35 results about "Microcystin" patented technology

The invention provides a preparation method and application of a high-sensitivity imprinted membrane for selectively detecting microcystic toxins (MC-LR) in water based on surface enhanced Raman scattering. The preparation method comprises the following steps: step 1, preparation of silver nanocubes; step 2, preparation of Ag NCs-coated TiO2 nanoparticles; step 3, preparation of an Ag NCs-coated TiO2 / PDMS composite membrane; according to the invention, a membrane technology, an SERS technology and MIT are combined, so that the prepared product has excellent physical and chemical properties of a PDMS membrane, high sensitivity of an SERS detection technology and high selectivity of MIT; the invention provides a new method for SERS detection and widens the application range.

The invention relates to a bio-based adsorbent capable of removing microcystic toxins-LR in water, a preparation method of the bio-based adsorbent comprises the following steps: performing steam explosion treatment on rice husks, and then jointly treating the steam-exploded rice husks with sodium hydroxide and sodium trimetaphosphate to obtain rice husks containing phosphate ester groups. The prepared bio-based adsorbent can realize adsorption of microcystic toxins-LR in wastewater, and also has efficient adsorption capacity in the presence of humic acid, calcium carbonate, sodium chloride, magnesium sulfate and other organic and inorganic substances. The adsorption rate of the rice hull powder adsorbent subjected to joint modification on microcystic toxins-LR is obviously higher than that of an original rice hull powder adsorbent, and the rice hull powder adsorbent can quickly adsorb pollutants in water and has a good removal effect on the microcystic toxins-LR within the pH range of an algae culture medium; the potential of adsorbing the microcystic toxins-LR in a microalgae culture environment is realized. Meanwhile, as a typical agricultural waste, the rice husks are turned into wealth through a two-step rapid modification method, and a new way for recycling the rice husks is provided. Therefore, the method for removing the microcystic toxins-LR in the water by utilizing the biomass adsorbent is a good choice, so that the pollution removal of the water body and the reutilization of agricultural wastes are realized.

The invention relates to the field of bioanalytical chemistry and environmental monitoring, and discloses a nano fluorescent probe, a preparation method and application thereof, and a method for detecting microcystic toxins in liquid. The nano composite probe comprises an MOF material and nano particles loaded on the MOF material, the nano particles comprise carboxyl-modified up-conversion nano particles and nucleic acid aptamers covalently connected with the up-conversion nano particles through amido bonds, and the up-conversion nano particles contain a thulium element and an erbium element. The nano fluorescent probe has the advantages of high specificity, simplicity and rapidness in operation, high sensitivity, high interference resistance, simple preparation process and low cost, provides an efficient solution for rapid detection of liquid microcystin pollution, and has a remarkable market prospect.

The invention relates to a preparation method of a multi-energy coupling water body shading diaphragm plate and the shading diaphragm plate, and belongs to the field of water environment treatment. The preparation method comprises the following steps: preparing outer-layer functional shell slurry consisting of PVDF (Polyvinylidene Fluoride), Fe3O4 and SiO2 aerogel; preparing middle-layer light absorption layer slurry which is composed of nano WO3, nitrogen defect g-C3N4 and Fe3O4 and is filled with nitrogen to regulate and control the density; preparing inner-layer super-hydrophilic layer slurry formed by modified TiO2 doped with Fe < + > and V2O5; and sequentially coating the three layers of slurry by adopting a blade coating method, pre-drying, and curing and forming in an alkaline solution in a mold with a pit structure. The prepared shading diaphragm plate has a three-layer structure of an outer-layer functional shell, a middle-layer regulation and control and an inner-layer functional core, can synchronously realize efficient shading and algal inhibition, microcystin degradation and magnetic control recovery, has the advantages of wide scene adaptability and long service life, and is suitable for algal bloom treatment and pollution regulation and control of static and flowing water bodies.

