456 results about "Primordium" patented technology

The method of growing a fungal fruiting body requires exposing a mycelium of a desired organism type to environmental conditions sufficient to induce fruiting of fungal primordium in the organism type followed by enclosing the fungal primordium within a mold of a designated shape representing a near net shape volume of a desired final product. The fungal primordium is allowed to grow and fill the mold to form a mass of fungal tissue equivalent in shape to the designated shape of the mold after which the mass of fungal tissue is removed from the mold and dried.

The method of growing a fungal fruiting body requires exposing a mycelium of a desired organism type to environmental conditions sufficient to induce fruiting of fungal primordium in the organism type followed by enclosing the fungal primordium within a mold of a designated shape representing a near net shape volume of a desired final product. The fungal primordium is allowed to grow and fill the mold to form a mass of fungal tissue equivalent in shape to the designated shape of the mold after which the mass of fungal tissue is removed from the mold and dried.

The invention relates to a pholiota nameko culture method, which comprises the steps of: culture material proportioning and preparation, sterilization, inoculation and bagging, mycelium culture, summer-crossing management and primordium inducement fruiting. The pholiota nameko culture materials comprise the following recipe and mixture ratios: 30 to 50 parts of corncobs, 30 to 50 parts of soft mixed hardwood sawdust, 10 to 20 parts of bran, 2 to 4 parts of corn starch, 0.5 to 1.5 parts of gypsum and 0.5 to 1.5 parts of lime. The culture raw materials adopt the corncobs and the soft mixed hardwood sawdust as major materials, a good basis is laid for the smooth implementation of the Natural Forest Protection Projects and the pholiota nameko planting in flat areas, planting trays are omitted, the culture cost can be saved by 15 percent, the culture process is simple and is easy to implement, the work efficiency is improved, the edge fruiting effect of the pholiota nameko bag type culture is improved by 30 percent through being compared with that of the disc type culture, the edge fruiting effect of the edible fungi is sufficiently utilized, the yield can be improved by more than 10 percent, and the high-quality fruiting rate is improved by more than 40 percent.

The invention discloses a bagged mushroom production method which comprises the steps of strain making, nutrient preparation, bagging, sterilization, inoculation, mycelium culture, coloring, inducement to primordium of mushroom and harvesting. The method is characterized in that 1, in the nutrient preparation step, 78% of miscellaneous tree sawdust, 20% of wheat bran, 1% of sucrose and 1% of gypsum in percentage by weight are evenly mixed, then 55-58% of water is added, and blending is performed; 2, the nutrient is put in barrel bags within 4 hours; 3, the bags are piled in the shape of a well and are sterilized; 4, when the bags are cooled to 25-28 DEG C, inoculation is performed; 5, the mushroom bags are transferred into a mushroom growth room of 20-25 DEG C and subjected to mycelium culture, and puncturing ventilation is performed twice in the mycelium culture period; and 6, in the mushroom growth management period, artificially-aided inducement to primordium of mushroom, dry stimulation and other treatment operations are performed. According to the invention, large-scale bagged mushroom production can be realized, the mushroom survival rate is high, the deformed mushroom defective rate is below 5%, the cost can be reduced and the product quality is high.

The invention provides a preparation method of virus-free seedlings of sweet potato. The method comprises the following steps: placing sweet potato blocks that are exposed under the sun and matured banana together for 3 to 5 days; planting the sweet potatoes in sandy soil to accelerate the germination in a culture box; setting relatively high temperature; picking 1-2cm top end of the seedling based on the length; cutting off the visual leaves; disinfecting the surface; rinsing with sterile water; picking off stem tip with 1 to 2 leaf primordium; inoculating to the corresponding culture medium to respectively induce differentiation of adventitious buds and generation of somatic embryogenesis; performing virus detection to a regeneration plant; performing subculture for the virus-free seedling; hardening-seedling; directly planting into a plastic greenhouse; performing flood irrigation with more water; preventing direct exposing under sun at noon in the first two weeks after transplanting; applying urea three weeks after transplanting; watering to obtain virus-free seedlings. According to the preparation method of the virus-free seedlings of sweet potato, the virus-free test-tube plantlets are acclimated and then directly planted into the plastic greenhouse, thus the survival rate is obviously raised, and the operation processes are decreased; the process of acclimating in an acclimating room is saved, and as a result, labor, materials and property are saved.

