45 results about "Primordium" patented technology

The invention discloses an edible fungus multi-parameter growth model construction and environment intelligent optimization method based on deep learning, and the method comprises the steps: collecting environment data, such as temperature, relative humidity, carbon dioxide concentration, illumination intensity, and matrix moisture content, and hypha and sporocarp images; the growth state index is constructed through weighted fusion of hypha respiration intensity, primordium density, pileus color gradient and other characteristics and is used for representing the growth health degree. And predicting a future growth trend based on an image and environment dual-channel feature fusion model, and obtaining prediction credibility according to a prediction error. An environment regulation and control strategy is established according to growth state improvement, energy consumption and physiological risks, equipment control instructions of ventilation, humidification, dehumidification, light supplement and the like are output, and dynamic optimization of a multi-parameter environment is achieved; and meanwhile, the model is updated online in combination with feedback data, so that the system has the capability of adapting to environment change and strain difference. The system can realize accurate monitoring and automatic regulation and control of the growth state of the edible mushrooms, and is suitable for industrialized production of the edible mushrooms.

The invention provides an open type tissue culture method for plica rugosa, which comprises the following steps: S1, performing disinfection treatment on a plica rugosa explant to obtain a pretreated explant; s2, the pretreated explant is inserted into a first modified MS culture medium for first-stage culture, and a first-stage explant is obtained; s3, transferring the first-stage explant into a second modified MS culture medium for second-stage culture to obtain a second-stage explant; s4, transferring the second-stage explant into a third modified MS culture medium for third-stage culture to obtain a primary seedling. According to the method, cell-free fermentation filtrate and PPM are sequentially and progressively added into a three-stage modified MS culture medium, so that high-efficiency culture of the explant of the plica rugosa in an open environment is realized, the pollution rate and the browning degree are reduced, meanwhile, healing of the explant, adventitious bud differentiation and root primordium formation are promoted, and the seedling rate of open tissue culture of the plica rugosa is increased.

A cultivation method for improving the quality and yield of black fungus using hydrogen-rich water involves spraying a hydrogen-rich water composition three times during mycelial culture, spraying it every two days during primordium differentiation, and spraying it daily during fruiting body enlargement. The hydrogen-rich water composition comprises hydrogen-rich water, xylose, humic acid, gelatin, hydroxypropyl cellulose, and deionized water. This invention effectively solves the problems of poor hydrogen concentration stability and hydrogen volatilization in hydrogen-rich water by adding functional components such as gelatin, hydroxypropyl cellulose, xylose, and humic acid to the hydrogen-rich water, thus extending the time of hydrogen concentration stability and its effect. By using the above-mentioned hydrogen-rich water composition during the mycelial growth and primordium differentiation stages of black fungus, the microenvironment for black fungus growth is comprehensively optimized, resulting in increased yield, improved quality, and enhanced marketability. This provides an effective way to cultivate superior black fungus varieties.

[Problem] To provide a matsutake primordium having a high methyl cinnamate content and a method capable of obtaining such a matsutake primordium having a high methyl cinnamate content at an artificially high yield. [Solution] Cells having the ability to form a primordium that have been isolated from matsutake fruiting bodies are cultured in a medium containing a protein degradation product and having a specific phenylalanine concentration.

The present application relates to the technical field of genetic engineering, and particularly relates to a fruiting inhibition engineering bacterium and a construction method and application thereof, comprising overexpressing a transcription factor Pcc1 in a ganoderma strain, constructing an engineering bacterium MKLGE253241 with high expression of Pcc1, a preservation unit: China General Microbiological Culture Collection Center, address: No. 1, Xibei Road, No. 3, Chaoyang District, Beijing, preservation date: March 11, 2026, preservation number: CGMCC No. 42592. The present application blocks the formation of fruiting body primordium from the molecular level, maintains the mycelium in vegetative growth, and increases the average biomass by 15.2%, without fruiting body; meanwhile, the leather flatness of the mycelium produced by the engineering bacterium is significantly improved, and the scale qualified rate is obviously improved. The present application fundamentally solves the problem of fruiting body leather, avoids chemical inhibitors, and provides a new path for high-performance bio-based leather.