The present application relates to the technical field of organic matter detection, and specifically discloses a pretreatment process and application of microcystin in sediments. A pretreatment process for microcystin in sediments, comprising the following steps: drying and grinding the sediment; dispersing the pretreated sediment into a solvent and then adding a modified adsorbent, ultrasonicating, filtering to obtain an extract A and a precipitate; washing the precipitate with acetone, taking out the modified adsorbent, adding it to the solvent, heating for desorption, and filtering to obtain an extract B; mixing the extract A and the extract B to obtain a crude extract; the preparation method of the modified adsorbent is: adding tannic acid and a porous material to a chitosan solution, stirring and mixing, then adding a cross-linking agent, stirring and mixing, to obtain; the crude extract is passed through a column and eluted. Adding a modified adsorbent to this process can increase the extraction amount of MC, which is beneficial to the detection of trace microcystin in sediments and reduces the influence of impurities such as proteins on the detection.

The invention belongs to the technical field of small molecule detection, and relates to a nucleic acid-based algal toxin on-site rapid quantitative detection paper chip device and a detection method thereof. A reaction area of the paper chip device is coated with an aptamer c-DNA and a fluorescent group labeled-DNA, and a reading area of the paper chip device is coated with a capture nucleic acid probe d-DNA; the nucleic acid sequence of the aptamer c-DNA is as shown in SEQ ID NO.1, and the 5'end of the c-DNA is marked with biotin; the nucleic acid sequence of the capture nucleic acid probe is shown as SEQ ID NO.1, the nucleic acid sequence of the capture nucleic acid probe is shown as SEQ ID NO.2, the 5'end of the capture nucleic acid probe is marked with an FAM fluorophore, the nucleic acid sequence of the capture nucleic acid probe d-DNA is shown as SEQ ID NO.3, and the 5 'end of the capture nucleic acid probe d-DNA is marked with biotin. The whole detection process does not exceed 10 minutes, the cost is low, and rapid on-site quantitative detection of algal toxin in water is achieved.

The invention discloses a method for efficiently removing algae based on trace copper ion activated calcium peroxide, and under the conditions that the addition amount of Cu (II) is only 15 mu M (1 mgL <-1 >), the molar ratio of Cu (II) to CaO2 is 1: 7, and the pH is neutral, the removal rate of algae cells with the concentration of 1.0-2.5 * 10 < 6 > cell smmL <-1 > is higher than 97%. According to the system, the circulation of Cu (II) / Cu (I) is promoted by utilizing the alkalinity of CaO2, more active free radicals such as Cu (III), OH, O2 <-> and < 1 > O2 are generated, and the oxidation algae removal capacity of the system is remarkably improved; the direct attack of copper ions on algae cells can be inhibited by the mild oxidizing property of CaO2, and the release risk of soluble algae organic matters and microcystic toxins is reduced; ca (OH) 2 generated by hydrolysis of CaO2 after the reaction can synchronously precipitate algae cells and copper ions in the water body, so that the concentration of residual copper is reduced to 30.1 mu gL <-1 > or below, and the risk of secondary pollution of heavy metals is effectively avoided. The coupling system successfully overcomes the defects that traditional H2O2 is fast in decomposition, narrow in pH application range, inconvenient to transport and the like, has the advantages of being high in algae removal efficiency, high in adaptability, environmentally friendly and the like, and has good application and popularization value.

The present invention discloses a method for extracting microcystin from Anabaena flos-aquae, comprising the following steps: (1) obtaining the Anabaena flos-aquae strain that produces microcystin; (2) crushing the Anabaena flos-aquae strain, adding a methanol solution, extracting microcystin under stirring, then performing centrifugation, collecting the supernatant, and evaporating the supernatant to obtain a crude extract; (3) preliminarily purifying the crude extract using a solid-phase extraction column to obtain a refined extract; (4) further purifying the refined extract using semi-preparative liquid chromatography to obtain a solution containing microcystin, and then evaporating and drying to obtain the microcystin. The method for extracting microcystin from Anabaena flos-aquae of the present invention has the advantages of stable properties of the toxin-producing strain, single toxin-producing component, high toxin content, good repeatability of extraction and purification, etc., and can obtain a microcystin standard product with high purity and high yield.

