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98results about How to "Disinhibition" patented technology

Method for producing fatty acid by hydrolyzing lipid through three liquid-phase lipase catalytic systems

The invention discloses a method for producing fatty acid by hydrolyzing lipid through three liquid-phase lipase catalytic systems. The method comprises the following steps: firstly, preparing enzyme solution with the lipase concentration of 5-20000 U / mL, and adding soluble inorganic salts, alcohol / ketonic hydrophilic fraction and lipid to the enzyme solution so as to form three liquid-phase systems; secondly, laying the prepared three liquid-phase systems into an agitated vessel for reaction, standing or centrifuging the systems after the reaction so as to divide the systems into an upper layer, a middle layer and a lower layer, and collecting products in the upper layer so as to obtain fatty acid phase. The method adopts the three liquid-phase systems to extract fatty acid in crude enzyme solution, performs direct hydrolyzing to produce fatty acid, can not only greatly reduce the production cost of enzyme, but also solve the problems of high energy consumption for glycerol and enzyme separation, high cost, high pollution and the like.
Owner:SOUTH CHINA UNIV OF TECH

Myeloma BCMA antigen-targeted transgenic T cell, and preparation method and application thereof

The invention discloses a gene for encoding anti-BCMA chimeric antigen receptor. The nucleotide sequence of the gene is represented by SEQ ID NO:2. The invention also discloses a recombinant expression vector containing the gene, and a myeloma BCMA antigen-targeted transgenic T cell. The transgenic T-cell is a primitive cell containing the recombinant expression vector and knocked out of a PD1 gene or / and a CTLA4 gene, or is a primitive cell with the chromosome being integrated with the gene for encoding anti-BCMA chimeric antigen receptor and knocked out of tbe PD1 gene or / and the CTLA4 gene.A preparation method of the transgenic T-cell comprises the following steps: mixing of gRNA, CRISPR-cas9 mRNA and HDR mix, and electrotransformation recombination of the T cell. The invention furtherdiscloses an application of the myeloma BCMA antigen-targeted transgenic T cell in the preparation of drugs for treating multiple myeloma. In the construction process of carT of, a recognition sequence of EGFR is introduced, EGFR monoclonal antibody Cetuximab is used to eliminate a carT cell if necessary, and PD1 and CTLA4 genes are knocked out to relieve the inhibition effect of the PD1 and CTLA4 genes on the carT cell and enhance the overcoming effect of the carT cell on the inhibition of the tumor microenvironment on immune cell functions.
Owner:YINFENG BIOLOGICAL GRP +1

Efficient integration feed mycotoxins adsorbent and use method thereof

The invention discloses an efficient integration feed mycotoxins adsorbent and a use method thereof. The efficient integration feed mycotoxins adsorbent is characterized by comprising the following components in percentage by weight: 6-80% of composite nanometer modified ultrapure montmorillonite, 10-20% of yeast cell wall extract, 5-15% of crosslinked PVPP (Polyvinylpyrrolidone) and 2.5-5% of compound immune polysaccharides, wherein the yeast cell wall extract comprises mannan oligosaccharide and beta-glucan, and the compound immune polysaccharides comprise epimedium polysaccharides and astragalus polysaccharides. According to the use method of the efficient integration feed mycotoxins adsorbent, the addition in a feed raw material or a compound feed is 0.1-0.5% of the feed weight. The efficient integration feed mycotoxins adsorbent disclosed by the invention blocks reduction of animal production performance, reduction of immunity, increase of sensitivity for various diseases or epidemic diseases and reduction of feed conversion which are caused by mycotoxin contamination of the feed and avoids potential threats to human health due to mycotoxin through a food chain.
Owner:SHANGHAI SANWEI TONGLI BIO TECH

Transgenic T cell of targeted CD30 antigen as well as preparation method and application of transgenic T cell

