12 results about "Artificial antigen" patented technology

In various aspects and embodiments, this disclosure provides artificial antigen-presenting cells (aAPCs) suitable for parenteral administration for immunotherapy. In various embodiments, aAPCs effectively activate or inhibit target T cells, including CD8+ or CD4+ T cells, in vivo. The aAPCs according to this disclosure provide a storage-stable nanoparticle platform for immunotherapy. In various embodiments, the storage-stable nanoparticle platform controls characteristics such as particle size and particle chemistry, ligand design and ligand density, and aAPC aggregation tendency.

This application discloses an artificial antigen and an artificial antibody for detecting sennosides and their applications. The structural formula of the artificial antigen is shown in (I), the amino acid sequence of the heavy chain of the artificial antibody is shown in SEQ ID NO: 1, and the amino acid sequence of the light chain is shown in SEQ ID NO: 2. A kit for detecting sennosides is constructed using the artificial antigen and / or the artificial antibody, and an immunological method for detecting sennosides is established. When sennosides are detected using the kit or the immunological method shown, specific and accurate detection of sennosides can be achieved, with excellent detection sensitivity and fast detection time, which can be used for rapid clinical detection of actual samples.

This invention discloses a pyrimethanil hapten, an artificial antigen, and their preparation methods and applications. The structural formula of the pyrimethanil hapten is shown below. The arm introduced in this invention not only possesses an active group but also fully retains the benzene ring and dimethylpyrimidine ring structure of the target analyte, enhancing the recognition site. The pyrimethanil artificial antigen and monoclonal antibody of this invention exhibit high specificity for ELISA detection, with an IC50 rating of [missing information]. 50 The value is 1.97 μg / L, which is better than existing monoclonal antibodies. It can be used in colloidal gold immunochromatography to rapidly and conveniently detect pyrimethanil. The colloidal gold immunochromatographic test strip prepared in this invention has a detection sensitivity of 10 μg / L for pyrimethanil.

This invention relates to the field of biochemical technology, and particularly to highly specific haptens of polymyxin B and colistin, artificial antigens, their preparation methods, and applications. The haptens have structures as shown in Formula I or Formula II. The applications include: (1) preparing specific antibodies against polymyxin B or colistin; (2) detecting specific antibodies against polymyxin B or colistin; and (3) preparing reagents for detecting specific antibodies against polymyxin B or colistin. This invention further provides artificial antigens obtained by conjugating the haptens with carrier proteins. Using the artificial antigens provided by this invention as immunogens to immunize experimental animals, highly specific and sensitive antiserum can be prepared, which can then be used to extract and prepare polyclonal antibodies. These methods can be used to establish highly specific, highly sensitive, simple, and rapid detection methods for polymyxin B and colistin, and have significant application value.

This invention discloses a hapten, an artificial antigen, and their preparation method and application for cycloalkanoic acid markers, relating to the field of artificial antigen technology. A hapten for a cycloalkanoic acid marker is a compound represented by Formula I in Figure 1; an artificial antigen for a cycloalkanoic acid marker is a complete antigen prepared by conjugating the hapten of the compound represented by Formula I with a carrier protein. The core of this invention lies in introducing a specific molecular arm onto the cyclopentylpropionic acid molecule to construct a structurally optimized hapten, which is then conjugated with a carrier protein to form an artificial antigen. It is particularly important to note that the prepared artificial antigen is essentially a polypeptide, capable of effectively stimulating the body's immune response and producing antibodies with high affinity and high specificity.

The application relates to a monoclonal antibody of alpha, beta and gamma amatoxin and a preparation method and application thereof. The monoclonal antibody of alpha amatoxin is prepared from an alpha amatoxin artificial antigen, the monoclonal antibody of beta amatoxin is prepared from a beta amatoxin artificial antigen, and the monoclonal antibody of gamma amatoxin is prepared from a gamma amatoxin artificial antigen; the alpha amatoxin artificial antigen is a conjugate of alpha amatoxin and keyhole limpet hemocyanin, the beta amatoxin artificial antigen is a conjugate of beta amatoxin and bovine serum albumin, and the gamma amatoxin artificial antigen is a conjugate of gamma amatoxin and human serum albumin. The three antibodies have a small cross-reaction rate, can simultaneously detect and distinguish alpha-AMA, beta-AMA and gamma-AMA in a sample, and have high accuracy.

