411 results about "Penicillin" patented technology

The invention discloses an improved YOLOv8-based penicillin bottle body defect detection method, and belongs to the technical field of computer vision, and the method comprises the following steps: obtaining and preprocessing penicillin bottle body defect data to obtain a training set and a test set; establishing a defect target detection model of the improved YOLOv8; training a defect target detection model by adopting the training set, optimizing a loss function, and updating a model weight parameter until the loss function is converged; and testing the defect target detection model by adopting the test set. According to the invention, a space separable pooling attention module SSPA is introduced, so that the perception capability of the network on subtle features such as bottle body scratches can be enhanced; a feature enhancement module MMFF is introduced, convolution kernels of different sizes are adopted to extract defect features of different sizes, such as smudginess, scratches and loading quantity anomaly, under a complex background, and the feature fusion effect is improved by module stacking.

The invention discloses a culture medium and application thereof, and a method for in-vitro separation culture and adipogenic differentiation of precursor adipocytes in goat muscle, and relates to the technical field of cell culture, the culture medium comprises the following components: 8-12 [mu] g / mL of insulin, 8-12% by volume of fetal calf serum, 95-105 U / mL of penicillin, 95-105 [mu] g / mL of streptomycin and the like. On the basis of the prior art, a proper amount of liquiritin and pilocarpine are mainly added into a culture medium, and synergistic cooperation of the liquiritin and pilocarpine is found, so that the adipogenic differentiation capacity of cells such as mesenchymal stem cells and precursor adipocytes is remarkably improved, and the application range is wide. On the basis, the invention also provides a complete set of perfect in-vitro isolated culture and adipogenic differentiation method of the goat intramuscular precursor adipocytes, and a method and a way are provided for a series of researches developed through the goat primary intramuscular precursor adipocytes in the future.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The application belongs to the technical field of medicine production equipment, and relates to a full-automatic double-conveying high-speed sterile medicine powder filling machine with adjustable penicillin bottle specifications, which comprises a control system and a rack, the rack is provided with a first bottle feeding system, a powder hitting system, a weighing system, a sealing system and a first conveying line, the first conveying line is provided with a first left drive, a first right drive and a bottle clamping module, the rack is further provided with a second bottle feeding system and a second conveying line, the second conveying line is provided with a second right drive and a second left drive, the first bottle feeding system, the second bottle feeding system, the first conveying line and the second conveying line are synchronously operated, the machine further comprises a rejection system and a bottle discharging and opening system, and the powder hitting system, the weighing system, the sealing system, the rejection system and the bottle discharging and opening system on the first conveying line and the second conveying line are synchronously operated. The application solves the problem of limited machine speed caused by single-line production, and realizes full-automatic switching of penicillin bottle production of different specifications.

The invention discloses a human small cell lung cancer brain metastasis organoid culture medium and a culture method. According to the culture medium, an advanced DMEM / F12 culture medium is used as a basic culture medium, and the following components are added: 2 to 5 [mu] M of GlutaMax, 8 to 10 [mu] M of HEPES, 1 * to 2 * of B27, 500 [mu] M of SB202190, 5 to 10 [mu] M of Y-27632, 0.5 [mu] M of A83-01, 0.5 to 2 [mu] M of N-Acetyl-L-cysteine, 5 mM of Nicotinamide, 25 to 100 ng / ml of recombinant human FGF7, 100 to 500 ng / ml of recombinant human FGF10, 100 to 200 ng / ml of Human Noggin, 50 to 500 ng / ml of Human R-Spondin-1, 50 to 100 ng / ml of According to the culture method, TryPLE Express is used for dissociation, the time is short, the activity of dissociated single cells is high, and the success rate of organoid culture can be increased to the maximum extent. Meanwhile, by combining with a culture medium with an optimized formula proportion, formation and growth of brain metastatic organs of the human small cell lung cancer can be promoted, and the construction success rate is increased.

