33 results about "Noggin" patented technology

The invention discloses a culture medium of a lacrimal gland organ and a culture method of the lacrimal gland organ, and the culture medium of the lacrimal gland organ is composed of a basic culture medium, R-spodin3, Noggin, B27, N-acetyl-L-cysteine, trehalose, vitamin C, glutathione, an adenylate cyclase activator, A8301, PGE2 and FGF10. The culture medium for lacrimal gland organ culture, provided by the invention, is prepared from various cell factor components according to an optimized proportion, and is simple and reasonable in components and rich in nutrition; when the culture medium is used for culturing the lacrimal gland organoid, the formation and growth of the organoid can be promoted, an in-vivo complex microenvironment is further simulated, the organoid is closer to an in-vivo physiological state, an organoid model is better constructed so as to evaluate the influence of drugs on lacrimal gland functions, and a new platform is provided for drug screening; and a new way is opened up for follow-up treatment of diseases such as xerophthalmia in the future.

The present invention relates to a linker compound having a fused nitrogen-containing heteroaromatic ring and a vinyl substituent for conjugating to a protein or peptide. The present invention further relates to a conjugate comprising the linker and an active agent and to a conjugate comprising the linker, a protein or peptide, and an active agent, such as a drug or labelling moiety. More specifically, the conjugate may be an antibody conjugate, such as an antibody drug conjugate (ADC).

The invention provides an organoid culture medium, an organoid culture method and application of the organoid culture medium, the organoid culture medium comprises a first-stage culture medium, the first-stage culture medium comprises a first-stage basic culture medium and first-stage addition factors, and the first-stage addition factors comprise N-acetylcysteine, nicotinamide, a ROCK inhibitor, Noggin, R-spondin, EGF, HGF, Gastrin, FGF10, Blebbistatin, Forskolin, A83-01, LDN193189, DAPT, TGF-alpha and UDAC. By using the organoid culture medium, the liver organoid and the fatty liver organoid can be effectively obtained, and the obtained fatty liver organoid has obvious fat deposition, is sensitive to fatty liver treatment drugs, highly restores the physiological status when human body fatty liver diseases occur, and has high application value.

The invention relates to a serum-free culture medium with limited chemical components for mesenchymal stem cells as well as a preparation method and application of the serum-free culture medium. The culture medium comprises a basic culture medium, growth factors and a carrier protein stabilizer, the growth factors comprise a proliferation promoting factor, an activating agent, a signal factor and nutrients; the activating agent comprises Wnt7a protein and LIF protein. According to the invention, Wnt7a, LIF, Noggin, Activin A and TGF-beta3 are combined together according to a specific concentration and proportion (especially Wnt7a and LIF) for the first time, and cooperate with other factors (FGF-2 and IGF-1) to construct a brand new signal network capable of simulating an in-vivo dry microenvironment.

A culture medium and a culture method for constructing prostate cancer organoids are provided. The culture medium includes a basic culture medium, and the following components with final concentrations: R-spondin1 15-200 ng / mL, Noggin 20-150 ng / mL, FGF2 5-15 ng / mL, FGF10 1-10 ng / mL, CHIR99021 0.5-1 μM, ALK inhibitor 0.25-1 μM, ROCK inhibitor 5-30 μM, nicotinamide 5-10 mM and sodium pyruvate 0.5-3 mM. The present culture medium and culture method can effectively improve the survival rate and formation efficiency of organoids through the compounding of each component.

The invention discloses an application of a protease cleavage site mutant of a natural immune signal linker protein MAVS in resisting African swine fever viruses, aiming at three sites of African swine fever protease pS273R cleavage MAVS, the research finds that the site mutant can efficiently inhibit the replication of the African swine fever viruses; and breeding the variety pigs capable of resisting African swine fever by utilizing a gene editing technology.

This invention discloses a crayfish head protein peptide with uric acid-lowering effect and its application, belonging to the field of biotechnology. The amino acid sequence of the crayfish head protein peptide is shown in any one of SEQ ID NO. 1-4. This invention uses ultrasonic and microwave technology to assist protein hydrolysis, prepares xanthine oxidase inhibitory peptides from crayfish heads, explores the material basis of its activity, and studies its mechanism of action, laying a theoretical foundation for the high-value utilization of crayfish processing by-products and the development of food-derived xanthine oxidase inhibitors.

