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103 results about "Pyruvate decarboxylase" patented technology
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Pyruvate decarboxylase is a homotetrameric enzyme (EC 4.1.1.1) that catalyses the decarboxylation of pyruvic acid to acetaldehyde and carbon dioxide in the cytoplasm of prokaryotes, and in the cytoplasm and mitochondria of eukaryotes. It is also called 2-oxo-acid carboxylase, alpha-ketoacid carboxylase, and pyruvic decarboxylase. In anaerobic conditions, this enzyme is part of the fermentation process that occurs in yeast, especially of the genus Saccharomyces, to produce ethanol by fermentation. It is also present in some species of fish (including goldfish and carp) where it permits the fish to perform ethanol fermentation (along with lactic acid fermentation) when oxygen is scarce. Pyruvate decarboxylase starts this process by converting pyruvate into acetaldehyde and carbon dioxide. Pyruvate decarboxylase depends on cofactors thiamine pyrophosphate (TPP) and magnesium. This enzyme should not be mistaken for the unrelated enzyme pyruvate dehydrogenase, an oxidoreductase (EC 1.2.4.1), that catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA.
Genetically modified cyanobacteria for the production of ethanol, the constructs and method thereof
The invention provides a genetically modified Cyanobacteria having a construct comprising DNA fragments encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) enzymes obtained from the Zymomonas mobilis plasmid pLOI295. The Cyanobacteria are capable of producing ethanol in recoverable quantities of at least 1.7 mumol ethanol per mg of chlorophyll per hour.
Owner:ENOL ENERGY
Genetically modified cyanobacteria for the production of ethanol, the constructs and method thereof
InactiveUS6306639B1Inhibit transcriptionAlgae productsBacteriaPhylum CyanobacteriaKetoacid decarboxylase
The invention relates to the genetic modification of Cyanobacteria for the production of ethanol, and more particularly, to the genetic modification of Cyanobacteria by incorporating the genetic information encoding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh).
Owner:ENOL ENERGY
Production of D-lactic acid with yeast
InactiveUS20070031950A1Increase productivityHigh yieldFungiOxidoreductasesBiotechnologyLactate dehydrogenase
A yeast strain, wherein the yeast strain is transformed with at least one copy of a gene coding for <SMALLCAPS>D< / SMALLCAPS>-lactate dehydrogenase functionally linked to a promoter sequence allowing the expression of the gene in the yeast strain and the yeast strain has undergone disruption of one or more pyruvate decarboxylase genes or pyruvate dehydrogenase genes. Also, a method of producing <SMALLCAPS>D< / SMALLCAPS>-lactic acid including culturing such a yeast strain in a medium and recovering <SMALLCAPS>D< / SMALLCAPS>-lactic acid.
Owner:TATE & LYLE INGREDIENTS AMERICAS INC
Yeast organism producing isobutanol at a high yield
The present invention provides recombinant mircoorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise a modification resulting in the reduction of pyruvate decarboxylase and / or glycerol-3-phosphate dehydrogenase activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
Owner:GEVO INC
Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol
Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
Owner:TRUSTEES OF DARTMOUTH COLLEGE THE
Genetically modified Cyanobacteria for the production of ethanol, the constructs and method thereof
Owner:ENOL ENERGY
Acetyl-coa producing enzymes in yeast
InactiveUS20100248233A1Increase productionFungiMicrobiological testing/measurementHeterologousAcetyl Coenzyme A Synthetase
The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.
Owner:DSM IP ASSETS BV
Selection of ADH In Genetically Modified Cyanobacteria For The Production Of Ethanol
InactiveUS20100003739A1Improve abilitiesIncrease enzyme activityBacteriaBiofuelsPhylum CyanobacteriaCyanobacteria
The present invention discloses genetically-modified cyanobacteria with ethanol-production capabilities enhanced over the currently-reported art, and methods of making such cyanobacteria. The invention provides a genetically modified photoautotrophic, ethanol producing host cell comprising an overexpressed pyruvate decarboxylase enzyme converting pyruvate to acetaldehyde and an overexpressed Zn2+ dependent alcohol dehydrogenase enzyme converting acetaldehyde to ethanol.