The invention discloses a transgenic T cell of a targeted CD30 antigen. The transgenic T cell is a primary cell which is integrated with a gene shown as SEQ ID NO:2 and encoding the targeted CD30 antigen, and knocks out a PD1 gene and / or CTLA4 gene, or is a primary cell containing a recombinant lentivirus expression vector (including a gene which is shown as SEQ ID NO:2 and encodes the targeted CD30 antigen and shRNA of a targeted PD1 gene or / and shRNA of a targeted CTLA4 gene); the primary cell is CD4+T cell or CD8+T cell. A preparation method comprises the following steps: firstly, carryingout lentivirus infection on the CD4+T cell or the CD8+T cell; secondly, mixing gRNA, CRISPR-cas9mRNA and HDR, and carrying out electroporation recombination on the T cell to obtain a finished product.According to the transgenic T cell disclosed by the invention, a recognition sequence of an EGFR (Epidermal Growth Factor Receptor) is introduced in carT construction; if necessary, a carT cell can be eliminated by using EGFR monoclonal antibody Cetuximab, the PD1 gene and the CTLA4 gene are knocked out or silenced, inhibition of the gene to the carT cell is eliminated, and the function of overcoming a tumor microenvironment and inhibiting immune cells by the carT cell are enhanced.
Owner:YINFENG BIOLOGICAL GRP

Large-scale preparation method of mesenchymal stem cell factors

The invention relates to a large-scale preparation method of mesenchymal stem cell factors. The large-scale preparation method comprises three steps of invitro culture of mesenchymal stem cells, induction and secretion of the mesenchymal stem cell factors and separation and purification of the mesenchymal stem cell factors, wherein an induction medium required by the step of the induction and secretion of the mesenchymal stem cell factors is prepared from a mixed medium of a DMEM (Dulbecco's Modified Eagle Medium), a PBS buffer solution and an L-alanyl-L-glutamine aqueous solution of which the volume ratio is (30 to 80):(20 to 60):(0.2 to 1.5) and an inducer. According to the large-scale preparation method of the mesenchymal stem cell factors, a single sample source is adopted to culture on a large scale, so that the uniformity of batch products is guaranteed, and the induction medium is adopted in the step of the induction and secretion of the mesenchymal stem cell factors, so that a large quantity of cell factors are stimulated to secrete, a formula is reasonable and effective, the conditions of the whole preparation process are stable and reasonable, and the large-scale preparation method is suitable for large-scale production.
Owner:北京银丰鼎诚生物工程技术有限公司

Efficient scale inhibitor of tail-gas compressor of styrene device and application method thereof

The invention relates to an efficient scale inhibitor of a tail-gas compressor of a styrene device and an application method thereof, comprising hydroxylamine compounds, alcohol amine compounds and phenol compounds. The invention overcomes the disadvantages that the generation of polymer dirt in the tail-gas compressor can not be effectively inhibited by using an ethylbenzene physical spraying method, can thoroughly inhibit and suppress the radical polymerization of the styrene, alpha-methyl styrene, phenyl ethylene and the other activated alkenes in the styrene tail-gas compressor, and has the functions of metallic catalytic coke deposition suppression and detergency and dispersancy. The invention has the advantages of safety and environment protection, energy conservation and low energy consumption and simple use, has no influence on downstream processes, can effectively promote the operational cycle and working load of the styrene tail-gas compressor, reduce energy and material consumption of the styrene device, and can improve the economical and social effects of the whole styrene device.
Owner:北京斯伯乐科技发展有限公司