This invention discloses a degradation-targeting chimera, nanomedicine, and composition of an anti-PRRSV biotype protein, and their applications, belonging to the field of bioengineering technology. Specifically, this invention discloses a degradation-targeting chimera of an anti-PRRSV biotype protein, wherein the degradation-targeting chimera is obtained by linking a PRRSV N protein-specific nanobody to the BTB domain of the E3 ubiquitin ligase adaptor protein SPOP via Nnb-SPOP. This lays a research foundation for the efficient in vivo delivery of novel biological drugs and their clinical application against PRRSV, and provides a new strategy for the comprehensive prevention and eradication of PRRSV in my country.

The application discloses a culture medium for constructing a gastric cancer ovary metastasis microtumor model and application. The application relates to the technical field of biotechnology, and provides a culture medium for constructing a gastric cancer ovary metastasis microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant protein EGF, bFGF, HGF, FGF-10, Wnt-3a, Noggin, R-spondin 1, Follistatin, CHIR99021, SB202190, A83-01, Primocin, N-acetyl-L-cysteine, nicotinamide, N2 additives, cholera toxin, B27, ITS-X, Y-27632, gastrin, prostaglandin E2, beta-estradiol, progesterone, Galunisertib and a basic culture medium. The culture success rate of the gastric cancer ovary metastasis microtumor model is effectively improved.

The invention relates to a liver cancer organoid culture medium and application thereof, and belongs to the technical field of medicines. The liver cancer organ-like culture medium disclosed by the invention is prepared from the following components: AdvDMEM F12, HEPES, GlutaMAX, B27, EGF (Epidermal Growth Factor), N-acetyl-L-cysteine, gastrin, HGF (Hepatocyte Growth Factor), FGF-10 (Fibroblast Growth Factor), A83-01, nicotinamide, N2, an adenylate cyclase activator, a three-antibody antibiotic, R-spondin1, Wnt-3a, Y-2763, Noggin, oncostatin M and FGF-23. The culture medium is rich in nutrition and reasonable in component; the liver cancer organoid cell spheres cultured by using the culture medium can stably grow for a long time, continuously amplify and repeatedly cryopreserve. When the culture medium is used for preparing the liver cancer organ model, a foundation is laid for knowing the pathogenesis of liver cancer and screening drugs.

The invention relates to the technical field of organoid culture, and discloses an induction culture medium for culturing a pig lung organoid. Advanced DMEM / F12 is used as a basic culture solution of the culture medium; the invention relates to a basic fibroblast growth factor composition, which is prepared from the following added components with the final concentration: 1500ng / mL of R-Spondinin, 25ng / mL of basic fibroblast growth factor 7 (FGF7), 10100ng / mL of basic fibroblast growth factor, 100ng / mL of Noggin protein, 50ng / mL of epidermal growth factor, 50ng / mL of Wnt3a protein, 500nM of TGF-beta pathway inhibitor, 5mM of ROCK inhibitor, 500nM of p38MAPK inhibitor, 1X of B27 supplement, 1.25 mM of N-acetyl-L-cysteine, 10mM of nicotinamide, 1X of glutamine supplement GlutaMAX1X, 1X of HEPES buffer solution and 1X of broad-spectrum antibiotic (Primocin). According to the method disclosed by the invention, the lung organ with the complex multi-cell lineage is efficiently and stably constructed from the pig lung adult tissue by a one-step method. The obtained organoid is high in biomimetic property and good in stability, provides a tool platform for pig lung development research, disease model construction, drug toxicology testing and regenerative medicine research, and has great scientific research value and wide application prospects.

The invention provides a culture medium for maintaining expression of key receptors of breast cancer organs and a preparation method of the culture medium. The culture medium comprises the following components: a basic culture medium, 1.25 to 2 mM of N-acetyl-L-cysteine, 1 * of N2, 1 * of B27, 1 * of Glutamax, 10 mM of HEPES, 1 [mu] M of SB202190, 5 to 10 mM of nicotinamide, 0.5 to 1 [mu] M of A83-01, 5 [mu] M of Y27632, 0.1 to 1 [mu] M of Verteporfin, 10 [mu] M of Sovesudil, 50 to 100 ng / mL of R-Spondin, 250 ng / mL of Noggin, 5 ng / mL of EGF, 5 ng / mL of Neuregulin 1, 200 ng / mL of The culture medium for maintaining the expression of the key receptor in the breast cancer organoid realizes stable maintenance of the expression of the key receptor in the breast cancer organoid, and provides a more accurate and reliable organoid model for research and treatment of breast cancer.

