16 results about "Microcystin" patented technology

The invention relates to a preparation method of a multi-energy coupling water body shading diaphragm plate and the shading diaphragm plate, and belongs to the field of water environment treatment. The preparation method comprises the following steps: preparing outer-layer functional shell slurry consisting of PVDF (Polyvinylidene Fluoride), Fe3O4 and SiO2 aerogel; preparing middle-layer light absorption layer slurry which is composed of nano WO3, nitrogen defect g-C3N4 and Fe3O4 and is filled with nitrogen to regulate and control the density; preparing inner-layer super-hydrophilic layer slurry formed by modified TiO2 doped with Fe < + > and V2O5; and sequentially coating the three layers of slurry by adopting a blade coating method, pre-drying, and curing and forming in an alkaline solution in a mold with a pit structure. The prepared shading diaphragm plate has a three-layer structure of an outer-layer functional shell, a middle-layer regulation and control and an inner-layer functional core, can synchronously realize efficient shading and algal inhibition, microcystin degradation and magnetic control recovery, has the advantages of wide scene adaptability and long service life, and is suitable for algal bloom treatment and pollution regulation and control of static and flowing water bodies.

The invention relates to the technical field of pollution treatment, and particularly discloses a composite bio-enzyme preparation for polluted water treatment and a preparation method of the composite bio-enzyme preparation. The invention relates to a composite biological enzyme preparation for polluted water treatment. The composite biological enzyme preparation comprises the following raw materials in parts by weight: 25-30 parts of an amylolytic enzyme compound, 20-25 parts of a proteolytic enzyme compound, 15-20 parts of lipase, 10-15 parts of cellulose hydrolase, 15-20 parts of flora freeze-dried powder, 10-15 parts of porous calcium alginate microspheres, 5-8 parts of chitosan modified silicon dioxide and 3-5 parts of an enzyme stabilizer. According to the application, the content of total microcystic toxins and the content of refractory organic matters in the polluted water can be obviously reduced, the sewage treatment effect is facilitated, and secondary pollution in the sewage treatment process is reduced.

The invention discloses a time-resolved fluorescent quantitative detection microcystin test strip as well as a preparation method and a detection method of the time-resolved fluorescent quantitative detection microcystin test strip. The test strip comprises a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are arranged on a bottom plate. A combination pad of the test strip is coated with a europium-labeled microcystin nucleic acid aptamer nanoprobe, and a nitrocellulose membrane is provided with a detection line and a quality control line. During detection, microcystic toxins in a sample are competitively combined with the probe to influence a T line fluorescence signal, and accurate quantification can be realized by measuring the fluorescence intensity ratio of a T / C line and substituting the fluorescence intensity ratio into a standard curve. The high specificity of the nucleic acid aptamer and the high sensitivity of time-resolved fluorescence are combined, and the probe structure, the reagent formula and the preparation process are optimized, so that the detection has the advantages of high sensitivity, strong specificity, rapidness, simplicity and convenience in operation, good stability and the like, and is suitable for on-site rapid detection of microcystin in a water environment and an algae sample.

The invention discloses a nano-microsphere time-resolved fluorescent probe as well as a preparation method and application thereof. The preparation method comprises the following three steps: preparation of core-layer nano microspheres, shell-layer functional modification and fluorescence enhancement and stabilization treatment. A polylactic acid-glycolic acid copolymer (PLGA) is used as a core-layer carrier to load a rare earth ion complex, and a SiO2 coating layer and a targeting ligand are combined for modification, so that the nano-microsphere fluorescent probe with a core-shell structure is prepared. The probe has the characteristics of long fluorescence lifetime (5-8ms), high quantum yield (greater than or equal to 35%), strong stability, excellent targeting property and the like, and can be widely applied to food safety detection (such as quinolone antibiotics and microcystin MC-LR) and environmental pollution detection (such as heavy metal ions such as Hg < 2 + > and Pb < 2 + >), and the detection limit is as low as 0.05 ng / mL or 0.1 nmol / L. The problems that a traditional fluorescent probe is low in loading capacity, poor in stability and insufficient in targeting property are solved, and the method has remarkable industrial application value.

