490 results about "Penicillin" patented technology

The invention discloses an improved YOLOv8-based penicillin bottle body defect detection method, and belongs to the technical field of computer vision, and the method comprises the following steps: obtaining and preprocessing penicillin bottle body defect data to obtain a training set and a test set; establishing a defect target detection model of the improved YOLOv8; training a defect target detection model by adopting the training set, optimizing a loss function, and updating a model weight parameter until the loss function is converged; and testing the defect target detection model by adopting the test set. According to the invention, a space separable pooling attention module SSPA is introduced, so that the perception capability of the network on subtle features such as bottle body scratches can be enhanced; a feature enhancement module MMFF is introduced, convolution kernels of different sizes are adopted to extract defect features of different sizes, such as smudginess, scratches and loading quantity anomaly, under a complex background, and the feature fusion effect is improved by module stacking.

The invention discloses a culture medium and application thereof, and a method for in-vitro separation culture and adipogenic differentiation of precursor adipocytes in goat muscle, and relates to the technical field of cell culture, the culture medium comprises the following components: 8-12 [mu] g / mL of insulin, 8-12% by volume of fetal calf serum, 95-105 U / mL of penicillin, 95-105 [mu] g / mL of streptomycin and the like. On the basis of the prior art, a proper amount of liquiritin and pilocarpine are mainly added into a culture medium, and synergistic cooperation of the liquiritin and pilocarpine is found, so that the adipogenic differentiation capacity of cells such as mesenchymal stem cells and precursor adipocytes is remarkably improved, and the application range is wide. On the basis, the invention also provides a complete set of perfect in-vitro isolated culture and adipogenic differentiation method of the goat intramuscular precursor adipocytes, and a method and a way are provided for a series of researches developed through the goat primary intramuscular precursor adipocytes in the future.

The invention provides a preparation method of Al18F tracer integrated freeze-dried powder, a kit and application thereof, and the preparation method comprises the following steps: dissolving 1-10 mg of a precursor by using deionized water, adding a pH buffer solution with the concentration of 0.2-0.5 M and an AlCl3 solution with the concentration of 10 mM, with the ratio of the amount of substance of AlCl3 to the amount of substance of the precursor being 0.8-1.0, then continuously adding a stabilizer solution with the concentration of 0.5 M and 1-4 mg of an excipient, and uniformly stirring to obtain the Al18F tracer integrated freeze-dried powder. And finally, adding deionized water to enable the concentration of the precursor to be 0.5-1.0 mM, uniformly mixing the solution, sub-packaging the solution into 1.5 mL cryopreservation tubes or 2 mL penicillin bottles according to a certain volume, and then putting the cryopreservation tubes or the penicillin bottles into a vacuum freeze dryer to remove water at a low temperature to obtain powder or freeze-dried cakes, and the container is a freeze-dried powder / cake storage container and is used for storing the freeze-dried powder / cakes. Meanwhile, the reaction container is also used during radioactive labeling.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The application belongs to the technical field of medicine production equipment, and relates to a full-automatic double-conveying high-speed sterile medicine powder filling machine with adjustable penicillin bottle specifications, which comprises a control system and a rack, the rack is provided with a first bottle feeding system, a powder hitting system, a weighing system, a sealing system and a first conveying line, the first conveying line is provided with a first left drive, a first right drive and a bottle clamping module, the rack is further provided with a second bottle feeding system and a second conveying line, the second conveying line is provided with a second right drive and a second left drive, the first bottle feeding system, the second bottle feeding system, the first conveying line and the second conveying line are synchronously operated, the machine further comprises a rejection system and a bottle discharging and opening system, and the powder hitting system, the weighing system, the sealing system, the rejection system and the bottle discharging and opening system on the first conveying line and the second conveying line are synchronously operated. The application solves the problem of limited machine speed caused by single-line production, and realizes full-automatic switching of penicillin bottle production of different specifications.