The invention discloses a zebra fish spermatogonial stem cell separation and transplantation method, and belongs to the technical field of fish germline stem cell separation. The method comprises the following steps: washing complete sexual glands of zebra fish testis by adopting penicillin / streptomycin double-antibody-containing PBS (Phosphate Buffer Solution), physically shearing, digesting and dissociating, and filtering by a screen, so as to obtain zebra fish testis cell suspension; performing density gradient centrifugation to obtain zebra fish spermatogonial stem cells; dyeing, marking, centrifuging, washing, re-suspending, and carrying out micro-injection transplantation to enter the back of the abdominal cavity of the acceptor zebrafish juvenile fish. According to different physical properties of different cells, spermatogonial stem cells are separated, purified and transplanted from zebra fish testis cell suspension by adopting a density gradient centrifugation method, and migration and proliferation processes of donor germ cells can be observed in receptor fish after fluorescence labeling. And a new technical path is provided for fish germplasm resource preservation, endangered fish protection, economic cultured fish yield increase and new variety cultivation.

The invention provides an iron-doped copper nanocluster nano-enzyme, a preparation method thereof and application of the iron-doped copper nanocluster nano-enzyme to detection of penicillin sodium by a colorimetric method. According to the preparation method, PVP-K30 and metal chlorate are taken as initial raw materials, deionized water is taken as a solvent environment, and the iron-doped copper nanocluster nanoenzyme is prepared through oil bath heating synthesis and dialysis purification. When 3, 3 ', 5, 5'-tetramethyl benzidine-hydrogen peroxide reaction is catalyzed by the nano-enzyme, the nano-enzyme is found to have an excellent peroxidase simulation effect, and rapid colorimetric detection of penicillin sodium is realized. The nano-enzyme provides an important thought for the development of novel nano-enzymes, and has a potential application value for the detection of antibiotics in a complex matrix.

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

The invention discloses a prebiotic composition capable of proliferating intestinal beneficial flora and a preparation method thereof, and the prebiotic composition comprises the following raw materials in parts by weight: 35-45 parts of partially hydrolyzed complex gum, 30-40 parts of green banana resistant starch, 15-25 parts of enzymolysis okra polysaccharide and 3-5 parts of tea polyphenol. According to the composition, the partially hydrolyzed complex gum with specific molecular weight, the green banana resistant starch, the enzymolysis okra polysaccharide and the tea polyphenol are scientifically compounded, so that efficient proliferation of intestinal effective microbial communities and comprehensive and positive regulation and control of intestinal microenvironment are realized, and the composition is prepared by utilizing four components with complementary functions and different effects. The bacillus amyloliquefaciens can effectively proliferate traditional beneficial bacteria such as bifidobacteria and lactobacillus, can effectively nourish butyric acid producing bacteria such as clostridium praeparatum and inhibit growth of harmful bacteria, so that the bacillus amyloliquefaciens has the function of remarkably improving intestinal flora disorder caused by ampicillin, and has the functions of promoting generation of short-chain fatty acid and maintaining intestinal barriers.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention provides a method for deeply degrading penicillin V through combined circulating cascade of paracoccus and aspergillus niger and application of the method, and belongs to the technical field of antibiotic pollutant degradation, paracoccus is paracoccus sp KDSPL-02, aspergillus niger is Aspergillus niger, and the method is used for deeply mineralizing and degrading penicillin V to dissolve possible pollution caused by the paracoccus sp KDSPL-02 and the aspergillus niger is Aspergillus niger. Waste hyphae generated in the production process are subjected to harmless treatment.

The invention relates to a phenylacetylglutamine / phenylacetylglycine induced vascular smooth muscle cell senescence model and an establishment method and application thereof, and belongs to the technical field of cell models.The method comprises the following steps that vascular smooth muscle cell suspension is placed in a porous plate, 10% of FBS and 1% of mycillin are added into a culture medium, 24 h after cells adhere to the wall, the vascular smooth muscle cell senescence model is obtained, and the vascular smooth muscle cell senescence model is obtained. The method comprises the following steps of: adding a proper amount of phenylacetyl glutamine or phenylacetyl glycine dimethyl sulfoxide solution into a porous plate, changing a serum-free culture medium, continuously culturing for 24 hours, adding a proper amount of phenylacetyl glutamine or phenylacetyl glycine dimethyl sulfoxide solution into the porous plate, changing the culture medium added with phenylacetyl glutamine or phenylacetyl glycine once every 24 hours, and collecting cells after 72 hours. According to the invention, phenylacetylglutamine / phenylacetylglycine is used for inducing and establishing the vascular smooth muscle cell senescence model for the first time, the method is easy to operate and low in cost, and a foundation is laid for researching the relationship between cardiovascular diseases and senescence.