The invention discloses a large-scale artificial cultivation method for phellinus igniarius and mainly solves the problem that an existing method can not cultivate sporocarps rapidly in a large-scale mode. The method comprises the following steps: 1, obtaining protospecies, and inoculating the protospecies into a cultivating bag of a phellinus igniarius large-scale artificial medium for cultivation; 2, when phellinus igniarius hyphae overgrow the cultivating bag and the hyphae are deep yellow, or protruding nodular phellinus igniarius primordium starts to appear in the cultivating bag, transferring the cultivating bag full of the hyphae into a fruiting house for conducting fruiting cultivation. The cultivation environment conditions inside the fruiting house is that the relative air humidity ranges from 85% to 98%, and the temperature ranges from 22 DEG C to 28 DEG C. The method has the advantages that the phellinus igniarius sporocarps can be cultivated rapidly in a large-scale mode.

The invention discloses a method for shortening the hypha growth cycle of a pleurotus eryngii fruiting bag and improving yield, which belongs to the technical field of edible mushroom culture. The method comprises the following steps of: the preparation and bagging of a cultivated species culture medium, the preparation and bagging of fruiting bag compost, sterilization, the inoculation and culture of the cultivated species culture medium, the inoculation and culture of the fruiting bag compost, inducement to primordium, mushroom culture, harvesting and the like. Compared with the conventional methods, the method shortens the spawn runing cycle by 20 to 30 days, improves the spawn runing success rate, increases the ratio of high-quality mushrooms as nutrients are completely decomposed and accumulated, improves the biological efficiency to over 70 percent from 35 to 50 percent, shortens the culture cycle to 48 days from about 88 days and remarkably improves economic benefits in the cultivation of pleurotus eryngii, and can be popularized and applied in edible mushroom culture areas.

The invention discloses a pleurotus eryngii factory bottle cultivation method, which comprises the following steps of: inoculating; cultivating bacteria; stimulating mycelium; positioning and inducing primordium; performing bud thinning; breeding, and the like, wherein steps of increasing the mycelium, recovering cultivation, positioning and moistening the surface of the mycelium and rapidly lodging the mycelium are increased after mycelium stimulation and before inducement to the primordium. The cultivation method has a good effect of inducement to the primordium, the problems of increased labor cost, low yield and low degree of automation in the bottle cultivation production process due to redundant emergent buds in the pleurotus eryngii primordium inducement process can be completely solved, and the method is simple in facilities, high in factory level and high in operability and has large-scale popularization prospects.

The invention provides a cultivating method of a poria cocos bag material and relates to a method for cultivating poria cocos without using pine-wood. The method comprises the following steps: grinding the plant straws or branches, mixing evenly with pine wood chips, rice bran, sucrose, calcined gypsum and the like, and manufacturing into a bacteria bag; inoculating the poria cocos strain after the bacteria bag is sterilized and then is placed in a greenhouse to cultivate poria cocos hypha; embedding into a cultivation cellar when the poria cocos bacteria hypha grows and the bacteria bag is filled with the poria cocos bacteria hypha; selecting the intensive part of the hypha body in the bacteria bag to inoculate a small block of tender fresh poria cocos sclerotium block; then earthing and encapsulating the cellar; leading the poria cocos hypha in the fresh sclerotium block to recover the vitality, and fusing with the hypha body in the cultivating bacteria bag to form sclerotium primordium, then growing to form newly born sclerotium. The invention utilizes plant straws or branches rich in cellulose and hemicellulose, pine wood chips, rice bran, sucrose and the like to manufacture into the cultivation bacteria bag to substitute pine wood, thus not only turning waste into wealth, effectively protecting pinery resources, but also needing only 3 to 4 months for the picking time, thereby being beneficial for the circulating and comprehensive utilization of the cultivation site and enlarging the production of poria cocos.