Method for preparing gardenia blue by utilizing phase-transfer catalysis

The invention discloses a method for preparing gardenia blue by utilizing phase-transfer catalysis. The method comprises the following steps: peeling off gardenia jasminoides fruits, crushing into fruit powder, adding ethanol water for supersonic extraction, filtering, performing vacuum concentration on the filtrate, recycling ethanol, drying the solid obtained through concentration, and dissolving the solid into water so as to obtain a crude geniposide product solution; mixing the crude geniposide product solution with beta-glucosidase to be used as a water phase, performing catalytic hydrolysis reaction under the condition of oscillation or stirring so as to obtain an intermediate product genipin by using an organic solvent as an organic phase; adding a solution of hydrophilic amino acid into the organic phase, subsequently reacting under the condition of oscillation or stirring so as to prepare the gardenia blue; concentrating and drying the water phase in vacuum so as to obtain gardenia blue powder. According to the method, a reaction-extraction technique is utilized, product inhibition and side product degradation reaction when the genipin is prepared by using an ordinary method are effectively prevented, and the conversion rate of geniposide is high, and meanwhile high-purity gardenia blue can be obtained through reverse extraction by using an amino acid solution, and an organic solvent can be recycled.
Owner:NANJING UNIV OF TECH

Method for perstraction of fermented microbial intracellular product with non-ionic surfactant

The invention discloses a method for perstraction of a fermented microbial intracellular product with a non-ionic surfactant. Specifically, during fermentation of a microbial intracellular product, a non-ionic surfactant micellar solution formed by addition of the non-ionic surfactant concentration or a cloud point system formed thereby is adopted as a microbial fermentation medium, by which the intracellular product can be penetrated to an extracellular environment so as to improve the product level of microbial fermentation. Simultaneously, perstraction and fermentation of the microbial intracellular product can be realized. The method of the invention is especially suitable for the fermentation process of an intracellular product, such as production of intracellular microbial enzymes, production of intracellular organic small molecule substances as well as production of intracellular oil compounds, etc. The method provided in the invention effectively eliminates intracellular product inhibition, enhances the concentration of a microbial fermentation product, and adjusts the composition of a microbial fermentation secondary metabolite, thus improving the volume yield of microbial fermentation as well as the efficiency of intracellular product fermentation, product release and other processes.
Owner:JECHO BIOPHARM CO LTD

Production process for producing gamma-cyclodextrin by biological method

The invention relates to a production process for producing gamma-cyclodextrin by a biological method and belongs to the technical field of cyclodextrin production. The production process solves the technical problems to provide the process for producing gamma-cyclodextrin by the biological method, and the production cost of the gamma-cyclodextrin is reduced. The production process has the technical scheme that the gamma-cyclodextrin glucosyltransferase from alkalophilic bacillus is adopted for producing the gamma-cyclodextrin, the starch pulp regulation is carried out according to the concentration being 10 percent, the materials are stirred for 5 to 15 minutes under the condition being 60 to 90 DEG C, the temperature is set to be 40 to 60 DEG C, the pH is regulated to be about 10.0, the gamma-cyclodextrin glucosyltransferase is added according to a quantity of 1 to 10 units for one gram of starch, after 2 to 5 percent (w / v) organic solvents are added, the sufficient reaction is carried out for 8 to 10 hours, the organic solvents are recovered, and the gamma-cyclodextrin is obtained by adopting a crystallization method.
Owner:JIANGNAN UNIV

Regeneration method for urban sewage by continuous flow

ActiveCN103112999ASolve denitrificationSolve the contradiction between releasing phosphorus and competing for organic carbon sourceMultistage water/sewage treatmentContinuous flowOxygen
The invention belongs to the technical field of water environment restoration and particularly relates to a regeneration method for urban sewage by continuous flow. In view of the problems existing in a conventional simultaneous nitrogen and phosphorus removal process of serious shortage of carbon sources due to competition for carbon sources in nitrogen and phosphorus removal, big differences in sludge ages of a denitrifier and phosphorus removal bacteria and demand for dissolved oxygen, insufficient alkalinity of sewage, difficulty in giving consideration to nitrogen and phosphorus removal effects concurrently and the like, through overall planning from the process flow and optimization of tasks of all processing units, the regeneration method can be used for removing phosphorus and organic matters by an anaerobic-aerobic process, partially oxidizing ammonia and nitrogen into nitrite nitrogen by a multistage completely hybrid nitrosation process, and realizing autotrophic denitrification by using an upward-flow anaerobic ammonia oxidation biofilter so as to achieve high-efficient low-carbon treatment and regeneration of the sewage, with effluent COD less than 45 mg / L, BOD less than 10 mg / L, TP less than 0.4 mg / L, and TN less than 10 mg / L, so that the treated sewage can reach the national primary standard A.
Owner:BEIJING UNIV OF TECH

