222 results about "Cyanobacteria" patented technology

The invention discloses a plug flow type mechanical pressurization cyanobacteria control ship, which belongs to the field of ecological environment governance, and mainly comprises a ship body, an algae collection head, a buoyancy cabin, a water collection tank, a pressurization treatment cabin and a pressurization assembly, the lever principle and the electric push rod are used for pressurizing, so that compared with a mode of directly pressurizing a water body by using an air compressor, a water pump and the like, the treatment energy consumption is low, and the pressurizing speed is higher; through the combined design of the dustpan-shaped algae collecting head, the algae inlet position, the pressurization treatment bin layering and the like, the entering and the discharging of high-algae-content water are realized by utilizing the ship advancing power and the water impeller, the operation energy consumption is greatly reduced compared with the water pump sampling, and the device can be suitable for blue-green algae prevention and control in water areas such as lakes, reservoirs, water sources, landscape water bodies and the like.

The present invention discloses a method for extracting a stable body from cyanobacteria, comprising the following steps: S1. Cultivating cyanobacteria; S2. Pretreating cyanobacteria; S3. Ultrasonic disruption; S4. Differential centrifugation; S5. Ultrafiltration; S6. Ultracentrifugation; S7. Density gradient centrifugation; S8. Removing peripheral proteins. The extraction method provided by the present invention has high extraction efficiency, stable and controllable process, no toxic by-products, is green and environmentally friendly, and does not require high temperature and high pressure equipment and large instruments and equipment, thereby expanding application scenarios and reducing application costs. The present invention can obtain high polyP n Stable, active granular biomaterials with high polymerization degree, concentration, and purity, as well as high bioactivity, can meet the stringent requirements of applications in the food and daily chemical sectors, as well as clinical medicine and scientific research. They possess significant practical application value and commercial prospects, and will address the technical challenges of preparing stable, active granular biomaterials in China.

The invention discloses a lake cyanobacterial bloom intelligent early warning method based on unmanned aerial vehicle remote sensing and image recognition, and the method comprises the steps: fusing an unmanned aerial vehicle multispectral image and a convolutional neural network, extracting the spectral features of cyanobacteria, and calculating the concentration distribution; image registration and a dynamic model are combined to track water bloom boundary change and drift trajectory, satellite remote sensing is used to verify precision and invert biomass density, early warning levels are divided according to the precision and the biomass density, decision information is generated, and intelligent monitoring and accurate early warning of cyanobacterial bloom are realized. The comprehensive technical effects of water bloom dynamic monitoring, accurate early warning and efficient management are achieved, and a scientific basis is provided for water environment treatment.

The invention belongs to the technical field of lake and reservoir water source risk prediction, and provides a lake and reservoir water source algae bloom risk early warning system based on a 16S / 18SrRNA gene expression quantity threshold, and the method comprises the following steps: S1, collecting a sample from a lake and reservoir water source surface layer water body; s2, using 7-gate water bloom algae specific primers for blue-green algae, green algae, diatom, euglena, dinoflagellate, chrysophyta and cryptoalga; s3, based on the qPCR standard curve, calculating the copy number of the 16SrRNA / 18SrRNA gene of the seven water bloom algae; s4, when the gene expression quantity of a certain algal bloom algae continuously reaches 106-7 copy number / mL for 7-12 days, determining that the algal bloom algae is in a window phase; according to the threshold value of the gene expression quantity of the water bloom algae 16SrRNA / 18SrRNA, the window period of water bloom algae cells can be accurately recognized, so that early warning of the algae bloom risk in the lake and reservoir water source is achieved, and early warning of algae bloom outbreak in the lake and reservoir water source is achieved one week or above in advance.

