262 results about "Low temperature treatment" patented technology

The invention discloses a pennisetum purpureum gene PpbHLH148L and application thereof in improving the cold resistance of plants, and relates to the technical field of gene engineering. The nucleotide sequence of the gene is as shown in SEQ ID NO.5. After the gene is subjected to subcellular localization and expression condition analysis after cold stress, the gene is related to low-temperature tolerance. An overexpression vector of the gene is constructed, Agrobacterium tumefaciens is transferred, then Arabidopsis thaliana infection is carried out, cold tolerance determination is carried out on overexpressed Arabidopsis thaliana, phenotypic difference and physiological index difference between an overexpressed strain after cold treatment and a wild type are observed, and it is found that the gene can significantly improve the low-temperature stress tolerance of Arabidopsis thaliana. And in the low-temperature treatment of the pennisetum alopecuroides transgenic callus, the transgenic callus also shows higher tolerance than the wild callus at low temperature. The pennisetum purpureum gene PpbHLH148L can be used for low-temperature tolerance modification of plants, and has an application prospect in creation of low-temperature-resistant plant materials.

The invention relates to the technical field of totally-closed artificial light plant factory planting, in particular to a totally-closed artificial light plant factory tomato seedling raising method, which is characterized in that each stage and each link in the whole cycle process of tomato growth are accurately controlled, and the tomato seedling raising efficiency is improved. Comprising the steps of nutrient solution management, irrigation frequency management and seedling raising environment temperature control in the stages of seed treatment, substrate preparation, sowing and germination acceleration, seedling raising and the like. The selection of a planting light source in the seedling raising stage is obtained through creative labor on the basis of a large amount of experimental data; the formula of the nutrient solution is also obtained on the basis of tomato characteristics and a large amount of experimental data in combination with creative labor; and the technical prejudice of conventional means is overcome, and the temperature condition of low-temperature treatment is adopted. All the technical means are organically combined and mutually influenced and promoted, and the technical problem of excessive growth of tomatoes in an artificial light environment can be effectively solved.

According to the preparation method of the low-temperature humified coal gangue-agriculture and forestry solid waste porous slow-release humic acid water-soluble fertilizer, the coal gangue is subjected to magnetic separation and flotation to remove impurities, then the composite chlorinating agent is used for low-temperature chlorination, heavy metal is effectively removed, and meanwhile energy consumption and environmental risks are reduced. Then, under a controlled low-temperature condition, through microwave assistance and specific atmosphere adjustment, a humification reaction is triggered, meanwhile, formation of a porous structure of the material is triggered, and the water solubility and the slow release effect of the fertilizer are enhanced. According to the method, the resource utilization rate of the coal gangue is increased, the high-efficiency soil conditioner is effectively generated through an innovative low-temperature treatment process, and good environmental protection benefits and economic values are achieved. Through the structural design of the obtained fertilizer, the fertilizer can respond to environmental factors, the nutrient release rate is automatically adjusted, and plant absorption and soil health are optimized.

The present invention belongs to the technical field of agricultural planting, and in particular to a cold resistance test method for black wolfberry branches and a centrifuge thereof. The present invention provides a cold resistance test method for black wolfberry branches, comprising: S1: sampling, taking branches with a diameter of about 0.5-0.6 cm and uniform thickness, pruning and sealing; S2: pre-treating the sample, cleaning the stains outside the sample and refrigerating for standby use; S3: low-temperature treatment of the sample, placing the sample in a low-temperature program-controlled container, processing the temperature gradient setting, and maintaining the low temperature for a certain period of time, and then heating the sample; S4: obtaining a supernatant, adding an extract to the sample quantitatively, grinding it into a homogenate, placing the homogenate in a centrifuge for centrifugal separation, and taking a supernatant; S5: determining the MDA content of the supernatant.

The invention provides the cassava low-temperature-resistant MeSR45-2 gene cloned from cassava for the first time, the expression of the MeSR45-2 gene in cassava is inhibited under low-temperature stress, and research shows that the gene can significantly improve the low-temperature resistance of cassava, reduce the malondialdehyde content, proline cumulant and the like in cassava, reduce leaf damage under low-temperature treatment and improve the yield of cassava. Terminal buds under low-temperature treatment are not affected and can normally grow, and expression of various protein genes is regulated and controlled. The invention provides a new candidate gene for research on improvement of plant low temperature resistance and the like, and also provides a new gene resource for cultivation of a new variety of low temperature resistant cassava and construction of a new variety of transgenic cassava.

