267 results about "Microcystin" patented technology

The invention discloses a BiOI composite material and a preparation method and an application of the BiOI composite material. The BiOI composite material is formed by doping noble metal materials, AgI or multi-walled carbon nanotubes in BiOI, wherein the noble metal materials comprises Pt and Ag. Catalysts refer to Pt/BiOI, Ag/BiOI, multi-walled carbon nanotubes (MWCNTs)/BiOI and AgI/BiOI Catalysts which are obtained by doping noble metal Pt, Ag or multi-walled carbon nanotubes and AgI on the basis of BiOI. The BiOI composite material is simple in preparation process, good in stability and narrow in energy band, can efficiently utilize solar energy and can be excited under visible light to generate substances with strong oxidizing property, such as hydroxyl free radical which can effectively remove microorganism, difficultly-biodegradable organic substances and microcystin from water.

The invention discloses a microcystlin monoclonal antibody and the preparation method and the application thereof. The microcystlin monoclonal antibody is a microcystlin-LR monoclonal antibody, and the preparation method thereof is that: 1) an amino group is introduced to a seventh amino acid residue N-methyl dehydroalanine on microcystlin-LR, thus obtaining amino modified microcystlin-LR polypeptide; the amino modified microcystlin-LR polypeptide is coupled with carrier protein to obtain complete A antigen; 2) the immunity mouse of complete A antigen obtained in step 1) to get spleen cell of immunity mouse, which then is fused with myeloma cell of mouse to screen out positive hybrid cancer cell strain; the positive hybrid cancer cell strain is cultured or is injected in sygeneous mouse abdominal cavity to induce ascites to get the microcystlin-LR monoclonal antibody. The monoclonal antibody can be used for examining microcystlin-LR and immunity affinity purification thereof.

The invention belongs to technical field of environmental-friendly water treatment and relates to an MC-LR (microcystin-LR) degrading strain screened from bottom sediments of streamsides with high blue-green algae pollution in the Taihu Lake and a method for degrading microcystins by the same. Currently, the strain is preserved in the China general microbiological culture collection center (CGMCC) and has a preservation number of CGMCC NO.4498. By identification, the strain is bacillus (bacillussp.). The method for degrading the microcystins by the MC-LR degrading strain comprises the following steps of: inoculating the MC-LR degrading strain into an inorganic salt culture medium which uses the MC-LR as the only carbon source and nitrogen source and has a pH value of 5 to 9 according to an inoculation rate of 2 to 10 mass percent; and cultivating the MC-LR degrading strain for 48 hours in a shaking table at a temperature of 30 DEG C under a rotating speed of 100 to 180 r/min so as to degrade the MC-LR. The stain is screened from a water body of the Taihu Lake, is suitable for the natural environment of the Taihu watershed and has wide application prospect for removing the MC-LR in the water body of the Taihu watershed by a biological method.

The invention discloses a method for detecting microcystin in water. The method comprises the following steps of: (1) preparing magnetic particles connected with monoclonal antibodies of microcystin; (2) preparing a Raman probe connected with microcystin molecules by Raman enhancement of silver nano particles on micromolecules; (3) drawing a standard detection curve of the microcystin under the determined optimum reaction condition; and (4) determining the concentration of a sample to be determined by comparing detected peak intensity of a specified peak of a Raman spectrum of the sample and the standard curve. In the detection process, laser Raman enhancement and competition immunity are combined, and magnetic separation is applied; and compared with the conventional detection method, the method for detecting the microcystin in water greatly shortens detection time. The method for detecting the microcystin in water can also be used for detecting other kinds of microcystin molecules and small molecules, and a quick and convenient detection way is provided with pollutant in water.

