42results about "Algae products" patented technology

The invention provides an hpRNA interference vector for laver and application of the hpRNA interference vector, and belongs to the technical field of genetic engineering and algae biology. According to the hpRNA interference vector, a binary vector pBI121 is used as a skeleton vector, a laver endogenous Actin promoter and a multiple cloning site segment are inserted, the multiple cloning sites allow target gene segments to be cloned in a forward and reverse repetition mode, the target gene segments are spaced by introns, and therefore an efficient hpRNA structure is formed after transcription, and the efficient hpRNA interference vector is obtained. The gene can be directly used for silencing important genes related to growth and development, metabolic regulation, stress response and the like in the laver. The invention further provides a construction method of the hpRNA interference vector and application of the hpRNA interference vector in silencing laver genes and provides a method for silencing laver gene expression, the gene silencing effect is remarkable, experimental results can be repeated, and a powerful tool is provided for molecular breeding and genetic improvement of laver.

An integrated process that combines Atmospheric Carbon Dioxide removal and sequestration using algae related technologies with the production and distribution of electrical power through conventional or Zero Emission methods and an underground Electric Pipeline along with the production, transportation and processing of oil, natural gas, renewable methane, renewable diesel, renewable gasoline, sustainable aviation fuel, salt water, fresh water, carbon dioxide and related coproducts and services. The process can eliminate all greenhouse gas emissions, while creating Emissions Free Energy, EFE, coproducts such as fresh water, ammonia fertilizers, plastics, chemicals, renewable energy resources, electricity, electrical energy storage, and an electrical driven transportation system.

A textile substrate means (1) for growing seaweeds, kelps, or tunicates The textile substrate means (1) comprises threads of a biodegradable material, and the biodegradable material is of pulp origin. the textile substrate means (1) comprises a band shaped substrate means (10); the band shaped substrate means (10) comprises a woven substrate means (11) where a warp yarn (20) and a weft yarn (30) comprise the biodegradable material, and the woven substrate means (11) is woven with either three, four, five, six or seven weft yarns (30) per cm. The biodegradable material may be viscose or Lyocell. A method for growing seaweeds, kelps, or tunicates, is described. A method for harvesting the seaweeds, kelps, or tunicates and processing the seaweeds, kelps, or tunicates together with the textile substrate means (1), is described.

Provided for are methods of producing triacylglycerol-accumulated microalgae, methods for making bioplastics from triacylglycerol-accumulated microalgae, methods for making alga-mixed plastics, and products including these bioplastics. Methods of triacylglycerol accumulation using centrifugation are also provided. Products such as plastic beads and other consumer products can be made from the bioplastics described herein.

There are disclosed processes and systems of culturing and mixing algal growth medium in at least one open algal aquaculture pond for a continuous flow system without requiring mechanical mixing devices. The process involves constructing an open algal aquaculture pond possessing a fetch selected as a function of a specified wind speed and a wave mixed layer (WML) defined by a specified depth from the surface of the pond; supplying algal growth medium to the open algal aquaculture pond, the pond having at least partially a manmade configuration; and culturing algae in the algal growth medium in the open algal aquaculture pond; and wherein a ratio of the WML of the algal growth medium to a total depth of the algal growth medium in the pond is greater than about 0.2. Also disclosed are uses of the mixed algal aquaculture medium and systems for culturing algae.

There are disclosed processes and systems of culturing and mixing algal growth medium in at least one open algal aquaculture pond for a continuous flow system without requiring mechanical mixing devices. The process involves constructing an open algal aquaculture pond possessing a fetch selected as a function of a specified wind speed and a wave mixed layer (WML) defined by a specified depth from the surface of the pond; supplying algal growth medium to the open algal aquaculture pond, the pond having at least partially a manmade configuration; and culturing algae in the algal growth medium in the open algal aquaculture pond; and wherein a ratio of the WML of the algal growth medium to a total depth of the algal growth medium in the pond is greater than about 0.2. Also disclosed are uses of the mixed algal aquaculture medium and systems for culturing algae.

