43 results about "Immunochemistry" patented technology

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-PR recombinant rabbit monoclonal antibody and application thereof.The anti-PR recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 5. The invention also relates to a nucleotide sequence for coding the anti-PR recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, application of the anti-PR recombinant rabbit monoclonal antibody in a PR protein detection method or device, and the like. The PR recombinant rabbit monoclonal antibody disclosed by the invention has the characteristics of good specificity, strong positive signal and the like, so that scoring is easier in IHC staining, and cancer detection and distinguishing are more accurate.

The present invention provides a novel anti-Aβ antibody capable of detecting an Aβ related peptide, a method for measuring an Aβ related peptide in a biological sample by immunochemically using the above-mentioned novel anti-Aβ antibody, and a kit. A monoclonal antibody that recognizes an Aβ related peptide, and is produced by a hybridoma deposited under the accession number NITE BP-02998 at the NITE Patent Microorganisms Depositary of the National Institute of Technology and Evaluation. An antibody-immobilized carrier that includes a carrier and the monoclonal antibody defined in claim 1 bound to the carrier. A kit for measuring an Aβ related peptide, comprising the antibody-immobilized carrier.

The present application relates to the technical field of biological medicine, and provides a thrombin-antithrombin III complex antibody, a detection kit and application thereof.The present application specifically discloses a thrombin monoclonal antibody and an antithrombin III monoclonal antibody pairing combination, an intermediate product and a detection kit product containing the same.The antibody combination of the present application can be combined with the thrombin-antithrombin III complex in a double-antibody sandwich method with high sensitivity and strong specificity;the thrombin-antithrombin III complex detection kit suitable for the immunochemiluminescence method is prepared by using the above antibody, and the thrombin-antithrombin III complex in the human body can be detected by a two-step method.

The invention discloses a protease cleavable liposome and application thereof in treating colon cancer. The preparation method of the protease cleavable liposome comprises the following steps: synthesizing functional peptide from cancer homing peptide, protease cleavable protein fragments and lysine; the preparation method comprises the following steps: reacting a functional peptide with difunctional polyethylene glycol COOH-PEG2000-Mal, and covalently linking the obtained product with DSPE-NHS to obtain a protease cleavable material; and coupling the aPDL1 antibody to a protease cleavable material to obtain the protease cleavable liposome. The protease cleavable liposome can activate cytokines to kill tumor cells, so that effective endocytosis by colon cancer cells is realized, and an immune checkpoint blocking effect is achieved. Therefore, the protease cleavable liposome not only can be used as an effective delivery carrier for immune checkpoint blocking treatment, but also can be used as a universal platform for colon cancer immunochemistry combined treatment. The method has an important application value.

This invention relates to a hybridoma cell line that secretes a monoclonal antibody against azoxystrobin and its application, belonging to the field of immunochemistry. The hybridoma cell line of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46742, and classified as a monoclonal cell line. The monoclonal antibody secreted by the invented hybridoma cell line exhibits good detection sensitivity (IC50) against azoxystrobin. 50 With a value of 0.049 ng / mL, it can be used for the detection of antimicrobial residues.

This invention discloses a filter coating composition for fluorescence detection and its preparation method, relating to the fields of optical materials and biodetection technology. The composition is formulated by compounding optical functional powders, polymer substrates, dispersants, and additives in specific proportions, exhibiting high transmittance, narrow wavelength selectivity, excellent weather resistance and mechanical stability. It can accurately filter excitation light and stray light, improving the sensitivity and specificity of fluorescence detection. The filter coating composition and its preparation method for fluorescence detection also provide a simple, cost-effective manufacturing process suitable for large-scale production, particularly applicable to gene sequencing and immunochemistry. It addresses the problems of poor wavelength selectivity, weak stability, and high cost associated with traditional filters, making it suitable for fluorescence detection scenarios such as Alzheimer's disease biomarkers. Balancing accuracy, stability, and economy, it has broad application prospects in the field of in vitro diagnostic reagents.