Preparation method of CAR (Cheimeric Antigen Receptors)-T cells for co-expressing IL18 (Interleukin 18) and CCL19 (Chemokine C-C motif ligand 19) protein and targeting MUC1 gene

The invention relates to a preparation method of CAR (Cheimeric Antigen Receptors)-T cells for co-expressing IL18 (Interleukin 18) and CCL19 (Chemokine C-C motif ligand 19) protein and a targeting MUC1 gene. The preparation method comprises the following steps: transfecting DC<4+> / CD<+8> positive cells by lentivirus containing IL18 and CCL19 protein coding sequences and targeting MUC1 CAR; screening and culturing amplified and transfected T cells to obtain the CAR-T cells for co-expressing IL18 and CCL19 protein and the targeting MUC1 gene. According to the preparation method provided by the invention, the proliferation capability and the life cycle of the CAR-T cells are enhanced through expressing third cell signals IL18 and CCL19, improving the osmosis effect of the CAR-T cells in solidtumors and maintaining the killing effect of the CAR-T cells on the solid tumors. Furthermore, the co-expressed IL18 and CCL19 are connected through a 2A short peptide and can be expressed respectively.
Owner:杭州荣泽生物科技集团有限公司

Transgenic T cell of targeted CD19 antigen as well as preparation method and application of transgenic T cell

The invention discloses a transgenic T cell of a targeted CD19 antigen. The transgenic T cell is a primary cell which is integrated with a gene shown as SEQ ID NO:8 and encoding the targeted CD19 antigen in chromosome, and knocks out a PD1 gene and / or CTLA4 gene, or is a primary cell which is integrated with a gene shown as SEQ ID NO:2 and encoding a targeted PSA-NCAM receptor ,and a gene shown asSEQ ID NO:8 and encoding the targeted CD19 antigen in the chromosome; the primary cell is CD4+T cell or CD8+T cell. The invention also discloses application of the transgenic T cell of the targeted CD19 antigen in preparation of a medicine for treating malignant B cell lymphoma. According to the transgenic T cell disclosed by the invention, a recognition sequence of an EGFR (Epidermal Growth Factor Receptor) is introduced in carT construction; if necessary, a carT cell can be eliminated by using EGFR monoclonal antibody Cetuximab, the PD1 gene and the CTLA4 gene are silenced or knocked out, inhibition of the gene to the carT cell is eliminated, and the function of overcoming a tumor microenvironment and inhibiting immune cells by the carT cell are enhanced.
Owner:YINFENG BIOLOGICAL GRP

Medicine for improving production performance of sow and preparation method and application thereof

The invention discloses a medicine for improving the production performance of a sow and a preparation method and application thereof, and belongs to the technical field of veterinary medicines. The medicine for improving the sow production performance is prepared from 2 to 10 parts by weight of vitamins, 4 to 15 parts by weight of plant extract, 2 to 6 parts by weight of an enzyme preparation, 2to 6 parts by weight of amino acids, 1 to 5 parts by weight of a microbial ecological agent and 1 to 6 parts by weight of mannooligosaccharide. The medicine is easy to use, is effective and safe, hasno residue, effectively reduces the sow production process, promotes the postpartum recovery of the sow, improves ovulation, prolongs the sow production life, promotes the sow mammogenesis and milk secretion, improves milk quality, improves the sow production quality, improves the survival rate of the piglet through use in a lactation period and guarantees the healthy and fast growth of piglets.
Owner:山东迅达康生物科技有限公司 +1

Method for inducing blood lymphocyte of litopenaeus vannamei to generate UPR (unfolded protein response) by means of RNA interference technology