Owner:ALGENOL BIOTECH
Ethanol production in recombinant hosts
InactiveUS6849434B2Reduce the amount requiredIncreased ethanol productionBioreactor/fermenter combinationsBiological substance pretreatmentsHeterologousBacteroides
The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable from Zymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.
Owner:UNIV OF FLORIDA RES FOUNDATION INC
Malic Acid Production in Recombinant Yeast
We disclose a recombinant yeast, wherein the yeast is pyruvate decarboxylase enzyme (PDC) activity negative (PDC-negative) and is functionally transformed with a coding region encoding a pyruvate carboxylase enzyme (PYC) wherein the PYC is active in the cytosol, a coding region encoding a malate dehydrogenase enzyme (MDH) wherein the MDH is active in the cytosol and is not inactivated in the presence of glucose, and a coding region encoding a malic acid transporter protein (MAE). We also disclose a method of producing malic acid by culturing such a yeast in a medium comprising a carbon source and a carbon dioxide source and isolating malic acid from the medium.
Owner:TATE & LYLE INGREDIENTS AMERICAS INC
Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor
InactiveUS7326551B2Improve efficiencyImprove filtering effectFungiBacteriaBacteroidesPyruvate carboxylase
The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.
Owner:UNIV OF FLORIDA RES FOUNDATION INC
A genetically engineered bacterium producing 2-phenylethanol and an applying method thereof
ActiveCN106566794AImprove cycle efficiencyDisinhibitionBacteriaMicroorganism based processesPhenethyl alcoholPhenylpyruvate decarboxylase
A genetically engineered bacterium producing 2-phenylethanol and an applying method thereof are disclosed. The recombinant bacterium is constructed by coexpressing aromatic amino acid aminotransferase and exogenous phenylpyruvate decarboxylase (Aro10) (or indole-3-pyruvic acid decarboxylase), phenylethanol dehydrogenase or phenylacetaldehyde reductase in escherichia coli to obtain the recombinant bacterium producing the 2-phenylethanol. Glutamate dehydrogenase is coexpressed on this basis to achieve self-balancing cofactor circulation, thus increasing the yield of the 2-phenylethanol. The conversion ratio of the 2-phenylethanol prepared through the recombinant bacterium is high. The bacterium and the method have important application value in production of the 2-phenylethanol in the future.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Transformant and process for production thereof, and process for production of lactic acid
The present invention relates to a transformant, containing a lactate dehydrogenase gene which is introduced into Schizosaccharomyces pombe as a host, in which a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated.
Owner:ASAHI GLASS CO LTD
Serum creatine kinase detection reagent
ActiveCN104374905AHigh activityEfficient removalMicrobiological testing/measurementBiological material analysisAcyl CoA dehydrogenaseSODIUM DODECYL BENZENE SULFONATE
The invention discloses a creatine jubase detection reagent. The reagent disclosed by the invention consists of a reagent R1 and a reagent R2 according to a volume ratio being 4 to 1, wherein the reagent R1 consists of an imidazole buffer solution, glucose, nano particles, N-acetylcysteine, ethylenediaminetetraacetic acid disodium salt, adenosine diphosphate, coenzyme I, adenine ribonucleotide, pyruvate decarboxylase, 6-phosphogluconic dehydrogenase, hexokinase and sodium dodecyl benzene sulphonate; and the reagent R2 consists of an imidazole buffer solution, phosphocreatine, a preservative and sodium dodecyl benzene sulphonate. Pyruvate decarboxylase and gamma-Fe2O3 nano particles are added into the creatine jubase detection reagent disclosed by the invention, so that interfering substances in a serum sample can be effectively eliminated, metal ions in the serum sample are chelated by matching sodium dodecyl benzene sulphonate and ethylenediaminetetraacetic acid disodium salt, the influence on reaction enzymes is alleviated, and the reagent is a stable, accurate and sensitive detection reagent with high interference resistance.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
Recombinant escherichia coli for producing tyrosol and construction method and application thereof
The invention discloses recombinant escherichia coli for producing tyrosol and a construction method and application thereof and belongs to the technical field of bioengineering. The escherichia coliheterologously expresses a saccharomyces cerevisiae pyruvate decarboxylase gene ARO10* after codon optimization. According to the recombinant escherichia coli, the ARO10* gene is integrated while a lacI locus, a trpE locus, a pabB locus, a pabA locus and a pykF locus of an escherichia coli genome are deleted, and a strain containing multiple copies of the ARO10* gene is obtained. On the basis of the recombinant strain, the ARO10* gene is randomly integrated at the multiple loci, and the ARO10* gene is inserted at a yccX locus so that the strain with high tyrosol yield can be obtained. No inducer nor antibiotic is required for fermentation by using this strain. After 48 hours of fermentation, the yield of tyrosol can reach 28 mM.
