734 results about "Monosaccharide" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium longum subsp. Longum as well as a composition, a pharmaceutical composition, a culture, a food product or a dietary supplement containing the bifidobacterium longum subsp. Longum and application of the bifidobacterium longum subsp. Longum. The bifidobacterium longum is preserved in the Guangdong Microbial Culture Collection Center on January 21, 2025, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.65836. The bifidobacterium longum subsp. Longum has relatively strong gastric acid and cholate tolerance and intestinal colonization capability; the bifidobacterium longum subsp. Longum can effectively utilize bifidobacterium bifidum to release monosaccharide or disaccharide outside cells for efficient proliferation, and the composition containing the bifidobacterium longum subsp. Longum, the bifidobacterium bifidum and breast milk oligosaccharide can release a large amount of short-chain fatty acid, so that the effects of maintaining the physiological function of an organism and improving the health of the organism are achieved.

The invention relates to gardenia polysaccharide as well as a preparation method and application thereof. The gardenia polysaccharide comprises the following monosaccharide units: rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, or mainly comprises rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose; the molar ratio of rhamnose to glucuronic acid to galacturonic acid to glucose to galactose to arabinose in the gardenia polysaccharide is (3-15): (1-8): (4-18): (1-6): (15-35): (25-70). According to the gardenia polysaccharide, FFA induced mouse primary parenchymal hepatic cell lipid accumulation can be reduced, no obvious cytotoxic effect exists, CDAHFD diet induced hepatic fibrosis can be reduced, and the gardenia polysaccharide has a good effect of preventing and / or treating non-alcoholic steatohepatitis.

The invention discloses a kind of eucommia leaf fermentation extract and its preparation method and application, and belongs to the technical field of microorganisms and feed additives. With eucommia leaf and wheat bran as substrates, by configuring a specific black aspergillus niger-trichoderma harzianum-saccharomyces cerevisiae complex bacterial liquid as a fermentation agent, a closed fermentation is carried out to make black aspergillus, trichoderma harzianum, and saccharomyces cerevisiae ferment to produce a large amount of cellulase, protease, lipase, amylase and glucosidase, etc., and then by pumping and circulating treatment, the cellulose, protein, starch, beta-glycan and other macromolecular substances in eucommia leaf are further enzymolyzed into protein oligopeptides, monosaccharides, and soluble oligosaccharides that are beneficial to livestock and poultry absorption. The eucommia leaf fermentation extract prepared by the present invention is used as a feed additive for beef cattle feeding, improves the daily weight gain and feed intake of beef cattle, and significantly reduces the feed-to-meat ratio, saves feed consumption, improves the protein content in meat, reduces the fat content, reduces energy, and improves meat quality.

The invention discloses hawthorn leaf pectic polysaccharide with lipid-lowering activity and a preparation method and application thereof, and belongs to a preparation method of plant polysaccharide, the preparation method comprises the following steps: refluxing hawthorn leaves with ethanol, discarding monosaccharide and small molecule compounds, refluxing with hot water to extract medicinal materials, removing protein through an enzymolysis method and a Sevage reagent method, decolorizing with polyamide resin, and drying to obtain the hawthorn leaf pectic polysaccharide with lipid-lowering activity. The crude polysaccharide is separated through DEAE-52 column chromatography, and finally, the crude polysaccharide is further purified through Sephadex G-100, so that the uniform purified polysaccharide is obtained. Tests on pancreatic lipase inhibition, taurocholate binding capacity and glycocholate binding rate show that the compound has remarkable lipid-lowering activity and is expected to become a potential natural lipid-lowering preparation.

