127 results about "Recombinant zymomonas" patented technology

The invention relates to the technical field of gene engineering, and discloses a recombinant yeast strain, and a construction method and application thereof. In the recombinant yeast strain, gal1, gal7, gal10 and ypl062w genes are knocked out, and the recombinant yeast strain comprises 9 gene segments which are integrated to the genome by yeast homologous recombination. The construction method comprises the following steps: constructing a four-knock-out yeast strain to provide an optimized host cell for producing beta-carotin, selecting lycopene cyclases crtY from different sources, and carrying out modular design to integrate functional genes crtE crtB and crtI of the specific-source synthetic lycopene, specific yeast endogenous genes and the like to the four-knock-out yeast strain genome, thereby obtaining a brand-new recombinant strain capable of producing beta-carotin at high yield.

The invention relates to the technical field of gene engineering, and discloses a recombinant yeast strain, and a construction method and an application thereof. The recombinant yeast strain is obtained through the following steps: knocking out gal1, gal7, gal10 and ypl062w genes, integrating three gene fragments a genome through yeast homologous recombination, further knocking out an rox1 gene, and further integrating two gene fragments to the yeast genome. The gene knockout yeast strain constructed in the invention provides optimized host cells for production of lycopene; and combined functional genes crtE, crtB and crtI having specific sources and used for synthesizing the lycopene and specific yeast endogenous genes are selected and are integrated to the gene knockout strain genome through modular designing, so the brand new recombinant strain for highly yielding lycopene is obtained.

The invention relates to the technical field of gene engineering, and discloses a recombinant yeast strain, and a building method and application thereof. The recombinant yeast strain includes two specific gene segments recombined and integrated onto a genome in a homologous way through yeast. By selecting the specific group for forming a promoter combination and matching phaffia rhodozyma sourced phytoene synthetase and lycopene cyclase dual-function enzyme gene CrtYB, GGPP synthase CrtE, phytoene desaturase CrtI and specific yeast endogenous gene and the like, the substances are integrated onto the yeast strain genome through modular design; the yp1062w gene is knocked away through homologous recombination; one strain of fire-new recombinant yeast without an inducing agent is obtained; the high yield of the beta-renieratene is ensured.

Disclosed are xylose-fermenting recombinant yeast strains comprising heterologous PsXYL1, Ps XYL2, and PsXYL3, as well as methods of fermenting xylose to obtain ethanol using the recombinant yeast strain.

The invention discloses a recombinant yeast strain (Saccharomyces cerevisiae)) capable of efficiently metabolizing xylose, which is named SyBE005. The recombinant yeast strain has been collected in Common Microorganism Center of China Committee for Culture Collection of Microorganisms, the collection number is CGMCC No.6634, and the recombinant yeast strain has the capacity of efficiently metabolizing xylose to produce ethanol. The recombinant yeast strain SyBE005 can effectively ferment xylose to generate ethanol. The ethanol yield reaches 64% of the theoretical yield, and the yield of the byproduct xylitol is lower than 12%; and thus, the recombinant yeast strain can efficiently convert xylose into ethanol, and is an excellent strain utilizing cellulose hydrolysate.

Disclosed are xylose-fermenting recombinant yeast strains expressing xylose reductase, xylitol dehydrogenase, and xylulokinase and having reduced expression of PHO13 or a PHO13 ortholog, as well as methods of fermenting xylose to obtain ethanol using the recombinant yeast strains.

The invention relates to the technical field of gene engineering, and discloses a recombinant yeast strain, a construction method thereof and the application thereof. In the recombinant yeast strain, gal1, gal7, gal10 and ypl062w genes are knocked out, and the recombinant yeast strain comprises 6 gene segments which are integrated to the genome through yeast homologous recombination. On the above basis, the recombinant yeast strain is further integrated onto one gene segment of a yeast genome, so that a better recombinant yeast is obtained. According to the technical scheme of the invention, the yeast strain is constructed based on gene knock-out, and an optimized host cell for producing lycopene is provided. Meanwhile, functional genes crtE crtB and crtI of a specific source combination and used for the synthesis of lycopene, specific yeast endogenous genes and specific exogenous genes are selected. The above selected genes are integrated to the gene knock-out genome of the yeast strain, and then a brand-new recombinant strain capable of producing high-yield lycopene is obtained.

Recombinant yeast strains that saccharify, ferment and grow on insoluble and crystalline forms of cellulose are disclosed herein. The yeast strains express tethered cellulases including cellobiohydrolase, endoglucanase and β-glucosidase. The recombinant organisms are particularly suited for consolidated bioprocessing.

