73 results about "Immunochemistry" patented technology

The invention relates to a hybridoma cell strain VAND8, an anti-vanillin monoclonal antibody generated by the hybridoma cell strain VAND8 and application, and belongs to the field of immunochemistry. The hybridoma cell strain VAND8 provided by the invention can be used for preparing a high-titer vanillin-resistant monoclonal antibody, and the titer measured by an enzyme-linked immunosorbent assay (ELISA) method can reach 3.2 * 10 < 5 >; the vanillin-resistant monoclonal antibody provided by the invention is high in sensitivity and good in specificity, the 50% inhibition concentration IC50 on vanillin is 22.1 mu g / mL, and the cross reaction rate with vanillin structural analogues is low or even no cross reaction exists; the method can be used for measuring the content of vanillin.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-PR recombinant rabbit monoclonal antibody and application thereof.The anti-PR recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 5. The invention also relates to a nucleotide sequence for coding the anti-PR recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, application of the anti-PR recombinant rabbit monoclonal antibody in a PR protein detection method or device, and the like. The PR recombinant rabbit monoclonal antibody disclosed by the invention has the characteristics of good specificity, strong positive signal and the like, so that scoring is easier in IHC staining, and cancer detection and distinguishing are more accurate.

The invention belongs to the technical field of immunochemiluminescence, and relates to a PAMAM-mediated labeled small molecule compound and a synthesis method and application thereof.The PAMAM-mediated labeled small molecule compound is composed of small molecules, PAMAM and a marker, and the small molecules are provided with active groups or provided with derivative groups capable of reacting with amino under certain activation conditions to form covalent binding; the marker is provided with a succinimide group. The difference between a coupling product and a small molecular structure is reduced to the maximum extent through PAMAM mediation, the PAMAM dosage is extremely small and stable, compared with PEG, the molecular weight is more uniform, non-specific binding caused by hydrophobic interaction can be greatly reduced in immunoreaction, and good signals and separation can be obtained without purification of the product.

The present invention provides a novel anti-Aβ antibody capable of detecting an Aβ related peptide, a method for measuring an Aβ related peptide in a biological sample by immunochemically using the above-mentioned novel anti-Aβ antibody, and a kit. A monoclonal antibody that recognizes an Aβ related peptide, and is produced by a hybridoma deposited under the accession number NITE BP-02998 at the NITE Patent Microorganisms Depositary of the National Institute of Technology and Evaluation. An antibody-immobilized carrier that includes a carrier and the monoclonal antibody defined in claim 1 bound to the carrier. A kit for measuring an Aβ related peptide, comprising the antibody-immobilized carrier.

The invention belongs to the technical field of immunochemiluminescence, and relates to a PAMAM-mediated antibody marker and a synthesis method and application thereof, the PAMAM-mediated antibody marker is composed of an antibody, PAMAM and a marker, amino groups on the surface of the antibody can be converted into sulfydryl groups, and the marker is provided with succinimide groups which can react with the amino groups. The steric hindrance of antigen-antibody reaction is reduced through PAMAM mediation, the labeling proportion is increased to the maximum extent, and a high coupling effect can be achieved with a small feeding proportion.

The present application relates to the technical field of biological medicine, and provides a thrombin-antithrombin III complex antibody, a detection kit and application thereof.The present application specifically discloses a thrombin monoclonal antibody and an antithrombin III monoclonal antibody pairing combination, an intermediate product and a detection kit product containing the same.The antibody combination of the present application can be combined with the thrombin-antithrombin III complex in a double-antibody sandwich method with high sensitivity and strong specificity;the thrombin-antithrombin III complex detection kit suitable for the immunochemiluminescence method is prepared by using the above antibody, and the thrombin-antithrombin III complex in the human body can be detected by a two-step method.

The present invention particularly provides devices, test kits, equipment and methods for rapid homogeneous cell staining and imaging. Specifically, in some embodiments, the present invention can be less than 60 seconds to carry out immunochemical staining of cells or tissues without the need for rinsing. In some embodiments, the present invention stains and observes intracellular analytes (proteins or nucleic acids) within 60 seconds without the need for rinsing.

The present invention relates to a combination preparation comprising: an interleukin-2 (IL-2) immune anticancer drug; and a compound represented by chemical formula 1, a stereoisomer thereof, a crystalline form thereof, or a pharmaceutically acceptable salt thereof, whereby the combination preparation can exhibit the effects of enhancing the efficacy of an interleukin-2 immune anticancer drug and alleviating vascular leakage syndrome, which is a side effect of the interleukin-2 immune anticancer drug. [Chemical formula 1]

The invention discloses a protease cleavable liposome and application thereof in treating colon cancer. The preparation method of the protease cleavable liposome comprises the following steps: synthesizing functional peptide from cancer homing peptide, protease cleavable protein fragments and lysine; the preparation method comprises the following steps: reacting a functional peptide with difunctional polyethylene glycol COOH-PEG2000-Mal, and covalently linking the obtained product with DSPE-NHS to obtain a protease cleavable material; and coupling the aPDL1 antibody to a protease cleavable material to obtain the protease cleavable liposome. The protease cleavable liposome can activate cytokines to kill tumor cells, so that effective endocytosis by colon cancer cells is realized, and an immune checkpoint blocking effect is achieved. Therefore, the protease cleavable liposome not only can be used as an effective delivery carrier for immune checkpoint blocking treatment, but also can be used as a universal platform for colon cancer immunochemistry combined treatment. The method has an important application value.