The invention relates to the technical field of blue-green algae cleaning, and discloses a blue-green algae cleaning boat and a cleaning method.The blue-green algae cleaning boat comprises a boat body, a bearing frame, a cleaning assembly, a dumping and collecting box and a power supply driving assembly, and the boat is composed of two suspension bodies to improve buoyancy and stability. The bearing frame is located on the inner side of the ship body and provides a bearing area for installing the cleaning assembly and other related components. The cleaning assembly is located above the bearing frame and used for cleaning and conveying algae pollutants. And the dumping and collecting box is arranged at the tail end of the cleaning assembly and is used for receiving and storing the cleaned and conveyed algae pollutants. The power supply driving assembly provides assembly working power and supports work of the cleaning assembly. The small boat firstly runs to a preset position and is accurately positioned by additionally arranging a built-in navigation system. And after the blue-green algae arrives at the target area, the cleaning assembly starts to work, and the blue-green algae is removed from the water surface through a cutting and conveying mechanism and conveyed into a dumping and collecting box. The cleaned algae are directly conveyed into the collecting box through the conveying device, and the pollutant collecting process is completed.

The invention provides a blue-green algae image recognition method and system based on hierarchical self-adaption and domain driving, and the method comprises the steps: enhancing an image through employing an improved dark channel algorithm; constructing a blue-green algae biological attribute text database, and performing synonym replacement and sentence pattern recombination; multi-scale visual features are extracted through a hierarchical adaptive Swin Transform model, and key region characterization is enhanced in combination with dynamic spectrum attention; the text is input into a Bio-ALBERT model, and semantic embedding of field optimization is generated through term mask prediction and attribute relation pre-training; constructing a two-layer heterogeneous graph by using a graph attention interaction network GAIN, calculating a cross-modal association weight through a bidirectional graph attention mechanism, and outputting a cross-modal graph feature; multi-scale cross-modal association is modeled through a hierarchical graph attention fusion mechanism, and a comparison alignment loss optimization model is combined; and high-precision blue-green algae identification is realized. According to the method, through multi-scale perception, domain semantic adaptation and graph structure fusion, the accuracy of blue-green algae detection in a complex environment is improved.

The invention discloses a blue-green algae identification and quantification method and device based on unmanned aerial vehicle remote sensing images and deep learning and a medium, and relates to the technical field of information data processing. Combining meteorological data and water quality monitoring data to construct an adaptive dynamic environment algorithm to extract EXIF metadata including camera parameters and attitude angle information, and establishing a geometric projection model to perform coarse orthographic correction on an original aerial image; the method comprises the following steps: extracting a multi-scale cyanobacterial bloom image feature map by stages based on a ResNet architecture and in combination with a feature pyramid network FPN fused with an attention mechanism, and obtaining an instance mask of cyanobacterial bloom through an anchor-free region proposal network, ROI Align and a head network; based on an improved GIS space projection and deep learning algorithm, carrying out high-precision area measurement and calculation on a binary mask image converted from the instance mask; according to the method, the influence of irrelevant interference on blue-green algae identification can be reduced, and the accuracy and comparability of blue-green algae identification and area calculation are improved.

The invention discloses a confocal laser microscope detection method for micro-plastic surface biofilm components and biomass, and belongs to the field of micro-plastic pollution monitoring. The method comprises the following steps: carrying out stratified sampling and polymer component identification on micro-plastic in an environmental water body; the method comprises the following steps: marking algae, blue-green algae, bacteria and extracellular polymeric substances in a biological membrane through specific fluorescent staining, carrying out high-resolution three-dimensional imaging by utilizing a confocal laser microscope, and realizing batch quantitative analysis of each component of the biological membrane by adopting ImageJ software. The technical problems that an existing detection method is insufficient in parameter dimension, evaluation result deviation is caused only based on partial component quantification, and the influence of the overall structure of the biological membrane on the micro-plastic migration behavior cannot be truly reflected are solved. According to the method, the biomass and spatial distribution of each component of the biological membrane can be comprehensively and accurately quantified, the accuracy of analyzing the vertical migration mechanism of the micro-plastic is improved, and multi-dimensional data support is provided for ecological risk assessment.