The invention relates to the technical field of pollution treatment, and particularly discloses a composite bio-enzyme preparation for polluted water treatment and a preparation method of the composite bio-enzyme preparation. The invention relates to a composite biological enzyme preparation for polluted water treatment. The composite biological enzyme preparation comprises the following raw materials in parts by weight: 25-30 parts of an amylolytic enzyme compound, 20-25 parts of a proteolytic enzyme compound, 15-20 parts of lipase, 10-15 parts of cellulose hydrolase, 15-20 parts of flora freeze-dried powder, 10-15 parts of porous calcium alginate microspheres, 5-8 parts of chitosan modified silicon dioxide and 3-5 parts of an enzyme stabilizer. According to the application, the content of total microcystic toxins and the content of refractory organic matters in the polluted water can be obviously reduced, the sewage treatment effect is facilitated, and secondary pollution in the sewage treatment process is reduced.

The invention discloses a method for removing microcystis aeruginosa, which comprises the following steps: (1) adding peracetic acid into a microcystis aeruginosa water body, so that the concentration of the peracetic acid in the water body is 0.1-5.0 mmol / L; and (2) carrying out electrolytic reaction on the water body in the step (1) by adopting an electrochemical oxidation device, activating peracetic acid in the water body, and enabling generated free radicals to act on microcystis aeruginosa cells so as to remove the microcystis aeruginosa. According to the method, pseudomonas aeruginosa can be rapidly removed, the degradation rate within 30 minutes exceeds 90%, microcystin-LR is eliminated, and the biotoxicity is remarkably reduced.

The present invention belongs to the technical field of treatment of bloom cyanobacteria, and specifically discloses a recombinant enzyme for degrading microcystin in cyanobacteria slurry and a preparation method thereof. The recombinant enzyme has a solubility-enhancing tag NusA linked to the N-terminus of microcystin-degrading enzyme MlrA and a histidine tag linked to the C-terminus. This recombinant enzyme is characterized by strong stability and high expression level, and can still stably exist and efficiently degrade microcystin in a complex environment with large temperature difference in cyanobacteria accumulation, large pH change, low dissolved oxygen, and high nitrogen and phosphorus. After being treated with the crude extract of the recombinant enzyme for 24 h, the degradation efficiency of microcystin in the accumulated cyanobacteria slurry reaches 89.2%.

The invention discloses a time-resolved fluorescent quantitative detection microcystin test strip as well as a preparation method and a detection method of the time-resolved fluorescent quantitative detection microcystin test strip. The test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are arranged on a bottom plate. A combination pad of the test strip is coated with a europium-labeled microcystin nucleic acid aptamer nanoprobe, and a nitrocellulose membrane is provided with a detection line and a quality control line. During detection, microcystic toxins in a sample are competitively combined with the probe to influence a T line fluorescence signal, and accurate quantification can be realized by measuring the fluorescence intensity ratio of a T / C line and substituting the fluorescence intensity ratio into a standard curve. The high specificity of the nucleic acid aptamer and the high sensitivity of time-resolved fluorescence are combined, and the probe structure, the reagent formula and the preparation process are optimized, so that the detection has the advantages of high sensitivity, strong specificity, rapidness, simplicity and convenience in operation, good stability and the like, and is suitable for on-site rapid detection of microcystin in a water environment and an algae sample.

The invention discloses a nano-microsphere time-resolved fluorescent probe as well as a preparation method and application thereof. The preparation method comprises the following three steps: preparation of core-layer nano microspheres, shell-layer functional modification and fluorescence enhancement and stabilization treatment. A polylactic acid-glycolic acid copolymer (PLGA) is used as a core-layer carrier to load a rare earth ion complex, and a SiO2 coating layer and a targeting ligand are combined for modification, so that the nano-microsphere fluorescent probe with a core-shell structure is prepared. The probe has the characteristics of long fluorescence lifetime (5-8ms), high quantum yield (greater than or equal to 35%), strong stability, excellent targeting property and the like, and can be widely applied to food safety detection (such as quinolone antibiotics and microcystin MC-LR) and environmental pollution detection (such as heavy metal ions such as Hg < 2 + > and Pb < 2 + >), and the detection limit is as low as 0.05 ng / mL or 0.1 nmol / L. The problems that a traditional fluorescent probe is low in loading capacity, poor in stability and insufficient in targeting property are solved, and the method has remarkable industrial application value.