The invention discloses a kelp gametophyte gene editing method and application, and belongs to the technical field of gene editing, the kelp gametophyte gene editing method comprises the following steps: preparing a kelp gametophyte receptor; assembling an RNP complex and preparing a microcarrier; introducing the RNP compound into a kelp gametophyte by using a gene gun; and recovering culture and screening. According to the technical scheme, the dependency of a microinjection technology on precise instruments and professional operation skills is eliminated, efficient gene delivery can be achieved through a standardized gene gun operation process, the technical threshold is greatly reduced, and the kelp gametophyte gene editing method has remarkable technical advantages. According to the method, the high-throughput characteristic of a gene gun technology is fully exerted, multiple mutants can be obtained through one-time bombardment, the experiment efficiency is greatly improved, and powerful support is provided for kelp large-scale gene function research and molecular breeding.

The invention discloses a genetically engineered kelp-based oral mucosal immune activation type HPV (human papilloma virus) vaccine as well as a preparation method and application thereof, and belongs to the technical field of manufacturing of genetically engineered vaccines. A kelp female gametophyte is modified by a genetic engineering method, parthenogenesis of the kelp female gametophyte is induced, a kelp plant stably expressing HPV L1 protein is obtained, and the oral mucosal immune activated HPV vaccine is prepared. According to the HPV vaccine provided by the invention, natural polysaccharide of kelp is used as an adjuvant and is absorbed through intestinal mucosa, so that the adverse reaction caused by traditional vaccine injection can be reduced while the mucosa immune reaction can be effectively activated. By utilizing the characteristic of rapid growth of the kelp and the advantage that the prepared vaccine does not need cold chain transportation, low-cost production and large-range coverage of the HPV vaccine can be realized, and global prevention and eradication of cervical cancer are expected to be promoted.

An aquaculture system is disclosed that includes a cultivation substrate configured to promote seaweed anchorage formation and seaweed attachment. The cultivation system can include one or more of a nutrient phase, an adhesive, a bioactive agent, and a liquid-containing phase. The cultivation substrate can be patterned. The cultivation system can specifically retain and maintain viability of certain seaweed species, such as dulse, kelp, and nori.

Methane production in ruminant animals may be reduced by improving rumen fermentation efficiency through dietary supplementation of Asparagopsis taxiformis. The present disclosure relates to a method of producing A. taxiformis and uses thereof. In particular forms, the present disclosure relates to methods of producing a tetrasporophyte and a gametophyte of A. taxiformis through environmental manipulations.

The process for bleaching algal biomass comprises at least the following phases of:—supply of at least one algal biomass to be bleached;—mixing of the algal biomass with an aqueous solution to obtain a suspension;—light exposure of the suspension to achieve bleaching.

The present invention relates to cultivating fungus and to materials and / or methods for producing, growing, and / or cultivating fungus.

The invention discloses application of a kelp endogenous promoter in kelp breeding and cultivation and a method, and relates to the technical field of biology. The invention provides a kelp endogenous promoter. The sequence of the kelp endogenous promoter is selected from one of the following sequences: (1) an amino acid sequence as shown in any one of SEQ ID NO: 1-3; (2) a sequence obtained by substituting, deleting or adding one or more nucleotides to the sequence shown in any one of SEQ ID NO: 1 to SEQ ID NO: 3; and (3) a sequence having 80% homology with the sequence as shown in SEQ ID NO: 1-3. The promoter can drive gene specific expression or main expression in a kelp gametophyte, can realize effective expression of a GUS reporter gene in transgenic kelp, and promotes development of kelp genetic engineering and functional genomics research.

The invention refers to a novel procedure for the obtention of seaweed flour from red algae. The process comprises the steps of (a) selection of a red seaweed of interest; (b) cold wash; (c) treatment in brine; (d) hot wash; (e) cold wash; (f) alcoholic wash; (g) wet grinding; (h) pre-dry; (i) dry; and j) dry grinding and sieving. The seaweed flour obtained from this process is an ingredient to food, especially milk-based aliments such as desserts, yogurts, ice cream or whipping cream, although it also may be useful as texturizing and emulsifier in meat or vegan analogues preparations.

