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Extract method for lectin of leguminous plants

A plant lectin and extraction method technology, which is applied in the field of extraction of useful plant components, can solve the problems of chemical reagent components leftover and the like, and achieve the effects of overcoming chemical reagent components leftover and overcoming long extraction time.

Active Publication Date: 2009-08-19
YUNNAN KANGZHOU BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the prior art are: the former has more chemical reagent components left over, and the latter requires long-term soaking, extraction and low-temperature freezing and centrifugal separation at a low temperature of -10°C to +10°C, and the extraction time is longer than 48 hours

Method used

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  • Extract method for lectin of leguminous plants
  • Extract method for lectin of leguminous plants
  • Extract method for lectin of leguminous plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Preparation of kidney bean lectin

[0021] First take the dried kidney bean kernels, wash them with tap water for 3-5 minutes, remove the sundries, then dry them at low temperature or hang them in the sun. Mechanically pulverize and pass through a 60-mesh sieve to become soybean powder. Weigh 50g of soybean powder and put it into a polyethylene bag, add 250ml of 0.9% normal saline, and seal it with a sealing machine. Put it in the microwave and set the power to 800W. Divided into high, medium and low treatment intensity groups and a control group. In the high-intensity treatment group, the first microwave heating time was 60 seconds, and then placed in a 50-60°C water bath for 20 minutes, stirred 5 times during the period, and then the second microwave treatment was performed for 30 seconds, and then placed in a 100w ultrasonic cleaner with a capacity of 6L. Add 3 bags of treated samples in 4L water, treat for 10 minutes, then perform the third microwave t...

Embodiment 2

[0027] Example 2: Preparation of kidney bean lectin

[0028] In this embodiment, the processing capacity and method are the same as those in Embodiment 2. Only change the temperature setting to 30-40°C. It has been determined that in the temperature range of 30-40 ° C, the PHA extraction results of three microwave and ultrasonic combinations of high, medium and low are shown in Table 2.

[0029] Table 3 The extraction rate of kidney bean PHA by different microwave and ultrasonic combinations at 30-40℃

[0030]

Embodiment 3

[0031] Example 3: Preparation of lentil lectin

[0032] In this embodiment, two temperature treatments are adopted, namely, 40-60° C. for 2 hours and 4-10° C. refrigerator for 12-14 hours. Microwave and ultrasonic treatment were not applied.

[0033] The pretreatment mode of lentil is the same as embodiment 1. The crushed bean flour is passed through a 60-mesh sieve. Add the same physiological saline or phosphate buffer as in Example 1 at a ratio of 1:5. Put them into 40-60°C heat preservation container and 4-10°C refrigerator respectively. Stir once every 15-30min. The extraction time of the 40-60°C heat preservation extraction group is 2 hours, and the 4-10°C refrigerator treatment is 12-14 hours. The processing after extraction is the same as in Example 1. The results are shown in Table 4.

[0034] Table 4 Extraction rate of lentil lectin at 40-60℃ and 4-10℃

[0035]

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Abstract

A method for extracting bean phytohemagglutinin belongs to the extraction technical field of useful constituents of plants. The method is a water extraction method in which the extraction is performed at 40-60 DEG C based on the following steps: crushing bean seeds to 40-100 meshes, adding soak solution to extract bean husk at the temperature of 40-60 DEG C for 60-180min and then fish the bean husk, performing rough separation to remove bean dregs, centrifugally separating to obtain supernatant with the bean phytohemagglutinin, further performing separation and purification on the supernatant, condensing or drying the supernatant to obtain the phytohemagglutinin. Ultrasonic wave or microwave can be exerted in the whole or part of the extraction process, or the ultrasonic wave and the microwave are alternately exerted to perform auxiliary extraction. A traditional low-temperature soaking and low-temperature treatment method is completely discarded in the method, and practice proves that the method is a simple and efficient method for extracting the phytohemagglutinin in short time. Related detection proves that the obtained phytohemagglutinin can meet conventional experimental needs and medicinal needs. The method helps overcome the disadvantage of more chemical reagent composition residue and long extraction time of the products obtained by the prior art.

Description

technical field [0001] The invention belongs to the technical field of extracting useful components of plants, and in particular relates to the extraction of bean plant lectins. Background technique [0002] Lectin PHA is a non-immunogenic protein or glycoprotein, which widely exists in animals, plants and microorganisms (such as bacteria and viruses). The lectins of legumes are composed of 2 or 4 monomers, each monomer has a molecular weight of 25-30KD and has a binding sugar site. Among the thousands of discovered lectins, there are more than 600 lectins belonging to leguminous plants. Lectin content also varies widely among legumes. For example, the protein content of kidney beans is 17-23%, and the protein contains 2.4-5% PHA, which is one of the highest lectin-containing species. [0003] Lectins not only have the function of agglutinating blood cells, lymphocytes, sperm cells, etc., but also participate in some important physiological and pharmacological processes i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/42
Inventor 王敏康
Owner YUNNAN KANGZHOU BIOLOGICAL SCI & TECH
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