The invention relates to the technical field of tea watering, and discloses a tea watering device which comprises a processing box, the back of the processing box is fixedly connected with a water tank, the top of the water tank is communicated with a plurality of spray pipes, the spray pipes extend into the processing box, and inverted-T-shaped sliding grooves are formed in the left side and the right side of the processing box respectively. Reciprocating swinging of a rectangular shaft can drive a spraying pipe to swing, reciprocating swinging of a spraying head can expand the spraying coverage range, water is more evenly distributed on the surfaces of tea leaves, left-right movement of a moving sleeve can drive the spraying pipe to move left and right, and therefore the spraying range of the tea leaves is widened, and the tea leaves can make full contact with the water; after the tea leaves are in full contact with water, hydrolase can be activated by full contact with the water, macromolecular substances such as protein and polysaccharide in the tea leaves can be decomposed into micromolecular substances such as amino acid and monosaccharide by the hydrolase, and the taste and aroma components of the tea leaves are increased.

The invention relates to a particulate composition comprising, or substantially consisting of, urea, an inhibitor, preferably a urease inhibitor and / or a nitrification inhibitor, and an additive, wherein the additive comprises components (i), (ii), and optionally (iii): (i) one or more polyhydric alcohols; preferably one or more carbohydrates, preferably selected from monosaccharides, disaccharides, and oligosaccharides; (ii) a first polymer, preferably an amine-containing polymer; more preferably a polyvinylamine or a polyalkyleneimine; and (ii) optionally, a second polymer, preferably a polar polymer; more preferably a water-soluble polar polymer. The invention further relates to a process for producing the particulate composition, an additive for a particulate composition, and the use of the particulate composition as a fertilizer.

The invention relates to the technical field of microbial fermentation feeds, in particular to a method for producing a protein feed through solid-state fermentation of distiller's grains based on Trichoderma harzianum pretreatment. According to the method, the vinasse is pretreated by the crude enzyme prepared from trichoderma harzianum, and the formula and fermentation conditions of an enzyme production culture medium are optimized, so that the filter paper enzyme activity of cellulase is remarkably improved. By adding the surfactant into a fermentation system and optimizing the variety of the surfactant, the yield of the fermented monosaccharide is improved, and the problem of musty taste in a traditional fermented product is solved while the degradation efficiency of lignocellulose is improved. Furthermore, a synchronous saccharification and fermentation process is adopted, vinasse is fermented through compounded saccharomycetes and crude enzyme liquid, the trichoderma harzianum fermented protein feed is prepared and obtained, digestion and absorption of animals are easier, lactic acid fermentation is conducted on the trichoderma harzianum fermented protein feed again through lactobacillus fermentum, the flavor of the feed is improved, the bitter taste of the feed is reduced, and the sour taste is improved. The method realizes efficient utilization of vinasse resources.

A semi-carbonized carbonized material is produced from the raw wood material to be treated, a highly energy semi-carbonized carbonized material is produced from the raw wood material to be treated, and a highly energy wood biogas is produced from the highly energy semi-carbonized carbonized material. [Solution] The system includes a subcritical water reaction treatment device, a wood biopellet generation device, and a wood biogasification furnace. The subcritical water reaction treatment device forms a semi-carbonized wood biomaterial consisting mainly of low molecular weight cellulose components and low molecular weight lignin components from wood material, and multiple hemicellulose-based monosaccharides that are solidified and fixed to these cellulose components and lignin components. The wood biopellet generation device produces wood biopellets from the semi-carbonized wood biomaterial, and the wood biogasification furnace produces wood biogas from the wood biopellets.

The invention discloses a functional feed formula for improving endurance and reproductive performance of donkeys, and relates to the technical field of feed formulae, the functional feed formula comprises selection and pretreatment of raw medicinal materials, nutrient element proportioning, treatment of functional reinforcing materials and treatment after molding, the functional reinforcing materials comprise a functional formula A and a functional formula B, the method has the advantages that the mixed leaves are subjected to low-temperature freeze-drying treatment and then crushed into powder, bioactive components such as protein and flavonoids in the leaves are reserved, the leaves can be used as a high-quality plant protein source and a functional component source, carbon dioxide gas is introduced into the compound protein leaf powder for activation, and the compound protein leaf powder can be used as a high-quality plant protein source and a functional component source. The method has the advantages that bioactive components in the leaf powder can be activated, the bioavailability of the leaf powder can be increased, polysaccharide in the high-sugar Chinese velvet herb can be further decomposed into monosaccharide and oligosaccharide by saccharifying the high-sugar Chinese velvet herb, and the energy release speed can be increased.

