The present application relates to a kind of efficient regeneration method with the
larch of
north China as explant, the method comprises the following steps:
sowing, disinfection, induction culture, elongation culture, rooting culture,
seedling and
transplanting.According to the technical scheme provided by the present application, the medium with specific formula is used, so that the
larch of
north China shows significant advantages in the process of propagation: high differentiation efficiency, large proliferation coefficient, good rooting rate.In addition, the method also has the characteristics of short
plant growth cycle, strong adaptability after
transplanting and the like.The
seedling after cultivation presents the characteristics of robust and straight, good growth and uniformity.The technical scheme of the present application significantly reduces the
seedling culture period, effectively reduces the production cost.By establishing the adventitious
bud induction system with the
larch of
north China as starting material, and successfully obtaining regenerated
plant, a new technical approach is provided for the rapid propagation of the larch of north China.Meanwhile, the present application also lays the foundation for the future establishment of efficient larch of north China transgenic
system.