Methane production in ruminants can be reduced by improving rumen fermentation efficiency through dietary supplementation with Asparagopsis taxiformis. This disclosure relates to a method for producing A. taxiformis and its use. In a specific form, this disclosure relates to a method for producing tetrasporophytes and gametophytes of A. taxiformis by environmental manipulation.

A system (100) for growing algae comprises: (a) an algae growth and oxygen generation stage (10) comprising: an algae growth medium (14), said algae growth medium (14) receiving carbon dioxide (44) from a source of carbon dioxide (38); and a flow path (18A, 18B, 86, 91) through which algae growth medium flows from a feed end to a harvesting end; and (b) a control system for controlling operation of the closed system combustion stage and algae growth and oxygen generation stage, wherein the control system controls dosing of a nitrate species into algae growth medium (14) along the flow path (18A, 18B, 86, 91).

The invention relates to a method for producing a carbon-negative biomineralised macroorganism, the method comprising the following steps: providing a biomineralising macroorganism comprising an organic phase and a mineral phase containing calcium carbonate; culturing the biomineralising macroorganism, in an atmosphere comprising carbon dioxide, in a culture solution comprising salt water having a salinity of between 3 and 260 PSU and an alkalinity of between 1 mmol / L and 200 mmol / L, wherein, during the culturing step, the biomineralising macroorganism produces calcium carbonate crystals; adding an alkaline feedstock to the culture solution in order to ensure the growth of the calcium carbonate crystals of the biomineralising macroorganism during its biomineralisation; harvesting at least part of the biomineralised macroorganism when one of its average dimensions is greater than or equal to 1 cm.

The application discloses a transient transformation method of Hydrilla verticillata and application thereof. The transformation method comprises the following steps: after an explant of the Hydrilla verticillata is subjected to disinfection treatment, the explant is inoculated into a sterile bud induction culture medium to culture, and sterile seedlings are obtained; a codon-optimized Cry1Ac gene is cloned into a pCAMBIA3301 plant expression vector to obtain a recombinant expression vector pCAMBIA3301-Cry1Ac; the recombinant expression vector pCAMBIA3301-Cry1Ac is introduced into agrobacterium, and the obtained recombinant agrobacterium is used to infect stem tips of the sterile seedlings of the Hydrilla verticillata, and the Hydrilla verticillata is obtained through co-culture. The obtained transgenic Hydrilla verticillata can significantly reduce the survival rate of the larvae of the Crambus leucogonus, effectively reduces the feeding damage of the larvae to the Hydrilla verticillata, and provides a new technical approach for genetic engineering improvement of the submerged plant and biological control of the aquatic pest.

The invention provides a method for efficient and stable genetic transformation of an algae strain by agrobacterium tumefaciens-mediated porphyra yezoensis, and belongs to the technical field of plant transgenosis. According to the method, a mixed suspension of porphyra yezoensis thallus fragments, monospores and thallus plantlets is used as a transformation receptor, and an efficient and stable genetic transformation technology is established through the steps of infection solution optimization, gradient screening culture and the like. The technical problems that existing porphyra yezoensis genetic transformation is low in efficiency, poor in repeatability, difficult in transformant screening and the like are effectively solved, the transformation efficiency can reach 30%-40%, the genetic stability of positive plants is close to 100%, a reliable technical platform is provided for gene function verification, character improvement and molecular breeding of porphyra yezoensis, and important scientific research value and application prospects are achieved.

TITLE: PROCESS FOR FORMING CARBON-NEGATIVE MINERALS USING BIOMINERALIZING MACROORGANISMS The invention relates to a process for producing a carbon-negative biomineralized macroorganism, the process comprising the following steps: supplying a biomineralizing macroorganism comprising an organic phase and a mineral phase containing calcium carbonate; culturing, under an atmosphere comprising carbon dioxide, the biomineralizing macroorganism in a culture solution comprising salt water having a salinity level between 3 and 260 PSU and an alkalinity level between 1 mmol / L and 200 mmol / L; culturing during which the biomineralizing macroorganism produces calcium carbonate crystals; adding an alkaline charge to the culture solution to ensure the growth of the calcium carbonate crystals of the biomineralizing macroorganism during its biomineralization.harvesting at least part of the biomineralized macroorganism when one of its average dimensions is greater than or equal to 1 cm. Figure from the abstract: [Fig.1],

