37 results about "Penicillin" patented technology

This invention provides a nitrogen filling needle structure for vials, comprising a filling needle body, a first through hole penetrating its upper and lower end faces, and a filling needle body gas chamber inside the filling needle body; a top connector communicating with the first through hole at the upper end of the filling needle body; a second through hole penetrating its bottom end face at the bottom of the filling needle body, communicating with the filling needle body gas chamber; a first vent hole on the filling needle body, through which high-pressure gas passes sequentially via the top connector, the first through hole, the second through hole, and the first vent hole; a cap is fixedly installed on the bottom end face of the filling needle body at the top, with an opening at the bottom of the cap, and an annular rubber ring is provided on the inner wall of the cap, the annular rubber ring communicating with the interior of the filling needle body. This invention provides a nitrogen filling needle structure for vials, which can effectively prevent contamination of the liquid inside the vial during nitrogen filling, thereby improving the yield of finished products.

This invention discloses a method for isolating and extracting *Havnia vesicularis* using penicillin and vancomycin. The method mainly includes the following steps: inclusion and screening of patients with bipolar disorder; collection and processing of feces from these patients; isolation and treatment of *Havnia vesicularis* by adding penicillin and vancomycin to the feces; and identification of *Havnia vesicularis*. This invention improves the isolation efficiency and success rate of *Havnia vesicularis*, solving the problems of low isolation efficiency and poor identification accuracy in existing technologies.

The present invention belongs to the technical field of sensorineural hearing. Provided is the use of SPTBN1 as a target in the treatment of GJB2-related sensorineural hearing loss. The use comprises: the construction of a stably transfected cell line, wherein: HEK293T cells are cultured using a DMEM culture medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin in a humidified incubator at 37ºC with 95% air and 5% CO2, and when the cells reach 80%-90% confluence, cell passage is performed; IP-MS analysis, wherein: protein complexes are purified using Protein A / G immunoprecipitation magnetic beads, a portion of the extracted proteins is used as input, and then 2 μg of anti-Cx26 antibody is added to the remaining protein extract, same are gently pipetted and mixed, and incubated on a rotating shaker at 4℃ overnight; immunofluorescence observation of the co-localization of Cx26 and SPTBN1, wherein: a stably transfected cell line expressing WT-Cx26 and Mut-Cx26 is constructed; and co-immunoprecipitation (Co-IP) validation, wherein: a stably transfected cell line expressing WT-Cx26 is constructed. The present invention overcomes the limitations of therapeutic methods, such as the relatively low targeting specificity and short therapeutic time windows associated with full-length protein supplementation via gene therapy, thereby providing new insight into GJB2-related hearing loss, and a new target and a new treatment for same.

1. The name of the design product: medicine cabinet. 2. The use of the design product: for storing medicine loaded in penicillin bottles or ampoule bottles. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

This invention relates to the field of gastric cancer organoid microarray technology, and more particularly to a gastric cancer organoid microarray culture medium and its applications. The invention provides a novel culture medium adapted for gastric cancer organoid microarray culture. The medium components include basal medium, penicillin / streptomycin dual antibiotics, antibacterial agents, hydroxyethylpiperazine ethanesulfonic acid, B-27 additive, Glutamax culture medium additive, R-vertebral protein 1, scutellarin B, and tetramethylpyrazine, among other components. This invention solves key problems in existing technologies such as slow growth, morphological instability, poor heterogeneity adaptation, and low long-term culture activity of gastric cancer organoids, providing an ideal experimental model for high-throughput screening and precision medicine applications on microarrays.

The present application relates to a kind of penicillin bottle production line, including lamp inspection component, into the component and packaging component;The lamp inspection component is set in one end of the into the component, for the appearance detection of penicillin bottle;The into the component is used to place multiple penicillin bottles into box;The packaging component is set in the other end of the into the component, for the packaging of penicillin bottle after into box;The end of the lamp inspection component away from the into the component is docked penicillin bottle production device, and penicillin bottle extraction mechanical hand is set beside the penicillin bottle production device.The purpose of the present application is to overcome the existing defects and provide a kind of penicillin bottle production line, and multiple processes are connected to continuous production, which avoids manual operation.

