24results about "Algae products" patented technology

The invention provides an hpRNA interference vector for laver and application of the hpRNA interference vector, and belongs to the technical field of genetic engineering and algae biology. According to the hpRNA interference vector, a binary vector pBI121 is used as a skeleton vector, a laver endogenous Actin promoter and a multiple cloning site segment are inserted, the multiple cloning sites allow target gene segments to be cloned in a forward and reverse repetition mode, the target gene segments are spaced by introns, and therefore an efficient hpRNA structure is formed after transcription, and the efficient hpRNA interference vector is obtained. The gene can be directly used for silencing important genes related to growth and development, metabolic regulation, stress response and the like in the laver. The invention further provides a construction method of the hpRNA interference vector and application of the hpRNA interference vector in silencing laver genes and provides a method for silencing laver gene expression, the gene silencing effect is remarkable, experimental results can be repeated, and a powerful tool is provided for molecular breeding and genetic improvement of laver.

There are disclosed processes and systems of culturing and mixing algal growth medium in at least one open algal aquaculture pond for a continuous flow system without requiring mechanical mixing devices. The process involves constructing an open algal aquaculture pond possessing a fetch selected as a function of a specified wind speed and a wave mixed layer (WML) defined by a specified depth from the surface of the pond; supplying algal growth medium to the open algal aquaculture pond, the pond having at least partially a manmade configuration; and culturing algae in the algal growth medium in the open algal aquaculture pond; and wherein a ratio of the WML of the algal growth medium to a total depth of the algal growth medium in the pond is greater than about 0.2. Also disclosed are uses of the mixed algal aquaculture medium and systems for culturing algae.

There are disclosed processes and systems of culturing and mixing algal growth medium in at least one open algal aquaculture pond for a continuous flow system without requiring mechanical mixing devices. The process involves constructing an open algal aquaculture pond possessing a fetch selected as a function of a specified wind speed and a wave mixed layer (WML) defined by a specified depth from the surface of the pond; supplying algal growth medium to the open algal aquaculture pond, the pond having at least partially a manmade configuration; and culturing algae in the algal growth medium in the open algal aquaculture pond; and wherein a ratio of the WML of the algal growth medium to a total depth of the algal growth medium in the pond is greater than about 0.2. Also disclosed are uses of the mixed algal aquaculture medium and systems for culturing algae.

The invention discloses a kelp gametophyte gene editing method and application, and belongs to the technical field of gene editing, the kelp gametophyte gene editing method comprises the following steps: preparing a kelp gametophyte receptor; assembling an RNP complex and preparing a microcarrier; introducing the RNP compound into a kelp gametophyte by using a gene gun; and recovering culture and screening. According to the technical scheme, the dependency of a microinjection technology on precise instruments and professional operation skills is eliminated, efficient gene delivery can be achieved through a standardized gene gun operation process, the technical threshold is greatly reduced, and the kelp gametophyte gene editing method has remarkable technical advantages. According to the method, the high-throughput characteristic of a gene gun technology is fully exerted, multiple mutants can be obtained through one-time bombardment, the experiment efficiency is greatly improved, and powerful support is provided for kelp large-scale gene function research and molecular breeding.

Methane production in ruminant animals may be reduced by improving rumen fermentation efficiency through dietary supplementation of Asparagopsis taxiformis. The present disclosure relates to a method of producing A. taxiformis and uses thereof. In particular forms, the present disclosure relates to methods of producing a tetrasporophyte and a gametophyte of A. taxiformis through environmental manipulations.

The invention discloses application of a kelp endogenous promoter in kelp breeding and cultivation and a method, and relates to the technical field of biology. The invention provides a kelp endogenous promoter. The sequence of the kelp endogenous promoter is selected from one of the following sequences: (1) an amino acid sequence as shown in any one of SEQ ID NO: 1-3; (2) a sequence obtained by substituting, deleting or adding one or more nucleotides to the sequence shown in any one of SEQ ID NO: 1 to SEQ ID NO: 3; and (3) a sequence having 80% homology with the sequence as shown in SEQ ID NO: 1-3. The promoter can drive gene specific expression or main expression in a kelp gametophyte, can realize effective expression of a GUS reporter gene in transgenic kelp, and promotes development of kelp genetic engineering and functional genomics research.