Owner:JIANGNAN UNIV
Constitutive expression cassette of Trichoderma reesei, expression vector, and recombinant strain and application thereof
InactiveCN102311951AHigh expressionAvoid it happening againFungiPeptide/protein ingredientsBiotechnologyKetoacid decarboxylase
The invention which relates to the field of genetic engineering provides a constitutive expression cassette of Trichoderma reesei. The expression cassette comprises a foreign target gene, a promoter of a pyruvate decarboxylase gene (Ppdc) of Trichoderma reesei, and a terminator of the pyruvate decarboxylase gene (Tpdc) of Trichoderma reesei, wherein the nucleotide sequence of the Ppdc is represented by SEQ ID NO.1, and the nucleotide sequence of the Tpdc is represented by SEQ ID NO.2. The expression cassette which comprises the sequence of the Ppdc of Trichoderma reesei and the sequence of the Tpdc of Trichoderma reesei can express genes from animals, plant or fungi without induction, and can properly modify expression products, protein expression can be carried out with the highly efficient synthesis and secretion capability of Trichoderma reesei, the protein expression level is high, and excessive foreign proteins in the expression products can be avoided. A recombinant strain of the present invention can highly express xylanase without cellulose, and the xylanase has an important application in the papermaking industry.
Owner:SHENZHEN UNIV
Ethanol production in gram-positive microbes
InactiveUS20050158836A1Increased ethanol productionIncrease ratingsBioreactor/fermenter combinationsBiological substance pretreatmentsHeterologousMicroorganism
The subject invention concerns the transformation of Gram-positive bacteria with heterologous genes which confer upon these microbes the ability to produce ethanol as a fermentation product. Specifically exemplified is the transformation of bacteria with genes, obtainable from Zymomonas mobilis, which encode pyruvate decarboxylase and alcohol dehydrogenase.
Owner:UNIV OF FLORIDA RES FOUNDATION INC
Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor
InactiveUS20080009609A1Improve efficiencyImprove filtering effectFungiBacteriaBacteroidesPyruvate carboxylase
The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.
Owner:UNIV OF FLORIDA RES FOUNDATION INC
Yeast organism producing isobutanol at a high yield
Owner:GEVO INC
Thermophillic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol
Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase
Owner:TRUSTEES OF DARTMOUTH COLLEGE THE
Bacillus coli alcoholic fermentation engineering bacterium and uses thereof
InactiveCN101429488AIncrease filtration rateHigh yieldBacteriaBiofuelsEscherichia coliHigh concentration
The invention relates to a colon bacillus alcohol fermentation engineering bacterium resisting alcohol. A colon bacillus mutant strain highly resisting the alcohol is obtained through a plurality of generations of directional screening; and an alcohol dehydrogenase gene II and a pyruvate decarboxylase gene of zymomonas mobilis are transferred into the mutant strain to obtain the novel colon bacillus alcohol fermentation engineering bacterium. When the engineering bacterium uses pentose and hexose as substrates to ferment for producing the alcohol, the engineering bacterium can still maintain higher fermentation rate under high-concentration alcohol, and has higher transformation rate of the alcohol.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Lactic acid-producing engineering bacteria, constructing method thereof and application thereof
InactiveCN101886048ABreak through the bottleneck of productionBreak through the bottleneckFungiMicroorganism based processesMicrobiologyBacterial strain
The invention discloses lactic acid-producing engineering bacteria, a constructing method thereof and application thereof, and belongs to the field of fermentation engineering. In the genetic engineering bacterial strain provided by the invention, gene segments of lactic dehydrogenase (LDH), which are derived from Bovine, are integrated into S.cerevisiae genome by a homologous recombination method, and the pyruvic decarboxylase (PDC1) gene of the bacterial strain is silenced. The engineering bacterial strain obtained by constructing is used for fermenting and producing the lactic acid; after fermentation for 100 hours, the yield of the lactic acid reaches 15g / L, and the yield of ethanol is reduced by 45 percent less than a parent strain, namely 16.2g / L; and the engineering bacteria has a wide application prospect.