The invention relates to a method for improving yield of straw mushroom, including: (1) raw materials are soaked; (2) the raw materials after being soaked are taken out and piled up, film is used for covering, heat preservation and moisturizing are carried out, and natural piling is carried out for fermentation; (3) the fermented culture material is moved onto a bedstead and covered by the film; (4) culture material is subject to secondary fermentation, and ignited honeycomb briquette is rapidly moved into indoor space, so as to carry out pasteurization; (5) microorganisms are uniformly scattered onto the upper surface of the culture material under open environment; (6) the temperature of material is controlled to be 35-38 DEG C, and air temperature is controlled to be 30-32 DEG C; (7) 'fruiting water' is sprayed five days after planting, and sodium acetate solution is sprayed while 'fruiting water' is sprayed; (8) harvesting is carried out. The invention has the advantages that method is simple, cost is low, formation of straw mushroom primordium can be promoted, the quantity of straw mushroom primodium is increased, yield of unit area is improved by about 17.9% at least, contamination rate is reduced, and biological efficiency is high, thus being beneficial to practical popularization and application.

The invention provides a formula for a pholiota nameko cultivation material. The cultivation material is characterized in that tea seed hull and tea fruit shuck are used as main raw materials, bagasse, rice bran, corn flour, gypsum powder and the like are used as auxiliary materials, and water content in the cultivation material is 63 to 65%. The invention also provides a manufacturing method for the pholiota nameko cultivation material. Compared with a sawdust cultivation material, the pholiota nameko cultivation material provided by the invention has the following advantages: 1, coarse and fine raw materials of the cultivation material are reasonably collocated, so air permeability and water holding capacity of the cultivation material are improved, the mycelial growth speed of pholiota nameko is accelerated, and time for obtaining a pocketful of pholiota nameko is shortened; 2, through natural piling fermentation, substances like lignin, cellulose, semi-cellulose and pectin in the tea seed hull and the tea fruit shuck are decomposed into simple substances which can be easily absorbed by pholiota nameko, so formation of the sporocarp primordium of pholiota nameko is promoted, the quantity of effective pholiota nameko is increased, and the output of pholiota nameko is increased by 25 to 35%; and 3, the sources of the cultivation raw materials are broadened, production cost is reduced, and the formula and the manufacturing method are suitable for being popularized and applied in an oil tea producing area.

The invention relates to an ornamental ganoderma lucidum cultivation method. The ornamental ganoderma lucidum cultivation method is characterized by including the technological processes of tree selection, tree felling, segment cutting, bag making, sterilization, inoculation, spawn running, primordium inducement, ganoderma lucidum cultivation and modeling, pure broad-leaved tree basswood serves as the material, an ornamental ganoderma lucidum variety is selected, ganoderma lucidum liquid spawns are made, and inoculation is performed with a liquid spawn inoculation method. At the ganoderma lucidum spawn running phase and the ganoderma lucidum sporocarp growing and developing phase, the temperature, the humidity, the illumination and the concentration of carbon dioxide of the environment are comprehensively regulated; at the ganoderma lucidum primordium differentiation phase, the ganoderma lucidum stem elongation phase and the ganoderma lucidum pileus differentiation phase, according to the biological characteristics, including phototaxis, regeneration and sensitivity to carbon dioxide, of ganoderma lucidum, the living ganoderma lucidum is artistically modeled respectively through the key technologies of ganoderma lucidum living body grafting, light source induction and ganoderma lucidum pileus regeneration, and then living ganoderma lucidum representing auspicious omen and having high ornamental value is cultivated. The cultivated ganoderma lucidum is natural and ecological, shows the wonderful workmanship excelling nature and manifests unique art forms and cultural connotation of the ganoderma lucidum, the cultivated ganoderma lucidum not only can serve as an upmarket present for relatives and friends but also can be added to a household art collection, the cultivated ganoderma lucidum has wide application prospects and will create good economic benefits and social benefits.