The invention discloses a liver progenitor organoid culture medium and a culture method, and belongs to the technical field of organoid culture. The culture medium comprises a basic culture medium, Jagged-1, Dexamehysone, TGF (Transforming Growth Factor)-alpha, beta-mercaptoethanol, BMP4 (Bone Morphogenetic Protein 4), FGF2 (Fibroblast Growth Factor 2), specific addition factors Glutamax, HEPES (Hydroxyethyl PES), Nicotinamide, Penicillin and the like. The liver progenitor organoid is constructed by using the liver tissue for the first time, continuous growth and efficient proliferation of human liver progenitor cells in vitro can be promoted under the condition that gene editing is not carried out, meanwhile, the pluripotency of the liver progenitor organoid is maintained, and the consistency of specific marker expression is maintained; and a novel batch model is provided for the evaluation of hepatotoxicity at the present stage. From the long-term view, a foundation is laid for constructing a liver organ model with more advanced various differentiated cells.

The utility model belongs to the technical field of penicillin bottle packaging, and discloses an automatic penicillin bottle packaging device which comprises a penicillin bottle feeding system, a material arranging system, a paper box feeding system, a plastic support feeding system and a boxing system, and the penicillin bottle feeding system is used for conveying and feeding penicillin bottles; the sorting system is used for receiving the penicillin bottles and grouping the plurality of penicillin bottles into a plurality of rows and a plurality of columns; the paper box feeding system is used for opening the folded paper boxes and conveying the folded paper boxes to the boxing area. The plastic support feeding system is used for placing a plastic support in a paper box; and the boxing system is used for grabbing multiple rows and columns of penicillin bottles and putting the penicillin bottles on the plastic support in the paper box to complete packaging. According to the penicillin bottle feeding device, automatic conveying and feeding of penicillin bottles are achieved, a plurality of penicillin bottles can be grouped into multiple rows and multiple columns, synchronous operation of paper box feeding and plastic support feeding can be achieved, then multiple rows and multiple columns of penicillin bottles can be placed at a time for packaging, labor is reduced, and packaging efficiency is greatly improved.

The invention discloses a human small cell lung cancer brain metastasis organoid culture medium and a culture method. According to the culture medium, an advanced DMEM / F12 culture medium is used as a basic culture medium, and the following components are added: 2 to 5 [mu] M of GlutaMax, 8 to 10 [mu] M of HEPES, 1 * to 2 * of B27, 500 [mu] M of SB202190, 5 to 10 [mu] M of Y-27632, 0.5 [mu] M of A83-01, 0.5 to 2 [mu] M of N-Acetyl-L-cysteine, 5 mM of Nicotinamide, 25 to 100 ng / ml of recombinant human FGF7, 100 to 500 ng / ml of recombinant human FGF10, 100 to 200 ng / ml of Human Noggin, 50 to 500 ng / ml of Human R-Spondin-1, 50 to 100 ng / ml of According to the culture method, TryPLE Express is used for dissociation, the time is short, the activity of dissociated single cells is high, and the success rate of organoid culture can be increased to the maximum extent. Meanwhile, by combining with a culture medium with an optimized formula proportion, formation and growth of brain metastatic organs of the human small cell lung cancer can be promoted, and the construction success rate is increased.

The present invention belongs to the field of biotechnology, and specifically relates to the use of the CD630_27900 gene in reducing the tolerance of Clostridium difficile. Knocking out the CD630_27900 gene can reduce the tolerance of Clostridium difficile to acidic environments and antibiotics and reduce its cytotoxicity; the sequence of the CD630_27900 gene is shown in SEQ ID NO.1; the antibiotics include ampicillin, metronidazole, amoxicillin, vancomycin, norfloxacin, cefoxitin, clindamycin and kanamycin. The research results of the present invention suggest that the CD630_27900 gene can be used as a target for inhibiting the drug resistance and reducing the toxicity of Clostridium difficile, providing a new idea for the treatment of Clostridium difficile infection (CDI).