The invention discloses a microcystic toxin degrading enzyme MlrA mutant and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The microcystic toxin degrading enzyme MlrA mutant is obtained by carrying out single-point mutation on the 179th or 284th amino acid residue of the amino acid sequence of the wild microcystic toxin degrading enzyme MlrA as shown in SEQ ID No.1; the single point mutation is that phenylalanine at the 179th site is mutated into tyrosine, and arginine at the 284th site is mutated into glutamic acid. Two genetically engineered bacteria for expressing the mutant microcystic toxin degrading enzyme MlrA are obtained through a genetic engineering technology, MC-LR is degraded by utilizing the genetically engineered bacteria, the service life of cells is greatly prolonged, and the requirements of current industrial application are met.

The application discloses a method for efficiently removing algae based on activation of calcium peroxide by trace copper ions, and under the conditions that the Cu(II) dosage is only 15 micromoles, the Cu(II) / CaO2 molar ratio is 1:7, and the pH is neutral, the removal rate of algae cells is higher than 97% for the algae with a concentration of 1.0-2.5*10 6 cells per milliliter. ‑1 The removal rate of algae cells is higher than 97%. The system utilizes the alkalinity of CaO2 to promote the cycle of Cu(II) / Cu(I), generates more Cu(III) and active free radicals such as ∙OH, ∙O2 ‑ 、 1 O2, significantly improves the oxidation and algae removal ability of the system; the mild oxidizing property of CaO2 can inhibit the direct attack of copper ions on algae cells, and reduces the release risk of dissolved algae organic matter and microcystin; after the reaction, Ca(OH)2 generated by the hydrolysis of CaO2 can simultaneously precipitate the algae cells and copper ions in the water body, so that the residual copper concentration is reduced to 30.1 micromoles per liter. ‑1 The following effectively avoids the risk of secondary pollution of heavy metals. That is, the coupling system successfully overcomes the defects of traditional H2O2, such as fast decomposition, narrow pH application range, inconvenient transportation and the like, and has the characteristics of high algae removal efficiency, strong adaptability, environmental friendliness and the like, and has good popularization and application value.

A water body algae toxin concentration estimation method and system based on remote sensing image belong to the technical field of inland water body eutrophication lake water environment evaluation, solve the technical problems that the existing technology has high precision requirement of detection instrument, expensive equipment material, complex and low efficiency of water sample pretreatment process, and the remote sensing indirect inversion method has poor accuracy and is difficult to realize accurate quantification of microcystin. Step 1, obtain the remote sensing image of the water body, and pretreat to obtain the remote sensing reflectivity data; step 2, calculate the spectral index based on the remote sensing reflectivity data; step 3, construct a water body microcystin concentration remote sensing estimation model, take the spectral index as the input of the water body microcystin concentration remote sensing estimation model, and obtain the water body microcystin concentration. The present application is used for realizing efficient and accurate water body algae toxin concentration estimation.

The application discloses a Pseudomonas capable of degrading microcystins, an immobilized bacterial agent and application, and particularly relates to Pseudomonas chengduensis DMC-X1, which is isolated and screened from surface water samples of a cyanobacterial bloom outbreak in Nanyi Reservoir in Nanjing County, Zhangzhou City, Fujian Province. The strain belongs to gamma-proteobacteria, has high degradation capacity for microcystins (MCs), and has a degradation capacity superior to most Pseudomonas reported at present, and can be used for treating water polluted by microcystins. In the application, sodium alginate is used as a carrier, CaCl2 is used as a crosslinking agent, and the Pseudomonas DMC-X1 is embedded to obtain an immobilized bacterial agent, which can degrade microcystins in a short time and can be recycled for multiple times. The immobilized bacterial agent is convenient to store and recycle, and has important application value.

The present invention discloses a strain of Stenotrophomonas geniculata that can degrade microcystins, an immobilized bacterial agent, and applications. Specifically, the strain is Stenotrophomonas geniculata DMC-X3, which was isolated and screened from a surface water sample of a cyanobacterial bloom outbreak in Nanyi Reservoir, Nanjing County, Zhangzhou City, Fujian Province. The strain DMC-X3 has good degradation performance for both low and high concentrations of MC-LR, and the degradation rate gradually increases with the increase in the inoculation amount of DMC-X3. At the same time, DMC-X3 can efficiently degrade microcystins under environmental pressures of extreme pH and temperature. The present invention also uses sodium alginate and calcium chloride as immobilization materials to immobilize DMC-X3, and the obtained immobilized bacterial agent can repeatedly and efficiently degrade microcystins.

