20 results about "Ketose" patented technology

Integrated processes are disclosed for the catalytic conversion of carbohydrate to ethylene glycol and / or propylene glycol using a homogeneous, tungsten-containing retro-aldol catalyst. In these processes, the carbohydrate is subjected to retro-aldol conversion and hydrogenation to provide a reaction product containing ethylene glycol and / or propylene glycol, other reaction process including organic acids, itols and tungsten species. Ethylene glycol and propylene glycol are separated from the reaction product for purification, and at least a portion of the remaining fraction is subjected to ion exclusion chromatography to provide an eluant containing tungsten species and a subsequent eluant containing organic acids and a substantially reduced concentration of tungsten species. At least a portion of the eluant containing tungsten species can be recycled for reuse directly or with intervening unit operations to enhance the catalytic activity of the tungsten species. The organic-containing fraction can be subjected to one or more unit operations to provide salable products or subjected to selective hydrogenolysis to lower glycols.

Sedoheptulose, which is a saccharide falling within the categories of ketoses and heptuloses, is one of a small number of heptuloses occurring in nature. Provided are a method for producing sedoheptulose using a bacterium owing to the deletion or attenuation of a specific enzymatic function, a method for improving the productivity of sedoheptulose by the bacterium, and the bacterium.

Various processes for preparing C4 aldoses and / or ketones thereof are described. Various processes are described for preparing C4 aldoses and / or ketones thereof from feed compositions comprising glycolaldehyde. Also, various processes for preparing useful downstream products and intermediates, such as erythritol and erythronic acid, from the C4 aldoses and / or ketones thereof are described.

The invention relates to the technical field of biology, in particular to a hexanose-2-epimerase mutant and application thereof. According to the hexaldehyde aldose-2-epimerase mutant and the application of the hexaldehyde aldose-2-epimerase mutant in the embodiment of the invention, the hexaldehyde aldose-2-epimerase mutant generates mutation beneficial to improvement of hexaldehyde aldose-2 hydroxyl isomerization catalytic activity at at least one key site in an amino acid sequence as shown in SEQ ID NO: 19; through the mode, the hexaldehyde sacchar-2-epimerase mutant shows excellent hexaldehyde sugar C-2 hydroxyl isomerization catalytic activity, can efficiently convert D-glucose into D-mannose, and is beneficial to improving the synthesis efficiency of the D-mannose.

This invention discloses a method for preparing an immobilized ketose 3-epimerase and its application, belonging to the field of immobilized enzyme preparation technology. The invention comprises the following steps: first, pretreatment of anion exchange resin; second, adsorption of the protease onto the ion exchange resin; and third, cross-linking with glutaraldehyde. The immobilized enzyme prepared by this invention is simple to prepare, inexpensive, and requires simple and mild reaction conditions. Immobilization improves the relative activity and stability of the enzyme, and the prepared immobilized enzyme is firmly bound and can undergo multiple enzyme-catalyzed reactions. This discovery has significant research value for the industrial preparation of D-allulose.

The invention discloses a protein fiber dyeing method, a protein fiber and a fabric, according to the protein fiber dyeing method, reducing sugar DHA is reacted with indigo blue, and low-temperature green dyeing and functional modification of the protein fiber are synchronously realized by utilizing the dual functions of a reducing agent and a color developing agent of DHA. Specifically, ketose-dihydroxy acetone (DHA) is used for reducing indigo into a leuco body, meanwhile, the ketose-dihydroxy acetone (DHA) and amino in protein fibers are subjected to a Maillard reaction to generate yellowish-brown pigment, and a unique yellow-green system is formed by combining a blue system of traditional indigo, so that the dyeing chromatography is widened, meanwhile, the dyeing process is green and environmentally friendly, and the dyeing efficiency is improved. The dyed protein fiber is endowed with excellent anti-ultraviolet performance (UPFgt; 1800) and oxidation resistance (the free radical scavenging rate can reach 70%), and the strength of the dyed protein fiber is almost not lost after dyeing.

Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

The present invention discloses a method for analyzing the composition of monosaccharides in polysaccharides. Trifluoroacetic acid is added to the polysaccharide for hydrolysis to generate monosaccharides; the monosaccharides are dissolved in an organic solvent and then sodium hydroxide is added for alkalization to obtain alkalized monosaccharides; iodomethane is added to the alkalized monosaccharides for methylation to obtain methylated sugars, which are then analyzed by GC-MS. The entire polysaccharide processing process has mild conditions, simple operation, and is environmentally friendly. The analytical method proposed in the present invention can analyze the composition of ten monosaccharides in polysaccharides. It can be used not only for the analysis of aldoses, but also for the analysis of ketoses (fructose), with a clear chemical reaction equation; the analysis has high sensitivity and can complete qualitative and quantitative analysis of monosaccharides below 10μg.

The application discloses application of 1-chloro-4-hydrazinyl phthalazine as a reactive matrix in monosaccharide MALDI MSI in-situ analysis. The monosaccharide includes glucose, fructose, fucose and arabinose. The application develops 1-chloro-4-hydrazinyl phthalazine (CHP) as a reactive matrix, which is used for derivatization of monosaccharide in biological tissues and in-situ differentiation of monosaccharide isomers. Taking carrot as a model, monosaccharide on the carrot tissue is derivatized, and a MALDI-MSI experiment is carried out. It is worth noting that characteristic fragments generated by secondary mass spectrometry of the derivatization product can be used for further distinguishing aldoses and ketoses, thereby differentiating monosaccharide isomers and revealing different spatial distributions of monosaccharide isomers in organisms.

The present application relates to ketose crystals.

An L-rhamnose isomerase which can be obtained from a microorganism belonging to the genus Erwinia, has a subunit of a molecular mass of about 48 kDa as determined by SDS-PAGE, and has the substrate specificity of (A) and (B) described below. (A) has an isomerase activity which recognizes and reacts with CHO group at C1 and OH group at C2 of an aldose, converts CHO group at C1 into OH group, and converts OH group at C2 into CO group, or recognizes and reacts with OH group at C1 and CO group at C2 of a ketose, converts OH group at C1 into CHO group, and converts CO group at C2 into OH group. (B) has an activity of catalyzing isomerization between L-rhamnose and L-rhamnulose, between L-lyxose and L-xylulose, between L-mannose and L-fructose, between D-ribose and D-ribulose, between L-talose and L-tagatose, and between D-allose and D-psicose.

The application discloses application of a phenylpropionic acid compound in detection of monosaccharide composition of traditional Chinese medicine polysaccharide. The phenylpropionic acid compound has a structure as shown in formula (I). The application provides a new application of the phenylpropionic acid compound, and preliminary realization of quality control research on traditional Chinese medicine preknowing polysaccharide. Compared with a method for detecting monosaccharide composition of traditional Chinese medicine polysaccharide by using 1-phenyl-3-methyl-5-pyrazolone as a pre-column derivatization reagent, the application has the following advantages: 1. Derivatization of aldehyde sugar and ketose can be simultaneously realized; 2. The reaction temperature is more moderate, and the safety of the reaction is greatly improved; 3. The detection wavelength of HPLC-UV is improved from 245 nm to 274 nm, interference is reduced, and sensitivity is improved; and 4. The pre-column derivatization reagent is low in cost and easy to obtain.

The application discloses a surface treatment agent for a lithium ion battery positive electrode material and application thereof. The surface treatment agent for the lithium ion battery positive electrode material comprises monosaccharide or oligosaccharide. The monosaccharide comprises one or more of aldehyde sugar and ketose; the oligosaccharide comprises one or more of lactose, fructooligosaccharide, maltose, cellobiose, trehalose, raffinose, melezitose and gentiobiose. The surface treatment agent forms strong binding force with transition metals on the surface of the positive electrode material, weakens the solvation of ester solvents in electrolyte to the surface transition metals, prevents the surface transition metals from dissolving in the electrolyte and depositing to the negative electrode side. Meanwhile, the surface transition metals contained in the process weaken and inhibit the catalytic decomposition process of the electrolyte, thus the decomposition of the electrolyte on the surface of the high-voltage material is weakened and inhibited, the deposition of electrolyte decomposition products on the positive electrode side is reduced, and the cycle of the battery is more stable.

