62 results about "Primordium" patented technology

A cultivation method for Morchella on patterned layer frames is provided. The method includes: (1) preparing and laying a culture substrate, and sowing; (2) supplementing an exogenous nutrient material; (3) mycelium culture; (4) primordium induction; (5) primordium differentiation; (6) management of a young mushroom period; (7) maturity management; and (8) erchao mushroom management. The method adopts a low temperature of (5-9)° C. to promote a transformation of an exogenous nutrition and adopts a low temperature of (2-4)° C. to induce a differentiation of primordium and other measures, to address a bottleneck of industrial development that cannot continuously supply fresh Morchella to the market, which is caused by some problems in the cultivation of Morchella, such as an unstable yield and seasonal limitation in the existing cultivation of Morchella. The method can carry out industrial facility cultivation for Morchella, and can obtain Morchella with stable yield and good quality.

The invention discloses a method for separating and transplanting spermatogonial stem cells of Chinese sturgeons, and belongs to the technical field of separation of germline stem cells of fishes. The separation method comprises the following steps: washing Chinese sturgeon testis tissues with an L-15 culture medium containing penicillin / streptomycin double antibodies, and carrying out physical shearing, digestive enzyme enzymolysis and screen filtration to obtain a Chinese sturgeon testis cell suspension; performing percoll density gradient centrifugation to obtain high-purity spermatogonial stem cells of the Chinese sturgeons; and dyeing, washing, resuspending and carrying out micro-injection transplantation to enter the gonad primordium reproductive ridge part of the juvenile acceptor fish. According to the physical characteristics of the spermatogonial stem cells of the Chinese sturgeons, the spermatogonial stem cells with high purity are obtained by adopting percoll density gradient centrifugation, and the purpose of abdomen borrowing reproduction is achieved by adopting a microinjection mode, so that the preservation of germ cells of endangered fishes and the reconstruction of artificial populations are realized; and a new technical approach is provided for protecting genetic resources of the Chinese sturgeons and breaking through artificial propagation bottlenecks.

The invention discloses an edible fungus multi-parameter growth model construction and environment intelligent optimization method based on deep learning, and the method comprises the steps: collecting environment data, such as temperature, relative humidity, carbon dioxide concentration, illumination intensity, and matrix moisture content, and hypha and sporocarp images; the growth state index is constructed through weighted fusion of hypha respiration intensity, primordium density, pileus color gradient and other characteristics and is used for representing the growth health degree. And predicting a future growth trend based on an image and environment dual-channel feature fusion model, and obtaining prediction credibility according to a prediction error. An environment regulation and control strategy is established according to growth state improvement, energy consumption and physiological risks, equipment control instructions of ventilation, humidification, dehumidification, light supplement and the like are output, and dynamic optimization of a multi-parameter environment is achieved; and meanwhile, the model is updated online in combination with feedback data, so that the system has the capability of adapting to environment change and strain difference. The system can realize accurate monitoring and automatic regulation and control of the growth state of the edible mushrooms, and is suitable for industrialized production of the edible mushrooms.

The invention relates to a cultivation method of morchella esculenta, and belongs to the technical field of industrialized production of edible mushrooms. The method comprises the steps of culture medium preparation and laying, sowing, exogenous nutrient supplement, mycelium culture, primordium induction, primordium differentiation, young mushroom and maturity management, second flush mushroom management and the like. Mountain immature soil, rice hull powder, chestnut sawdust and the like are selected as main materials of a used culture medium, peracetic acid fermentation treatment is combined, and traditional formaldehyde treatment which is high in cost and harmful to the environment is replaced. Fermentation type nutrition bricks are adopted as exogenous nutrition materials, and on-site preparation and sterilization operation are replaced. Natural light is combined with LED light supplement, temperature and humidity environments are regulated and controlled in a partitioned mode, energy consumption is reduced, and the thallus development stability is improved. Unified management and batch harvesting are achieved through a multi-layer frame and standard box modular structure. The method has the advantages of low production cost, simplicity in technical implementation, stable yield, suitability for large-scale popularization and the like.

