28 results about "Xylulose" patented technology

The invention discloses recombinant Escherichia coli for producing xylitol by efficiently utilizing L-arabinose without adding glycerol and application, and belongs to the technical field of genetic engineering. Escherichia coli with an araBAD operon and an L-xylulokinase gene lyxK knocked out is used as a starting strain, a glucose specific transporter gene ptsG and a D-xylulokinase gene xylB in a genome are knocked out, and a global regulatory factor CRP is mutated; on the basis of an araA, dpe and lxr three-enzyme expression universal method reported at present, the gene arrangement sequence and copy number in three-enzyme tandem expression are optimized, a promoter and an RBS sequence are optimized and screened, and three-enzyme genes are integrated to a specific position of an escherichia coli chromosome in an optimized multi-copy form. According to the method, the inhibition effect of glucose on L-arabinose is achieved, the in-vivo multienzyme catalysis efficiency of existing synthesis of xylitol from arabinose is remarkably improved, and the yield of precursor arabitol and the yield of the product xylitol are greatly improved.

The invention relates to a method for characterizing intracellular NADPH (Nicotinamide Adenine Dinucleotide Phosphate) generation amount of yarrowia lipolytica through xylose consumption, and belongs to the technical field of biology. According to the method, the yarrowia lipolytica overexpressing xylose reductase, xylitol dehydrogenase and xylulokinase is inoculated into a culture medium taking xylose as a unique carbon source for culture, and a linear relation between the xylose consumption and the NADPH generation amount is established, so that the generation level of the NADPH can be represented by measuring the xylose consumption in the culture medium. The invention provides an effective method for characterizing the intracellular NADPH level of the yarrowia lipolytica, the intracellular NADPH level can be characterized only by detecting the consumption of xylose, and the detection method is simple and efficient.

The invention provides a strain for producing beta-carotene as well as a construction method and application of the strain, and belongs to the technical field of synthetic biology and genetic engineering. The construction method of the MYy107 strain disclosed by the invention comprises the following steps: step 1, integrating a mevalonate kinase gene ERG12, a phosphomevalonate kinase gene ERG8, a mevalonate diphosphate decarboxylase gene ERG19 and an isopentene diphosphate isomerase gene IDI1 on an RT08 bacterium, so as to obtain an RT10 strain; 2, a xylose reductase gene XYL1, a xylitol dehydrogenase gene XYL2, a xylose isomerase mutant XylA3 * and a xylulokinase gene Yl.Xk are integrated on the RT10 strain, and the MYy107 strain is obtained. According to the MYy107 strain constructed by the invention, the beta-carotene can be produced by using xylose and acetic acid at the same time, and the yield of the beta-carotene is remarkably improved and reaches 710.01 mg / L.

The invention belongs to the technical field of biological enzyme engineering, and particularly relates to a D-psicose 3-epimerase mutant derived from rumen clostridium and application of the D-psicose 3-epimerase mutant. The mutant disclosed by the invention is obtained through specific single-point mutation on the basis of a wild type amino acid sequence of D-psicose 3-epimerase, and specifically, the mutant is obtained through mutation of the following five sites: L159V, Q164E, N238T, E288G and C289I; wherein mutants containing all five single point mutations are named as XT mutants. The mutants significantly improve the thermal stability of the enzyme and the catalytic conversion rate under high substrate concentration, especially under the high substrate concentration of 300-700 g / L, the conversion rate of D-psicose of the XT mutant is improved by 2-3% compared with that of a wild type, and the thermal stability of the XT mutant is improved by 28 times compared with that of the wild type. The mutant is suitable for a high-temperature and high-substrate-concentration industrial conversion system, the production cost of D-psicose can be remarkably reduced, and the mutant has important industrial application value.