The present application belongs to the technical field of edible mushroom cultivation, and particularly relates to a method for cultivating Armillaria mellea fruiting bodies by using old Gastrodia elata culture material, wherein the cultivation process of Armillaria mellea is optimized, so that the Armillaria mellea primordium and fruiting body induction rate can be provided, a large amount of Armillaria mellea can be cultured, the old Gastrodia elata culture material can be reused, the resource waste can be reduced, the ecological environment can be effectively protected, the economic income of the grower can be improved, the industrial development enthusiasm can be enhanced, the high-quality development and sustainable development of the Gastrodia elata industry can be promoted, and the development and utilization of the Armillaria mellea resource are laid a foundation.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.

The invention discloses a hot-start high-specificity LAMP (loop-mediated isothermal amplification) isothermal nucleic acid rapid detection method which comprises the following operation steps: step 1, preparing three pairs of LAMP amplification primers, the 3'ends of one pair of primers F3 and B3 being one of ddC / ddG / ddT / ddA or other closed groups, and each of the primers F3 and B3 internally containing an RNA (ribonucleic acid) basic group; 2, establishing an LAMP isothermal nucleic acid amplification reaction system by using three pairs of primers; 3, in the process of hot start of the high-specificity LAMP amplification reaction, after the primers F3 and B3 are paired with the template DNA, cutting off the DNA at the RNA base by thermal stability endonuclease HII to generate a double-dumbbell initiator (a product 6 in the figure 1), thereby starting the LAMP continuous amplification reaction; if the primers F3 and B3 cannot be specifically paired with the DNA template, the primers F3 and B3 cannot be cut by thermal stability endonuclease HII, and a double-dumbbell type initiator cannot be generated, so that the LAMP continuous amplification reaction cannot be started.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

The invention discloses a method for breeding by culturing sporocarp under pure culture conditions of boletus sinensis, and belongs to the technical field of edible mushroom breeding and cultivation. The method comprises the following steps: activating the boletus sinensis strain JSJ-Bx1, inoculating the boletus sinensis strain JSJ-Bx1 into a culture medium for dark culture, then performing low-temperature induction culture on an activated flat plate which is nearly overgrown with hyphae, changing the hyphae from white to golden yellow and generating hypha kinking, then transferring the hypha flat plate into a fruiting room for culture, and after obvious primordium differentiation is seen, inoculating the hyphae into a culture medium for culture, thereby obtaining the boletus sinensis strain JSJ-Bx1. Continuously performing induced culture to develop into mature sporocarp; sporocarp basidiospores are collected, diluted and coated in a B1 culture medium for germination culture, and monospora hyphae and polyspora hyphae are obtained and serve as stock seeds for breeding the boletus sinensis. According to the method, the mature sporocarp of the boletus sinensis can be directly and rapidly cultured in the agar culture medium, the harvested basidiospore can normally germinate and grow, and the whole life history of the boletus sinensis can be completed under the pure culture condition.

The invention discloses an intelligent cultivation method for phellinus igniarius based on environmental parameter regulation and control, and particularly relates to the cross technical field of intelligent agriculture and fungus cultivation, and the method comprises the following steps: constructing a phellinus igniarius growth prediction model; respectively setting differentiated target environment parameter range sets for a mycelium development period, a primordium formation period and a sporocarp growth period; in the cultivation process, environmental data are monitored in real time and input into the model to obtain a predicted growth state, and a regulation and control instruction is generated by comparing the predicted growth state with an expected state, so that environmental parameters are maintained in a target range; when the mycelium reaches physiological maturity, a sporocarp differentiation program is automatically triggered, and an environment target is switched; in the later growth period of the sporocarp, morphological characteristics are analyzed through images and compared with standard morphological characteristics, if the deviation exceeds a threshold value, a parameter optimization instruction is generated, and an environment parameter set for optimizing the growth period of the sporocarp is fed back. Precision, automation and intelligence of phellinus igniarius cultivation are achieved, and the yield and quality are improved.