The invention provides an open type tissue culture method for plica rugosa, which comprises the following steps: S1, performing disinfection treatment on a plica rugosa explant to obtain a pretreated explant; s2, the pretreated explant is inserted into a first modified MS culture medium for first-stage culture, and a first-stage explant is obtained; s3, transferring the first-stage explant into a second modified MS culture medium for second-stage culture to obtain a second-stage explant; s4, transferring the second-stage explant into a third modified MS culture medium for third-stage culture to obtain a primary seedling. According to the method, cell-free fermentation filtrate and PPM are sequentially and progressively added into a three-stage modified MS culture medium, so that high-efficiency culture of the explant of the plica rugosa in an open environment is realized, the pollution rate and the browning degree are reduced, meanwhile, healing of the explant, adventitious bud differentiation and root primordium formation are promoted, and the seedling rate of open tissue culture of the plica rugosa is increased.

The invention discloses an intelligent identification and calculation method for growth phenotypic characteristics of edible fungi in a budding period, and relates to the technical field of modern facility agriculture. Comprising the following steps: shooting and obtaining a clear RGB image of field growth in the edible mushroom germination period; performing contrast enhancement processing on the clear RGB image; accurate identification is carried out through an improved instance segmentation model RSL-YOLOv8m-Seg to obtain a bud primordium mask image; traversing and segmenting each bud primordium mask one by one, and extracting the contour of the mask; reconstructing a bud primordium contour by adopting a contour fitting method based on each mask contour; extracting morphological characteristics of the bud primordium; and counting growth phenotypic characteristics in a budding period. According to the method, the growth characteristics of the edible fungi in the germination period can be accurately calculated, and a phenotypic characteristic basis is provided for intelligent and accurate management and control of an edible fungi house.

A cultivation method for improving the quality and yield of black fungus using hydrogen-rich water involves spraying a hydrogen-rich water composition three times during mycelial culture, spraying it every two days during primordium differentiation, and spraying it daily during fruiting body enlargement. The hydrogen-rich water composition comprises hydrogen-rich water, xylose, humic acid, gelatin, hydroxypropyl cellulose, and deionized water. This invention effectively solves the problems of poor hydrogen concentration stability and hydrogen volatilization in hydrogen-rich water by adding functional components such as gelatin, hydroxypropyl cellulose, xylose, and humic acid to the hydrogen-rich water, thus extending the time of hydrogen concentration stability and its effect. By using the above-mentioned hydrogen-rich water composition during the mycelial growth and primordium differentiation stages of black fungus, the microenvironment for black fungus growth is comprehensively optimized, resulting in increased yield, improved quality, and enhanced marketability. This provides an effective way to cultivate superior black fungus varieties.

[Problem] To provide a matsutake primordium having a high methyl cinnamate content and a method capable of obtaining such a matsutake primordium having a high methyl cinnamate content at an artificially high yield. [Solution] Cells having the ability to form a primordium that have been isolated from matsutake fruiting bodies are cultured in a medium containing a protein degradation product and having a specific phenylalanine concentration.

The present application relates to the technical field of genetic engineering, and particularly relates to a fruiting inhibition engineering bacterium and a construction method and application thereof, comprising overexpressing a transcription factor Pcc1 in a ganoderma strain, constructing an engineering bacterium MKLGE253241 with high expression of Pcc1, a preservation unit: China General Microbiological Culture Collection Center, address: No. 1, Xibei Road, No. 3, Chaoyang District, Beijing, preservation date: March 11, 2026, preservation number: CGMCC No. 42592. The present application blocks the formation of fruiting body primordium from the molecular level, maintains the mycelium in vegetative growth, and increases the average biomass by 15.2%, without fruiting body; meanwhile, the leather flatness of the mycelium produced by the engineering bacterium is significantly improved, and the scale qualified rate is obviously improved. The present application fundamentally solves the problem of fruiting body leather, avoids chemical inhibitors, and provides a new path for high-performance bio-based leather.