The invention provides a method for inducing blood lymphocyte of litopenaeus vannamei to generate UPR (unfolded protein response) by means of an RNA interference technology. The method comprises the following steps: (1) synthesizing dsLvBip with sequence specificity according to a cDNA sequence of a Bip gene of litopenaeus vannamei; and (2) diluting dsLvBip, controlling the injection volume at 50 mu L and injecting dsLvBip into the litopenaeus vannamei according a dosage of 1 mu g / g. The method provided by the invention can be used for simply, conveniently, rapidly, efficiently and specifically inducing blood lymphocyte of litopenaeus vannamei to generate UPR.
Owner:SUN YAT SEN UNIV

Device and method for biologically manufacturing succinic acid by continuous crystallization

The invention relates to a device and a method for biologically manufacturing succinic acid by continuous crystallization, and the device comprises: a bioreactor (1), a cross-flow membrane module (2), and a crystallization separation tank (3); a front end of the cross-flow membrane module (2) is communicated with the bioreactor (1) through a pipeline; a filtering end of the cross-flow membrane module (2) is communicated with a bottom of the crystallization separation tank (3); a top of the crystallization separation tank (3) is communicated with the bioreactor (1); therefore, a loop is formed in the device, and the continuous fermentation and crystallization of succinic acid is realized; a peristaltic pump (9) is disposed between the bioreactor (1) and the cross-flow membrane module (2); a back end of the cross-flow membrane module (2) is communicated with the bioreactor (1), which realizes the circulation of the flowing fermentation liquid between the two. The device and the method for biologically manufacturing succinic acid by continuous crystallization of the invention establish a coupling system for low-temperature crystallization in-situ extraction of succinic acid and fermentation and separation, and meet requirements for disinfection sterilization and pollution-free operation.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI

Method for expressing and purifying recombinant ethanol oxidase in pichia pastoris

The invention discloses a method for expressing and purifying recombinant ethanol oxidase rAOX. According to the method, an ethanol oxidase-poly-L-histidine expression vector pPIC9K-AOX-HIS is constructed first by a molecular biological technique, then the expression vector pPIC9K-AOX-HIS is linearized and transferred to pichia pastoris GS115, and thus, pichia pastoris engineering bacteria capable of expressing active recombinant ethanol oxidase are obtained, and part of recombinant ethanol oxidase can be secreted and expressed. Through optimization of the expression conditions of recombinant ethanol oxidase, the ethanol oxidase produced by the engineering bacteria may reach 1,862U / L. Meanwhile, the secretion and expression of the ethanol oxidase and the introduction of a poly-L-histidine tag simplify the purification process of the recombinant ethanol oxidase. According to the result of tests, the recombinant ethanol oxidase expressed by the engineering bacteria has much higher thermostability than pichia pastoris wide type ethanol oxidase. The operation in the whole process of the method is simple, so the method is suitable for industrial production and can create certain economic benefit.
Owner:ZHEJIANG DEQING HUINING BIOTECH

Lentinan powder for preventing and/or assisting in treating tumors and extraction process thereof

The invention provides lentinan powder for preventing and / or assisting in treating tumors and an extraction process thereof. The invention provides an extraction method of lentinan. The method includes a step of contacting a solution containing lentinan or crude-extracted lentinan powder with alcohol solutions with different concentrations to precipitate lentinan. The invention further provides lentinan obtained by the method and an application thereof in preparing medicines for preventing and / or treating the tumors. The invention further provides a medicine or a food composition containing the lentinan obtained by the method. The lentinan obtained by the method of the invention has an excellent effect of preventing and treating the tumors.
Owner:陶宁

Composite chemical for degrading pollutant benzoquinone in wastewater and method for degrading pollutant benzoquinone in wastewater