The invention discloses a fungus expression vector containing a green fluorescent protein reporter gene as well as a construction method and application of the fungus expression vector, and belongs to the technical field of edible fungus genetic engineering. On the basis of flammulina velutipes strain 0747 transcriptome data, a promoter sequence of a gene hyd9 efficiently expressed in hyphae is obtained through screening and cloning, and the promoter sequence has conserved structures such as a TATA box and a CAAT box. The expression activity of the promoter in hyphae is about 60 times that of an Ffgpd promoter. By constructing a hyd9-driven egfp expression vector and converting flammulina velutipes, the result shows that a transformant presents strong green fluorescence, the egfp transcriptional level is significantly higher than that of a Glgpd and Ffgpd promoter control group, a fluorescence signal is weakened in primordium and sporocarp but still can be detected, the mode is consistent with an endogenous hyd9'hypha high expression-sporocarp low expression 'mode, and the expression of the endogenous hyd9-sporocarp low expression is consistent with that of the endogenous hyd9. The promoter is proved to be capable of driving the expression of exogenous genes in a space-time specific manner.

The invention relates to an indoor planting method of Xiaoxiang fungi, and belongs to the technical field of edible fungus planting.The indoor planting method includes the following steps that 1, weed tree sawdust is smashed into particles, the processed weed tree sawdust, bran, composite nutrient particles, gypsum powder and lime are mixed, the water content is adjusted to 55%-60%, and a Xiaoxiang fungi planting substrate is obtained; (2) bagging the planting substrate, and sterilizing for later use; (3) after the strain is inoculated, placing the strain in an environment at 22-24 DEG C for dark light culture until hyphae grow all over 2 / 3 of a strain bag, and starting blue light irradiation at night for 3-5 days; (4) after the bags are full of hyphae, management is performed according to color changing conditions until brown mycoderma are uniformly formed on the surfaces of the fungus bags, and then bud pressing treatment is performed; and (5) when the primordium is differentiated and the length of the stipe reaches 0.5-1.0 cm, starting to carry out green light irradiation at night for 8-10 hours every day. The production cycle is remarkably shortened through blue light pre-excitation, the commodity rate is directly improved through green light shape promotion, continuous high yield is guaranteed through nutrient sustained-release particles, and economic benefits are remarkably improved.

The invention relates to the technical field of plant genetic engineering, and particularly discloses application of a GhCOL2 gene in regulation and control of cotton flower primordium differentiation. The coding sequence of the GhCOL2 gene is as shown in SEQ ID No.1 (sequence identifier number 1). According to the invention, the GhCOL2 gene is knocked out by using sgRNA target sequences as shown in SEQ ID No.2 and SEQ ID No.3 through a CRISPR / Cas9 gene editing technology, so that the quantity of the cotton flower primordia can be effectively increased. The invention provides new gene resources and technical means for cotton flower organ development regulation and breeding, and has important agricultural application value.

The invention belongs to the technical field of edible mushroom planting, and particularly relates to a cultivation method for morchella in a cold shed. According to the method, the rice straw is uniformly scattered into the field after being pretreated, and the soil conditioner is applied, so that the content of organic matters in soil can be remarkably increased, the physical structure of the soil can be improved, a soil environment more suitable for growth of morchella can be created, and growth and development of hyphae can be promoted. Bacillus altitudinis and cochlea stickii in the microbial agent can significantly promote the growth speed and biomass accumulation of morchella hyphae, help morchella to better adapt to the environment, and improve the utilization efficiency of the morchella to the substrate. The illumination management mode can effectively stimulate the formation of morchella primordium and the growth of sporocarp, improve the quality of morchella, and make the morchella uniform in color and good in shape.