The invention discloses a method for preparing multi-branch oligosaccharide. According to the method, an in-vitro enzymatic synthesis strategy is adopted, monosaccharide activation and glycosyl transferase catalytic reaction are coupled, operation is simple and safe, the reaction conversion rate and the product yield are greatly improved, and a foundation is laid for industrial production of multi-branched oligosaccharides, especially multi-branched human milk oligosaccharides. According to the invention, synthesis of human milk oligosaccharides with three-branch, four-branch and other multi-branch structures can be realized, and reference is provided for synthesis of oligosaccharides and glycoconjugates with more complex structures.

The invention provides kelp fucoidin with low molecular weight and high anti-anaphylaxis as well as a preparation method and application thereof, and belongs to the technical field of food processing. The preparation method comprises the following steps: mixing kelp fucoidin with water to obtain a kelp fucoidin solution; and mixing the kelp fucoidin solution with H2O2 and Vc, reacting, adjusting the pH value, dialyzing and freeze-drying to obtain the kelp fucoidin with low molecular weight and high anti-allergic property. The H2O2-Vc degradation system is innovatively applied to preparation of the low-molecular-weight kelp fucoidin and improvement of the anti-allergic activity of the low-molecular-weight kelp fucoidin. In the degradation process, the chemical composition of the fucoidin is partially changed, but monosaccharide types and functional groups are not influenced. Through multiple ways of cell model and animal model experiments, the degraded laminaria fucoidin is more prominent in anti-allergic activity. Technical support is provided for development of seaweed-derived anti-allergic functional active substances, and high-valued utilization of kelp resources in the fields of functional food and medicines is further promoted.

The invention discloses a coumarin tyramine glycoside compound and application thereof in preparation of PTP1B (protein tyrosine phosphatase 1B) inhibitors and antidiabetic drugs. The structural formula of the compound is # imgabs0 #, in the formula, R represents fat (aromatic) acyl or monosaccharide substituent, and R'represents hydrogen atom or acetyl; 4-methoxycoumarin 4 '-acetyl-O-L-rhamnose is used as an initial raw material, fat (aromatic) acyl or monosaccharide substituent groups are introduced into hydroxyl at the second position and hydroxyl at the third position of L-rhamnose at the same time, and then protecting groups of glycosyl are removed, so that the L-rhamnose is prepared. Pharmacological activity tests show that the compounds have good inhibitory activity on protein tyrosine phosphatase 1B, which indicates that the coumarin tyramine glycoside compounds can be researched and utilized as novel PTP1B inhibitors and antidiabetic drugs.

The invention discloses a method for determining the content of monosaccharide in seaweed, and belongs to the technical field of biological detection. According to the method, an ion chromatography sugar separation system is adopted, a pulse ampere detector is combined, CarboPac PA20 is used as a sugar analysis column, and the eight monosaccharide components in the seaweed can be detected under the limited extraction and purification method. The method is low in detection limit, good in stability and suitable for measuring the content of free monosaccharide and polysaccharide hydrolyzed monosaccharide in common algae (such as brown algae, green algae and red algae).

Provided are a novel sodium alginate lyase AlyB, a preparation method therefor and the use thereof. The main cracking product of the novel sodium alginate lyase AlyB is a monosaccharide, which has the effects of resisting oxidation, resisting viruses and inhibiting an angiotensin converting enzyme, and thus can be applied to the fields of cosmetics, medicines, healthcare foods and the like.