The invention discloses a method for extracting and detecting microcystic toxins in water, and belongs to the technical field of water quality detection.The method comprises the steps that cyclodextrin and a compound with a quaternary ammonium salt structure are polymerized, and a porous cyclodextrin polymer with cations is obtained and serves as a cation modified solid-phase extraction column; the covalently-bound and non-covalently-bound microcystic toxins are converted into free microcystic toxins through enzymolysis and the cation modified solid-phase extraction column, the recovery rate is high, and the detection accuracy is improved.

The invention relates to an aggregation-induced electrochemical luminescence microcystic toxin-LR sensing platform based on a supramolecular organic framework, and provides a preparation and detection method of the platform. The invention particularly relates to preparation of the aggregation-induced electrochemiluminescence microcystic toxin-LR sensing platform based on the supramolecular organic framework and a detection method of the aggregation-induced electrochemiluminescence microcystic toxin-LR sensing platform based on the supramolecular organic framework. A subject-object recognition and supramolecular constraint enhanced AIECL strategy is adopted, and CB [8] is selected as a host molecule. The molecular movement of TPE-CEPy is limited through the host-guest binding effect, and a TPE-CEPy-CB [8] composite system is successfully constructed. The material is constructed based on a subject-object recognition mechanism, and the luminous efficiency and stability of the AIECL are remarkably improved. The invention belongs to the field of analytical chemistry.

The invention discloses a feed additive composition for relieving microcystin toxicity and preparation and application thereof.The composition is composed of eucommia ulmoides extract, astragalus membranaceus extract, radix bupleuri extract and macleaya cordata extract.The plant extract composition is added into feed, so that the growth performance of aquatic organisms can be promoted, the oxidation resistance and immunity are enhanced, and the microcystin toxicity can be relieved. The survival rate under the stress of the microcystic toxins is improved. The raw materials are non-medication parts of Chinese herbal medicines, and are low in price, ecological, environment-friendly, healthy and pollution-free.

The invention provides a preparation method of an organic photoelectrochemical transistor sensing platform for detecting microcystic toxins, which comprises the following steps: preparing a polydopamine nanosphere through a dopamine hydrochloride self-assembly reaction, and carrying out modification of a microcystic toxin second antibody and sealing treatment of bovine serum albumin to obtain the organic photoelectrochemical transistor sensing platform for detecting the microcystic toxins. According to the method, a microcystic toxin detection platform with high sensitivity and good selectivity is constructed by utilizing the specific binding capacity of the polydopamine nanospheres and the high sensitivity characteristic of the organic photoelectrochemical transistor; a new technical means is provided for the fields of environment monitoring, food detection and the like, the prepared organic photoelectrochemical transistor sensing platform has good selectivity and stability on microcystic toxins, and the detection limit is 18 pg / mL.

The invention relates to an SERS (Surface Enhanced Raman Scattering) imprinted membrane constructed on the basis of a photo-initiation technology, preparation of the SERS imprinted membrane and application of the SERS imprinted membrane to selective detection of microcystic toxins in water. The preparation method comprises the following steps: step 1, preparation of silver nanocubes; step 2, preparation of Ag NCs-coated Cu2O nanoparticles; step 3, preparation of an Ag NCs-coated Cu2O / PDMS (Polydimethylsiloxane) composite membrane; according to the invention, a membrane technology, an SERS technology and MIT are combined, so that the prepared product has excellent physical and chemical properties of a PDMS membrane, high sensitivity of an SERS detection technology and high selectivity of MIT; the invention provides a new method for SERS detection and widens the application range.

The utility model relates to the technical field of sample freezing, and discloses a sample freezing device for analyzing the content of microcystic toxins in algae, which is characterized in that a refrigerator is arranged on a fixed frame, and a sealing machine cover is arranged at the top of the refrigerator. A sample frame is arranged below the cover, and the fixing frame is further provided with a driving mechanism to achieve automatic lifting of the cover. The driving mechanism comprises a lifting motor, the motor is connected with a screw through a coupler, a nut seat and a lifting frame are arranged outside the screw, and overhanging rods are arranged on the two sides of the lifting frame and connected with the machine cover. Through the arrangement of the driving mechanism, the automatic lifting of the machine cover is realized, the manual operation is reduced, the working efficiency is improved, the safety risk caused by the manual operation is avoided, and the labor intensity of operators is reduced. The sample frame is internally provided with a plurality of sample grooves and through holes, so that the sample can be uniformly cooled in the freezing process, and the uniformity of sample freezing and the accuracy of an analysis result are improved.