The invention discloses applications of glycosyltransferase and related materials thereof in the construction of engineering bacteria for producing ginsenoside Rb1 and Rg1. A glycosyltransferase genePn3-32 which can catalyze ginsenoside Rd to generate ginsenoside Rb1 can be successfully identified through a synthetic biological method; and the gene can simultaneously catalyze ginsenoside F1 to generate ginsenoside Rg1 and construct recombinant yeast producing the ginsenoside Rb1 and the ginsenoside Rg1. Through experiments, the constructed recombinant yeast producing the ginsenoside Rb1 and the ginsenoside Rg1 can simultaneously generate the ginsenoside Rb1 and the ginsenoside Rg1. Pn1-31, Pn-3-29, Pn3-31 and Pn3-32 glycosyltransferase genes in medicinal plant radix notoginseng are firstly utilized to continuously catalyze protopanaxadiol and protopanaxatriol to synthetize the ginsenoside Rb1, the ginsenoside Rg1 and corresponding intermediates, so that novel cases can be provided formicrobial strains to produce natural products.

The invention discloses recombinant zymomonas mobilis capable of producing ethanol by using xylose and a fermentation method thereof. The recombinant zymomonas mobilis GX1 capable of producing the ethanol by using the xylose is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC 3438. The controlling elements of Escherichia coli rrnB and related genes of xylose metabolism are fused to build plasmids with artificially fused operons for the first time; and recombinant zymomonas mobilis strains capable of producing the ethanol by using the xylose are obtained through a method of electrotransformation and acclimatization. The recombinant zymomonas mobilis can efficiently express exogenous genes, can efficiently produce the ethanol by using glucose and the xylose to ferment together at the temperature of between 30 and 34 DEG C, and can produce the ethanol by using corncob hydrolysate containing the xylose and the glucose. The strains have important significance for producing clean energy by effectively using waste lignocellulose and solving the current energy crisis.

The invention relates to the technical field of gene engineering, and discloses a recombinant plasmid and a recombinant yeast strain and an establishing method and application thereof. The recombinant plasmid comprises a gene segment which is formed by sequentially splicing a terminator ADH1t, a beta-carotene hydroxylase gene crtZ, a promoter FBA1p or TEF1p, a promoter TDH3p, a beta-carotene ketolase gene crtW and a terminator TDH2t. By selecting the specific terminators, the specific promoters and the exogenous genes crtZ and crtW, the gene segment capable of producing astaxanthin is formed; thus, the recombinant plasmid is obtained and converted into the specific brewing yeast strain, the high yield of astaxanthin is ensured, and a more economical, more efficient and simpler mode is provided for heterologous microorganism production of astaxanthin.

The invention discloses recombinant yeast and a preparation method thereof. The method comprises the following steps of: (1) transferring an expression vector I into host yeast to obtain recombinant yeast transferred into the expression vector I, and recording the recombinant yeast as yeast A; and (2) transferring an expression vector II into the yeast A to obtain recombinant yeast transferred into the expression vector I and the expression vector II, and recording the recombinant yeast of the expression vector I and the expression vector II as yeast B. Experiments prove that the recombinant yeast prepared by the preparation method does not carry any resistance genes; the transferred expression vectors have multiple copy numbers between 5 and 50, and the copies are all integrated into a genome of the host yeast; and exogenous gene products expressed by the recombinant yeast are safe food-grade products. The preparation method has the advantages of simple operation, time saving, labor saving and low cost, and thus the recombinant yeast and the preparation method thereof are suitable for popularization and application.

The invention provides a method for producing ethyl alcohol through crop stalks by use of recombinant zymomonas mobilis. According to the method, the crop stalks are used as a base material, aspergillus niger and trichoderma are mixed and fermented to produce a crude enzyme solution containing cellulase, the crop stalks are processed through the crude enzyme solution to enable cellulose in the crop stalks to be decomposed to produce reducing sugar, and finally recombinant zymomonas mobilis is used for fermenting the enzymatically-decomposed reducing sugar to produce ethyl alcohol. In the method, fermentation products of aspergillus niger and trichoderma are mixed according to the ratio of 1: 1 to obtain the cellulase crude enzyme solution, so that the product feedback inhibition is effectively controlled, and the cellulase crude enzyme with higher activity and yield is obtained. As zymomonas mobilis is inoculated in the enzymatically-decomposed fermentation liquor containing reducing sugar, the ethyl alcohol production efficiency through the crop stalks by use of zymomonas mobilis is greatly improved, and defects that the use range of a zymomonas mobilis substrate is narrow, and the crop stalks cannot be effectively utilized are overcome. According to the method, the conversion rate of ethyl alcohol through the crop stalks is 5%, that is, 100 g of the crop stalks can produce 5 g of ethyl alcohol.

