148 results about "Artificial antigen" patented technology

The invention belongs to the technical field of immunodetection, and discloses a hapten and an artificial antigen for sulfonamide drug detection, a construction method and an application thereof, the structure of the hapten and the artificial antigen is shown as a formula (1): R1 =-COOH, R2 = R3 = H; r2 =-COOH, R1 = R3 = H; r3 is-COOH, and R1 is equal to R2 and H is equal to H. The invention discloses a novel sulfonamide immune hapten and a construction method of an artificial antigen of the novel sulfonamide immune hapten for the first time, and successfully obtains a specific antibody with high titer, high sensitivity and broad-spectrum recognition capability on the basis of immune animals of the artificial antigen.

The invention provides a safrole hapten and application of the safrole hapten in detection of safrole through immunoassay. The structure of the safrole hapten is shown in the specification. The two safrole haptens provided by the invention have spacer arms with proper lengths, and after the safrole haptens are coupled with carrier protein, the haptens can be fully exposed, and recognition of the spacer arms can be avoided as much as possible. The safrole hapten is used for preparing an artificial antigen and an antibody, and the obtained safrole monoclonal antibody is high in titer, strong in specificity and high in affinity. An immunoassay method constructed on the basis of the safrole artificial antigen and antibody has high specificity and sensitivity, the lowest detection limit LOD is 0.09 ng / mL, the half inhibitory concentration IC50 is 2.05 ng / mL, the quantitative detection range is 0.28-14.89 ng / mL, no cross reaction exists on safrole analogues, and rapid qualitative and quantitative detection can be conducted on safrole in a sample.

The invention discloses a hapten and an artificial antigen for detecting chlorfenapyr and application of the hapten and the artificial antigen. A time-resolved fluorescent test strip for detecting chlorfenapyr is prepared from the artificial antigen. The kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like, the quantitative detection limit of chlorfenapyr is as low as 3.48 ng / mL, the addition recovery rate is 99.6%-110.4%, the coefficient of variation is 2.1%-10.9%, and the kit is very suitable for rapid qualitative or quantitative detection of chlorfenapyr in plant-derived food by basic field personnel.

The invention discloses a phthalimidic acid hapten, an artificial antigen, an antibody, and a preparation method and application of the phthalimidic acid hapten and the artificial antigen. The structure of the phthalimide acid hapten is shown as a formula (I), and the phthalimide acid hapten is obtained by introducing a carboxyl active group with a spacer arm with a certain length to carboxyl of phthalimide acid, so that the phthalimide acid hapten can be coupled with carrier protein to obtain an artificial antigen for immunization; the phthalimide hapten retains all characteristic groups of the phthalimide, the original structural characteristics of the phthalimide are changed to the minimum extent, and a foundation is laid for generating an antibody with higher specificity and higher sensitivity for subsequently stimulating animal immune response. An artificial antigen and a phthalimide acid monoclonal antibody are prepared through the hapten, indirect competitive ELISA based on the monoclonal antibody has the lowest detection limit of phthalimide acid of 2.44 ng / mL, IC50 of 17.29 ng / mL and the linear range of 5.02-59.51 ng / mL, the detection sensitivity is high, and the linear range is wide.

The invention provides an analgin hapten, a preparation method thereof, an artificial antigen, an antibody and application. The structures of the analgin haptens are as shown in a formula (I) or a formula (II), the two analgin haptens provided by the invention have spacer arms with proper lengths, and after the two analgin haptens are coupled with protein, the haptens can be fully exposed to an immune system, the spacer arms can be prevented from being recognized as much as possible, and a better antibody is generated to recognize analgin. The analgin hapten is used for preparing artificial antigen and antibody, the obtained analgin monoclonal antibody has high titer and strong specificity, an immunoassay method constructed based on the analgin monoclonal antibody has high specificity and sensitivity, analgin in a sample can be rapidly, qualitatively and quantitatively detected, the operation is simple and convenient, and the detection result is accurate and reliable.