The application discloses a fermentation method for producing thiamine pyrophosphokinase and belongs to the technical field of bioengineering. The BL21 recombinant engineering bacteria containing pET28a-TPK, pET28a-ProS2-TPK or pET28a-SUMO-TPK are constructed, the seed liquid is prepared, the isopropyl-beta-D-thiogalactoside is used for inducing expression, the bacteria are collected, the cells are broken and the supernatant is collected to obtain the TPK crude enzyme liquid. In the fermentation, the LB / TB culture medium containing 50 μg / mL kanamycin or 100 μg / mL ampicillin is used. The SUMO label is introduced to guide the correct folding of the TPK, so that the soluble expression ratio of the TPK is more than 85%, the ratio is improved by 2 times compared with the common system, the inclusion body ratio is greatly reduced, the process is stable and has good repeatability, and an effective scheme is provided for the industrialized large-scale preparation of the TPK.

The application discloses a glucose oxidase mutant with simultaneously improved thermal stability and acid resistance and antibacterial application thereof, and belongs to the field of genetic engineering and protein engineering. The application takes glucose oxidase AiGODL as a mother to obtain a mutant AiGODL_E148K / F283Y by respectively mutating Glu148 and Phe283 into Lys148 and Tyr283. It is found through experiments that, compared with wild-type glucose oxidase AiGODL, the mutant AiGODL_E148K / F283Y has improved heat resistance and acid resistance, and has the same level of specific activity and catalytic efficiency as AiGODL and the same antibacterial performance as ampicillin. The mutant AiGODL_E148K / F283Y provided by the application has great application prospect in antibacterial and antibiotic substitution.

This invention belongs to the field of appearance inspection technology, and relates to a method and system for inspecting the appearance of vials based on multi-view reflection suppression. The method includes: acquiring multi-view videos of vials passing through the inspection area and establishing sub-video groups; selecting representative images; locating the effective area of ​​the vial body and establishing a unified unfolded domain to obtain an unfolded image of the vial wall; decomposing the unfolded image of the vial wall into reflection and defect characterizations, and performing gating correction based on reflection risk to obtain single-view candidate defect characterizations; performing propagation matching based on defect prototype maps to obtain single-view category responses; performing cross-view consistency verification and outputting the inspection results. The technical solution of this application, through multi-view video organization analysis and differentiation and correction of reflection interference and defect responses, achieves stable identification and consistent judgment of vial defects, improving defect discrimination stability, detection result consistency and reliability, and meeting the application needs of automated vial inspection in pharmaceutical packaging production lines.

1. Name of the product in this design: Intelligent Drug Dispensing Machine. 2. Purpose of this design: To automate the mixing of solvent and penicillin. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 1.

The application discloses a dog-derived Enterococcus faecalis ZJUIDS-D016 with anti-aging effect and application, the dog-derived Enterococcus faecalis ZJUIDS-D016 is classified and named as (Enterococcus faecalis) Enterococcus faecalis ), and the preservation number is CGMCC 27038.The strain of the application is isolated from the intestinal tract of a healthy dog, has acid resistance, bile salt resistance and broad-spectrum antibacterial activity, and is sensitive to chloramphenicol, erythromycin and penicillin.The strain of the application has strong host adaptability, can colonize in the intestinal tract of a dog and secrete organic acids and bacteriocins, significantly inhibits the proliferation of common pathogenic bacteria such as dog pathogenic escherichia coli, effectively improves the intestinal health of pets and delays aging, and is suitable for preparing pet probiotic products.

This invention provides a method for preparing penicillin bacterial residue-based dual-activated nitrogen-oxygen co-doped biochar. The method involves: freezing penicillin bacterial residue, followed by vacuum freeze-drying to obtain dried penicillin bacterial residue; pulverizing and sieving the dried residue to obtain penicillin bacterial residue powder; mixing anhydrous ferric chloride, potassium oxalate, and the penicillin bacterial residue powder to obtain a mixture; heating the mixture to 400-800℃ under a nitrogen atmosphere and isothermal pyrolysis; naturally cooling to room temperature; and then grinding and sieving to obtain penicillin bacterial residue-based dual-activated nitrogen-oxygen co-doped biochar. An application is also provided: this penicillin bacterial residue-based dual-activated nitrogen-oxygen co-doped biochar is used for the treatment of antibiotic wastewater. This invention uses penicillin bacterial residue as a precursor and employs anhydrous ferric chloride and potassium oxalate dual activation to prepare nitrogen-oxygen co-doped biochar, and applies it to the treatment of antibiotic wastewater, realizing the resource utilization of bacterial residue.