The application discloses a method for efficiently removing algae based on activation of calcium peroxide by trace copper ions, and under the conditions that the Cu(II) dosage is only 15 micromoles, the Cu(II) / CaO2 molar ratio is 1:7, and the pH is neutral, the removal rate of algae cells is higher than 97% for the algae with a concentration of 1.0-2.5*10 6 cells per milliliter. ‑1 The removal rate of algae cells is higher than 97%. The system utilizes the alkalinity of CaO2 to promote the cycle of Cu(II) / Cu(I), generates more Cu(III) and active free radicals such as ∙OH, ∙O2 ‑ 、 1 O2, significantly improves the oxidation and algae removal ability of the system; the mild oxidizing property of CaO2 can inhibit the direct attack of copper ions on algae cells, and reduces the release risk of dissolved algae organic matter and microcystin; after the reaction, Ca(OH)2 generated by the hydrolysis of CaO2 can simultaneously precipitate the algae cells and copper ions in the water body, so that the residual copper concentration is reduced to 30.1 micromoles per liter. ‑1 The following effectively avoids the risk of secondary pollution of heavy metals. That is, the coupling system successfully overcomes the defects of traditional H2O2, such as fast decomposition, narrow pH application range, inconvenient transportation and the like, and has the characteristics of high algae removal efficiency, strong adaptability, environmental friendliness and the like, and has good popularization and application value.

A water body algae toxin concentration estimation method and system based on remote sensing image belong to the technical field of inland water body eutrophication lake water environment evaluation, solve the technical problems that the existing technology has high precision requirement of detection instrument, expensive equipment material, complex and low efficiency of water sample pretreatment process, and the remote sensing indirect inversion method has poor accuracy and is difficult to realize accurate quantification of microcystin. Step 1, obtain the remote sensing image of the water body, and pretreat to obtain the remote sensing reflectivity data; step 2, calculate the spectral index based on the remote sensing reflectivity data; step 3, construct a water body microcystin concentration remote sensing estimation model, take the spectral index as the input of the water body microcystin concentration remote sensing estimation model, and obtain the water body microcystin concentration. The present application is used for realizing efficient and accurate water body algae toxin concentration estimation.

The invention relates to an aggregation-induced electrochemical luminescence microcystic toxin-LR sensing platform based on a supramolecular organic framework, and provides a preparation and detection method of the platform. The invention particularly relates to preparation of the aggregation-induced electrochemiluminescence microcystic toxin-LR sensing platform based on the supramolecular organic framework and a detection method of the aggregation-induced electrochemiluminescence microcystic toxin-LR sensing platform based on the supramolecular organic framework. A subject-object recognition and supramolecular constraint enhanced AIECL strategy is adopted, and CB [8] is selected as a host molecule. The molecular movement of TPE-CEPy is limited through the host-guest binding effect, and a TPE-CEPy-CB [8] composite system is successfully constructed. The material is constructed based on a subject-object recognition mechanism, and the luminous efficiency and stability of the AIECL are remarkably improved. The invention belongs to the field of analytical chemistry.

The invention relates to an SERS (Surface Enhanced Raman Scattering) imprinted membrane constructed on the basis of a photo-initiation technology, preparation of the SERS imprinted membrane and application of the SERS imprinted membrane to selective detection of microcystic toxins in water. The preparation method comprises the following steps: step 1, preparation of silver nanocubes; step 2, preparation of Ag NCs-coated Cu2O nanoparticles; step 3, preparation of an Ag NCs-coated Cu2O / PDMS (Polydimethylsiloxane) composite membrane; according to the invention, a membrane technology, an SERS technology and MIT are combined, so that the prepared product has excellent physical and chemical properties of a PDMS membrane, high sensitivity of an SERS detection technology and high selectivity of MIT; the invention provides a new method for SERS detection and widens the application range.

The invention discloses a water algae detection device based on a GaN chip and a detection method for water algae thereof.The device is formed by integrating a microcystin sensor, a chlorophyll sensor and an ammonium ion sensor which share a substrate layer and a cover plate, and the structure of each unit sequentially comprises a gallium nitride layer, an aluminum gallium nitrogen barrier layer and a gallium nitride cap layer from bottom to top; a source electrode, a drain electrode and a grid electrode region are arranged on the gallium nitride cap layer in parallel, a sensing film with a microcystin and chlorophyll a combination function and an organic molecular polymer serving as an ammonium ion sensing film are deposited on the surface of the grid electrode region of each unit, and the aluminum gallium nitrogen barrier layer and the gallium nitride layer in the sensor form a heterojunction. Two-dimensional electron gas is formed in the channel and is sensitive to potential change of the surface of the grid electrode, and compared with a traditional water algae detection means, the method is more portable, detection is rapid, and the result is accurate.

The invention belongs to the technical field of microorganisms, and particularly relates to a novel sphingosine bacterium as well as strain fermentation liquor and application thereof. The invention discloses a novel sphingosine bacterium which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.28677. The invention further discloses a preparation method of the novel sphingosine bacterium. The novel sphingosine bacterium has efficient degradation capacity on microcystin, and the highest degradation rate is close to 100%. Neosphingosine bacteria and a bacterial strain fermentation broth thereof can be used as a microcystin degradation preparation for repairing a microcystin polluted environment.

