58 results about "Microcystin" patented technology

The invention discloses a microcystis fungus, an anti-inflammatory and anti-osteoporosis compound as well as a preparation method and application of the microcystis fungus and the anti-inflammatory and anti-osteoporosis compound. According to the invention, two cyclic ester peptide compounds, i.e., Microascusin A (formula I) and Microascusin B (formula II), with new structures, are obtained from sponge-derived fungus Microascusin, i.e., Microascusin A (formula I) and Microascusin B (formula II), and the microascusin A (formula I) and Microascusin B (formula II) are obtained from sponge-derived fungus Microascusin. The microcystin A and the microcystin B are natural products obtained through microbial fermentation, the raw materials are easy to obtain, and the microcystin A and / or the microcystin B and the pharmaceutical composition can be used for preventing and treating inflammation and / or osteoporosis. The microcystin A and / or microcystin B can also be combined with known medicines to form a compound preparation for preventing and treating inflammation and / or osteoporosis. # imgabs0 #

The invention discloses a tree shrew Alzheimer's disease modeling method. The method comprises the following specific steps: mixing an ethanol solution of Microcystin-LR with the concentration of 0.5 [mu] g / [mu] l with an amaninic acid solution, and injecting 2 [mu] l to Meynert basal nuclei on the two sides of a tree shrew respectively; and after drug administration is finished, normally feeding for 2 weeks to finish Alzheimer's disease modeling. The method comprises the following steps: under a sterile condition, sucking 200 microliters of sterile normal saline, adding the sterile normal saline into a small bottle containing 1mg of amanitine, repeatedly blowing and beating to completely dissolve the amanitine, and preparing a solution with the concentration of 5mu g / microliter; the Microcystin-LR solution is a product purchased in the market. According to the invention, a mixed solution of MC-LR (0.1 [mu] g) and IBO solution (3 [mu] g) is respectively injected to Meynert basal nuclei on both sides of the tree shrew, and a stable tree shrew AD model can be established after 2 weeks. After the medicine is administrated for 3 days, tree shrews have AD behavior expressions, such as drooping spirit and reduced activity; after two weeks, the tree shrew brain shows AD typical pathological expressions such as A beta deposition, increase of expression levels of multiple phosphorylation sites of Tau protein and the like.

The invention provides a preparation method and application of a high-sensitivity imprinted membrane for selectively detecting microcystic toxins (MC-LR) in water based on surface enhanced Raman scattering. The preparation method comprises the following steps: step 1, preparation of silver nanocubes; step 2, preparation of Ag NCs-coated TiO2 nanoparticles; step 3, preparation of an Ag NCs-coated TiO2 / PDMS composite membrane; according to the invention, a membrane technology, an SERS technology and MIT are combined, so that the prepared product has excellent physical and chemical properties of a PDMS membrane, high sensitivity of an SERS detection technology and high selectivity of MIT; the invention provides a new method for SERS detection and widens the application range.

The growth of algae can be controlled in water by exposing water containing algae to agitation, for example shear stress and an electrical field. The water may further be detoxified of the microcystins produced by algae by treating the water with a microcystin-degrading bacterium, such as Sphingomonas sp. together with an electric field, with the application of the electric field causing a major acceleration of the toxin's degradation.

The invention relates to a bio-based adsorbent capable of removing microcystic toxins-LR in water, a preparation method of the bio-based adsorbent comprises the following steps: performing steam explosion treatment on rice husks, and then jointly treating the steam-exploded rice husks with sodium hydroxide and sodium trimetaphosphate to obtain rice husks containing phosphate ester groups. The prepared bio-based adsorbent can realize adsorption of microcystic toxins-LR in wastewater, and also has efficient adsorption capacity in the presence of humic acid, calcium carbonate, sodium chloride, magnesium sulfate and other organic and inorganic substances. The adsorption rate of the rice hull powder adsorbent subjected to joint modification on microcystic toxins-LR is obviously higher than that of an original rice hull powder adsorbent, and the rice hull powder adsorbent can quickly adsorb pollutants in water and has a good removal effect on the microcystic toxins-LR within the pH range of an algae culture medium; the potential of adsorbing the microcystic toxins-LR in a microalgae culture environment is realized. Meanwhile, as a typical agricultural waste, the rice husks are turned into wealth through a two-step rapid modification method, and a new way for recycling the rice husks is provided. Therefore, the method for removing the microcystic toxins-LR in the water by utilizing the biomass adsorbent is a good choice, so that the pollution removal of the water body and the reutilization of agricultural wastes are realized.