The utility model relates to a ampoule penicillin integrated bottle protection device relates to the field of food, pharmaceutical packaging machinery, it includes table board, is equipped with the conveying mechanism for conveying ampoule bottle or penicillin bottle on the table board, and the conveying mechanism includes the bottle protection system for preventing ampoule bottle or penicillin bottle to pour, the bottom of table board is equipped with the drive system for driving conveying mechanism, and the bottle protection system is equipped with the bottle rotating system for ampoule bottle wire drawing sealing process, the inlet end of conveying mechanism is equipped with the flexible material inlet plum-pudding baffle piece. The utility model has the remarkable cost advantage, high efficiency and easy use, and has important practical significance to improve the production efficiency, product quality and economic benefit of liquid medicine production enterprise.

The utility model provides a kind of tearable double-body cover for penicillin bottle and penicillin bottle, belong to medical equipment field, it includes sealing plug and protective cover, sealing plug is used to block bottle mouth, protective cover includes protruding part, side wall and top cover, protruding part protrudes to penicillin bottle, sealing plug is integrally covered in the outer periphery of protruding part, the side of sealing plug away from protruding part is sealingly connected with the inner along of bottle mouth, the inner side of side wall is integrally connected with the edge of protective cover, and the inner along of side wall bottom is clamped in the outer periphery of bottle mouth, top cover is covered in side wall top, and the inner along of top cover is clamped with the outer periphery of side wall top, the hardness of side wall and the hardness of protective cover are all greater than the hardness of sealing plug;Side wall is equipped with two adjacent easy-to-tear recesses, and the easy-to-tear recesses penetrate the outer periphery of side wall along the axis of side wall.Compared with prior art, the utility model improves the bottle cap, solves the technical problems of the existing penicillin bottle cap operation cumbersome and hidden danger.

The utility model relates to a technical field of opening lid of a westlin bottle, and disclose an automatic uncapping device of aluminium -plastic cover for westlin bottle in the utility model, including support base, the support base top is provided with uncapping mechanism, the uncapping mechanism top is provided with fixed establishment, the uncapping mechanism includes operation panel, the operation panel fixed connection in support base top, the operation panel bottom is fixedly connected with first servo motor, first servo motor top is fixedly connected with rotating lever, the rotating lever top is fixedly connected with the placing table, the placing table top is fixedly connected with the backboard, the operation panel right side is fixedly connected with second servo motor, when using, through setting uncapping mechanism, first will multiple westlin bottles be placed respectively in the placing groove on the top of the placing table, and contact each other with the backboard, according to the size of westlin bottle simultaneously, through second servo motor drive first threaded rod periphery screw thread connection's moving seat moves, can move the left and right positions of the uncapping ware.

This invention relates to the field of microbial technology, specifically to Lactobacillus paracasei ZK-35 and its applications. The Lactobacillus paracasei ZK-35 of this invention is isolated from human oral rinsing fluid, exhibiting good compatibility with the human intestinal environment and high safety. It possesses excellent acid and bile salt resistance, and can alleviate the side effects of various antibiotics such as penicillins, cephalosporins, and quinolones. It can simultaneously regulate multiple intestinal flora, improve constipation and diarrhea, enhance intestinal barrier function, and promote nutrient absorption.

The utility model discloses a full -automatic multi -position H type flowing electrolytic cell experimental platform is with cleaning device relates to electrolytic cell experimental cleaning technical field, including experimental platform, the inside fixed mounting of experimental platform has the limit recessed block, the inside of limit recessed block is provided with transmission assembly, be provided with bottle seat on transmission assembly, the inside splicing of bottle seat has the penicillin bottle, the rear end fixed of experimental platform is provided with vertical guide rail, one side swing of vertical guide rail is provided with slide, the top of slide is provided with cleaning assembly, and the cleaning assembly is located the top of penicillin bottle. This cleaning device passes through transmission assembly, the cooperative cooperation of vertical guide rail and cleaning assembly, has realized the full -process automation operation of penicillin bottle from conveying, electrolyte extraction, water flushing to cleaning fluid extraction, and the manual intervention is greatly reduced, and the labor intensity is reduced, and the cleaning efficiency is improved significantly.

This invention discloses a method for microscopically separating and rescuing cells from heavily contaminated cell culture media, belonging to the field of biotechnology. This invention combines flow cytometry sorting technology with penicillin-streptomycin for the separation, rescue, and culture of healthy cells. The method provided by this invention can effectively rescue heavily contaminated cells, remove their contamination state, and make them usable again. This will greatly facilitate cell preservation, passage, and culture, effectively avoid the waste of cell resources, and improve the preservation rate of rare cell resources, providing strong support for the smooth progress of scientific research.