The invention relates to the technical field of medicines, in particular to a nano delivery system for treating tumors through immune-chemical kinetics. The hyaluronic acid coated copper peroxide quantum dots are prepared by adopting a one-step method, and hyaluronic acid is used as a stabilizer and a dispersing agent, so that the stability of peroxide can be improved, and the active targeting property is also realized. Meanwhile, the introduction of copper peroxide starts a Fenton-like reaction, and activates a chemical kinetics therapy. In addition, the copper peroxide also reacts with an immunotherapy drug metformin to generate nitric oxide, so that blood vessel normalization is promoted, single-group drug induction is realized, and a pleasant effect of multiple treatments is triggered. In-vivo and in-vitro experimental results show that HMSN-Met-HA-CuO2 has good biological safety and stable nitric oxide release ability, has considerable targeting ability to 4T1 tumor cells, and can effectively inhibit the growth of a tumor model.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-BRAF-V600E recombinant rabbit monoclonal antibody and application thereof, which comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application also relates to a nucleotide sequence, a recombinant plasmid or an expression vector, a preparation method and application of the anti-BRAF-V600E recombinant rabbit monoclonal antibody in a BRAF-V600E protein detection method or device. The BRAF-V600E recombinant rabbit monoclonal antibody of the 135K5D1 clone of the application is good in specificity, strong in positive signal and the like, and is easier to score in IHC staining, and is more accurate in detection and distinction of cancer.

This invention relates to a cicutine hapten, a hybridoma cell line, an antibody, and their applications, belonging to the field of immunochemistry. The hybridoma cell line, ISO 400, is deposited at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, on April 18, 2024, with accession number CGMCC No. 45918. The monoclonal antibody secreted by the cell line provided by this invention exhibits good detection sensitivity (IC50) for cicutine. 50 With a value of 9.69 μg / mL and high specificity, it enables rapid detection of coniine, providing raw materials for the immunoassay of coniine in hemlock and cosmetics, and has practical application value.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-PR recombinant rabbit monoclonal antibody and application thereof. The anti-PR recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4. The amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application further relates to a nucleotide sequence encoding the anti-PR recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method and application of the anti-PR recombinant rabbit monoclonal antibody in a PR protein detection method or device. The anti-PR recombinant rabbit monoclonal antibody has the characteristics of good specificity and strong positive signal, and is easier to score in IHC staining and more accurate in distinguishing cancer.

Disclosed are an immunochemical assay device and a method of using the immunochemical assay device for detecting one or more targets or markers such as Cardiac Troponin I, NT-pro-BNP, D-dimer and / or cross-linked fibrin in a fluid sample.

The invention discloses an ochratoxin A hapten, an ochratoxin A artificial antigen, an ochratoxin A antibody and a preparation method and application thereof, and relates to the technical field of immunochemistry and biotoxin detection. The hapten is prepared from ochratoxin A and succinic anhydride through reaction, the artificial antigen is prepared from the hapten and isobutyl chloroformate, and the antibody is prepared from animals immunized by the artificial antigen. The antigen and the antibody can be applied to preparation of an ochratoxin A colloidal gold immunochromatography test strip, and can rapidly and sensitively detect ochratoxin A residues in animal-derived food.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-GATA3 recombinant rabbit monoclonal antibody and application thereof.The anti-GATA3 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 8; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 9. The invention also relates to nucleotide sequences SEQ ID NO: 6 and SEQ ID NO: 7 for coding the anti-GATA3 recombinant rabbit monoclonal antibody, a nucleic acid molecule, an expression vector or recombinant plasmid, a host cell, a preparation method of the antibody, application of the antibody in a GATA3 protein detection method or device, and the like. The GATA3 recombinant rabbit monoclonal antibody disclosed by the invention has the characteristics of good specificity, strong positive signal and the like, so that scoring in IHC staining is easier, and cancer detection and distinguishing are more accurate.