The invention relates to the technical field of cordyceps militaris culture, in particular to a culture method for planting cordyceps militaris through silkworms, which comprises the following steps: activating a strain through a solid culture medium, transferring into a liquid culture medium, and carrying out shake culture to obtain a hypha suspension; inoculating the bacterial liquid to a silkworm chrysalis substrate subjected to composite coating pretreatment in a sterile environment, and carrying out dark culture under a first condition of low temperature and light shielding until hyphae overgrow; and then converting to a second condition with day and night temperature difference and scattered light to induce primordium formation, and then adjusting to a third condition with constant temperature and high humidity to promote fruiting bodies to be mature and then harvesting. According to the cultivation technology, pollution can be effectively reduced, growth can be promoted, the yield can be effectively increased, the quality of cordyceps militaris sporocarp can be remarkably improved, and the content of main active ingredients, namely cordycepin and polysaccharide, of cordyceps militaris can reach the high level.

The application discloses a high-efficiency genetic transformation method of Agrobacterium rhizogenes mediated by a RUBY reporter gene, and the method uses a bud primordium of a sugarcane seedling as a transformation receptor, uses the Agrobacterium rhizogenes K599 strain carrying a RUBY visualized reporter gene, and obtains positive transgenic materials through direct injection method, co-culture and resistance screening. The application optimizes the infection receptor site, the concentration of the bacterial liquid and the infection mode: the bud primordium is determined as the best infection site; the optimal bacterial liquid concentration is OD600=0.6, and the conversion efficiency is best, reaching 36.67%; on this basis, the infection efficiency can be significantly improved by using 0.02 MPa negative pressure vacuum treatment for 2 minutes, and the positive rate is increased to 55.00%. The betalain produced by the RUBY reporter gene expression realizes the visualized screening of the transformation event, and the method has the advantages of simple operation, short cycle, low cost, high efficiency, visualization and no limitation of the sugarcane genotype, and the like, and provides a high-efficiency and reliable technical platform for the gene function verification and molecular breeding of the sugarcane.

The application discloses a method for cultivating fruit bodies of Chinese saprobic boletus under pure culture conditions, and belongs to the technical field of edible fungus breeding and cultivation. The method comprises the following steps: inoculating a Chinese saprobic boletus strain JSJ-Bx1 after activation into a culture medium for dark culture, then performing low-temperature induction culture on the plate with activated mycelium close to full growth, changing the mycelium from white to golden yellow, and causing mycelium kinking, then transferring the mycelium plate to a fruiting room for culture, continuing to induce culture to develop into mature fruit bodies after obvious primordium differentiation is observed, and collecting fruit body basidiospores, diluting and coating the basidiospores on a B1 culture medium for germination culture, obtaining pseudomonosporic mycelium and polysporic mycelium, and using the pseudomonosporic mycelium and polysporic mycelium as the original strain for Chinese saprobic boletus breeding. The application can directly and rapidly culture Chinese saprobic boletus mature fruit bodies on an agar culture medium, the harvested basidiospores can normally germinate and grow, and the Chinese saprobic boletus can complete the whole life history under pure culture conditions.

The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods.A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.

This invention discloses a SNP marker and detection primer pair associated with the adventitious root primordium trait of *Senecio scandens* with stem nodules, and its application, belonging to the field of molecular biology technology. Based on SNP marker technology, this invention develops an SNP marker and detection primer pair linked to the adventitious root primordium trait of *Senecio scandens* with stem nodules. This invention involves phenotypic identification of mutants induced by EMS mutagenesis in *Senecio scandens* with stem nodules, followed by isolation, purification, population construction, and genetic analysis combined with Mutmap. + The SNP marker associated with adventitious root primordia is located at 10,433,968 bp on chromosome 4 of *Senecio scandens*, involving a C / T mutation. When the genotype is T, *Senecio scandens* lacks adventitious root primordia. This invention's SNP molecular marker can screen F2 populations at the early cotyledon stage, identifying mutants and wild types without phenotypic differences, thus improving screening efficiency, shortening the breeding cycle, and demonstrating value in marker-assisted breeding.

