85 results about "Primordium" patented technology

The invention discloses a method for improving plant polyploidy induction rate and obtaining polyploidy based on leaf bud primordium cell cycle synchronization. The method comprises the following steps: carrying out pre-differentiation culture on an explant induction material; performing cell cycle synchronization excitation treatment on the leaf bud primordium cells obtained by the pre-differentiation treatment; and performing chromosome doubling treatment on the explant induction material subjected to the cell cycle synchronization excitation treatment. According to the method disclosed by the invention, an EdU staining labeling method and a flow cytometry are used for accurately judging the time for carrying out cell cycle synchronization excitation treatment on leaf bud primordium cells of an induction material, and carrying out synchronization excitation and doubling induction on the induction material in time; the dosage of a cell cycle inhibitor for synchronous excitation treatment is accurate, the treatment opportunity is accurate, the treatment duration is proper, and the polyploidy inductivity of the plant is remarkably improved; the obtained polyploidy plant is good in stability, and the method is high in repeatability.

The invention relates to an industrial cultivation method for Tremella aurantialba, and belongs to the technical field of biology. The method comprises the following steps: (1) preparing a Chinese tremella aurantialba cultivation bag; (2) feeding the Chinese tremella auramtialba cultivation bag into a cultivation room for cultivation; culturing until mycelia completely cover the culture material in the culture bag; (3) continuously culturing until the primordium is formed; and (4) after the primordium is formed, putting the cultivation bag of the Tremella aurantialba into a fruiting room for culturing for 30-45 days until the sporocarp petals of the Tremella aurantialba are completely opened and are golden yellow, and then harvesting the Tremella aurantialba. Wherein a beauveria bassiana spore suspension with the concentration of 0.1-0.5% is sprayed on the surface of the tremella auramtialba cultivation bag for 1-3 times during the cultivation period. When the cultivation method is used for cultivation, the obtained fruiting body dry goods are golden yellow in color and luster, the problem that the color and luster of the tremella aurantialba fruiting body dry goods obtained through a traditional cultivation mode are blackened is solved, and the product quality is improved.

The invention discloses an efficient sparassis crispa bottle cultivation method, and belongs to the technical field of edible fungus cultivation. The bottle cultivation method comprises the steps of cultivation raw material preparation, compost preparation, fruiting bottle production, inoculation, fungus bottle culture, primordium induction and sporocarp culture. Specific fermentation raw materials are adopted to replace or partially replace pine sawdust to serve as cultivation main materials, humic acid, citrulline and arabinogalactan serve as additives, and efficient production of sparassis crispa is achieved in a bottle cultivation mode. According to the cultivation method, the primordium is easy to form, the consistency is good, the operation is simple, the biological conversion rate can reach 65-70%, and the growth and development period is 75-90 days.

A cultivation method for Morchella on patterned layer frames is provided. The method includes: (1) preparing and laying a culture substrate, and sowing; (2) supplementing an exogenous nutrient material; (3) mycelium culture; (4) primordium induction; (5) primordium differentiation; (6) management of a young mushroom period; (7) maturity management; and (8) erchao mushroom management. The method adopts a low temperature of (5-9)° C. to promote a transformation of an exogenous nutrition and adopts a low temperature of (2-4)° C. to induce a differentiation of primordium and other measures, to address a bottleneck of industrial development that cannot continuously supply fresh Morchella to the market, which is caused by some problems in the cultivation of Morchella, such as an unstable yield and seasonal limitation in the existing cultivation of Morchella. The method can carry out industrial facility cultivation for Morchella, and can obtain Morchella with stable yield and good quality.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention discloses an edible fungus multi-parameter growth model construction and environment intelligent optimization method based on deep learning, and the method comprises the steps: collecting environment data, such as temperature, relative humidity, carbon dioxide concentration, illumination intensity, and matrix moisture content, and hypha and sporocarp images; the growth state index is constructed through weighted fusion of hypha respiration intensity, primordium density, pileus color gradient and other characteristics and is used for representing the growth health degree. And predicting a future growth trend based on an image and environment dual-channel feature fusion model, and obtaining prediction credibility according to a prediction error. An environment regulation and control strategy is established according to growth state improvement, energy consumption and physiological risks, equipment control instructions of ventilation, humidification, dehumidification, light supplement and the like are output, and dynamic optimization of a multi-parameter environment is achieved; and meanwhile, the model is updated online in combination with feedback data, so that the system has the capability of adapting to environment change and strain difference. The system can realize accurate monitoring and automatic regulation and control of the growth state of the edible mushrooms, and is suitable for industrialized production of the edible mushrooms.