The invention discloses a method for improving the quality of phyllanthus emblica fruit juice through an enzymolysis-assisted pulsed electric field, which comprises the following steps: firstly, carrying out preliminary enzymolysis treatment on phyllanthus emblica fruits through a low-concentration compound enzyme solution to form micropores in the surfaces of cell walls; then, the pore diameter of the preliminarily formed micropore area is further expanded under the action of a pulsed electric field, the cell structure is destroyed to form a pore channel, through combined regulation and control of enzymolysis and electric field parameters, efficient release of micromolecular nutritional ingredients such as vitamin C, polyphenol substances, total flavonoids and monosaccharide is achieved through the synergistic effect of the enzymolysis and the electric field parameters, and the effect of improving the nutrient content is achieved. Meanwhile, the excessive precipitation of macromolecular polysaccharide is limited, the taste of the fruit juice is improved, the clarity of the fruit juice and the stability of nutritional ingredients are ensured, the consistency of the taste of the product is maintained, in addition, the enzyme dosage is effectively reduced, the safety problem caused by enzyme residues is reduced, the enzymolysis time and the pulse intensity are also remarkably reduced, and the production cost is reduced; the method meets the requirement of green production and is suitable for industrial large-scale application.

The present invention provides a chemical synthesis method of EPS-G7, wherein β-cyclodextrin A is acetylated to obtain β-acetylcyclodextrin B, B is ring-opened in a mixed solution of acetic anhydride / concentrated sulfuric acid to obtain a chain acetylated heptose C, p-nitrophenol is condensed with intermediate C using boron trifluoride acetonitrile to obtain a key intermediate p-nitrophenyl (G1)-O-acetyl-D-maltoseptaglycoside D with α as the dominant configuration, D is removed from the acetyl group under the action of sodium methoxide to obtain pNP-G7 (p-nitrophenyl (G1)-D-maltoseptaglycoside), pNP-G7 is in the presence of p-toluenesulfonic acid and dimethoxyethane, and acetal protection is formed between the hydroxyl groups on C4 and C6 of the terminal monosaccharide to obtain EPS-G7. The reaction raw materials used in the method of the present invention are cheap and readily available, and the chemical synthesis of α-amylase detection reagent EPS-G7 is realized, which is a simple, economical and efficient synthesis method.

The invention provides liquid deamidase compositions comprising mono- or disaccharide(s), which exhibit high enzymatic stability after storage.

Disclosed in the present invention is a process for preparing a monosaccharide solution by means of acid gradient treatment of a straw lignocellulose. The process comprises the steps of: S1: performing a first acidolysis reaction on a lignocellulose biomass using an acid solution with a concentration of 50 wt% or above to obtain a first acidolysis solution; S2: uniformly mixing the first acidolysis solution with an organic solvent, and performing solid-liquid separation to obtain a glycan-containing residue; and S3: adding water and / or an acid solution to the glycan-containing residue to obtain a mixed solution with an acid concentration of 45 wt% or below, and performing a second acidolysis reaction to obtain a monosaccharide-containing reaction solution. In the present invention, pretreatment is performed at room temperature and atmospheric pressure without the need to provide additional energy, and various agents used in the pretreatment process are all recoverable, which greatly reduces the production cost. In addition, in the present invention, a relatively low liquid-solid ratio is used, monosaccharide can be directly obtained after the pretreatment, and finally a monosaccharide solution with a high and adjustable concentration is obtained, thereby avoiding a large amount of input in enzyme costs.

The invention belongs to the technical field of saccharide separation, and relates to a high-selectivity spiral-wound membrane and application thereof. A separation membrane of a membrane bag contained in a membrane group of the high-selectivity spiral-wound membrane is formed by a polyamide separation membrane, and the polyamide separation membrane comprises a porous supporting layer and a modified polyamide separation layer loaded on the surface of the porous supporting layer; the modified polyamide separation layer is formed by interfacial polymerization of composite polyamine and polyacyl chloride in the presence of humate, and the composite polyamine is a compound of aliphatic polyamine and adamantane modified diamine according to a mass ratio of 1: (0.1-0.5). The adamantane modified diamine is a product obtained by carrying out addition reaction on adamantane diamine and alicyclic diisocyanate according to a molar ratio of (1.2-2): 1; the general formula of the adamantane diamine is NH2 (CH2) nR1 (CH2) mNH2. The spiral-wound membrane provided by the invention can stably operate at relatively high flux under a high-pressure condition, and can be stably used for separating monosaccharide and polysaccharide for a long time.