The application discloses a kind of areca alkaloid artificial hapten, artificial antigen and its preparation method and application, with norarecoline hydrochloride as the starting material for synthesizing artificial hapten.This areca alkaloid artificial hapten retains the characteristic structure of areca alkaloid to the greatest extent, and has active groups that can couple with carrier proteins, which can be used as antigenic determinants;Further, the areca alkaloid artificial antigen obtained can be used to immunize to obtain an anti-areca alkaloid artificial antibody with high affinity, high sensitivity and strong specificity.The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:70000, and can be used for rapid and accurate immunodetection and immunoassay of areca alkaloid.

The invention discloses a human intragastric factor protein polyclonal antibody and a preparation method thereof. The preparation method comprises the steps of artificial antigen design, animal immune optimization and antibody purification and verification. Accurate epitope design is achieved, structural interference of natural GIF is avoided through artificial polypeptide, and the cross reaction rate is lt; 5%; an efficient immune strategy is adopted, so that the immune period is shortened to 8-10 weeks, and the titer is increased by 2-3 times; low-cost production is realized, natural protein is replaced by the synthesized polypeptide, and the cost is reduced by 60%; the specificity is high, the cross reaction is reduced due to the design of the artificial antigen, and verification data supports specific recognition of natural GIF; large-scale production, polypeptide synthesis and a double-stage purification process are adopted, so that the consistency between batches is high (CV is less than or equal to 8%); the application range is wide, and diagnosis, pathological research and treatment monitoring scenes are covered.

The present invention provides an ELISA kit for detecting triclosan residues based on nanobody and its application. The kit includes a box body, a detachable enzyme-labeled plate disposed in the box body, and reagents disposed in the box body; wherein, each well of the enzyme-labeled plate is coated with a triclosan artificial antigen, and the reagents include the nanobody shown in SEQ ID NO:1, as well as a triclosan standard solution, an enzyme-labeled secondary antibody, a PBS buffer solution, a PBST washing solution, a chromogenic solution, a reaction termination solution, etc. The kit of the present invention can accurately and sensitively detect triclosan residues in environments such as water, soil, and vegetables. The sample pretreatment process is simple and time-consuming less, and a large number of samples can be detected simultaneously. The sample detection cost is much lower than the traditional instrument detection method.

The invention discloses design and synthesis of a hapten of cyromazine, development of a cyromazine artificial antigen and a cyromazine antibody, and application of the cyromazine artificial antigen and the cyromazine antibody in the field of food safety rapid detection. The aminocarboxylic acid modified cyromazine hapten product is prepared through the steps of cyromazine hapten design, connecting arm introduction, synthesis, purification and the like. The cyromazine hapten with the structure is coupled with keyhole limpet hemocyanin to form an artificial immune antigen with immunogenicity, and is coupled with chicken ovalbumin to form an artificial competitive antigen. Tests show that the artificial immune antigen has high immunocompetence and can stimulate an animal immune system to generate an anti-cyromazine antibody, and the titer of an immune Blab / c mouse serum antibody is 16000. The IC50 of an enzyme-linked immunosorbent assay (ELISA) detection method established by the cyromazine artificial competitive antigen and the anti-cyromazine antibody is 5.3 [mu] g / L, and the detection sensitivity of an established gold-labeled immunochromatography detection method reaches 50 [mu] g / L.

The present invention discloses amitraz hapten, antigen, antibody, detection device and preparation and application thereof, and relates to amitraz hapten, antigen, antibody, colloidal gold detection device and preparation and application thereof for detecting amitraz. The present invention provides a method for preparing artificial antigen by synthesizing amitraz hapten and coupling antigen with carrier protein, as well as antibodies specific to amitraz produced by the body of experimental animals after immunization, and the IC 50 The value was 5.22 ng / mL, and the linear detection range for amitraz was 1.82-14.96 ng / mL, which meets the limit for amitraz in the national standard GB 2763-2021, "National Food Safety Standard Maximum Limits of Pesticide Residues in Food." Therefore, the present invention can be applied to the rapid detection of amitraz residues in agricultural products.