The invention relates to the technical field of cold resistance evaluation, in particular to a cold resistance detection device and a Chinese pennisetum cold resistance evaluation method based on spectral analysis. The device comprises a containing pipe, a reflux condensing pipe, an extension pipe, a liquid supplementing pipe and a control core. And when the balance weight plugging piece is jacked up by the airflow, the valve is jacked open by the core body, so that the solvent is supplemented. The method comprises the following steps: carrying out low-temperature treatment on a pennisetum alopecuroides detection sample, and detecting the low-temperature exudation concentration of potassium ions in a preset amount of solvent by atomic absorption spectrometry; after the heat treatment is finished, supplementing the solvent in the accommodating tube to a preset amount, and detecting the high-temperature exudation concentration of the potassium ions by atomic absorption spectrometry; and determining the low-temperature seepage rate of the potassium ions according to the low-temperature seepage concentration and the high-temperature seepage concentration, and evaluating the cold resistance of the pennisetum alopecuroides detection sample according to the low-temperature seepage rate. The method simultaneously realizes small dosage, high efficiency and low risk in the detection process, and ensures high efficiency and smooth proceeding of the detection work.

The invention discloses a paper composite filter material for reducing hydrocyanic acid and crotonaldehyde and a preparation method and application thereof, and the preparation method comprises the following steps: adding a gelator into a solvent to form a gelator solution; transferring the gelator solution into a liquid storage tank of an intaglio printing device, and printing and coating the gelator solution in the liquid storage tank onto a paper base material by using an anilox roller; the coated paper base material is conveyed to a low-temperature tunnel, so that nanofibers of a three-dimensional network structure are formed inside and on the surface of the paper base material in a self-assembly mode; and transferring the paper base material subjected to low-temperature treatment to a drying cylinder, and drying to obtain the paper composite filter material. According to the prepared paper composite filter material, the specific surface area in contact with smoke can be increased, the paper composite filter material can make full contact with the smoke in the process that the smoke passes through a filter stick, the interception capacity of the paper filter stick on hydrocyanic acid and crotonaldehyde in mainstream smoke of cigarettes is improved through amino and carboxyl groups on the surfaces of the nanofibers, and the sensory quality of the cigarettes with the paper filter stick is improved.

The present application belongs to the technical field of regeneration of oil-containing activated carbon, specifically a method for regenerating oil-containing activated carbon, comprising the following steps: mixing the oil-containing activated carbon with a surfactant, and then subjecting the mixture to ultrasonic treatment to obtain a first oil-containing activated carbon; extracting the first oil-containing activated carbon with supercritical carbon dioxide to obtain a second oil-containing activated carbon and a first oil component, and recovering the first oil component; treating the second oil-containing activated carbon with microwaves to obtain regenerated activated carbon and a second oil component, and recovering the second oil component. The method of the present application adopts a low-temperature treatment process throughout the entire process to avoid high temperature-induced structural damage to the activated carbon, resulting in a low coking rate and significant energy savings; oil resource recovery is achieved through supercritical carbon dioxide extraction and microwave desorption of the oil component, and no harmful substances are generated during the process; a multi-stage regeneration process can effectively desorb the oil in the oil-containing activated carbon, and the activated carbon adsorption capacity recovery rate after regeneration is high, with low energy consumption.

The invention relates to the field of food processing, and discloses a flavor oil processing technology for improving phenolic nutrient substances, which comprises the following steps: (1) mixing oil seeds with water, and carrying out low-temperature treatment at 20 DEG C or below to obtain wetted oil seeds; (2) stir-frying and squeezing the wetted oil seeds to obtain first crude oil and an oil cake; (3) mixing the oil cake with water, performing heat treatment, and performing oil extraction to obtain second crude oil; and (4) mixing the first crude oil and the second crude oil to obtain the vegetable oil. By adopting the method disclosed by the invention, the content of polyphenol, sterol and flavor substances in the vegetable oil can be further improved.