The present invention discloses a buffer for tissue separation and / or preservation and its application, and a method for culturing tumor organoids, and relates to the field of organoid culture. The buffer is used to preserve tumor tissue samples, which are of liquid origin; the buffer includes: a basal culture medium and specific additive factors, and the specific additive factors include: superoxide dismutase, catalase, penicillin, and streptomycin; based on the volume of the basal culture medium, the concentration of superoxide dismutase is 1 to 100 μg / mL, the concentration of catalase is 50 to 500 μg / mL, the concentration of penicillin is 50 to 200 U / mL, and the concentration of streptomycin is 50 to 200 μg / mL. The tissue preservation solution of the present invention is used for the transportation and protection of liquid-derived tumor tissue samples, and has a high tumor cell activity retention rate. It is used to construct tumor organoids with a high success rate and a large number, which can meet the needs of clinical detection and disease research.

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

The invention provides an iron-doped copper nanocluster nano-enzyme, a preparation method thereof and application of the iron-doped copper nanocluster nano-enzyme to detection of penicillin sodium by a colorimetric method. According to the preparation method, PVP-K30 and metal chlorate are taken as initial raw materials, deionized water is taken as a solvent environment, and the iron-doped copper nanocluster nanoenzyme is prepared through oil bath heating synthesis and dialysis purification. When 3, 3 ', 5, 5'-tetramethyl benzidine-hydrogen peroxide reaction is catalyzed by the nano-enzyme, the nano-enzyme is found to have an excellent peroxidase simulation effect, and rapid colorimetric detection of penicillin sodium is realized. The nano-enzyme provides an important thought for the development of novel nano-enzymes, and has a potential application value for the detection of antibiotics in a complex matrix.

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

The invention discloses a prebiotic composition capable of proliferating intestinal beneficial flora and a preparation method thereof, and the prebiotic composition comprises the following raw materials in parts by weight: 35-45 parts of partially hydrolyzed complex gum, 30-40 parts of green banana resistant starch, 15-25 parts of enzymolysis okra polysaccharide and 3-5 parts of tea polyphenol. According to the composition, the partially hydrolyzed complex gum with specific molecular weight, the green banana resistant starch, the enzymolysis okra polysaccharide and the tea polyphenol are scientifically compounded, so that efficient proliferation of intestinal effective microbial communities and comprehensive and positive regulation and control of intestinal microenvironment are realized, and the composition is prepared by utilizing four components with complementary functions and different effects. The bacillus amyloliquefaciens can effectively proliferate traditional beneficial bacteria such as bifidobacteria and lactobacillus, can effectively nourish butyric acid producing bacteria such as clostridium praeparatum and inhibit growth of harmful bacteria, so that the bacillus amyloliquefaciens has the function of remarkably improving intestinal flora disorder caused by ampicillin, and has the functions of promoting generation of short-chain fatty acid and maintaining intestinal barriers.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention provides a method for deeply degrading penicillin V through combined circulating cascade of paracoccus and aspergillus niger and application of the method, and belongs to the technical field of antibiotic pollutant degradation, paracoccus is paracoccus sp KDSPL-02, aspergillus niger is Aspergillus niger, and the method is used for deeply mineralizing and degrading penicillin V to dissolve possible pollution caused by the paracoccus sp KDSPL-02 and the aspergillus niger is Aspergillus niger. Waste hyphae generated in the production process are subjected to harmless treatment.

The invention relates to a phenylacetylglutamine / phenylacetylglycine induced vascular smooth muscle cell senescence model and an establishment method and application thereof, and belongs to the technical field of cell models.The method comprises the following steps that vascular smooth muscle cell suspension is placed in a porous plate, 10% of FBS and 1% of mycillin are added into a culture medium, 24 h after cells adhere to the wall, the vascular smooth muscle cell senescence model is obtained, and the vascular smooth muscle cell senescence model is obtained. The method comprises the following steps of: adding a proper amount of phenylacetyl glutamine or phenylacetyl glycine dimethyl sulfoxide solution into a porous plate, changing a serum-free culture medium, continuously culturing for 24 hours, adding a proper amount of phenylacetyl glutamine or phenylacetyl glycine dimethyl sulfoxide solution into the porous plate, changing the culture medium added with phenylacetyl glutamine or phenylacetyl glycine once every 24 hours, and collecting cells after 72 hours. According to the invention, phenylacetylglutamine / phenylacetylglycine is used for inducing and establishing the vascular smooth muscle cell senescence model for the first time, the method is easy to operate and low in cost, and a foundation is laid for researching the relationship between cardiovascular diseases and senescence.