The invention relates to the field of bioanalytical chemistry and environmental monitoring, and discloses a nano fluorescent probe, a preparation method and application thereof, and a method for detecting microcystic toxins in liquid. The nano composite probe comprises an MOF material and nano particles loaded on the MOF material, the nano particles comprise carboxyl-modified up-conversion nano particles and nucleic acid aptamers covalently connected with the up-conversion nano particles through amido bonds, and the up-conversion nano particles contain a thulium element and an erbium element. The nano fluorescent probe has the advantages of high specificity, simplicity and rapidness in operation, high sensitivity, high interference resistance, simple preparation process and low cost, provides an efficient solution for rapid detection of liquid microcystin pollution, and has a remarkable market prospect.

The invention relates to a preparation method of a multi-energy coupling water body shading diaphragm plate and the shading diaphragm plate, and belongs to the field of water environment treatment. The preparation method comprises the following steps: preparing outer-layer functional shell slurry consisting of PVDF (Polyvinylidene Fluoride), Fe3O4 and SiO2 aerogel; preparing middle-layer light absorption layer slurry which is composed of nano WO3, nitrogen defect g-C3N4 and Fe3O4 and is filled with nitrogen to regulate and control the density; preparing inner-layer super-hydrophilic layer slurry formed by modified TiO2 doped with Fe < + > and V2O5; and sequentially coating the three layers of slurry by adopting a blade coating method, pre-drying, and curing and forming in an alkaline solution in a mold with a pit structure. The prepared shading diaphragm plate has a three-layer structure of an outer-layer functional shell, a middle-layer regulation and control and an inner-layer functional core, can synchronously realize efficient shading and algal inhibition, microcystin degradation and magnetic control recovery, has the advantages of wide scene adaptability and long service life, and is suitable for algal bloom treatment and pollution regulation and control of static and flowing water bodies.

The present application relates to the technical field of organic matter detection, and specifically discloses a pretreatment process and application of microcystin in sediments. A pretreatment process for microcystin in sediments, comprising the following steps: drying and grinding the sediment; dispersing the pretreated sediment into a solvent and then adding a modified adsorbent, ultrasonicating, filtering to obtain an extract A and a precipitate; washing the precipitate with acetone, taking out the modified adsorbent, adding it to the solvent, heating for desorption, and filtering to obtain an extract B; mixing the extract A and the extract B to obtain a crude extract; the preparation method of the modified adsorbent is: adding tannic acid and a porous material to a chitosan solution, stirring and mixing, then adding a cross-linking agent, stirring and mixing, to obtain; the crude extract is passed through a column and eluted. Adding a modified adsorbent to this process can increase the extraction amount of MC, which is beneficial to the detection of trace microcystin in sediments and reduces the influence of impurities such as proteins on the detection.

The invention discloses preparation of urea-derived carbon dots and application of the urea-derived carbon dots to inhibition of algae and degradation of algal toxins, and belongs to the technical field of nano agricultural regulation and control. Urea is used as a substrate, and urea-derived carbon dots (UDs) are synthesized through a hydrothermal method. The UDs can be used as an inhibitor to inhibit growth of algae cells in lakes, and can also be used as a photocatalyst to generate free radicals to attack the algae cells, so that the oxidative stress level and membrane damage degree of the algae cells are increased to cause death of the algae cells, and microcystic toxins generated by the UDs are degraded at the same time.