The application provides a method for efficiently and stably transforming algae strains of Porphyra yezoensis by Agrobacterium mediation, and belongs to the technical field of plant transgenesis. The application uses a mixed suspension of leaf thallus fragments, single spores and leaf thallus seedlings of Porphyra yezoensis as a transformation receptor, and establishes an efficient and stable genetic transformation technology through steps such as optimization of an infection solution and gradient screening culture. The application effectively solves the technical problems of low genetic transformation efficiency, poor repeatability and difficulty in screening of transformants of the existing Porphyra yezoensis, and the transformation efficiency can reach 30% to 40%, and the genetic stability of positive plants is close to 100%, thereby providing a reliable technical platform for gene function verification, trait improvement and molecular breeding of Porphyra yezoensis, and having important scientific research value and application prospect.

The invention belongs to the technical field of breeding, and particularly provides a porphyra haitanensis-based mutation breeding method, which comprises the following steps: 1) carrying out ultraviolet radiation on a porphyra haitanensis filament; the invention discloses a method for breeding thalli through mutagenesis, which comprises the following steps of: 1) preparing protonema, 2) treating and irradiating the protonema with a mutagenic agent which comprises nitrosoguanidine and a slow-release solution which comprises bleomycin and pingyangmycin, and 3) screening the protonema subjected to mutagenesis treatment to obtain a mutant strain, and continuously culturing to obtain the thalli. The breeding method has the advantages of high efficiency, high mutation rate and good specificity.

To provide a seaweed-based composition having improved functionality, i.e. having excellent rheological properties.SOLUTION: The present invention relates to a seaweed-based composition comprising water, a seaweed powder and an additional component, the additional component being chosen from the group consisting of glucomannans, galactomannans, native starch and combinations thereof. Preferably, the red seaweed is selected from the families of Gigartinaceae, Bangiophyceae, Palmariaceae, Hypneaceae, Cystocloniaceae, Solieriaceae, Phyllophoraceae and Furcellariaceae or combinations thereof.SELECTED DRAWING: Figure 1

To provide a desired microorganism capable of processing waste water containing a high concentration of ammoniacal nitrogen, which has been difficult to treat with conventional microorganisms.SOLUTION: Provided is a microorganism deposited under Accession No. FERM P-22400. The microorganism has the ability to reduce the concentration of ammoniacal nitrogen in waste water.SELECTED DRAWING: Figure 2

A method for producing a cultured algal biomass comprising an antimethanogenic compound, wherein the method comprises the step of culturing the algal biomass: at a light intensity of from 1 µmols m-2.s-1 to 40 µmols m-2.s-1; in a culture media supplemented with bromide; and / or in a culture media supplemented with vanadium.

Provided for are methods of producing triacylglycerol-accumulated microalgae, methods for making bioplastics from triacylglycerol-accumulated microalgae, methods for making alga-mixed plastics, and products including these bioplastics. Methods of triacylglycerol accumulation using centrifugation are also provided. Products such as plastic beads and other consumer products can be made from the bioplastics described herein.

Methane production in ruminant animals may be reduced by improving rumen fermentation efficiency through dietary supplementation of Asparagopsis taxiformis. The present disclosure relates to a method of producing A. taxiformis and uses thereof. In particular forms, the present disclosure relates to methods of producing a tetrasporophyte and a gametophyte of A. taxiformis through environmental manipulations.

A photobioreactor (PBR) cell comprising a riser portion in the form of a tube; a downcomer portion in the form of a tube; a means of joining the riser portion and the downcomer portion to allow liquid flow; and an aeration means.

To provide a wakame thallus and a method for cultivating the same, enabling reduction of a life cycle during wakame cultivation and promoting production of useful wakame strains.SOLUTION: In one aspect, provided are a wakame thallus having a frond length of 8 cm or more and 20 cm or less and having a sporophyll, and a method for cultivating the same.SELECTED DRAWING: Figure 3