24 results about "Immunochemistry" patented technology

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-PR recombinant rabbit monoclonal antibody and application thereof.The anti-PR recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 5. The invention also relates to a nucleotide sequence for coding the anti-PR recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, application of the anti-PR recombinant rabbit monoclonal antibody in a PR protein detection method or device, and the like. The PR recombinant rabbit monoclonal antibody disclosed by the invention has the characteristics of good specificity, strong positive signal and the like, so that scoring is easier in IHC staining, and cancer detection and distinguishing are more accurate.

The present invention provides a novel anti-Aβ antibody capable of detecting an Aβ related peptide, a method for measuring an Aβ related peptide in a biological sample by immunochemically using the above-mentioned novel anti-Aβ antibody, and a kit. A monoclonal antibody that recognizes an Aβ related peptide, and is produced by a hybridoma deposited under the accession number NITE BP-02998 at the NITE Patent Microorganisms Depositary of the National Institute of Technology and Evaluation. An antibody-immobilized carrier that includes a carrier and the monoclonal antibody defined in claim 1 bound to the carrier. A kit for measuring an Aβ related peptide, comprising the antibody-immobilized carrier.

The invention discloses a protease cleavable liposome and application thereof in treating colon cancer. The preparation method of the protease cleavable liposome comprises the following steps: synthesizing functional peptide from cancer homing peptide, protease cleavable protein fragments and lysine; the preparation method comprises the following steps: reacting a functional peptide with difunctional polyethylene glycol COOH-PEG2000-Mal, and covalently linking the obtained product with DSPE-NHS to obtain a protease cleavable material; and coupling the aPDL1 antibody to a protease cleavable material to obtain the protease cleavable liposome. The protease cleavable liposome can activate cytokines to kill tumor cells, so that effective endocytosis by colon cancer cells is realized, and an immune checkpoint blocking effect is achieved. Therefore, the protease cleavable liposome not only can be used as an effective delivery carrier for immune checkpoint blocking treatment, but also can be used as a universal platform for colon cancer immunochemistry combined treatment. The method has an important application value.

This invention relates to a hybridoma cell line that secretes a monoclonal antibody against azoxystrobin and its application, belonging to the field of immunochemistry. The hybridoma cell line of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46742, and classified as a monoclonal cell line. The monoclonal antibody secreted by the invented hybridoma cell line exhibits good detection sensitivity (IC50) against azoxystrobin. 50 With a value of 0.049 ng / mL, it can be used for the detection of antimicrobial residues.

This invention discloses a filter coating composition for fluorescence detection and its preparation method, relating to the fields of optical materials and biodetection technology. The composition is formulated by compounding optical functional powders, polymer substrates, dispersants, and additives in specific proportions, exhibiting high transmittance, narrow wavelength selectivity, excellent weather resistance and mechanical stability. It can accurately filter excitation light and stray light, improving the sensitivity and specificity of fluorescence detection. The filter coating composition and its preparation method for fluorescence detection also provide a simple, cost-effective manufacturing process suitable for large-scale production, particularly applicable to gene sequencing and immunochemistry. It addresses the problems of poor wavelength selectivity, weak stability, and high cost associated with traditional filters, making it suitable for fluorescence detection scenarios such as Alzheimer's disease biomarkers. Balancing accuracy, stability, and economy, it has broad application prospects in the field of in vitro diagnostic reagents.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-BRAF-V600E recombinant rabbit monoclonal antibody and application thereof, which comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application also relates to a nucleotide sequence, a recombinant plasmid or an expression vector, a preparation method and application of the anti-BRAF-V600E recombinant rabbit monoclonal antibody in a BRAF-V600E protein detection method or device. The BRAF-V600E recombinant rabbit monoclonal antibody of the 135K5D1 clone of the application is good in specificity, strong in positive signal and the like, and is easier to score in IHC staining, and is more accurate in detection and distinction of cancer.