The invention belongs to the field of photocatalytic environmental protection, and particularly relates to a preparation method and application of a g-C3N4/BiOI composite visible light catalytic material for carrying out photocatalytic degradation on microcystin. Photocatalytic degradation on organic pollutants has been generally recognized as one of green environment purification technologies with the widest prospect. Compared with a conventional treatment process, a technology for carrying out photocatalytic degradation on the microcystin, which is adopted by the invention, has the advantages of no secondary pollution, complete removal on pollutants, safety, stability, low cost and the like, has an obvious degradation effect on toxic organic pollutants which take microcystin (MC-LR) as the representative, and has no related research results reported publicly. According to the invention, by adopting a solid-phase calcination method, a novel narrow band-gap semiconductor BiOI and nonmetal organic semiconductor graphite-phase carbon nitride (g-C3N4) are compounded to prepare the g-C3N4/BiOI composite visible light catalytic material; the composite material can effectively expand a spectral response range, promote separation and transfer of semiconductor photo-induced electron-hole pairs, reduce the compound probability of the semiconductor photo-induced electron-hole pairs and improve photocatalytic efficiency. The preparation method disclosed by the invention is simple in preparation process, mild in condition, high in stability and low in cost, and can efficiently utilize solar energy; the prepared composite photocatalytic material can be applied to the field of treatment on eutrophic water, and has high practical value and wide application prospect.

The ecological water plankton regulating agent consists of gypsum powder 30-40 wt%, attapulgite powder 20-40 wt% and sodium humate 30-40 wt%. The method of utilizing the ecological water plankton regulating agent in controlling excessive propagation of blue-green algae in water is to dilute the ecological water plankton regulating agent with 10-20 times water to obtain suspension and spreading the suspension to the water, with the applied amount of the ecological water plankton regulating agent being 3-10 g/cu m. The said regulating agent and method can control the excessive propagation of blue-green algae, ensure the algae diversity, raise the transparency of water and lower the content of microcystin in water.

The invention discloses a construction method and an application of an immobilized biological bacterium agent for a micro-polluted water source and belongs to the field of ecological remediation of water environments. The method is a treating process for purifying a water body through construction of the biological bacterium agent, and ammonia nitrogen, iron, manganese, microcystis, algal toxins and the like in the water body can be removed effectively. The method comprises steps as follows: (1) rescreening and compounding of multifunctional oligotrophic ammonia nitrogen bacteria; (2) screening of efficient algicidal bacteria; (3) fermentation and construction of the bacterium agent; (4) bacterium agent immobilization and biological enhancement; (5) research on dosing methods of the bacterium agent, and treatment and biological enhancement of the micro-polluted drinking water source. The biological bacterium agent is constructed with an immobilization technology, after a system runs stably, the removal rate of the ammonia nitrogen is higher than 80%, removal rates of the microcystis and the algal toxins are higher than 40%, the removal rate of the iron in water is 50%, and the removal rate of manganese is 51%. The method can be combined with actual projects and is wider in application prospect.

The invention discloses a ZnTCPP@MOF-based electrochemical immunoassay method for microcystic toxins. The method comprises the following steps: modifying an electrode by a porphyrin-functionalized metal-organic framework ZnTCPP@MOF; increasing the stability of the ZnTCPP@MOF on the electrode by utilizing tetra-n-octylammonium bromide; and dripping microcystic toxin MC-LR antigen and monoclonal antibody, and assembling to form an electrochemical immunosensor. The electrochemical immunosensor prepared by the method disclosed by the invention is capable of realizing high-sensitivity and high-specificity detection of the microcystic toxin MC-LR, the generated electrochemical luminescence signal is stable, the lower limit of detection on the microcystic toxin MC-LR is low, and the method has excellent application prospects in the field of detection of biological and environmental microcystic toxins.

The invention provides a process for utilizing three abandoned proteins and thoroughly-decomposed cow dung to produce a growth-promoting bio-organic fertilizer and a product thereof and belongs to agricultural intensive production technologies. A used formula comprises, by dry weight, 75% of thoroughly-decomposed cow dung ordinary manure, 5% of alga ooze, 10% of abandoned feather puffing powder and 10% of rapeseed meal. A used strain is a growth-promoting bacillus amyloliquefaciens SQR-9. The process comprises mixing the raw materials according to the optimum formula, adding 5% of growth-promoting bio-control bacteria by volume mass, performing strip stack type fermentation, performing overturning once each day, and fermenting for 6-7d. The number of beneficial viable bacteria in the produced fertilizer is lager than 2*10<8>CFU/g by dry weight (similarly hereinafter), the content of total nitrogen is 2-3%, the content of total NPK nutrient is 6-8%, the content of organic matter is larger than 45%, and the content of microcystin LR and the content of microcystin RR are both lower than 4ug/kg. The growth-promoting bio-organic fertilizer is rich in beneficial amino acid, low in production cost and high in growth-promoting effect and can be applied to food crops and vegetables and the like.