The present invention belongs to the technical field of semen cryopreservation, and more specifically, relates to sheep semen freezing solution and semen preservation method. The sheep semen freezing solution components of the present invention include Tris, citric acid, fructose, lactose, trehalose, penicillin, streptomycin, soy lecithin, nobiletin, glycerol and water. By using a specific content of nobiletin as an effective antioxidant component of a sheep sperm cryopreservative, it plays an antioxidant role when the sperm is frozen, making the sperm more stable during the cryopreservation process. After adding nobiletin, the vitality and motility of sheep sperm after freezing and thawing can be significantly improved. It effectively reduces the oxidative stress damage of sperm during the freezing process and improves the antioxidant capacity of sperm.

The invention relates to the field of medical liquid preparation, in particular to a medicine bottle shaking-up device for full-automatic high-speed liquid preparation equipment and the liquid preparation equipment. The medicine bottle shaking-up device comprises a medicine bottle shaking-up device body and the liquid preparation equipment, and the medicine bottle shaking-up device body is provided with a translation guide rail and a clamping installation table connected to the translation guide rail in a sliding mode; the clamping mounting table is driven by a crank rocker mechanism to do translational reciprocating motion, a penicillin bottle clamping device and an ampoule bottle clamping device are arranged on the table, and stop stations for feeding a medicine bottle and injecting liquid medicine are arranged in the motion stroke; the liquid dispensing equipment comprises a rack, a medicine bottle library, a liquid bag library, a needle cylinder library and the like, and the medicine dispensing process is completed through manipulators and equipment. According to the device, liquid medicine in an ampoule bottle is automatically sucked and injected into medicine powder in a penicillin bottle, then the crank and rocker mechanism does linear reciprocating motion to achieve mixing and uniform shaking of the medicine powder and the liquid medicine in the penicillin bottle, manual uniform shaking is replaced, and technical support is provided for batch, efficient and automatic medicine dispensing.

The utility model discloses a storage device for prolonging the shelf life of penicillin enzyme preparations, which comprises a storage box, the upper surface of the storage box is rotatably connected with a box cover through a hinge, the side inner wall of the storage box is fixedly connected with a partition plate, the side inner wall of the storage box is provided with a storage rack, and the upper surface of the storage rack is provided with a placing opening. The upper portion of the storage box is rotationally connected with a cover plate through a hinge, the lower bottom wall of the storage box is fixedly connected with a refrigerator, the output end of the refrigerator is fixedly connected with a conveying pipe, the lower surface of the storage box is fixedly connected with a motor, the output end of the motor is fixedly connected with a rotating shaft, and the end, away from the motor, of the rotating shaft is fixedly connected with fan blades. The reagent storage rack is simple in structure and convenient to operate, interference to reagents on other storage racks is reduced, the risk of reagent cross contamination caused by misoperation is reduced, reagent storage safety can be improved, and meanwhile reagent management orderliness is enhanced.

The invention discloses a culture medium for preparing a human-derived Merkel cell model and a construction method, and relates to the technical field of biologication.The key points of the technical scheme are that the culture medium comprises a culture medium E, and the culture medium E is formed by adding PRCi into an OMM culture medium; the OMM is prepared by adding GlutaMax (GlutaMax), B-27 (B-27) Minus, N2 (N2) suppliers, Penicilin-Streptomycin and 2-Mercaptoethanol into an Advanced DMEM (Dulbecco's Modified Eagle Medium) / F12 (Fullcap 12) culture medium and a Neurobasic culture medium, and the OMM is prepared by adding GlutaMax, B-27 Minus, N2 (N2) suppliers, Penicilin-Streptomycin and 2-Mercaptoethanol into a medium; and the PRCi comprises EED226, PRT4165 and GSK126 (glutamine synthase 126). According to the invention, a small molecule compound combination PRCi (EED226 + PRT4165 + GSK126) is added into a culture medium, so that the amount of MC in the organoid can be remarkably increased, and a human-derived system is established for researching the MC.