The invention discloses a composite chemical for degrading pollutant benzoquinone in wastewater. The composite chemical consists of a stabilizer A, a microorganism energy supply material B and a flocculating agent C, wherein the mass ratio of mixing the stabilizer A to the microorganism energy supply material B to the flocculating agent C is (20-35) to (465-480) to (300-500). The method for degrading pollutant benzoquinone in wastewater with the composite chemical comprises the following steps: regulating pH value of wastewater; then, adding microorganism energy supply material B and flocculating agent C in the composite chemical to the wastewater, uniformly mixing, and adding stabilizer A in the composite chemical to the wastewater, wherein the adding concentration of the composite chemical is controlled to be not less than 120mg / L; reacting fully after mixing, and carrying out microorganism treatment so as to degrade the pollutant benzoquinone. The composite chemical disclosed by the invention has a good benzoquinone degradation effect and is stable, and is low in treatment cost and easy in implementation.
Owner:CENT SOUTH UNIV

Dry anaerobic fermentation process and device for reducing ammonia nitrogen accumulation

The invention discloses a dry anaerobic fermentation process for reducing ammonia nitrogen accumulation. The dry anaerobic fermentation process is characterized by comprising the following steps of: detecting ammonia nitrogen concentration of fermentation liquor in a dry anaerobic fermentation reaction tank; when the ammonia nitrogen concentration of the fermentation liquor is less than a preset value, directly reflowing into the dry anaerobic fermentation reaction tank; and when the ammonia nitrogen concentration of the fermentation liquor reaches the preset value, removing ammonia nitrogen of the fermentation liquor through zeolite. The invention also discloses a device which is used for implementing the process. According to the dry anaerobic fermentation process and device, the suppression of the high-concentration ammonia nitrogen on the anaerobic fermentation reaction is reduced, the gas producing speed of the system is increased, and the replaced zeolite which adsorbs the high-concentration ammonia nitrogen can also be used as a soil conditioner.
Owner:CHINESE RES ACAD OF ENVIRONMENTAL SCI

Method for producing lactic acid and simultaneously realizing coupling preparation of GABA (gamma-aminobutyric acid)

The invention discloses a method for producing lactic acid and simultaneously realizing coupling preparation of GABA (gamma-aminobutyric acid). The method comprises the steps of inoculating rhizopus oryzae into a fermentation culture medium to perform fermentation, adding glutamic acid decarboxylase, pyridoxal phosphate and L-sodium glutamate into a fermentation solution when the pH value of the fermentation solution is less than 4.8, then continuously adding L-sodium glutamate into the fermentation solution, and generating GABA of glutamic acid decarboxylase by utilizing the vitality of catalyzing glutamic acid to consume H+ in an environment so as to maintain the pH value of the fermentation solution at 4.8-6.0 and achieve the purposes of relieving acid inhibition against fermentation of lactic acid and realizing simultaneous production of lactic acid and GABA. The invention provides a new method for relieving the inhibition of a substrate for fermentation of lactic acid; furthermore, in the fermentation process, the coupling production of a functional compound, namely gamma-aminobutyric acid, is realized, so that the yield of lactic acid is increased, and the economy of the process is further improved.
Owner:SHANDONG YANGCHENG BIOLOGY TECH CO LTD

Traditional Chinese medicine for treating aplastic anemia and its preparation process

The invention discloses a medicinal Chinese medicine for curing regenerative obstructive anaemia and its preparing technique, prepared in proportion of red ginseng tails, Radix astragali, Radix rehmanniae preparata, dodder seed, alter slice, moxibustion tortoise plastron, medlar, Malaytea Scurfpea Fruit, Prepared Common Monkshood Daughter Root, and cinnamon. And the preparing technique: decocting the above ten raw materials twice and combining the two filtrates; then concentrating, alcohol-depositing, cooling to room temperature and making into cream; drying the cream and then making powder spraying and filling into capsules; or adding dextrin or starch to the cream to make into soft material, and finally screening and drying the soft material to make into electuary or tablets. It can not only regulate mediation disorder of human immunity, stimulate the hemopoiesis recovery of human marrow, and have remarkable curative effect on the regenerative obstructive anaemia but also has considerably good curative effect on other refractory anaemias, as well as the symptoms caused by various reasons, such as marrow inhibition, blood cell reduction, etc. And it almost has no toxicity and side effect, safe and reliable, low-cost.
Owner:武汉芄焓药物研发有限公司