The invention relates to a method for the biodegradation of microcystins by using microorganisms, which belongs to the field of biotechnology. The method comprises the following steps: screening sphingosine monads USTB-05 of microorganism strains; using a cultured high-cell-density bacterial solution of the sphingosine monads USTB-05 or an enzyme extracted from cells as a biocatalyst; and removingMCs in a water body in an adding mode, or removing the MCs by applying the degradation in a sewage biological treatment process in the presence of the MCs. The method has the characteristics of safety, economy and high efficiency, and has important application values in the aspect of removing MCs pollutions generated by blue alga blooms through degradation.

The invention belongs to the technical field of toxin detection and discloses an SPR sensor for detecting microcystic toxin based on a nucleic acid aptamer signal amplification strategy and a preparation method and application thereof. The SPR sensor comprises a glass chip, a microcystic toxin aptamer, a DNA probe 1, a DNA probe 2 and a precious metal nano material, wherein microcystic toxin detection can be performed by determining SPR signal change. The preparation of the SPR sensor is simple and facilitates establishment of a portable detecting instrument for field monitoring, and the cost is far lower than that of a large analysis and detection instrument.

The invention discloses a method for separating and purifying microcystin by utilizing series-connected solid phase extraction columns. The method comprises the following steps of: A, preparing an algae powder material, namely harvesting wild bloom-forming cyanobacteria or microcystis aeruginosa cultured indoors, cooling and drying to prepare dried algae powder; B, preparing the solid phase extraction columns, namely putting C18 fillers into Solid Phase Extraction (SPE) columns; C, extracting algae toxin, namely weighing the dried algae powder, adding methanol according to a proportion, putting on a shaking table and oscillating at room temperature; D, removing impurities, namely adding a supernatant and an organic extractant into a separating funnel according to a proportion, mixing and uniformly shaking, standing, and after delamination, removing the organic extractant; E, separating and purifying the algae toxin, namely after pretreatment, enriching sample solution by using pre-activated series-connected SPE columns, and eluting the toxin by using 20 to 50 percent methanol aqueous solution; and F, performing Methyl Cellulose (MC) detection. The method has good separation effect, large loading amount, is easy and convenient to operate and low in cost, can obtain two microcystins with the weight of more than or equal to 10 mg and higher purity each time, and the High Performance Liquid Chromatography-Ultra Violet/Diode Array Detector (HPLC-UV/DAD) detection can reach 85 to 90 percent.

The invention discloses an application of biological selenium and glutathione as fish feed additives, wherein, the using amount of the biological selenium is that each kilogram of dry powder feed contains 0.5-1.0g of selenium-enriched yeast, and the using amount of the glutathione is that each kilogram of the dry powder feed contains 0.8-1.2g of the glutathione. The fish feed which is prepared by adding the biological selenium and the glutathione according to the application can promote the expression of detoxification genes of fish, especially the expression of I and II time phase metabolism detoxification-related genes, the fish feed can not only enhance the ability of resisting the outside poisons of the fish, but also be conductive to the decomposition and the removal of microcystins and drug toxins of the fish by the self-metabolic system, thereby ensuring the safety of eating the fish; the fish feed does not need to carry out the special treatment on the cultured aquatic products and does not need any special processing device, thereby greatly reducing the post-processing costs of the edible fish and being more environment-friendly and economic.