The invention discloses a hericium erinaceus cultivation method comprising the steps of culture material preparation, fungi bag preparation, sterilization, inoculation, greenhouse construction, mycelium culturing, fruiting management, harvesting, and post management. The cultivation process provided by the invention is simple, and the cost is low. The cultivated hericium erinaceus has the advantages of fast mycelium germination, robust growth, uniform and neat primordium, ordered fruiting body growth condition, robust fruiting body growth, and high biological efficiency.

The invention provides an industrialized cultivation method of Morchella importuna. The method is characterized by comprising steps as follows: (1) preparation of a culture substrate; (2) preparation of a liquid strain; (3) mycelium culture; (4) fruiting management: mycelia in the step (3) are transferred to a fruiting room for fruiting management, and the fruiting management comprises management in a primordium formation promoting period, management in a mushroom bud formation period, management in a growth period and management in the manure period. According to the method, crop waste is taken as a raw material for preparation of a culture medium, liquid strains are used for sowing, industrialized cultivation of Morchella is completely realized indoor, the method is simple to operate, low in cost, short in cycle, controllable in quality and capable of realizing industrialized production all year round, and one pollution-free, pleasantly scented and delicious green product is obtained.

The invention provides a cultivation method of hypsizygus marmoreus. The cultivation method comprises the steps of filling, sterilizing, cooling, inoculating, culturing, mycelium stimulation, inducement to primordium, bearing and harvesting, wherein in the step of filling, raw materials are filled in cultivation bottles in a cultivation basket; 25 cultivation bottles are filled in the cultivation basket; the cultivation bottles are distributed in a layout of 5x5; and the volume of each cultivation bottle is 500-700ml. The cultivation method has the following beneficial effects: by reducing the volume of the cultivation bottles and increasing the quantity of cultivation bottles filled in the same cultivation basket, under the condition of ensuring that the hypsizygus marmoreus yield of one cultivation basket is basically equal, the cultivation cycle is substantially shortened, and vast invested construction area and corresponding capitals and goods are saved, thus reducing the cultivation cost of hypsizygus marmoreus; and on the premise that the size of the basket body is basically equal, the space utilization rate in the culture stage of hypsizygus marmoreus can be increased by using the cultivation method provided by the invention instead of the existing cultivation method.

The invention discloses selenium-enriched pleurotus eryngii cultivation and ingredient detecting methods. The cultivation method comprises steps of culture medium preparation, bagging, sterilization, inoculation, fermentation, inducement to primordium, fruiting, harvesting and the like. Through control of main cultivation conditions of temperature, humidity, illumination, ventilation and the like, pleurotus eryngii is rich in selenium, can be produced all year round and comes into the market all year round, so that the yield is improved, the economic benefit is increased, the selenium enrichment capacity of pleurotus eryngii is improved, and the dietary therapy value of pleurotus eryngii is improved. The ingredient detecting method comprises detection of selenium elements, amino acid and total sugar, the amount of selenium of each kilogram of selenium-enriched pleurotus eryngii is 600 mu g / g, the selenium enrichment capacity is the best, and the amount of selenium doesn't exceed the limit absorption range, which is 800 mu g / d and is also the poisoning limit, of a human body.