Epithelial stem cells (15), such as hair follicle epithelial stem cells, are cultured on a scaffold (13) comprising a molecule containing an extracellular cadherin (cadherin EC) domain. The epithelial stem cells 15 are then separated from the scaffold 13. The epithelial stem cells 15 are prepared through the abovementioned process. Thereafter, a hair follicle primordium10 may be produced by three-dimensionally culturing the epithelial stem cells 15 and mesenchymal cells 16 in a state in which a layer of the epithelial stem cells 15 and a layer of the mesenchymal cells 16 overlap. The mesenchymal cell 16 layer may include a layer of hair papilla cells and a layer of cells constituted of dermal pluripotent cells (DDMCs) or dermal sheath cells (DS cells). The epithelial stem cell 15 layer may overlap the hair papilla cell-layer side of the mesenchymal cell 16 layer.

A promoter ZmMIR529-pro specifically expressed during the early development of a maize tassel primordium and a use thereof. The nucleotide sequence of the promoter ZmMIR529-pro is as shown in SEQ ID NO: 1, or a nucleotide sequence complementary to the sequence as shown in SEQ ID NO: 1, or a nucleotide sequence having 75% or more homology with the sequence as shown in SEQ ID NO: 1 or the complementary sequence of the sequence as shown in SEQ ID NO: 1. The promoter ZmMIR529-pro specifically expressed during the early development of a maize tassel primordium can initiate the specific expression of a target gene in a maize tassel, thereby avoiding the adverse effect caused by continuous expression of the target gene in plant tissue. The promoter has significant value for specific modification of the traits of maize tassel using a transgenic means and for application in maize genetic breeding.

The application discloses a kind of in vitro culture accelerant of kepler ironland stem tip and application thereof.The accelerant contains trans-zeatin riboside, brassinolide and naphthalene acetic acid with mass ratio of 2.0-3.0:1.0-1.5:0.1-0.5, which can effectively improve the in vitro culture effect of kepler ironland stem tip.The application applies the accelerant to the in vitro rapid propagation of kepler ironland stem tip, and the specific method is as follows: cutting the stem tip of kepler ironland plant for disinfection culture; after exposing leaf primordium and apical growth point of explant, inoculating in adventitious bud culture medium to induce culture to obtain adventitious bud; inoculating the adventitious bud in proliferation culture medium to culture to produce multiple shoots; taking out the multiple shoots and placing in strong seedling culture medium for strong seedling culture to complete propagation; the adventitious bud culture medium, proliferation culture medium and strong seedling culture medium contain the accelerant.The in vitro rapid propagation technology of kepler ironland stem tip based on the accelerant effectively improves the propagation success rate and efficiency of kepler ironland.

The present application relates to edible mushroom cultivation technology field, specifically discloses a kind of cultivation method of gold ear.The present application is by controlling culture temperature, humidity, carbon dioxide concentration, carries out red blue mixed light irradiation in different period, and cooperate with the light intensity of red blue mixed light, and additional spray exogenous nutrient solution in the dark culture stage of color change period and other measures, effectively promote the formation of gold ear fruit body primordium and the whole diameter of gold ear fruit body becomes large, and fully stimulate the color change reaction of mycelium in color change period, so that gold ear fruit body character is good, growth cycle is short, fundamentally solve the yield and quality instability problem existing in the prior art gold ear cultivation technology, meet the needs of modern agricultural fine management and high efficiency output, suitable for gold ear factory cultivation and popularization, it has important significance for promoting the upgrading and development of gold ear cultivation industry.

This invention discloses a segmented cutting propagation method, belonging to the field of garden plant propagation technology. The segmented cutting propagation method includes the following steps: cutting preparation, root primordia-promoting treatment, and root-promoting treatment. The root primordia-promoting treatment involves quickly dipping the lower part of the cutting into a root primordia-promoting solution, placing the cutting on a partition of a root primordia-promoting device, and spraying the cutting on the partition for 9-11 minutes every 2 hours until root primordia are generated, thus obtaining cuttings treated with root primordia-promoting solution. The root primordia-promoting solution is an aqueous solution of IBA, NAA, and 70% hymexazol wettable powder. Applying this segmented cutting propagation method to summer softwood cutting propagation can shorten rooting time and reduce rot rate.

