201 results about "Hot start" patented technology

System, including methods, apparatus, compositions, and kits, for making and using compound droplets of a multiple emulsion to supply an amplification reagent, such as a heat-stable DNA polymerase or DNA ligase, to an aqueous phase in which the compound droplets are disposed. The compound droplets may be induced to supply the amplification reagent by heating the multiple emulsion, to achieve hot-start amplification.

An active set algorithm exploits a ‘hot start’ for the set of binding constraints at optimality along with efficient linear algebra to make rapid progress towards the solution. The linear algebra is designed to deal with degenerate constraints as the required factorizations are performed and as degeneracy emerges, and not via a mostly unnecessary pre-process step. Combined together, these novel approaches enable solution of the control problem in real-time.

The present invention is directed to a new composition for performing a nucleic acid amplification reaction comprising (i) a thermostable DNA-Polymerase, (ii) a thermostable 3'-5' Exonuclease, and (iii) at least one primer for nucleic acid amplification with a modified 3' terminal residue which is not elongated by said thermostable DNA-Polymerase as well as methods for performing a PCR reaction using this composition. Furthermore, the method is directed to kits comprising such a composition.

After commanding an engine to stop, an electronic controller controls a variable working-angle control mechanism to increase the working angle of an intake valve in preparation for restarting of the engine. In the case of an automatic stop, which is followed by restarting of the engine in a relatively short time, the working angle of the intake valve is set to the maximum working angle of the variable working-angle control mechanism, which maximizing decompression. In a manual stop, it is necessary to allow for both hot start and cold start. Also, in the manual stop, a target working angle when the engine is stopped is set less than that of a case of the automatic stop. Thus, the starting characteristic is prevented from being degraded by increased friction of engine parts during a cold start, and knocking is prevented from occurring due to a high compression ratio in a cylinder during a hot start.

In a method for performing a hot start polymerase chain reaction, a reaction mixture including a primer, a template molecule, and a buffer—but no polymerase enzyme—is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A polymerase enzyme is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the hot start polymerase chain reaction is performed. In a method for performing a temperature mediated reaction, a first component of the temperature mediated reaction is loaded in a reaction channel of a microfluidic device. An electrical current is applied to heat the reaction channel. A second component of the temperature mediated reaction is delivered into the reaction channel, and the reaction channel is subjected to conditions such that the temperature mediated reaction is performed.

A fuel system (10) serves to supply fuel (18) to an internal combustion engine. The fuel system includes a reservoir (16) and a first fuel pump (20) whose input is connected to the reservoir (16). In addition, a second fuel pump (38) is provided, whose input is connected to the first fuel pump (20). Furthermore, at least one injection valve (50) is provided, which is connected to the second fuel pump (38) and can supply fuel at least indirectly to a combustion chamber. A leakage line (68) is provided between the second fuel pump (38) and the reservoir (16). In order to permit a reliable hot start of the engine with a simultaneously low strain on the components, the invention proposes that the leakage line (68) be provided with a valve device (70), which has a shutoff function (72) and a pressure relief function (74) that are connected in parallel with each other.

Embodiments of the invention disclose an application cold start acceleration method and apparatus. The method is characterized by comprising the steps of decomposing an initialization task during cold start of an application into a real-time task and a delay task, wherein the real-time task is a task required to be initialized during hot start of the application, and the delay task is a task not required to be initialized during hot start of the application; executing the real-time task during cold start of the application; and delaying the execution of the delay task. According to the technical scheme, the initialization task during cold start of the application is decomposed into the real-time task and the delay task, the real-time task is executed during cold start of the application, and the execution of the delay task is delayed, so that by decomposing the initialization task in the cold start process of the application, it is ensured that on the premise that the application can normally run after being started, the cold start speed of the application is increased to the maximum extent, and the good usage experience is provided for a user.

A controlled start up technique may eliminate the excessive peak junction temperature by controlling the speed of the electric device. Lower speed may result in reduced power to the load. Reduced power may result in reduced losses to maintain reliable operating junction temperatures. Once the junction reaches the predetermined temperature limit, speed may be controlled to hold the junction temperature constant. As time elapses, the coolant temperature may reduce, thereby allowing a higher power level without an increase in IGBT temperature. Unlike conventional methods which may allow for full power continuously upon start up, thereby either potentially causing a high temperature shut-down condition or requiring additional thermal inertia to handle a high heat load due to a continuous full-power start up, the controlled start up eliminates the design penalties for extensive thermal enhancements to accommodate the occasional extreme hot start up.

The invention relates to a GPS rapid hot start method. After being started up by hot start, a receiver utilizes the assist of a local real time clock unit RTC (Real Time Clock) to carry out two-dimension trap of spreading code, i.e. carrier frequency when in a trap state, basically aligns local C/A codes with the received code flow with the deviation within a 0.5 code chip; the deviation between the carrier frequency and the received carrier frequency is not more than 100Hz; once signals are trapped, the bit synchronization is then carried out to find the telegraph bit boundary of 20 ms; once finding telegraph bit boundary is completed, the tracking state is entered in, so that a local spreading code generator and a local carrier generator track the received GPS signals and carrier signals in real time to complete first rapid positioning and directly enter into a positioning resolving link without waiting for the completion of a baseband subframe synchronous process to calculate the on-orbit position and the pseudo-range of a satellite, and resolve the receiver position through a least square method to complete the rapid positioning. The method further ensures the effectiveness through a hot start failure detection mechanism.