The utility model discloses an inflatable splint for fixing a broken limb, which comprises a splint outer layer, a magic tape, a splint inner layer and an inflatable hole, a gap is reserved between the splint outer layer and the splint inner layer, and a fixing structure is arranged on the splint outer layer and the splint inner layer. The fixing structure comprises a left fixing plate, a left rotating block, a vertical rod, a connecting block, a right rotating block and a right fixing plate, the left fixing plate is fixed on the outer layer of the clamping plate, and the right fixing plate is fixed on the inner layer of the clamping plate. According to the inflatable splint for fixing the fractured limb, the gap is formed between the inner layer and the outer layer and used for containing an ice bag, when the limb of a wounded person is not completely fractured, low-temperature treatment can be conducted on the wound, hemostasis is conducted on the wounded part in cooperation with a spinning tourniquet arranged at the end opening of the outer layer, and the fixing structure is arranged to fix the inner splint and the outer splint; and the ice bag can be separated from the inner interlayer and the outer interlayer by lifting the vertical rod, so that the ice bag can be comprehensively and uniformly placed or taken out between the inner interlayer and the outer interlayer.

The utility model relates to the field of rice breeding, and discloses a rice breeding germination accelerating device which comprises a breeding box, a breeding box is arranged in an inner cavity of the breeding box, a solar simulator is arranged on the top, close to the breeding box, of the breeding box, a refrigerator is arranged on one side of the inner cavity of the breeding box, and sliding grooves are formed in the two sides of the top of the breeding box. Sliding blocks are installed in the two sliding grooves in a sliding mode, and a connecting pipe is fixedly installed between the two sliding blocks. By means of the breeding box and the refrigerator, the temperature in the breeding box can be controlled to be 0-15 DEG C, the germination rate and quality of seeds can be improved, the yield and quality of rice are improved, the stress resistance of the rice seeds is improved to a certain degree through low-temperature treatment, and the rice seeds cannot be damaged in drought, low-temperature, saline-alkali and other environments. Compared with untreated seeds, the seeds subjected to low-temperature treatment are more suitable and can better grow and develop, and compared with other treatment modes, excessive energy and materials are not needed, so that the cost is lower, and the method is more environment-friendly.

The invention discloses a liriodendron hybrids LhCOLD1 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the LhCOLD1 gene disclosed by the invention is shown as SEQ ID NO. 1, and the amino acid sequence of the expression protein of the LhCOLD1 gene is shown as SEQ ID NO. 2. The method comprises the following steps: constructing an overexpression vector of a hybrid liriodendron LhCOLD1 gene, and transforming the overexpression vector into arabidopsis thaliana and hybrid liriodendron calluses; and cultivating and screening to obtain a transgenic line with improved low-temperature stress resistance. After the LhCOLD1 transgenic arabidopsis thaliana plant is subjected to low-temperature treatment at-10 DEG C for 3 hours and the LhCOLD1 transgenic arabidopsis thaliana plant recovers to grow at room temperature for two weeks, the growth of the LhCOLD1 transgenic After the LhCOLD1 transgenic hybrid liriodendron tulipifera strain is treated at the low temperature of-10 DEG C for 1.5 hours, the influence of low-temperature stress on the growth is small. The result shows that the LhCOLD1 gene enhances the tolerance of the plant to low-temperature stress.

The application discloses a kind of composite mycelium imitation leather materials and preparation method thereof, and relates to the technical field of composite materials.The preparation method comprises the following steps: homogenizing mycelium to form mycelium paste, then mixing the mycelium paste with modified polyvinyl alcohol solution to obtain a mixture, adding glycerol, stirring uniformly, pouring into a mold after the elimination of bubbles, and after low-temperature treatment and drying treatment, the mycelium material is stripped from the mold to obtain the mycelium imitation leather material;The mycelium is the mycelium of polypore family fungi.The preparation method has the advantages of high production efficiency, easy to scale, simple operation, short production cycle, etc.The composite mycelium imitation leather material prepared by the preparation method of the application has good mechanical properties, including high tensile strength, tear strength and flexibility, etc., which can fully meet the requirements of imitation leather products.