The invention relates to a recombinant yeast strain capable of producing conjugated linoleic acid and an application thereof. In the invention, codon optimization is carried out on linoleate isomerase in propionibacterium acnes (PAI) gene to obtain an optimized gene opai, recombinant expression plasmid and recombinant Yarrowia lipolytica bacterium which contain the opai gene are prepared, and therecombinant Yarrowia lipolytica bacterium which expresses the opai gene is utilized to produce conjugated linoleic acid.

The invention discloses an efficient manufacturing method and application of antimicrobial peptide PR-39 of pig small intestine in pichia pastoris. The efficient manufacturing method provided by the invention comprises the following steps of: synthesizing a DNA (Deoxyribonucleic Acid) for coding PR-39 according to the feeding preference of the pichia pastoris; connecting an expression vector pGAPZalphaA, constructing a pGAPZalphaA-PR-39 eukaryotic expression vector; transforming a recombinant expression vector into a host cell and constructing an expression engineering bacterium; fermenting and culturing recombinant saccharomycete and expressing. The method provided by the invention can be used for efficiently expressing the antimicrobial peptide PR-39 in the host cell of the pichia pastoris by utilizing a gene engineering technology, has the advantages of high expression efficiency, simple separation and purification, low production cost, easiness in amplification and good stability, is suitable for large-scale industrial production and has a wide application prospect in the fields of preparing antibacterial medicines, fodder additives or food preservatives.

The invention relates to the technical field of bio-energy and discloses a recombinant yeast strain and a microbial mixed bacteria electricity generation method. According to the recombinant yeast strain disclosed by the invention, genes PDC1, PDC5, PDC6, ADHI and ADH4 are knocked-out, and genes LDH, XR, XDH and XKS are inserted. Meanwhile, a yeast-Shewanella mixed bacteria system taking glucose and xylose as a carbon source is constructed, and by modifying brewer's yeasts, the glucose and xylose can be metabolized to produce lactic acid, and the carbon source and electron donors are providedfor shewanella in the MFC, so that available carbon source spectrums of the shewanella are increased, and the recombinant yeast strain has excellent performance on the electrochemical effect and laysa foundation for researching biomass by human beings and identifying new energy conversion manners.

The invention provides a hansenula polymorpha recombination strain and application thereof in the biosynthesis of gentiopicroside. A recombination saccharomycete used for producing gentiopicroside is obtained by a method for conversion of low energy ion implantation mediated gentiana macrophylla genome total DNA in the saccharomycete, and is named as hansenula polymorpha of which the preservation number is CGMCC.No.8998. The hansenula polymorpha recombination strain and the application realize the technical breakthrough in the aspect of using the recombination saccharomycete to be subjected to biological fermentation to produce gentiopicroside, solves the source shortage of gentiopicroside, protects the gentianaceae plant resources of our country, and provides a new way for sustainable development of the gentianaceae plant resources.

The invention discloses recomposed yeast for asymmetrically transforming and preparing (S)-4-chlorine-3-hydroxy-ethylbutyrate, which is Saccharomyces cerevisiae for reducing enzyme gene and glucose dehydrogenase gene by the introduction of carbonyl. The invention further discloses a composition method of the recomposed yeast. The method for preparing (S)-4-chlorine-3-hydroxy-ethylbutyrate by using the recomposed yeast is that: 4-chloracetyl-ethyl-acetate is used as a substrate, glucose is used as an auxiliary substrate, and the recomposed yeast is used for the transformation reaction so as to prepare (S)-4-chlorine-3-hydroxy-ethylbutyrate. The recomposed yeast, without adding any auxiliary enzyme, can effectively catalyze 4-chloracetyl-ethyl-acetate into (S)-4-chlorine-3-hydroxy-ethylbutyrate, with optical purity e.e value larger than 98 percent and the transformation ratio to the substrate larger than 95 percent, thus reducing the production cost. Compared with other strains without the need to add expensive auxiliary enzyme, the recomposed yeast has high transformation ratio to the substrate and high optical activity.

The present disclosure relates to recombinant yeast strains capable of maintaining their robustness at high temperature as well as recombinant proteins expressed therefrom. The present disclosure also provides methods for using the recombinant yeast strain for making a fermentation product. The present disclosure further process a process for making recombinant yeast strains capable of maintaining their robustness at high temperature.