170 results about "Hapten" patented technology

The invention belongs to the technical field of biochemical engineering, and particularly relates to an amitriptyline hapten and a preparation method thereof, an amitriptyline antigen and a preparation method thereof, an antibody, a kit and application. The amitriptyline hapten has a structural formula shown as a formula (I) or a formula (II). Compared with an existing structure, the amitriptyline hapten prepared by the invention has a better immune effect. The obtained antibody is high in titer, strong in specificity and high in affinity, the LOD of amitriptyline by using an Elisa method established by the antibody is 0.013 ng / mL, the IC50 of amitriptyline is 0.865 ng / mL, the quantitative detection range is 0.07-470.3 ng / mL, the detection sensitivity is high, and the linear range is wide.

The invention discloses a test strip for detecting etoxazole and application of the test strip. The test strip comprises a sample absorption pad (1), a conjugate release pad (2), a reaction film (3), a water absorption pad (4) and a bottom plate (7), the reaction film is provided with a detection line (5) coated with an etoxazole hapten-carrier protein conjugate and a quality control line (6) coated with a goat anti-mouse antibody, and the conjugate release pad (2) is sprayed with an etoxazole monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting residual etoxazole in vegetables and fruits by using the etoxazole test strip. The test strip provided by the invention has the characteristics of simplicity in operation, high sensitivity, high detection speed, low cost and the like, and is suitable for screening and on-site monitoring of a large number of samples.

The invention relates to a medetomidine hapten, a monoclonal antibody, a hybridoma cell strain and application thereof, and belongs to the technical field of immunodetection. A medetomidine complete antigen is obtained by synthesizing a medetomidine hapten, a mouse is used as an immune animal to prepare a hybridoma cell strain secreting a medetomidine monoclonal antibody, the hybridoma cell strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.46518. The medetomidine monoclonal antibody secreted by the hybridoma cell strain has better detection sensitivity and detection specificity to medetomidine, the IC50 value is 10.42 ng / mL, and the cross reaction rate to various medetomidine analogues is lt; the method can be applied to preparation of medetomidine detection products, and provides a reliable tool for immunodetection of medetomidine.

The invention provides synthesis of a T-2 toxin antigen and preparation and application of a monoclonal antibody. Relates to the technical field of biotoxin detection. The preparation method of the T-2 toxin antigen comprises the following steps: step 1, synthesizing an immunogen T-2-BSA by adopting a carbodiimide method, dissolving 2mg of T-2 adipic acid with 0.5 mL of dimethylformamide, and recording as a solution A; 5 mg of bovine serum albumin and 3 mg of EDC are dissolved with a 2-morpholine ethanesulfonic acid buffer solution with the pH being 5.5, and the solution is marked as a solution B; step 2, under the condition of stirring, dropwise adding the solution A into the solution B, stirring for 18 hours in a dark place at 4 DEG C, then dialyzing for 3 days by using a phosphate buffer solution, changing the solution once a day, centrifuging the dialysate for 10 minutes at 3,500 r / min, and discarding the precipitate; the adipic acid is coupled with the T-2 toxin through a chemical method, and the unique step enriches the structural characteristics of the hapten, so that the prepared T-2 toxin complete antigen is excellent in immune effect, can efficiently excite immune response of an organism, and shows high specificity and sensitivity.

To provide a method for solving problems related to the delivery of R2 polyhapten reagents, such as short delivery times or bubble collapse. [Solution] A novel and improved method for separating absorbance caused solely by the addition of assay reagents, and a novel and improved method for detecting and flagging problems, particularly those caused by the delivery of R2 polyhapten reagents, as well as apparatus and compositions for use herein, are provided.

The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.

This application relates to a detection reagent, kit, and method that simultaneously improves sensitivity and broadens the detection range in competitive immunoassay. The reagent includes a detection antibody bound to luminescent microspheres that specifically binds to analyte molecules in the sample, and two sets of labeled antigens containing the same competing antigen and labeled molecules of different molecular weights bound to it. The competing antigen competes with the analyte molecule for binding to the detection antibody, and the affinity of the competing antigen for binding to the detection antibody is higher than that of the analyte molecule for binding to the detection antibody. The technical solution of this application, by using two sets of labeled antigens with different affinities, ensures that the labeled antigens that play a dominant role in samples with varying concentrations of analyte molecules differ. This is suitable for competitive immunoassay of small molecule antigens or haptens in both low-concentration and high-concentration samples, and can broaden the detection range while ensuring high functional sensitivity.

