86 results about "Artificial antigen" patented technology

The invention discloses a phthalimidic acid hapten, an artificial antigen, an antibody, and a preparation method and application of the phthalimidic acid hapten and the artificial antigen. The structure of the phthalimide acid hapten is shown as a formula (I), and the phthalimide acid hapten is obtained by introducing a carboxyl active group with a spacer arm with a certain length to carboxyl of phthalimide acid, so that the phthalimide acid hapten can be coupled with carrier protein to obtain an artificial antigen for immunization; the phthalimide hapten retains all characteristic groups of the phthalimide, the original structural characteristics of the phthalimide are changed to the minimum extent, and a foundation is laid for generating an antibody with higher specificity and higher sensitivity for subsequently stimulating animal immune response. An artificial antigen and a phthalimide acid monoclonal antibody are prepared through the hapten, indirect competitive ELISA based on the monoclonal antibody has the lowest detection limit of phthalimide acid of 2.44 ng / mL, IC50 of 17.29 ng / mL and the linear range of 5.02-59.51 ng / mL, the detection sensitivity is high, and the linear range is wide.

The application discloses a kind of areca alkaloid artificial hapten, artificial antigen and its preparation method and application, with norarecoline hydrochloride as the starting material for synthesizing artificial hapten.This areca alkaloid artificial hapten retains the characteristic structure of areca alkaloid to the greatest extent, and has active groups that can couple with carrier proteins, which can be used as antigenic determinants;Further, the areca alkaloid artificial antigen obtained can be used to immunize to obtain an anti-areca alkaloid artificial antibody with high affinity, high sensitivity and strong specificity.The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:70000, and can be used for rapid and accurate immunodetection and immunoassay of areca alkaloid.

The invention discloses design and synthesis of a hapten of cyromazine, development of a cyromazine artificial antigen and a cyromazine antibody, and application of the cyromazine artificial antigen and the cyromazine antibody in the field of food safety rapid detection. The aminocarboxylic acid modified cyromazine hapten product is prepared through the steps of cyromazine hapten design, connecting arm introduction, synthesis, purification and the like. The cyromazine hapten with the structure is coupled with keyhole limpet hemocyanin to form an artificial immune antigen with immunogenicity, and is coupled with chicken ovalbumin to form an artificial competitive antigen. Tests show that the artificial immune antigen has high immunocompetence and can stimulate an animal immune system to generate an anti-cyromazine antibody, and the titer of an immune Blab / c mouse serum antibody is 16000. The IC50 of an enzyme-linked immunosorbent assay (ELISA) detection method established by the cyromazine artificial competitive antigen and the anti-cyromazine antibody is 5.3 [mu] g / L, and the detection sensitivity of an established gold-labeled immunochromatography detection method reaches 50 [mu] g / L.

The present invention relates to artificial antigen presenting cell (aAPC) scaffolds to provide cells with specific functional stimulation to obtain phenotypic and functional properties ideal to mediate tumor regression or viral clearance. In particular, the scaffolds of the present invention comprise stabilized MHC class I molecules free of antigenic peptide. The scaffolds can be loaded with antigenic peptide on demand, providing an agile platform for effective expansion and functional stimulation of specific T cells in a peptide-MHC-directed fashion.

The present application belongs to the technical field of bio-chemical industry, and particularly relates to a lorcaserin artificial hapten, a lorcaserin artificial antigen, and a preparation method and application thereof. The lorcaserin hydrochloride is used as a starting material for synthesizing the artificial hapten, the lorcaserin artificial hapten of the present application retains the characteristic structure of lorcaserin to the greatest extent, and has an active group which can be coupled with a carrier protein, and can be used as an antigenic determinant; the lorcaserin artificial antigen prepared further can be used for immunization to obtain an anti-lorcaserin artificial antibody with high affinity, high sensitivity and high specificity, the titer of the immune serum obtained by immunizing a New Zealand white rabbit is as high as 1:256000, and the immune serum can be used for rapid and accurate immunodetection and immunological analysis of lorcaserin.

The present disclosure relates, at least in part, to compositions comprising microgels and / or granular hydrogels with tailored surface biochemical properties that can serve, e.g., as artificial antigen-presenting cells (aAPCs) to mediate, e.g., T cell activation and expansion. Such microgels and / or granular hydrogels can be used to manufacture T cells for adoptive therapy in cancer treatment and tissue regeneration, and can be configured to serve, e.g., as a readily tunable and modular system to enable both rapid T cell expansion and control over T cell phenotype. Some aspects of the present disclosure provide methods and compositions for fabricating microgels and / or granular hydrogels, and modulating the surface properties of microgels and / or granular hydrogels by functionalizing, e.g., via layer- by-layer coating.

