9 results about "Ketose" patented technology

Various processes for preparing C4 aldoses and / or ketones thereof are described. Various processes are described for preparing C4 aldoses and / or ketones thereof from feed compositions comprising glycolaldehyde. Also, various processes for preparing useful downstream products and intermediates, such as erythritol and erythronic acid, from the C4 aldoses and / or ketones thereof are described.

The invention relates to the technical field of biology, in particular to a hexanose-2-epimerase mutant and application thereof. According to the hexaldehyde aldose-2-epimerase mutant and the application of the hexaldehyde aldose-2-epimerase mutant in the embodiment of the invention, the hexaldehyde aldose-2-epimerase mutant generates mutation beneficial to improvement of hexaldehyde aldose-2 hydroxyl isomerization catalytic activity at at least one key site in an amino acid sequence as shown in SEQ ID NO: 19; through the mode, the hexaldehyde sacchar-2-epimerase mutant shows excellent hexaldehyde sugar C-2 hydroxyl isomerization catalytic activity, can efficiently convert D-glucose into D-mannose, and is beneficial to improving the synthesis efficiency of the D-mannose.

Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

The application discloses application of 1-chloro-4-hydrazinyl phthalazine as a reactive matrix in monosaccharide MALDI MSI in-situ analysis. The monosaccharide includes glucose, fructose, fucose and arabinose. The application develops 1-chloro-4-hydrazinyl phthalazine (CHP) as a reactive matrix, which is used for derivatization of monosaccharide in biological tissues and in-situ differentiation of monosaccharide isomers. Taking carrot as a model, monosaccharide on the carrot tissue is derivatized, and a MALDI-MSI experiment is carried out. It is worth noting that characteristic fragments generated by secondary mass spectrometry of the derivatization product can be used for further distinguishing aldoses and ketoses, thereby differentiating monosaccharide isomers and revealing different spatial distributions of monosaccharide isomers in organisms.

The application discloses application of a phenylpropionic acid compound in detection of monosaccharide composition of traditional Chinese medicine polysaccharide. The phenylpropionic acid compound has a structure as shown in formula (I). The application provides a new application of the phenylpropionic acid compound, and preliminary realization of quality control research on traditional Chinese medicine preknowing polysaccharide. Compared with a method for detecting monosaccharide composition of traditional Chinese medicine polysaccharide by using 1-phenyl-3-methyl-5-pyrazolone as a pre-column derivatization reagent, the application has the following advantages: 1. Derivatization of aldehyde sugar and ketose can be simultaneously realized; 2. The reaction temperature is more moderate, and the safety of the reaction is greatly improved; 3. The detection wavelength of HPLC-UV is improved from 245 nm to 274 nm, interference is reduced, and sensitivity is improved; and 4. The pre-column derivatization reagent is low in cost and easy to obtain.

The application discloses a surface treatment agent for a lithium ion battery positive electrode material and application thereof. The surface treatment agent for the lithium ion battery positive electrode material comprises monosaccharide or oligosaccharide. The monosaccharide comprises one or more of aldehyde sugar and ketose; the oligosaccharide comprises one or more of lactose, fructooligosaccharide, maltose, cellobiose, trehalose, raffinose, melezitose and gentiobiose. The surface treatment agent forms strong binding force with transition metals on the surface of the positive electrode material, weakens the solvation of ester solvents in electrolyte to the surface transition metals, prevents the surface transition metals from dissolving in the electrolyte and depositing to the negative electrode side. Meanwhile, the surface transition metals contained in the process weaken and inhibit the catalytic decomposition process of the electrolyte, thus the decomposition of the electrolyte on the surface of the high-voltage material is weakened and inhibited, the deposition of electrolyte decomposition products on the positive electrode side is reduced, and the cycle of the battery is more stable.

To provide integrated processes for catalytic conversion of carbohydrate to ethylene glycol and / or propylene glycol using a homogeneous, tungsten-containing retro-aldol catalyst.SOLUTION: A carbohydrate is subjected to retro-aldol conversion and hydrogenation. Ethylene glycol and propylene glycol are separated from the reaction product for purification, and at least a portion of the remaining fraction is subjected to ion exclusion chromatography to provide an eluant containing tungsten species and a subsequent eluant containing organic acids and a substantially reduced concentration of tungsten species. At least a portion of the eluant containing tungsten species can be recycled for reuse directly or with intervening unit operations to enhance the catalytic activity of the tungsten species. The organic-containing fraction can be subjected to unit operations to provide salable products or subjected to selective hydrogenolysis to lower glycols.SELECTED DRAWING: Figure 1

Integrated processes are disclosed for the catalytic conversion of carbohydrate to ethylene glycol and / or propylene glycol using a homogeneous, tungsten-containing retro-aldol catalyst. In these processes, the carbohydrate is subjected to retro-aldol conversion and hydrogenation to provide a reaction product containing ethylene glycol and / or propylene glycol, other reaction process including organic acids, itols and tungsten species. Ethylene glycol and propylene glycol are separated from the reaction product for purification, and at least a portion of the remaining fraction is subjected to ion exclusion chromatography to provide an eluant containing tungsten species and a subsequent eluant containing organic acids and a substantially reduced concentration of tungsten species. At least a portion of the eluant containing tungsten species can be recycled for reuse directly or with intervening unit operations to enhance the catalytic activity of the tungsten species. The organic-containing fraction can be subjected to one or more unit operations to provide salable products or subjected to selective hydrogenolysis to lower glycols.