The invention discloses a high-specific-activity glucose oxidase mutant as well as a construction method and antibacterial application thereof, and belongs to the technical field of bioengineering. Glucose oxidase GODY is used as a female parent, Ala143 is mutated into Leu143, then a mutant A143L is obtained, and the specific activity of the mutant A143L is improved by 3.1 times compared with that of a wild type; in the aspect of thermal stability, the T50 value and the half-life period at 75 DEG C of the mutant A143L are respectively increased by 6 DEG C and 9 minutes compared with those of the wild mutant A143L; in the aspect of antibacterial performance, the IC50 value of the mutant A143L to staphylococcus aureus is 19.2 mg / L, and the antibacterial performance of the mutant A143L is obviously superior to that of a wild type and is equivalent to that of ampicillin. The acquisition of the mutant with high catalytic activity provides theoretical reference for the improvement of the catalytic performance of enzyme, and the mutant A143L with strong antibacterial performance shows huge application prospects in antibiotic substitution.

The present invention belongs to the technical field of semen cryopreservation, and more specifically, relates to sheep semen freezing solution and semen preservation method. The sheep semen freezing solution components of the present invention include Tris, citric acid, fructose, lactose, trehalose, penicillin, streptomycin, soy lecithin, nobiletin, glycerol and water. By using a specific content of nobiletin as an effective antioxidant component of a sheep sperm cryopreservative, it plays an antioxidant role when the sperm is frozen, making the sperm more stable during the cryopreservation process. After adding nobiletin, the vitality and motility of sheep sperm after freezing and thawing can be significantly improved. It effectively reduces the oxidative stress damage of sperm during the freezing process and improves the antioxidant capacity of sperm.

The invention relates to the field of medical liquid preparation, in particular to a medicine bottle shaking-up device for full-automatic high-speed liquid preparation equipment and the liquid preparation equipment. The medicine bottle shaking-up device comprises a medicine bottle shaking-up device body and the liquid preparation equipment, and the medicine bottle shaking-up device body is provided with a translation guide rail and a clamping installation table connected to the translation guide rail in a sliding mode; the clamping mounting table is driven by a crank rocker mechanism to do translational reciprocating motion, a penicillin bottle clamping device and an ampoule bottle clamping device are arranged on the table, and stop stations for feeding a medicine bottle and injecting liquid medicine are arranged in the motion stroke; the liquid dispensing equipment comprises a rack, a medicine bottle library, a liquid bag library, a needle cylinder library and the like, and the medicine dispensing process is completed through manipulators and equipment. According to the device, liquid medicine in an ampoule bottle is automatically sucked and injected into medicine powder in a penicillin bottle, then the crank and rocker mechanism does linear reciprocating motion to achieve mixing and uniform shaking of the medicine powder and the liquid medicine in the penicillin bottle, manual uniform shaking is replaced, and technical support is provided for batch, efficient and automatic medicine dispensing.

The utility model discloses a storage device for prolonging the shelf life of penicillin enzyme preparations, which comprises a storage box, the upper surface of the storage box is rotatably connected with a box cover through a hinge, the side inner wall of the storage box is fixedly connected with a partition plate, the side inner wall of the storage box is provided with a storage rack, and the upper surface of the storage rack is provided with a placing opening. The upper portion of the storage box is rotationally connected with a cover plate through a hinge, the lower bottom wall of the storage box is fixedly connected with a refrigerator, the output end of the refrigerator is fixedly connected with a conveying pipe, the lower surface of the storage box is fixedly connected with a motor, the output end of the motor is fixedly connected with a rotating shaft, and the end, away from the motor, of the rotating shaft is fixedly connected with fan blades. The reagent storage rack is simple in structure and convenient to operate, interference to reagents on other storage racks is reduced, the risk of reagent cross contamination caused by misoperation is reduced, reagent storage safety can be improved, and meanwhile reagent management orderliness is enhanced.