The invention discloses a desert blue-green algae large-scale culture nutrient solution and application thereof. The desert blue-green algae large-scale culture nutrient solution is prepared from urea, monopotassium phosphate, potassium chloride, sodium bicarbonate and medium trace element fertilizer. Wherein the concentrations of the urea, the monopotassium phosphate, the potassium chloride, the sodium bicarbonate and the medium trace element fertilizer are 500-550 mg / L, 20-30 mg / L, 10-15 mg / L, 10-15 mg / L and 10 mg / L respectively. The invention provides a nutrient solution special for large-scale cultivation of desert blue-green algae, and solves the technical problems of high cost, low yield and low blue-green algae purity of large-scale cultivation of desert blue-green algae in the prior art. The nutrient solution composition provided by the invention can balance each nutrient composition, so that the nutrient composition can be more suitable for the nutritional requirements of the growth of the desert blue-green algae.

The present invention is related to the fields of bioplastics. In particular, a strain of photosynthetic cyanobacteria (i.e. blue-green algae) having a high growth rate was engineered to overexpress limonene synthase. These engineered cyanobacteria improve auto-sedimentation for biomass harvest and provide for plastic bioremediation capture. Further, machine-learning methods are provided for improving some of the aspects of cultivating cyanobacteria including, but not limited to, assessing real-time light availability and predicting real-time algae growth rates to increase a cyanobacteria biomass.

A living paint comprises a living photosynthetic microorganism, binder, growth medium, and water. The microorganism may comprise algae, cyanobacteria, or lichens. The binder may be composed of primary

The invention relates to the technical field of water pollution and water body odor substance detection, in particular to a primer group, a kit and a method for detecting cyanobacteria generating 2-methylisoborneol in a water body based on a recombinase-mediated isothermal nucleic acid amplification (RAA) technology. The primer group is designed based on a mic gene conserved region of the 2-methylisoborneol-producing cyanobacteria, and comprises a forward primer, a reverse primer and a specific probe; the 5'end of the reverse primer is modified with a fluorescent marker, the 5 'end of the probe is modified with biotin, the 3' end of the probe is modified with a phosphorylation group, and nuclease cleavage sites are contained in the probe. The primer group disclosed by the invention can effectively amplify the mic gene of the 2-methylisoborneol, has relatively high specificity and sensitivity, has no cross reaction with cyanobacteria which does not generate the 2-methylisoborneol, and can be combined with lateral flow immunochromatography to be used for on-site rapid detection of the 2-methylisoborneol; the method is of great significance in early warning of excessive content of 2-methylisoborneol in the water body.

Disclosed are floating antialgal-agent-loaded sodium alginate hydrogel spheres, a preparation method therefor and use thereof. The preparation method includes preparing a solution A and a solution B; dropwise adding the solution A to the solution B to obtain hydrogel spheres; and filtering and washing to obtain the floating antialgal-agent-loaded sodium alginate hydrogel spheres. The hydrogel spheres may float on the water surface and release copper ions in a low dose for a long time, and continuously inhibit the cyanobacteria on the water surface, with little impact on the water body, which may greatly reduce the risk of water pollution caused by copper ion overdosing. Meanwhile, the hydrogel spheres may effectively reduce the application times of the antialgal agent, reduce the labor cost caused by the long-term application of antialgal agent, float on the water surface for easy recovery, and do not cause secondary pollution to the water body.

The invention relates to the technical field of water circulation treatment, and particularly discloses aquaculture cyanobacteria water circulation treatment equipment which comprises a supporting base, the top of the supporting base is fixedly connected with a purification box, one side of the purification box communicates with a water inlet of an electromagnetic valve, and a water outlet of the electromagnetic valve communicates with a water drainage pipe. Two sides of the purification box are communicated with dosing nozzles, the bottom of the purification box penetrates through and is rotatably connected with a stirring aeration device, one side of the purification box is fixedly connected with an induced draft fan, an air outlet of the induced draft fan is communicated with a first connecting pipe, and the inner wall of the sandy soil settling box is slidably connected with a filtering device. The device is provided with the filtering device, so that the sandy soil filtering rate and the filtering efficiency can be improved in a back-and-forth oscillating and shaking mode, and the device is provided with the stirring and aerating device, so that the contact area between a medicament and sewage can be increased, and the water purifying efficiency is improved.