The invention discloses a kudzu vine root seedling raising method. The disclosed method comprises the following steps: sequentially soaking kudzu vine root cuttings in a carbendazim solution and a growth solution; the growth solution is a mixed solution of growth hormone and an aqueous extract of fragrant eupatorium herb; the radix puerariae cuttage branches are lignified kudzu vines with bud nodes; performing cuttage on the soaked cuttings in a rooting medium for culture; the temperature of the culture environment is 26-30 DEG C, and the humidity is 76%-82%; when the cuttings are grown and buds grow to 5cm, applying a nutrient solution to the roots; and continuing to culture until the cutting wood roots and the root hair grows to 10cm to obtain the kudzu vine root seedlings. According to the method, the culture mode, the culture medium and the culture device are improved, so that the root primordium forming number of the cuttings is effectively increased, the rooting rate is increased, and the seedling raising period is shortened.

The invention relates to a cultivation method of morchella esculenta, and belongs to the technical field of industrialized production of edible mushrooms. The method comprises the steps of culture medium preparation and laying, sowing, exogenous nutrient supplement, mycelium culture, primordium induction, primordium differentiation, young mushroom and maturity management, second flush mushroom management and the like. Mountain immature soil, rice hull powder, chestnut sawdust and the like are selected as main materials of a used culture medium, peracetic acid fermentation treatment is combined, and traditional formaldehyde treatment which is high in cost and harmful to the environment is replaced. Fermentation type nutrition bricks are adopted as exogenous nutrition materials, and on-site preparation and sterilization operation are replaced. Natural light is combined with LED light supplement, temperature and humidity environments are regulated and controlled in a partitioned mode, energy consumption is reduced, and the thallus development stability is improved. Unified management and batch harvesting are achieved through a multi-layer frame and standard box modular structure. The method has the advantages of low production cost, simplicity in technical implementation, stable yield, suitability for large-scale popularization and the like.

The invention provides an open type tissue culture method for plica rugosa, which comprises the following steps: S1, performing disinfection treatment on a plica rugosa explant to obtain a pretreated explant; s2, the pretreated explant is inserted into a first modified MS culture medium for first-stage culture, and a first-stage explant is obtained; s3, transferring the first-stage explant into a second modified MS culture medium for second-stage culture to obtain a second-stage explant; s4, transferring the second-stage explant into a third modified MS culture medium for third-stage culture to obtain a primary seedling. According to the method, cell-free fermentation filtrate and PPM are sequentially and progressively added into a three-stage modified MS culture medium, so that high-efficiency culture of the explant of the plica rugosa in an open environment is realized, the pollution rate and the browning degree are reduced, meanwhile, healing of the explant, adventitious bud differentiation and root primordium formation are promoted, and the seedling rate of open tissue culture of the plica rugosa is increased.

The invention discloses a Sparassis sp. Strain (FJSXQJ-01) suitable for industrial cultivation, the preservation number of the strain is GDMCC 65276, the strain is preserved in Guangdong Microbiological Culture Collection Center, and the ITS sequence of the strain is as shown in SEQ ID NO: 1; the invention further discloses a cultivation method of the sparassis crispa strain. The cultivation method comprises the steps of mother strain preparation, stock culture, culture medium preparation and culture, primordium induction, fruiting management and the like. According to the sparassis crispa strain suitable for artificial cultivation, wild resource collection and systematic domestication breeding are carried out, the sparassis crispa variety suitable for artificial cultivation is bred, the sparassis crispa strain suitable for artificial factory cultivation and the matched cultivation method have the advantages that hyphae grow fast and are high in infectious microbe resistance, primordium is self-colored and robust, and the fruiting rate reaches up to 90% or above.