To provide RPA compositions and methods for detecting the presence or absence of a target nucleic acid using a dual-hapten probe.SOLUTION: This disclosure relates to methods and compositions for detecting a target nucleic acid sequence using a dual-hapten probe. More specifically, the present disclosure relates to detection of a target nucleic acid sequence using recombinase polymerase amplification (RPA) and a dual-hapten probe. In some cases, the detection is on lateral flow strips.SELECTED DRAWING: Figure 4

The invention relates to a preparation method of an 8-hydroxyguanine (8-Hydroxyguanine) detection kit based on a chemiluminescence immunoassay technology. The 8-Hydroxyguanine (8-Hydroxyguanine) detection kit is used for detecting 8-Hydroxyguanine. As 8-hydroxyguanine is small in molecular weight and poor in immunogenicity, when a corresponding antibody is prepared, firstly, a proper derivative site needs to be selected, and a derived hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and high affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of an 8-hydroxyguanine hapten, preparation of an 8-hydroxyguanine complete antigen and an anti-8-hydroxyguanine antibody, a method for determining the concentration of 8-hydroxyguanine, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise design and chemical synthesis of 8-hydroxyguanine derivatives. The 8-hydroxyguanine derivative disclosed by the invention has a structure as shown in a formula (I),

The present disclosure provides compositions and methods for the detection and treatment of cancer. Specifically, the compositions of the present technology include novel DOTA-haptens that may be complexed with a radioisotope (e.g., 225Ac). Also disclosed herein are methods of the using the DOTA-haptens of the present technology in diagnostic imaging as well as pretargeted radioimmunotherapy.

The application discloses a kind of rice mycotic acid hapten and antigen preparation application, it is related to antigen preparation technical field, introduce sulfydryl in the 2 of rice mycotic acid, and obtain rice mycotic acid antigen by coupling with protein through connecting arm, rice mycotic acid hapten and antigen preparation application include the following specific flow: S1, the synthesis of rice mycotic acid hapten;S2, the preparation of rice mycotic acid immunogen;S3, the preparation of rice mycotic acid coating original;S4, the preparation of rice mycotic acid monoclonal antibody;S5, the preparation of rice mycotic acid immunofluorescence detection card;S6, rice mycotic acid detection in food;S7, detection limit experiment;S8, false positive rate, false negative rate experiment.The rice mycotic acid hapten retains the structural characteristics of rice mycotic acid, better exposes rice mycotic acid structural characteristics, improves the immunity and immune recognition ability of antigen, lays foundation for preparing specific, high sensitivity antibody, and improves its rapid detection ability.

The application discloses a kind of diflufenican hapten, antigen, antibody and its preparation method and application.The application prepares diflufenican hapten as shown in formula (I) and corresponding diflufenican antigen, and specific antibody for detecting diflufenican is prepared by diflufenican antigen, and further constructs the immune analysis method for directly detecting diflufenican pesticide residue, which is particularly suitable for on-site rapid detection, and can meet the needs of regulatory authorities and detection agencies formula (I).

This invention belongs to the field of immunology and discloses a hybridoma cell line and the preparation and application of its secreted monoclonal antibody that uniformly recognizes tylosin / tilmicosin. The monoclonal antibody hybridoma cell line is named DES-AOAA-14D5, with accession number CGMCC NO: 45303. This invention utilizes decarboxymethyl tylosin and oxycarboxymethyl hydroxylamine to synthesize a hapten, conjugates a carrier protein to prepare a complete antigen, immunizes mice, and, through cell fusion and screening techniques, prepares a hybridoma cell line that can secrete a monoclonal antibody that uniformly recognizes tylosin / tilmicosin. IC50 50 The effective concentrations were 1.59 and 1.72 ng / mL, respectively, with a cross-reactivity rate as high as 92.44%. An immunoassay method for the simultaneous and accurate detection of tylosin / tilmicosin in milk was established using this antibody. The cut-off value for both tylosin and tilmicosin was 16 ng / mL, and no cross-reactivity was observed with other structural analogs. The detection method of this invention shows promising application prospects.

The invention discloses a halogen modified hapten for enhancing antibody titer and affinity as well as a preparation method and application of the halogen modified hapten, and belongs to the technical field of biochemical engineering. According to the method, an amantadine structure is innovatively and chemically modified, various halogens such as fluorine, chlorine and bromine are introduced, the AMA halogen modified hapten shown in the formula (I) is formed, and the traditional'most similar 'design principle is broken through. Compared with an unmodified hapten, the hapten modified by halogen in the invention has the advantages that the titer of the polyclonal antibody on amantadine can be obviously improved by 3.4-14 times; wherein the haptens A5 and A6 can obviously improve the titer of the monoclonal antibody to amantadine by 3-18 times, and the affinity is improved by 10.8-16.1 times. The invention provides a new thought and a new method for the rational design of the hapten of the small molecule compound and the preparation of the high-affinity antibody, and the related halogen modification type and quantity strategy can also provide valuable reference for the design of the hapten of other small molecule compounds.