The present application belongs to the technical field of edible mushroom cultivation, and particularly relates to a method for cultivating Armillaria mellea fruiting bodies by using old Gastrodia elata culture material, wherein the cultivation process of Armillaria mellea is optimized, so that the Armillaria mellea primordium and fruiting body induction rate can be provided, a large amount of Armillaria mellea can be cultured, the old Gastrodia elata culture material can be reused, the resource waste can be reduced, the ecological environment can be effectively protected, the economic income of the grower can be improved, the industrial development enthusiasm can be enhanced, the high-quality development and sustainable development of the Gastrodia elata industry can be promoted, and the development and utilization of the Armillaria mellea resource are laid a foundation.

The invention discloses a morchella hypha powder prefabrication method and equipment line, belongs to the technical field of morchella hyphae, and solves the problems that morchella is extremely sensitive to continuous cropping, metabolite changes the soil microenvironment, hypha division and nutrient absorption are directly hindered, the hypha growth speed is reduced by 30%-50%, the biomass is reduced, and primordium formation is delayed. Comprising the following steps: S1, carrying out stress-resistant intensified culture on liquid strains; chitosan in a specific proportion in a liquid strain neutralizes dopamine quinone, antioxidant enzyme is induced to be improved, fat-soluble toxin is embedded in beta-cyclodextrin in a solid culture medium, dendrobium nobile residues adsorb water-soluble toxin, sodium selenite enhances oxidation resistance, germanium dioxide promotes hypha branching, an overburden filtrate degrades dopamine quinone, ash hydrolyzes related toxin, and the yield is increased. Pseudomonas compensates for beneficial metabolites and accelerates metabolism through temperature difference stimulation, so that hypha growth is accelerated, differential metabolites are reduced, biomass is increased, and the three-crop fruiting rate is stable.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.

The invention discloses a hot-start high-specificity LAMP (loop-mediated isothermal amplification) isothermal nucleic acid rapid detection method which comprises the following operation steps: step 1, preparing three pairs of LAMP amplification primers, the 3'ends of one pair of primers F3 and B3 being one of ddC / ddG / ddT / ddA or other closed groups, and each of the primers F3 and B3 internally containing an RNA (ribonucleic acid) basic group; 2, establishing an LAMP isothermal nucleic acid amplification reaction system by using three pairs of primers; 3, in the process of hot start of the high-specificity LAMP amplification reaction, after the primers F3 and B3 are paired with the template DNA, cutting off the DNA at the RNA base by thermal stability endonuclease HII to generate a double-dumbbell initiator (a product 6 in the figure 1), thereby starting the LAMP continuous amplification reaction; if the primers F3 and B3 cannot be specifically paired with the DNA template, the primers F3 and B3 cannot be cut by thermal stability endonuclease HII, and a double-dumbbell type initiator cannot be generated, so that the LAMP continuous amplification reaction cannot be started.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

The invention discloses a method for breeding by culturing sporocarp under pure culture conditions of boletus sinensis, and belongs to the technical field of edible mushroom breeding and cultivation. The method comprises the following steps: activating the boletus sinensis strain JSJ-Bx1, inoculating the boletus sinensis strain JSJ-Bx1 into a culture medium for dark culture, then performing low-temperature induction culture on an activated flat plate which is nearly overgrown with hyphae, changing the hyphae from white to golden yellow and generating hypha kinking, then transferring the hypha flat plate into a fruiting room for culture, and after obvious primordium differentiation is seen, inoculating the hyphae into a culture medium for culture, thereby obtaining the boletus sinensis strain JSJ-Bx1. Continuously performing induced culture to develop into mature sporocarp; sporocarp basidiospores are collected, diluted and coated in a B1 culture medium for germination culture, and monospora hyphae and polyspora hyphae are obtained and serve as stock seeds for breeding the boletus sinensis. According to the method, the mature sporocarp of the boletus sinensis can be directly and rapidly cultured in the agar culture medium, the harvested basidiospore can normally germinate and grow, and the whole life history of the boletus sinensis can be completed under the pure culture condition.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