The invention discloses a breeding method for promoting flowering phase synchronization of winter wheat and spring wheat, and belongs to the technical field of wheat breeding and cultivation. The breeding method comprises the following steps: disinfection and germination, low-temperature vernalization, transplanting and cultivation, and flowering phase synchronization. The method has the beneficial effects that aiming at the vernalization / low-temperature treatment stages of wheat with different winter and spring properties after germination, environmental conditions such as the photoperiod, the light intensity, the environment temperature and the vernalization / low-temperature treatment duration and environmental conditions such as the photoperiod, the light intensity and the environment temperature in the growth and development stage after vernalization are regulated and controlled; flowering phase encounter of winter wheat and spring wheat is promoted, and the growth cycle is remarkably shortened. According to the method, the flowering phases of the spring wheat and the winter wheat can meet, the bottleneck that the spring wheat and the winter wheat are difficult to hybridize due to ecological distant flowering asynchronism is broken through, and the growth cycle is remarkably shortened compared with a field.

The invention discloses a carbon-based polycyclic aromatic hydrocarbon adsorbent and a preparation method and application thereof.The preparation method comprises the steps that firstly, a mixture of a pretreated coconut shell material and a modifier is subjected to high-temperature treatment to obtain a carbonized material, then the carbonized material is immersed in an activating solution to be subjected to low-temperature treatment, and then filtering, washing to be neutral and drying in the shade at the room temperature are conducted to obtain the carbon-based polycyclic aromatic hydrocarbon adsorbent. Carrying out secondary high-temperature treatment to obtain an activated material; and crushing and sieving the activated material to obtain the carbon-based polycyclic aromatic hydrocarbon adsorbent. The carbon-based polycyclic aromatic hydrocarbon adsorbent has obvious advantages in the selective adsorption and removal process of polycyclic aromatic hydrocarbon, silicon species are introduced in the carbonization process, a microporous structure in a carbon material is destroyed in a pillared mode, then the pore structure of the catalyst is regulated and controlled, the mesoporous structure is richer, and the adsorption selectivity of the polycyclic aromatic hydrocarbon is improved; the adsorbent has high adsorption capacity and adsorption selectivity.

The utility model discloses a decommissioned potting power battery disassembling device and a decommissioned potting power battery disassembling system. The decommissioned potting power battery disassembling device comprises cutting equipment and folding equipment, the cutting equipment comprises a base, a fixed platform, a jacking air cylinder, a limiting piece, a sliding table module, a saw cutting motor and a saw blade. The limiting pieces and the jacking air cylinders are installed on the two sides of the fixed platform respectively, the limiting pieces correspond to the jacking air cylinders in a one-to-one mode, the battery pack side plates are located on the outer side of the platform open groove, and the glue pouring power battery pack module is located on the inner side of the platform open groove; the saw cutting motor drives the saw blade to rotate, and the sliding table module drives the saw blade to move along the platform slot to cut off the side plate of the battery pack; the folding equipment comprises a base, a sliding platform, a linear motor, a portal frame, a pressing oil cylinder and a stretching oil cylinder. By adopting the scheme of the utility model, a sol agent does not need to be filled, pollution to the environment is avoided, low-temperature treatment of the battery module is not needed, and relatively large potential safety hazards to the battery module are avoided.

The invention relates to the technical field of experimental equipment, in particular to Hopkinson torsion bar high and low temperature test equipment, which comprises a temperature adjusting device, a temperature control device, a Hopkinson torsion bar, a Hopkinson torsion bar, a Hopkinson torsion bar, a Hopkinson torsion bar, a Hopkinson torsion bar and a Hopkinson torsion bar, the testing device comprises a fixing assembly and an incident rod which are coaxially arranged, the fixing assembly is provided with a sample fixing groove, the sample fixing groove is formed in the testing space, a first to-be-tested sample can be fixed in the sample fixing groove, a sample fixing part is arranged at the first end of the incident rod, and a second to-be-tested sample can be fixed to the sample fixing part. A mud breaking layer is further arranged between the first to-be-tested sample and the second to-be-tested sample, the first end of the incident rod can drive the second to-be-tested sample to extend into the sample fixing groove, and the first to-be-tested sample rotates while applying pressure towards the fixing assembly to the first to-be-tested sample. Therefore, the problems that the experiment needs to be transferred after being subjected to high-temperature and low-temperature treatment in the friction experiment and the experiment result is influenced are solved.