The invention discloses a method for inducing a rat liver cancer model by injecting diethyl nitrosamine DEN in coordination with microcystin MC-LR into a rat body through intraperitoneal injection. Indexes such as liver weight to body weight ratio, liver appearance, liver histopathological slicing and GST-Pi (Glutathione S-transferase) protein expression level clearly show liver cell injury phase, liver cell hyperplasia-hepatocirrhosis phase and liver cell carcinomatous change process in a rat liver cancer occurring process, and find that the liver cancer model induced by the DEN in coordination with the microcystin MC-LR has a more obvious effect at the same time than that induced by DEN independently; the tumor formation time is shorter; and the model is an ideal model for researching occurrence of the liver cancer of a human body. The model has the characteristic of clearly showing each stage for occurrence of the liver cancer and contributing to research of a liver cancer occurrence process mechanism, detection of precancerous lesion and development of treatment medicaments.

The invention provides a method for determining microcystic toxins concentration and is based on that molybdenum disulfide quantum dots can interact with gold nanoparticles through the internal filtration effect. Microcystic toxins can affect the degree of aggregation of aptamer-modified gold nanoparticles in the high salt solution, and detection of the microcystic toxins concentration in the water environment is achieved. The method is advantaged in that upconversion fluorescence of the molybdenum disulfide used can effectively avoid interference of background matrix fluorescence in the waterenvironment, optical stability is good, the detection result is accurate and reliable, cost is low, operation is simple, environmental protection is realized, detection specificity is good, influenceof other common metal ions or anions on determination of the microcystic toxins is avoided, the anti-interference ability is strong, the result is sensitive and reliable, the detection limit is low,the response speed is fast, the entire reaction process takes only 20 minutes, detection efficiency is greatly improved, good stability is achieved, real-time online fast, specific detection can be realized, and the method can be used for simple and rapid detection of microcystic toxins in the actual water environment.

The invention relates to a method for degradation of microcystin in water by means of electron beam irradiation and belongs to the technical field of sewage treatment. The method is a novel technique for microcystin removal in a source water or drinking water treatment procedure. Experimental processes confirm microcystin degradation effect of electron beam irradiation. 2-5kGy irradiation has evident inhibition and removing effects on generation of intracellular toxin, the extracellular toxin removal rates of the 2-5kGy irradiation are 76.9%, 76.2%, 76.2% and 97.7% respectively, and free radicals formed in solution during irradiation are absorbed by algae liquid so that toxins are effectively degraded. Special structures in the toxins are changed or removed by electron beam irradiation, and efficient degradation is achieved. The removal effect is increasingly evident along with increase of dose, and microcystin in algae containing water environments can be effectively removed and restrained from growing when irradiation dose is 5kGy.

The invention discloses a preparation method and an application of immobilized algal toxin degrading bacteria. The preparation method of the immobilized algal toxin degrading bacteria comprises the steps: selecting activated carbon fibers as an immobilization material, after carrying out washing, boiling, hydrochloric acid soaking, drying and other pretreatment steps of the immobilization material, adding into an LB culture medium according to a certain specific value, then inoculating a bacterial strain for degrading microcystins (MCs), and carrying out immobilized culture to obtain the immobilized algal toxin degrading bacteria. In the application of the immobilized algal toxin degrading bacteria, while the activated carbon fibers adsorb algal toxins, the algal toxin degrading bacteria immobilized on the activated carbon fibers can degrade the algal toxins, can greatly shorten the time of degradation of the MCs, and simultaneously can biologically regenerate the activated carbon fibers. The bacterial strain for degrading the MCs has the MC-LR degradation rate of up to 91% or more, and is especially suitable for degradation of the MCs generated in a process of extracting phycobiliproteins from water-blooming cyanobacteria.

The present invention relates to a selective photoelectrochemical analysis method for microcystin MC-LR in water. According to the present invention, graphene is chemically bonded to TiO2NTs through clicking chemistry so as to substantially improve the photoelectrochemical performance of the base electrode, the graphene modified TiO2NTs electrode and a MC-LR nucleic acid aptamer construct a MC-LR photoelectrochemical aptamer sensor, and the standard working curve of MC-LR is determined by using the photoelectrochemical analysis method, such that the MC-LR concentration in the sample to be detected can be detected by detecting the photocurrent density of the sample to be detected and combining the standard working curve; and compared to the method in the prior art, the method of the present invention has advantages of high detection sensitivity, simple detection method, high selectivity, and the like.