This invention relates to a cicutine hapten, a hybridoma cell line, an antibody, and their applications, belonging to the field of immunochemistry. The hybridoma cell line, ISO 400, is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, on April 18, 2024, with accession number CGMCC No. 45918. The monoclonal antibody secreted by the cell line provided by this invention exhibits good detection sensitivity (IC50) for cicutine. 50 With a value of 9.69 μg / mL and high specificity, it enables rapid detection of coniine, providing raw materials for the immunoassay of coniine in hemlock and cosmetics, and has practical application value.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-PR recombinant rabbit monoclonal antibody and application thereof. The anti-PR recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4. The amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application further relates to a nucleotide sequence encoding the anti-PR recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method and application of the anti-PR recombinant rabbit monoclonal antibody in a PR protein detection method or device. The anti-PR recombinant rabbit monoclonal antibody has the characteristics of good specificity and strong positive signal, and is easier to score in IHC staining and more accurate in distinguishing cancer.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-GATA3 recombinant rabbit monoclonal antibody and application thereof.The anti-GATA3 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 8; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 9. The invention also relates to nucleotide sequences SEQ ID NO: 6 and SEQ ID NO: 7 for coding the anti-GATA3 recombinant rabbit monoclonal antibody, a nucleic acid molecule, an expression vector or recombinant plasmid, a host cell, a preparation method of the antibody, application of the antibody in a GATA3 protein detection method or device, and the like. The GATA3 recombinant rabbit monoclonal antibody disclosed by the invention has the characteristics of good specificity, strong positive signal and the like, so that scoring in IHC staining is easier, and cancer detection and distinguishing are more accurate.

The invention belongs to the technical field of molecular biomedicine, and particularly relates to a urinary tract epithelial cell marker and application thereof in diagnosis of urinary tract epithelial cell carcinoma. The molecular marker is cytoplasm thyroid hormone binding protein CRYM, and an immunochemical staining experiment proves that the cytoplasm thyroid hormone binding protein CRYM has the potential to serve as a single immunomarker to be used for distinguishing normal urinary tract epithelial cells / reactive hyperplastic urinary tract epithelial cells and urinary tract epithelial cancer cells. Therefore, the kit plays a key identification role in histopathologic diagnosis or urine noninvasive screening of urothelial carcinoma, and provides important technical support for clinical noninvasive diagnosis.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-CD20 recombinant rabbit monoclonal antibody and application thereof, wherein the anti-CD20 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application further relates to a nucleotide sequence encoding the anti-CD20 recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, and application of the anti-CD20 recombinant rabbit monoclonal antibody in a CD20 protein detection method or device. The application solves the problems that the binding specificity and sensitivity of the currently marketed CD20 antibody and CD20 protein molecules need to be improved, the recognition degree is poor in IHC staining, and the detection and differentiation of cancer are not conducive.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-ER recombinant rabbit monoclonal antibody and application thereof. The anti-ER recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 8. The amino acid sequence of the light chain variable region is shown as SEQ ID NO: 9. The application further relates to a nucleotide sequence encoding the anti-ER recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method and application of the anti-ER recombinant rabbit monoclonal antibody in an ER protein detection method or device. The 658G3A2 clone ER recombinant rabbit monoclonal antibody of the application is good in specificity, strong in positive signal and the like, and is easier to score in IHC staining and more accurate in cancer detection and differentiation.

The invention provides a hybridoma cell strain capable of secreting a tetramethyl rhodamine monoclonal antibody and application of the hybridoma cell strain, and relates to the technical field of immunochemistry. According to the invention, tetramethyl rhodamine and protein are connected to prepare a complete antigen, and a hybridoma cell strain secreting a monoclonal antibody is obtained through mouse immunization and cell screening, and the preservation number is CGMCC NO.46520. The monoclonal antibody secreted by the cell strain has good detection sensitivity and specificity to tetramethyl rhodamine, the IC50 value to tetramethyl rhodamine is 1.79 ng / mL, and the cross reaction rate to various tetramethyl rhodamine structural analogues is lt; therefore, a foundation is established for immunodetection of tetramethyl rhodamine, and the tetramethyl rhodamine immunodetection kit has a good application prospect.