The invention belongs to the technical field of small molecule detection, and relates to a nucleic acid-based algal toxin on-site rapid quantitative detection paper chip device and a detection method thereof. A reaction area of the paper chip device is coated with an aptamer c-DNA and a fluorescent group labeled-DNA, and a reading area of the paper chip device is coated with a capture nucleic acid probe d-DNA; the nucleic acid sequence of the aptamer c-DNA is as shown in SEQ ID NO.1, and the 5'end of the c-DNA is marked with biotin; the nucleic acid sequence of the capture nucleic acid probe is shown as SEQ ID NO.1, the nucleic acid sequence of the capture nucleic acid probe is shown as SEQ ID NO.2, the 5'end of the capture nucleic acid probe is marked with an FAM fluorophore, the nucleic acid sequence of the capture nucleic acid probe d-DNA is shown as SEQ ID NO.3, and the 5 'end of the capture nucleic acid probe d-DNA is marked with biotin. The whole detection process does not exceed 10 minutes, the cost is low, and rapid on-site quantitative detection of algal toxin in water is achieved.

The invention discloses a method for efficiently removing algae based on trace copper ion activated calcium peroxide, and under the conditions that the addition amount of Cu (II) is only 15 mu M (1 mgL <-1 >), the molar ratio of Cu (II) to CaO2 is 1: 7, and the pH is neutral, the removal rate of algae cells with the concentration of 1.0-2.5 * 10 < 6 > cell smmL <-1 > is higher than 97%. According to the system, the circulation of Cu (II) / Cu (I) is promoted by utilizing the alkalinity of CaO2, more active free radicals such as Cu (III), OH, O2 <-> and < 1 > O2 are generated, and the oxidation algae removal capacity of the system is remarkably improved; the direct attack of copper ions on algae cells can be inhibited by the mild oxidizing property of CaO2, and the release risk of soluble algae organic matters and microcystic toxins is reduced; ca (OH) 2 generated by hydrolysis of CaO2 after the reaction can synchronously precipitate algae cells and copper ions in the water body, so that the concentration of residual copper is reduced to 30.1 mu gL <-1 > or below, and the risk of secondary pollution of heavy metals is effectively avoided. The coupling system successfully overcomes the defects that traditional H2O2 is fast in decomposition, narrow in pH application range, inconvenient to transport and the like, has the advantages of being high in algae removal efficiency, high in adaptability, environmentally friendly and the like, and has good application and popularization value.

The present invention discloses a method for extracting microcystin from Anabaena flos-aquae, comprising the following steps: (1) obtaining the Anabaena flos-aquae strain that produces microcystin; (2) crushing the Anabaena flos-aquae strain, adding a methanol solution, extracting microcystin under stirring, then performing centrifugation, collecting the supernatant, and evaporating the supernatant to obtain a crude extract; (3) preliminarily purifying the crude extract using a solid-phase extraction column to obtain a refined extract; (4) further purifying the refined extract using semi-preparative liquid chromatography to obtain a solution containing microcystin, and then evaporating and drying to obtain the microcystin. The method for extracting microcystin from Anabaena flos-aquae of the present invention has the advantages of stable properties of the toxin-producing strain, single toxin-producing component, high toxin content, good repeatability of extraction and purification, etc., and can obtain a microcystin standard product with high purity and high yield.

The invention relates to the technical field of pollution treatment, and particularly discloses a composite bio-enzyme preparation for polluted water treatment and a preparation method of the composite bio-enzyme preparation. The invention relates to a composite biological enzyme preparation for polluted water treatment. The composite biological enzyme preparation comprises the following raw materials in parts by weight: 25-30 parts of an amylolytic enzyme compound, 20-25 parts of a proteolytic enzyme compound, 15-20 parts of lipase, 10-15 parts of cellulose hydrolase, 15-20 parts of flora freeze-dried powder, 10-15 parts of porous calcium alginate microspheres, 5-8 parts of chitosan modified silicon dioxide and 3-5 parts of an enzyme stabilizer. According to the application, the content of total microcystic toxins and the content of refractory organic matters in the polluted water can be obviously reduced, the sewage treatment effect is facilitated, and secondary pollution in the sewage treatment process is reduced.

The growth of algae can be controlled in water by exposing water containing algae to agitation, for example shear stress and an electrical field. The water may further be detoxified of the microcystins produced by algae by treating the water with a microcystin-degrading bacterium, such as Sphingomonas sp. together with an electric field, with the application of the electric field causing a major acceleration of the toxin's degradation.

