The invention relates to a method using ocean alpha-cyclodextrin glucosyltransferase to convert starch so as to prepare alpha-cyclodextrin. The method includes: performing starch gelatinization, adding the alpha-cyclodextrin glucosyltransferase at 55 DEG C, adding n-decanol to perform reaction, filtering reaction products, repeatedly washing filter cake with distilled water, and colleting the filter cake; adding anhydrous ethanol, performing stirring reaction under room temperature for 5 hours, performing static separatory filtration in a separating funnel, and evaporating lower liquid to obtain the alpha-cyclodextrin. The method has the advantages that the method is simple in flow, short in cycle and high in conversion rate, and the obtained alpha-cyclodextrin is high in purity and widely applicable to fields such as food, medicine, agriculture, spinning, environment protection, cosmetics, biotechnology and analytic chemistry.