The present invention discloses an IS26-based resistance gene control plasmid, engineering bacteria, bacterial agent and application, belonging to the technical field of genetic engineering. The nucleotide sequence of the IS26-based resistance gene control plasmid is shown in SEQ ID NO: 1 or SEQ ID NO: 2. The engineering bacteria and bacterial agent containing the resistance gene control plasmid can be used for the control of resistance genes. By specifically destroying the insertion sequence IS26, the number of resistance genes can be fundamentally reduced and the spread of resistance genes can be blocked, providing a precise solution for the treatment of drug resistance. It has broad-spectrum resistance bacteria control performance and can effectively control gentamicin, tetracycline, chloramphenicol and ampicillin-resistant bacteria in wastewater, with the highest control efficiency reaching 83.76%. The plasmid, engineering bacteria and bacterial agent have low preparation costs, can be used for in-situ treatment of wastewater, have low treatment costs and good effects, and have broad application prospects.

The invention belongs to the technical field of product separation in enzymatic synthesis of antibiotics, and particularly relates to a method for separating and purifying amoxicillin and phenylacetic acid in the production process of amoxicillin. An immobilized penicillin acylase mutant is used for catalyzing penicillin potassium to synthesize amoxicillin in one step, a set of method for separating amoxicillin and phenylacetic acid is developed for an obtained reaction mixture, and the technical scheme mainly comprises the following steps: firstly, separating the immobilized penicillin acylase mutant from a reaction solution through filtration; then, amoxicillin is separated out through crystallization; and then crystallizing, separating and recovering phenylacetic acid through toluene extraction, back extraction and the like. According to the separation method, the amoxicillin can be rapidly and efficiently separated, the yield of the amoxicillin is relatively high, and the average crystallization rate reaches 93.22%; meanwhile, the separation and recovery effects of phenylacetic acid are good; and the extraction agent toluene can be recycled.

The present application relates to the field of biotechnology, in particular to a carbapenem-resistant escherichia coli phage and application thereof.A new escherichia coli phage vB_EOP_Se009 is isolated and purified, and the phage has good sterilization effect on carbapenem-resistant escherichia coli.The phage has strong tolerance to temperature and pH, is high in specificity and stability, can effectively solve the problem that carbapenem-resistant escherichia coli is resistant to multiple antibiotics, and provides an experimental basis for developing a preparation for preventing or treating carbapenem-resistant escherichia coli infection in clinic, and has great clinical application potential.

This invention provides the medical use of tyrosol in the preparation of formulations that reduce the toxicity of penicillin-thiazolic acid (PTA). It discloses a novel medical use of tyrosol in preparing formulations that reduce PTA toxicity. By using tyrosol to prevent and treat the residual toxicity of PTA, unnecessary antibiotic replacement regimens can be avoided, significantly reducing the economic burden. A method for preventing and treating PTA residual toxicity is provided, which can significantly reduce the toxicity of PTA and has good preventive and therapeutic effects. The prevention and treatment of PTA residual toxicity has significant socio-economic implications for controlling the hygiene and quality of animal-derived foods, protecting consumer health, and ensuring industrial production.

The utility model relates to the related technical field of penicillin bottles, in particular to a penicillin bottle sterilizing device which comprises a sterilizing box, a placing box is connected to the inner wall of the sterilizing box in a sliding mode, a fixing assembly is arranged on the inner wall of the placing box, and a water collecting assembly is arranged at the bottom of the placing box. Through the arrangement of the fixing assembly, the size of the space formed by the mounting plate and the opening of the movable plate can be adjusted by pulling the movable handle at the top of the movable plate, the opening in one side of the mounting plate is semicircular, and the movable plate is matched with the mounting plate; the first rubber block and the second rubber block at the opening are used for clamping the circular outer wall of the penicillin bottle through the relative movement of the first rubber block and the second rubber block at the opening, so that the penicillin bottle is fixed, the penicillin bottle is prevented from being collided and damaged in the sterilization process, the device can adapt to penicillin bottles with different outer diameter specifications, and in the penicillin bottle sterilization process, high-temperature steam can generate condensed water when encountering cold, so that the safety of the penicillin bottle is improved. Through the arrangement of the water collecting assembly, condensed water can be conveniently cleaned after sterilization is finished, and the condensed water is prevented from being accumulated in the device.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