The present application relates to the technical field of eutrophication water treatment, and discloses a preparation method and application of active substances of algicidal actinomycetes, which comprises the following steps: (1) inoculating actinomycetes into a shaking flask containing a culture medium, wherein the culture medium comprises carbohydrates, salts, glutamic acid and cyanobacteria, and then placing the shaking flask in a culture box for oscillation culture to obtain a first-stage seed liquid; (2) inoculating the first-stage seed liquid into a first-stage seed tank according to a volume fraction of 10-20% to obtain a second-stage seed liquid; (3) inoculating the second-stage seed liquid into a fermentation tank according to a volume fraction of 10-20% to obtain an actinomycete fermentation liquid; and (4) centrifuging the actinomycete fermentation liquid to obtain a supernatant and mixing the supernatant with a stabilizer. Glutamic acid can promote the actinomycetes to secrete more active substances during the fermentation process, and cyanobacteria can enable the actinomycetes to maintain the ability to remove algae during the fermentation process to secrete corresponding active substances. The stabilizer can inhibit the growth of miscellaneous bacteria to maintain the stability of algae removal.

The invention discloses a bottom mud resuspension dimming method for water source algae control, and relates to the technical field of blue-green algae prevention and control, the method comprises the following steps: establishing an experiment system comprising a plurality of groups of independent water body simulation devices, each group of device being provided with a bivariate combination of bottom mud concentration and resuspension frequency; constructing a parameterized extinction model related to the sediment concentration and the sedimentation time based on the real-time illumination data of the vertical layered optical monitoring network; the turbidity of the water body is monitored in real time, when the turbidity exceeds a critical value, a dynamic compensation coefficient is generated, and a theoretical daily light receiving quantity threshold value is increased according to a direct proportion relation; traversing all double-variable combinations, and screening variable combinations which simultaneously meet the condition that the actual daily light receiving quantity is not greater than the theoretical threshold value subjected to turbidity correction and the product of the total bottom mud adding quantity and the mechanical energy consumption is minimized. According to the scheme, the parameter adaptability under the complex hydrological condition is remarkably improved, the theoretical optimal cost boundary is approached, and the algae control interruption risk is reduced.

The application discloses a preparation method of carbon dot material based on blue bacteria, and the carbon dot material is prepared by taking blue bacteria photosystem pigment as raw material. The semiconductor fluorescent carbon dot prepared by the method has stable properties, has wide spectrum absorption to sunlight, can convert ultraviolet light, visible light and infrared light into red light, and has good photoelectric performance. The application further discloses a preparation method of a blue bacteria-based semiconductor fluorescent carbon dot-blue bacteria hybrid, and application of the carbon dot material based on blue bacteria in crop production.

The invention discloses a method and device for improving acidified soil by using nitrogen-fixing cyanobacteria, the device comprises a culture tank, the outer wall of the culture tank is provided with an extension part, the top of the extension part is fixedly provided with a fixed cover, the inner bottom of the culture tank is fixedly provided with a waterproof motor, and the output end of the waterproof motor is fixedly provided with a stirring rod; a flexible silica gel paddle is arranged on the outer wall of the end part of the stirring rod, an aeration assembly is arranged on the culture tank, and the aeration assembly comprises an annular microbubble aeration pipe fixedly mounted on the inner wall of the culture tank; the device has the beneficial effects that the flexible silica gel paddle is matched with the low-speed waterproof motor and the annular micro-bubble aeration assembly, so that the dual effects of mechanical stirring and air-lift uniform mixing are realized, algae clusters can be thoroughly scattered, the algae liquid is uniform, the damage of high-speed hard stirring to algae cells is completely avoided, the activity of nitrogen-fixing blue-green algae is retained to the maximum extent, and the nitrogen-fixing effect of the nitrogen-fixing blue-green algae is improved. The survival rate of the algae is greatly improved.

The present disclosure is method for treating an asphalt roof using the following steps: (1) clearing debris from the roof; applying fused alpha alumina containing aluminum oxide to cyanobacteria stains; and (3) evenly distribute fused alpha alumina on the cyanobacteria stains such that when it rains, dead cyanobacteria molecules are washed away.