The invention discloses a bottle cultivation method for Hypsizigus marmoreus. The bottle cultivation method for the Hypsizigus marmoreus comprises the following steps of: inoculating the Hypsizigus marmoreus on a bottled culture medium, wherein the inoculation ratio is that each bottle of fungal strains are inoculated on 32 to 40 bottles of culture bottles; performing mycelium culture under the conditions of temperature of between 18 and 25 DEG C, humidity of 60 to 80 percent and CO2 concentration of 1,500 to 5,500 ppm; after the Hypsizigus marmoreus are matured, performing mycelium stimulation and supplementing a small amount of water; performing inducement to primordium; after mushroom buds are generated, performing inhibition period culture for 3 to 5 days; when the mushroom buds grow out of the bottleneck, sleeving a plastic sleeve; performing elongating stage culture; and harvesting after the harvesting standard is reached. In the bottle cultivation method, the period is 105 to 125 days and average yield of a single bottle is 300 to 450g. The bottle cultivation method is a factory-like bottle cultivation method using sawdust, cotton seed hulls and corn cobs as main raw materials. By the bottle cultivation method, the mechanization level and the automation degree of the Hypsizigus marmoreus can be increased greatly; production cost is effectively reduced; stable quality isguaranteed; and a popularization prospect is achieved.

The invention discloses a method for preparing high-quality ganoderma lucidum compost. The method for preparing the high-quality ganoderma lucidum compost includes the steps of mixing white mulberry branch chips, bran, white sugar and gypsum, packaging the mixture with a plastic bag, disinfecting the mixture through high-temperature steam, conducting inoculation after the temperature is lowered to the room temperature, conducting dark room cultivation, tearing apart the plastic bag when primordium appears in the fungus bag, filling gaps of fungus with fine earth, adjusting the brightness, the humiture and the ventilation condition in a greenhouse, harvesting ganoderma lucidum, packaging the dried ganoderma lucidum into bags, and finally placing and storing the packaged ganoderma lucidum into a warehouse. According to the method for preparing the high-quality ganoderma lucidum compost, the white mulberry branch chips are used as a main component of the ganoderma lucidum compost. The compost is low in price, materials are easy to obtain, the formula and the preparation method are simple, and the high-quality ganoderma lucidum is suitable for mass production. Therefore, according to the method for preparing the high-quality ganoderma lucidum compost, not only are down-stream products of silkworm production fully used, but also the industry chain of the silkworm production is extended, and an efficient method for improving the productivity and the quality of the ganoderma lucidum is found out.

The invention relates to a primordium control method during pleurotus eryngiu factory production. By controlling the moisture, the temperature, the illumination and the CO2 concentration, the primordium quantity of the pleurotus eryngiu is reduced with each bottle of 2 to 3, the yield of each bottle is between 130 and 150 grams, and the pleurotus eryngiu has large size, high quality and high commodity value. The primordium control method is free from using labor force to remove excess primordiums, reduces the labor force cost and the management cost of enterprises, and simultaneously avoids the reduction of product quality due to bacterial infection caused by human processing.

The invention discloses an uptight short-bag cultivation method for white needle mushroom. The method comprises the following steps: manufacturing a culture medium; mixing cottonseed hulls, waste cotton, corn powder, bran, lime and the like; adding water and evenly mixing; then, manufacturing a fungus bag; bagging; sterilizing; inoculating; culturing for mushroom emergence; carrying out fruiting management; processing mushroom stimulation; carrying out inducement to primordium; performing growth and development management of sporocarp; and finally, harvesting, and managing after harvesting. The uptight short-bag cultivation method has the advantages that uptight short-bag cultivation is adopted, so that the white needle mushroom stipe base has low adhesion, exuberant fruiting and excellent mushroom body, and biological efficiency is not less than 80%, excellent characters are achieved, the product quality of the white needle mushroom is improved, and yield is also improved.

The invention discloses a high-yield bagged material culture method of tea tree mushrooms, comprising the following steps of bagged material making, inoculation, mycelium culture and fruiting body culture. Bagged material has the advantages of scientific material matching and reasonable ratio and can supply essential nutrition to myceliums and fruiting bodies. The technological conditions of temperature, humidity and the like are controlled strictly in the culture process to accelerate mycelium growth and fruiting body primordium differentiation; thus, the invention has the advantages of good fruiting body development, high biological transformation rate, little misshapen mushroom production, uniform individual fruiting body, high quality, long mushroom production period, high yield, little pollution and low cost and further enhances the utilization ratio of cultural materials.