This invention provides a nitrogen filling needle structure for vials, comprising a filling needle body, a first through hole penetrating its upper and lower end faces, and a filling needle body gas chamber inside the filling needle body; a top connector communicating with the first through hole at the upper end of the filling needle body; a second through hole penetrating its bottom end face at the bottom of the filling needle body, communicating with the filling needle body gas chamber; a first vent hole on the filling needle body, through which high-pressure gas passes sequentially via the top connector, the first through hole, the second through hole, and the first vent hole; a cap is fixedly installed on the bottom end face of the filling needle body at the top, with an opening at the bottom of the cap, and an annular rubber ring is provided on the inner wall of the cap, the annular rubber ring communicating with the interior of the filling needle body. This invention provides a nitrogen filling needle structure for vials, which can effectively prevent contamination of the liquid inside the vial during nitrogen filling, thereby improving the yield of finished products.

This invention discloses a method for isolating and extracting *Havnia vesicularis* using penicillin and vancomycin. The method mainly includes the following steps: inclusion and screening of patients with bipolar disorder; collection and processing of feces from these patients; isolation and treatment of *Havnia vesicularis* by adding penicillin and vancomycin to the feces; and identification of *Havnia vesicularis*. This invention improves the isolation efficiency and success rate of *Havnia vesicularis*, solving the problems of low isolation efficiency and poor identification accuracy in existing technologies.

The invention discloses an amplification culture solution of TILs cells and a culture method thereof. The amplification culture solution comprises a complete culture medium, and an anti-BTLA antibody with the final concentration of 0.1-10 [mu] g / mL, penicillin with the final concentration of 50-500 [mu] g / mL, streptomycin with the final concentration of 50-500 [mu] g / mL, gentamicin with the final concentration of 10-100 [mu] g / mL and amphotericin with the final concentration of 1-50 [mu] g / mL are added into the complete culture medium. The anti-BTLA antibody component in the culture solution can relieve inhibition of BTLA on T cells by blocking combination of BTLA and a ligand HVEM of BTLA, so that the anti-tumor capability of TILs is enhanced; meanwhile, after the BTLA is combined with the ligand HVEM, T cell activation and proliferation can be inhibited, and rapid proliferation of TILs is promoted; the irradiation PBMC added in the TILs cell culture process can provide an activation signal for the TILs and drive rapid proliferation of the TILs cells.

The invention relates to the field of automatic operation of dispensing equipment, in particular to a penicillin bottle uncapping method and system for an automatic dispensing machine, and the method comprises the steps: firstly setting a mechanical arm movement XYZ three-dimensional space coordinate system, calibrating a reference coordinate, and setting a slope and an intercept calculation target value in a segmented manner according to the diameter of a bottle body; the bottle body diameter and the bottle body total height of the penicillin bottle are obtained through visual recognition, a compensation value is calculated, the actual in-place coordinate point of the center position of the front-end clamping jaw is corrected, and the mechanical arm pose parameters and the actual in-place coordinate point are input into a mechanical arm control system to control the mechanical arm to execute uncapping operation; the uncovering system comprises a visual identification module, an uncovering mechanism, a mechanical arm control system, a front-end clamping jaw and the like. The technical effects that the position of the mechanical arm can be accurately corrected according to the diameters of different bottle bodies, and precise uncovering operation on the penicillin bottles is achieved are achieved.