The invention discloses a water algae detection device based on a GaN chip and a detection method for water algae thereof.The device is formed by integrating a microcystin sensor, a chlorophyll sensor and an ammonium ion sensor which share a substrate layer and a cover plate, and the structure of each unit sequentially comprises a gallium nitride layer, an aluminum gallium nitrogen barrier layer and a gallium nitride cap layer from bottom to top; a source electrode, a drain electrode and a grid electrode region are arranged on the gallium nitride cap layer in parallel, a sensing film with a microcystin and chlorophyll a combination function and an organic molecular polymer serving as an ammonium ion sensing film are deposited on the surface of the grid electrode region of each unit, and the aluminum gallium nitrogen barrier layer and the gallium nitride layer in the sensor form a heterojunction. Two-dimensional electron gas is formed in the channel and is sensitive to potential change of the surface of the grid electrode, and compared with a traditional water algae detection means, the method is more portable, detection is rapid, and the result is accurate.

The application belongs to the technical field of electrochemical detection and analysis, and particularly relates to a light-assisted enhanced self-powered electrochemical sensor for detecting microcystin-RR (MC-RR) and a preparation method thereof. 18 O 49 A heterojunction is further formed with Nb2C MXene. Polarization of the molecule can be achieved by doping Mo atoms into W 18 O 49 , which promotes electron transfer. The Fermi level of Nb2C MXene is lower, and the conductivity is higher. The application widens the application of W 18 O 49 in self-powered photoelectrochemical sensors, and the prepared sensor has a wide detection range, high sensitivity and low detection limit, and is of great significance for detecting MC-RR.

The invention belongs to the technical field of microorganisms, and particularly relates to a novel sphingosine bacterium as well as strain fermentation liquor and application thereof. The invention discloses a novel sphingosine bacterium which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.28677. The invention further discloses a preparation method of the novel sphingosine bacterium. The novel sphingosine bacterium has efficient degradation capacity on microcystin, and the highest degradation rate is close to 100%. Neosphingosine bacteria and a bacterial strain fermentation broth thereof can be used as a microcystin degradation preparation for repairing a microcystin polluted environment.

The application discloses a microcystin degradation agent in water, a preparation method and application thereof, and utilizes oxalic acid and siderite to prepare a compound oxalic acid degradation agent, the degradation agent can degrade microcystin and antibiotic pollutant cefazolin sodium in water, and the degradation reaction can be carried out in dark light conditions, meanwhile, the reaction pH has a wide range, and the degradation can be carried out at pH 5-9; the degradation agent can degrade more than 93% of the microcystin within 26 hours, and the degradation rate of the cefazolin sodium can reach more than 93% within 10 hours, which indicates that the degradation agent prepared by compounding oxalic acid on the surface of the siderite can be applied to the degradation and removal of microcystin and organic pollutants such as antibiotics in water without external light, and therefore has wide application value.

This invention discloses a method for retrieving microcystin content in water using algal fluorescence spectroscopy. First, fluorescence spectral data of N algal solution samples with different concentrations are measured, and after preprocessing, the data are converted into a one-dimensional array M of length p. p Furthermore, all the corresponding one-dimensional arrays are combined into a matrix X. N The microcystin content of N algal solution samples was determined, forming a matrix Y. N Based on matrix X N With matrix Y N Establish model M ridge The fluorescence spectrum data of the sample to be tested were measured, and after preprocessing, they were converted into a one-dimensional array M of length p. px This is further transformed into matrix X. p Substitute into the established model M ridge In the model, the predicted values ​​of microcystin are automatically provided. Model M ridge During the modeling process, factors affecting fluorescence spectroscopy and algal growth can be incorporated to further improve model accuracy. This method is more accurate, simple, quick, and convenient, facilitating on-site monitoring and possessing significant practical value.