The invention relates to a recombinant pichia pastoris strain for producing coupled xylitol by NADPH (nicotinamide adenine dinucleotide phosphate) dependent growth and a construction method of the recombinant pichia pastoris strain, and belongs to the technical field of microbial fermentation. The recombinant pichia pastoris strain provided by the invention contains an NADPH dependent xylitol dehydrogenase gene, a sugar phosphatase gene, a D-arabinol-4-dehydrogenase gene, a fructose-1, 3, 4-triazole-1, 3, 4-triazole-1, 3-triazole-1, 3-triazole-1, 3-triazole-1, 3- The gene sequence is as follows: a 1, 6-diphosphatase gene, a phosphoketolase gene, a phosphate transacetylase gene, a glucose-6-phosphate dehydrogenase gene, a 6-phosphogluconolactonase gene, a 6-phosphogluconate dehydrogenase gene and a ribulose-5-phosphate epimerase gene; gene for expressing xylulokinase is knocked out or down-regulated: NADH dependent type xylitol dehydrogenase gene, ribose-5-phosphate isomerase gene, glutamate dehydrogenase gene, phosphofructokinase gene and phosphoglucose isomerase gene. The yield of xylitol produced by fermentation of the strain is far higher than that in the prior art.

The invention belongs to the technical field of synthetic biology, and particularly relates to recombinant escherichia coli for efficiently biosynthesizing lanosterol as well as a construction method and application of the recombinant escherichia coli. The method comprises the following steps: introducing exogenous squalene synthase (SQS), squalene epoxidase (SE) and oxidized squalene cyclase (OSC) genes into escherichia coli to construct a complete lanosterol synthesis route; the method comprises the following steps of: enhancing MEP pathway rate-limiting enzymes, namely 1-deoxy-D-xylulose-5-phosphate synthase (DXS), isopentenyl pyrophosphate isomerase (IDI) and farnesyl pyrophosphate synthase (FPS), so as to increase precursor supply, and further regulating and controlling the expression of key enzyme genes by adopting an arabinose promoter (PBAD). The lanosterol yield of the constructed engineering bacterium BW-2 reaches 8.02 mg / L and is increased by 40 times compared with that of an initial strain, and a brand new technical scheme is provided for green and efficient industrial production of lanosterol.

The invention discloses a method for synthesizing xylitol by catalyzing D-xylose through a multi-enzyme system and a special engineering bacterium thereof. The invention provides a method for synthesizing xylitol from D-xylose, which specifically comprises the following steps: taking D-xylose as a substrate, generating xylulose under the catalysis of xylose isomerase, and generating xylitol under the action of xylulose reductase. For the production of xylitol, huge economic benefits can be generated, and the method has great popularization and application values.

The application discloses high-yield gernien A engineering methanotrophs, a construction method and application thereof, and belongs to the technical field of metabolic engineering and synthetic biology. P mxaF expressing a gernien A synthase encoding gene gas overexpressing a 1-deoxy-xylose-5-phosphate synthase encoding gene in a gernien A synthesis pathway dxs1 and dxs2 a farnesyl pyrophosphate synthase encoding gene ispA a isopentenyl pyrophosphate isomerase encoding gene Bsidi simultaneously overexpressing 6-phosphogluconate dehydrogenase zwf1 and pfk1 and fba2 in an EMP pathway, to obtain gernien A engineering methanotrophs. The fermentation production of gernien A is carried out by using the engineering methanotrophs, and the yield of gernien A is obviously improved, so that the engineering methanotrophs have an industrial application prospect.

There is provided an aqueous personal care rinse-off formulation, the aqueous personal care rinse-off formulation comprising: a dermatologically acceptable aqueous vehicle; a dermatologically acceptable cleansing surfactant, wherein the dermatologically acceptable cleansing surfactant comprises an anionic surfactant; and an alkyl polysaccharide thickener; wherein the alkyl polysaccharide thickener comprises from 50% to 100% by weight, based on the weight of the alkyl polysaccharide thickener, of a linear alkyl polysaccharide of formula (A), R-O-(S) nH (A) wherein R is a linear C9-15 alkyl group; wherein n is an average value from 1 to 2.5; wherein S is a monosaccharide selected from the group consisting of ribulose, xylulose, ribose, arabinose, xylose, lyxose and mixtures thereof; and wherein 70% to 100% by weight, based on the weight of monosaccharide S in the linear alkyl polysaccharide of formula A, of the monosaccharide S is xylose; wherein the aqueous personal care rinse-off formulation comprises lt based on the weight of the aqueous personal care rinse-off formulation; 0.01% by weight of an alkyl sulfate surfactant and an alkyl ether sulfate surfactant.