The invention provides a plant frost-preventing and anti-freezing nutritional repairing fertilizer and a preparation method and application thereof, and the raw materials for preparing the plant frost-preventing and anti-freezing nutritional repairing fertilizer comprise, by weight, 35-50 parts of inorganic salt, 5-10 parts of amino acid, 10-20 parts of an active anti-freezing component, 5-15 parts of a plant extract and 10-20 parts of an auxiliary material. The plant frost-preventing and anti-freezing nutritional repairing fertilizer can promote flower primordium splitting, improve the pollen germination rate, reduce flower and fruit falling, lower the freezing point of plant juice, relieve freezing injury and improve the self-recovery capacity of plants after freezing, is suitable for fruit trees, tomatoes and other commercial crops, and can remarkably improve the stress resistance and yield of the plants.

The present application relates to a kind of toxin type plant rooting liquid, rooting method and application, wherein the composition of rooting liquid is poplar canker bacteria toxin, formula is 5.00% poplar canker bacteria toxin aqueous solution, using method is the base of the stem section to be rooted into the rooting liquid, when the stem section base appears root primordium, transplant to soil, rooting rate can reach 83.33%, and more fibrous root, plant growth is robust, seedling survival rate can reach 83.33%, simultaneously, the rooting liquid has the ability of enhancing seedling disease resistance simultaneously.This application not only can efficiently promote plant rooting, improve transplanting survival rate, but also can improve the immunity of plant, low cost, simple to make, convenient to use, remarkable effect.

The present invention discloses a plateau potato tissue culture medium formula, and relates to the technical field of plant tissue culture, the plateau potato tissue culture medium formula comprises the following components and concentration ranges: a basic inorganic salt, the basic inorganic salt comprises a macroelement, a microelement and an iron salt, wherein the macroelements are prepared from the following components: 1800 to 2000 mg / L of potassium nitrate, 1500 to 1800 mg / L of ammonium nitrate, 150 to 200 mg / L of monopotassium phosphate, 350 to 400 mg / L of magnesium sulfate and 400 to 480 mg / L of calcium chloride; according to the plateau potato tissue culture medium formula, an MS culture medium is used as a nutritional basis, and three plant growth regulators, namely indolebutyric acid (IBA), naphthylacetic acid (NAA) and indoleacetic acid (IAA), are subjected to composite compatibility at specific concentrations, so that an optimized plant growth regulator composition is constructed; the synergistic effect of the three auxin in promoting root primordium induction, root thickening and root elongation is utilized, so that the common obstacles of slow rooting, thin and weak root systems and the like of the plateau potato virus-free test-tube plantlets are effectively overcome, and the robust development and the overall growth vigor of the root systems of the plateau potato virus-free test-tube plantlets are promoted.

The present application belongs to the technical field of tea tree cultivation, and discloses a cultivation method for promoting multi-root resistance of asexual tea seedlings, comprising: seedbed preparation; selection and treatment of bud sticks; cutting and water and fertilizer management; composition application: 15-20 days after cutting of the tea seedlings, Trichoderma harzianum is applied by root irrigation, and the fungus is applied periodically twice; 45-50 days after cutting of the tea seedlings, water-soluble fertilizer containing Ascophyllum nodosum is applied through a drip irrigation system, and the fertilizer is applied periodically 2-3 times; seedling hardening. The Trichoderma harzianum is applied at the initial stage of root formation of the tea seedlings to promote root colonization and root primordium differentiation, and the water-soluble fertilizer containing Ascophyllum nodosum is applied after the root formation to provide nutrition, and the two are combined in time to significantly promote multi-rooting of the tea seedlings, enhance the resistance, and improve the survival rate, and the operation is simple, and the method is suitable for large-scale breeding of asexual tea seedlings, and is especially suitable for arid and semi-arid areas in the north.