The distributing mechanism for penicillin bottle feeding comprises a workbench, the distributing mechanism is arranged on one side of the upper portion of the workbench, the distributing mechanism comprises a material distributing mechanism and a material stirring mechanism, the material distributing mechanism comprises a material vibrating module and a material arranging module, and the material vibrating module comprises a material distributing platform; a hopper, a material disc and a feeding table are sequentially arranged above the material distributing platform from front to back. The upper portion of the feeding table is connected with an inverted-L-shaped material blocking plate in a sliding mode, and the front end of the feeding table is blocked by the material blocking plate to form a feeding track. According to the penicillin bottle feeding device, the plurality of material distributing plates arranged above the material disc slide in a front-back staggered manner and are matched with the material disc in a vibration state, so that the disordered penicillin bottles are longitudinally arranged on the feeding track, and then the penicillin bottles on the track are pushed into the material overturning channel through the push plate; when the penicillin bottles are stacked, the turning channel is opened outwards, the directions of the penicillin bottles are turned through the gravity of the penicillin bottles, the penicillin bottles are tidily stacked on the conveying belt with the heads facing upwards, the automation degree is high, the penicillin bottles are stacked more tidily, and the stacking efficiency is high.

The utility model discloses a feeding assembly, and relates to the field of penicillin bottle conveying and feeding. The feeding assembly comprises a feeding table and a folding support, a feeding groove is formed in the upper end of the feeding table, the feeding groove is of a necking structure in the feeding direction of the feeding groove, and the feeding table comprises a bottom wall and a side baffle which are fixedly connected; the folding support is provided with a rotating side and a fixed side and comprises a limiting piece. A user pushes the penicillin bottles to enable the penicillin bottles to move in the feeding direction, the movement of the penicillin bottles is guided through the necking structure, the penicillin bottles are limited through the side baffles, the penicillin bottles can be gradually fed to the conveying table, the risks of bottle falling and bottle breaking are avoided, the conveying requirement is met, and the stability of the feeding stage is improved advantageously; when the feeding table is not used, the rotary connection of the rotary side and the fixed side is unlocked through the limiting piece, so that the feeding table is folded and stored, and the occupied space is reduced; the folding chair is flexible and convenient to use, can be unfolded or folded according to requirements, and is favorable for improving the working efficiency.

The invention discloses a culture medium for preparing a human-derived Merkel cell model and a construction method, and relates to the technical field of biologication.The key points of the technical scheme are that the culture medium comprises a culture medium E, and the culture medium E is formed by adding PRCi into an OMM culture medium; the OMM is prepared by adding GlutaMax (GlutaMax), B-27 (B-27) Minus, N2 (N2) suppliers, Penicilin-Streptomycin and 2-Mercaptoethanol into an Advanced DMEM (Dulbecco's Modified Eagle Medium) / F12 (Fullcap 12) culture medium and a Neurobasic culture medium, and the OMM is prepared by adding GlutaMax, B-27 Minus, N2 (N2) suppliers, Penicilin-Streptomycin and 2-Mercaptoethanol into a medium; and the PRCi comprises EED226, PRT4165 and GSK126 (glutamine synthase 126). According to the invention, a small molecule compound combination PRCi (EED226 + PRT4165 + GSK126) is added into a culture medium, so that the amount of MC in the organoid can be remarkably increased, and a human-derived system is established for researching the MC.