The present application relates to a kind of amygdalin hapten, hybridoma cell strain, antibody and application, belong to the field of immunochemistry.The hybridoma cell strain DMN has been preserved in China Microbial Culture Collection Center CGMCC, address Beijing Chaoyang District Beichen West Road No. 1 Yard No. 3, Institute of Microbiology, Chinese Academy of Sciences, preservation date is April 18, 2024, and the preservation number is CGMCC No.45923.The monoclonal antibody secreted by the cell strain provided in the present application has good detection sensitivity (IC 50 Values are 8.65 ng / mL) and specificity, which can realize the rapid detection of amygdalin, provide raw materials for the immunodetection of amygdalin in amygdalin and products, and have practical application value.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-ER recombinant rabbit monoclonal antibody and application thereof.The anti-ER recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 8; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 9. The invention also relates to a nucleotide sequence for coding the anti-ER recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, an application of the anti-ER recombinant rabbit monoclonal antibody in an ER protein detection method or device, and the like. The ER recombinant rabbit monoclonal antibody cloned from the 658G3A2 has the characteristics of good specificity, strong positive signal and the like, so that scoring is easier in IHC staining, and cancer detection and distinguishing are more accurate.

The application provides an immunochemical luminescence kit for detecting a cancer marker HPV18 E7 protein, the kit comprising biotin-labeled antibody 1 and acridinium ester-labeled antibody 2; the antibody 1 is a rabbit-derived monoclonal antibody, comprising a heavy chain and a light chain; the antibody 1 heavy chain variable region comprises VHCDR1, VHCDR2 and VHCDR3 with the amino acid sequences shown in SEQ ID NO. 1-3; the antibody 1 light chain variable region comprises VLCDR1, VLCDR2 and VLCDR3 with the amino acid sequences shown in SEQ ID NO. 9-11; the antibody 2 is a rabbit-derived monoclonal antibody, comprising a heavy chain and a light chain; the antibody 2 heavy chain variable region comprises VHCDR1, VHCDR2 and VHCDR3 with the amino acid sequences shown in SEQ ID NO. 17-19; and the antibody 2 light chain variable region comprises VLCDR1, VLCDR2 and VLCDR3 with the amino acid sequences shown in SEQ ID NO. 25-27. The application can improve the test sensitivity, improve the detection rate of weak positive samples, realize accurate quantification of HPV18 E7 protein in cervical exfoliated cells, and the detection method of the application is simple in operation, fully automatic in the detection process, and can greatly improve the detection efficiency.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-GATA3 recombinant rabbit monoclonal antibody and application thereof. The anti-GATA3 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The amino acid sequence of the heavy chain variable region is shown as SEQ ID NO:8. The amino acid sequence of the light chain variable region is shown as SEQ ID NO:9. The application also relates to nucleotide sequences SEQ ID NO:6 and SEQ ID NO:7 encoding the anti-GATA3 recombinant rabbit monoclonal antibody, a nucleic acid molecule, an expression vector or a recombinant plasmid, a host cell, a preparation method of the antibody, and application of the antibody in a GATA3 protein detection method or device. The GATA3 recombinant rabbit monoclonal antibody of the application has the characteristics of good specificity and strong positive signal, and is easier to score in IHC staining, and is more accurate in distinguishing cancer.

Le réactif immunochimique d'identification et de quantification d'une substance protéique-test contenue dans un produit alimentaire est constitué par au moins un anticorps monoclonal dirigé contre une protéine-test contenue dans ledit produit alimentaire et dénaturée par un traitement thermique effectué à une température de traitement donnée, pendant une durée déterminée. Application dudit réactif à l'identification et à la quantification d'une protéine-test présente dans un produit alimentaire à tester.

The invention discloses an antibody targeting respiratory syncytial virus F protein and application thereof, the antibody can specifically bind to the respiratory syncytial virus F protein, has high binding activity, and can be used for developing related biological products, such as detection reagents and detection kits, especially products for respiratory syncytial virus detection and diagnostic products, and the like. The antibody provided by the invention can be used for various detection methods such as ELISA detection, immunochemiluminescence detection, immunofluorescence detection and the like, and has the advantages of wide application range, strong applicability and the like.