The invention discloses a method for removing microcystis aeruginosa, which comprises the following steps: (1) adding peracetic acid into a microcystis aeruginosa water body, so that the concentration of the peracetic acid in the water body is 0.1-5.0 mmol / L; and (2) carrying out electrolytic reaction on the water body in the step (1) by adopting an electrochemical oxidation device, activating peracetic acid in the water body, and enabling generated free radicals to act on microcystis aeruginosa cells so as to remove the microcystis aeruginosa. According to the method, pseudomonas aeruginosa can be rapidly removed, the degradation rate within 30 minutes exceeds 90%, microcystin-LR is eliminated, and the biotoxicity is remarkably reduced.

The invention discloses a chromatographic test strip and a method for detecting microcystic toxins, the chromatographic test strip is provided with a sample pad, a combination pad and a detection pad which are sequentially arranged along a chromatographic direction, and silver-gold composite nanoparticles with beacon molecules are stored in the combination pad. The surfaces of the silver-gold composite nanoparticles are coupled with a first antibody which can be specifically bound with the microcystic toxins, and an aptamer which is specifically bound with the microcystic toxins is stored in the detection pad; a sample to be detected is added to the sample pad, after the reaction is completed, the detection pad is irradiated with laser, a Raman signal of the detection pad is collected, and the content of microcystic toxins in the sample to be detected is calculated according to the Raman signal. The detection method provided by the invention can rapidly realize quantitative detection of microcystic toxins in water, and has the advantages of high detection sensitivity, strong specificity and good accuracy.

The present invention belongs to the technical field of treatment of bloom cyanobacteria, and specifically discloses a recombinant enzyme for degrading microcystin in cyanobacteria slurry and a preparation method thereof. The recombinant enzyme has a solubility-enhancing tag NusA linked to the N-terminus of microcystin-degrading enzyme MlrA and a histidine tag linked to the C-terminus. This recombinant enzyme is characterized by strong stability and high expression level, and can still stably exist and efficiently degrade microcystin in a complex environment with large temperature difference in cyanobacteria accumulation, large pH change, low dissolved oxygen, and high nitrogen and phosphorus. After being treated with the crude extract of the recombinant enzyme for 24 h, the degradation efficiency of microcystin in the accumulated cyanobacteria slurry reaches 89.2%.

The invention discloses a time-resolved fluorescent quantitative detection microcystin test strip as well as a preparation method and a detection method of the time-resolved fluorescent quantitative detection microcystin test strip. The test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are arranged on a bottom plate. A combination pad of the test strip is coated with a europium-labeled microcystin nucleic acid aptamer nanoprobe, and a nitrocellulose membrane is provided with a detection line and a quality control line. During detection, microcystic toxins in a sample are competitively combined with the probe to influence a T line fluorescence signal, and accurate quantification can be realized by measuring the fluorescence intensity ratio of a T / C line and substituting the fluorescence intensity ratio into a standard curve. The high specificity of the nucleic acid aptamer and the high sensitivity of time-resolved fluorescence are combined, and the probe structure, the reagent formula and the preparation process are optimized, so that the detection has the advantages of high sensitivity, strong specificity, rapidness, simplicity and convenience in operation, good stability and the like, and is suitable for on-site rapid detection of microcystin in a water environment and an algae sample.

The invention discloses a nano-microsphere time-resolved fluorescent probe as well as a preparation method and application thereof. The preparation method comprises the following three steps: preparation of core-layer nano microspheres, shell-layer functional modification and fluorescence enhancement and stabilization treatment. A polylactic acid-glycolic acid copolymer (PLGA) is used as a core-layer carrier to load a rare earth ion complex, and a SiO2 coating layer and a targeting ligand are combined for modification, so that the nano-microsphere fluorescent probe with a core-shell structure is prepared. The probe has the characteristics of long fluorescence lifetime (5-8ms), high quantum yield (greater than or equal to 35%), strong stability, excellent targeting property and the like, and can be widely applied to food safety detection (such as quinolone antibiotics and microcystin MC-LR) and environmental pollution detection (such as heavy metal ions such as Hg < 2 + > and Pb < 2 + >), and the detection limit is as low as 0.05 ng / mL or 0.1 nmol / L. The problems that a traditional fluorescent probe is low in loading capacity, poor in stability and insufficient in targeting property are solved, and the method has remarkable industrial application value.