The invention relates to the technical field of medicine packaging, and provides an inner cap of a penicillin bottle, the inner cap comprises a main body and a toggle clip part, the main body is provided with a groove for accommodating a rubber plug, and the bottom wall of the groove is provided with a through hole; one end of the toggle clip part is arranged on the inner wall of the groove, and the other end of the toggle clip part extends towards the bottom wall of the groove. The invention further provides a penicillin bottle cap combination which comprises an inner cap, an outer cap sleeve and a top cap, the inner cap can be inserted into the outer cap sleeve, an opening is formed in the upper end face of the outer cap sleeve, and an insertion opening communicated with the opening is formed in the side wall of the outer cap sleeve; a positioning piece capable of being inserted into the outer cover sleeve is arranged at the lower end of the top cover, and the top cover can seal the opening after the positioning piece is inserted into the outer cover sleeve. And the top cover can be deformed and separated from the opening after being subjected to external force away from the outer cover sleeve. By means of the technical scheme, the technical problem that in the prior art, a traditional tamper-proof bottle cap is not easy to open is solved.

The invention relates to the technical field of Escherichia coli detection, and particularly discloses a semisolid culture medium for rapid detection of Escherichia coli and a detection method and application thereof, the semisolid culture medium comprises the following components by weight: 4.2-5.8 g / L of agar; 8.5 to 11.5 g / L of tryptone; the semi-solid culture medium comprises the following components: 3.2 to 4.8 g / L of beef extract, 6.5 to 9.5 g / L of lactose, 1.2 to 2.2 g / L of D-glucose, 4.2 to 5.8 g / L of sodium chloride, 42000 to 58000 [mu] g / L of penicillin, 0.12 to 0.28 g / L of cholate, 0.06 to 0.14 g / L of disodium EDTA (Ethylene Diamine Tetraacetic Acid), 0.12 to 0.22 g / L of L-cysteine, 1.2 to 2.8 g / L of yeast extract, 0.005 to 0.015 g / L of vitamin B and an acid-base indicator, the semisolid culture medium and the detection method thereof provided by the invention not only solve the core pain point of the existing escherichia coli detection method in the technical level, but also reduce the operation cost and improve the benefit for enterprises in the economic level, promote the standardization and high efficiency of the food microorganism detection technology in the industry level, and have practicability and industrial popularization value.

The application provides a penicillin bottle image splicing method based on cylindrical correction and weighted fusion, and relates to the field of image processing. The penicillin bottle image splicing method based on cylindrical correction and weighted fusion comprises the following steps: collecting a cylindrical label image; respectively performing distortion correction on the cylindrical label image from horizontal and vertical directions to obtain an approximately planar label image; roughly estimating an image overlapping area, then performing image registration on the estimated overlapping area; performing feature extraction on the overlapping area image, performing coarse feature matching, removing mis-matching points, and finally realizing feature matching of the image according to the sorting of the ratio of the Euclidean distance of the nearest neighbor and the second nearest neighbor of the feature points; and performing image fusion on the image after feature matching. The method solves the problem of the traditional fade-in and fade-out fusion algorithm, which adopts linear weighted fusion, and the splicing line after image fusion is obvious, and ghosting phenomenon is prone to occur.

The utility model provides a penicillin bottle capping machine capable of automatically arranging caps, which relates to the field of capping machines and comprises a rack, a feeding conveying belt is arranged at one end of the rack, a discharging rail is arranged at the other end of the rack, a shifting wheel component is mounted and connected on the rack, a capping component is mounted and connected at one end of the discharging rail, and the other end of the capping component is connected with a lifting mechanism. The rack is provided and connected with a cap sorter; and the auxiliary conveying mechanism comprises a side frame, the side frame is fixedly connected with the rack, and protruding plates are arranged at the two ends of the side frame. The driving shaft rotates to drive the front end of the T-shaped block to drive the lifting seat to move upwards, so that bottle caps in the feeding hopper are automatically lifted to move upwards to the height of the cap sorter, automatic feeding of the bottle caps is conveniently completed, and feeding efficiency is improved; the winding roller rotates to enable the wound steel rope to be released stably, the locking position of the bolt lug is relieved, the feeding hopper rotates stably until the feeding hopper is in a toppling state, and it is ensured that bottle caps can be poured into the cap arranging device automatically.