The present invention relates to artificial antigen presenting cell (aAPC) scaffolds to provide cells with specific functional stimulation to obtain phenotypic and functional properties ideal to mediate tumor regression or viral clearance. In particular, the scaffolds of the present invention comprise stabilized MHC class I molecules free of antigenic peptide. The scaffolds can be loaded with antigenic peptide on demand, providing an agile platform for effective expansion and functional stimulation of specific T cells in a peptide-MHC-directed fashion.

The present invention belongs to the field of biochemical technology, and specifically relates to an artificial hapten and an artificial antigen for benzhexyphenidyl, as well as a preparation method and application thereof. The molecular structural formula of the artificial hapten for benzhexyphenidyl is shown in Formula (I), and the molecular structural formula of the artificial antigen for benzhexyphenidyl is shown in Formula (II). The artificial hapten for benzhexyphenidyl can be used in the field of preparing anti-benzhexyphenidyl antibodies. The artificial hapten for benzhexyphenidyl of the present invention retains the characteristic structure of benzhexyphenidyl to the greatest extent, and has an active group that can be coupled with a carrier protein, and can serve as an antigenic determinant. The artificial hapten for benzhexyphenidyl obtained by further preparation can be used to immunize and obtain anti-benzhexyphenidyl antibodies with high affinity, high sensitivity, and strong specificity. The titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:256,000, and can be used for rapid and accurate immunodetection and immunoanalysis of benzhexyphenidyl.

The present application belongs to the technical field of bio-chemical industry, and particularly relates to a lorcaserin artificial hapten, a lorcaserin artificial antigen, and a preparation method and application thereof. The lorcaserin hydrochloride is used as a starting material for synthesizing the artificial hapten, the lorcaserin artificial hapten of the present application retains the characteristic structure of lorcaserin to the greatest extent, and has an active group which can be coupled with a carrier protein, and can be used as an antigenic determinant; the lorcaserin artificial antigen prepared further can be used for immunization to obtain an anti-lorcaserin artificial antibody with high affinity, high sensitivity and high specificity, the titer of the immune serum obtained by immunizing a New Zealand white rabbit is as high as 1:256000, and the immune serum can be used for rapid and accurate immunodetection and immunological analysis of lorcaserin.

The present disclosure relates, at least in part, to compositions comprising microgels and / or granular hydrogels with tailored surface biochemical properties that can serve, e.g., as artificial antigen-presenting cells (aAPCs) to mediate, e.g., T cell activation and expansion. Such microgels and / or granular hydrogels can be used to manufacture T cells for adoptive therapy in cancer treatment and tissue regeneration, and can be configured to serve, e.g., as a readily tunable and modular system to enable both rapid T cell expansion and control over T cell phenotype. Some aspects of the present disclosure provide methods and compositions for fabricating microgels and / or granular hydrogels, and modulating the surface properties of microgels and / or granular hydrogels by functionalizing, e.g., via layer- by-layer coating.

The invention relates to the technical field of small molecule chemical detection, in particular to a raclosibin artificial hapten, an artificial antigen and a preparation method and application of the raclosibin artificial hapten and the artificial antigen. The invention provides a raclosibin artificial hapten with a chemical structure as shown in a formula I. The artificial hapten maximally retains a characteristic structure of raclosibin, has an active group capable of being coupled with carrier protein, can be used as an antigenic determinant, and is high in sensitivity during immunoassay; the invention provides a raclosibin artificial antigen based on the raclosibin artificial hapten, and the raclosibin artificial antigen can be applied to colloidal gold immunochromatography detection of raclosibin and has relatively high sensitivity, detection gradient and relatively good specificity.