The invention discloses a pleurotus eryngii cultivation material which is characterized in that episperm of tea seeds and tea leaves are taken as main raw material, cane trash, wheat bran, oil-tea-cake, maize meal and the like are taken as auxiliary materials, and the water content of cultivation material can reach 63 percent to 65 percent. The invention further discloses a manufacturing method of the cultivation material. Compared with wood chip cultivation material, the cultivation material has the advantages as followings: firstly, the thickness matching of the cultivation material is reasonable, the fungus venting quality is excellent, the growth rate of pleurotus eryngii mycelium is fast, and the bag filling time is shortened; secondly, through natural accumulation and fermentation, substances such as xylogen, cellulose, hemicellulose, pectic substance and the like in the episperm of tea seeds and the tea leaves are resolved into simple substance which is convenient for the pleurotus eryngii mycelium to absorb, the formation of sporocarp primordium of the pleurotus eryngii is promoted, and the yield is increased by 25 percent to 30 percent; lastly, cultivation material channels are broadened, and the manufacturing cost is lowered greatly.

The invention relates to a culture medium for pleurotus eryngii and a cultivation method of the pleurotus eryngii. The culture medium comprises the following components according to the formula: 75 to 80 percent of solidago canadensis which is cut to have a particle diameter of 0.8 to 1.2cm, 17 to 22 percent of bran, 0.8 to 1.2 percent of sugar, 0.9 to 1.1 percent of gesso and 0.9 to 1.1 percent of lime. A cultivating material of which the water content is between 70 and 75 percent is prepared by uniformly stirring the components in the step (a) and then adding water into the mixture. The cultivation method of the pleurotus eryngii comprises the following steps of: preparing the cultivating material; putting the cultivating material of which the water content is between 70 and 75 percent into a cultivating bottle, and then sealing the bottle and sterilizing; opening sealing stamp and implanting a strain into the cultivating material of the cultivating bottle by using an inoculation tool; and carrying out inducement to primordium and fruiting management. The culture medium and the cultivation method of the invention have the advantages that not only the produced pleurotus eryngii has large size and good taste, but also the yield is high and the cultivation method is simple; the solidago canadensis is used as the main raw material of the culture medium, so that not only the solidago canadensis is changed profitable, but also the raw material source is wide, the price is low and the cost of producing the pleurotus eryngii is low; and the production environment is clean and free from peculiar smell, so that operators can work in the sanitary and clean environment.

The invention discloses a needle mushroom cultivating method. The needle mushroom cultivating method comprises the following steps: making bags, sterilizing, inoculating, performing mycelium culture and primordium inducement, opening the bags, cultivating regenerative mushrooms, and harvesting. Compared with the ordinary cultivating method, the needle mushroom cultivating method has the advantages of high yield and excellent quality; and the cultivated needle mushrooms have consistent color and luster, uniform thickness of stipes, basically consistent length and high commercial value.

The present invention discloses a north half-clinker open type mushroom production method, relates to a mushroom half-clinker production technology, especially relates to a north half-clinker open type mushroom production method, and pertains to the field of edible fungus production. The present invention provides a north half-clinker open type mushroom production method with a low cost, a simple operation and a high yield rate. Production method of the present invention comprises steps of strain manufacture, nutriment formula, disinfection, bagging (covering under soil) inoculation, mycelia culturing, colouring, inducement to primordium fruiting, harvesting and processing.

The invention discloses a method for planting high quality rice. The method comprises the steps of seed selection, seeding, rice transplanting, a panicle primordium differentiation stage, a heading period and a mature period. When rice ears fall and are golden, the rice can be reaped. Compared with the existing rice planting technology, the method has significant advantages.When rice planting is performed according to the technical scheme, the reaped rice produces full ears, the rice quality and quantity are improved greatly, the germination rate of rice seeds can be improved through the treatment of the rice seeds, fertilizer for rice is applied in the panicle primordium differentiation stage, and the fertilizer for the rice are added to fertilizer through conjugation reaction of bentonite, sodium carbonate and ferrous sulfate, so that the fertilizer efficiency is improved, the soil is improved, the absorption of the rice for the fertilizer is promoted, the rice can enter into the vegetative growth stage and reproductive growth stage side by side, and the vegetative reproduction of the rice is guaranteed to lay a solid foundation for head sprouting. According to the five-year continuous planting experiment statistics, the yield of the rice planting through the method is 18% to 20% higher than the yield of the rice planted by general planting methods.