A system is disclosed for de novo protein generation. The system receives a set of design condition(s) that specify target characteristics of a synthetic protein. The system defines a modular energy function as a composition of a diffusion energy component and one or more conditioner energy components. The system applies a diffusion model to determine a denoised protein backbone. In applying the diffusion model, in each sampling step: the system transforms one prior sampled state of the synthetic protein from unconstrained space into constrained space based on the one or more design conditions, denoises the prior sampled state in the constrained space, and samples a subsequent sampled stated by applying a gradient of the modular energy function to the denoised prior sampled state in the constrained space. The final sampled state is a denoised protein backbone for the synthetic protein that satisfies the set of design condition(s).

The invention discloses a culture system and a culture method for intestinal organs of euryhaline fish, and belongs to the technical field of bioengineering. The culture system is composed of a basic culture medium and a complete culture medium, wherein the basic culture medium is Advanced DMEM / F12, and is supplemented with a GlutaMax, Hepes and mycillin double-antibody solution; and on the basis of the complete culture medium, N-2, B-27, N-acetylcysteine, EGF (Epidermal Growth Factor), Noggin, R-Spondin 1 and other key cell factor combinations are further added. By optimizing the tissue pretreatment and digestion process, the efficient fish intestinal tract organoid culture and passage method is established, the organoid model with the crypt-villus-like structure, the cell heterogeneity and the intestinal tract barrier function is successfully constructed, and the model provides a highly bionic in-vitro research tool for fish physiology and toxicology research.

Disclosed are vaccine compositions, in particular, polyvalent icosahedral compositions for presentation of an HA1 influenza antigen. The disclosed compositions may contain an S particle comprising a norovirus (NoV) S domain and an HA1 influenza antigen, which may be linked via a linker protein domain operatively connected to the norovirus S domain and an influenza antigen. Fusion proteins for producing the vaccine compositions, and methods of using the disclosed vaccine composition are also provided.

This invention belongs to the field of regenerative medicine, specifically relating to a lacrimal gland organoid culture medium and a method for in vitro culture of lacrimal gland organoids. In one aspect, a lacrimal gland stem cell proliferation culture medium is provided, comprising: lacrimal gland stem cell basal culture medium, EGF, Wnt3a, FGF7, FGF10, RSP1, and Noggin; the lacrimal gland stem cell basal culture medium comprises: DMEM medium, F12 medium, L-glutamine, fetal bovine serum, hydrocortisone, cholera toxin, B27, penicillin, streptomycin, amphotericin B, and fasudil. In another aspect, a lacrimal gland stem cell differentiation culture medium is provided, comprising: lacrimal gland stem cell basal culture medium, EGF, Wnt3a, FGF7, FGF10, RSP1, Noggin, and BMP7. The lacrimal gland organoids constructed by this invention are larger in volume than those of existing technologies, possessing numerous branched ducts and vesicular acinar structures, extending radially and multidirectionally in a three-dimensional manner, forming a complex and regular three-dimensional tubular acinar network, and possessing secretory function.

A system is disclosed for de novo protein generation. The system receives a set of design condition(s) that specify target characteristics of a synthetic protein. The system defines a modular energy function as a composition of a diffusion energy component and one or more conditioner energy components. The system applies a diffusion model to determine a denoised protein backbone. In applying the diffusion model, in each sampling step: the system transforms one prior sampled state of the synthetic protein from unconstrained space into constrained space based on the one or more design conditions, denoises the prior sampled state in the constrained space, and samples a subsequent sampled stated by applying a gradient of the modular energy function to the denoised prior sampled state in the constrained space. The final sampled state is a denoised protein backbone for the synthetic protein that satisfies the set of design condition(s).

The invention discloses a degradation targeting chimera of anti-PRRSV (porcine reproductive and respiratory syndrome virus) biological protein, a nano-drug, a composition and application of the degradation targeting chimera, the nano-drug and the composition, and belongs to the technical field of bioengineering. The invention discloses a degradation targeting chimera of anti-PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) biological type protein. The degradation targeting chimera is prepared by mixing Nnb-SPOP (N-methylpyrrolidone) with SPOP; the Nnb-SPOP is obtained by connecting a PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) N protein specific nano antibody with a BTB structural domain in an E3 ubiquitin ligase linker protein SPOP. A research foundation is laid for the aspects of in-vivo efficient delivery of novel biological drugs, anti-PRRSV clinical application and the like, and a new strategy is provided for comprehensive prevention, control and purification of PRRSV in China.