The present application belongs to the technical field of polycyclic aromatic hydrocarbon artificial antigen synthesis, and relates to a novel complete antigen synthesis method for detecting polycyclic aromatic hydrocarbon (PAHs), benzanthracene. The present application successfully prepares a hapten through a Friedel-Crafts acylation reaction, and prepares a complete antigen by coupling the hapten esterized hapten with a carrier protein. The complete antigens have been identified. The present application provides a novel hapten screening, preparation and efficient artificial antigen preparation method for the immunological analysis method of benzanthracene.

This invention discloses benzoic acid hapten, antigen, antibody, and their preparation methods and applications, involving benzoic acid hapten, antigen, antibody, fluorescence immunochromatographic detection device, and its application in detecting benzoic acid residues. This invention prepares benzoic acid hapten and artificial antigen through a total synthesis method. This method preserves the carboxylic acid group in the benzoic acid structure, ensuring the integrity of the benzoic acid hapten, and further successfully prepares a specific antibody for detecting benzoic acid, with an IC50 value of [missing information]. 50 The effective value was 5.89 ng / mL, and the linear detection range was 1.56–22.27 ng / mL. Furthermore, the benzoic acid antibody prepared using the hapten and antigen of this invention showed cross-reactivity rates of less than 0.01% with common structural analogs, demonstrating high accuracy and effectively eliminating interference from other structural analog drugs. This provides a core reagent for establishing a rapid detection method for benzoic acid.

The application discloses a fentanyl artificial hapten, a fentanyl artificial antigen and a preparation method and application thereof. The fentanyl artificial hapten of the application retains the characteristic structure of fentanyl to the maximum extent, and has an active group which can be coupled with a carrier protein and can serve as an antigenic determinant; the fentanyl artificial antigen prepared further can be used for immunization to obtain an anti-fentanyl antibody with high affinity, high sensitivity and high specificity, and the titer of the immune serum obtained by immunizing a New Zealand white rabbit is as high as 1:85000. The antigen and antibody can be used for enzyme-linked immunoassay, immunochromatography and the like, have the characteristics of high sensitivity, high specificity, strong operability and the like, and can be used for screening and detection of fentanyl components in various complex samples.

This invention discloses a pyrifensulfuron hapten, an artificial antigen, an antibody, and their preparation methods and applications. The structure of the pyrifensulfuron hapten is shown in Formula (I). This invention obtains a hapten by introducing a carboxyl active group with a certain length of spacer arm onto the benzene ring of pyrifensulfuron, which can then be coupled with a carrier protein to obtain an artificial antigen for immunization. This pyrifensulfuron hapten retains all the characteristic groups of pyrifensulfuron, with minimal alteration to the original structural features of pyrifensulfuron, laying the foundation for subsequent stimulation of animal immune responses to produce antibodies with higher specificity and sensitivity. The artificial antigen and antibody are then prepared using the above hapten. The resulting pyrifensulfuron monoclonal antibody, based on an indirect competitive ELISA using the pyrifensulfuron monoclonal antibody, shows a limit of detection of 2.30 ng / mL for pyrifensulfuron, with an IC50 value of [missing value]. 50 The effective concentration was 17.65 ng / mL, with a linear range of 4.87–63.94 ng / mL, indicating high detection sensitivity and a wide linear range.

The invention relates to a method for analyzing a biological sample. This includes providing a biological sample containing at least one target antigen (11, 12), providing at least one antibody (21, 22) immobilized on a support (20) to which an artificial antigen (31, 32) is bound, the artificial antigen carrying a nucleic acid (41, 42), wherein the target antigen (11, 12) has a higher affinity for the antibody (21, 22) than the artificial antigen (31, 32), bringing the antibody (21, 22) into contact (51) with the target antigen (11, 12), wherein the target antigen (11, 12) displaces the artificial antigen (31, 32) from the antibody (21, 22) (52), amplifying (55) the nucleic acid (41, 42) of the released artificial antigen (31, 32), and detecting the target antigen (11, 12) by detecting the amplified nucleic acid (41, 42).Furthermore, the invention relates to a device for analyzing a biological sample, which is configured to carry out the steps of the method.