The invention discloses an intelligent identification and calculation method for growth phenotypic characteristics of edible fungi in a budding period, and relates to the technical field of modern facility agriculture. Comprising the following steps: shooting and obtaining a clear RGB image of field growth in the edible mushroom germination period; performing contrast enhancement processing on the clear RGB image; accurate identification is carried out through an improved instance segmentation model RSL-YOLOv8m-Seg to obtain a bud primordium mask image; traversing and segmenting each bud primordium mask one by one, and extracting the contour of the mask; reconstructing a bud primordium contour by adopting a contour fitting method based on each mask contour; extracting morphological characteristics of the bud primordium; and counting growth phenotypic characteristics in a budding period. According to the method, the growth characteristics of the edible fungi in the germination period can be accurately calculated, and a phenotypic characteristic basis is provided for intelligent and accurate management and control of an edible fungi house.

A cultivation method for improving the quality and yield of black fungus using hydrogen-rich water involves spraying a hydrogen-rich water composition three times during mycelial culture, spraying it every two days during primordium differentiation, and spraying it daily during fruiting body enlargement. The hydrogen-rich water composition comprises hydrogen-rich water, xylose, humic acid, gelatin, hydroxypropyl cellulose, and deionized water. This invention effectively solves the problems of poor hydrogen concentration stability and hydrogen volatilization in hydrogen-rich water by adding functional components such as gelatin, hydroxypropyl cellulose, xylose, and humic acid to the hydrogen-rich water, thus extending the time of hydrogen concentration stability and its effect. By using the above-mentioned hydrogen-rich water composition during the mycelial growth and primordium differentiation stages of black fungus, the microenvironment for black fungus growth is comprehensively optimized, resulting in increased yield, improved quality, and enhanced marketability. This provides an effective way to cultivate superior black fungus varieties.

[Problem] To provide a matsutake primordium having a high methyl cinnamate content and a method capable of obtaining such a matsutake primordium having a high methyl cinnamate content at an artificially high yield. [Solution] Cells having the ability to form a primordium that have been isolated from matsutake fruiting bodies are cultured in a medium containing a protein degradation product and having a specific phenylalanine concentration.

The present application relates to the technical field of genetic engineering, and particularly relates to a fruiting inhibition engineering bacterium and a construction method and application thereof, comprising overexpressing a transcription factor Pcc1 in a ganoderma strain, constructing an engineering bacterium MKLGE253241 with high expression of Pcc1, a preservation unit: China General Microbiological Culture Collection Center, address: No. 1, Xibei Road, No. 3, Chaoyang District, Beijing, preservation date: March 11, 2026, preservation number: CGMCC No. 42592. The present application blocks the formation of fruiting body primordium from the molecular level, maintains the mycelium in vegetative growth, and increases the average biomass by 15.2%, without fruiting body; meanwhile, the leather flatness of the mycelium produced by the engineering bacterium is significantly improved, and the scale qualified rate is obviously improved. The present application fundamentally solves the problem of fruiting body leather, avoids chemical inhibitors, and provides a new path for high-performance bio-based leather.