The invention relates to compositions, methods, and kits for hot start polynucleotide synthesis, including extension of primed polynucleotide templates and polymerase chain reaction (PCR). Hot start is provided by a thermally inactivated blocking polymerase protein that binds primed polynucleotide templates and prevents their access to a thermostable nucleic acid polymerase. High temperatures employed in the synthesis reaction cause the blocking polymerase to denature, thereby permitting the action of a thermostable processive polymerase. Compositions of the invention include a specific blocking polymerase protein which is a mutant of the Klenow fragment of E. coli DNA polymerase. The mutant is essentially devoid of polymerase activity, processivity, and 3′ to 5′ exonuclease activity. Use of the thermally inactivated blocking polymerase together with a thermostable polymerase reduces non-specific priming and accumulation of unwanted amplification products, increasing the specificity and sensitivity of the synthesis reaction.

The present invention concerns in general PCR reaction mixtures comprising a mixture of hot-start primers and non-hot-start primers for a given target sequence, including multiplex PCR reaction mixtures; methods utilizing same for detection of one or more target polynucleotide sequences; and kits comprising same.

Preferred embodiments of the present hot start lever mount permit the hot start control lever to be mounted in a selected one of a plurality of possible orientations on a vehicle handlebar. For example, the hot start lever may be mounted on either the right-hand side or left-hand side of the handlebar assembly, as desired by the individual user. In addition, preferred hot start levers may be mounted on either side of the handlebar axis, such as above or below the handlebar axis, or forward or rearward of the handlebar axis. In addition, preferably the hot start lever mount is incorporated with a standard control lever perch, such as a clutch lever perch or brake lever perch to efficiently utilize the available mounting space on the vehicle handlebar.

An oxygen sensor heater control determines heater activation based on an open-loop control parameter and a correction factor that compensates for part-to-part variability. Following a cold start where the heater temperature can be reliably estimated, the engine controller predicts the resistance of the heating element and heater circuit at the desired operating temperature of the sensor, and computes the correction factor for heater activation based on the predicted resistance values and nominal resistance values. At least one predicted value is stored in non-volatile memory, and used to compute the correction factor following a warm or hot start of the engine.

The invention belongs to the technical field of engine hot-starting design, and relates to a correcting method and device for aviation engine hot-starting oil supply. The method comprises the following steps of S1, acquiring an engine exhaust temperature EGT and an engine inlet temperature T2, and determining a difference value between the engine exhaust temperature EGT and the engine inlet temperature T2; S2, giving a correction coefficient associated with the difference value if the difference value is larger than a first set value; and S3, correcting a reference starting oil-gas ratio according to the correction coefficient to obtain a hot-starting oil-gas ratio, and controlling the fuel supply amount according to the heat-starting oil-gas ratio. According to the method, the engine parameters are monitored to automatically correct the oil supply of the engine starting process, and the problem that the engine hot starting exhaust temperature is too high is effectively solved; and thetemperature of an engine is not required to be reduced before hot-starting, so that the operation process of a user is simplified, and the labor and the material cost of the user are saved.

The invention belongs to the biotechnology field, and in particular relates to sample conditioning fluid based on denaturation precipitation as well as application thereof and a nucleic acid releasing method. The sample conditioning fluid comprises the components in percentage by mass: 1-15 percent of PEG400, 0.1-2 percent of surfactant, 1-20 percent of solid phase resin chelex-100, 3-20 percent of polymerase chain reaction (PCR) enhancer, 10-40 mmol/L of potassium hydroxide and 100-400 mmol/L of potassium chloride (KCL), wherein the solvent of the conditioning fluid is sterile water. The sample conditioning fluid is used for removing substances inhibiting PCR amplification based on denaturation precipitation, and the treated product can be subjected to direct amplification so as to guarantee the amplification stability; because of non-selectivity of the treated product based on the principle on the amplification, the fluid is suitable for taq enzymes, chemically-decorated and antibody-decorated hot start enzymes and the like and is particularly suitable for performing amplification on long sections and gene sections with complicated structure.

An engine preheating system supports starting industrial and/or diesel engines in cold weather, eliminating the need for cold weather idling. The system preheats the engine coolant, bringing the entire machine up to a warm starting condition. This provides good lubrication and immediate heat availability. The system is fully portable and does not rely on commercial power, operable at any location. The system utilizes propane fuel, which is widely available. The propane fuel burns quietly and cleanly as opposed to fuel oil. The system includes numerous safety features, such as flow indicators and modern microprocessor technology to ensure safe operation.

The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures.