26 results about "Microcystin" patented technology

The invention provides a preparation method and application of a high-sensitivity imprinted membrane for selectively detecting microcystic toxins (MC-LR) in water based on surface enhanced Raman scattering. The preparation method comprises the following steps: step 1, preparation of silver nanocubes; step 2, preparation of Ag NCs-coated TiO2 nanoparticles; step 3, preparation of an Ag NCs-coated TiO2 / PDMS composite membrane; according to the invention, a membrane technology, an SERS technology and MIT are combined, so that the prepared product has excellent physical and chemical properties of a PDMS membrane, high sensitivity of an SERS detection technology and high selectivity of MIT; the invention provides a new method for SERS detection and widens the application range.

The invention relates to a bio-based adsorbent capable of removing microcystic toxins-LR in water, a preparation method of the bio-based adsorbent comprises the following steps: performing steam explosion treatment on rice husks, and then jointly treating the steam-exploded rice husks with sodium hydroxide and sodium trimetaphosphate to obtain rice husks containing phosphate ester groups. The prepared bio-based adsorbent can realize adsorption of microcystic toxins-LR in wastewater, and also has efficient adsorption capacity in the presence of humic acid, calcium carbonate, sodium chloride, magnesium sulfate and other organic and inorganic substances. The adsorption rate of the rice hull powder adsorbent subjected to joint modification on microcystic toxins-LR is obviously higher than that of an original rice hull powder adsorbent, and the rice hull powder adsorbent can quickly adsorb pollutants in water and has a good removal effect on the microcystic toxins-LR within the pH range of an algae culture medium; the potential of adsorbing the microcystic toxins-LR in a microalgae culture environment is realized. Meanwhile, as a typical agricultural waste, the rice husks are turned into wealth through a two-step rapid modification method, and a new way for recycling the rice husks is provided. Therefore, the method for removing the microcystic toxins-LR in the water by utilizing the biomass adsorbent is a good choice, so that the pollution removal of the water body and the reutilization of agricultural wastes are realized.

The invention relates to a preparation method of a multi-energy coupling water body shading diaphragm plate and the shading diaphragm plate, and belongs to the field of water environment treatment. The preparation method comprises the following steps: preparing outer-layer functional shell slurry consisting of PVDF (Polyvinylidene Fluoride), Fe3O4 and SiO2 aerogel; preparing middle-layer light absorption layer slurry which is composed of nano WO3, nitrogen defect g-C3N4 and Fe3O4 and is filled with nitrogen to regulate and control the density; preparing inner-layer super-hydrophilic layer slurry formed by modified TiO2 doped with Fe < + > and V2O5; and sequentially coating the three layers of slurry by adopting a blade coating method, pre-drying, and curing and forming in an alkaline solution in a mold with a pit structure. The prepared shading diaphragm plate has a three-layer structure of an outer-layer functional shell, a middle-layer regulation and control and an inner-layer functional core, can synchronously realize efficient shading and algal inhibition, microcystin degradation and magnetic control recovery, has the advantages of wide scene adaptability and long service life, and is suitable for algal bloom treatment and pollution regulation and control of static and flowing water bodies.

The invention belongs to the technical field of small molecule detection, and relates to a nucleic acid-based algal toxin on-site rapid quantitative detection paper chip device and a detection method thereof. A reaction area of the paper chip device is coated with an aptamer c-DNA and a fluorescent group labeled-DNA, and a reading area of the paper chip device is coated with a capture nucleic acid probe d-DNA; the nucleic acid sequence of the aptamer c-DNA is as shown in SEQ ID NO.1, and the 5'end of the c-DNA is marked with biotin; the nucleic acid sequence of the capture nucleic acid probe is shown as SEQ ID NO.1, the nucleic acid sequence of the capture nucleic acid probe is shown as SEQ ID NO.2, the 5'end of the capture nucleic acid probe is marked with an FAM fluorophore, the nucleic acid sequence of the capture nucleic acid probe d-DNA is shown as SEQ ID NO.3, and the 5 'end of the capture nucleic acid probe d-DNA is marked with biotin. The whole detection process does not exceed 10 minutes, the cost is low, and rapid on-site quantitative detection of algal toxin in water is achieved.