The invention belongs to the technical field of preparation of nano silicon materials, and particularly relates to a method for preparing nano silicon by etching a silicon-aluminum alloy and a nano silicon material thereof. Comprising the following steps: placing a pretreated silicon-aluminum alloy sample wafer in an etching tank containing a main etching solution, etching under the action of an alternating pulse electric field and ultrasonic-megasonic coupling, and adjusting the pH value to 2.5-3.0 by using acetic acid; quickly washing the silicon-aluminum alloy sample wafer subjected to main etching, putting the silicon-aluminum alloy sample wafer into an electrolytic cell containing a composite buffer solution of oxalic acid and sulfuric acid, and performing electrochemical fine etching under a direct-current constant-voltage electric field; and when the etching current density is reduced to 10% of the initial value, the reaction is terminated, and after low-temperature treatment and low-temperature vacuum drying, the nano silicon material with the residual Al content of 8-12 wt% and the O / Si atomic ratio of 0.15-0.25 is obtained. According to the invention, the problems of out-of-control components, structural damage and serious pollution in the prior art are solved, and the nano silicon material with controllable residual aluminum element and low oxygen-silica ratio can be stably prepared.

The present invention belongs to the technical field of the preparation of electrode materials, and discloses a method for preparing a large-sized carbon electrode with controllable microchannel size and morphology. The steps are as follows: First, the biomass powder is subjected to delignification treatment; then, a mixed suspension of the biomass powder, a crosslinking agent, and a solvent is prepared, and a low-temperature treatment is carried out at different temperature gradients by using a temperature-controlled refrigeration device to control the microchannel size; at the same time, a unidirectional, bidirectional, or non-directional low-temperature treatment method is used by the temperature-controlled refrigeration device to adjust the morphology of the microchannel; finally, a carbonization treatment is carried out. The method of the present invention can prepare a through-type microchannel carbon electrode with different pore sizes and microchannel morphologies by adjusting the preparation process parameters, and is expected to be applied to various fields such as chemical production and environmental protection, so it has broad application prospects.

The invention relates to the field of fermentation product extraction methods, in particular to a method for efficiently extracting intracellular products. According to the method, staged extraction and ultrasound are combined to be applied to intracellular product extraction, and the extraction rate can be obviously increased. After thalli in fermentation liquor are crushed, a non-polar solvent is adopted for first extraction, and ultrasonic short-time low-temperature treatment is adopted in the extraction process, so that the extraction efficiency of a target product can be obviously improved, a polar solvent is added into an extraction phase of the first extraction in second extraction, and ultrasonic short-time low-temperature treatment is performed again, so that the extraction amount of intracellular products is increased; the process does not need complex equipment, is short in operation time consumption and high in extraction efficiency, and is suitable for large-scale industrial production.

This invention relates to the technical field of cryogenic natural gas separation and discloses a pollution-free cryogenic natural gas separation and treatment device, including a treatment tank. A cryogenic tank is fixedly installed at the bottom of the inlet tank. A condenser pipe is installed on the inner wall of the installation groove. A liquid nitrogen pipe is inserted and connected to the upper part of one side of the outer wall of the treatment tank. A pressure sensor is installed on the upper part of one side of the treatment tank, and a temperature sensor is installed in the middle of one side of the treatment tank. In this invention, natural gas is introduced into the inlet tank for accumulation and cryogenic separation. Then, the liquefied liquid and natural gas fall directly into the cryogenic tank below. The condenser pipe is controlled by the compressor to start cooling, so that the cryogenic natural gas is further cryogenically processed. After the temperature sensor detects that the temperature remains constant on the outside, it controls the first and second electromagnetic sealing valves through wires to open them and perform gas-liquid separation, thereby improving the overall effect of natural gas separation of the equipment.