The invention discloses a radix pseudostellariae extract as well as a preparation method and application thereof. The preparation method comprises the following steps: providing a radix pseudostellariae raw material; the radix pseudostellariae raw material is subjected to water extraction to prepare a water extract; eluting the water extract through macroporous adsorption resin column chromatography to prepare a first extract; and eluting the first extract through ion exchange resin column chromatography to prepare the radix pseudostellariae extract. By adopting a method of combining macroporous adsorption resin column chromatography and ion exchange resin column chromatography, the yield of the radix pseudostellariae extract is improved, particularly the polysaccharide content and the specific monosaccharide content and proportion in the radix pseudostellariae extract are improved, and the functional activity of the radix pseudostellariae extract is greatly improved. Meanwhile, the preparation process greatly reduces the use of organic solvents, is green and safe in production process, and has good use value in the fields of food, medicines and the like.

The invention provides a lactic acid bacteria exopolysaccharide and a preparation method thereof, and the preparation method comprises the following steps: firstly, optimizing culture medium components and culture conditions to respectively obtain separately-cultured lactic acid bacteria and yeast seed solutions, then mixing the lactic acid bacteria and yeast seed solutions for co-culture, and further optimizing the culture medium components and the culture conditions to obtain an exopolysaccharide culture solution. The yield of the exopolysaccharide obtained by the preparation method disclosed by the invention is greatly improved compared with that of the exopolysaccharide obtained by independently culturing lactobacillus, and the content can be up to 90g / L. The invention belongs to the technical field of microbial co-culture technology and production and application of probiotic secondary metabolites. Lactobacillus plantarum and Pseudomonas stutzeri are co-cultured, expression of quorum sensing related genes of lactobacillus plantarum is up-regulated, biomass is increased, expression of genes of dextran sucrase which is a key enzyme for synthesizing exopolysaccharides is up-regulated, activity of dextran sucrase is improved, and yield of exopolysaccharides is increased. The co-culture extracellular polysaccharide is homopolysaccharide with glucose as monosaccharide, and compared with single culture extracellular polysaccharide and commercial glucan, the co-culture extracellular polysaccharide has good oxidation resistance, emulsification, heavy metal adsorption and probiotic proliferation promoting capacity, and is expected to be widely applied to the fields of food, medicine, environmental protection and the like.

The invention discloses a raspberry homogeneous polysaccharide. The monosaccharide components of the raspberry homogeneous polysaccharide comprise fucose, rhamnose, arabinose, galactose, xylose, galacturonic acid, glucuronic acid and apiose. The main chain structure of the raspberry homogeneous polysaccharide is 1, 4-linked galacturonic acid pyranose, and the weight-average molecular weight of the raspberry homogeneous polysaccharide is 8.54 + / -5.0 kDa. The invention discloses a raspberry homogeneous polysaccharide component for inhibiting dietary oil absorption and preventing and improving obesity, which is prepared by the steps of water extraction and alcohol precipitation, ion exchange chromatographic column elution and permeation gel chromatographic column elution. The structure of the compound is analyzed by high performance gel permeation chromatography, infrared spectroscopy, ion chromatography, gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. The homogeneous polysaccharide component can be used for improving obesity and fat tissue accumulation hypertrophy, and the efficacy of the homogeneous polysaccharide component is related to inhibition of absorption of dietary oil in the intestinal tract and promotion of discharge of the dietary oil into the body.

The invention is suitable for the technical field of chemical synthesis, and provides a nitrogen modified metal composite material and a preparation method and application thereof. According to the method, primary biomass and derivatives thereof are carbonized in ethylene glycol containing a nitrogen precursor, meanwhile, bimetal is reduced and loaded on a nitrogen modified functional carbon carrier, so that an alloy catalytic site is formed, and the alloy catalytic site is beneficial to adsorption of monosaccharide, promotion of mass transfer of substances and promotion of monosaccharide oxidation into a final product saccharic acid; therefore, the monosaccharide oxidation reaction is efficiently carried out, and a theoretical basis is provided for directly synthesizing saccharic acid from agricultural wastes such as straw through chemical catalytic oxidation. The monosaccharide comprises xylose, glucose and the like; the product saccharic acid comprises xylonic acid, gluconic acid, glucaric acid and the like. Results show that the conversion rate of glucose is 100%, the yield of gluconic acid is 81.7%, and the yield of glucaric acid is 13.1%.