This invention discloses a method for retrieving microcystin content in water using algal fluorescence spectroscopy. First, fluorescence spectral data of N algal solution samples with different concentrations are measured, and after preprocessing, the data are converted into a one-dimensional array M of length p. p Furthermore, all the corresponding one-dimensional arrays are combined into a matrix X. N The microcystin content of N algal solution samples was determined, forming a matrix Y. N Based on matrix X N With matrix Y N Establish model M ridge The fluorescence spectrum data of the sample to be tested were measured, and after preprocessing, they were converted into a one-dimensional array M of length p. px This is further transformed into matrix X. p Substitute into the established model M ridge In the model, the predicted values ​​of microcystin are automatically provided. Model M ridge During the modeling process, factors affecting fluorescence spectroscopy and algal growth can be incorporated to further improve model accuracy. This method is more accurate, simple, quick, and convenient, facilitating on-site monitoring and possessing significant practical value.

This invention discloses a method for establishing Alzheimer's disease in tree shrews. The specific steps involve mixing an ethanol solution of 0.5 μg / μl Microcystin-LR with a muscarine solution, and then injecting 2 μl into each of the bilateral Meynert basal ganglia of the tree shrew. After administration, the tree shrew is fed normally for two weeks to complete the Alzheimer's disease model. The muscarine solution is prepared under sterile conditions by adding 200 μl of sterile saline to a vial containing 1 mg of muscarine, repeatedly pipetting to ensure complete dissolution, resulting in a concentration of 5 μg / μl. The Microcystin-LR solution is a commercially available product. This invention involves injecting a mixture of 0.1 μg MC-LR and 3 μg IBO solution into the bilateral Meynert basal ganglia of tree shrews, establishing a stable tree shrew AD model after two weeks. Three days after administration, tree shrews exhibited AD behavioral manifestations, such as lethargy and reduced activity. Two weeks later, the tree shrew brains showed typical AD pathological manifestations, including Aβ deposition and elevated expression levels at multiple phosphorylation sites of Tau protein.

The invention relates to an aptamer for specifically recognizing tetrodotoxin and application of the aptamer, and belongs to the technical field of detection. The cyclic divalent aptamer disclosed by the invention has good affinity and specificity and good thermal stability, is not interfered by impurities such as ion strength and protein in globefish meat and serum during tetrodotoxin detection, and has high conformational stability. Based on the annular bivalent aptamer, the invention further designs an aptamer sensor based on a 3D-DNA walker, the aptamer sensor can specifically detect low-concentration tetrodotoxin, the detection limit is 0.02 ng / mL, the detection time is 1 h, and the detection sensitivity is high. Other marine toxins such as gonyautoxin (GTX), dinophysiotoxin (DTX), sea anemone toxin II (ATX-II), anabaena toxin-a (ATX-a) and microcystin (MC-LR) cannot be detected, and the specificity is good.

The application provides a high-specificity detection microcystin whole column-SERS / fluorescence dual-mode detection platform and a construction method thereof. The platform takes a three-dimensional porous amino silica gel monolithic column as a carrier, solidifies specific recognition probes through electrostatic adsorption, constructs the whole column-SERS / fluorescence dual-mode detection platform through base complementary pairing of cDNA and aptamers on the specific probe of Au@Ag@cDNA / Aptamer-Cy3, and utilizes the Au@Ag core-shell structure to generate SERS 'hot spot' enhancement effect. When target algae toxin molecules exist in the sample, the aptamers are specifically combined with the target substances and separated from the monolithic column, so that the base SERS signal is weakened and the fluorescence signal of the effluent is enhanced, high-sensitivity and high-specificity dual-mode qualitative or quantitative detection of microcystin is realized through the dual signal changes, and the detection sensitivity and accuracy are improved.

The application discloses a Paucibacter psychrotolerans capable of degrading microcystins and application thereof. The Paucibacter psychrotolerans provided by the application is Paucibacter psychrotolerans 2WFC-1, and the preservation number thereof in the China General Microbiological Culture Collection Center is CGMCC No. 24793. The strain 2WFC-1 belongs to a new species of the Paucibacter genus, can degrade microcystins, and is different from other Paucibacter in that it can grow at a temperature ranging from 4 DEG C to 37 DEG C, has great potential for degrading microcystins in a low-temperature water environment, and has important application prospects in water ecological restoration, seasonal water pollution treatment and drinking water purification.