High-yield DHA (Docosahexaenoic Acid) strain and application thereof

A high-yield, strain technology, applied in fungi, microorganisms, fermentation and other directions, can solve the problems that DHA cannot meet the needs of society, pollute the environment, limited resources, etc., and achieve the effect of broad industrial application prospects.

Active Publication Date: 2014-08-13
SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional source of DHA is deep-sea fish oil, but this source has relatively prominent defects: limited resources, complicated extraction process, polluting the environment, and containing a strong fishy smell
Therefore, DHA derived from traditional deep-sea fish is far from being able to meet the needs of society.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 , HCCB30108 screening

[0018] After centrifuging the water samples collected in the coastal waters of Zhoushan, Ningbo, the sediment was spread on a solid medium containing penicillin (0.5g / L) and streptomycin (0.5g / L) (glucose 30g / L, yeast extract 5g / L , peptone 10g / L, agar 20g / L, seawater, pH 7.0), cultured at 28°C for 4-5 days. The obtained culture was transferred to a new solid plate, and the plate was streaked until a single colony appeared. After re-screening, a pure culture was finally obtained, numbered HCCB30108.

Embodiment 2

[0019] Example 2 , Identification of HCCB30108

[0020] The HCCB30108 obtained in Example 1 was sent to the Institute of Microbiology, Chinese Academy of Sciences for strain identification. According to the data of HCCB30108 culture and cell micromorphological characteristics, physiological and biochemical characteristics and rRNA gene sequence, it was determined that HCCB30108 was Schizochytrium sp.

[0021] Schizochytrium HCCB30108 was deposited on October 28, 2011 in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No.5415.

Embodiment 3

[0022] Example 3 , Fermenter culture of Schizochytrium CGMCC No.5415

[0023] The Schizochytrium CGMCC No.5415 obtained in Example 2 was fermented. The slant medium is: glucose 30g / L, yeast powder 5g / L, peptone 10g / L, sea salt 15g / L, agar powder 20g / L, pH7.0. The 2-day-old Schizochytrium was inoculated into the seed medium. The seed medium is: glucose 30g / L, yeast extract 5g / L, peptone 10g / L, sea salt 15g / L, pH 7.0. Cultivate at 25°C until the logarithmic growth phase, and inoculate 5L fermented culture medium in the fermenter. The fermentation medium is: glucose 60g / L, corn steep liquor 12g / L, sea salt 15g / L, pH 6.0. The inoculation amount is 10%, the stirring speed is controlled at 250-300rpm, the temperature is 25°C, the speed is adjusted to control the dissolved oxygen to 40%, the air flow rate is 3L / min, and the pH is maintained at 6.0 by using ammonia water. For 0-18 hours of fermentation, feed 600g / L glucose solution at a rate of 2.5mL / h; for 18-48 hours, feed 600...

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PUM

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Abstract

The invention discloses a Schizochytrium sp. CGMCC No.5415 of a high yield of DHA (Docosahexaenoic Acid) as well as a fermentation method and an application of Schizochytrium sp. CGMCC No.5415. The biomass and the DHA can be rapidly accumulated by utilizing the Schizochytrium sp. CGMCC No.5415. Thus, the Schizochytrium sp. CGMCC No. 5415 has a wide industrial application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-yielding bacterial strain of docosahexaenoic acid and an application thereof. Background technique [0002] DHA (docosahexaenoic acid) is an important long-chain polyunsaturated fatty acid (PUFA), belonging to the ω3 series. DHA has many important physiological functions such as promoting the growth and development of brain cells, lowering blood fat, anti-inflammation, improving autoimmunity, anti-cancer, promoting nerve development and improving vision. It is known as a new generation of functional health care factors and is greatly appreciated by the world. focus on. [0003] The traditional source of DHA is deep-sea fish oil, but this source has relatively prominent defects: limited resources, complicated extraction process, polluting the environment, and containing a strong fishy smell. Therefore, DHA derived from traditional deep-sea fish is far from being a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/64C12R1/645
Inventor 金文翔阮丽军杨志钧夏兴戈梅孙新强金一平张定丰王刚
Owner SHANGHAI LAIYI BIOMEDICAL RES & DEV CENT
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