A nanoparticle vaccine composition includes a shell including one or more amphiphilic molecules and one or more hapten molecules, where at least one amphiphilic molecule is conjugated to the hapten molecule, and a substantially hydrophobic core including an immunogenic protein, a vaccine adjuvant, one or more inert biocompatible materials, or a combination of two or more thereof, where the core is encapsulated in the shell to form a nanoparticle.

This invention relates to a medemididine hapten, a complete antigen, and their preparation methods and applications. The structure of the medemididine hapten is shown in Formula I. The preparation method of the medemididine hapten includes: (1) substituting medemididine with ethyl 3-bromopropionate to obtain intermediate 2; (2) adding sodium hydroxide to deprotect the ester group to obtain medemididine hapten 3. The structure of the complete antigen is shown in Formula II. The preparation method of the complete antigen includes: coupling the medemididine hapten with a carrier protein to obtain the complete antigen. This invention discloses a method for preparing medemididine hapten and complete antigen. The synthesis steps are simple and effective, and it can be used in immunoassay. Among them, colloidal gold immunochromatography is simple to operate, has a short detection time, high specificity, and good reproducibility, which can meet the domestic demand for immunoassay of medemididine.

The present disclosure provides compositions and methods for the detection and treatment of cancer. Specifically, the compositions of the present technology include novel compounds that may be complexed with a radioisotope. Also disclosed herein are methods of the using the DOTA-haptens of the present technology in diagnostic imaging as well as pretargeted radioimmunotherapy.

The invention relates to preparation of a cyclic adenosine monophosphate (cAMP) detection kit by using a chemiluminescence immunoassay technology. Cyclic adenosine monophosphate is an important second messenger for cell signal transduction and has wide physiological functions. Cyclic adenosine monophosphate is small in molecular weight and poor in immunogenicity, so that when a corresponding antibody is prepared, a proper derivative site needs to be selected firstly, and a derived hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and strong affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of a cyclic adenosine monophosphate hapten, preparation of a cyclic adenosine monophosphate complete antigen and an anti-cyclic adenosine monophosphate antibody, a method for measuring the concentration of cyclic adenosine monophosphate, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise design and chemical synthesis of a cyclic adenylate derivative. The cyclic adenylic acid derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

Compositions, kits, and systems containing tolythiazine haptens, immunogens, and / or protein conjugates are disclosed. Methods of producing and using the tolythiazine haptens, immunogens, and conjugates are also disclosed.

The invention relates to a preparation method of a cyclic guanylic acid (Guanosine 3 ', 5'-cyclic guanylic acid, cGMP) detection kit based on a chemiluminescence immunoassay technology, and particularly relates to a preparation method of a cyclic guanylic acid (Guanosine 3 ', 5'-cyclic guanylic acid, cGMP) detection kit based on a chemiluminescence immunoassay technology. Cyclic guanylate is small in molecular weight and poor in immunogenicity. When a corresponding antibody is prepared, a proper isomer and a derivative site need to be selected firstly, and a derivative hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and strong affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of a cyclic guanylic acid hapten, a cyclic guanylic acid complete antigen, preparation of an anti-cyclic guanylic acid antibody, a method for measuring the concentration of cyclic guanylic acid, and composition and components of a reagent. The design and synthesis of the hapten mainly comprise design and chemical synthesis of a cyclic guanylate derivative. The cyclic guanylate derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

The present application relates to a hybridoma cell strain secreting benzyl chlorophenol monoclonal antibody and its application, and belongs to the technical field of immune detection. The present application synthesizes benzyl chlorophenol hapten, prepares benzyl chlorophenol complete antigen, and through the steps of mouse immune response and cell fusion, a hybridoma cell strain secreting benzyl chlorophenol monoclonal antibody is screened and named CBP-3D4, which is preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC NO.46222. The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity, the IC 50 value of the monoclonal antibody secreted by the hybridoma cell strain for benzyl chlorophenol is 97.741 ng / mL, the cross reactivity of the monoclonal antibody for various benzyl chlorophenol functional analogs is less than 1%, and the monoclonal antibody can be applied to the preparation of benzyl chlorophenol detection products to realize the rapid quantification of benzyl chlorophenol.

Conjugates comprising a viral targeting ligand (e.g., an influenza virus targeting ligand), a linker, and a plurality of haptens are described. Also described are pharmaceutical compositions comprising the conjugates and methods of using the conjugates to treat viral infections or prevent such infections.

