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12 results about "Processivity" patented technology
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In molecular biology and biochemistry, processivity is an enzyme's ability to catalyze "consecutive reactions without releasing its substrate". For example, processivity is the average number of nucleotides added by a polymerase enzyme, such as DNA polymerase, per association event with the template strand. Because the binding of the polymerase to the template is the rate-limiting step in DNA synthesis, the overall rate of DNA replication during S phase of the cell cycle is dependent on the processivity of the DNA polymerases performing the replication. DNA clamp proteins are integral components of the DNA replication machinery and serve to increase the processivity of their associated polymerases. Some polymerases add over 50,000 nucleotides to a growing DNA strand before dissociating from the template strand, giving a replication rate of up to 1,000 nucleotides per second.
Improved thermostable viral reverse transcriptase
The present invention provides novel engineered reverse transcriptase enzymes that afford beneficial improvements in thermal stability, processivity, cDNA yields and elimination of secondary enzymatic activity. The present invention also provides methods for amplifying template nucleic acids using such reverse transcriptase enzymes. This invention addresses deficiencies in the current state of the art reverse transcriptase enzymes in RNA detection and analysis including deficiencies in detection sensitivity, specificity, side enzyme activities, enzyme stability and synthesis capacity, especially when using template nucleic acids ranging in length, secondary structure and nucleotide content.
Owner:QIAGEN BEVERLY LLC
Thermostable viral reverse transcriptase
The present invention provides novel engineered reverse transcriptase enzymes that afford beneficial improvements in thermal stability, processivity, cDNA yields and elimination of secondary enzymatic activity. The present invention also provides methods for amplifying template nucleic acids using such reverse transcriptase enzymes. This invention addresses deficiencies in the current state of the art reverse transcriptase enzymes in RNA detection and analysis including deficiencies in detection sensitivity, specificity, side enzyme activities, enzyme stability and synthesis capacity, especially when using template nucleic acids ranging in length, secondary structure and nucleotide content.
Owner:QIAGEN BEVERLY LLC
Exonuclease activity-weakened nucleic acid polymerase mutant for single-molecule sequencing
The present invention provides a polymerase mutant applicable to single-molecule sequencing, which has weakened 3'→5' exonuclease activity relative to a wild-type polymerase, while substantially maintaining or even improving the extension processivity, sequencing accuracy, and read length. The present invention also provides use of the polymerase mutant in single-molecule sequencing.
Owner:GENEUS TECH CHENGDU CO LTD
Methods and compositions to detect chemical adducts on oligonucleotides
A reverse transcription (RT) assay to directly detect chemical adducts on RNA. A fluorescence quenching assay to detect RT polymerization was optimized and employed to detect N 1-alkylation of inosine, an important post-transcriptional modification, using a phenylacrylamide as a model compound. The methods and composition may be expanded to identify novel reagents that form adducts with RNA, regardless of the primary sequence, and further explored to understand the relationship between RT processivity and natural post-transcriptional modifications in RNA.
Owner:RGT UNIV OF CALIFORNIA
Engineered polymerases for improved sequencing by binding
Provided herein are engineered DNA polymerases comprising modifications improving accuracy and processivity of the polymerase including modifications in the Motif A region, optionally, along with additional modifications in the palm and / or exonuclease domains of the polymerase. Also provided are nucleic acids encoding the engineered DNA polymerases comprising modifications in motif A of the polymerase, optionally, with additional modifications. Methods, vectors, kits, and compositions comprising the nucleic acids and compositions, methods and kits comprising the engineered polymerases are also provided.