The invention belongs to the technical field of molecular biomedicine, and particularly relates to a urinary tract epithelial cell marker and application thereof in diagnosis of urinary tract epithelial cell carcinoma. The molecular marker is cytoplasm thyroid hormone binding protein CRYM, and an immunochemical staining experiment proves that the cytoplasm thyroid hormone binding protein CRYM has the potential to serve as a single immunomarker to be used for distinguishing normal urinary tract epithelial cells / reactive hyperplastic urinary tract epithelial cells and urinary tract epithelial cancer cells. Therefore, the kit plays a key identification role in histopathologic diagnosis or urine noninvasive screening of urothelial carcinoma, and provides important technical support for clinical noninvasive diagnosis.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-CD20 recombinant rabbit monoclonal antibody and application thereof.The anti-CD20 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 5. The invention also relates to a nucleotide sequence for coding the anti-CD20 recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, an application of the anti-CD20 recombinant rabbit monoclonal antibody in a CD20 protein detection method or device, and the like. The problems that the binding specificity and sensitivity of the current commercially available CD20 antibody and CD20 protein molecules need to be improved, the identification degree in IHC dyeing is poor, and cancer detection and distinguishing are not facilitated are solved.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-CD20 recombinant rabbit monoclonal antibody and application thereof, wherein the anti-CD20 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4, and the amino acid sequence of the light chain variable region is shown as SEQ ID NO: 5. The application further relates to a nucleotide sequence encoding the anti-CD20 recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, and application of the anti-CD20 recombinant rabbit monoclonal antibody in a CD20 protein detection method or device. The application solves the problems that the binding specificity and sensitivity of the currently marketed CD20 antibody and CD20 protein molecules need to be improved, the recognition degree is poor in IHC staining, and the detection and differentiation of cancer are not conducive.

The invention relates to the technical field of biological medicine, and provides a thrombin-antithrombin III compound antibody, a detection kit and application of the thrombin-antithrombin III compound antibody. The invention specifically discloses a pairing combination of a thrombin monoclonal antibody and an antithrombin III monoclonal antibody, an intermediate product, a detection reagent product containing the pairing combination and the intermediate product, and the like. The antibody combination disclosed by the invention can be combined with a thrombin-antithrombin III compound with high sensitivity and strong specificity by a double-antibody sandwich method; a thrombin-antithrombin III compound detection kit suitable for an immunochemiluminescence method is prepared by using the antibody, and the thrombin-antithrombin III compound in a human body can be detected by a two-step method.

The invention belongs to the field of chemiluminescent magnetic bead preparation and detection, and particularly relates to a submicron magnetic bead and a preparation method and application thereof. Superparamagnetic ferroferric oxide microspheres are prepared by adopting a solvothermal method, the superparamagnetic ferroferric oxide microspheres are used as a template for surface modification and wrapping of a polymer shell layer, and finally carboxyl groups are modified, so that the carboxyl chemiluminescent magnetic beads are prepared. The preparation process is simple, convenient to operate and low in cost input, and the prepared carboxyl magnetic beads are high in stability, uniform in particle size, adjustable in particle size, adjustable in shell thickness, high in magnetic content, adjustable in magnetic content, fast in magnetic response and good in suspension property and have important application value in the field of immunobiochemistry.

The invention provides a joint detection kit for seven cardiopulmonary functions, and belongs to the technical field of immunochemical detection. The invention provides a joint detection kit for seven items of cardiopulmonary functions. The joint detection kit consists of a micro-fluidic chip, a quantitative blood collector, a disposable peripheral blood taking needle and a diluent, the micro-fluidic chip is composed of a PMMA base material chip, a blood filtering film and a cover plate. The micro-fluidic chip comprises a sample adding area, a marking reaction area, a time control valve and a micro-channel detection area. A synchronous detection scheme of seven indexes of the cardiopulmonary function is integrated, seven results can be output through one-time detection, high-sensitivity detection of seven targets is achieved, and the performance indexes such as accuracy, linearity and repeatability are excellent.

