Method for purifying fructose oligosaccharide

A technology of fructooligosaccharides and purification methods, which is applied in the direction of oligosaccharides, chemical instruments and methods, sugar derivatives, etc., can solve the problems of low purity of oligosaccharides, and achieve the effects of high purity, improved purity, and high energy saving

Active Publication Date: 2014-08-13
完美(广东)日用品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above two production processes, monosaccharides can be removed through the nanofiltration membrane during the downstream separation process. However, under the premise of ensuring that the fructan with a degree of polymerization of 3-9 does not pass through the nanofiltration membrane, a large amount of disaccharides are also retained, resulting in the final The purity of the product oligosaccharides is low, so choosing a suitable way to reduce the content of disaccharides in the fructo-oligosaccharides without affecting the GFn-type fructo-oligosaccharides with a degree of polymerization greater than 2, the preparation of high-purity fructo-oligosaccharides has become the industry and researchers research hotspots

Method used

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  • Method for purifying fructose oligosaccharide
  • Method for purifying fructose oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Get two different grades of primary fructo-oligosaccharide mixture 100mL (No. 1 and No. 2 purity are 50% and 80% respectively, sucrose content is 0.1mol / L) in enzyme reactor, glucosyltransferase is 5U / g , enzyme reaction temperature 30°C, 1mol / L phosphate buffered saline with a pH of 7.0 and 10mL reaction time in 8h, the product after the reaction was treated by a nanofiltration membrane with a molecular weight cut-off of 350Da, before and after enzyme treatment and after nanofiltration treatment, single The contents of sugars, disaccharides and fructooligosaccharides are shown in Table 1.

[0023] Table 1 The effect before and after primary fructo-oligosaccharide enzyme treatment and nanofiltration treatment

[0024]

Embodiment 2

[0026] Take 100mL of primary fructooligosaccharides with a purity of 80% (sucrose content is 0.1mol / L) in an enzyme reactor, glucosyltransferase 10U / g, enzyme reaction temperature 30°C, 1mol / L, pH 7.0 phosphate buffered salt 10mL , and the samples with reaction time between 1 and 20 hours were taken for analysis, and the results are shown in Table 2.

[0027] The impact of table 2 different reaction times on primary fructo-oligosaccharide enzyme enzyme treatment

[0028]

Embodiment 3

[0030] Take 100mL of primary fructooligosaccharides with a purity of 80% (sucrose content is 0.1mol / L) in an enzyme reactor, glucosyltransferase 1 ~ 10U / g, enzyme reaction temperature 30 ° C, 1mol / L, pH 7.0 phosphate buffer Salt 10mL, reaction time 8h, the results are shown in Table 3.

[0031] Table 3 Effects of different enzyme additions on primary fructooligosaccharide enzyme treatment

[0032]

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Abstract

The invention discloses a method for purifying fructose oligosaccharide. The method comprises the steps of performing enzymolysis on inulin by using inulase to obtain a primary fructose oligosaccharide mixed solution, putting the primary fructose oligosaccharide mixed solution into a reactor, adding a phosphate buffer of which the pH is 5.0-8.0 and a glucosyltransferase at 10-40 DEG C, performing enzymolysis for 1-20h, treating the enzymolysis solution through a nano-filtration membrane, and collecting the trapped solution to obtain a fructose oligosaccharide solution of which the purity is more than 98%. By adopting the method, saccharose is decomposed into monosaccharide, the monosaccharide is further removed through the nano-filtration membrane, and fructan of which the polymerization degree is more than 2, so that the purity of the fructose oligosaccharide can be greatly improved and can exceed 98%. Meanwhile, after the saccharose is decomposed into the monosaccharide, the saccharide in the nano-filtration permeate is monosaccharide which can be directly utilized by microbes and is applied in the fermentation industry as a byproduct, so that the market demand is huge.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and relates to a method for purifying fructooligosaccharides, in particular to a method for reducing the content of disaccharides in products after inulinase enzymatic hydrolysis of inulin and improving the purity of fructooligosaccharides. Background technique [0002] Inulin, also known as inulin, as one of the energy storage methods of plants, is widely distributed in nature, and its content can be found in more than 30,000 kinds of plants to store their energy. Inulin is a linear straight-chain polysaccharide made of fructose connected by β(2-1) bonds, often with a glucose at the end, and the degree of polymerization DP is usually between 2 and 60. In fact, inulin is also a variety of different degrees of polymerization. A mixture of fructans. Fructans with a lower degree of polymerization (DP=2-9) are usually called fructo-oligosaccharides, fructans with a degree of polymerizat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H3/06C07H1/06
Inventor 马江锋姜岷韦策吕浩张敏李干禄吴昊
Owner 完美(广东)日用品有限公司
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