Owner:PACIFIC BIOSCIENCES OF CALIFORNIA INC
Creation of low-lignin content bmr mutant of sorghum and its application
This invention discloses the creation of a low-lignin-content sorghum BMR mutant gene and its application, belonging to the field of genetic engineering technology. The BMR mutant gene includes (1) bmr6-m, which is obtained by site-directed mutagenesis of the SbCAD2 / BMR6 gene as shown in SEQ ID NO.1, with the nucleotide sequence shown in SEQ ID NO.20; and (2) bmr12-m, which is obtained by site-directed mutagenesis of the SbCOMT / BMR12 gene with the nucleotide sequence shown in SEQ ID NO.2, with the nucleotide sequence shown in SEQ ID NO.21. Based on the obtained sorghum BMR mutant gene, while reducing the lignin content of sorghum and weakening the biomass anti-degradation barrier, mutant materials without transgenic element insertion and with easily identifiable phenotypes are also obtained, laying the foundation for simultaneously improving the feed quality and industrial processing performance of sorghum.
Owner:INSTITUTE OF CROP SCIENCE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
Polymerase-template complexes
The present disclosure provides methods and compositions for enhancing the processivity of a polymerase in catalyzing template-dependent DNA synthesis in high concentrations of salt. Also disclosed are methods and compositions for enhancing the assembly of polymerase-template complex compatible with active DNA synthesis in the presence of low levels of nucleotides and at a high temperature, such as temperatures at or near the melting temperature of the polymerase.
Owner:ROCHE SEQUENCING SOLUTIONS INC
Engineered DNA polymerase and application thereof
The invention discloses an engineered DNA polymerase and an application thereof. In the first aspect, the invention provides the DNA polymerase, and the DNA polymerase has an amino acid sequence which is obtained by mutating the amino acid sequence from the first site to the 575th site as shown in SEQ ID NO.14 through at least one of A1)-A5). The DNA polymerase provided by the embodiment of the invention has the following beneficial effects that the DNA polymerase provided by the invention has one or more mutation sites including M8R, D12A, G197D, M97K, Q99D, N396R, D510Q, D510H, D510S, D510K, D510R, D510M, I504R, I504K and I504M, and the result shows that the sustainability of the polymerase is better when the polymerase is extended by using a non-natural substrate under a high-salt condition.
Owner:ANXUYUAN BIOTECHNOLOGY (SHENZHEN) CO LTD
Application of PtoNAC028 gene in regulating and controlling monomer proportion of poplar lignin
The invention discloses application of a PtoNAC028 gene in regulating and controlling the proportion of poplar lignin monomers, a gene coding region has a nucleotide sequence as shown in SEQ ID NO: 1, the proportion of the poplar lignin monomers can be increased or reduced by overexpressing or silently expressing the PtoNAC028 gene in a poplar body, and a key gene is provided for genetic improvement of poplar processing characters. The invention further discloses a method for cultivating the poplar with the high lignin monomer proportion, the PtoNAC028 gene is overexpressed in a poplar body, the lignin monomer proportion of the poplar can be remarkably increased, directional regulation and control of monomer lignin metabolism are achieved, and a new poplar variety which is good in wood property and fast in growing can be rapidly cultivated.
Owner:BEIJING FORESTRY UNIVERSITY
Recombinant reverse transcriptase with improved processivity
The present disclosure provides a recombinant reverse transcriptase with improved sustained synthesis capability and uses thereof. In one embodiment, the recombinant reverse transcriptase is a fusion protein comprising a reverse transcriptase and a cold shock protein.
Owner:FEIPENG LIFE SCIENCES CO LTD +1
Methods and compositions relating to a group ii intron reverse transcriptase
PendingUS20250270525A1Fusion with RNA-binding domainAntibody mimetics/scaffoldsMedicineReverse transcriptase
Disclosed herein are methods and compositions related to novel group II intron reverse transcriptases. These reverse transcriptases have been engineered to increase fidelity and / or processivity.
Owner:BOARD OF RGT THE UNIV OF TEXAS SYST
Two-step template switching reverse transcription methods
PCT designated stageWO2025212663A1Microbiological testing/measurementTransferasesGeneticsBiochemistry
Methods are described for enhancing complementary DNA (cDNA) synthesis from a ribonucleic acid (RNA) template during reverse transcription by an enzyme having template switching activity. The methods provide for enhancing the processivity of a reverse transcriptase.
Owner:YALE UNIVERSITY