The invention discloses a blue-green algae collecting device based on a magnetic carrier and a collecting method of the blue-green algae collecting device, and belongs to the technical field of blue-green algae treatment. The device comprises a liquid storage unit, a magnetic separation unit and a collection unit, the magnetic separation unit is matched with the liquid storage unit, the magnetic separation unit generates a directional magnetic field and acts on mixed liquid in the liquid storage unit, so that magnetic carriers combined with blue-green algae in the mixed liquid are gathered in the acting direction of magnetic field force, and the blue-green algae-magnetic carrier compound is formed. The magnetic carrier is prepared, and polydopamine (PDA) is rich in active groups, so that the affinity and binding force to cyanobacteria cells are enhanced; meanwhile, a buffer solution is used for accurately regulating and controlling pH, the optimal environment for carrier synthesis and blue-green algae combination is guaranteed, and compared with an unmodified carrier, the blue-green algae capture rate is remarkably increased.

The invention provides an observation marking device and method for water quality prediction of a blue-green algae prevention and control area, and relates to the technical field of water quality detection.The observation marking device comprises a mounting plate, a buoy is fixed to the bottom of the mounting plate, a lifting rope is wound around the circle center of the bottom of the mounting plate, a balancing weight is fixed to the bottom of the lifting rope, and a control box is fixed to the circle center of the top of the mounting plate; three groups of photovoltaic panels are mounted at the top of the control box at equal intervals, an antenna is mounted at the upper end of the control box, a plurality of mixing grooves are symmetrically formed in the center of a circle of the surface of the mounting plate, and rotating rings are rotatably mounted on the inner rings, located in the mixing grooves, of the mounting plate through bearings. Water at different depths underwater is sampled and conveyed to the mixing tank on the mounting plate to be mixed with the coloring agent, the blue-green algae content is detected by using the detector, the multiple mixing tanks and the sampling pipes can be prevented from being repeatedly used to influence the detection result, and meanwhile, the device can be arranged on the water surface to be used for long-time water quality detection.

The invention discloses a method for converting a carbon source into ethylene glycol. The method comprises the following steps: providing a plastid, wherein the plastid comprises gene sequences of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6; implanting the plastid into blue-green bacteria by means of electroporation treatment to obtain modified blue-green bacteria; and providing the carbon source to the modified cyanobacteria, so that the modified cyanobacteria converts the carbon source into ethylene glycol, thereby producing chemicals with high economic value while treating the carbon-containing waste gas.

The invention discloses intelligent blue-green algae outbreak emergency treatment equipment and method, and belongs to the technical field of water treatment equipment.The intelligent blue-green algae outbreak emergency treatment equipment comprises a water pumping mechanism installed on a buoyancy base, and further comprises a conveying and filtering mechanism, a collecting mechanism, a drainage mechanism, a chemical adding mechanism and a transmission mechanism. According to the intelligent blue-green algae outbreak emergency treatment equipment, the single motor drives the transverse rod to rotate, then the spiral blades are synchronously driven to convey blue-green algae, the transmission mechanism drives the extrusion assembly and the chemical adding mechanism to operate, multi-link cooperation of blue-green algae conveying, dehydration and chemical adding can be achieved, a traditional framework of respective driving of multiple motors is replaced, and the practicability is high. And the equipment complexity is reduced, the energy consumption is reduced, the fault rate of a motor is also reduced, and meanwhile, all links are synchronously linked, so that the process blockage caused by lagging of a single link can be avoided, and the overall processing efficiency is improved.

The invention provides an inoculum for fermenting flocculating agent PAC-containing cyanobacteria mud and a domestication method and application thereof, and belongs to the technical field of environmental protection. The domestication method comprises the following steps: fermenting residual activated sludge for the first time to obtain anaerobic digestion sludge; mixing the anaerobic digestion sludge with blue-green algae mud for secondary fermentation to obtain blue-green algae mud anaerobic digestion sludge; and boiling the anaerobic digestion sludge of the blue-green algae mud, mixing with the blue-green algae mud for third fermentation, adding the blue-green algae mud in the fermentation process, and monitoring the yield of VFAs until the yield of the VFAs is stable. The inoculum obtained by the domestication method disclosed by the invention can be used for effectively fermenting the flocculating agent PAC-containing cyanobacteria mud, so that the acid production efficiency is improved, and the purposes of reducing and recycling the cyanobacteria mud are achieved.