The invention discloses a physical induction method of poplar 2n pollen. The invention uses low temperature as an inducer to induce and treat poplar pollen mother cells to obtain poplar 2n pollen; further, by optimizing the technical conditions for inducing chromosome doubling of poplar pollen by low temperature treatment, the optimal technical parameters such as effective treatment period, treatment temperature and continuous treatment time are screened, and finally high-frequency poplar 2n pollen is obtained, and a technical system for low-temperature induction of poplar pollen chromosome doubling and breeding triploids is established, which greatly improves the ratio of poplar 2n pollen, and the male inflorescence of poplar treated by low temperature induction can shed pollen normally and has high pollen vitality, which is suitable for hybrid pollination of triploids; compared with the current colchicine induction and high temperature induction methods, the low temperature induction of 2n pollen has the advantages of simple operation, low cost, high relative safety, etc., and has theoretical and practical significance in constructing a new and efficient poplar triploid breeding technology system.

The invention relates to the technical field of sewage treatment, and particularly discloses a sewage treatment agent as well as a preparation method and application thereof. The sewage treatment agent comprises modified polyaluminum chloride, a low-temperature flocculant and a plurality of auxiliary components, the modified polyaluminum chloride is obtained by doping nano boron nitride and modifying with a low-temperature modifier, and the low-temperature flocculant is obtained by copolymerization of polyacrylamide and 2-acrylamide-2-methylpropanesulfonic acid; the preparation method mainly comprises the steps of raw material dissolution, mixing reaction, flocculation compounding and the like. The sewage treatment agent can be used for domestic sewage treatment in a low-temperature environment of 0-5 DEG C, has the advantages of excellent low-temperature resistance, high hydrolysis speed, high pollutant removal rate and good stability, and can effectively overcome the defect of poor low-temperature treatment effect of an existing agent.

The application provides a method for improving the adenosine content of Ophiocordyceps sinensis based on metabolic regulation and synergistic induction, wherein a metabolic intervention is performed on the larva and strain complex using a regulating agent during the infection period before low-temperature treatment to form the infected larva; first, deep low-temperature immobilization treatment is performed at 2-5 DEG C for 30-50 days, then culture is performed using a variable-temperature cycle with diurnal temperature difference during the stroma induction period, and mannitol is sprayed onto the surface of the culture medium at the beginning of the step; after harvesting, rapid temperature rising enzyme inactivation and drying treatment is performed on the harvested Ophiocordyceps sinensis, the rapid temperature rising enzyme inactivation and drying treatment comprises increasing the drying temperature from 40-45 DEG C to 60-70 DEG C within 30-45 minutes and maintaining the temperature until the moisture content is less than 10%. The application can solve the problem that the adenosine content is generally low in the existing full artificial cultivation technology, and can make the adenosine content of the full artificial cultivation Ophiocordyceps sinensis reach more than 0.020% (w / v), which is significantly better than the existing artificial cultivation level, and the product morphology is complete and the reproducibility is high.

The present invention relates to a method for promoting the chlorosis and coloration of regreening summer orange fruits, comprising the steps of: subjecting the regreening summer orange fruits to a cold shock treatment; and subjecting the cold-shocked regreening summer oranges to a low-temperature treatment for 5-7 days. The method of the present invention achieves chlorosis and coloration of regreening summer oranges by using only the cold shock and low-temperature treatment methods without the use of chemical reagents. The method is simple to operate, low-cost, safe and effective, and the treatment time is short, controlled within 5-7 days. This method provides theoretical guidance and technical support for improving the color quality of summer orange fruits and increasing the benefits for fruit growers.

This invention provides a method for synergistically preparing sterile liquid formulations using high-energy fluid milling and plant components, relating to the field of traditional Chinese medicine technology. The method involves subjecting medicinal plant raw materials to a first low-temperature treatment using high-energy fluid milling to obtain highly active functional components. These functional components are then mixed with a liquid formulation matrix and subjected to a second low-temperature treatment using high-energy fluid milling, simultaneously achieving homogenization and sterilization. This invention reveals for the first time a significant synergistic sterilization effect between the medicinal plant functional components prepared by a specific process and the high-energy fluid milling treatment. Complete microbial inactivation can be achieved under mild conditions of 120 MPa and below 50°C, with effects far exceeding the simple superposition of a single component and a single physical treatment. This invention provides an integrated homogenization-sterilization method for external preparations and natural beverages that eliminates the need for chemical preservatives and high-temperature sterilization, and has broad applicability in the fields of traditional Chinese medicine and food.