To provide integrated processes for catalytic conversion of carbohydrate to ethylene glycol and / or propylene glycol using a homogeneous, tungsten-containing retro-aldol catalyst.SOLUTION: A carbohydrate is subjected to retro-aldol conversion and hydrogenation. Ethylene glycol and propylene glycol are separated from the reaction product for purification, and at least a portion of the remaining fraction is subjected to ion exclusion chromatography to provide an eluant containing tungsten species and a subsequent eluant containing organic acids and a substantially reduced concentration of tungsten species. At least a portion of the eluant containing tungsten species can be recycled for reuse directly or with intervening unit operations to enhance the catalytic activity of the tungsten species. The organic-containing fraction can be subjected to unit operations to provide salable products or subjected to selective hydrogenolysis to lower glycols.SELECTED DRAWING: Figure 1

Integrated processes are disclosed for the catalytic conversion of carbohydrate to ethylene glycol and / or propylene glycol using a homogeneous, tungsten-containing retro-aldol catalyst. In these processes, the carbohydrate is subjected to retro-aldol conversion and hydrogenation to provide a reaction product containing ethylene glycol and / or propylene glycol, other reaction process including organic acids, itols and tungsten species. Ethylene glycol and propylene glycol are separated from the reaction product for purification, and at least a portion of the remaining fraction is subjected to ion exclusion chromatography to provide an eluant containing tungsten species and a subsequent eluant containing organic acids and a substantially reduced concentration of tungsten species. At least a portion of the eluant containing tungsten species can be recycled for reuse directly or with intervening unit operations to enhance the catalytic activity of the tungsten species. The organic-containing fraction can be subjected to one or more unit operations to provide salable products or subjected to selective hydrogenolysis to lower glycols.

The present application provides phosphoketolase mutants, related products thereof and applications thereof in production of acetyl phosphate, acetyl-CoA and / or 3-hydroxypropionic acid. The phosphoketolase mutants provided by the present application have high catalytic capacity for both six-carbon ketose and five-carbon ketose, and can catalyze the conversion of six-carbon ketose and five-carbon ketose into acetyl phosphate with high efficiency, and then efficiently generate subsequent products acetyl-CoA and 3-hydroxypropionic acid, thereby having great application potential in production of acetyl-CoA and products (such as 3-hydroxypropionic acid) taking acetyl-CoA as a precursor.

Integrated processes are disclosed for the catalytic conversion of carbohydrate to ethylene glycol and / or propylene glycol using a homogeneous, tungsten-containing retro-aldol catalyst. In these processes, the carbohydrate is subjected to retro-aldol conversion and hydrogenation to provide a reaction product containing ethylene glycol and / or propylene glycol, other reaction process including organic acids, itols and tungsten species. Ethylene glycol and propylene glycol are separated from the reaction product for purification, and at least a portion of the remaining fraction is subjected to ion exclusion chromatography to provide an eluant containing tungsten species and a subsequent eluant containing organic acids and a substantially reduced concentration of tungsten species. At least a portion of the eluant containing tungsten species can be recycled for reuse directly or with intervening unit operations to enhance the catalytic activity of the tungsten species. The organic-containing fraction can be subjected to one or more unit operations to provide salable products or subjected to selective hydrogenolysis to lower glycols.

The invention provides a method for synthesizing erythritol from formaldehyde and dihydroxy acetone, which comprises the following step: by taking the formaldehyde and the dihydroxy acetone as substrates, spontaneously reacting in a buffer solution containing glycine ions and / or a buffer solution containing hydrogen phosphate ions to generate the erythritol. The invention provides a new route for synthesizing the erythritol by using the one-carbon compound formaldehyde, formaldehyde and dihydroxy acetone are taken as substrates and spontaneously react in a buffer solution containing glycine ions and / or a buffer solution containing hydrogen phosphate ions to generate the erythritol, the reaction can be carried out at normal temperature and normal pressure, and the yield is high. And the method can be carried out in a wide pH range, and has a good application prospect.

Compositions and methods are provided for producing dihydrofurans by glycosyl hydrolases capable of dehydrating 2-keto-3-deoxy-gluconic acid (KDG) to K4. Compositions and methods are also provided for further processing K4 to produce HMFA (5-hydroxymethyl-2-furoic acid) and / or FDCA (2,5-furandicarboxylic acid).