31results about How to "Synthesis Yield Increased" patented technology

The invention relates to the technical field of gene engineering, and discloses a recombinant yeast strain, a construction method thereof and the application thereof. In the recombinant yeast strain, gal1, gal7, gal10 and ypl062w genes are knocked out, and the recombinant yeast strain comprises 6 gene segments which are integrated to the genome through yeast homologous recombination. On the above basis, the recombinant yeast strain is further integrated onto one gene segment of a yeast genome, so that a better recombinant yeast is obtained. According to the technical scheme of the invention, the yeast strain is constructed based on gene knock-out, and an optimized host cell for producing lycopene is provided. Meanwhile, functional genes crtE crtB and crtI of a specific source combination and used for the synthesis of lycopene, specific yeast endogenous genes and specific exogenous genes are selected. The above selected genes are integrated to the gene knock-out genome of the yeast strain, and then a brand-new recombinant strain capable of producing high-yield lycopene is obtained.

The invention relates to a method for synthesizing two different grades of A-type zeolites by utilizing coal ashes. The method is characterized by using the coal ashes as raw material, carrying out pretreatment on the coal ashes, and then, fusing the coal ashes and sodium carbonate under a high temperature; mixing the coal ashes obtained after alkali fusion with water fully to dissolve out sodium silicate, silicon-aluminum ratio in the ingredients of the desiliconized coal ashes being ideal ratio for synthesizing the A-type zeolites; then, mixing and stirring the desiliconized coal ashes and a sodium hydroxide solution to obtain a silica-alumina gel; carrying out hydrothermal crystallization on the silica-alumina gel, carrying out washing till the pH value is neutral after the crystallization, and carrying out drying to obtain a type-I A-type zeolite; adding appropriate amount of sodium aluminate into desiliconized filtrate, wherein the desiliconized filtrate is a solution comprising the sodium silicate; and carrying out hydrothermal crystallization, washing and drying to obtain a type-II A-type zeolite. The method can enable effective constituents of aluminosilicate in the coal ashes to be used completely, and is an effective resource utilization mode.

The invention discloses an 18F labeled quinazoline class EGFR (Epidermal Growth Factor Receptor) positron tracer agent and a preparation method and application thereof and belongs to the field of chemical synthesis. The 18F labeled quinazoline class EGFR positron tracer agent has a chemical structure as shown in a formula II. The synthesized 18F labeled micro-molecular EGFR class positron tracer agent has the clinic values of diagnosis and therapeutic effect monitoring and particularly plays an important role in promoting personalized treatment. Besides, the invention further provides a one-step method, namely a method for fully-automatically synthesizing the 18F labeled quinazoline class EGFR positron tracer agent. The method has the characteristics of multiple purposes, high yield, high efficiency, short synthesis time, low cost and the like and can meet the requirements of large-scale production. The formula II is shown below.

The invention discloses an 18F-labeled quinazoline type EGFR (epidermal growth factor receptor) positron tracer, as well as a preparation method and an application thereof and belongs to the field of chemical synthesis. The 18F-labeled quinazoline type EGFR positron tracer disclosed by the invention has a chemical structure as shown in a formula II. The synthesized 18F-labeled small molecular EGFR type positron tracer has clinical value of diagnosis and monitoring of treatment curative effect and particularly plays an important role in promoting personalized treatment. In addition, the invention further provides the method for full-automatic synthesis of the 18F-labeled quinazoline type EGFR positron tracer by one step, the method has the characteristics of multiple purposes, large yield, high efficiency, short synthesis time, low cost and the like, and the requirements of large-scale production can be further met.

The invention belongs to the technical fields of genetic breeding and genetic engineering of edible fungi, and specifically relates to grifola frondosa glucan synthase as well as an encoding gene andapplication thereof. The encoding gene of glucan synthesis key enzyme-glucan synthase is obtained for the first time from clones of grifola frondosa mycelium genome by the invention; key roles of thegene in grifola frondosa mycelium growth and glucan synthesis are demonstrated by gene silencing; and the glucan synthase is over-expressed in grifola frondosa by adopting an genetic engineering technology so as to have thallus growth of recombinant strains and synthesis yield of glucan significantly increased. The grifola frondosa glucan synthase as well as the encoding gene and the application thereof are helpful for understanding synthesis mechanism of edible and medicinal fungal polysaccharides at molecular level, developing metabolic engineering study on edible and medicinal fungal polysaccharide synthesis, and providing important technical support and reference for producing glucan with stable quality by performing high-efficiency fermentation.