Method for preparing gardenia blue by utilizing phase-transfer catalysis

The invention discloses a method for preparing gardenia blue by utilizing phase-transfer catalysis. The method comprises the following steps: peeling off gardenia jasminoides fruits, crushing into fruit powder, adding ethanol water for supersonic extraction, filtering, performing vacuum concentration on the filtrate, recycling ethanol, drying the solid obtained through concentration, and dissolving the solid into water so as to obtain a crude geniposide product solution; mixing the crude geniposide product solution with beta-glucosidase to be used as a water phase, performing catalytic hydrolysis reaction under the condition of oscillation or stirring so as to obtain an intermediate product genipin by using an organic solvent as an organic phase; adding a solution of hydrophilic amino acid into the organic phase, subsequently reacting under the condition of oscillation or stirring so as to prepare the gardenia blue; concentrating and drying the water phase in vacuum so as to obtain gardenia blue powder. According to the method, a reaction-extraction technique is utilized, product inhibition and side product degradation reaction when the genipin is prepared by using an ordinary method are effectively prevented, and the conversion rate of geniposide is high, and meanwhile high-purity gardenia blue can be obtained through reverse extraction by using an amino acid solution, and an organic solvent can be recycled.
Owner:NANJING TECH UNIV

Comprehensive extraction method for fructus gardeniae

ActiveCN106432399AReduce difficultyIncrease conversion rates andNatural dyesSteroidsSolventAlglucerase
The invention discloses a comprehensive extraction method for fructus gardenia. The comprehensive extraction method comprises the following steps: carrying out ultrasonic extraction on the fructus gardenia by taking water as a solvent so as to obtain supernate and solid residues, filtering the supernate by virtue of a micro-filtration membrane to obtain filtrate A containing geniposide and a gardenia yellow pigment, mixing the filtrate A with beta-glucosidase liquid as a water phase, converting geniposide in the filtrate A into genipin by virtue of beta-glucosidase in a two-phase system, and transferring the genipin into the organic phase, namely an organic solvent, so as to react to obtain a gardenia blue pigment; separating and purifying the gardenia yellow pigment in the residual water solution; and extracting the solid residues with ethanol to obtain supernate, and carrying out separation and purification, so as to obtain ursolic acid. The comprehensive extraction method has the beneficial effects that the cost is low, the process is simple and practical, efficient paths for purifying the gardenia yellow pigment and converting the geniposide into the gardenia blue pigment are realized, and the extraction of ursolic acid in the solid residues, the recycling and repeated use of fixed beta-glucosaccharase and the maximum utilization of the fructus gardenia are realized.
Owner:NANJING UNIV OF TECH

Mutant of gamma-glutamine methylamine synthetase, and encoding gene, amino acid sequence and application of mutant

The invention discloses a mutant of gamma-glutamine methylamine synthetase, and an encoding gene, amino acid and application of the mutant to production of theanine. Specifically, the mutant of the gamma-glutamine methylamine synthetase is obtained through a genetic engineering method. The optimum temperature of enzyme is increased by 10 DEG C while the thermal stability of the gamma-glutamine methylamine synthetase is improved, the mutant can play a very good catalytic effect without being in a buffer salt solution, and meanwhile, the theanine is efficiently synthesized by utilizing the mutant through a method of coupling polyphosphate kinase; and according to the method, adenosine triphosphate (ATP) regeneration can be implemented while the theanine is synthesize by catalysis, and an effective way is provided for enzymatic industrial production of the theanine.
Owner:INNOBIO CORP LTD