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a method for improving the fruiting high-quality rate of dictyophora rubrovolvata, which comprises the following steps: 1) sterilizing and insecticidal treatment is carried out on the inside and outside of an intelligent mushroom house, then subsoil and strains are laid after the intelligent mushroom house is closed for 24 hours, and then earthing is carried out; 2) after earthing, keeping the environment humidity to be 60-70%, the temperature to be 17-19 DEG C and the carbon dioxide concentration to be 4000-5000ppm, and culturing for 15-20 days; 3) keeping the environment humidity at 70-82%, the temperature at 19-21 DEG C and the carbon dioxide concentration at 3000-4100 ppm, and culturing for 15 days; 4) keeping the environment humidity to be 88-92%, the temperature to be 23-27 DEG C and the carbon dioxide concentration to be 1000-2000ppm, culturing for 10 days, spraying a nutrient solution, and continuously culturing for 45 days; (5) intensively picking the bamboo eggs with sharp tops and more than 80g per egg into a special container, and carrying out fruiting management; according to the method, the primordium forming number can be effectively controlled, so that the consistency of fruiting and production is effectively improved, the differentiation primordium can be promoted to quickly form bamboo eggs, the growth cycle is shortened, the high-quality rate of bamboo flowers is increased, and the yield of dictyophora rubrovolvata is effectively increased.

The present invention discloses a method for cultivating Morchella esculenta in saline-alkali land, belonging to the technical field of edible mushroom cultivation. The method includes steps of greenhouse ground arrangement, laying a salt-blocking and water-retaining layer, sowing, placing nutrient bags, spawn running, mushroom primordium induction and fruiting management. The present invention innovatively lays a crop straw layer, a fruit shell granule layer and a sawdust layer layer by layer in the soil layer of saline-alkali land in coastal areas. The three layers together form a salt-blocking and water-retaining layer, which can prevent the upward penetration of deep soil salts, reduce water evaporation, and maintain soil humidity; it can also ensure that the soil in the spawn layer is moist but not wet, prevent waterlogging in the spawn layer, and promote spawn running of the spawn; the present invention also fully regulates environmental factors such as bed surface temperature, soil moisture, air humidity, ventilation, light, etc. in the cultivation of Morchella esculenta in saline-alkali land. Each condition coordinates and cooperates to comprehensively improve the cultivation yield and quality of Morchella esculenta in saline-alkali land in coastal areas, providing a scientific theoretical basis for the development of the Morchella esculenta cultivation industry in saline-alkali land of coastal cities.

The present application belongs to the technical field of edible mushroom cultivation, and particularly relates to a method for cultivating Armillaria mellea fruiting bodies by using old Gastrodia elata culture material, wherein the cultivation process of Armillaria mellea is optimized, so that the Armillaria mellea primordium and fruiting body induction rate can be provided, a large amount of Armillaria mellea can be cultured, the old Gastrodia elata culture material can be reused, the resource waste can be reduced, the ecological environment can be effectively protected, the economic income of the grower can be improved, the industrial development enthusiasm can be enhanced, the high-quality development and sustainable development of the Gastrodia elata industry can be promoted, and the development and utilization of the Armillaria mellea resource are laid a foundation.

The invention provides a morchella sextelata strain pleurospermum hookeri No.8 and application thereof, and relates to the technical field of strain breeding, the morchella sextelata strain pleurospermum hookeri No.8 is obtained by space-carrying induced genetic variation, then subculture is carried out to screen out stably inherited characters, and the morchella sextelata strain pleurospermum hookeri No.8 is screened through a fruiting screening test. The pleurospermum hookeri No.8 strain has the characteristics of high hypha germination speed, low temperature resistance, white mold resistance (Diplospora longipora), early primordium generation, large quantity, strong cluster growth type of young mushrooms, good shape of commercial mushrooms, difficulty in breakage and the like, and has a wide application prospect.