The invention discloses an 18F-labelled quinazolines irreversible EGFR (epidermal growth factor receptor) positive electron tracer agent, as well as a preparation method and application thereof. The 18F-labelled quinazolines irreversible EGFR positive electron tracer agent has a chemical structure represented by the formula II. The synthesized 18F-labelled small-molecular irreversible EGFR positive electron tracer agent has a clinical value for diagnosing and monitoring a treating effect and particularly plays an important role in pushing individual-based treatment. Furthermore, the invention further provides a one-step method for synthesizing the 18F-labelled small-molecular irreversible EGFR positive electron tracer agent. The method has the characteristics of multiple purposes, high yield, high efficiency, short synthesis time, low cost and the like and can meet a requirement on large-scale production.

The invention relates to a high-yield mesoporous silica nano-particle and a folic acid targeting modification method thereof. A preparation method for the high-yield mesoporous silica nano-particle comprises the following steps: 1) synthesis of a silica nano-particle: preparing the silica nano-particle with a diameter of 300 to 500 nm from ethyl orthosilicate by using a sol-gel method; and 2) preparation of a mesoporous silica nanometer carrier: removing a template of cetyl trimethyl ammonium bromide (CTAB) by using sodium chloride so as to prepare the mesoporous silica nanometer carrier with a pore size of 0.5 to 2 nm. Meanwhile, folic acid targeting modification of the mesoporous silica nano-particle is carried out. The invention has the beneficial effects that synthesis process is simple, nontoxic and fast; the prepared mesoporous silica nano-particle has pore size of 0.5 to 2 nm and synthesis output is as high as 500 to 1000 mg, substantially increased compared with the normal output of 10 to 100 mg in the prior art; and after folic acid targeting modification of the mesoporous silica nano-particle, the carrier has improved efficiency in entering tumor cells.

The invention discloses a method for constructing and optimizing an engineering strain for producing ectoine, and belongs to the technical field of biological engineering. The invention provides an Escherichia coli engineering bacterium ECT-LA capable of producing ectoine under a low-salt condition. The bacterium is based on escherichia coli E.coli BL21 (DE3) and comprises an ectoine synthetic gene cluster ectABC controlled by a T7 promoter and exogenous genes lysCC932T and asd; a preparation method of ectoine is optimized futher. The recombinant escherichia coli constructed by the invention takes glucose as a substrate, and the yield of ectoine can reach 60g/L after fed-batch fermentation is performed for 56h.

The invention discloses a method for full-automatically synthesizing a <18>F-marked pyrimidine acrylamide based EGFR positive-electron tracer agent through a one-step method, and belongs to the fieldof chemical synthesis. The method sequentially comprises the following steps that (1) <18>F ions from an accelerator and water are conveyed to a receiving bottle and guided into an anion exchange column; (2) <18>F ions in the anion exchange column are eluted with a mixed solution formed by strong alkali and weak acid salt, acetonitrile and ionic liquid, and then are conveyed to a reaction bottle;(3) precursor is dissolved in a DMSO or acetonitrile or DMF solution, a buffer solution is added into the former solution to enable pH of the former solution to be 2, and the obtained solution is added into the reaction bottle and heated under protection of inert gas; (4) the temperature is lowered, and coarse products in the reaction bottle are purified through a solid-phase extraction C18 pillaret; and (5) target products of a solution obtained in the step (4) are separated and collected, so that the positive-electron tracer agent is obtained. The method has the characteristics of multi-purpose, large yield, high efficiency, short synthesis time, low cost and the like, and can meet the requirement for large-scale production.

