The invention is applicable to the technical field of
biology, and provides a method for rapidly extracting
plant rhizome extracellular vesicles from
monosaccharide and compound
enzyme. According to the method, the
plant extracellular vesicles are extracted by an enzymolysis-
monosaccharide solution extraction
coupling method for the first time, and the defect that an extraction method is single is overcome. According to the method, the conditions are mild, the primary property, the integrity and the
biological activity of the
vesicle structure are kept, the extraction purity is high, and the
particle size distribution is uniform and concentrated in the range of 20-300 nm. The
total protein extraction amounts of glucose,
galactose and
fructose solutions are respectively increased by 36.09% + / -1.18%, 37.65% + / -2.83% and 36.83% + / -2.59% compared with those of PBS (
Phosphate Buffer Solution) extraction treatment. In addition, the'single ultracentrifugation 'purification process overcomes the
problem of time consumption of traditional
gradient centrifugation, and the time is shortened by more than 3h compared with that of a traditional extraction method. The method breaks through the technical
bottleneck of large-scale production of the
plant extracellular vesicles, and has great application and popularization values.