6 results about "Microcystin" patented technology

This invention discloses a special polyferric sulfate suitable for low-temperature, low-turbidity water treatment and its preparation method. The product has a basicity B = 12%–15%, an average degree of polymerization n = 12–15, a Zeta potential ≥ +30mV at 5°C, and contains 0.01–0.05wt% (as Fe₂O₃) of La. 3+ or Ce 3+ A copolymer stabilizer, intercalated between Fe–O–Fe chain segments, was confirmed by XRD to have a lattice distortion rate Δd / d = 0.8%. This material exhibits an adsorption capacity for orthophosphate ≥ 48 mg P / g and a partition coefficient K ≥ 2.4 × 10⁻⁶ for microcystin-LR. 4 L / kg, under conditions of 5–10℃, turbidity 1–5 NTU, pH 6.5–7.2, and a dosage ≤12 mg / L, can achieve a turbidity removal rate ≥90% and UV removal. 254 The removal rate is ≥65%, the TOC removal rate is ≥58%, and the phosphorus removal capacity retention rate is ≥88% after 30 freeze-thaw cycles. The preparation method employs a gradient oxidation process: 25% dilute sulfuric acid (mass fraction), ferrous sulfate to dilute sulfuric acid mass ratio 8.5–9.5:1; 10% sodium nitrite is added dropwise at a uniform rate (0.022–0.033 mg / L). 3 ( / min), the mutation point determination criteria are brownish-red color + divalent iron ≤0.1g / L; filter pore size ≤0.2μm, cooled to 25–35℃ and then stored in a sealed environment at 3–5℃. This invention solves the problems of low activity, poor stability and weak removal of multiple pollutants in low-temperature and low-turbidity scenarios such as cold lakes and black and odorous river sediments, and provides a safe, long-lasting and controllable new functional material for ecological restoration.

A water body algae toxin concentration estimation method and system based on remote sensing image belong to the technical field of inland water body eutrophication lake water environment evaluation, solve the technical problems that the existing technology has high precision requirement of detection instrument, expensive equipment material, complex and low efficiency of water sample pretreatment process, and the remote sensing indirect inversion method has poor accuracy and is difficult to realize accurate quantification of microcystin. Step 1, obtain the remote sensing image of the water body, and pretreat to obtain the remote sensing reflectivity data; step 2, calculate the spectral index based on the remote sensing reflectivity data; step 3, construct a water body microcystin concentration remote sensing estimation model, take the spectral index as the input of the water body microcystin concentration remote sensing estimation model, and obtain the water body microcystin concentration. The present application is used for realizing efficient and accurate water body algae toxin concentration estimation.

This invention discloses a method for retrieving microcystin content in water using algal fluorescence spectroscopy. First, fluorescence spectral data of N algal solution samples with different concentrations are measured, and after preprocessing, the data are converted into a one-dimensional array M of length p. p Furthermore, all the corresponding one-dimensional arrays are combined into a matrix X. N The microcystin content of N algal solution samples was determined, forming a matrix Y. N Based on matrix X N With matrix Y N Establish model M ridge The fluorescence spectrum data of the sample to be tested were measured, and after preprocessing, they were converted into a one-dimensional array M of length p. px This is further transformed into matrix X. p Substitute into the established model M ridge In the model, the predicted values ​​of microcystin are automatically provided. Model M ridge During the modeling process, factors affecting fluorescence spectroscopy and algal growth can be incorporated to further improve model accuracy. This method is more accurate, simple, quick, and convenient, facilitating on-site monitoring and possessing significant practical value.

This invention discloses a method for establishing Alzheimer's disease in tree shrews. The specific steps involve mixing an ethanol solution of 0.5 μg / μl Microcystin-LR with a muscarine solution, and then injecting 2 μl into each of the bilateral Meynert basal ganglia of the tree shrew. After administration, the tree shrew is fed normally for two weeks to complete the Alzheimer's disease model. The muscarine solution is prepared under sterile conditions by adding 200 μl of sterile saline to a vial containing 1 mg of muscarine, repeatedly pipetting to ensure complete dissolution, resulting in a concentration of 5 μg / μl. The Microcystin-LR solution is a commercially available product. This invention involves injecting a mixture of 0.1 μg MC-LR and 3 μg IBO solution into the bilateral Meynert basal ganglia of tree shrews, establishing a stable tree shrew AD model after two weeks. Three days after administration, tree shrews exhibited AD behavioral manifestations, such as lethargy and reduced activity. Two weeks later, the tree shrew brains showed typical AD pathological manifestations, including Aβ deposition and elevated expression levels at multiple phosphorylation sites of Tau protein.

The application provides a high-specificity detection microcystin whole column-SERS / fluorescence dual-mode detection platform and a construction method thereof. The platform takes a three-dimensional porous amino silica gel monolithic column as a carrier, solidifies specific recognition probes through electrostatic adsorption, constructs the whole column-SERS / fluorescence dual-mode detection platform through base complementary pairing of cDNA and aptamers on the specific probe of Au@Ag@cDNA / Aptamer-Cy3, and utilizes the Au@Ag core-shell structure to generate SERS 'hot spot' enhancement effect. When target algae toxin molecules exist in the sample, the aptamers are specifically combined with the target substances and separated from the monolithic column, so that the base SERS signal is weakened and the fluorescence signal of the effluent is enhanced, high-sensitivity and high-specificity dual-mode qualitative or quantitative detection of microcystin is realized through the dual signal changes, and the detection sensitivity and accuracy are improved.

The application discloses a Paucibacter psychrotolerans capable of degrading microcystins and application thereof. The Paucibacter psychrotolerans provided by the application is Paucibacter psychrotolerans 2WFC-1, and the preservation number thereof in the China General Microbiological Culture Collection Center is CGMCC No. 24793. The strain 2WFC-1 belongs to a new species of the Paucibacter genus, can degrade microcystins, and is different from other Paucibacter in that it can grow at a temperature ranging from 4 DEG C to 37 DEG C, has great potential for degrading microcystins in a low-temperature water environment, and has important application prospects in water ecological restoration, seasonal water pollution treatment and drinking water purification.