The invention relates to the technical field of small molecule chemical detection, in particular to a raclosibin artificial hapten, an artificial antigen and a preparation method and application of the raclosibin artificial hapten and the artificial antigen. The invention provides a raclosibin artificial hapten with a chemical structure as shown in a formula I. The artificial hapten maximally retains a characteristic structure of raclosibin, has an active group capable of being coupled with carrier protein, can be used as an antigenic determinant, and is high in sensitivity during immunoassay; the invention provides a raclosibin artificial antigen based on the raclosibin artificial hapten, and the raclosibin artificial antigen can be applied to colloidal gold immunochromatography detection of raclosibin and has relatively high sensitivity, detection gradient and relatively good specificity.

The invention discloses a chimeric antigen receptor T cell (CAR-T) of modular artificial antigen-natural antigen logic gating (MANAGE) and application of the chimeric antigen receptor T cell in treatment of solid tumors. At present, solid tumors still lack efficient targets capable of covering tumor cells of the same focus and crossing tumor species, and most solid tumor targets are also expressed on normal tissue cells. Therefore, the application of single-target CAR-T therapy in solid tumors is limited by tumor heterogeneity and non-tumor targeted toxicity (OTOT). According to the invention, proteins and antibodies of targeted tumor cells modified by the artificial antigen FITC are constructed, and CAR-T double-gated by the FITC and the natural tumor antigen is researched and developed. When the two compounds are combined for use, the toxicity of OTOT can be reduced, the range of applicable cells and tumor species can be expanded, the injection time of FITC can be regulated and controlled, the effect of CAR-T can be'switched ', cytokine syndromes can be reduced, and a new method is provided for solid tumor treatment.

The application discloses a kind of based on nano-silver-porous silicon simultaneously SERS detection variety of mycotoxin biochip sensor and its preparation method and application, the sensor includes SERS substrate and SERS label two parts compositions, SERS substrate is by porous silicon surface magnetron sputtering on silver nanoparticles and silver nanoparticles surface connection three kinds of mycotoxin artificial antigen composition, SERS label is connected with nile blue and corresponding mycotoxin antibody gold nanoparticles.The application can be combined by each mycotoxin and porous silicon silver surface mycotoxin artificial antigen mycotoxin antibody on SERS label, by detecting the Raman signal of NBA on SERS label surface, realize three kinds of mycotoxin simultaneous quantitative analysis, detection speed is fast, sensitivity is high, while various toxins in gualou, malt and kudzu root recovery rate is all greater than 75%, the coefficient of variation of same batch is less than 5.2%, and the coefficient of variation of different batches is less than 7.8%.

This invention discloses a hapten, an artificial antigen, and their applications for detecting paclobutrazol. The paclobutrazol hapten prepared by this invention retains the characteristic structure of paclobutrazol to the greatest extent possible. The paclobutrazol antibody prepared using the paclobutrazol hapten of this invention exhibits high potency and specificity, with a half-maximum inhibitory concentration (WMC) of 0.31 ng / mL and a linear range of 0.03–19.63 ng / mL. Furthermore, it shows no cross-reactivity with other structural and functional analogs such as uniconazole and hexaconazole. This invention simplifies the synthesis steps of the paclobutrazol hapten, and the method for preparing the paclobutrazol artificial antigen and its antibody is simple and low-cost. It also establishes a more efficient immunoassay method, capable of meeting the requirements for large-scale on-site sample testing and has broad application prospects.

The application discloses a kind of enrofloxacin rapid detection methods based on fluorescent covalent organic framework magnetic beads, belong to food safety detection field.The method uses the fluorescent covalent organic framework magnetic bead of coupling enrofloxacin monoclonal antibody, realizes detection in combination with immunochromatography test strip.Fluorescent covalent organic framework magnetic bead realizes target enrichment separation with inner core, shell fluorescent covalent organic framework material high-efficiency coupling antibody and exports fluorescence signal;Test strip is equipped with detection line (coated enrofloxacin artificial antigen) and quality control line (coated secondary antibody).By comparing the fluorescence intensity of detection line and quality control line, trace enrofloxacin in complex matrix can be qualitatively and quantitatively analyzed.The method is simple, rapid and sensitive, without large instrument, can be detected on site.