The present invention provides a sulfoxaflor hapten, a hybridoma cell line, an antibody and its application, which belongs to the field of immunochemistry technology. The present invention provides a sulfoxaflor hapten, a complete antigen and a hybridoma cell line that secretes a sulfoxaflor monoclonal antibody, which has been deposited in the General Microbiology Center of the China Culture Collection of Microorganisms (CGMCC), the deposit address of which is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. It is classified and named as a monoclonal cell line, the deposit date is April 18, 2024, and the deposit number is CGMCC No. 45912. The monoclonal antibody secreted by this cell line has good detection sensitivity for sulfoxaflor and can be used for residual detection of sulfoxaflor.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-ER recombinant rabbit monoclonal antibody and application thereof. The anti-ER recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 8. The amino acid sequence of the light chain variable region is shown as SEQ ID NO: 9. The application further relates to a nucleotide sequence encoding the anti-ER recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method and application of the anti-ER recombinant rabbit monoclonal antibody in an ER protein detection method or device. The 658G3A2 clone ER recombinant rabbit monoclonal antibody of the application is good in specificity, strong in positive signal and the like, and is easier to score in IHC staining and more accurate in cancer detection and differentiation.

The invention provides a hybridoma cell strain capable of secreting a tetramethyl rhodamine monoclonal antibody and application of the hybridoma cell strain, and relates to the technical field of immunochemistry. According to the invention, tetramethyl rhodamine and protein are connected to prepare a complete antigen, and a hybridoma cell strain secreting a monoclonal antibody is obtained through mouse immunization and cell screening, and the preservation number is CGMCC NO.46520. The monoclonal antibody secreted by the cell strain has good detection sensitivity and specificity to tetramethyl rhodamine, the IC50 value to tetramethyl rhodamine is 1.79 ng / mL, and the cross reaction rate to various tetramethyl rhodamine structural analogues is lt; therefore, a foundation is established for immunodetection of tetramethyl rhodamine, and the tetramethyl rhodamine immunodetection kit has a good application prospect.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-BRAF-V600E recombinant rabbit monoclonal antibody and application thereof.The anti-BRAF-V600E recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 4; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 5. The invention also relates to a nucleotide sequence for coding the anti-BRAF-V600E recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method of the anti-BRAF-V600E recombinant rabbit monoclonal antibody, application of the anti-BRAF-V600E recombinant rabbit monoclonal antibody in a BRAF-V600E protein detection method or device, and the like. The BRAF-V600E recombinant rabbit monoclonal antibody cloned from 135K5D1 has the characteristics of good specificity, strong positive signal and the like, so that scoring is easier in IHC staining, and cancer detection and distinguishing are more accurate.

The present application relates to a kind of amygdalin hapten, hybridoma cell strain, antibody and application, belong to the field of immunochemistry.The hybridoma cell strain DMN has been preserved in China Microbial Culture Collection Center CGMCC, address Beijing Chaoyang District Beichen West Road No. 1 Yard No. 3, Institute of Microbiology, Chinese Academy of Sciences, preservation date is April 18, 2024, and the preservation number is CGMCC No.45923.The monoclonal antibody secreted by the cell strain provided in the present application has good detection sensitivity (IC 50 Values are 8.65 ng / mL) and specificity, which can realize the rapid detection of amygdalin, provide raw materials for the immunodetection of amygdalin in amygdalin and products, and have practical application value.

The invention belongs to the technical field of immunochemistry, and particularly relates to an anti-ER recombinant rabbit monoclonal antibody and application thereof.The anti-ER recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and the amino acid sequence of the heavy chain variable region is shown as SEQ ID NO: 8; the amino acid sequence of the light chain variable region is as shown in SEQ ID NO: 9. The invention also relates to a nucleotide sequence for coding the anti-ER recombinant rabbit monoclonal antibody, a recombinant plasmid or an expression vector, a preparation method, an application of the anti-ER recombinant rabbit monoclonal antibody in an ER protein detection method or device, and the like. The ER recombinant rabbit monoclonal antibody cloned from the 658G3A2 has the characteristics of good specificity, strong positive signal and the like, so that scoring is easier in IHC staining, and cancer detection and distinguishing are more accurate.

