100 results about "Xylulose" patented technology

The present invention relates to host cells transformed with a nucleic acid sequence encoding a eukaryotic xylose isomerase obtainable from an anaerobic fungus. When expressed, the sequence encoding the xylose isomerase confers to the host cell the ability to convert xylose to xylulose which may be further metabolised by the host cell. Thus, the host cell is capable of growth on xylose as carbon source. The host cell preferably is a eukaryotic microorganism such as a yeast or a filamentous fungus. The invention further relates to processes for the production of fermentation products such as ethanol, in which a host cell of the invention uses xylose for growth and for the production of the fermentation product. The invention further relates to nucleic acid sequences encoding eukaryotic xylose isomerases and xylulose kinases as obtainable from anaerobic fungi.

The invention relates generally to the field of industrial microbiology and butanol production from sources of 5-carbon sugars such as lignocellulosic hydrolysates. More specifically, the invention relates to the use of an xylulose or xylulose-5-phosphate-producing enzyme and micro-aerobic or anaerobic conditions to increase butanol production from such sugars and recovery of said butanol through ins situ product recovery methods.

The invention discloses recombinant saccharomyces cerevisiae for producing dammarenediol and protopanoxadiol using xylose and a construction method. The construction method comprises the steps of replacing a promoter of a saccharomyces cerevisiae xylulokinase gene XKS1 with a promoter PFBA1 by virtue of a homologous recombination method, introducing xylose reductase XYL1 and a xylitol dehydrogenase XYL2 expression cassette, increasing the activities of transketolase TKL1 and transaldolase TAL1 so as to obtain recombinant bacteria 1, introducing a farnesyl-diphosphate farnesyltransferase gene ERG9, a squalene monooxygenase gene ERG1 and a dammarenediol synthase gene DS into the recombinant bacteria 1 so as to obtain recombinant bacteria 2, and introducing a nicotinamide adenine dinucleotide-hydroxymethylglutaryl coenzyme A reductase gene NADH-HMGr, farnesyl diphosphatesynthase ERG20 and a protopanoxadiol synthase-cytochrome P450 reductase fusion protein gene PPDS-ATR1 into the recombinant bacteria 2, so as to obtain recombinant bacteria 3. According to the recombinant saccharomyces cerevisiae, dammarenediol and protopanoxadiol can be artificially synthesized by virtue of xylose.

The invention discloses a corynebacterium glutamicum engineering strain for biosynthesis of rare sugar, and a building method and application thereof, and discloses a corynebacterium glutamicum recombination strain SY10. An experiment proves that rare ketose and deoxidized ketose can be synthesized by adopting a plurality of hydroxyaldehydes and glucoses as substrates by the strain in a fermentation manner, for example, D-erythrulose can be synthesized by adopting formaldehyde and glucose as substrates, L-xylulose can be synthesized by adopting glycolaldehyde and glucose as substrates, D-sorbose and D-psicose can be synthesized by adopting D-glyceraldehyde and glucose as substrates, L-fructose can be synthesized by adopting L-glyceraldehyde and glucose as substrates, and 3R, 4S, 5R, 6R-heptose and 3R, 4R, 5R, 6R-heptose can be synthesized by adopting D-erythrose and glucose as substrates. Therefore, the corynebacterium glutamicum recombination strain SY10 disclosed by the invention can be applied to the field of production of the rare ketose and the deoxidized ketose by whole-cell fermentation; the produced rare ketose and deoxidized ketose have broad application prospects in the industries such as a food, a medicine and the like.

The invention discloses a method for extraction of xylose isomerases by taking brown hot crack loving spore fungus as sources and the activity expression and application of the brown hot crack loving spore fungi xylose isomerases in Saccharomyces cerevisiae. The molecule weight of the brown hot crack loving spore fungi xylose isomerases is 43 kilodaltons; the most suitable reaction temperature of the xylose isomerases is 80 to 85 DEG C; and the most suitable reaction pH of the xylose isomerases is 7.0. The xylose isomerases can convert xyloses into xyluloses under normal biochemical reaction conditions; genes of the xylose isomerases can be recombined into the Saccharomyces cerevisiae in which the activity expression is realized, and the xyloses or other carbon sources such as lignocellulose hydrolysate and so on can be utilized for production of various fermentation products such as alcohols and so on. The method utilizes the genetic engineering means to expand substrates which are converted by the alcohols and has important theoretical significance and application value on full utilization of lignocellulose resources.