The invention discloses a method for ultra-low temperature preservation and detoxification of pinellia ternata, comprising material culture, loading treatment, vitrification treatment, ultra-low temperature treatment, thawing and warming treatment, unloading treatment and regeneration plant culture. The pinellia ternata vitrification ultra-low temperature preservation technology system established by the invention can be applied to the preservation of pinellia ternata germplasm resources. Compared with field preservation and delayed growth preservation technology, the technology can greatly save manpower and material resources, is easy to operate, is not easily attacked by natural disasters, does not need to transfer plant materials regularly, occupies a small space, has low preservation cost, long preservation time, is genetically stable, and can preserve pinellia ternata germplasm for a long time; the technology can also be applied to the detoxification of pinellia ternata plants. After detoxification of test tube tubers of second-generation diseased plants of pinellia ternata, it was found through experimental verification that the detoxification rate of soybean mosaic virus (SMV) was 85.7%, and the detoxification rate of cucumber mosaic virus (CWV) was 57.1%, and the detoxification effect was better than that of commonly used heat treatment and stem tip detoxification.

The invention relates to the technical field of plant germplasm resource identification, and discloses a method for identifying cold resistance of coffee. The identification method comprises the following steps: performing comprehensive cold resistance evaluation on the conductivity, catalase content, soluble sugar content and soluble protein content of obtained leaves by adopting coffee leaves subjected to low-temperature treatment through a membership function method to obtain cold resistance membership function values Uij of different coffee germplasm resources; and judging the cold resistance of the coffee resource through the obtained cold-resistant membership function value Uij. According to the method, a multi-index comprehensive evaluation system is established according to a membership function method, and the cold resistance of coffee can be effectively identified in the early stage. The identification method is suitable for a technical system for identifying and evaluating the early cold resistance of different coffee resources in the field in the natural cooling process, is matched with the winter field cold resistance identification result of a coffee planting area, and has important significance on utilization of different germplasm resources of coffee, reasonable planning and layout of a planting site and cold resistance breeding.

The invention discloses a rapid propagation rooting culture medium for peony seed tissue culture and a culture method, and belongs to the technical field of plant tissue culture. The rapid propagation rooting culture medium takes 1 / 2 MS as a basic culture medium, and further comprises the following components: ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, monopotassium phosphate, a carbon source, kinetin and a curing agent. By adding kinetin into the culture medium, dependence of peony seed tissue culture seedlings on low-temperature treatment is broken, low-temperature pretreatment necessary in a traditional method is avoided, the peony seed tissue culture process is greatly simplified, the culture period is shortened, the rooting rate and the root strengthening rate are increased, the rooting rate is stabilized to be 95% or above, and the rooting rate is stabilized to be more than 95%. Technical support is provided for establishment of a new peony seed tissue culture and rapid propagation technology, and a material foundation is laid for promotion of peony seed tissue culture and rapid propagation factorization.

The invention provides a high-strength and high-conductivity copper-chromium alloy and a short-process manufacturing method. The high-strength and high-conductivity copper-chromium alloy comprises the following components: 0.2 to 1.0 percent of Cr, 0.05 to 0.30 percent of Ag, 0.01 to 0.20 percent of Ti, 0.01 to 0.20 percent of Si, 0.04 to 0.15 percent of Fe and the balance of copper and inevitable impurities. Compared with the prior art, by adopting the components, the cold rolling machining rate before aging reaches the high machining rate of 90% or above, and the aging treatment temperature timely reaches the temperature required by sufficient precipitation of Cr and the like from a parent phase. According to the method, only one time of aging treatment is carried out after hot rolling, namely cold rolling with the machining rate reaching 90% or above is carried out after hot rolling. And the product is manufactured through final cold rolling and low-temperature treatment, so that the manufacturing process of the product can be shortened. And the tensile strength of the product exceeds 600MPa, and the electric conductivity is greater than 80% IACS.