The application provides an immunochemical luminescence kit for detecting a cancer marker HPV18 E7 protein, the kit comprising biotin-labeled antibody 1 and acridinium ester-labeled antibody 2; the antibody 1 is a rabbit-derived monoclonal antibody, comprising a heavy chain and a light chain; the antibody 1 heavy chain variable region comprises VHCDR1, VHCDR2 and VHCDR3 with the amino acid sequences shown in SEQ ID NO. 1-3; the antibody 1 light chain variable region comprises VLCDR1, VLCDR2 and VLCDR3 with the amino acid sequences shown in SEQ ID NO. 9-11; the antibody 2 is a rabbit-derived monoclonal antibody, comprising a heavy chain and a light chain; the antibody 2 heavy chain variable region comprises VHCDR1, VHCDR2 and VHCDR3 with the amino acid sequences shown in SEQ ID NO. 17-19; and the antibody 2 light chain variable region comprises VLCDR1, VLCDR2 and VLCDR3 with the amino acid sequences shown in SEQ ID NO. 25-27. The application can improve the test sensitivity, improve the detection rate of weak positive samples, realize accurate quantification of HPV18 E7 protein in cervical exfoliated cells, and the detection method of the application is simple in operation, fully automatic in the detection process, and can greatly improve the detection efficiency.

The application belongs to the technical field of immunochemistry, and particularly relates to an anti-GATA3 recombinant rabbit monoclonal antibody and application thereof. The anti-GATA3 recombinant rabbit monoclonal antibody comprises a heavy chain variable region and a light chain variable region. The amino acid sequence of the heavy chain variable region is shown as SEQ ID NO:8. The amino acid sequence of the light chain variable region is shown as SEQ ID NO:9. The application also relates to nucleotide sequences SEQ ID NO:6 and SEQ ID NO:7 encoding the anti-GATA3 recombinant rabbit monoclonal antibody, a nucleic acid molecule, an expression vector or a recombinant plasmid, a host cell, a preparation method of the antibody, and application of the antibody in a GATA3 protein detection method or device. The GATA3 recombinant rabbit monoclonal antibody of the application has the characteristics of good specificity and strong positive signal, and is easier to score in IHC staining, and is more accurate in distinguishing cancer.

Le réactif immunochimique d'identification et de quantification d'une substance protéique-test contenue dans un produit alimentaire est constitué par au moins un anticorps monoclonal dirigé contre une protéine-test contenue dans ledit produit alimentaire et dénaturée par un traitement thermique effectué à une température de traitement donnée, pendant une durée déterminée. Application dudit réactif à l'identification et à la quantification d'une protéine-test présente dans un produit alimentaire à tester.

The invention belongs to the field of immunodetection, and particularly relates to a combined double-staining immune cell chemical detection kit and application thereof, which can be used for screening and identifying HPV infection related and non-HPV infection related cervical cancer and precancerous lesions. Comprising a CLDN18.2 / Ki-67 primary antibody working mixed solution, a p16 / Ki-67 primary antibody working mixed solution, a goat anti-mouse / rabbit secondary antibody working mixed solution, a DAB working mixed solution, an RED working mixed solution and hematoxylin. According to the invention, liquid-based slide preparation is carried out on cervical exfoliated cells, vagina exfoliated cytology and the like, and CLDN18.2 / Ki-67 and p16 / Ki-67 are combined with immunochemical double staining, so that not only can cervical squamous carcinoma and precancerous lesions, but also cervical adenocarcinoma and precancerous lesions related to HPV infection be covered, and cervical adenocarcinoma and precancerous lesions related to non-HPV infection can be covered; and the kit has good specificity and sensitivity.

The present invention provides, inter alia, devices, kits, apparatuses and methods for rapid homogeneous cell staining and imaging. Specifically, in some embodiments, the present invention can immunochemically stain cells or tissues in less than 60 seconds without rinsing. In some embodiments, the present invention stains and observes an analyte (protein or nucleic acid) within a cell within 60 seconds without rinsing.