This invention discloses an engineered methanogenic bacterium that produces high levels of squalene, its construction method, and its applications, belonging to the fields of metabolic engineering and synthetic biology. This is achieved by using a strong promoter in the host bacterium (methanogenic bacterium). P mxaF Expression of squalene synthase encoding gene sqs Overexpression of the gene encoding 1-deoxy-xylulose-5-phosphate synthase in the squalene synthesis pathway dxs1 and dxs2 Farnesyl pyrophosphate synthase encoding gene ispA Isopentenyl pyrophosphate isomerase encoding gene My friends Simultaneously overexpressing glucose-6-phosphate dehydrogenase zwf1 and the EMP pathway pfk1 and fba2 An engineered methanogenic bacterium producing squalene was obtained. Using this engineered methanogenic bacterium for squalene fermentation production can significantly increase squalene yield and has excellent prospects for industrial application.

An L-rhamnose isomerase which can be obtained from a microorganism belonging to the genus Erwinia, has a subunit of a molecular mass of about 48 kDa as determined by SDS-PAGE, and has the substrate specificity of (A) and (B) described below. (A) has an isomerase activity which recognizes and reacts with CHO group at C1 and OH group at C2 of an aldose, converts CHO group at C1 into OH group, and converts OH group at C2 into CO group, or recognizes and reacts with OH group at C1 and CO group at C2 of a ketose, converts OH group at C1 into CHO group, and converts CO group at C2 into OH group. (B) has an activity of catalyzing isomerization between L-rhamnose and L-rhamnulose, between L-lyxose and L-xylulose, between L-mannose and L-fructose, between D-ribose and D-ribulose, between L-talose and L-tagatose, and between D-allose and D-psicose.

There is provided an aqueous personal care rinse-off formulation, the aqueous personal care rinse-off formulation comprising: a dermatologically acceptable aqueous vehicle; a dermatologically acceptable cleansing surfactant, wherein the dermatologically acceptable cleansing surfactant comprises an anionic surfactant; and an alkyl polysaccharide thickener; wherein the alkyl polysaccharide thickener comprises from 50% to 100% by weight, based on the weight of the alkyl polysaccharide thickener, of a linear alkyl polysaccharide of formula A, R-O-(S) nH (A) wherein R is a linear C9-15 alkyl group; wherein n is an average value from 1 to 2.5, and wherein S is a monosaccharide selected from the group consisting of ribulose, xylulose, ribose, arabinose, xylose, lyxose and mixtures thereof; and wherein 70% to 100% by weight, based on the weight of monosaccharide S in the linear alkyl polysaccharide of formula A, of the monosaccharide S is xylose.

This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyloin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds. These condensation reactions serve as a platform for the synthesis of isoprenoid precursors when utilized in combination with a variety of metabolic pathways and enzymes for carbon rearrangement and the addition / removal of functional groups. Isoprenoid alcohols are key intermediary products for the production of isoprenoid precursors in these novel synthetic metabolic pathways. These precursors can be modified to various isoprenoid products through prenyl transferase, terpene synthase, or terpene cyclases. The production of prenylated aromatic compounds is achieved through prenyl transfer of the hydrocarbon units of isoprenoid precursors to polyketides.

