713results about "Lyases" patented technology

The invention discloses an arginine decarboxylase diaA enzyme mutant and application thereof in preparation of butanediamine, and belongs to the field of bioengineering. According to the invention, rational charge overturning transformation is simultaneously carried out on a pentamer meridian oligomeric interface and a dimer latitudinal oligomeric interface, so that stable assembly and efficient catalysis of the decamer under the condition of neutral to alkaline pH (7.0-9.0) are realized. Wherein positive charges are introduced into a meridian interface to weaken electrostatic repulsion, and negative charges are introduced into a latitudinal interface to enhance dimer compactness and substrate transfer efficiency. The specific enzyme activity of the representative double mutant AdiAD110K / H736E at pH 8.0 is about 35 times that of a wild type, and the representative double mutant AdiAD110K / H736E keeps a complete decamer state in a pH range of 7.0-9.0. The yield of butanediamine is up to 145.9 g / L under the whole-cell catalysis of the mutant.

The invention discloses a rational copolymerization strategy for improving the stability of an oligomeric structure of acid-induced high-order oligomeric decarboxylase AdiA in a neutral to alkaline environment and application of the rational copolymerization strategy, and belongs to the field of bioengineering. According to the strategy, rational charge overturning transformation is carried out on a pentamer radial oligomeric interface and a dimer weft-wise oligomeric interface at the same time, and stable assembly and efficient catalysis of a decamer under the condition that the pH value is from 7.0 to 9.0 from neutral to alkaline are achieved. Wherein positive charges are introduced into a meridian interface to weaken electrostatic repulsion, and negative charges are introduced into a latitudinal interface to enhance dimer compactness and substrate transfer efficiency. The specific enzyme activity of the representative double mutant AdiAD471K / E467K / H736E is 45.5 times that of a wild type when the pH value is 8.0, and the representative double mutant AdiAD471K / E467K / H736E keeps a complete decamer state when the pH value is 7.0-9.0. The yield of butanediamine is up to 156.5 g / L by using the mutant to catalyze whole cells.

The invention discloses a high-efficiency lysine decarboxylase mutant and an application of the high-efficiency lysine decarboxylase mutant in synthesis of pentamethylene diamine. A series of mutants including a single mutant (such as N218G, T222V, C244P and the like), a double mutant and a three mutant are obtained by carrying out multi-site mutation on wild type lysine decarboxylase (SEQ ID NO.2) from Klebsiella grimontii. The catalytic efficiency of the mutant is remarkably improved, and the activity of the mutant is improved by 5.0-10.2 times compared with that of a wild type enzyme. According to the method disclosed by the invention, the substrate conversion rate of 99% or above can be realized within 2 hours through an optimized process of constructing an expression vector and a genetically engineered bacterium, inducing expression of lysine decarboxylase and synthesizing 1, 5-pentanediamine by utilizing a whole-cell catalysis technology. Compared with a traditional chemical method and a natural enzyme method, the method disclosed by the invention has the advantages of simplified process, high catalytic efficiency, low production cost and the like, and is suitable for producing bio-based 1, 5-pentamethylene diamine and nylon 56 salt.

The invention provides a kit for rapidly detecting MRSA and drug-resistant gene nucleic acid POCT of the MRSA based on a phage lyase mutant. The kit comprises nucleic acid extraction and purification and triple PCR rapid amplification detection reagents. The staphylococcus aureus bacteriophage lyase mutant nucleic acid release liquid containing the amino acid sequence as shown in SEQ ID NO: 1 is adopted, wall breaking can be rapidly conducted on staphylococcus aureus, and nucleic acid is released. The effect of the nucleic acid release liquid acting for 5 minutes is equivalent to or even better than that of the lysozyme acting for 60 minutes; according to the present invention, with the combination of the MRSA and drug-resistant gene triple PCR rapid amplification detection reagent, the total detection time is only 35 min, the time is saved by 55 min or more than 55 min compared with the lysozyme method, and the MRSA and drug-resistant gene nucleic acid POCT rapid detection can be achieved;

The present invention provides a 4-vinylguaiacol oxygenase mutant for synthesizing vanillin, and an engineered bacterium thereof. The 4-vinylguaiacol oxygenase mutant is an enzyme having the sequence as shown in SEQ ID NO. 1, which exhibits a double-site mutation of D27P / T216M, D27P / M351F, D27P / Y156D, M351F / Y156D, T216M / Y156D or T216M / M351F, or a single-site mutation of T216M, T216L, S280M, G307P or M351F, and can be used for catalyzing 4-vinylguaiacol to generate vanillin.