The invention discloses an innovative process for preparing a liquid fertilizer from an acetylisovaleryltylosin tartrate waste liquid for vegetable planting, and relates to the technical field of fertilizer preparation, and the innovative process comprises the following specific steps: step 1, preliminary pretreatment of the acetylisovaleryltylosin tartrate waste liquid; the preparation method has the advantages that the aminopropyltriethoxysilane enhances the dispersion stability of titanium dioxide in a waste liquid environment, the reduction of the effective surface area capable of participating in the reaction due to agglomeration is avoided, and the comprehensive and efficient promotion of the photocatalytic reaction is guaranteed; amino functional groups carried by aminopropyltriethoxysilane can be specifically combined with various organic substances in the waste liquid to guide a photocatalytic reaction to directionally act on specific organic macromolecules, so that the decomposition process is more targeted and efficient, the conversion efficiency of nutritional ingredients is greatly improved through the synergistic effect, and the photocatalytic effect of the waste liquid is improved. Macromolecular protein, polysaccharide and the like in the waste liquid are efficiently converted into micromolecular nutrients such as amino acid, monosaccharide and the like, and the content of effective nutritional ingredients in the liquid fertilizer is remarkably increased.

The present invention discloses roxburghii polysaccharide and its preparation method and application. Wherein roxburghii crude polysaccharide is purified to obtain two polysaccharides RP1 and RP3, RP1 monosaccharide types include galacturonic acid, galactose, rhamnose and arabinose, and its molar ratio is 1.00:3.45:1.88:1.82, RP3 monosaccharide types include galactose, rhamnose and arabinose, galacturonic acid, galactose, rhamnose and arabinose, and its molar ratio is 1.00:0.37:0.37:0.25. It has been confirmed by in vitro experiments that the above-mentioned roxburghii polysaccharide has the ability to significantly inhibit the proliferation of MCF-7 breast cancer cells, as well as the effect of inducing apoptosis of MCF-7 breast cancer cells, and can be developed to prepare breast cancer therapeutic drugs; At the same time, the above-mentioned roxburghii polysaccharide has no effect on normal L929 cells. Therefore, the above-mentioned roxburghii polysaccharide has broad application prospects and has good social and economic benefits.

The invention discloses a method for improving moso bamboo cellulase hydrolysis conversion efficiency through amino acid-based deep eutectic solvent pretreatment, and belongs to the technical field of biomass recycling. The method comprises the following steps: mixing an L-cysteine solid and a formic acid solution, performing water bath heating to prepare an acidic eutectic solvent, performing microwave-assisted eutectic solvent pretreatment on moso bamboos, and performing filtration separation and enzymolysis; wherein the molar ratio of the L-cysteine to the formic acid is 1: (4-5). A deep eutectic solvent formed by formic acid and cysteine has a strong hydrogen bond network and an acid environment, beta-O-4 ether bonds in lignin are promoted to be broken, sulfydryl of cysteine participates in nucleophilic attack, a lignin-carbohydrate complex is further destroyed, formic acid can hydrolyze acetyl and glucosidic bonds in hemicellulose, and the lignin-carbohydrate complex is further destroyed. And degrading into an oligomer or monosaccharide. The phyllostachys pubescens components are efficiently separated, the cellulose enzymolysis saccharification capacity is improved, and effective utilization of all the phyllostachys pubescens components is facilitated.

