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Method for producing lutein from microalgae

A technology of lutein and microalgae, applied in the direction of fermentation, can solve the problems of restricting the industrialization process of lutein, high cost of large-scale cultivation of microalgae, low lutein content and lutein yield, etc. The effect of high quality, rapid accumulation and lower production cost

Active Publication Date: 2011-06-15
EAST CHINA UNIV OF SCI & TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It can be seen from the above that whether the photoautotrophic culture mode or the heterotrophic culture mode is adopted, the low intracellular lutein content and lutein yield, coupled with the high cost of large-scale culture of microalgae, restrict the application. Industrialization process of microalgae culture to produce lutein

Method used

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  • Method for producing lutein from microalgae
  • Method for producing lutein from microalgae
  • Method for producing lutein from microalgae

Examples

Experimental program
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Effect test

Embodiment 1

[0075] Add the following heterotrophic medium and water to 2.8L in a 5L bioreactor, then steam sterilize it, then insert Chlorella pyrenoidosa when the temperature drops to 30°C, and start heterotrophic culture.

[0076] Heterotrophic culture conditions: the temperature is 30±1°C, the air flow rate is 1vvm, the pH is less than 8.0, and the dissolved oxygen is controlled above 15%.

[0077] Feed was fed for the first time 53.9 hours after inoculation, and then fed every 5 to 8 hours for a total of 4 times, until the dry weight of cells reached 132.2g / L at 88.40 hours (see figure 1 ), at this time the heterotrophic culture was completed and transferred to light-induced culture.

[0078] Dilute the high-density algae liquid after the heterotrophic culture to 2.55g / L, add the following light-induced medium, and transfer it to a 3L flat-plate photobioreactor for light-induced culture. Light-induced culture conditions: the temperature is maintained at 28-33°C, the air flow rate is ...

Embodiment 2

[0090] Add the following heterotrophic medium and tap water to a 50L bioreactor to 25L, then sterilize at 121°C for 20min, then insert Chlorella pyrenoidosa according to 10% of the working volume when the temperature drops to about 30°C, and start heterotrophic Raise and cultivate.

[0091] Heterotrophic culture conditions: the temperature is 30°C, the air flow rate is 1vvm, the pH is 6.0-8.0, and the dissolved oxygen is controlled above 15%. During the cultivation process, when the glucose was consumed, the carbon source was supplemented, and when the urea was consumed, the nitrogen source was supplemented. After adding carbon source 6 times, the algae cell density reached 130.5g / L at 98.89h after adding nitrogen source 3 times (see image 3 ).

[0092] Dilute the high-density algae after heterotrophic culture to 2.01g / L, add light-induced medium, and transfer to a 10L cylindrical photobioreactor for light-induced induction.

[0093] Light-induced culture conditions: natur...

Embodiment 3

[0095] Add the following heterotrophic medium and tap water to a 50L bioreactor to 25L, then sterilize at 121°C for 20 minutes, then insert Chlorella vulgaris into 13% of the working volume when the temperature drops to about 30°C, and start heterotrophy to cultivate.

[0096] Heterotrophic culture conditions: temperature is 30°C, air flow is 1vvm, pH is less than 9.0. During the cultivation process, when the carbon source was exhausted, glucose was supplemented, and when the nitrogen source was exhausted, potassium nitrate was supplemented. By adding carbon source and nitrogen source 5 times, the algae cell density reached 54.5g / L at 58.20h (see Figure 5 ).

[0097] Dilute the heterotrophically cultured high-density algae to about 3.2g / L, add light-induced medium, and transfer to a 30L flat-plate photobioreactor for light-induced culture outdoors. Light-induced culture conditions: natural temperature, natural light, and air flow of 1.0vvm. After 28 hours of light inducti...

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Abstract

The invention relates to a method for quickly accumulating intracellular lutein of microalgae, comprising the following steps of: culturing by heterotrophism, diluting, culturing by photoinduction, collecting microalgae, extracting lutein and the like. With the method, the advantages of the quick accumulation of lutein of the microalga cell obtained by culturing by heterotrophism in the photoinduction stage can be fully exerted, and an important technical means is provided for the industrialization of the lutein derived from the microalgae.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and relates to a method for producing lutein by culturing microalgae. Background technique [0002] Lutein, also known as "plant lutein", is a natural active substance widely present in vegetables, flowers, fruits and other plants and algae. Lutein is currently widely used as a food and feed additive because it has many important physiological effects on the human body, such as protecting eyesight, delaying arteriosclerosis, anti-cancer, anti-oxidation, and protecting the skin. Color enhancer for poultry, aquaculture animals and animal tissue. [0003] The wide application prospect of lutein in medicine and health care products, food and feed, cosmetics and aquaculture industry has made many domestic and foreign research institutions and companies conduct research on the biosynthesis, separation and extraction, physiological and biochemical functions of lutein, etc. . At present, lutein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00
Inventor 李元广黄建科王伟良范建华李淑兰魏鸿刚沈国敏李际军
Owner EAST CHINA UNIV OF SCI & TECH
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