This invention relates to a hybridoma cell line that secretes a monoclonal antibody against azoxystrobin and its application, belonging to the field of immunochemistry. The hybridoma cell line of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46742, and classified as a monoclonal cell line. The monoclonal antibody secreted by the invented hybridoma cell line exhibits good detection sensitivity (IC50) against azoxystrobin. 50 With a value of 0.049 ng / mL, it can be used for the detection of antimicrobial residues.

This invention discloses a filter coating composition for fluorescence detection and its preparation method, relating to the fields of optical materials and biodetection technology. The composition is formulated by compounding optical functional powders, polymer substrates, dispersants, and additives in specific proportions, exhibiting high transmittance, narrow wavelength selectivity, excellent weather resistance and mechanical stability. It can accurately filter excitation light and stray light, improving the sensitivity and specificity of fluorescence detection. The filter coating composition and its preparation method for fluorescence detection also provide a simple, cost-effective manufacturing process suitable for large-scale production, particularly applicable to gene sequencing and immunochemistry. It addresses the problems of poor wavelength selectivity, weak stability, and high cost associated with traditional filters, making it suitable for fluorescence detection scenarios such as Alzheimer's disease biomarkers. Balancing accuracy, stability, and economy, it has broad application prospects in the field of in vitro diagnostic reagents.

The invention relates to the technical field of medicines, in particular to a nano delivery system for treating tumors through immune-chemical kinetics. The hyaluronic acid coated copper peroxide quantum dots are prepared by adopting a one-step method, and hyaluronic acid is used as a stabilizer and a dispersing agent, so that the stability of peroxide can be improved, and the active targeting property is also realized. Meanwhile, the introduction of copper peroxide starts a Fenton-like reaction, and activates a chemical kinetics therapy. In addition, the copper peroxide also reacts with an immunotherapy drug metformin to generate nitric oxide, so that blood vessel normalization is promoted, single-group drug induction is realized, and a pleasant effect of multiple treatments is triggered. In-vivo and in-vitro experimental results show that HMSN-Met-HA-CuO2 has good biological safety and stable nitric oxide release ability, has considerable targeting ability to 4T1 tumor cells, and can effectively inhibit the growth of a tumor model.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-BRAF-V600E recombinant rabbit monoclonal antibody and application thereof, which comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application also relates to a nucleotide sequence, a recombinant plasmid or an expression vector, a preparation method and application of the anti-BRAF-V600E recombinant rabbit monoclonal antibody in a BRAF-V600E protein detection method or device. The BRAF-V600E recombinant rabbit monoclonal antibody of the 135K5D1 clone of the application is good in specificity, strong in positive signal and the like, and is easier to score in IHC staining, and is more accurate in detection and distinction of cancer.

This invention relates to a cicutine hapten, a hybridoma cell line, an antibody, and their applications, belonging to the field of immunochemistry. The hybridoma cell line, ISO 400, is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, on April 18, 2024, with accession number CGMCC No. 45918. The monoclonal antibody secreted by the cell line provided by this invention exhibits good detection sensitivity (IC50) for cicutine. 50 With a value of 9.69 μg / mL and high specificity, it enables rapid detection of coniine, providing raw materials for the immunoassay of coniine in hemlock and cosmetics, and has practical application value.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-PR recombinant rabbit monoclonal antibody and application thereof. The anti-PR recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4. The amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application further relates to a nucleotide sequence encoding the anti-PR recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method and application of the anti-PR recombinant rabbit monoclonal antibody in a PR protein detection method or device. The anti-PR recombinant rabbit monoclonal antibody has the characteristics of good specificity and strong positive signal, and is easier to score in IHC staining and more accurate in distinguishing cancer.

Disclosed are an immunochemical assay device and a method of using the immunochemical assay device for detecting one or more targets or markers such as Cardiac Troponin I, NT-pro-BNP, D-dimer and / or cross-linked fibrin in a fluid sample.