The invention discloses a microcystic toxin degrading enzyme MlrA mutant and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The microcystic toxin degrading enzyme MlrA mutant is obtained by carrying out single-point mutation on the 179th or 284th amino acid residue of the amino acid sequence of the wild microcystic toxin degrading enzyme MlrA as shown in SEQ ID No.1; the single point mutation is that phenylalanine at the 179th site is mutated into tyrosine, and arginine at the 284th site is mutated into glutamic acid. Two genetically engineered bacteria for expressing the mutant microcystic toxin degrading enzyme MlrA are obtained through a genetic engineering technology, MC-LR is degraded by utilizing the genetically engineered bacteria, the service life of cells is greatly prolonged, and the requirements of current industrial application are met.

The invention provides a lake and reservoir water source microcystis rapid detection method and device, and the method specifically comprises the following steps: carrying a sampling detection module and an underwater camera assembly to enter a lake and reservoir water source by using an unmanned aerial vehicle; the sampling detection module is used for collecting microcystis at different depths in a lake and reservoir water source through a plurality of sampling boxes which are sequentially arranged from top to bottom; the sampling detection module is used for immediately filtering and enriching microcystic toxins; taking out the sampling piece, horizontally placing, washing with clean water, and placing under a microscope for observation; the water sample in the sampling box is filtered, impurities and residual microcystis in the water sample are filtered, and a concentrated microcystin solution is obtained; the pH value of the microcystin solution is adjusted, purification treatment is conducted, and then a liquid chromatograph and a mass spectrometer are combined for analysis and determination. According to the method and the device for rapidly detecting the microcystis in the lake and reservoir water source, the microcystis can be rapidly, efficiently and conveniently sampled, and rapid and efficient enrichment of microcystis toxins is also realized.

The application discloses a method for efficiently removing algae based on activation of calcium peroxide by trace copper ions, and under the conditions that the Cu(II) dosage is only 15 micromoles, the Cu(II) / CaO2 molar ratio is 1:7, and the pH is neutral, the removal rate of algae cells is higher than 97% for the algae with a concentration of 1.0-2.5*10 6 cells per milliliter. ‑1 The removal rate of algae cells is higher than 97%. The system utilizes the alkalinity of CaO2 to promote the cycle of Cu(II) / Cu(I), generates more Cu(III) and active free radicals such as ∙OH, ∙O2 ‑ 、 1 O2, significantly improves the oxidation and algae removal ability of the system; the mild oxidizing property of CaO2 can inhibit the direct attack of copper ions on algae cells, and reduces the release risk of dissolved algae organic matter and microcystin; after the reaction, Ca(OH)2 generated by the hydrolysis of CaO2 can simultaneously precipitate the algae cells and copper ions in the water body, so that the residual copper concentration is reduced to 30.1 micromoles per liter. ‑1 The following effectively avoids the risk of secondary pollution of heavy metals. That is, the coupling system successfully overcomes the defects of traditional H2O2, such as fast decomposition, narrow pH application range, inconvenient transportation and the like, and has the characteristics of high algae removal efficiency, strong adaptability, environmental friendliness and the like, and has good popularization and application value.

The invention provides a method for quantitatively detecting microcystic toxins and a recombinant plasmid, a specific primer is designed aiming at the recombinant plasmid, and the microcystic toxins in a water environment are quantitatively detected by adopting a real-time quantitative PCR (Polymerase Chain Reaction) method. Experiments prove that the method for quantitatively detecting the microcystic toxins and the recombinant patent have the characteristics of combination of broad-spectrum recognition and specificity, are high in sensitivity, simple and convenient in preparation method, capable of being stored for a long time, good in purity and wide in linear detection range, and can be used for rapid quantitative detection of the microcystic toxins in water environment samples.

The present invention belongs to the field of environmental and food pollution detection, and relates to a dual-modal detection method for microcystin-LR (MC-LR) based on regulating the activity of MIP-Cu@CDs-like laccases. The present invention uses cupric chloride dihydrate, citric acid and ethylenediamine as synthetic substrates, synthesizes fluorescent nanozymes with laccase-like activity in one step by a hydrothermal method, and encapsulates them through a molecular imprinting method, so that they can specifically identify MC-LR and regulate the activity of MIP-Cu@CDs-like laccases, thereby establishing a colorimetric and fluorescence dual-modal rapid determination method for MC-LR. The method of the present invention has a short detection time, high sensitivity and low cost. The dual-modality provides a built-in cross-reference correction, which greatly improves the reliability and accuracy of MC-LR detection in complex environmental samples and avoids false positive output of the test results.