The use of immune-tolerized fetal stem cell-derived extracellular vesicles as a pharmaceutical composition for hair loss treatment is disclosed. The pharmaceutical composition of immune-tolerized fetal stem cell-derived extracellular vesicles for hair loss treatment contains noggin, sclerostin domain-containing protein 1 (SOSTDC1), fibroblast growth factor-7 (FGF-7), and platelet-derived growth factor-alpha (PDGF-α) to promote the conversion of hair in the catagen phase into hair in the anagen phase.

The application provides application of a ClpS1 gene in creating a heat-resistant cyanobacterial strain, and belongs to the technical field of genetic engineering. The application identifies, for the first time, a core function of a ClpS1 adaptor protein in high-temperature stress resistance of Synechocystis, and finds that the ClpS1 adaptor protein, as a heat shock-induced protein, has a long-acting effect in delaying the process of cell resistance to high-temperature stress. A better engineered strain (ΔClpS1-OE) than a wild strain is obtained by constitutively overexpressing the ClpS1 protein in a ΔClpS1 mutant, so that a more excellent cyanobacterial engineered chassis cell is created for photosynthetic organism synthesis.

The invention discloses a procambarus clarkii head protein peptide with a uric acid reducing effect and application, and belongs to the technical field of biology. The amino acid sequence of the procambarus clarkii head protein peptide is as shown in any one of SEQ ID NO.1-4. Protein hydrolysis is assisted by an ultrasonic microwave technology, the xanthine oxidase inhibitory peptide is prepared by taking the crayfish heads as raw materials, the material basis of the activity of the xanthine oxidase inhibitory peptide is researched, and the action mechanism of the activity of the xanthine oxidase inhibitory peptide is researched; and a theoretical basis is laid for high-value utilization of crayfish processing byproducts and research and development of food-borne xanthine oxidase inhibitors.

The invention provides a cervical cancer organoid culture medium and a culture method, and relates to the field of organ culture. The culture medium disclosed by the invention comprises a basic culture medium, a 4-hydroxyethyl piperazine ethanesulfonic acid buffer solution HEPES, Glutamax, an epidermal growth factor EGF, a fibroblast growth factor FGF, a hepatocyte growth factor HGF, a Noggin factor, R-Spondin1 protein, a cysteine derivative, nicotinamide Nicotinamide, Wnt-surface, A83-01, an estrogen, forskolin, a p38MAPK inhibitor, a GSK-3beta inhibitor and 1% of antibiotics.

The invention relates to the field of biology, and provides a culture medium for cervical stomach type adenocarcinoma organs and application of the culture medium, the culture medium comprises a basic culture medium and an additive, and the additive comprises Neuregulin 1 and at least one of the following components: B27, Y-27632, estradiol, A83-01, EGF, Noggin, R-Spondin1, hydrocortisone, Forskolin and wnt3a. According to the present invention, with the organoid culture medium, the cervical stomach type adenocarcinoma organoid can be effectively obtained, and the cervical stomach type adenocarcinoma organoid can maintain the histomorphological characteristics of the original tumor tissue, can rapidly amplify and grow, and can be stably subjected to passage cryopreservation and resuscitation, such that the important research platform is provided for the personalized drug screening and treatment, and the application prospect is broad. The method has profound significance for scientific research and clinical application.

De novo protein design, the rational design of new proteins from scratch with specific functions and properties, is a major challenge in molecular biology. Recently, deep generative models have emerged as a novel, data-driven tool for protein engineering. However, current diffusion- and flow-based models generally synthesize only backbones without sequences or side chains, while protein language models often model sequences. The present disclosure provides a flow-based protein structure generation method that can be conditioned to a given folding class.

The use of a composition, which includes the following compounds: Activin A, BMP4, CHIR99, EGF, FGF 8, FGF 10, IGF1, LY3, Noggin, retinoic acid and Y27, for implementing a differentiation process, preferably in vitro or ex vivo, of an induced pluripotent stem cell or iPSc, into a functional thymic epithelial progenitor or TEP.