The invention relates to the technical field of pollution treatment, and particularly discloses a composite bio-enzyme preparation for polluted water treatment and a preparation method of the composite bio-enzyme preparation. The invention relates to a composite biological enzyme preparation for polluted water treatment. The composite biological enzyme preparation comprises the following raw materials in parts by weight: 25-30 parts of an amylolytic enzyme compound, 20-25 parts of a proteolytic enzyme compound, 15-20 parts of lipase, 10-15 parts of cellulose hydrolase, 15-20 parts of flora freeze-dried powder, 10-15 parts of porous calcium alginate microspheres, 5-8 parts of chitosan modified silicon dioxide and 3-5 parts of an enzyme stabilizer. According to the application, the content of total microcystic toxins and the content of refractory organic matters in the polluted water can be obviously reduced, the sewage treatment effect is facilitated, and secondary pollution in the sewage treatment process is reduced.

The invention discloses a method for removing microcystis aeruginosa, which comprises the following steps: (1) adding peracetic acid into a microcystis aeruginosa water body, so that the concentration of the peracetic acid in the water body is 0.1-5.0 mmol / L; and (2) carrying out electrolytic reaction on the water body in the step (1) by adopting an electrochemical oxidation device, activating peracetic acid in the water body, and enabling generated free radicals to act on microcystis aeruginosa cells so as to remove the microcystis aeruginosa. According to the method, pseudomonas aeruginosa can be rapidly removed, the degradation rate within 30 minutes exceeds 90%, microcystin-LR is eliminated, and the biotoxicity is remarkably reduced.

The invention discloses a time-resolved fluorescent quantitative detection microcystin test strip as well as a preparation method and a detection method of the time-resolved fluorescent quantitative detection microcystin test strip. The test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are arranged on a bottom plate. A combination pad of the test strip is coated with a europium-labeled microcystin nucleic acid aptamer nanoprobe, and a nitrocellulose membrane is provided with a detection line and a quality control line. During detection, microcystic toxins in a sample are competitively combined with the probe to influence a T line fluorescence signal, and accurate quantification can be realized by measuring the fluorescence intensity ratio of a T / C line and substituting the fluorescence intensity ratio into a standard curve. The high specificity of the nucleic acid aptamer and the high sensitivity of time-resolved fluorescence are combined, and the probe structure, the reagent formula and the preparation process are optimized, so that the detection has the advantages of high sensitivity, strong specificity, rapidness, simplicity and convenience in operation, good stability and the like, and is suitable for on-site rapid detection of microcystin in a water environment and an algae sample.

The invention discloses a nano-microsphere time-resolved fluorescent probe as well as a preparation method and application thereof. The preparation method comprises the following three steps: preparation of core-layer nano microspheres, shell-layer functional modification and fluorescence enhancement and stabilization treatment. A polylactic acid-glycolic acid copolymer (PLGA) is used as a core-layer carrier to load a rare earth ion complex, and a SiO2 coating layer and a targeting ligand are combined for modification, so that the nano-microsphere fluorescent probe with a core-shell structure is prepared. The probe has the characteristics of long fluorescence lifetime (5-8ms), high quantum yield (greater than or equal to 35%), strong stability, excellent targeting property and the like, and can be widely applied to food safety detection (such as quinolone antibiotics and microcystin MC-LR) and environmental pollution detection (such as heavy metal ions such as Hg < 2 + > and Pb < 2 + >), and the detection limit is as low as 0.05 ng / mL or 0.1 nmol / L. The problems that a traditional fluorescent probe is low in loading capacity, poor in stability and insufficient in targeting property are solved, and the method has remarkable industrial application value.

The application discloses a method for efficiently removing algae based on activation of calcium peroxide by trace copper ions, and under the conditions that the Cu(II) dosage is only 15 micromoles, the Cu(II) / CaO2 molar ratio is 1:7, and the pH is neutral, the removal rate of algae cells is higher than 97% for the algae with a concentration of 1.0-2.5*10 6 cells per milliliter. ‑1 The removal rate of algae cells is higher than 97%. The system utilizes the alkalinity of CaO2 to promote the cycle of Cu(II) / Cu(I), generates more Cu(III) and active free radicals such as ∙OH, ∙O2 ‑ 、 1 O2, significantly improves the oxidation and algae removal ability of the system; the mild oxidizing property of CaO2 can inhibit the direct attack of copper ions on algae cells, and reduces the release risk of dissolved algae organic matter and microcystin; after the reaction, Ca(OH)2 generated by the hydrolysis of CaO2 can simultaneously precipitate the algae cells and copper ions in the water body, so that the residual copper concentration is reduced to 30.1 micromoles per liter. ‑1 The following effectively avoids the risk of secondary pollution of heavy metals. That is, the coupling system successfully overcomes the defects of traditional H2O2, such as fast decomposition, narrow pH application range, inconvenient transportation and the like, and has the characteristics of high algae removal efficiency, strong adaptability, environmental friendliness and the like, and has good popularization and application value.