The invention discloses a chimeric antigen receptor T cell (CAR-T) of modular artificial antigen-natural antigen logic gating (MANAGE) and application of the chimeric antigen receptor T cell in treatment of solid tumors. At present, solid tumors still lack efficient targets capable of covering tumor cells of the same focus and crossing tumor species, and most solid tumor targets are also expressed on normal tissue cells. Therefore, the application of single-target CAR-T therapy in solid tumors is limited by tumor heterogeneity and non-tumor targeted toxicity (OTOT). According to the invention, proteins and antibodies of targeted tumor cells modified by the artificial antigen FITC are constructed, and CAR-T double-gated by the FITC and the natural tumor antigen is researched and developed. When the two compounds are combined for use, the toxicity of OTOT can be reduced, the range of applicable cells and tumor species can be expanded, the injection time of FITC can be regulated and controlled, the effect of CAR-T can be'switched ', cytokine syndromes can be reduced, and a new method is provided for solid tumor treatment.

The application discloses a kind of based on nano-silver-porous silicon simultaneously SERS detection variety of mycotoxin biochip sensor and its preparation method and application, the sensor includes SERS substrate and SERS label two parts compositions, SERS substrate is by porous silicon surface magnetron sputtering on silver nanoparticles and silver nanoparticles surface connection three kinds of mycotoxin artificial antigen composition, SERS label is connected with nile blue and corresponding mycotoxin antibody gold nanoparticles.The application can be combined by each mycotoxin and porous silicon silver surface mycotoxin artificial antigen mycotoxin antibody on SERS label, by detecting the Raman signal of NBA on SERS label surface, realize three kinds of mycotoxin simultaneous quantitative analysis, detection speed is fast, sensitivity is high, while various toxins in gualou, malt and kudzu root recovery rate is all greater than 75%, the coefficient of variation of same batch is less than 5.2%, and the coefficient of variation of different batches is less than 7.8%.

The invention belongs to the technical field of polycyclic aromatic hydrocarbon artificial antigen synthesis, and relates to a novel synthetic method of a complete antigen for detecting polycyclic aromatic hydrocarbon (PAHs)-# imgabs0 #. The hapten is successfully prepared through Friedel-Crafts acylation reaction, and the hapten is esterified and then coupled with carrier protein to prepare the complete antigen. These complete antigens have been identified. The invention provides a novel hapten screening and preparation method and an efficient artificial antigen preparation method for an immunoassay method of # imgabs1 #.

The invention provides a medetomidine artificial hapten, an artificial antigen as well as a preparation method and application of the medetomidine artificial hapten and the artificial antigen. Specifically, the invention provides a medetomidine artificial hapten of formula (X), the artificial hapten maximally retains the characteristic structure of medetomidine, has an active group capable of being coupled with carrier protein, can be used as an antigenic determinant, and has high sensitivity during immunoassay; furthermore, the invention provides a medetomidine artificial antigen based on the medetomidine artificial hapten, and the medetomidine artificial antigen can be applied to colloidal gold immunochromatography detection of medetomidine and has relatively high sensitivity, detection gradient and relatively good specificity.

This invention discloses a hapten, an artificial antigen, and their applications for detecting paclobutrazol. The paclobutrazol hapten prepared by this invention retains the characteristic structure of paclobutrazol to the greatest extent possible. The paclobutrazol antibody prepared using the paclobutrazol hapten of this invention exhibits high potency and specificity, with a half-maximum inhibitory concentration (WMC) of 0.31 ng / mL and a linear range of 0.03–19.63 ng / mL. Furthermore, it shows no cross-reactivity with other structural and functional analogs such as uniconazole and hexaconazole. This invention simplifies the synthesis steps of the paclobutrazol hapten, and the method for preparing the paclobutrazol artificial antigen and its antibody is simple and low-cost. It also establishes a more efficient immunoassay method, capable of meeting the requirements for large-scale on-site sample testing and has broad application prospects.