Cranial placodes are embryonic structures essential for sensory and endocrine organ development. The efficient derivation of cranial placodes from human pluripotent stem cells is disclosed where the timed removal of the BMP inhibitor Noggin, a component of the dual-SMAD inhibition strategy of neural induction, triggers placode induction at the expense of CNS fates. Further fate specification at the pre-placode stage enables the selective generation of placode-derived trigeminal ganglia capable of in vivo engraftment, mature lens fibers and anterior pituitary hormone-producing cells that upon transplantation produce hormones including, but not limited to, human growth hormone and adrenocortiocotropic hormone in vivo. Alternatively, anterior pituitary hormone-producing cells are generated in cell culture systems in vitro.

The invention belongs to the field of preparation of specific medicinal liquor, and particularly relates to specific medicinal liquor for treating pain, itching, hot and cough toxin diseases and a preparation method of the specific medicinal liquor. The preparation method comprises the following steps: grinding multifunctional mixed Chinese herbal medicines into powder, soaking Chinese herbal medicine powder with litsea cubeba oil and a calcium ion solution, and rapidly and fully crushing cell walls and cell membranes of Chinese herbal medicine plants with increased brittleness in cooperation with an ultrasonic crusher to release all effective components; the yellow wine can accelerate blood circulation, high-concentration alcohol rapidly penetrates through the medicinal materials under the action of magnetic force, the solubility is enhanced, components released from Chinese herbal medicine cells are fully dissolved, and small molecule peptides obtained by taro protein decomposed by papain are matched, so that the medicinal liquor does not deteriorate while containing a large number of effective components and being high in medicine effect, and the medicinal liquor has a good health care effect. The symptoms of dermatitis-eczema mice with pain, pruritus, hot skin and toxin-containing skin are effectively relieved, and the cough inhibition rate reaches 34.9%.

The application relates to the technical field of nanocomposites, in particular to MoS2-HA-ICG-cGAMP and a preparation method and application thereof, which comprises the following steps: mixing a liquid containing molybdenum disulfide with a liquid containing hyaluronic acid, stirring overnight, and preparing a MoS2-HA solution; adding an EDC solution to the MoS2-HA solution, activating, adding ICG drop by drop, then sealing with a sealing film, and shaking on a shaker to prepare a MoS2-HA-ICG solution; adding cGAMP to the MoS2-HA-ICG solution, stirring, and preparing a MoS2-HA-ICG-cGAMP solution. The application loads hyaluronic acid on MoS2 nanomaterials, can increase biological safety and stability, and can specifically recognize tumor cells; ICG and MoS2 nanomaterials have similar wavelengths, can be excited by the same wavelength laser, and can better penetrate deeper living tissues; cGAMP is loaded, cGAMP is delivered to adaptor protein stimulator of interferon genes (STING) to activate the innate immune system, the activated STING can be transferred between cells to promote anti-tumor immunity, trigger the production of type I interferon and proinflammatory cytokines, and thus start the immune response.

De novo protein design, the rational design of new proteins from scratch with specific functions and properties, is a grand challenge in molecular biology. Recently, deep generative models have emerged as a novel data-driven tool for protein engineering. However, current diffusion- and flow-based models generally synthesize backbones only, without sequence or side chains, while protein language models often model sequences instead. The present disclosure provides flow-based protein structure generation which can be conditioned on a given fold class.

This invention provides a method for culturing bronchial epithelial stem / progenitor cells. Specifically, this invention provides a culture medium for culturing bronchial epithelial stem / progenitor cells, comprising a basal culture medium and additives; wherein the basal culture medium is selected from the group consisting of DMEM, Ham's F-12, DMEM / F-12, or combinations thereof, and the additives include: fetal bovine serum, glucose, insulin, epidermal growth factor, hydrocortisone, Y-27632, SB431542, Noggin, and R-spondin. 1. Fibronectin and E-Cadherin are provided, along with a method for culturing bronchial epithelial stem / progenitor cells. The bronchial epithelial stem / progenitor cells amplified using the method of this invention have a high cloning rate, can be isolated from trace amounts of tissue, and can be passaged more than 50 times. The cells maintain normal stemness and differentiation potential, and can be cultured to obtain a sufficient number of clinically infused bronchial epithelial stem / progenitor cells for autologous infusion. Clinically, this has achieved the repair of small airway damage in the lungs and significantly improved lung function.