Methane production in ruminants can be reduced by improving rumen fermentation efficiency through dietary supplementation with Asparagopsis taxiformis. This disclosure relates to a method for producing A. taxiformis and its use. In a specific form, this disclosure relates to a method for producing tetrasporophytes and gametophytes of A. taxiformis by environmental manipulation.

A system (100) for growing algae comprises: (a) an algae growth and oxygen generation stage (10) comprising: an algae growth medium (14), said algae growth medium (14) receiving carbon dioxide (44) from a source of carbon dioxide (38); and a flow path (18A, 18B, 86, 91) through which algae growth medium flows from a feed end to a harvesting end; and (b) a control system for controlling operation of the closed system combustion stage and algae growth and oxygen generation stage, wherein the control system controls dosing of a nitrate species into algae growth medium (14) along the flow path (18A, 18B, 86, 91).

The invention relates to a method for producing a carbon-negative biomineralised macroorganism, the method comprising the following steps: providing a biomineralising macroorganism comprising an organic phase and a mineral phase containing calcium carbonate; culturing the biomineralising macroorganism, in an atmosphere comprising carbon dioxide, in a culture solution comprising salt water having a salinity of between 3 and 260 PSU and an alkalinity of between 1 mmol / L and 200 mmol / L, wherein, during the culturing step, the biomineralising macroorganism produces calcium carbonate crystals; adding an alkaline feedstock to the culture solution in order to ensure the growth of the calcium carbonate crystals of the biomineralising macroorganism during its biomineralisation; harvesting at least part of the biomineralised macroorganism when one of its average dimensions is greater than or equal to 1 cm.

The application discloses a transient transformation method of Hydrilla verticillata and application thereof. The transformation method comprises the following steps: after an explant of the Hydrilla verticillata is subjected to disinfection treatment, the explant is inoculated into a sterile bud induction culture medium to culture, and sterile seedlings are obtained; a codon-optimized Cry1Ac gene is cloned into a pCAMBIA3301 plant expression vector to obtain a recombinant expression vector pCAMBIA3301-Cry1Ac; the recombinant expression vector pCAMBIA3301-Cry1Ac is introduced into agrobacterium, and the obtained recombinant agrobacterium is used to infect stem tips of the sterile seedlings of the Hydrilla verticillata, and the Hydrilla verticillata is obtained through co-culture. The obtained transgenic Hydrilla verticillata can significantly reduce the survival rate of the larvae of the Crambus leucogonus, effectively reduces the feeding damage of the larvae to the Hydrilla verticillata, and provides a new technical approach for genetic engineering improvement of the submerged plant and biological control of the aquatic pest.

The invention provides a method for efficient and stable genetic transformation of an algae strain by agrobacterium tumefaciens-mediated porphyra yezoensis, and belongs to the technical field of plant transgenosis. According to the method, a mixed suspension of porphyra yezoensis thallus fragments, monospores and thallus plantlets is used as a transformation receptor, and an efficient and stable genetic transformation technology is established through the steps of infection solution optimization, gradient screening culture and the like. The technical problems that existing porphyra yezoensis genetic transformation is low in efficiency, poor in repeatability, difficult in transformant screening and the like are effectively solved, the transformation efficiency can reach 30%-40%, the genetic stability of positive plants is close to 100%, a reliable technical platform is provided for gene function verification, character improvement and molecular breeding of porphyra yezoensis, and important scientific research value and application prospects are achieved.

TITLE: PROCESS FOR FORMING CARBON-NEGATIVE MINERALS USING BIOMINERALIZING MACROORGANISMS The invention relates to a process for producing a carbon-negative biomineralized macroorganism, the process comprising the following steps: supplying a biomineralizing macroorganism comprising an organic phase and a mineral phase containing calcium carbonate; culturing, under an atmosphere comprising carbon dioxide, the biomineralizing macroorganism in a culture solution comprising salt water having a salinity level between 3 and 260 PSU and an alkalinity level between 1 mmol / L and 200 mmol / L; culturing during which the biomineralizing macroorganism produces calcium carbonate crystals; adding an alkaline charge to the culture solution to ensure the growth of the calcium carbonate crystals of the biomineralizing macroorganism during its biomineralization.harvesting at least part of the biomineralized macroorganism when one of its average dimensions is greater than or equal to 1 cm. Figure from the abstract: [Fig.1],