This invention relates to the fields of biotechnology and immunochemistry, specifically to an anti-OGT monoclonal antibody, its preparation method, and its applications. The monoclonal antibody comprises a heavy chain variable region and a light chain variable region; the heavy chain variable region includes three complementarity-determining regions, the amino acid sequences of which are shown in SEQ ID NO. 10~SEQ ID NO. 12; the light chain variable region includes three complementarity-determining regions, the amino acid sequences of which are shown in SEQ ID NO. 13~SEQ ID NO. 15. This anti-OGT monoclonal antibody can accurately identify OGT expression. Immunohistochemical detection of various tissues has shown that this monoclonal antibody can effectively detect OGT protein expression on tumor cells, and it can be applied to detection and screening fields such as immunohistochemistry (IHC), flow cytometry, and Western blotting.

The present invention relates to bioconjugates and labeled derivatives of the adducts formed by the reaction of patulin with benzene-1,2-dithiols or their salts. Said conjugates are suitable for the production of antibodies capable of recognizing the aforementioned patulin-(benzene-1,2-dithiols) adducts with high affinity and specificity. Likewise, the present invention also relates to the use of bioconjugates of patulin-(benzene-1,2-dithiols) adducts and of labeled derivatives thereof as assay antigens. Furthermore, the present invention also relates to immunochemical methods for the analysis of patulin, using the bioconjugates and labeled derivatives and antibodies of the invention, which include a previous step to quantitatively convert patulin into these adducts. This invention also provides a kit for analyzing patulin comprising a benzene-1,2-dithiol or its salts, antibodies against patulin-(benzene-1,2-dithiols) adducts, and assay antigens.

The present disclosure provides improved methods of processing a fecal sample by a human subject in which the fecal sample is collected at home and subsequently delivered to a medical diagnostics laboratory. As described herein, the means of collecting and processing the fecal sample can desirably reduce the number of steps undertaken by the human subject, including avoiding steps of removing or separating the fecal sample into multiple portions. Furthermore, the human subject does not have to perform an immunological test (e.g., a fecal immunochemical test (FIT) or an immunochemical fecal occult blood test (iFOBT)) at home.

The application provides an immunochemiluminescence kit for detecting a cancer marker HPV16 E7 protein, the kit comprising biotin-labeled antibody 1 and acridinium ester-labeled antibody 2; the antibody 1 is a mouse monoclonal antibody, comprising a heavy chain and a light chain; the amino acid sequence of the antibody 1 heavy chain is shown in SEQ ID NO: 17, and the amino acid sequence of the antibody 1 light chain is shown in SEQ ID NO: 18; the antibody 2 is a rabbit-derived monoclonal antibody, comprising a heavy chain and a light chain; the antibody 2 heavy chain variable region comprises VHCDR1, VHCDR2 and VHCDR3 with the amino acid sequences shown in SEQ ID NO. 1-3; and the antibody 2 light chain variable region comprises VLCDR1, VLCDR2 and VLCDR3 with the amino acid sequences shown in SEQ ID NO. 9-11. The application can improve test sensitivity, improve the detection rate of weak positive samples, identify early cervical cancer, realize accurate quantification of HPV16 E7 protein in cervical exfoliated cells, and has the advantages of simple operation, automatic detection process and greatly improved detection efficiency.

The present disclosure provides improved methods of processing a fecal sample by a human subject in which the fecal sample is collected at home and subsequently delivered to a medical diagnostics laboratory. As described herein, the means of collecting and processing the fecal sample can desirably reduce the number of steps undertaken by the human subject, including avoiding steps of removing or separating the fecal sample into multiple portions. Furthermore, the human subject does not have to perform an immunological test (e.g., a fecal immunochemical test (FIT) or an immunochemical fecal occult blood test (iFOBT)) at home.