The present invention is provides methods and formulations for preventing or ameliorating progression to chronic heart failure subsequent to cardiac stress, including as a consequence of myocardial infarction (MI), coronary artery disease, hypertension, cardiomyopathy, myocarditis, valvular regurgitation, severe lung disease, and/or severe anemia of chronic disease, by administration of one or more rate-limiting precursors to the synthesis of ATP. In one embodiment the ATP precursor is a pentose selected from one or more of ribose, D-ribose, ribulose, xylitol, xylulose, and a 5-carbon precursor of ribose.

The invention discovers that a xylose isomerase gene of non-glutinous rice has the function of converting xylose into xylulose. According to the invention, a recombinant plasmid containing the gene is constructed and is introduced into saccharomyces cerevisiae to obtain new engineered saccharomyces cerevisiae. Experiments prove that the saccharomyces cerevisiae which does not have the function ofconverting xylose into xylulose obtains the conversion capacity after the xylose isomerase gene is expressed in a host cell.

The invention discloses a rhodobacter sphaeroides engineering strain for producing beta-carotene and a construction method thereof. The method includes first optimizing a codon of a lycopene cyclase gene crtYPa derived from pantoea agglomerans to obtain an opt crtYPa gene, then replacing an endogenous phytoene three-step dehydrogenase gene crtI3 of rhodobacter sphaeroides with the opt crtYPa genecontaining a promoter prrnB and a phytoene four-step dehydrogenase gene crtIPa gene derived from the pantoea agglomerans in a traceless mode, then knocking out an endogenous neurosporene hydroxylase gene crtC of the rhodobacter sphaeroides and a 6-phosphoglucose dehydrogenase gene zwf and finally knocking out an endogenous 1-deoxyxylulose-5-phosphate synthase gene dxs of the rhodobacter sphaeroides with integration expression at zwf position to obtain the rhodobacter sphaeroides engineering strain for producing the beta-carotene. The strain is cultured by fermentation, and the content of the beta-carotene can reach 30mg/g DCW.

The invention discloses a salvia 1-deoxidation xylulose-5-phosphat synthase gene1, protein which is encoded by the salvia 1-deoxidation xylulose-5-phosphat synthase gene1 and the use thereof, which fills a gap that the 1-deoxidation xylulose-5-phosphat synthase gene1 is separated and cloned from salvia which is precious traditional Chinese medicine in China. The 1-deoxidation xylulose-5-phosphat synthase gene1 which is provided by the invention has a nucleotide sequence or a homologous sequence which adds, replaces, inserts or losses one or a plurality of nucleotides or allele thereof and the nucleotide sequence which is derived from the 1-deoxidation xylulose-5-phosphat synthase gene1, which are displayed in the SED ID No.1. The protein which is encoded by the gene has an amino acid sequence or the homologous sequence which adds, replaces, inserts or losses one or a plurality of amino acids, which is displayed in the SEQ ID No.2. The 1-deoxidation xylulose-5-phosphat synthase gene1 which is provided by the invention has prominent effect of increasing the content of tanshinone in salvia through the technology of excessive expression of the gene and can be widely applied in improving the quality of salvia mi ltiorrhiza, which has very good application prospect.

The invention relates to a method for selecting a 1-deoxy-D-xylulose-5-phosphate synthase inhibitor from a plant extract. The inbibitional effect of the added plant extract on phosphotriose isomerization activity of DXS enzyme is assessed by detecting the conversion quantity of a substrate with pre-column derivatization-high performance liquid chromatography is evaluated. The method mainly comprises the following steps of: adding the plant extract in a phosphotriose isomerization reaction system catalyzed by the DXS enzyme; after reaction at a certain temperature, hydrolyzing the phosphotriose in the reaction system with phosphatase; and next, adding 2,4-dinitrophenylhydrazine to the reaction system for derivatization and then performing detection by using an ultraviolet detector and taking methanol-water or acetonitrile-water as eluent gradients with high performance liquid chromatography. In the selection process of the method, the substrate D-glyceraldehyde 3-phosphoric acid (D-GAP) or dihydroxyacetone phosphate(DHAP) contains no isotope labeling; and the selection process is simple, quick and high in feasibility without any expensive large instrument for detection.

The invention discloses a red sage root 1-deoxy-xylulose-5-phosphate synthase II gene and its encoded proteins and applications, which fills the blank of separating the 1-deoxy-xylulose-5-phosphate synthase II gene from the traditional Chinese herb red sage root. The 1-deoxy-xylulose-5-phosphate synthase II gene provided by the present invention has the nucleotide sequence shown by the SEQ ID No.1 or homology sequence with one or a plurality of added, replaced, inserted or deleted nucleotide and its allelomorphic gene as well as it derived nucleotide sequence; the encoded protein has amino acid sequence shown in SEQ ID No.2 or r homology sequence with one or a plurality of added, replaced, inserted or deleted nucleotide. The 1-deoxy-xylulose-5-phosphate synthase II gene plays a significant role for improving the tanshinone content in the red sage root by a gene excess expression technique.