The invention discloses a morchella hypha powder prefabrication method and equipment line, belongs to the technical field of morchella hyphae, and solves the problems that morchella is extremely sensitive to continuous cropping, metabolite changes the soil microenvironment, hypha division and nutrient absorption are directly hindered, the hypha growth speed is reduced by 30%-50%, the biomass is reduced, and primordium formation is delayed. Comprising the following steps: S1, carrying out stress-resistant intensified culture on liquid strains; chitosan in a specific proportion in a liquid strain neutralizes dopamine quinone, antioxidant enzyme is induced to be improved, fat-soluble toxin is embedded in beta-cyclodextrin in a solid culture medium, dendrobium nobile residues adsorb water-soluble toxin, sodium selenite enhances oxidation resistance, germanium dioxide promotes hypha branching, an overburden filtrate degrades dopamine quinone, ash hydrolyzes related toxin, and the yield is increased. Pseudomonas compensates for beneficial metabolites and accelerates metabolism through temperature difference stimulation, so that hypha growth is accelerated, differential metabolites are reduced, biomass is increased, and the three-crop fruiting rate is stable.

The utility model discloses an edible and medicinal fungus fruiting cover which comprises a sleeve cover capable of covering a bottle opening of a cultivation bottle in a clamping mode, a breathable piece covering the top of the sleeve cover and a waterproof cover coaxially assembled on the sleeve cover. The top of the sleeve cover is coaxially provided with an external thread sleeve for threaded connection of the cover body. The utility model relates to the technical field of edible mushroom planting. According to the edible and medicinal fungus fruiting cover, the necking opening of the conical surface structure is formed in the bottom of the sleeve cover, when the sleeve cover covers a bottle opening of a cultivation bottle, the necking opening can be directly inserted into a culture medium, and compared with a traditional mesh plate structure and a traditional annular necking opening structure, the edible and medicinal fungus fruiting cover is beneficial to gently guiding the growth direction of mushrooms; according to the edible and medicinal fungus culture medium, the probability of deformation or breakage of edible and medicinal fungus sporocarps in the growth process is reduced, the commodity and yield of the edible and medicinal fungus are improved, in addition, the detachable breathable sheet and the waterproof cover are matched, the sleeve cover does not need to be detached when the primordium is formed, and the primordium is prevented from being damaged.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

Systems and methods for generating a plurality of unique edits in a T1 seed of a plant are provided. In one example, a method includes transforming at least one expression cassette into a plant cell or plant tissue. The at least one expression cassette may comprise a nucleic acid encoding a DNA modifying enzyme; optionally, a nucleic acid encoding at least one guide RNA (gRNA); and a floral mosaic (FMOS) regulatory sequence wherein the FMOS regulatory sequence (i) mediates expression of the DNA modifying enzyme in at least one of a floral primordium cell and a floral reproductive organ, and (ii) mediates a plurality of edits in at least one of the floral primordium and the floral reproductive organ. The method may also include regenerating the plant cell or plant tissue into a TO plant having a plurality of T1 seeds, wherein the T1 seeds contain a plurality of unique edits.

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.

The invention discloses a hot-start high-specificity LAMP (loop-mediated isothermal amplification) isothermal nucleic acid rapid detection method which comprises the following operation steps: step 1, preparing three pairs of LAMP amplification primers, the 3'ends of one pair of primers F3 and B3 being one of ddC / ddG / ddT / ddA or other closed groups, and each of the primers F3 and B3 internally containing an RNA (ribonucleic acid) basic group; 2, establishing an LAMP isothermal nucleic acid amplification reaction system by using three pairs of primers; 3, in the process of hot start of the high-specificity LAMP amplification reaction, after the primers F3 and B3 are paired with the template DNA, cutting off the DNA at the RNA base by thermal stability endonuclease HII to generate a double-dumbbell initiator (a product 6 in the figure 1), thereby starting the LAMP continuous amplification reaction; if the primers F3 and B3 cannot be specifically paired with the DNA template, the primers F3 and B3 cannot be cut by thermal stability endonuclease HII, and a double-dumbbell type initiator cannot be generated, so that the LAMP continuous amplification reaction cannot be started.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