The invention discloses an ochratoxin A hapten, an ochratoxin A artificial antigen, an ochratoxin A antibody and a preparation method and application thereof, and relates to the technical field of immunochemistry and biotoxin detection. The hapten is prepared from ochratoxin A and succinic anhydride through reaction, the artificial antigen is prepared from the hapten and isobutyl chloroformate, and the antibody is prepared from animals immunized by the artificial antigen. The antigen and the antibody can be applied to preparation of an ochratoxin A colloidal gold immunochromatography test strip, and can rapidly and sensitively detect ochratoxin A residues in animal-derived food.

The present invention relates to macromolecular blockers of non-specific interactions of an analyte and other components of the analytical system with the solid phase, which considerably decreases the non-specific interactions during the assay. The macromolecular blocker comprises synthetic copolymer or homopolymer, selected from the group comprising polyacrylamide, polymethacrylamide, polyacrylate, polymethacrylate, poly(N-(2-hydroxypropyl)methacrylamide), poly(2-oxazoline); to which at least one hydrophobically-active anchor is attached via a covalent bond, said hydrophobically-active anchor enabling suppression of non-specific interactions in immuno assays. The invention further relates to a method of preparing and to the use of said macromolecular blockers for capturing of specific antibodies or antigens or other molecules on the surface of the solid phase. The present invention enables to replace animal-derived proteins with a synthetic macromolecular blocker that is not only more effective in blocking activity itself, but it is also defined in its structure, has high batch-to-batch reproducibility, and does not need to be tested for the presence of viruses and other pathogens.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-GATA3 recombinant rabbit monoclonal antibody and application thereof.The anti-GATA3 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 8; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 9. The invention also relates to nucleotide sequences SEQ ID NO: 6 and SEQ ID NO: 7 for coding the anti-GATA3 recombinant rabbit monoclonal antibody, a nucleic acid molecule, an expression vector or recombinant plasmid, a host cell, a preparation method of the antibody, application of the antibody in a GATA3 protein detection method or device, and the like. The GATA3 recombinant rabbit monoclonal antibody disclosed by the invention has the characteristics of good specificity, strong positive signal and the like, so that scoring in IHC staining is easier, and cancer detection and distinguishing are more accurate.

The invention belongs to the technical field of molecular biomedicine, and particularly relates to a urinary tract epithelial cell marker and application thereof in diagnosis of urinary tract epithelial cell carcinoma. The molecular marker is cytoplasm thyroid hormone binding protein CRYM, and an immunochemical staining experiment proves that the cytoplasm thyroid hormone binding protein CRYM has the potential to serve as a single immunomarker to be used for distinguishing normal urinary tract epithelial cells / reactive hyperplastic urinary tract epithelial cells and urinary tract epithelial cancer cells. Therefore, the kit plays a key identification role in histopathologic diagnosis or urine noninvasive screening of urothelial carcinoma, and provides important technical support for clinical noninvasive diagnosis.

The invention relates to an apigenine hapten, a hybridoma cell strain, an antibody and application, and belongs to the technical field of immunochemistry. The hybridoma cell strain IS0 is preserved in China General Microbiological Culture Collection Center (CGMCC) in Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, on April 18, 2024, and has a preservation number of CGMCC No.45918. The hybridoma cell strain IS0 can be used for preparing a drug for preventing and treating tumors. The monoclonal antibody secreted by the cell strain provided by the invention has better detection sensitivity (the IC50 value is 9.69 mu g / mL) and specificity to the apigenine, can realize rapid detection of the apigenine, provides a raw material for immunodetection of apigenine in apigenin and cosmetics, and has a practical application value.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-CD20 recombinant rabbit monoclonal antibody and application thereof.The anti-CD20 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 5. The invention also relates to a nucleotide sequence for coding the anti-CD20 recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, an application of the anti-CD20 recombinant rabbit monoclonal antibody in a CD20 protein detection method or device, and the like. The problems that the binding specificity and sensitivity of the current commercially available CD20 antibody and CD20 protein molecules need to be improved, the identification degree in IHC dyeing is poor, and cancer detection and distinguishing are not facilitated are solved.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-CD20 recombinant rabbit monoclonal antibody and application thereof, wherein the anti-CD20 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application further relates to a nucleotide sequence encoding the anti-CD20 recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, and application of the anti-CD20 recombinant rabbit monoclonal antibody in a CD20 protein detection method or device. The application solves the problems that the binding specificity and sensitivity of the currently marketed CD20 antibody and CD20 protein molecules need to be improved, the recognition degree is poor in IHC staining, and the detection and differentiation of cancer are not conducive.

The invention relates to the technical field of biological medicine, and provides a thrombin-antithrombin III compound antibody, a detection kit and application of the thrombin-antithrombin III compound antibody. The invention specifically discloses a pairing combination of a thrombin monoclonal antibody and an antithrombin III monoclonal antibody, an intermediate product, a detection reagent product containing the pairing combination and the intermediate product, and the like. The antibody combination disclosed by the invention can be combined with a thrombin-antithrombin III compound with high sensitivity and strong specificity by a double-antibody sandwich method; a thrombin-antithrombin III compound detection kit suitable for an immunochemiluminescence method is prepared by using the antibody, and the thrombin-antithrombin III compound in a human body can be detected by a two-step method.