Preparation method of tumor-enhanced tumor-infiltrating lymphocytes

The invention discloses a preparation method of tumor-enhanced tumor-infiltrating lymphocytes. The preparation method comprises the following steps: (1) extracting tumor-infiltrating lymphocytes TILs from tumor tissues; (2) sorting and enriching PD1 positive T cells in the TILs; (3) integrating a PBR transformed fusion protein and an IL-15 super compound into the PD1 positive T cell at one time; and (4) carrying out multiplication culture to obtain the tumor enhanced TILs. The preparation method has the advantages that (1) by sorting the PD1 positive T cells, the proportion of tumor-specific T cells is increased; (2) the designed PBR fusion protein can relieve the inhibition from a PD1 pathway in a tumor microenvironment and improve the killing ability of the TILs to tumor cells; (3) the IL-15 super compound not only can improve the tumor killing activity of T cells, but also can stimulate other immune cells in the body, such as endogenous NK, T cells and the like, and accelerates the removal of the tumor cells in the body; and (4) the added 'suicide gene' R structure solves the problem of subsequent clinical safety after T cell gene modification.
Owner:THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE

Engineering bacterium for producing guanidinoacetic acid as well as construction method and application thereof

The invention discloses a genetically engineered bacterium for producing guanidinoacetic acid as well as a construction method and an application of the genetically engineered bacterium. The inventionprovides the construction method of guanidinoacetic acid-producing engineering bacteria, which comprises the following steps: introducing guanidinoacetic acid synthesis-related gene L-arginine: glycine amidinyl transferase encoding gene into host bacteria to obtain guanidinoacetic acid-producing engineering bacteria; wherein the host bacteria are escherichia coli or mutant escherichia coli. According to the invention, an AGAT catalytic reaction which does not exist in the background is constructed in escherichia coli by a method of expressing foreign protein. Meanwhile, by means of plasmid overexpression of protein, replacement of promoters on chromosomes, gene knockout and the like, the flow of an ornithine circulating metabolic pathway of escherichia coli is increased, inhibition of ornithine on AGAT catalytic reaction is relieved, arginine is recycled through a whole-cell catalysis method to produce guanidinoacetic acid, and good environmental and economic prospects are achieved.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI

Chimeric antigen receptor, immune cell modified by chimeric antigen receptor and application of immune cell to treatment of advanced pancreatic cancer

The invention provides a chimeric antigen receptor, an immune cell modified by the chimeric antigen receptor and application of the immune cell to treatment of advanced pancreatic cancer. The chimericantigen receptor is formed by sequentially connecting a hinge region of CD8leader, scFv(TGF-beta), T2A, CD8leader, scFv(GPC-1) and CD8, a CD28 transmembrane-stimulus structural domain and a CD3-zetastimulus signal transduction region in series; a preparation method of the immune cell modified by the chimeric antigen receptor includes immune cell separation, and infection with lentiviral plasmids; Two antibodies are carried in the chimeric antigen receptor; and by applying the immune cell provided by the invention to treatment of the advanced pancreatic cancer, the in-vitro killing efficiencyof positive pancreatic cancer cells reaches 90% or above, and in the aspect of in vivo killing efficiency, it can be seen by nude mouse experiments that the tumor is rapidly shrunk, and the nude mouse tumor completely disappears after 21 days.
Owner:山东仁济生物科技有限公司

Composition for removing tumor immunosuppression and application thereof

The invention discloses a composition for removing tumor immunosuppression and application thereof. The composition provided by the invention comprises following (a) to (e) components or coded nucleicacid thereof: (a) IL-15, a mutant thereof or functional fragments thereof; (b) an IL-15 receptor alpha, a mutant thereof or functional fragments thereof; (c) IL-12, a mutant thereof or functional fragments thereof; (d) TGF-beta regulatory peptide, a mutant thereof or functional fragments thereof; and (e) PD1-CD80 fusion protein, a mutant thereof or functional fragments thereof. According to the composition provided by the invention, the immune response of T lymphocytes can be improved synergistically.
Owner:BEIJING TRICISIONBIO THERAPEUTICS INC
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