105 results about "Xylulose" patented technology

The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and / or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.

A novel xylose isomerase nucleotide sequence obtained from a bovine rumen fluid metagenomic library and also provides the amino acid sequence encoded by the nucleotide sequence, and a vector and a transformant containing the nucleotide sequence. When the xylose isomerase is expressed, a host cell is endowed with the capability of converting xylose into xylulose, and the xylulose is further metabolized by the host cell. Therefore, the host cell can take the xylose as a carbon source for growth. The xylose isomerase from a new source is expressed with high activity in Saccharomyces cerevisiae and is a mesophilic enzyme with optimal temperature of 60° C.

The invention relates to a recombinant yeast, a construction method thereof and application thereof for producing tyrosol and a derivative. The recombinant yeast is acquired by introducing an exogenous fructose-6-phosphate phosphoketolase into an engineered yeast cell, and the engineered yeast cell is a yeast cell having a metabolic pathway for the synthesis of tyrosol via erythrose-4-phosphate and phosphoenolpyruvate. It is disclosed for the first time that in the process of expressing the fructose-6-phosphate phosphoketolase in the yeast, fructose-6-phosphate is catalyzed into erythrose-4- phosphate and acetyl phosphate while synthesizing 1,6-fructose diphosphate, xylulose-5-phosphate is catalyzed into glyceraldehydes-3-phosphate and acetyl phosphate, which alters the distribution of carbon metabolism in yeast, and enhances the synthesis of erythrose-4-phosphate, an important intermediate of tyrosol biosynthesis. The metabolic pathway of synthetic tyrosol is optimized, and the yieldof derivatives such as tyrosol and hydroxytyrosol is improved.

Genetic recombinant yeast expressing xylose reductase (XR), (wild-type or mutant) xylitol dehydrogenase (XDH), and xylulokinase (XK) and a method for highly efficiently producing ethanol from xylose using the yeast are provided. Pichia stipitis-derived XR and (wild-type or modified-type) XDH genes and Saccharomyces cerevisiae-derived XK gene were introduced via chromosomal integration. Thus, a genetic recombinant yeast having a high xylose fermentation rate, being capable of producing ethanol from xylose in high yields, and having high xylose fermentability in the presence of glucose, as well as a method using the recombinant yeast for highly efficiently producing ethanol from xylose or a saccharified solution from lignocellulose-based biomass are provided. Furthermore, a method for improving the xylose fermentability of the genetic recombinant yeast of the present invention via acclimatization treatment is also provided herein.

The invention relates to an eukaryotic cell expressing nucleotide sequences encoding the ara A, ara B and ara D enzymes whereby the expression of these nucleotide sequences confers on the cell the ability to use L-arabinose and / or convert L-arabinose into L-ribulose, and / or xylulose 5-phosphate and / or into a desired fermentation product such as ethanol. Optionally, the eukaryotic cell is also able to convert xylose into ethanol.

There is provided the use as reducing agents of alpha-hydroxycarbonyl compounds capable of forming cyclic dimers. There is also provided corresponding methods of reducing reducible compounds, particularly reduction-activated prodrugs . Examples of the alpha-hydroxycarbonyl compounds used are dihydroxyacetone, glycolaldehyde , glyceraldehyde, erythrose, xylulose, erythrulose or 3-hydroxy-2-butanone.

The invention discloses a corynebacterium glutamicum engineering strain for biosynthesis of rare sugar, and a building method and application thereof, and discloses a corynebacterium glutamicum recombination strain SY10. An experiment proves that rare ketose and deoxidized ketose can be synthesized by adopting a plurality of hydroxyaldehydes and glucoses as substrates by the strain in a fermentation manner, for example, D-erythrulose can be synthesized by adopting formaldehyde and glucose as substrates, L-xylulose can be synthesized by adopting glycolaldehyde and glucose as substrates, D-sorbose and D-psicose can be synthesized by adopting D-glyceraldehyde and glucose as substrates, L-fructose can be synthesized by adopting L-glyceraldehyde and glucose as substrates, and 3R, 4S, 5R, 6R-heptose and 3R, 4R, 5R, 6R-heptose can be synthesized by adopting D-erythrose and glucose as substrates. Therefore, the corynebacterium glutamicum recombination strain SY10 disclosed by the invention can be applied to the field of production of the rare ketose and the deoxidized ketose by whole-cell fermentation; the produced rare ketose and deoxidized ketose have broad application prospects in the industries such as a food, a medicine and the like.