The application discloses a kind of enrofloxacin rapid detection methods based on fluorescent covalent organic framework magnetic beads, belong to food safety detection field.The method uses the fluorescent covalent organic framework magnetic bead of coupling enrofloxacin monoclonal antibody, realizes detection in combination with immunochromatography test strip.Fluorescent covalent organic framework magnetic bead realizes target enrichment separation with inner core, shell fluorescent covalent organic framework material high-efficiency coupling antibody and exports fluorescence signal;Test strip is equipped with detection line (coated enrofloxacin artificial antigen) and quality control line (coated secondary antibody).By comparing the fluorescence intensity of detection line and quality control line, trace enrofloxacin in complex matrix can be qualitatively and quantitatively analyzed.The method is simple, rapid and sensitive, without large instrument, can be detected on site.

The present invention discloses a diafenthiuron hapten, an artificial antigen, preparation methods, antibodies, and applications thereof. The structural formula of the diafenthiuron hapten is #imgabs0#. The hapten, artificial antigen, antibodies to immunized animal products, and the test kit prepared in this invention exhibit high sensitivity and specificity, are simple to operate, have a short detection time, are not limited by specialized personnel, are inexpensive, and are suitable for grassroots deployment. This technology fills a gap in domestic rapid detection of diafenthiuron. The application of this technology can significantly accelerate detection and has significant social benefits in controlling diafenthiuron pesticide poisoning in edible agricultural products, reducing pesticide over-limits, improving the qualified rate of commercially available vegetables, and ensuring food safety.

The application discloses a hapten of abamectin B1a and / or its structural analog, an artificial antigen and application thereof. The application connects a spacer to abamectin after hydrolysis to obtain a hapten of abamectin B1a and / or its structural analog, and then connects a carrier protein to prepare an artificial antigen, and further prepare a monoclonal antibody. A chemiluminescence immunoassay method is established according to specific recognition of the antibody and the antigen, and abamectin residues in a sample are detected. The method can be used for trace detection of abamectin residues, and can avoid a complicated pretreatment step. The detection limit is 0.03 ng / mL, the IC 50 (50% inhibitory concentration) is 0.1 ng / mL, and the linear range IC 20 ~IC 80 is 0.04-0.20 ng / mL.

This invention provides an allopurinol hapten, its preparation method, an artificial antigen, an antibody, and their applications. The structure of the allopurinol hapten is shown below. The allopurinol hapten provided by this invention has a spacer arm of suitable length, which allows the characteristic structure of the target molecule to be far away from the carrier protein, maximizing the exposure of the hapten to the immune system while minimizing recognition of the spacer arm, resulting in better antibody recognition of allopurinol. This invention uses this allopurinol hapten to prepare artificial antigens and antibodies, obtaining allopurinol monoclonal antibodies with high titer, strong specificity, and high affinity. The immunoassay method based on this allopurinol artificial antigen and antibody has high specificity and sensitivity, and no cross-reactivity with allopurinol analogs, enabling rapid qualitative and quantitative detection of allopurinol.

In various aspects and embodiments, this disclosure provides artificial antigen-presenting cells (aAPCs) suitable for parenteral administration for immunotherapy. In various embodiments, aAPCs effectively activate or inhibit target T cells, including CD8+ or CD4+ T cells, in vivo. The aAPCs according to this disclosure provide a storage-stable nanoparticle platform for immunotherapy. In various embodiments, the storage-stable nanoparticle platform controls characteristics such as particle size and particle chemistry, ligand design and ligand density, and aAPC aggregation tendency.