The invention discloses an ecological risk assessment method for an organophosphorus flame retardant based on multi-dimensional toxicity indexes. The ecological risk assessment method comprises the following steps: culturing microcystis aeruginosa, assessing physiological and biochemical influence, assessing algal toxin synthesis and release influence, assessing a combined toxicity action mode of TCPP and microcystin, and assessing the influence of TCPP on the ecological risk of a water body. According to the method disclosed by the invention, the growth and metabolism change of microcystis aeruginosa and the combined toxicity and ecological risk of algal toxin and the organophosphorus flame retardant under the action of different concentrations of the organophosphorus flame retardant in the water body are evaluated, and the evaluation method gives consideration to the macroscopic and microscopic processes of microalgae in the water body, so that the ecological risk of the organophosphorus flame retardant in the water body can be systematically and comprehensively evaluated; the evaluation method can provide powerful theoretical basis and support for behavior research and use management of the organophosphorus flame retardant in the water environment.

The present application relates to the technical field of human microcystin-1, and specifically to monoclonal antibodies binding to human microcystin-1 and their applications. The embodiment successfully developed a rabbit monoclonal antibody with high affinity against human microcystin-1 through single B cell screening and culture technology, which has high affinity and specificity for human microcystin-1, and the monoclonal antibody can be used to prepare immunoblotting kits and immune combination kits for detecting human microcystin-1. The monoclonal antibody also has important clinical value for the diagnosis and treatment of human microcystin-1 and related diseases.

A water body algae toxin concentration estimation method and system based on remote sensing image belong to the technical field of inland water body eutrophication lake water environment evaluation, solve the technical problems that the existing technology has high precision requirement of detection instrument, expensive equipment material, complex and low efficiency of water sample pretreatment process, and the remote sensing indirect inversion method has poor accuracy and is difficult to realize accurate quantification of microcystin. Step 1, obtain the remote sensing image of the water body, and pretreat to obtain the remote sensing reflectivity data; step 2, calculate the spectral index based on the remote sensing reflectivity data; step 3, construct a water body microcystin concentration remote sensing estimation model, take the spectral index as the input of the water body microcystin concentration remote sensing estimation model, and obtain the water body microcystin concentration. The present application is used for realizing efficient and accurate water body algae toxin concentration estimation.

The application discloses a Pseudomonas capable of degrading microcystins, an immobilized bacterial agent and application, and particularly relates to Pseudomonas chengduensis DMC-X1, which is isolated and screened from surface water samples of a cyanobacterial bloom outbreak in Nanyi Reservoir in Nanjing County, Zhangzhou City, Fujian Province. The strain belongs to gamma-proteobacteria, has high degradation capacity for microcystins (MCs), and has a degradation capacity superior to most Pseudomonas reported at present, and can be used for treating water polluted by microcystins. In the application, sodium alginate is used as a carrier, CaCl2 is used as a crosslinking agent, and the Pseudomonas DMC-X1 is embedded to obtain an immobilized bacterial agent, which can degrade microcystins in a short time and can be recycled for multiple times. The immobilized bacterial agent is convenient to store and recycle, and has important application value.

The present invention discloses a strain of Stenotrophomonas geniculata that can degrade microcystins, an immobilized bacterial agent, and applications. Specifically, the strain is Stenotrophomonas geniculata DMC-X3, which was isolated and screened from a surface water sample of a cyanobacterial bloom outbreak in Nanyi Reservoir, Nanjing County, Zhangzhou City, Fujian Province. The strain DMC-X3 has good degradation performance for both low and high concentrations of MC-LR, and the degradation rate gradually increases with the increase in the inoculation amount of DMC-X3. At the same time, DMC-X3 can efficiently degrade microcystins under environmental pressures of extreme pH and temperature. The present invention also uses sodium alginate and calcium chloride as immobilization materials to immobilize DMC-X3, and the obtained immobilized bacterial agent can repeatedly and efficiently degrade microcystins.