The utility model discloses a kind of filling head structures of penicillin bottle filling machine, it is related to penicillin bottle filling machine technical field, including filling head main part, the filling head main part output end is fixedly installed with filling pipe, the filling pipe output end is provided with rotating discharge component;The rotating discharge component includes fixed head, fixed pipe, waterproof bearing, fixed nut, meshing thread, rotating shell and several discharge pipes;In the technical scheme provided by the utility model, by setting rotating discharge component, specifically, when filling, filling pipe discharges, liquid is discharged by the discharge pipe of rotating shell side wall, under the reaction force of liquid discharge, rotating shell is rotated, rotating flow is generated in the process of canning, it is helpful that the component in solution is more evenly distributed in penicillin bottle, reduce the possibility of solution stratification or precipitation.

The application discloses a feeding device for a penicillin bottle of an upper-cover-opening cartoning machine, which comprises a whole arrangement mechanism and a material moving mechanism arranged at the output end of the whole arrangement mechanism; the output end of the whole arrangement mechanism is provided with a receiving plate for placing a whole arranged penicillin bottle group; the material moving mechanism is provided with a picker matched with the penicillin bottle group; the picker can move in three-dimensional directions under the driving of a driving assembly; the first direction of the three-dimensional directions is consistent with the advancing direction of a target packaging box on the upper-cover-opening cartoning machine; the second direction is consistent with the depth direction of the target packaging box; the third direction is perpendicular to the first direction and the second direction respectively; the picker can be synchronized with the target packaging box in the first direction, so as to pick up the penicillin bottle group from the receiving plate and transfer the penicillin bottle group into the target packaging box on the upper-cover-opening cartoning machine.

The application discloses a kind of Vibration Mountain beard toad hind leg / toe tissue cells and its quick acquisition method and application.The method comprises: the body surface disinfection of Vibration Mountain beard individual, cut and take hind leg or toe tissue, and put back after wound disinfection;After tissue is soaked in 75% alcohol and washed with PBS containing penicillin, streptomycin and amphotericin B, it is temporarily stored in 4 ℃ temporary storage solution;After tissue is cut, it is sequentially digested with trypsin and type I collagenase;In the optimized low permeability medium, primary culture is carried out at 25-27 ℃;When cell confluence reaches 60%-70%, it is passaged using trypsin-EDTA digestion;Freezing and recovery.The application realizes the non-lethal cell of Vibration Mountain beard for the first time Quick acquisition, cell first time migration time is only 3 days, grow into monolayer only 12-17 days, pollution rate is reduced to 5.6%, recovery survival rate reaches 76.19%, and provides efficient and reliable technical support for endangered amphibian germplasm resource preservation and biological research.

The utility model discloses a kind of penicillin bottle interval blanking assembly, it is related to blanking assembly technical field, including the support vertical pole connected to the top of the both sides of conveying belt, two the support vertical pole top is connected with crosspiece, one of the support vertical pole is connected with feed pipe, the end of the feed pipe away from support vertical pole is connected with penicillin bottle forming equipment, the end of the feed pipe towards conveying belt is set down obliquely, penicillin bottle slides from the inside of feed pipe to conveying belt, the crosspiece is connected with fixed plate, the bottom of the fixed plate is connected with motor. A kind of penicillin bottle interval blanking assembly of the utility model, with the effect of slowly reducing penicillin bottle height, and stably placing it on the top of conveying belt, avoid the situation that penicillin bottle jumps when falling on steel mesh conveying belt, simultaneously, can avoid the situation that two bottle body distance is too close due to penicillin bottle jumping and moving.

The present application belongs to the technical field of complex enzyme. The present application provides a beta-lactamase complex enzyme capable of degrading various antibiotics, and a fixing method and application thereof. The preparation method of the complex enzyme comprises the following steps: extracting gene fragments of CTX-M type broad-spectrum beta-lactamase and VIM type metal-beta-lactamase from Klebsiella pneumoniae; obtaining the target gene fragments through PCR amplification, and constructing into a basic vector to obtain a recombinant vector; transforming the recombinant vector into host cells for heterologous expression; and through protein induction, SDS-PAGE protein gel electrophoresis and protein purification, the complex enzyme is obtained. The complex enzyme successfully degrades penicillins such as ampicillin, carbapenems such as imipenem, cephalosporins such as cefotaxime and other beta-lactams. The complex enzyme gene is successfully expressed in Escherichia coli, and the optimal fixing condition is obtained. The beta-lactamase complex enzyme is fixed under the optimal fixing condition, and can be repeatedly used.