A water body algae toxin concentration estimation method and system based on remote sensing image belong to the technical field of inland water body eutrophication lake water environment evaluation, solve the technical problems that the existing technology has high precision requirement of detection instrument, expensive equipment material, complex and low efficiency of water sample pretreatment process, and the remote sensing indirect inversion method has poor accuracy and is difficult to realize accurate quantification of microcystin. Step 1, obtain the remote sensing image of the water body, and pretreat to obtain the remote sensing reflectivity data; step 2, calculate the spectral index based on the remote sensing reflectivity data; step 3, construct a water body microcystin concentration remote sensing estimation model, take the spectral index as the input of the water body microcystin concentration remote sensing estimation model, and obtain the water body microcystin concentration. The present application is used for realizing efficient and accurate water body algae toxin concentration estimation.

The application discloses a Pseudomonas capable of degrading microcystins, an immobilized bacterial agent and application, and particularly relates to Pseudomonas chengduensis DMC-X1, which is isolated and screened from surface water samples of a cyanobacterial bloom outbreak in Nanyi Reservoir in Nanjing County, Zhangzhou City, Fujian Province. The strain belongs to gamma-proteobacteria, has high degradation capacity for microcystins (MCs), and has a degradation capacity superior to most Pseudomonas reported at present, and can be used for treating water polluted by microcystins. In the application, sodium alginate is used as a carrier, CaCl2 is used as a crosslinking agent, and the Pseudomonas DMC-X1 is embedded to obtain an immobilized bacterial agent, which can degrade microcystins in a short time and can be recycled for multiple times. The immobilized bacterial agent is convenient to store and recycle, and has important application value.

The invention discloses a method for extracting and detecting microcystic toxins in water, and belongs to the technical field of water quality detection.The method comprises the steps that cyclodextrin and a compound with a quaternary ammonium salt structure are polymerized, and a porous cyclodextrin polymer with cations is obtained and serves as a cation modified solid-phase extraction column; the covalently-bound and non-covalently-bound microcystic toxins are converted into free microcystic toxins through enzymolysis and the cation modified solid-phase extraction column, the recovery rate is high, and the detection accuracy is improved.

The invention relates to an aggregation-induced electrochemical luminescence microcystic toxin-LR sensing platform based on a supramolecular organic framework, and provides a preparation and detection method of the platform. The invention particularly relates to preparation of the aggregation-induced electrochemiluminescence microcystic toxin-LR sensing platform based on the supramolecular organic framework and a detection method of the aggregation-induced electrochemiluminescence microcystic toxin-LR sensing platform based on the supramolecular organic framework. A subject-object recognition and supramolecular constraint enhanced AIECL strategy is adopted, and CB [8] is selected as a host molecule. The molecular movement of TPE-CEPy is limited through the host-guest binding effect, and a TPE-CEPy-CB [8] composite system is successfully constructed. The material is constructed based on a subject-object recognition mechanism, and the luminous efficiency and stability of the AIECL are remarkably improved. The invention belongs to the field of analytical chemistry.

The invention discloses a feed additive composition for relieving microcystin toxicity and preparation and application thereof.The composition is composed of eucommia ulmoides extract, astragalus membranaceus extract, radix bupleuri extract and macleaya cordata extract.The plant extract composition is added into feed, so that the growth performance of aquatic organisms can be promoted, the oxidation resistance and immunity are enhanced, and the microcystin toxicity can be relieved. The survival rate under the stress of the microcystic toxins is improved. The raw materials are non-medication parts of Chinese herbal medicines, and are low in price, ecological, environment-friendly, healthy and pollution-free.