The invention relates to an Escherichia coli engineering strain for producing 2, 3-pentanediol as well as a construction method and application of the Escherichia coli engineering strain, and the method comprises the following steps: constructing recombinant plasmids pACYC-IlvA *-ThrA * BC for expressing an IlvA * gene of threonine deaminase, a ThrA gene of asparaginic acid kinase I, a Thr B gene of homoserine kinase and a ThrC gene of threonine synthetase; the method comprises the following steps: constructing a recombinant plasmid pRSF-Ilv2C-Aro10 for expressing an Ilv2C gene of acetohydroxy acid synthetase and an Aro10 gene of propiophenone dehydrogenase; and chemically converting the recombinant plasmid pACYC-IlvA *-ThrA * BC and the recombinant plasmid pRSF-Ilv2C-Aro10, and transferring the recombinant plasmid pACYC-IlvA *-ThrA * BC and the recombinant plasmid pRSF-Ilv2C-Aro10 into escherichia coli so as to obtain the escherichia coli engineering strain for producing the 2, 3-pentanediol. According to the escherichia coli engineering strain, the biosynthesis of 1.61 g / L of 2, 3-pentanediol is realized by using L-threonine and 40 g / L of glucose.

The invention provides a saccharomyces cerevisiae engineering strain HYB05 capable of releasing astaxanthin in a controllable self-splitting manner. The saccharomyces cerevisiae engineering strain HYB05 is constructed by taking a basic strain for producing astaxanthin as a starting strain, introducing a coding sequence comprising cell wall lyase Lyc, a coding sequence of a signal peptide mutant F8A and a recombinant nucleic acid construct for expressing a regulatory element, and knocking out an SSD1 gene to relieve a translation inhibition effect. The cell wall lyase Lyc is found and disclosed by the inventor for the first time, and the amino acid sequence is shown as SEQ ID NO: 6; the amino acid sequence of the signal peptide mutant F8A is as shown in SEQ ID NO: 8. According to the engineering strain HYB05, by introducing the recombinant nucleic acid construct, the activation of the catalytic activity of cell wall lyase under specific conditions is realized, so that the cell wall of the saccharomyces cerevisiae is broken, and the astaxanthin synthesized by the saccharomyces cerevisiae is released. Compared with the prior art, the astaxanthin is produced through fermentation of the strain, the extraction steps are simplified, extraction of the astaxanthin in the yeast is achieved to the maximum extent, the yield of the astaxanthin is remarkably increased, and the technical effect is remarkable.

The invention provides a phaffia rhodozyma mutant strain for high yield of astaxanthin as well as a screening method and application of the phaffia rhodozyma mutant strain, belongs to the technical field of fermentation microorganism screening, and can solve the problems of low astaxanthin yield, high cost and low growth temperature of wild type phaffia rhodozyma existing in synthesis of astaxanthin from a phaffia rhodozyma strain. The invention provides a phaffia rhodozyma mutant strain capable of producing astaxanthin at high yield, the phaffia rhodozyma mutant strain is a phaffia rhodozyma AA0529 mutant strain, the phaffia rhodozyma AA0529 mutant strain is preserved in China Center for Type Culture Collection in Wuhan, Hubei on September 7, 2023, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M20231637. According to the method, the yield of the astaxanthin can be increased, and the yield of the astaxanthin is increased to 3.3 mg / g at 26 DEG C.

The invention belongs to the technical field of gene engineering, and discloses recombinant yarrowia lipolytica as well as a construction method and application thereof. The recombinant yarrowia lipolytica PGA1 is obtained by carrying out heterologous expression on an arabidopsis thaliana inositol oxygenase gene and a pseudomonas syringae uronic acid dehydrogenase gene in the yarrowia lipolytica by utilizing a genetic engineering technical means. The recombinant yarrowia lipolytica PGA10 capable of efficiently synthesizing glucaric acid (GA) is finally obtained by further modifying six genes of related metabolic pathways of the recombinant yarrowia lipolytica PGA1. The GA yield of the PGA10 reaches 2233.24 mg / L and is 66.27 times that of PGA1, and the effect is remarkable. The invention not only constructs a GA synthesis method, but also defines the influence of related genes such as opi1 on GA synthesis, and provides reference for the research of heterologous synthesis of GA by microorganisms.