The application discloses a hapten of abamectin B1a and / or its structural analog, an artificial antigen and application thereof. The application connects a spacer to abamectin after hydrolysis to obtain a hapten of abamectin B1a and / or its structural analog, and then connects a carrier protein to prepare an artificial antigen, and further prepare a monoclonal antibody. A chemiluminescence immunoassay method is established according to specific recognition of the antibody and the antigen, and abamectin residues in a sample are detected. The method can be used for trace detection of abamectin residues, and can avoid a complicated pretreatment step. The detection limit is 0.03 ng / mL, the IC 50 (50% inhibitory concentration) is 0.1 ng / mL, and the linear range IC 20 ~IC 80 is 0.04-0.20 ng / mL.

This invention provides an allopurinol hapten, its preparation method, an artificial antigen, an antibody, and their applications. The structure of the allopurinol hapten is shown below. The allopurinol hapten provided by this invention has a spacer arm of suitable length, which allows the characteristic structure of the target molecule to be far away from the carrier protein, maximizing the exposure of the hapten to the immune system while minimizing recognition of the spacer arm, resulting in better antibody recognition of allopurinol. This invention uses this allopurinol hapten to prepare artificial antigens and antibodies, obtaining allopurinol monoclonal antibodies with high titer, strong specificity, and high affinity. The immunoassay method based on this allopurinol artificial antigen and antibody has high specificity and sensitivity, and no cross-reactivity with allopurinol analogs, enabling rapid qualitative and quantitative detection of allopurinol.

In various aspects and embodiments, this disclosure provides artificial antigen-presenting cells (aAPCs) suitable for parenteral administration for immunotherapy. In various embodiments, aAPCs effectively activate or inhibit target T cells, including CD8+ or CD4+ T cells, in vivo. The aAPCs according to this disclosure provide a storage-stable nanoparticle platform for immunotherapy. In various embodiments, the storage-stable nanoparticle platform controls characteristics such as particle size and particle chemistry, ligand design and ligand density, and aAPC aggregation tendency.

The invention discloses a method for detecting lactoferrin in milk based on a competition method test strip, which comprises the following steps: coupling heparin molecules with bovine serum albumin to obtain a heparin molecule-bovine serum albumin artificial antigen; modifying gold nanoparticles by using an artificial antigen to form a gold label I, and constructing a competition method test strip by matching with a gold label II modified by mouse IgG (Immunoglobulin G); a milk sample is subjected to centrifugal fat removal, pH adjustment and casein precipitation pretreatment and then dropwise added to the test strip, and the LF concentration is calculated through a T line gray value and a working curve. Detection can be completed within about 10-15 min, specificity is high (interference of impure protein is avoided), on-site rapid quantitative / semi-quantitative detection of LF in milk can be achieved, the antiviral activity of LF can be indirectly reflected through the binding strength of heparin and LF, the method is suitable for production quality inspection, dealer sampling inspection and consumer self-inspection, and the detection sensitivity reaches 1 microgram / mL.

The invention relates to a chlorantraniliprole hapten, a complete antigen and application of the chlorantraniliprole hapten and the complete antigen. When the chlorantraniliprole artificial antigen and the monoclonal antibody are used for ELISA detection, the specificity is high, the IC50 value is 0.058 [mu] g / L, the detection limit of chlorantraniliprole in a standard solution reaches 0.6 [mu] g / L, and the detection limit of a sample to be detected reaches 2 g / kg; the cross reaction rates with cyantraniliprole, tetrachlorantraniliprole and cyclaniliprole are respectively 20%, 12% and 12%, and the material specificity is greatly improved. By utilizing the chlorantraniliprole hapten, the complete antigen and the antibody, the purpose of rapidly and accurately detecting chlorantraniliprole in a sample can be achieved.

This application discloses an artificial antigen and an artificial antibody for detecting sennosides and their applications. The structural formula of the artificial antigen is shown in (I), the amino acid sequence of the heavy chain of the artificial antibody is shown in SEQ ID NO: 1, and the amino acid sequence of the light chain is shown in SEQ ID NO: 2. A kit for detecting sennosides is constructed using the artificial antigen and / or the artificial antibody, and an immunological method for detecting sennosides is established. When sennosides are detected using the kit or the immunological method shown, specific and accurate detection of sennosides can be achieved, with excellent detection sensitivity and fast detection time, which can be used for rapid clinical detection of actual samples.

