Recyclable fluorescent sensor for detecting microcystic toxins and application method of fluorescent sensor

Abstract

The invention discloses a recyclable fluorescent sensor for detecting microcystic toxins and an application method of the fluorescent sensor, and belongs to the field of analytical chemistry. The application method comprises the steps that the surfaces of two groups of graphene quantum dots are modified with probe DNA1 and probe DNA2 which achieve base complementation with nucleic acid aptamer DNA corresponding to the microcystic toxins respectively, the nucleic acid aptamer DNA corresponding to the microcystic toxins is added, the graphene quantum dots are made to be aggregated through DNA hybridization to generate exciton energy transfer, and then fluorescent signals of the graphene quantum dots are quenched; the microcystic toxins are added and specially combined with the nucleic acid aptamer DNA, the structure is transformed to cause graphene quantum dot aggregation to be disassembled and dispersed again, and the fluorescent intensity of the system is restored. The method is simple in process, easy to operate, green, environmentally friendly, low in cost, high in sensitivity, good in specificity and excellent in cycle performance and is successfully applied in added-standard recovery of the microcystic toxins in actual tap water and an Olympic Park lake water sample.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and in particular relates to a recyclable method for detecting microcystins by utilizing a nucleic acid aptamer structure conversion strategy to regulate the aggregation state of graphene quantum dots, thereby regulating the quenching and recovery of fluorescent signals. Background technique [0002] Cyanobacterial bloom pollution caused by eutrophication of water bodies is one of the water environment problems that are generally concerned at home and abroad. Cyanobacterial bloom is a manifestation of water eutrophication. It refers to the phenomenon of rapid and massive reproduction or aggregation of cyanobacteria under suitable light, temperature, climate, hydrology and other conditions when the water body is in an eutrophic state. [0003] Microcystins (MCs) are highly toxic cyanobacterial toxins produced in the process of cyanobacterial blooms, which are caused by excessive nitrogen and ph...

AI Technical Summary

Problems solved by technology

The above detection methods require a certain operation process such as complex detection sample pretreatment, and at the same time it takes a long time to detect and analyze microcystin samples, and there are problems such as the complicated and expensive experimental equipment used for detection.

However, the method of constructing fluorescent sensors based on nucleic acid aptamers is limited by the poor photostability of traditional fluorescent dye groups.

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