fermentation activity of the rumen.

This invention relates to the field of microbial fermentation engineering, specifically to a polyunsaturated fatty acid oil product and its preparation method. The oil product provided by this invention contains fermentation products from a strain with accession number CCTCC NO: M 2021564. The astaxanthin content in the oil product is not less than 1.0%, and the DHA:astaxanthin ratio is (10:1)–(45:1). This invention employs ion beam implantation mutagenesis combined with flow cytometry to efficiently obtain target mutant strains. High-astaxanthin mutants are screened from a large number of Schizochytrium mutant strains. The mutant strains obtained by this invention can be rapidly industrialized with low cost and high production efficiency. The Schizochytrium mutant strains provided by this invention can be used to produce oil products with high astaxanthin content as well as deeply processed products.

The application belongs to the technical field of bioengineering, and discloses a genetically engineered strain for DHA oil production of Schizochytrium sp. based on carbon flux guidance and enhanced fatty acid transport, a method and application thereof. The genetically engineered strain is obtained by introducing optimized coding genes ACLY and / or Fat1p coding genes related to carbon flux guidance and fatty acid transport into Schizochytrium sp. in vivo. The gene sequence of the gene ACLY is SEQ ID No. 9, and the gene sequence of the gene Fat1p is SEQ ID No. 10. Through metabolic engineering, the application constructs a Schizochytrium sp. engineering strain for DHA oil production based on carbon flux guidance and enhanced fatty acid transport. The Schizochytrium sp. engineering strain is based on the combination of carbon flux guidance and fatty acid transport, which can not only be used for oil synthesis by guiding carbon flux, but also avoid oil degradation by fatty acid transport.

This invention belongs to the fields of genetic engineering and fermentation engineering, and discloses a method for constructing and applying a Schizochytrium strain that produces high DHA content. The engineered Schizochytrium strain uses Schizochytrium sp. HX308 as the starting strain. Through metabolic engineering, a complete elongation-desaturation pathway is established in Schizochytrium (overexpression of C16 / 18 elongase, Δ-9 elongase gene, Δ-5 desaturase gene, Δ-17 desaturase gene, Δ-5 elongase gene, and Δ-4 desaturase gene), which works synergistically with the PKS pathway to produce DHA. The peroxisome synthesis factor PEX10 is knocked out to obtain the recombinant engineered strain SCD3. In fed-batch fermentation, the final DHA content of the engineered Schizochytrium strain SCD3 is the highest to date, reaching 70.1% of the total fatty acid content. The oil and DHA yields are also significantly increased, reaching 67.9 g / L and 47.6 g / L, respectively. The DHA yield is 1.7 times that of the wild type. This engineered strain has good application prospects.