The invention discloses a blue-green algae carbon quantum dot modified gas diffusion electrode as well as a preparation method and application thereof, and belongs to the technical field of environmental engineering. The preparation method comprises the following steps: preparing nitrogen auto-doped cyanobacteria carbon quantum dots (BGA-CQDs) as an electrode modification material by taking nitrogen-rich cyanobacteria as a precursor through a hydrothermal method, and further mixing the cyanobacteria carbon quantum dots with carbon black, polytetrafluoroethylene (PTFE) and absolute ethyl alcohol to prepare uniform catalyst slurry; the blue-green algae carbon quantum dot modified gas diffusion electrode (CQDs-GDE) is prepared by coating a pretreated carbon felt base material with the blue-green algae carbon quantum dot modified gas diffusion electrode (CQDs-GDE), and after the blue-green algae carbon quantum dot modified gas diffusion electrode (CQDs-GDE) is applied to an electro-Fenton system, TCPP in a water body can be efficiently degraded.

The utility model relates to an offshore enclosure system which comprises a first enclosure belt and a second enclosure belt which are arranged between the interiors of a concave shoreline, a water body is arranged in the concave shoreline, and blue-green algae flows into the surface of the water body towards the inner side of the concave shoreline along the prevailing wind direction; the first enclosure belt is located on the inner side of the second enclosure belt and comprises a plurality of first enclosures arranged side by side, the lower end of the first enclosure belt sinks to the bottom of a water body, and a first interval is formed between every two adjacent first enclosures; the second enclosure belt comprises a plurality of second enclosures arranged side by side, the lower ends of the second enclosures sink to the bottom of the water body, and a second interval is formed between every two adjacent second enclosures, so that the maintenance and replacement cost of single enclosures is reduced, meanwhile, the first interval and the second interval are arranged in a staggered mode, and the first enclosure belt and the second enclosure belt jointly share the impact force of water body flowing; the possibility that the first enclosure belt and the second enclosure belt are impacted and damaged is reduced while the effect of enriching the blue-green algae is achieved, and the service life of the first enclosure belt and the service life of the second enclosure belt are prolonged.

Probes for the detection of toxinogenic cyanobacteria, and the use of at least one pair of these probes in a method for the detection of the toxinogenic cyanobacteria in a sample likely to contain the toxinogenic cyanobacteria. Also, corresponding kits including at least one pair probes specific to toxinogenic cyanobacteria.

The invention belongs to the field of gene engineering, and relates to a general carrier for blue-green algae genome editing based on an endogenous I-D type CRISPR-Cas system and application of the general carrier. The editing vector contains an artificial blue algae I-D type CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) expression unit (mini-CRISPR). On the basis of the general genome editing carrier, blue-green algae endogenous I-D type Cascade is guided to cut a genome target so as to realize genome editing. Exogenous Cas protein does not need to be expressed in gene editing, so that cytotoxicity is avoided; the method is simple to operate, can realize traceless gene editing, and can be used for various gene editing modes, such as gene knockout, knock-in, point mutation, large fragment knockout, endogenous plasmid elimination and the like.

Protoporphyrinogen IX oxidases derived from various organisms or their variants are provided, along with their uses for conferring and / or enhancing herbicide tolerance in plants and / or algae.

This invention relates to the field of waste sludge treatment technology, specifically to an in-situ resource utilization method for a microbial-algae system and its application. The in-situ resource utilization method for the microbial-algae system includes: placing waste sludge, microalgae, and sodium sulfite in an anaerobic fermentation reactor, then adjusting the pH of the mixture and fermenting under anaerobic conditions; wherein the microalgae include at least one of the phyla of green algae, cyanobacteria, and diatoms; and no anaerobic sludge is added to the anaerobic fermentation reactor. This method can effectively utilize the microbial-algae system to increase the yield of volatile fatty acids during anaerobic fermentation in situ, and can also reduce the yield of waste sludge in situ, thereby lowering the subsequent disposal costs of sludge and microalgae in the secondary sedimentation tank.