The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection method of PRV (Porcine Rotavirus) inverse transcription and an application. The detection method comprises the following steps: (1) building a RT-LAMP reaction system, and setting a negative control at the same time, wherein the RT-LAMP reaction system is 25 [mu] L and comprises 2 [mu] L of template, 0.5 [mu]L of 0.8 [mu]M FIP primer, 0.5 [mu]L of 0.8 [mu]M BIP primer, 0.25 [mu]L of 0.2 [mu]M of F3 primer, 0.25 [mu]L of 0.2 [mu]M of B3primer,2[mu]L of dNTP (Diethyl-Nitrophenyl Thiophosphate), 5 [mu]M of MgSO4 (Magnesium Sulfate), 8UBst of DNA polymerase and 1*ThermoPol Buffer, and 1[mu]L of MLV (Murine Leukemia Virus) reverse transcriptase, and supplementing the volume to 25 [mu]L by sterile water; and the negative control template is sterile water; (2) after reacting for 20-60min at 61-65 DEG C in a constant temperature water bath boiler, terminating for 20 min at 80 DEG C, carrying out AGE (Agarose Gel Electrophoresis) identification on an amplification product or adding 2 [mu]L of SYBR Green dye, and observing a result in an UV lamp (Ultraviolet Lamp) or by naked eyes. According to the LAMP detection method provided by the invention, a convenient, fast and accurate molecular biological diagnosis method can be provided for the clinical diagnosis and epidemiological investigation of the PRV.