The invention provides a preparation method of an organic photoelectrochemical transistor sensing platform for detecting microcystic toxins, which comprises the following steps: preparing a polydopamine nanosphere through a dopamine hydrochloride self-assembly reaction, and carrying out modification of a microcystic toxin second antibody and sealing treatment of bovine serum albumin to obtain the organic photoelectrochemical transistor sensing platform for detecting the microcystic toxins. According to the method, a microcystic toxin detection platform with high sensitivity and good selectivity is constructed by utilizing the specific binding capacity of the polydopamine nanospheres and the high sensitivity characteristic of the organic photoelectrochemical transistor; a new technical means is provided for the fields of environment monitoring, food detection and the like, the prepared organic photoelectrochemical transistor sensing platform has good selectivity and stability on microcystic toxins, and the detection limit is 18 pg / mL.

The invention relates to an SERS (Surface Enhanced Raman Scattering) imprinted membrane constructed on the basis of a photo-initiation technology, preparation of the SERS imprinted membrane and application of the SERS imprinted membrane to selective detection of microcystic toxins in water. The preparation method comprises the following steps: step 1, preparation of silver nanocubes; step 2, preparation of Ag NCs-coated Cu2O nanoparticles; step 3, preparation of an Ag NCs-coated Cu2O / PDMS (Polydimethylsiloxane) composite membrane; according to the invention, a membrane technology, an SERS technology and MIT are combined, so that the prepared product has excellent physical and chemical properties of a PDMS membrane, high sensitivity of an SERS detection technology and high selectivity of MIT; the invention provides a new method for SERS detection and widens the application range.

The invention discloses a water algae detection device based on a GaN chip and a detection method for water algae thereof.The device is formed by integrating a microcystin sensor, a chlorophyll sensor and an ammonium ion sensor which share a substrate layer and a cover plate, and the structure of each unit sequentially comprises a gallium nitride layer, an aluminum gallium nitrogen barrier layer and a gallium nitride cap layer from bottom to top; a source electrode, a drain electrode and a grid electrode region are arranged on the gallium nitride cap layer in parallel, a sensing film with a microcystin and chlorophyll a combination function and an organic molecular polymer serving as an ammonium ion sensing film are deposited on the surface of the grid electrode region of each unit, and the aluminum gallium nitrogen barrier layer and the gallium nitride layer in the sensor form a heterojunction. Two-dimensional electron gas is formed in the channel and is sensitive to potential change of the surface of the grid electrode, and compared with a traditional water algae detection means, the method is more portable, detection is rapid, and the result is accurate.

The application belongs to the technical field of electrochemical detection and analysis, and particularly relates to a light-assisted enhanced self-powered electrochemical sensor for detecting microcystin-RR (MC-RR) and a preparation method thereof. 18 O 49 A heterojunction is further formed with Nb2C MXene. Polarization of the molecule can be achieved by doping Mo atoms into W 18 O 49 , which promotes electron transfer. The Fermi level of Nb2C MXene is lower, and the conductivity is higher. The application widens the application of W 18 O 49 in self-powered photoelectrochemical sensors, and the prepared sensor has a wide detection range, high sensitivity and low detection limit, and is of great significance for detecting MC-RR.

The invention belongs to the technical field of microorganisms, and particularly relates to a novel sphingosine bacterium as well as strain fermentation liquor and application thereof. The invention discloses a novel sphingosine bacterium which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.28677. The invention further discloses a preparation method of the novel sphingosine bacterium. The novel sphingosine bacterium has efficient degradation capacity on microcystin, and the highest degradation rate is close to 100%. Neosphingosine bacteria and a bacterial strain fermentation broth thereof can be used as a microcystin degradation preparation for repairing a microcystin polluted environment.

The application discloses a microcystin degradation agent in water, a preparation method and application thereof, and utilizes oxalic acid and siderite to prepare a compound oxalic acid degradation agent, the degradation agent can degrade microcystin and antibiotic pollutant cefazolin sodium in water, and the degradation reaction can be carried out in dark light conditions, meanwhile, the reaction pH has a wide range, and the degradation can be carried out at pH 5-9; the degradation agent can degrade more than 93% of the microcystin within 26 hours, and the degradation rate of the cefazolin sodium can reach more than 93% within 10 hours, which indicates that the degradation agent prepared by compounding oxalic acid on the surface of the siderite can be applied to the degradation and removal of microcystin and organic pollutants such as antibiotics in water without external light, and therefore has wide application value.