The invention relates to an ion exchange polymer microsphere and a synthesis method thereof. The microsphere has a micron size, is internally composed of a hydrophobic polymer, and has a solid and compact structure; and the outer part is composed of a charged hydrophilic polymer and has a nano-porous structure. By changing the types of the hydrophobic polymer and the charged hydrophilic polymer, the chemical components and the surface appearance of the microspheres can be regulated and controlled. The microspheres have uniform size and morphology, the preparation method is simple, large-scale production is easy, and the microspheres have wide industrial application value. The ion exchange polymer microsphere with the micro-nano hierarchical heterostructure has great value in adsorption and separation of various molecules, ions and particles, and is expected to be applied to the fields of medicine purification, food detection, environmental governance and the like.

The invention discloses a method for preparation and purification of four stereoisomers of a benzopyrene-DNA adduct. An in-vitro synthesis mode is adopted to synthesize the four stereoisomers of the benzopyrene-DNA adduct which is benzopyrene-7,8-dihydrodiol-9,10-epoxide-deoxyguanosine adduct (anti-BPDE-N<2>-dG), and through optimization of the in-vitro reaction conditions, the yield of the synthesized four stereoisomers of anti-BPDE-N<2>-dG is improved by more than 2 times when the method is compared with an existing synthesis method. A pi-hole effect is applied to chromatographic separationand purification of the stereoisomers for the first time, and the four stereoisomers can be separated completely in a short time through optimization of the chromatographic conditions, so that the chromatographic purification time is shortened greatly, and the purification efficiency is improved.

The invention provides a nucleic acid synthesis-oriented micro-reactor array chip and a preparation method thereof, the micro-reactor array chip with a high surface area is constructed by ink-jet printing of a nanoparticle droplet array on the surface of a synthetic chip and combination of a three-phase contact line regulation and control method and a high-temperature sintering technology at the same time, and the nucleic acid synthesis-oriented micro-reactor array chip is prepared. High-flux and high-load synthesis is realized, the overall synthesis yield of the synthetic chip is improved, and the method can be applied to the field of organic synthesis.

The invention discloses a method for increasing an exogenous synthesis yield of water-soluble phycocyanin, which belongs to the technical field of biological genetic engineering and microbial fermentation. According to the method, a TB culture medium is used as a contrast; an expression vector system is induced according to escherichia coli; the phycocyanin is synthesized by using the phycocyaninsynthase; the specific culture medium components and the culture temperature are selected, so that the synthesis yield of the water-soluble algae is greatly increased, the water-soluble algae producedby the method has the characteristic that the yield is 2.2 times higher than that of the water-soluble algae produced by control culture, and more efficient culture conditions are provided for the production of the water-soluble algae.

The invention relates to an ammonia-containing air purification method, which comprises the following steps that (1) the flow of ammonia-containing air is guided to a tower bottom inlet of a sprayingabsorption tower through a draft fan; (2) the ammonia-containing air rises through the tower bottom inlet; at the moment, the tower top sprays soft water, so that the ammonia-containing air and soft water generate reverse flow contact; (3) in the reverse flow contact process, ammonia gas in the ammonia-containing air is completely dissolved into water and descends to the tower bottom along with the liquid; obtained purified air continuously rises to the tower top to be emitted into the air; (4) soft water after the ammonia gas absorption at the tower bottom is conveyed to the tower top again through a circulation pump; (5) the ammonia-containing air to be purified is supplemented again for contact absorption; the absorption process is repeated. Inorganized ammonia gas in the ammonia-containing air is collected to form dilute ammonia water waste liquid; the ammonia-containing air is purified; in addition, the dilute ammonia water waste liquid is used as supplementing water of a carbonization system; the resources are reasonably utilized.

The invention discloses a synthetic process of alkene monomers. The synthetic process of alkene monomers comprises the following steps: dissolving raw materials in a solvent, well mixing, reacting to obtain a reaction product mixture, performing reduced-pressure distillation and washing, performing separation and drying to obtain the product. The invention smoothly synthesizes the product, has a high synthetic yield, and reduced production cost; the synthesized alkene monomers can be used to synthesize new polymer scale inhibitors with excellent anti-scaling performance for barium and strontium scale by way of homopolymerization or copolymerization.