The invention discloses an ochratoxin A hapten, an ochratoxin A artificial antigen, an ochratoxin A antibody and a preparation method and application thereof, and relates to the technical field of immunochemistry and biotoxin detection. The hapten is prepared from ochratoxin A and succinic anhydride through reaction, the artificial antigen is prepared from the hapten and isobutyl chloroformate, and the antibody is prepared from animals immunized by the artificial antigen. The antigen and the antibody can be applied to preparation of an ochratoxin A colloidal gold immunochromatography test strip, and can rapidly and sensitively detect ochratoxin A residues in animal-derived food.

The application discloses an antibody against ochratoxin A and application thereof. The application uses an artificial antigen obtained by coupling ochratoxin A and a carrier protein as an immunogen to prepare a monoclonal antibody against ochratoxin A. The monoclonal antibody can sensitively and specifically detect ochratoxin A, and the minimum detection limit (LOD) is 0.18 ng / mL. In addition, the half inhibition (IC 50 ) of the icELISA for ochratoxin A established by using the monoclonal antibody against ochratoxin A is 1.42 ng / mL, and the linear range is 0.34-5.40 ng / mL. That is, the application provides an antibody capable of detecting ochratoxin A and a method for detecting ochratoxin A, and has the advantages of simplicity, rapidness, strong specificity, wide linear range and high sensitivity.

This invention discloses a pyrimethanil hapten, an artificial antigen, and their preparation methods and applications. The structural formula of the pyrimethanil hapten is shown below. The arm introduced in this invention not only possesses an active group but also fully retains the benzene ring and dimethylpyrimidine ring structure of the target analyte, enhancing the recognition site. The pyrimethanil artificial antigen and monoclonal antibody of this invention exhibit high specificity for ELISA detection, with an IC50 rating of [missing information]. 50 The value is 1.97 μg / L, which is better than existing monoclonal antibodies. It can be used in colloidal gold immunochromatography to rapidly and conveniently detect pyrimethanil. The colloidal gold immunochromatographic test strip prepared in this invention has a detection sensitivity of 10 μg / L for pyrimethanil.

The present application belongs to the field of rapid detection, and provides a small molecule detection kit. The traditional detection of small molecules by immunochromatographic competition inhibition mode is converted into a double-antibody sandwich mode through a conversion column and a general mouse IgG immunochromatographic test strip. The reverse reading signal on the detection line is converted into a positive reading signal, so that the trace existence of small molecules can make the detection line of the test strip appear a signal, and compared with the traditional competitive immunochromatographic method, the present application has the characteristics of high detection sensitivity. The conversion column is filled and formed after the target small molecule or its artificial antigen is covalently coupled with agarose or cellulose gel; the mouse IgG immunochromatographic test strip is composed of five parts, i.e. filter paper, sample pad, combination and release pad (fixed with a probe having signal output capability, i.e. labeled anti-mouse IgG monoclonal antibody 1 colloidal gold or fluorescent microspherical nanomaterial), NC membrane (anti-mouse IgG monoclonal antibody 2 is fixed on the detection line, and Protein G is fixed on the control line), and water absorption paper.

The application provides a flumetover half antigen, artificial antigen and antibody and a preparation method of the flumetover half antigen, artificial antigen and antibody, and application of the flumetover half antigen, artificial antigen and antibody to an enzyme-linked immunoassay kit, the kit comprising: an enzyme-labeled plate coated with a coating agent, a flumetover standard solution, a flumetover antibody, an enzyme conjugate concentrate, an enzyme conjugate diluent, a substrate developing solution, a termination solution and a washing solution, wherein the coating agent is a flumetover conjugate antigen, the enzyme conjugate is an enzyme-labeled flumetover antibody, and the flumetover antibody is obtained by immunizing animals with an immunogen. The application further discloses a method for detecting flumetover by using the enzyme-linked immunoassay kit, which comprises the following steps: firstly, sample pretreatment, then, detection by using the kit, and finally, analysis of the detection result. The enzyme-linked immunoassay kit provided by the application can be used for detecting the content of flumetover in vegetable and fruit samples, and has the advantages of simple operation, low cost, high sensitivity, on-site monitoring and suitability for screening of a large number of samples.

This invention relates to the field of biochemical technology, and particularly to highly specific haptens of polymyxin B and colistin, artificial antigens, their preparation methods, and applications. The haptens have structures as shown in Formula I or Formula II. The applications include: (1) preparing specific antibodies against polymyxin B or colistin; (2) detecting specific antibodies against polymyxin B or colistin; and (3) preparing reagents for detecting specific antibodies against polymyxin B or colistin. This invention further provides artificial antigens obtained by conjugating the haptens with carrier proteins. Using the artificial antigens provided by this invention as immunogens to immunize experimental animals, highly specific and sensitive antiserum can be prepared, which can then be used to extract and prepare polyclonal antibodies. These methods can be used to establish highly specific, highly sensitive, simple, and rapid detection methods for polymyxin B and colistin, and have significant application value.