The application discloses a Treponema pallidum penicillin-related gene global mutation map construction method, comprising the following steps: S1, constructing a global representative Treponema pallidum isolate genome dataset, S2, extracting the coding sequences of four penicillin-related genes and performing pretreatment, S3, identifying and screening non-synonymous mutation sites of the penicillin-related genes, S4, jointly predicting the function influence of the non-synonymous mutations by using SIFT and PROVEAN two servers, S5, using the MLST framework combined with metadata, S6, analyzing the lineage specificity and geographical distribution characteristics of the mutations, S7, integrating and analyzing the results, S8, applying the global mutation map to beta-lactam drug resistance research, establishing a standardized technical process, each step has a clear operation standard and a verification link, effectively reducing the interference of false positive mutations, integrating the lineage specificity, geographical aggregation and function characteristics of the mutations, constructing a comprehensive global mutation map, and clearly defining high-frequency mutation sites.

This invention discloses a method and application for rapid propagation of teak clonal lines through tissue culture, belonging to the field of plant tissue culture technology. The method uses teak segments as explants and detoxifies them using solutions of alcohol, mercuric chloride, penicillin, and streptomycin. This method effectively removes bacteria and endophytes from the surface of the explants, filling a gap in current methods for detoxifying teak explants. After obtaining tissue culture seedlings, they are propagated using a bud proliferation medium, yielding a large number of plants in a short period. By adding plant growth regulators to the medium, rooting of the tissue culture seedlings is promoted. A rooting medium formula for teak tissue culture seedlings has been developed, enabling rooting within the tissue culture bottle, solving the long-standing problem of difficult rooting of teak tissue culture seedlings, and significantly improving the survival rate after transplanting.

The application discloses a Group 4 subtype medulloblastoma microtumor PTC model and a construction and evaluation method and application thereof, and specifically comprises the following steps: establishing a Group 4 subtype medulloblastoma microtumor model, using a basic culture medium containing advanced DMEM / F12 and adding specific components (glutamine, penicillin-streptomycin-amphotericin B, B27 without vitamin A, N2, 2-mercaptoethanol, non-essential amino acids, insulin, etc.) as the culture medium; processing medulloblastoma tumor tissues by a dispersion method, combining a suspension culture system and a hypoxic environment, and culturing tumor single-cell suspensions obtained through digestion in vitro to form a medulloblastoma microtumor PTC model containing various tumor tissue-derived cell types under predetermined conditions. The model is highly similar to the original tumor tissue in terms of histopathological characteristics and molecular genetic characteristics, and highly restores the microenvironment characteristics of the original tumor tissue, and can be used for drug screening, prediction of treatment response and the like.

This invention provides a novel peptide adjuvant CAL185, its composition, and its application. The peptide adjuvant CAL185 and its series derivatives have the following sequences: X0-X1-X2-X3-X4-X5-X6-X 7‑ X8-X9-X 10 -X 11 -X 12 The non-ribosomal peptide adjuvant of this invention can bind to penicillin-binding protein 2a, targeting its allosteric site, and assisting β-lactam antibiotics (such as penicillins, cephalosporins, and carbapenems) in binding to the active site of penicillin-binding protein 2a, thereby enhancing their original low affinity, blocking cell wall synthesis, reversing drug resistance, and exerting a potent bactericidal effect. The peptide adjuvant of this invention, through synergy with the aforementioned antibiotics, exhibits potent bactericidal activity against clinically common methicillin-resistant Staphylococcus aureus, and holds promise for further development into a novel anti-infective drug adjuvant.

The utility model discloses a double cofferdam sealing penicillin bottle belongs to penicillin bottle field, it includes: penicillin bottle body and connecting shell, penicillin bottle body outside fixedly connected with base, the base in -side slidingly connected with two no.

The utility model discloses a kind of penicillin bottle quick-release bottle caps, it is related to the field of medical devices, it includes bottle cap and sealing gasket, the bottle cap includes outer cover and inner cover, outer cover is used to connect penicillin bottle mouth portion, sealing gasket is set in outer cover inboard, outer cover pushes sealing gasket and carries out plugging to penicillin bottle mouth, inner cover is located in outer cover inboard, and inner cover is attached along the side edge of circumferential with outer cover, outer cover upper end portion is opened and has through opening, inner cover carries out plugging to through opening, outer cover upper end portion is fixed with pull plate, pull plate is located in through opening in natural state, outer cover side edge is opened and has two easy tear openings, two easy tear openings are located pull plate two sides respectively, and extend along pull plate length direction. The present application has the effect of improving the convenience of opening cover.