Production process for producing gamma-cyclodextrin by biological method

A production process, cyclodextrin technology, applied in the production process field of γ-cyclodextrin by biological method, can solve the problems of lack of γ-cyclodextrin production process, low solubility of β-cyclodextrin, low water solubility, etc. , to achieve the effects of low cost, release of product inhibition, and short reaction cycle

Active Publication Date: 2012-12-19
JIANGNAN UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the three commonly used cyclodextrins, β-cyclodextrin has the smallest water solubility and is easy to obtain by fractional crystallization. Therefore, β-cyclodextrin is widely prepared and widely used in production, but β-cyclodextrin Due to the low solubility of β-cyclodextrin, its application in drug inclusion is limited. At present, scientific and technological workers are working on improving the solubility of β-cyclodextrin.
The main problems of the current biological production of γ-cyclodextrin are: 1. the lack of γ-cyclodextrin glucosyltransferase (γ-CGTase) with excellent properties and high enzymatic activity; 2. the lack of γ-cyclodextrin corresponding Production process, in view of the high solubility of γ-cyclodextrin in water, it is necessary to develop a set of economical and efficient separation and extraction methods

Method used

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  • Production process for producing gamma-cyclodextrin by biological method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Raw material pretreatment:

[0027] According to the concentration of 10%, the potato starch is slurried, and stirred at 65°C for 10 minutes to make the starch granules fully absorb water and swell;

[0028] Enzymatic production process:

[0029] After pretreatment, set the temperature to 50°C, adjust the pH to 10.0, add γ-CGTase at a rate of 6 units per gram of starch, then add 5% (w / v) cyclododecanone, and fully react for 9 hours ;

[0030] The extraction process of γ-cyclodextrin:

[0031] After killing the enzyme in a boiling water bath, filter the reaction liquid, wash the filter cake 2-3 times with distilled water, collect the filter cake, including the precipitate formed by cyclododecanone and γ-cyclodextrin and unreacted starch; Redissolve in water, and then use steam distillation to remove cyclododecanone. After the distillation is over, filter the distillate to remove unreacted starch to obtain an aqueous solution of γ-cyclodextrin; evaporate and concentrat...

Embodiment 2

[0037] Raw material pretreatment:

[0038] Same as Example 1

[0039] Enzymatic production process:

[0040] After pretreatment, set the temperature to 40°C, adjust the pH to 10.0, add γ-CGTase at a rate of 3 units per gram of starch, and then add 4% (w / v) cyclododecanone to the reaction system, fully Reaction for 10 hours;

[0041] The extraction process and detection method of γ-cyclodextrin:

[0042] Same as Example 1

[0043] The results are shown in Table 1. The total conversion rate of starch is as high as 55%, the proportion of γ-cyclodextrin in the product is 50%, and the proportion of β-cyclodextrin is 5%.

Embodiment 3

[0045] Raw material pretreatment:

[0046] Same as Example 1

[0047] Enzymatic production process:

[0048] After pretreatment, set the temperature to 60°C, adjust the pH to 10.0, add γ-CGTase at a rate of 10 units per gram of starch, and then add 2.6% (w / v) cyclododecanone to fully react for 8 Hour;

[0049] The extraction process and detection method of γ-cyclodextrin:

[0050] Same as Example 1

[0051] The results are shown in Table 1. The total conversion rate of starch is as high as 52%, the proportion of γ-cyclodextrin in the product is 48%, and that of β-cyclodextrin is 4%.

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Abstract

The invention relates to a production process for producing gamma-cyclodextrin by a biological method and belongs to the technical field of cyclodextrin production. The production process solves the technical problems to provide the process for producing gamma-cyclodextrin by the biological method, and the production cost of the gamma-cyclodextrin is reduced. The production process has the technical scheme that the gamma-cyclodextrin glucosyltransferase from alkalophilic bacillus is adopted for producing the gamma-cyclodextrin, the starch pulp regulation is carried out according to the concentration being 10 percent, the materials are stirred for 5 to 15 minutes under the condition being 60 to 90 DEG C, the temperature is set to be 40 to 60 DEG C, the pH is regulated to be about 10.0, the gamma-cyclodextrin glucosyltransferase is added according to a quantity of 1 to 10 units for one gram of starch, after 2 to 5 percent (w/v) organic solvents are added, the sufficient reaction is carried out for 8 to 10 hours, the organic solvents are recovered, and the gamma-cyclodextrin is obtained by adopting a crystallization method.

Description

technical field [0001] The invention relates to a production process for producing gamma-cyclodextrin by a biological method, belonging to the technical field of cyclodextrin production. Background technique [0002] Cyclodextrins (Cyclodextrins, usually abbreviated as CD) are a class of D-glucopyranose units connected end-to-end by α-1,4-glycosidic bonds generated by starch or polysaccharides under the action of cyclodextrin glucosyltransferase. The general term for cyclic compounds, common molecules with 6, 7 and 8 glucose units, are called α-, β- and γ-cyclodextrin, respectively. Due to its special structure, cyclodextrin can embed many organic and inorganic compounds, as a quality improver, stabilizer, drug carrier and adsorbent, etc., so it is used in food, medicine, chemistry, analytical chemistry, agricultural environmental protection, Fields such as textile technology and biotechnology have a wide range of applications. Among the three commonly used cyclodextrins, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12R1/01
Inventor 吴敬王磊吴丹陈坚
Owner JIANGNAN UNIV
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