The present invention discloses an IS26-based resistance gene control plasmid, engineering bacteria, bacterial agent and application, belonging to the technical field of genetic engineering. The nucleotide sequence of the IS26-based resistance gene control plasmid is shown in SEQ ID NO: 1 or SEQ ID NO: 2. The engineering bacteria and bacterial agent containing the resistance gene control plasmid can be used for the control of resistance genes. By specifically destroying the insertion sequence IS26, the number of resistance genes can be fundamentally reduced and the spread of resistance genes can be blocked, providing a precise solution for the treatment of drug resistance. It has broad-spectrum resistance bacteria control performance and can effectively control gentamicin, tetracycline, chloramphenicol and ampicillin-resistant bacteria in wastewater, with the highest control efficiency reaching 83.76%. The plasmid, engineering bacteria and bacterial agent have low preparation costs, can be used for in-situ treatment of wastewater, have low treatment costs and good effects, and have broad application prospects.

The invention belongs to the technical field of product separation in enzymatic synthesis of antibiotics, and particularly relates to a method for separating and purifying amoxicillin and phenylacetic acid in the production process of amoxicillin. An immobilized penicillin acylase mutant is used for catalyzing penicillin potassium to synthesize amoxicillin in one step, a set of method for separating amoxicillin and phenylacetic acid is developed for an obtained reaction mixture, and the technical scheme mainly comprises the following steps: firstly, separating the immobilized penicillin acylase mutant from a reaction solution through filtration; then, amoxicillin is separated out through crystallization; and then crystallizing, separating and recovering phenylacetic acid through toluene extraction, back extraction and the like. According to the separation method, the amoxicillin can be rapidly and efficiently separated, the yield of the amoxicillin is relatively high, and the average crystallization rate reaches 93.22%; meanwhile, the separation and recovery effects of phenylacetic acid are good; and the extraction agent toluene can be recycled.

The present application relates to the field of biotechnology, in particular to a carbapenem-resistant escherichia coli phage and application thereof.A new escherichia coli phage vB_EOP_Se009 is isolated and purified, and the phage has good sterilization effect on carbapenem-resistant escherichia coli.The phage has strong tolerance to temperature and pH, is high in specificity and stability, can effectively solve the problem that carbapenem-resistant escherichia coli is resistant to multiple antibiotics, and provides an experimental basis for developing a preparation for preventing or treating carbapenem-resistant escherichia coli infection in clinic, and has great clinical application potential.

This invention provides the medical use of tyrosol in the preparation of formulations that reduce the toxicity of penicillin-thiazolic acid (PTA). It discloses a novel medical use of tyrosol in preparing formulations that reduce PTA toxicity. By using tyrosol to prevent and treat the residual toxicity of PTA, unnecessary antibiotic replacement regimens can be avoided, significantly reducing the economic burden. A method for preventing and treating PTA residual toxicity is provided, which can significantly reduce the toxicity of PTA and has good preventive and therapeutic effects. The prevention and treatment of PTA residual toxicity has significant socio-economic implications for controlling the hygiene and quality of animal-derived foods, protecting consumer health, and ensuring industrial production.

The utility model relates to the related technical field of penicillin bottles, in particular to a penicillin bottle sterilizing device which comprises a sterilizing box, a placing box is connected to the inner wall of the sterilizing box in a sliding mode, a fixing assembly is arranged on the inner wall of the placing box, and a water collecting assembly is arranged at the bottom of the placing box. Through the arrangement of the fixing assembly, the size of the space formed by the mounting plate and the opening of the movable plate can be adjusted by pulling the movable handle at the top of the movable plate, the opening in one side of the mounting plate is semicircular, and the movable plate is matched with the mounting plate; the first rubber block and the second rubber block at the opening are used for clamping the circular outer wall of the penicillin bottle through the relative movement of the first rubber block and the second rubber block at the opening, so that the penicillin bottle is fixed, the penicillin bottle is prevented from being collided and damaged in the sterilization process, the device can adapt to penicillin bottles with different outer diameter specifications, and in the penicillin bottle sterilization process, high-temperature steam can generate condensed water when encountering cold, so that the safety of the penicillin bottle is improved. Through the arrangement of the water collecting assembly, condensed water can be conveniently cleaned after sterilization is finished, and the condensed water is prevented from being accumulated in the device.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.