The invention belongs to the technical field of biomedicine, and particularly relates to application of xylulose-5-phosphoric acid as an inflammatory bowel disease biomarker with intestinal fibrosis characteristics. It is found for the first time that xylulose-5-phosphoric acid is remarkably increased in a biological sample of a patient with the inflammatory bowel disease with the intestinal fibrosis characteristic, and the expression level of xylulose-5-phosphoric acid in the biological sample of the patient with the inflammatory bowel disease with the intestinal fibrosis characteristic is in a positive correlation relation with the occurrence degree of the intestinal fibrosis. The discovery provides an important therapeutic target for predicting or evaluating whether a subject suffers from the inflammatory bowel disease with the intestinal fibrosis characteristic by using the xylulose-5-phosphoric acid as the inflammatory bowel disease biomarker with the intestinal fibrosis characteristic.

The application provides use of L-xylulose as a diagnostic marker for children with Kawasaki disease of immunoglobulin G non-responsiveness, and application of L-xylulose as a diagnostic marker in preparation of a Kawasaki disease diagnosis product, the diagnostic marker being a marker in a sample of children with Kawasaki disease, and the Kawasaki disease diagnosis product including a product for predicting and / or evaluating treatment effect of Kawasaki disease. The application first proposes a diagnostic marker of children with Kawasaki disease of immunoglobulin G non-responsiveness of L-xylulose, and uses the diagnostic marker for predicting and / or evaluating treatment effect of Kawasaki disease, and has good stability and repeatability.

The present disclosure relates to D-xylulose 4-epimerase, mutants thereof, and uses thereof. Specifically, the present disclosure relates to a polypeptide having D-xylulose 4-epimerase activity, a method for preparing said polypeptide, and use of said polypeptide in the preparation of L-pentose using D-xylose or D-xylulose as a raw material. Compared with the traditional preparation method in the prior art, the new method for preparing L-pentose discovered in the present disclosure has simpler production process and reduces the cost of producing L-pentose.

Provided herein are recombinant microorganisms having two or more copies of a nucleic acid sequence encoding a xylose isomerase, wherein the nucleic acid encoding the xylose isomerase is an exogenous nucleic acid. Optionally, the recombinant microorganism comprises at least one nucleic acid sequence encoding a xylulokinase and / or at least one nucleic acid sequence encoding a xylose transporter. The provided recombinant microorganism is capable of growing on xylose as a carbon source.

The invention discloses a method for preparing L-arabinose, and belongs to the technical field of preparation of L-arabinose. The invention particularly relates to a method for preparing L-arabinose by utilizing in-vitro biotransformation, which comprises the following steps: converting 6-phosphogluconic acid into D-ribulose 5-phosphoric acid by adopting 6-phosphogluconic acid dehydrogenase; the method comprises the following steps: converting D-ribulose 5-phosphoric acid into D-xylulose 5-phosphoric acid by adopting D-ribulose 5-phosphoric acid 3-epimerase; the method comprises the following steps: converting D-xylulose 5-phosphoric acid into L-ribulose 5-phosphoric acid by adopting L-ribulose 5-phosphoric acid 4-epimerase; under the action of isomerase and dephosphorizing enzyme, the L-ribulose 5-phosphoric acid is converted into the L-arabinose. The method is a brand-new biological method for preparing the L-arabinose, the L-arabinose is obtained with higher yield and higher purity, the purification process is simple, the production cost of the L-arabinose can be reduced, and the preparation process is environment-friendly.

The invention provides a platycodon grandiflorum 1-deoxy-D-xylulose-5-phosphate reductoisomerase PgDXR1 gene, the nucleotide sequence of the platycodon grandiflorum 1-deoxy-D-xylulose-5-phosphate reductoisomerase PgDXR1 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of a corresponding code is as shown in SEQ ID NO. 2. The invention further provides a plant overexpression vector containing the PgDXR1 gene and a genetically engineered host cell containing the PgDXR1 gene. In addition, the invention also provides an application of the PgDXR1 gene in regulation and control of synthesis of plant triterpenoid saponin. The invention provides the platycodon grandiflorum PgDXR1 gene related to synthesis of the triterpenoid saponin of the platycodon grandiflorum for the first time, and an expression product of the gene can positively regulate synthesis of the triterpenoid saponin; the invention provides new evidence for perfecting the synthetic route of the plant triterpenoid saponin, has important theoretical and practical significance for improving the quality of traditional Chinese medicinal materials, and provides a new way for regulating and controlling the content of the triterpenoid saponin of platycodon grandiflorum by utilizing genetic engineering.