The invention relates to a method for improving efficiency of removing microcystin by probiotics, which belongs to the technical field of food organisms. The fermentation liquor which is abundant in probiotics is prepared by adopting the probiotics as a biological catalyst through liquid deep fermentation or the fermentation liquor is refined to obtain bacterial mud; the obtained probiotic fermentation liquor or the bacterial mud is charged into water; meanwhile, glucose as an external additive is placed to carry out vibration culture at 37 DEG C for 24-30 hours by 150rpm, and the removing efficiency of the probiotics on the microcystin is obviously improved. Because the glucose and the probiotics have no harm to the safety of humans and livestock and have the health effect, the invention can be widely applied to the field of foods, and a healthy and safe product with rich nutrient components is obtained.

Preparation and use methods of a microcystin-LR (MC-LR) monoclonal antibody immunoaffinity column (IAC) belong to the technical field of immunoaffinity chromatography and MC detection. The IAC is prepared by fixing MC-LR monoclonal antibody in the column, and can acquire the accurate contents of MC-LR and MC-RR through solvent extraction of cyanobacteria sample, enrichment of a solid-phase extraction column, IAC purification and quantitative determination by liquid chromatography-mass spectrometry. The determination can be interfered seriously by impurities to influence the result accuracy by only using SPE purification without IAC purification. The methods can be used for MC detection in water sample, other algae and similar biological samples.

Method of removing and purifying microcystin in drinking water belongs to water treatment technology field. The invention uses ozone oxidation-activated carbon fiber combination to remove algae toxin in drinking water: 1) taking water from water source and reducing COD, SS regualr indicators in water source by pretreatment of mixed coagulating, depositing and filtering; 2) using ozone oxidation method to remove microcystin and reduce DOC in water, using jet mixing technology in the treatment to react completely, dosage of liquid ozone is 1-5 mg/L, pH 7.0, reacting at room temperature for 30 minutes to obtain more than 95% removal rate of microcystin and more than 80% DOC degradation rate; 3) after ozone oxidation the discharged water entering activated carbon fiber treatment process, using activated carbon fiber absorbtion method to remove incompletely degradated microcystin and small molecular organic compounds brought by ozone oxidation; the discharged water meeting the standard of national 2006 GB5749-2006.

The invention discloses a fluorescent probe for detecting microcystic toxin-LR in water, which belongs to the field of molecular biology and microbiology. According to the fluorescent probe, MC-LR is taken as a target, a dodecapeptide library randomly shown on the surface of a bacteriophage is used for selecting a recombination bacteriophage which is combined with the MC-LR in a specific manner, and the single-stranded DNA (Deoxyribonucleic Acid) of the recombination bacteriophage is extracted and recombined for sequencing and sequence alignment, thereby obtaining an MC-LR specific affinity ligand sequence: His-Phe-Phe-Lys-Trp-His-Thr-Arg-Thr-Asn-Asp-Gln, and subsequently, obtaining the specifically combined fluorescent probe FITC-Acp-His-Phe-Phe-Lys-Trp-His-Thr-Arg-Thr-Asn-Asp-Gln through solid-phase polypeptide synthesis and fluorescent marks. The fluorescent probe has the beneficial effects that firstly, a completely new tool is provided for the specific identification and content detection of the MC-LR; and secondly, the fluorescent ligand probe can be used for qualitatively identifying the MC-LR and quantitatively detecting the content of the MC-LR in a sample.

The invention discloses a rapid detecting sensor for microcystins. The rapid detecting sensor utilizes a photochemistry polymerization method or an electrochemical polymerization method to synthesize a molecularly imprinted membrane, so that the combining capacity is improved, the delay time is reduced, and the specific adsorption capacity is strengthened; meanwhile, a Lamb wave piezoelectric membrane is utilized as an effector of the sensor, so that the sensitivity of the sensor is improved, the stability is increased, the cost is reduced, the performance of the sensor is strengthened, the trace detection of the microcystins is realized, and thus the rapid detecting method for the microcystins, with high sensitivity, rapidness, low cost and low operation requirements, is established.