The invention relates to an azithromycin hapten, an azithromycin artificial antigen and application thereof. The azithromycin A is used as an initial raw material, an active arm is introduced from a secondary amine terminal, all hydroxyl and tertiary amine structures of an azithromycin drug are completely reserved, the prepared hapten has a relatively good space structure, the electron cloud density of the hapten is basically consistent with that of an original drug, and the immunogenicity of the antigen is improved; the prepared azithromycin artificial antigen and the monoclonal antibody are high in specificity when being used for ELISA (enzyme-linked immunosorbent assay) detection, and the IC50 value is 0.03 g / L; a further established colloidal gold immunochromatography test strip can rapidly and conveniently realize qualitative detection of azithromycin, the sensitivity of the azithromycin in a standard solution is 0.5 g / kg, and the detection sensitivity of the azithromycin in a sample can reach 0.5 g / kg.

Biodegradable particles for interacting with immune cells to generate an immunosuppressive effect are disclosed. The biodegradable particle comprises a polyester or polyester blend with at least one soluble protein or small molecule encapsulated within the particle and at least two types of protein attached to a surface of the particle or to a coating on the surface thereof, which can be used to induce targeting regulatory T cells (Tregs). The at least two types of protein attached to a surface of the particle or to a coating on the surface thereof include a “Signal 1” protein that binds to an immune cell and a “Signal 2” protein that acts as a co-stimulatory molecule to immune cells. The encapsulated protein can be an interleukin and / or a cytokine. Methods of their use for treating a disease or condition, including an autoimmune disease, are disclosed.

The present invention relates to artificial antigen presenting cell (aAPC) scaffolds to provide cells with specific functional stimulation to obtain phenotypic and functional properties ideal to mediate tumor regression or viral clearance. In particular, the scaffolds of the present invention comprise stabilized MHC class I molecules comprising a heavy chain comprising an alpha-1 domain and an alpha-2 domain connected by a disulfide bridge, wherein said MHC class I molecules are free of antigenic peptide. The scaffolds can be loaded with antigenic peptide on demand, providing an agile platform for effective expansion and functional stimulation of specific T cells in a peptide-MHC-directed fashion.

The invention relates to the technical field of biochemical engineering, in particular to fipronil hapten and a preparation method thereof, artificial antigen, monoclonal antibody and application. The hapten has a chemical structure as shown in a formula I or a formula II. The invention also relates to an artificial antigen obtained by connecting the hapten and a carrier protein. The application comprises the following steps: (1) preparing a specific antibody for resisting fipronil or main metabolites thereof; (2) detecting a specific antibody resisting fipronil or main metabolites thereof; and (3) preparing a reagent for detecting a specific antibody resisting fipronil or main metabolites thereof. The hapten provided by the invention can expose common epitopes of fipronil and metabolites thereof to the greatest extent, can be used for preparing broad-spectrum specific monoclonal antibodies with uniform cross reaction rate and high sensitivity, and can realize high-specificity and sensitivity detection of fipronil or main metabolites thereof.

The invention discloses a diflubenzuron hapten, a diflubenzuron artificial antigen and a preparation method and application thereof, and relates to a diflubenzuron hapten, a diflubenzuron artificial antigen, an immunochromatography test paper card and a preparation method and application thereof. The diflubenzuron hapten prepared by the invention completely retains three characteristic groups of m-difluorophenyl, chlorphenyl and acyl urea of diflubenzuron, and a designed coupling arm is directly connected with a benzene ring, so that a small molecular structure on the prepared diflubenzuron artificial antigen not only perfectly retains the original characteristics, but also has an excellent space structure, and the diflubenzuron hapten can be used for preparing a diflubenzuron artificial antigen. The immunogenicity of the antigen is improved; the artificial antigen and the monoclonal antibody prepared from the hapten are high in specificity when being used for ELISA (enzyme-linked immunosorbent assay) detection, and the IC50 value is 0.14 g / L; when being used in a colloidal gold immunochromatography technology, the sensitivity of the diflubenzuron in a standard solution is 0.5 g / kg, and the detection sensitivity of the diflubenzuron in a sample is 5g / kg.