This invention discloses a method for retrieving microcystin content in water using algal fluorescence spectroscopy. First, fluorescence spectral data of N algal solution samples with different concentrations are measured, and after preprocessing, the data are converted into a one-dimensional array M of length p. p Furthermore, all the corresponding one-dimensional arrays are combined into a matrix X. N The microcystin content of N algal solution samples was determined, forming a matrix Y. N Based on matrix X N With matrix Y N Establish model M ridge The fluorescence spectrum data of the sample to be tested were measured, and after preprocessing, they were converted into a one-dimensional array M of length p. px This is further transformed into matrix X. p Substitute into the established model M ridge In the model, the predicted values ​​of microcystin are automatically provided. Model M ridge During the modeling process, factors affecting fluorescence spectroscopy and algal growth can be incorporated to further improve model accuracy. This method is more accurate, simple, quick, and convenient, facilitating on-site monitoring and possessing significant practical value.

This invention discloses a method for establishing Alzheimer's disease in tree shrews. The specific steps involve mixing an ethanol solution of 0.5 μg / μl Microcystin-LR with a muscarine solution, and then injecting 2 μl into each of the bilateral Meynert basal ganglia of the tree shrew. After administration, the tree shrew is fed normally for two weeks to complete the Alzheimer's disease model. The muscarine solution is prepared under sterile conditions by adding 200 μl of sterile saline to a vial containing 1 mg of muscarine, repeatedly pipetting to ensure complete dissolution, resulting in a concentration of 5 μg / μl. The Microcystin-LR solution is a commercially available product. This invention involves injecting a mixture of 0.1 μg MC-LR and 3 μg IBO solution into the bilateral Meynert basal ganglia of tree shrews, establishing a stable tree shrew AD model after two weeks. Three days after administration, tree shrews exhibited AD behavioral manifestations, such as lethargy and reduced activity. Two weeks later, the tree shrew brains showed typical AD pathological manifestations, including Aβ deposition and elevated expression levels at multiple phosphorylation sites of Tau protein.

The invention relates to an aptamer for specifically recognizing tetrodotoxin and application of the aptamer, and belongs to the technical field of detection. The cyclic divalent aptamer disclosed by the invention has good affinity and specificity and good thermal stability, is not interfered by impurities such as ion strength and protein in globefish meat and serum during tetrodotoxin detection, and has high conformational stability. Based on the annular bivalent aptamer, the invention further designs an aptamer sensor based on a 3D-DNA walker, the aptamer sensor can specifically detect low-concentration tetrodotoxin, the detection limit is 0.02 ng / mL, the detection time is 1 h, and the detection sensitivity is high. Other marine toxins such as gonyautoxin (GTX), dinophysiotoxin (DTX), sea anemone toxin II (ATX-II), anabaena toxin-a (ATX-a) and microcystin (MC-LR) cannot be detected, and the specificity is good.

The application provides a high-specificity detection microcystin whole column-SERS / fluorescence dual-mode detection platform and a construction method thereof. The platform takes a three-dimensional porous amino silica gel monolithic column as a carrier, solidifies specific recognition probes through electrostatic adsorption, constructs the whole column-SERS / fluorescence dual-mode detection platform through base complementary pairing of cDNA and aptamers on the specific probe of Au@Ag@cDNA / Aptamer-Cy3, and utilizes the Au@Ag core-shell structure to generate SERS 'hot spot' enhancement effect. When target algae toxin molecules exist in the sample, the aptamers are specifically combined with the target substances and separated from the monolithic column, so that the base SERS signal is weakened and the fluorescence signal of the effluent is enhanced, high-sensitivity and high-specificity dual-mode qualitative or quantitative detection of microcystin is realized through the dual signal changes, and the detection sensitivity and accuracy are improved.

The application discloses a Paucibacter psychrotolerans capable of degrading microcystins and application thereof. The Paucibacter psychrotolerans provided by the application is Paucibacter psychrotolerans 2WFC-1, and the preservation number thereof in the China General Microbiological Culture Collection Center is CGMCC No. 24793. The strain 2WFC-1 belongs to a new species of the Paucibacter genus, can degrade microcystins, and is different from other Paucibacter in that it can grow at a temperature ranging from 4 DEG C to 37 DEG C, has great potential for degrading microcystins in a low-temperature water environment, and has important application prospects in water ecological restoration, seasonal water pollution treatment and drinking water purification.