This disclosure generally relates to the use of enzyme combinations or recombinant microbes comprising same to make isoprenoid precursors, isoprenoids and derivatives thereof including prenylated aromatic compounds. Novel metabolic pathways exploiting Claisen, aldol, and acyioin condensations are used instead of the natural mevalonate (MVA) pathway or 1-deoxy-d-xylulose 5-phosphate (DXP) pathways for generating isoprenoid precursors such as isopentenyl pyrophosphate (IPP), dimethylallyl pyrophosphate (DMAPP), and geranyl pyrophosphate (GPP). These pathways have the potential for better carbon and or energy efficiency than native pathways. Both decarboxylative and non-carboxylative condensations are utilized, enabling product synthesis from a number of different starting compounds. These condensation reactions serve as a platform for the synthesis of isoprenoid precursors when utilized in combination with a variety of metabolic pathways and enzymes for carbon rearrangement and the addition/removal of functional groups. Isoprenoid alcohols are key intermediary products for the production of isoprenoid precursors in these novel synthetic metabolic pathways.

The invention discloses a method for preparing D-allose by reducing ketose by a catalytic hydrogenation process, which comprises the following steps: 1) subjecting the compound of formula (II) to reduction reaction in the presence of a transitional metal catalyst and hydrogen to obtain a compound of a formula III, wherein R1 and R2 are dihydroxyl protective groups; and 2) removing the R1 and R2 protective groups from the compound of the formula III to obtain D-allose. The method has the advantages of high stereoselectivity, high cleanness, convenience for post treatment and the like and is a preparation method suitable for industrial production.

It is intended to provide a radioactive diagnostic imaging agent comprising a radioactive halogen-labeled compound as an active ingredient, in which the active ingredient is prevented from radiolysis and its stability is improved. This is achieved by adding a biologically-acceptable sugar or sugar alcohol to the radioactive diagnostic imaging agent in an amount effective to prevent radiolysis. The amount of the sugar or sugar alcohol to be added is preferably 10 (mmol/L)/GBq/mL or more, and more preferably 50 (mmol/L)/GBq/mL or more. The sugar is preferably selected from the group consisting of erythrose, threose, ribose, arabinose, xylose, lyxose, allose, altrose, glucose, mannose, gulose, idose, galactose, talose, erythrulose, ribulose, xylulose, psicose, fructose, sorbose, and tagatose. The sugar alcohol is preferably selected from the group consisting of erythritol, xylitol, sorbitol, and mannitol.

The invention provides a method for increasing content of tanshinone, relating to the field of biotechnologies. The method comprises the following steps: using a Salvia miltiorrhiza 1-deoxidized xylulose-5-phosphoric acid phosphate synthetase (SmDXS) gene and a Salvia miltiorrhiza germa crone phosphate synthetase (SmGGPPS) gene to construct a plant bivalent efficient expression vector of the SmDXS gene and SmGGPPS gene; carrying out genetic transformation on Salvia miltiorrhiza lamina to obtain Salvia miltiorrhiza hairy roots with the genetically modified SmDXS gene and SmGGPPS gene. The genetically modified Salvia miltiorrhiza hairy roots provided by the invention have remarkably increased tanshinone content, the total tanshinone content of a strain is 6.29 times that of the like. The invention provides a method for increasing the content of tanshinone in the Salvia miltiorrhiza hairy roots and provides a novel quality material for producing the tanshinone in important clinical demands.

The invention belongs to the field of gene engineering, and discloses a construction method of a synthetic path for generating glutamic acid by utilizing xylose in corynebacterium glutamicum. The construction method comprises the following steps of (1) expressing heterogenous pentose transport protein in the corynebacterium glutamicum; (2) expressing heterogenous xylose isomerase and xylulokinasein the corynebacterium glutamicum; (3) generating alpha-ketoglutarate from generated 5-xylulosephosphate through a phosphopentose pathway and a glycolysis pathway, and carrying out weakened expressionon alpha-ketoglutarate dehydrogenase, so that more alpha-ketoglutarate flows to the synthesis of glutamic acid; and (4) modifying glutamic acid secretory protein to ensure that the corynebacterium glutamicum does not respond to external biotin pressure any more, and continuously secreting the glutamic acid out of cells. According to the constructed synthetic path from the xylose to the glutamic acid, the corynebacterium glutamicum can utilize the xylose to produce the glutamic acid in a conventional synthetic culture medium, and the synthesis from the xylose to the glutamic acid can also be conducted in a high-biotin environment such as lignocellulose.