The invention discloses an intelligent cultivation method for phellinus igniarius based on environmental parameter regulation and control, and particularly relates to the cross technical field of intelligent agriculture and fungus cultivation, and the method comprises the following steps: constructing a phellinus igniarius growth prediction model; respectively setting differentiated target environment parameter range sets for a mycelium development period, a primordium formation period and a sporocarp growth period; in the cultivation process, environmental data are monitored in real time and input into the model to obtain a predicted growth state, and a regulation and control instruction is generated by comparing the predicted growth state with an expected state, so that environmental parameters are maintained in a target range; when the mycelium reaches physiological maturity, a sporocarp differentiation program is automatically triggered, and an environment target is switched; in the later growth period of the sporocarp, morphological characteristics are analyzed through images and compared with standard morphological characteristics, if the deviation exceeds a threshold value, a parameter optimization instruction is generated, and an environment parameter set for optimizing the growth period of the sporocarp is fed back. Precision, automation and intelligence of phellinus igniarius cultivation are achieved, and the yield and quality are improved.

The invention provides a gene for regulating and controlling differentiation of a plant spikelet primordium to seta and application, and belongs to the technical field of molecular biology. The invention provides a protein for regulating and controlling differentiation of a plant spikelet primordium to seta. The protein comprises an amino acid sequence as shown in SEQ ID No. 1. The invention also provides a gene sequence for coding the protein, and a biological material for regulating and controlling the differentiation of the plant spikelet primordium to the seta is prepared on the basis of the gene sequence or the protein, and the transformation of the plant seta to the grain can be regulated and controlled by utilizing the biological material. After the coding gene of the protein is knocked out, differentiation of a part of spikelet primordia to seta can be successfully inhibited, and the part of spikelet primordia which are not differentiated into seta is differentiated into spikelet with stems. The protein and the gene are excavated, so that the molecular mechanism of seta development of the millet can be filled, and meanwhile, the high-yield breeding process of the millet can be promoted.

The invention discloses a fungus expression vector containing a green fluorescent protein reporter gene as well as a construction method and application of the fungus expression vector, and belongs to the technical field of edible fungus genetic engineering. On the basis of flammulina velutipes strain 0747 transcriptome data, a promoter sequence of a gene hyd9 efficiently expressed in hyphae is obtained through screening and cloning, and the promoter sequence has conserved structures such as a TATA box and a CAAT box. The expression activity of the promoter in hyphae is about 60 times that of an Ffgpd promoter. By constructing a hyd9-driven egfp expression vector and converting flammulina velutipes, the result shows that a transformant presents strong green fluorescence, the egfp transcriptional level is significantly higher than that of a Glgpd and Ffgpd promoter control group, a fluorescence signal is weakened in primordium and sporocarp but still can be detected, the mode is consistent with an endogenous hyd9'hypha high expression-sporocarp low expression 'mode, and the expression of the endogenous hyd9-sporocarp low expression is consistent with that of the endogenous hyd9. The promoter is proved to be capable of driving the expression of exogenous genes in a space-time specific manner.

The invention relates to the technical field of agricultural cultivation, in particular to a stropharia rugoso-annulata culture medium prepared from highland barley straw and a preparation method, and the stropharia rugoso-annulata culture medium is prepared from the highland barley straw, modified bentonite, modified beta-cyclodextrin, wheat bran, dry cow dung, calcium superphosphate, monopotassium phosphate and gypsum. The modified bentonite adsorbs phenolic substances, boric acid groups on the surface of the modified beta-cyclodextrin and o-diphenolic hydroxyl groups form covalent bonds to carry out double locking on the phenolic substances, and the cavity of the modified beta-cyclodextrin is wrapped, so that the phenolic substances are prevented from being decomposed at high temperature. Monopotassium phosphate forms a phosphate buffer system at the initial stage of shred production, acidification at the initial stage of hypha metabolism is resisted, and boric acid ester bond hydrolysis is inhibited. Along with the consumption of phosphate radicals by acid production through hypha metabolism, the buffer capacity of monopotassium phosphate loses efficacy, the pH is naturally reduced from the later stage of filamentation to the original base stage, borate bonds are hydrolyzed, hydrophobicity of cetyltrimethylammonium bromide is weakened, and phenolic substances are released through synergism of borate bonds and cetyltrimethylammonium bromide.