The application provides a method for efficiently and stably transforming algae strains of Porphyra yezoensis by Agrobacterium mediation, and belongs to the technical field of plant transgenesis. The application uses a mixed suspension of leaf thallus fragments, single spores and leaf thallus seedlings of Porphyra yezoensis as a transformation receptor, and establishes an efficient and stable genetic transformation technology through steps such as optimization of an infection solution and gradient screening culture. The application effectively solves the technical problems of low genetic transformation efficiency, poor repeatability and difficulty in screening of transformants of the existing Porphyra yezoensis, and the transformation efficiency can reach 30% to 40%, and the genetic stability of positive plants is close to 100%, thereby providing a reliable technical platform for gene function verification, trait improvement and molecular breeding of Porphyra yezoensis, and having important scientific research value and application prospect.

A method for producing a cultured algal biomass comprising an antimethanogenic compound, wherein the method comprises the step of culturing the algal biomass: at a light intensity of from 1 µmols m-2.s-1 to 40 µmols m-2.s-1; in a culture media supplemented with bromide; and / or in a culture media supplemented with vanadium.

Methane production in ruminant animals may be reduced by improving rumen fermentation efficiency through dietary supplementation of Asparagopsis taxiformis. The present disclosure relates to a method of producing A. taxiformis and uses thereof. In particular forms, the present disclosure relates to methods of producing a tetrasporophyte and a gametophyte of A. taxiformis through environmental manipulations.

A photobioreactor (PBR) cell comprising a riser portion in the form of a tube; a downcomer portion in the form of a tube; a means of joining the riser portion and the downcomer portion to allow liquid flow; and an aeration means.

To provide a wakame thallus and a method for cultivating the same, enabling reduction of a life cycle during wakame cultivation and promoting production of useful wakame strains.SOLUTION: In one aspect, provided are a wakame thallus having a frond length of 8 cm or more and 20 cm or less and having a sporophyll, and a method for cultivating the same.SELECTED DRAWING: Figure 3

A photobioreactor (PBR) cell comprising a riser portion in the form of a tube; a downcomer portion in the form of a tube; a means of joining the riser portion and the downcomer portion to allow liquid flow; and an aeration means.

The present disclosure provides a high-temperature-tolerant strain of Pyropia yezoensis. The novel high-temperature-tolerant strain of Pyropia yezoensis according to the present disclosure has tolerance for high temperatures and exhibits a high growth rate under land-based cultivation conditions using an artificial light source, and is thus highly useful for land-based cultivation and dealing with increases in seawater temperature.

[0003] A photobioreactor (PBR) cell comprising a riser portion in the form of a tube; a downcomer portion in the form of a tube; a means for connecting the riser portion and the downcomer portion to allow liquid flow; and an aeration means. There is a need for new methods and apparatus for growing algae; or at least methods and apparatus to complement previously known methods and apparatus for growing algae. The present invention seeks to provide one or more of improved or alternative methods and apparatus for growing algae, and improved or alternative algae cultures and compositions.

The present disclosure relates to the field of algal cultivation and biofuels. Particularly, the present disclosure relates to a method of enhancing lipid production during algal culturing. Particularly, the present disclosure relates to a method of enhancing neutral lipid and total lipid production by maintaining algae in a thin layer cultivation system and exposing said algae maintained in the thin layer cultivation system to infra-red (IR) radiation. Said method enhances lipid accumulation in algae, thereby increasing the yield of neutral lipids and total lipids. The method is simple, cost-effective in producing high quantities of algal-derived biofuels, requires shorter time duration for lipid induction and results in no or minimal reduction of biomass.

This application belongs to the field of breeding technology and specifically provides a method for mutagenesis breeding of Porphyra haitanensis, comprising the following steps: 1) irradiating the coniferous bodies of Porphyra haitanensis with ultraviolet light; 2) treating the irradiated coniferous bodies with a mutagen comprising nitrosoguanidine and a slow-release solution comprising bleomycin and bleomycin; and 3) screening the mutagenized coniferous bodies to obtain mutants, which are then cultured to obtain thallus. This breeding method has the advantages of high efficiency, high mutation rate, and good specificity.