A Japanese Grifola frondosa cultivating method specifically comprises steps as follows: selecting wood chips of broad-leaf species as a main cultivation material, and selecting wheat bran and Indian meal as nutrition sources; and then performing material mixing, bagging sterilization, inoculation, cultivation management, fruiting management and harvesting according to routine methods. During the cultivation management process, the temperature in a cultivate chamber is maintained to be 22-23 DEG C, the humidity is 60%-70%, and when white hypha lumps are formed on the surface of a fungus bag, gray primordium are formed and transparent water droplets are formed on the surface, 50 Lux lighting stimulation is required; and in a fruiting management process, when all the primordiums change into black and the water droplets are reduced, a ventilation silicone window at the upper part of the bag is required to be cut open by a cutter blade promptly, meanwhile, the temperature of the fruiting chamber is maintained to be 16-18 DEG C, the humidity is above 95%, and the lighting condition is maintained to be 500 Lux. With the implementation of the Japanese Grifola frondosa cultivating method, the condition that the precedent that industrial cultivation of the Grifola frondosa inland is not started yet at present is broken through, further, the production efficiency is quite high, and the market benefits are quite good.

The invention provides shiitake mushroom compost and a planting method of shiitake mushrooms, belongs to the technical field of vegetable planting and particularly relates to a planting method of the shiitake mushrooms. The planting method of the shiitake mushrooms comprises steps as follows: 1, arrangement of sowing time and selection of strains: medium-temperature-type or moderate-to-high-temperature strains are adopted; 2, preparation of the compost: the compost is a matrix and a material basis for growth and development of the shiitake mushrooms; different kinds of compost are adopted for production of the shiitake mushrooms due to the fact that organic substance resources in different places are different; firstly, gypsum and wheat bran are uniformly mixed and then uniformly mixed with wood flour, sugar and urea are dissolved in water and uniformly sprayed on the materials, the materials are turned over with a shovel while the mixture of the sugar and the urea are sprayed, and material surfaces are swept repeatedly and uniformly through a bamboo broom; 3, a nutrient growth state is switched to a reproductive growth state quickly under the irritation of certain conditions, and primordium differentiation and growth development of sporocarp are realized, that is, the fruiting period is started.

The present invention is based on the discovery that when a tooth primordium is inserted into a mammalian jaw and tooth formation follows, new alveolar bone around the new tooth forms. The present invention is based on the idea of stimulating new tooth formation via the implantation of tooth primordia at selected places in the jaw (e.g. at four points in the molar regions) to result in the formation of bony protuberances which could facilitate denture retention. The teeth may then be removed from the jaw of the patient to leave the new alveolar bone.

The invention relates to a stem tip detoxification and cultivation method for a sweet potato virus seedling. The method comprises the following steps: (1) taking a sweet potato block infected by sweet potato feathery mottle virus and / or sweet potato G virus; cultivating the sweet potato block indoors to sprout; (2) shearing scissoring terminal buds growing to 1.5-2.0 cm for disinfection; (3) peeling off the stem tips with two leaf primordiums in a sterile manner among the terminal buds as explants for vaccination cultivation to obtain regeneration plants; (4) conducting 2-3 times of successive transfer cultivation on the regeneration plants obtained in the step (3) to build a strain; conducting inspection by adopting an indicator plant method and a cellulose nitrate membrane ELISA in sequence to obtain a detoxified strain; (5) proliferating the detoxified strain quickly to obtain the detoxification seedling. The method can quickly build cultivation system suitable for stem tip cultivation and rapid propagation detoxification seedlings of majority of sweet potato varieties / strains.