The invention belongs to the field of chemiluminescent magnetic bead preparation and detection, and particularly relates to a submicron magnetic bead and a preparation method and application thereof. Superparamagnetic ferroferric oxide microspheres are prepared by adopting a solvothermal method, the superparamagnetic ferroferric oxide microspheres are used as a template for surface modification and wrapping of a polymer shell layer, and finally carboxyl groups are modified, so that the carboxyl chemiluminescent magnetic beads are prepared. The preparation process is simple, convenient to operate and low in cost input, and the prepared carboxyl magnetic beads are high in stability, uniform in particle size, adjustable in particle size, adjustable in shell thickness, high in magnetic content, adjustable in magnetic content, fast in magnetic response and good in suspension property and have important application value in the field of immunobiochemistry.

The invention provides a joint detection kit for seven cardiopulmonary functions, and belongs to the technical field of immunochemical detection. The invention provides a joint detection kit for seven items of cardiopulmonary functions. The joint detection kit consists of a micro-fluidic chip, a quantitative blood collector, a disposable peripheral blood taking needle and a diluent, the micro-fluidic chip is composed of a PMMA base material chip, a blood filtering film and a cover plate. The micro-fluidic chip comprises a sample adding area, a marking reaction area, a time control valve and a micro-channel detection area. A synchronous detection scheme of seven indexes of the cardiopulmonary function is integrated, seven results can be output through one-time detection, high-sensitivity detection of seven targets is achieved, and the performance indexes such as accuracy, linearity and repeatability are excellent.

The utility model relates to the technical field of chemistry, in particular to a cell immunochemistry staining box which comprises a staining box body, a fixing assembly is arranged on the side surface of the staining box body, the fixing assembly comprises a first rectangular groove, and the first rectangular groove is formed in the side surface of the staining box body. According to the cell immunochemical staining box, by installing a fixing assembly, a rectangular test tube is inserted into a first rectangular groove, when the rectangular test tube makes contact with clamping plates, the rectangular test tube continues to be pushed inwards, the two clamping plates can be squeezed to the two sides till the rectangular test tube is completely inserted, and the rectangular test tube is fixed through resilience force of a spring; when the rectangular test tube is pulled out by the pull ring, the two clamping plates are reset through the resilience force of the spring, and the rectangular test tube is tightly fixed by the clamping plates, so that the cell environment in the test tube can be ensured not to be interfered by the outside if bumping occurs in the moving process; therefore, the possibility that the cells are damaged is greatly reduced.

The present invention provides a sulfoxaflor hapten, a hybridoma cell line, an antibody and its application, which belongs to the field of immunochemistry technology. The present invention provides a sulfoxaflor hapten, a complete antigen and a hybridoma cell line that secretes a sulfoxaflor monoclonal antibody, which has been deposited in the General Microbiology Center of the China Culture Collection of Microorganisms (CGMCC), the deposit address of which is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. It is classified and named as a monoclonal cell line, the deposit date is April 18, 2024, and the deposit number is CGMCC No. 45912. The monoclonal antibody secreted by this cell line has good detection sensitivity for sulfoxaflor and can be used for residual detection of sulfoxaflor.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-ER recombinant rabbit monoclonal antibody and application thereof. The anti-ER recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 8. The amino acid sequence of the light chain variable region is shown as SEQ ID NO: 9. The application further relates to a nucleotide sequence encoding the anti-ER recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method and application of the anti-ER recombinant rabbit monoclonal antibody in an ER protein detection method or device. The 658G3A2 clone ER recombinant rabbit monoclonal antibody of the application is good in specificity, strong in positive signal and the like, and is easier to score in IHC staining and more accurate in cancer detection and differentiation.

The invention discloses a H7 avian influenza virus antibody magnetic immunochemiluminescence detection kit and its application, belonging to the field of immunological detection. The kit contains enzyme-labeled antibodies, magnetic-labeled fluorescein isothiocyanate antibodies, fluorescein isothiocyanate modified antigens, standard solution, sample diluent, luminescent substrate liquid and concentrated washing solution; the antibody in the enzyme-labeled antibodies can specifically bind to the antigen in the fluorescein isothiocyanate modified antigen; the magnetic-labeled fluorescein isothiocyanate antibody is a core-shell structure; the core-shell structure is a fluorescein isothiocyanate antibody wrapped with magnetic nanoparticles; the luminescent substrate in the luminescent substrate liquid specifically binds to the enzyme in the enzyme-labeled antibody, so that the luminescent substrate emits fluorescence. The use of the kit to detect antibodies has the effects of high sensitivity, accuracy and strong specificity.