The invention discloses a high-yield alpha-farnesene aerobic methanophilic engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of metabolic engineering and synthetic biology. The method comprises the following steps: expressing an alpha-farnesene synthase gene AFS by using a strong promoter PmxaF in host bacteria (aerobic methanophilic bacteria), overexpressing 1-deoxy-xylulose-5-phosphate synthase genes dxs1 and dxs2, a farnesyl pyrophosphate synthase gene ispA and an isopentenyl pyrophosphate isomerase gene Bsidi in a synthetic route of alpha-farnesene, and overexpressing 6-phosphate glucose dehydrogenase zwf1, the aerobic methanophilic engineering bacteria for producing the alpha-farnesene are obtained. When the methanophilic engineering bacterium is used for fermentation production, the yield of alpha-farnesene is obviously improved, and the methanophilic engineering bacterium has industrial application prospects.

The invention belongs to the technical field of genetic engineering, and particularly relates to recombinant foal foal xylose as well as a construction method and application thereof. The recombinant foal foal capable of improving the bacterial cellulose yield and the xylose utilization amount is constructed by taking the foal foal foal as an original strain and overexpressing a xylose isomerase gene xylA, a xylulokinase gene xylB, a phosphopentose pathway key gene TKL and a structural gene BcsD. Under the fermentation conditions of pure xylose and xylose and glucose, the bacterial cellulose yields of the bacterial strain can reach 9.51 g / L and 8.1 g / L respectively and are increased by 553.23% and 44.75% respectively compared with original bacteria, and the highest xylose consumption of the bacterial strain under the fermentation conditions of pure xylose can reach 11.18 g / L. According to the method, the utilization rate of xylose is remarkably increased, the production efficiency of bacterial cellulose is improved, and a feasible solution is provided for replacing efficient conversion of traditional biomass resources with glucose as a carbon source.

The application discloses a synthesis method of xylulose-5-phosphate. The method takes a commercially available compound 1 as a starting material, and obtains the target product xylulose-5-phosphate through seven independent step-by-step reactions. The intermediates are compound 2 to compound 7 in turn. The synthesis method provided by the application is a step-by-step reaction, each reaction is an independent unit, the process parameters are accurately controllable and suitable for industrial operation errors, the side reactions are few, and the product selectivity is high. The method innovatively uses tetrabenzyl pyrophosphate as a phosphorylation reagent, replaces expensive reagents, and reduces the synthesis cost. The reaction condition of the method is mild, the operation is convenient, the crude compound 2 and the crude compound 4 can be directly used in the next step reaction, the post-treatment process is simple, and the route is simple and easy to be industrialized. The synthesis method of xylulose-5-phosphate has stable yield and accurate product structure, is suitable for large-scale industrialized preparation of xylulose-5-phosphate, and can effectively meet the market demand in the field of biological medicines.

The invention belongs to the technical field of microbial genetic engineering and industrial fermentation, and discloses an aspergillus niger engineering strain for producing L-malic acid by using xylose and a construction method and application of the aspergillus niger engineering strain. A three-gene recombination strain of an XR gene for coding xylose reductase, an XDH gene for coding xylitol dehydrogenase and an XK gene for coding xylulokinase through simultaneous overexpression is obtained through directional construction. Through synergistic overexpression of the XR gene, the XDH gene and the XK gene, the limitation that the starting strain LH-1 cannot utilize the xylose is successfully broken through, and effective utilization and directional conversion of the xylose are realized. The strain can be used for producing L-malic acid by using a low-cost xylose raw material, so that the production cost is remarkably reduced, a new technical path is provided for industrial production of organic acid, and the strain has a wide industrial application prospect.