The invention relates to an indoor planting method of Xiaoxiang fungi, and belongs to the technical field of edible fungus planting.The indoor planting method includes the following steps that 1, weed tree sawdust is smashed into particles, the processed weed tree sawdust, bran, composite nutrient particles, gypsum powder and lime are mixed, the water content is adjusted to 55%-60%, and a Xiaoxiang fungi planting substrate is obtained; (2) bagging the planting substrate, and sterilizing for later use; (3) after the strain is inoculated, placing the strain in an environment at 22-24 DEG C for dark light culture until hyphae grow all over 2 / 3 of a strain bag, and starting blue light irradiation at night for 3-5 days; (4) after the bags are full of hyphae, management is performed according to color changing conditions until brown mycoderma are uniformly formed on the surfaces of the fungus bags, and then bud pressing treatment is performed; and (5) when the primordium is differentiated and the length of the stipe reaches 0.5-1.0 cm, starting to carry out green light irradiation at night for 8-10 hours every day. The production cycle is remarkably shortened through blue light pre-excitation, the commodity rate is directly improved through green light shape promotion, continuous high yield is guaranteed through nutrient sustained-release particles, and economic benefits are remarkably improved.

The invention relates to the technical field of plant genetic engineering, and particularly discloses application of a GhCOL2 gene in regulation and control of cotton flower primordium differentiation. The coding sequence of the GhCOL2 gene is as shown in SEQ ID No.1 (sequence identifier number 1). According to the invention, the GhCOL2 gene is knocked out by using sgRNA target sequences as shown in SEQ ID No.2 and SEQ ID No.3 through a CRISPR / Cas9 gene editing technology, so that the quantity of the cotton flower primordia can be effectively increased. The invention provides new gene resources and technical means for cotton flower organ development regulation and breeding, and has important agricultural application value.

The invention belongs to the technical field of edible mushroom planting, and particularly relates to a cultivation method for morchella in a cold shed. According to the method, the rice straw is uniformly scattered into the field after being pretreated, and the soil conditioner is applied, so that the content of organic matters in soil can be remarkably increased, the physical structure of the soil can be improved, a soil environment more suitable for growth of morchella can be created, and growth and development of hyphae can be promoted. Bacillus altitudinis and cochlea stickii in the microbial agent can significantly promote the growth speed and biomass accumulation of morchella hyphae, help morchella to better adapt to the environment, and improve the utilization efficiency of the morchella to the substrate. The illumination management mode can effectively stimulate the formation of morchella primordium and the growth of sporocarp, improve the quality of morchella, and make the morchella uniform in color and good in shape.

The invention discloses a domesticated strain of morchella septimelata and application thereof, and relates to the technical field of edible fungi, the strain is morchella septimelata YYZQ-220421, the strain is preserved in the China Center for Type Culture Collection on July 3, 2024, and the preservation number is CCTCC NO: M 20241468. Wild morchella septimelata from Zaoqu county is used as a parent, an excellent strain is obtained through tissue isolation, mating test, fruiting test, product ratio test, regional test and continuous breeding and domestication for many years, the strain is named as YYZQ-220421, the strain has wide environment adaptability and good primordium forming ability, and compared with a reference strain, the strain has the advantages that the yield is high, and the yield is high. The yield is high, the fruiting is early, the fruiting period is long, the commodity is good, the sporocarp is nearly oval, the pileus is medium brown, the ribs are dense, and the stipe is yellowish white, trapezoidal, thin and short.

The present invention relates to a graft material comprising a complex of a plurality of kidney primordia with bladder primordia, the graft material being characterized in that the plurality of kidney primordia with bladder primordia are joined by respective bladder primordia to form a complex.