The invention discloses a method for breeding by culturing sporocarp under pure culture conditions of boletus sinensis, and belongs to the technical field of edible mushroom breeding and cultivation. The method comprises the following steps: activating the boletus sinensis strain JSJ-Bx1, inoculating the boletus sinensis strain JSJ-Bx1 into a culture medium for dark culture, then performing low-temperature induction culture on an activated flat plate which is nearly overgrown with hyphae, changing the hyphae from white to golden yellow and generating hypha kinking, then transferring the hypha flat plate into a fruiting room for culture, and after obvious primordium differentiation is seen, inoculating the hyphae into a culture medium for culture, thereby obtaining the boletus sinensis strain JSJ-Bx1. Continuously performing induced culture to develop into mature sporocarp; sporocarp basidiospores are collected, diluted and coated in a B1 culture medium for germination culture, and monospora hyphae and polyspora hyphae are obtained and serve as stock seeds for breeding the boletus sinensis. According to the method, the mature sporocarp of the boletus sinensis can be directly and rapidly cultured in the agar culture medium, the harvested basidiospore can normally germinate and grow, and the whole life history of the boletus sinensis can be completed under the pure culture condition.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

The invention discloses an intelligent cultivation method for phellinus igniarius based on environmental parameter regulation and control, and particularly relates to the cross technical field of intelligent agriculture and fungus cultivation, and the method comprises the following steps: constructing a phellinus igniarius growth prediction model; respectively setting differentiated target environment parameter range sets for a mycelium development period, a primordium formation period and a sporocarp growth period; in the cultivation process, environmental data are monitored in real time and input into the model to obtain a predicted growth state, and a regulation and control instruction is generated by comparing the predicted growth state with an expected state, so that environmental parameters are maintained in a target range; when the mycelium reaches physiological maturity, a sporocarp differentiation program is automatically triggered, and an environment target is switched; in the later growth period of the sporocarp, morphological characteristics are analyzed through images and compared with standard morphological characteristics, if the deviation exceeds a threshold value, a parameter optimization instruction is generated, and an environment parameter set for optimizing the growth period of the sporocarp is fed back. Precision, automation and intelligence of phellinus igniarius cultivation are achieved, and the yield and quality are improved.

The invention provides a gene for regulating and controlling differentiation of a plant spikelet primordium to seta and application, and belongs to the technical field of molecular biology. The invention provides a protein for regulating and controlling differentiation of a plant spikelet primordium to seta. The protein comprises an amino acid sequence as shown in SEQ ID No. 1. The invention also provides a gene sequence for coding the protein, and a biological material for regulating and controlling the differentiation of the plant spikelet primordium to the seta is prepared on the basis of the gene sequence or the protein, and the transformation of the plant seta to the grain can be regulated and controlled by utilizing the biological material. After the coding gene of the protein is knocked out, differentiation of a part of spikelet primordia to seta can be successfully inhibited, and the part of spikelet primordia which are not differentiated into seta is differentiated into spikelet with stems. The protein and the gene are excavated, so that the molecular mechanism of seta development of the millet can be filled, and meanwhile, the high-yield breeding process of the millet can be promoted.

The invention discloses a fungus expression vector containing a green fluorescent protein reporter gene as well as a construction method and application of the fungus expression vector, and belongs to the technical field of edible fungus genetic engineering. On the basis of flammulina velutipes strain 0747 transcriptome data, a promoter sequence of a gene hyd9 efficiently expressed in hyphae is obtained through screening and cloning, and the promoter sequence has conserved structures such as a TATA box and a CAAT box. The expression activity of the promoter in hyphae is about 60 times that of an Ffgpd promoter. By constructing a hyd9-driven egfp expression vector and converting flammulina velutipes, the result shows that a transformant presents strong green fluorescence, the egfp transcriptional level is significantly higher than that of a Glgpd and Ffgpd promoter control group, a fluorescence signal is weakened in primordium and sporocarp but still can be detected, the mode is consistent with an endogenous hyd9'hypha high expression-sporocarp low expression 'mode, and the expression of the endogenous hyd9-sporocarp low expression is consistent with that of the endogenous hyd9. The promoter is proved to be capable of driving the expression of exogenous genes in a space-time specific manner.