The invention discloses a method for detecting lactoferrin in milk based on a competition method test strip, which comprises the following steps: coupling heparin molecules with bovine serum albumin to obtain a heparin molecule-bovine serum albumin artificial antigen; modifying gold nanoparticles by using an artificial antigen to form a gold label I, and constructing a competition method test strip by matching with a gold label II modified by mouse IgG (Immunoglobulin G); a milk sample is subjected to centrifugal fat removal, pH adjustment and casein precipitation pretreatment and then dropwise added to the test strip, and the LF concentration is calculated through a T line gray value and a working curve. Detection can be completed within about 10-15 min, specificity is high (interference of impure protein is avoided), on-site rapid quantitative / semi-quantitative detection of LF in milk can be achieved, the antiviral activity of LF can be indirectly reflected through the binding strength of heparin and LF, the method is suitable for production quality inspection, dealer sampling inspection and consumer self-inspection, and the detection sensitivity reaches 1 microgram / mL.

The invention relates to a chlorantraniliprole hapten, a complete antigen and application of the chlorantraniliprole hapten and the complete antigen. When the chlorantraniliprole artificial antigen and the monoclonal antibody are used for ELISA detection, the specificity is high, the IC50 value is 0.058 [mu] g / L, the detection limit of chlorantraniliprole in a standard solution reaches 0.6 [mu] g / L, and the detection limit of a sample to be detected reaches 2 g / kg; the cross reaction rates with cyantraniliprole, tetrachlorantraniliprole and cyclaniliprole are respectively 20%, 12% and 12%, and the material specificity is greatly improved. By utilizing the chlorantraniliprole hapten, the complete antigen and the antibody, the purpose of rapidly and accurately detecting chlorantraniliprole in a sample can be achieved.

The invention discloses an antibody for resisting ochratoxin A and application of the antibody. According to the invention, an artificial antigen obtained by coupling the ochratoxin A and a carrier protein is used as an immunogen to prepare the monoclonal antibody against the ochratoxin A. The ochratoxin A can be sensitively and specifically detected by using the monoclonal antibody, and the lowest limit of detection (LOD) of the monoclonal antibody is 0.18 ng / mL. In addition, the half inhibition (IC50) of icELISA (Enzyme-Linked Immunosorbent Assay), which is established by utilizing the monoclonal antibody for resisting the ochratoxin A, on the ochratoxin A is 1.42 ng / mL, and the linear range is 0.34 ng / mL to 5.40 ng / mL. The invention provides the antibody capable of being used for detecting the ochratoxin A and the method for detecting the ochratoxin A, and the antibody and the method have the advantages of being simple, convenient, rapid, high in specificity, wide in linear range and high in sensitivity.

This application discloses an artificial antigen and an artificial antibody for detecting sennosides and their applications. The structural formula of the artificial antigen is shown in (I), the amino acid sequence of the heavy chain of the artificial antibody is shown in SEQ ID NO: 1, and the amino acid sequence of the light chain is shown in SEQ ID NO: 2. A kit for detecting sennosides is constructed using the artificial antigen and / or the artificial antibody, and an immunological method for detecting sennosides is established. When sennosides are detected using the kit or the immunological method shown, specific and accurate detection of sennosides can be achieved, with excellent detection sensitivity and fast detection time, which can be used for rapid clinical detection of actual samples.

The present invention provides a methyl parathion hapten, a sibutramine hapten, a methyl parathion-sibutramine artificial antigen and antibody, and a detection kit, relating to the technical field of food safety detection. The polyclonal antibody obtained by immunization with the artificial antigen not only has high sensitivity but also can simultaneously identify both methyl parathion and sibutramine, thus solving the technical problem of the lack of a highly sensitive and rapid method for the simultaneous detection of methyl parathion and sibutramine in the prior art. The half inhibitory concentration (IC50) of the antibody for methyl parathion is 100%. 50 ) was 4.59 ng / mL, the quantitative detection range was 2.89 ng / mL-7.28 ng / mL, and the detection limit was 2.21 ng / mL; the half-inhibitory concentration (IC 50 ) was 59.76 ng / mL, the quantitative detection range was 37.65 ng / mL-94.87 ng / mL, and the detection limit was 28.73 ng / mL.