The invention relates to the technical field of cordyceps militaris culture, in particular to a culture method for planting cordyceps militaris through silkworms, which comprises the following steps: activating a strain through a solid culture medium, transferring into a liquid culture medium, and carrying out shake culture to obtain a hypha suspension; inoculating the bacterial liquid to a silkworm chrysalis substrate subjected to composite coating pretreatment in a sterile environment, and carrying out dark culture under a first condition of low temperature and light shielding until hyphae overgrow; and then converting to a second condition with day and night temperature difference and scattered light to induce primordium formation, and then adjusting to a third condition with constant temperature and high humidity to promote fruiting bodies to be mature and then harvesting. According to the cultivation technology, pollution can be effectively reduced, growth can be promoted, the yield can be effectively increased, the quality of cordyceps militaris sporocarp can be remarkably improved, and the content of main active ingredients, namely cordycepin and polysaccharide, of cordyceps militaris can reach the high level.

The invention belongs to the field of edible mushrooms, and particularly discloses a domesticated wild morchella esculenta strain Jiangnong morchella esculenta strain Y5 and high-yield cultivation of the domesticated wild morchella esculenta strain Y5, and the preservation number of the strain is CGMCC No.41064. The invention further discloses a preparation method of the domesticated wild morchella esculenta strain Y5. The Jiannong morchella esculenta Y5 strain has short-term temperature stress resistance of resisting atmosphere 40 DEG C and soil 28 DEG C in the bud forming and mushroom body growth stage, and compared with existing cultivated species morchella esculenta and morchella sextelata, the Jiannong morchella esculenta Y5 strain has the advantages that a large number of primordium death, black young mushroom tip, yellow stipe, black young mushroom pileus and the like are avoided, and the yield of the Jiannong morchella esculenta Y5 strain is increased. The primordium is early in appearing time, long in harvesting time and high in yield. Meanwhile, the invention further discloses a molecular marker of the Gannong morchella esculenta Y5, and the identification of the specificity and authenticity of the Gannong morchella esculenta Y5 is realized.

The application discloses a high-efficiency genetic transformation method of Agrobacterium rhizogenes mediated by a RUBY reporter gene, and the method uses a bud primordium of a sugarcane seedling as a transformation receptor, uses the Agrobacterium rhizogenes K599 strain carrying a RUBY visualized reporter gene, and obtains positive transgenic materials through direct injection method, co-culture and resistance screening. The application optimizes the infection receptor site, the concentration of the bacterial liquid and the infection mode: the bud primordium is determined as the best infection site; the optimal bacterial liquid concentration is OD600=0.6, and the conversion efficiency is best, reaching 36.67%; on this basis, the infection efficiency can be significantly improved by using 0.02 MPa negative pressure vacuum treatment for 2 minutes, and the positive rate is increased to 55.00%. The betalain produced by the RUBY reporter gene expression realizes the visualized screening of the transformation event, and the method has the advantages of simple operation, short cycle, low cost, high efficiency, visualization and no limitation of the sugarcane genotype, and the like, and provides a high-efficiency and reliable technical platform for the gene function verification and molecular breeding of the sugarcane.

The application discloses a method for cultivating fruit bodies of Chinese saprobic boletus under pure culture conditions, and belongs to the technical field of edible fungus breeding and cultivation. The method comprises the following steps: inoculating a Chinese saprobic boletus strain JSJ-Bx1 after activation into a culture medium for dark culture, then performing low-temperature induction culture on the plate with activated mycelium close to full growth, changing the mycelium from white to golden yellow, and causing mycelium kinking, then transferring the mycelium plate to a fruiting room for culture, continuing to induce culture to develop into mature fruit bodies after obvious primordium differentiation is observed, and collecting fruit body basidiospores, diluting and coating the basidiospores on a B1 culture medium for germination culture, obtaining pseudomonosporic mycelium and polysporic mycelium, and using the pseudomonosporic mycelium and polysporic mycelium as the original strain for Chinese saprobic boletus breeding. The application can directly and rapidly culture Chinese saprobic boletus mature fruit bodies on an agar culture medium, the harvested basidiospores can normally germinate and grow, and the Chinese saprobic boletus can complete the whole life history under pure culture conditions.