The invention is applicable to the technical field of biology, and provides a method for rapidly extracting plant rhizome extracellular vesicles from monosaccharide and compound enzyme. According to the method, the plant extracellular vesicles are extracted by an enzymolysis-monosaccharide solution extraction coupling method for the first time, and the defect that an extraction method is single is overcome. According to the method, the conditions are mild, the primary property, the integrity and the biological activity of the vesicle structure are kept, the extraction purity is high, and the particle size distribution is uniform and concentrated in the range of 20-300 nm. The total protein extraction amounts of glucose, galactose and fructose solutions are respectively increased by 36.09% + / -1.18%, 37.65% + / -2.83% and 36.83% + / -2.59% compared with those of PBS (Phosphate Buffer Solution) extraction treatment. In addition, the'single ultracentrifugation 'purification process overcomes the problem of time consumption of traditional gradient centrifugation, and the time is shortened by more than 3h compared with that of a traditional extraction method. The method breaks through the technical bottleneck of large-scale production of the plant extracellular vesicles, and has great application and popularization values.

The invention discloses an opencast coal mine truck transportation road dust suppressant for a high-temperature dry environment and a preparation method of the opencast coal mine truck transportation road dust suppressant. The dust suppressant is prepared by mixing 20-30% by mass of glucose, 0.05-0.15% by mass of a penetrant, 1.5-2.5% by mass of a water-retaining agent, 1.5-2.5% by mass of a moisture absorbent and the balance of water. The dust suppressant is an environment-friendly material, the main material glucose is a monosaccharide which is most widely distributed and most important in nature, and the dust suppressant has the characteristics of being non-toxic, odorless, green, environment-friendly, wide in source and application and the like. The dust suppressant integrates the functions of wetting, moisture absorption, water retention and the like, can keep dust on a road surface transported by a large mine truck of an open pit coal mine wet and effectively suppress flying dust on the road surface, is simple in preparation process, low in material cost, green and environment-friendly in material and wide in material source, and has good flowability and feasibility. The dust suppressant is applied to the open pit coal mine end slope transportation road, the environment quality and the traffic safety of the open pit coal mine can be effectively guaranteed, and the manpower and material resource investment of environment maintenance is reduced.

The present invention provides methods for glycosylation and compound preparation. The compounds include galactosylated, sialylated, fucosylated, and N-acetylglucosaminated compounds from simple oligosaccharides and sugars of animal, plant, or microbial origin. In certain embodiments, the invention provides for trinucleotide-free enzymatic production of oligosaccharides starting from simple sugars, including plant-based sugars. The present invention also provides methods of enzymatic production of fucosylated oligosaccharides and fucosylated antibody-glycan conjugates from ordinary enzymes. The production may be a cell-free, one-pot synthesis using an enzyme and, in some embodiments, an immobilized enzyme. The synthesis is a highly customizable and efficient cell-free manufacturing process. In some embodiments, lactose derivatives and human milk oligosaccharides (HMOs) are produced.

The present invention relates to antibody conjugates which are particularly suitable for targeting cells expressing PTK7, in particular tumor cells. An antibody conjugate according to the present invention has structure (1): AB-[(L6) b-{Z-L-D} x] y (1) wherein AB is an antibody capable of targeting a tumor expressing PTK7; l is a joint connecting Z to D; z is a linking group; l6 is-GlcNAc (Fuc) w-(G) j-S-(L7) w '-wherein G is a monosaccharide, j is an integer in the range of 0 to 10, S is a sugar or a sugar derivative, GlcNAc is N-acetylglucosamine, and Fuc is fucose, w is 0 or 1, w' is 0, 1 or 2, and L7 is-N (H) C (O) CH2-,-N (H) C (O) CF2-or-CH2-; d is selected from the group consisting of an anthracycline, a camptothecin, a tubulysin, an endiyne, an amanitine, a duocarmycin, a maytansinoid, an aurestatin, eribulin, a BCL-XL inhibitor, a miscanthus semiaquilaria, a KSP inhibitor, a TLR agonist, an indolo-benzodiazepine dimer or a pyrrolo-benzodiazepine dimer (PBD), and an analog or prodrug thereof; b is 0 or 1; x is 1 or 2; and y is 1, 2, 3 or 4. The invention further relates to a method for preparing said antibody conjugate of structure (1) and to the use of said antibody conjugate of structure (1).