Owner:JIANGNAN UNIV
Yeast organism producing isobutanol at a high yield
Owner:GEVO INC
Creatine kinase isoenzyme detection reagent
ActiveCN104374925AHigh activityEfficient removalBiological testingSodium acetateAntiendomysial antibodies
The invention discloses a creatine kinase isoenzyme detection reagent. The reagent consists of a reagent R1 and a reagent R2 according to the volume ratio of 4 to 1, wherein the reagent R1 consists of an imidazole buffer liquid, glucose, nano particles, N-acetylcysteine, sodium ethylene diamine tetracetate, adenosine diphosphate, cozymase I, ribonucleotide, pyruvate decarboxylase, glucose 6-phosphate dehydrogenase, hexokinase, a goat anti-human CK-M polyclonal antibody and trehalose; the reagent R2 consists of an imidazole buffer liquid, phosphocreatine, sodium dodecyl benzene sulfonate and a preservative. The reagent disclosed by the invention is a creatine kinase isoenzyme detection reagent which is stable, accurate, and high in anti-interference, and has very high clinical application value.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD
Acetyl-coa producing enzymes in yeast
ActiveUS20140030730A1Increase productionMicroorganismsMicrobiological testing/measurementAcetyl Coenzyme A SynthetaseFermentation
Owner:DSM IP ASSETS BV
Recombinant hosts and methods for manufacturing polyhydroxyalkanoate
Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (Ppdc). It was found that the 4HB monomer content in P(3HB-co-4HB) is significantly enhanced to be over 20 mol %, in the range of 8.8 to 23 mol %.
Owner:TIANJIN GREENBIO MATERIAL CO LTD
Enzymatic method for preparing L-xylose
The invention discloses an enzymatic method for preparing L-xylose and belongs to the field of bioengineering. In the method, 2-keto-L-gulonic acid is dissolved in a buffer containing magnesium ions and thiamine pyrophosphate, and pyruvate decarboxylase is added to the buffer for an enzyme-catalyzed reaction at a suitable temperature. After the end of the reaction, the production of the L-xylose is proved by a high performance liquid chromatograph. A new method for producing the L-xylose is found. The method is low in raw material cost, has no pollution to the environment, and has important economic and social benefits.
Owner:SHANGHAI LINKCHEM TECH CO LTD
Method for synthesizing acetoin and derivative thereof through biological method
ActiveCN103725718AAddresses depletion affecting acetoinSolve productivityFermentationVector-based foreign material introductionEmulsion2,3-Butanediol
The invention discloses a method for synthesizing acetoin and a derivative thereof through a biological method, which belongs to the technical field of the molecular biology. The method comprises the steps of introducing pyruvate decarboxylase genes and genes with acetoin enzyme activity into host bacteria to obtain recombination bacteria, producing the acetoin by fermenting the recombination bacteria, and further producing 2,3-butanediol on the basis of the acetoin. By adopting the method, the acetoin and the 2,3-butanediol are successfully generated by fermenting the recombined bacteria strain, and the problem that the yield of the acetoin and the 2,3-butanediol is influenced by the consumption of an intermediate product acetyl emulsion in the natural production process can be solved.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
Construction of a lactobacillus casei ethanologen
An engineered bacterium for producing ethanol from one or more carbohydrates is disclosed. The bacterium can be made by (a) inactivating within a Lactobacillus casei bacterium one or more endogenous genes encoding a lactate dehydrogenase; or (b) introducing into a Lactobacillus casei bacterium one or more exogenous genes encoding a pyruvate decarboxylase and one or more exogenous genes encoding an alcohol dehydrogenase II; or (c) performing both steps (a) and (b). The resulting engineered bacterium produces significantly more ethanol than the wild-type Lactobacillus casei bacterium, and can be used in producing ethanol from a substrate such as biomass that includes carbohydrates.
Owner:UTAH STATE UNIVERSITY +1
Metabolically enhanced cyanobacterial cell for the production of ethanol
A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.
Owner:ALGENOL BIOTECH
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