The invention discloses a tissue sample preservative solution used for preserving tissue samples, such as fat, umbilical cords, placentas and the like, after collection and before separation. The tissue sample preservative solution mainly comprises following components: high-glucose DMEM dry-powder culture medium, sodium bicarbonate, DMSO, dexamethasone, insulin, penicillin, streptomycin, amphotericin and a heparin sodium injection. The high-glucose DMEM dry-powder culture medium and the sodium bicarbonate are used for maintaining osmotic equilibrium among cells inside and outside tissue, maintaining a pH value, maintaining a moistening situation of the tissue and providing nutritional components. The DMSO is a freeze-storage protective agent and can prevent freeze-injuries on the tissue samples. The dexamethasone can inhibit immunization and protect activity of stem cells in the tissue sample; the insulin can promote absorption and utilization of the tissue sample to glucose. The penicillin, the streptomycin and the amphotericin can prevent pollution from bacteria and moulds and can eliminate pollution which has occurred. The heparin sodium injection can prevent blood solidification in the tissue samples and increase a yield of the stem cells. The tissue sample preservative solution is simple in components, is low in cost, is convenient to use, can maintain activities of the stem cells in the tissue samples, such as fat, umbilical cords, placentas and the like, and can greatly reduce a time limit from collection to preparation of the tissue samples.