The invention relates to a ribose-5-phosphate isomerase B mutant and application of the ribose-5-phosphate isomerase B mutant in preparation of rare sugar, and belongs to the technical field of biological engineering. The invention provides an ochrobactrum ribose-5-phosphate isomerase B mutant with relatively high activity and stability, a coding gene of the ochrobactrum ribose-5-phosphate isomerase B mutant, a recombinant expression vector containing a gene sequence, a recombinant expression transformant, a preparation method of a mutant enzyme preparation, and practical application of transforming biomass-derived D-xylose by using the mutant. According to the method, low-added-value biomass monosaccharide such as D-xylose can be converted into high-value rare sugar compounds such as D-xylulose and D-ribulose through a single-enzyme or multi-enzyme cascade reaction, the range of substrates and products is expanded, and the method has a wide application prospect.

The application provides use of L-xylulose as a diagnostic marker for children with Kawasaki disease of immunoglobulin G non-responsiveness, and application of L-xylulose as a diagnostic marker in preparation of a Kawasaki disease diagnosis product, the diagnostic marker being a marker in a sample of children with Kawasaki disease, and the Kawasaki disease diagnosis product including a product for predicting and / or evaluating treatment effect of Kawasaki disease. The application first proposes a diagnostic marker of children with Kawasaki disease of immunoglobulin G non-responsiveness of L-xylulose, and uses the diagnostic marker for predicting and / or evaluating treatment effect of Kawasaki disease, and has good stability and repeatability.

The present invention discloses xylose isomerases obtained based on an ancestral sequence construction method and their applications. These xylose isomerases are obtained based on the ancestral sequence construction method and have any of the amino acid sequences ID NO. 1 to SEQ ID NO. 4. Expression of these xylose isomerases alone or in combination can confer upon yeast cells the ability to convert xylose to xylulose, thereby conferring upon host cells the ability to convert xylose to other products. The present invention also relates to the application of these four xylose isomerases in yeast production of chemicals such as ethanol using xylose as a substrate. When expressed in yeast cells such as Saccharomyces cerevisiae, these xylose isomerases can confer upon a host cell that originally lacks the ability to convert xylose to xylulose this ability, conferring upon the host cell the ability to produce chemicals such as ethanol using xylose or lignocellulose hydrolysate, which are rich in xylose.

The invention discloses a beta-substituted phosphinamine mycin analogue as well as a preparation method and application thereof, and belongs to the field of plant protection. The molecular structure of the beta-substituted phosphinamine mycin analogue is formed by connecting two pharmacophores of phosphonic acid (ester) and hydroximic acid of phosphinamine mycin through a beta-substituent-containing propyl chain, the beta-substituted phosphinamine mycin analogue is obtained through beta-substituent structure optimization, and the structural general formula of the beta-substituted phosphinamine mycin analogue is as shown in formula (I). The compound acts on 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) in a 2-C-methyl-D-erythritol 4-phosphate path (MEP), is a herbicidal active compound with a new action mode, is high in herbicidal activity and safe to crops, and can be applied as a novel herbicide. Formula (I)

The invention belongs to the technical field of bioengineering, and relates to a recombinant engineering bacterium and application thereof in preparation of mannitol, and the recombinant engineering bacterium is a plasmid-free strain for producing a cofactor NAD < + > by using cheap xylose and nicotinamide. The following genes are knocked out from the strain: a gene ptsG for coding protein EIICBGlc in a glucose PTS system, mtlA for coding EIICA protein in D-mannitol transmembrane transport, a D-mannitol 1-phosphate dehydrogenase gene mtlD in a D-mannitol utilization path, purine de novo synthesis regulation protein purR, and srlAE in a sorbitol utilization gene cluster. The following genes are integrated in a genome of the strain: glucose transport assisting protein glf, glucokinase glcK, xylose utilization gene cluster xylose isomerase xlyA and xylulose phosphorylase xlyB, phosphoribose pyrophosphate synthetase prs, polyphosphate kinase ppk2, nicotinamide phosphoribose transferase nadV and nicotinamide mononucleotide adenylate transferase nadM. The strain is fermented by using glucose and xylose, and provides chassis bacteria for reaction needing cofactor circulation.