The application discloses a kind of Gui Shiwei Chinese herbal medicine organophosphorus pesticide rapid detection method and detection kit, by the sample of Gui Shiwei medicine powder to be measured is extracted by acetonitrile solution containing acetic acid, salting-out stratification is taken supernatant;With colloidal gold labeled anti-organophosphorus pesticide small peptide antibody incubation, carry out competition immune response in the microwell plate coated with organophosphorus hapten-protein complex;After washing, the absorbance is determined at 450 to 550nm wavelength, calculate competition inhibition rate and compare with preset first threshold value to determine whether it is over standard;The method is simple and rapid;In addition, for the detection needs of different organophosphorus pesticides, by setting different organophosphorus hapten-protein complex coating area in the microwell plate, the simultaneous detection of multiple organophosphorus pesticides is realized, and the applicability of organophosphorus pesticide residue detection is further improved.

The application relates to a hapten for detecting ethoxyquin, a complete antigen and a use method thereof. The application discloses a hapten for detecting ethoxyquin, and then a complete antigen obtained by coupling ethoxyquin hapten with hemocyanin is used as an immunogen, and a complete antigen obtained by coupling ethoxyquin hapten with chicken egg white albumin is used as a coating agent. An indirect detection enzyme-linked immunoassay method for ethoxyquin is established. The half-inhibition concentration of the method for ethoxyquin is 0.96 ug / mL, the detection limit is 0.6 ug / mL, the linear detection range is 0.72 ug / mL-1.28 ug / mL, and the curve correlation coefficient is 0.996. The method is high in specificity and sensitivity, simple and fast, and has a wide application prospect in the detection of ethoxyquin residues in food. The method is very suitable for realizing rapid qualitative or quantitative detection of ethoxyquin in food by primary site personnel.

Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.

The invention discloses a test strip for detecting fluoroquinolones, sulfonamides, tetracyclines and trimethoprim drug residues in aquatic products and a test method, and belongs to the technical field of drug detection.The preparation method comprises the steps that trimethoprim hapten is synthesized; synthesizing a quinolone hapten, a sulfanilamide hapten and a tetracycline hapten; preparing an immunogen and a coating antigen; preparing a monoclonal antibody; preparing a gold-labeled antibody; and pre-treating and assembling. According to the invention, an integrated detection scheme of four types of medicines is constructed, so that residual detection of fluoroquinolones, sulfonamides, tetracyclines and trimethoprim in aquatic products can be synchronously completed, and different detection tools or batch detection for single type of medicines is not needed. In an actual detection scene of aquatic products, repeated steps of sample treatment and detection operation can be greatly reduced, waste of aquatic product samples caused by repeated sampling is avoided, meanwhile, the overall detection period is shortened, and the rapid quality control requirement of the aquatic products from fishing / breeding to circulation links is met.

The present disclosure describes a peptide scaffold for producing vaccines. The peptide scaffold includes a peptide that self-assembles into a hapten carrier (hC) includes amphipathic alpha-helices. The peptide includes heptad repeats following a specific pattern. The hC further includes hapten or an agent conjugated to it, and optionally the hC includes one or more T-cell epitopes at the N- and / or C-terminus of the one or more amphipathic alpha-helices. The present disclosure also describes compositions including immunogenic compositions including the hapten-hC or agent-hC conjugate.

The present application relates to the technical field of hapten, in particular to vitamin D3 carboxylic acid derivative, antigen and preparation method thereof. The structural formula of vitamin D3 carboxylic acid derivative is shown as follows: the vitamin D3 carboxylic acid derivative has excellent antibody binding activity, the vitamin D3 antigen coupled has high activity and inhibition rate. And the synthesis cost of the vitamin D3 carboxylic acid derivative is low, the cycle is short, and the yield is high.

Disclosed herein is an engineered bacterium comprising a chimeric nucleic acid comprising: a first nucleic acid encoding an ompA protein or a fragment thereof; and a second nucleic acid encoding a radiohapten binding protein. Further disclosed are methods of using these constructs in methods for pre-targeted radiotherapy and for treating tumors.

The present application relates to a hybridoma cell strain secreting clozapine and its metabolite monoclonal antibody, and belongs to the technical field of immune detection. The present application prepares a hapten by chemically synthesizing clozapine and its metabolite, and then prepares a complete antigen, and then screens a hybridoma cell strain secreting clozapine and its metabolite monoclonal antibody through animal immunization and cell fusion. The hybridoma cell strain LDP-2B10 provided by the present application has been preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No. 46233. The monoclonal antibody secreted by the hybridoma cell strain has good sensitivity and specificity, the IC 50 value of the monoclonal antibody for clozapine is 5.041 ng / mL, the IC 50 value of the monoclonal antibody for the metabolite N-desmethylclozapine is 5.524 ng / mL, and the IC 50 value of the monoclonal antibody for the metabolite clozapine-N-oxide is 5.551 ng / mL. The cross reactivity of the monoclonal antibody for various clozapine functional analogues is less than 1%, and the monoclonal antibody can be used for detecting clozapine in urine and blood drug concentration, and has practical application value.