The invention discloses a method for biological high-density synthesis of retinaldehyde and a synthetic strain thereof. The synthetic strain is obtained by expressing ATP citrate lyase ACL, acetyl-CoA synthase ACS, choline kinase CK, inositol polyphosphate kinase IPK and vitreoscilla hemoglobin VHb in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified bacterial strain PP-B2C. The retinaldehyde production performance of the recombinant strain is verified on the basis of a precursor supply pathway, an IUP pathway and a dissolved oxygen level, and the retinaldehyde production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of retinaldehyde can be better promoted, and the retinaldehyde can be efficiently synthesized by the engineering strain by utilizing glucose and methanol through amplification fermentation of a 5L fermentation tank, so that the recombinant pichia pastoris is beneficial to industrial production.

The invention discloses a bacteriophage lyase and application thereof, and particularly relates to a bacteriophage lyase with an amino acid sequence as shown in SEQ INNO.2. The bacteriophage lyase can inhibit the growth of escherichia coli, staphylococcus and salmonella, can be used as an antibacterial substance in splitting gram-negative bacteria and can be used for preparing drugs for resisting gram-negative bacteria. The lyase can be used independently or compounded with other substances, the use concentration of antibiotics can be reduced by combining the lyase with the antibiotics, toxic and side effects (such as renal toxicity of polymyxin) of drugs are reduced, and meanwhile, generation of bacterial drug resistance is delayed. After the lyase and the chitosan are combined for use, remarkable antibacterial activity is generated, the antibacterial effect can be achieved without pre-treatment on bacteria, and the practicability is improved.

The invention discloses a method for preparing butanediamine and spermidine from whole cells, and belongs to the field of bioengineering. According to a rational copolymerization modification strategy, a meridian interface and a latitudinal channel interface of arginine decarboxylase are subjected to rational modification respectively or simultaneously, and a series of mutants are obtained. The optimal mutant AdiA H729D / E467K / H736E, which is subjected to double-interface synergistic modification, disclosed by the invention, keeps high activity in the whole neutral-alkaline range of pH (Potential of Hydrogen) of 7.0 to 9.0. The optimal pH of the arginine decarboxylase mutant is increased, the enzyme activity stability in a neutral pH range is improved, the arginine decarboxylase mutant is more suitable for the condition requirements of industrial microbial fermentation, and a foundation is laid for efficient synthesis of butanediamine. The spermidine yield is up to 153.3 mg / L by using the mutant to catalyze the whole cell.

The invention provides low-temperature alginate lyase and application thereof, namely alginate lyase with low-temperature catalytic activity, which is separated from marine-derived cold monad bacteria, and the amino acid sequence of the alginate lyase is SEQ ID NO: 1; the amino acid sequence of the alginate lyase after the signal peptide is removed is SEQ ID NO: 3. The algin lyase Alg3889 provided by the invention has the optimal reaction temperature of about 10-16 DEG C, has good temperature stability, and still keeps more than 70% of enzyme activity after being incubated at 0-70 DEG C for 1 hour; the optimum pH value of the strain is 8.0, after the strain is incubated in a buffer system with the pH value of 3.0-10.6 for 12 h, about 90% of the highest enzyme activity can be kept, and the strain has high acid resistance and alkali resistance. The degradation product of the alginate oligosaccharide is alginate oligosaccharide with the polymerization degree of 2-6, and the alginate oligosaccharide has high application value.

The invention provides a genetically engineered bacterium for producing astaxanthin, and the genetically engineered bacterium takes Yarrowia lipolytica Po1f as an original strain and contains a GGS gene, an HMGR gene, a beta-carotene synthesis gene, a beta-carotene ketolase gene and a beta-carotene hydroxylase gene. The invention further provides the Yarrowia lipolytica MC247, and the preservation number of the Yarrowia lipolytica MC247 is CGMCC NO.29699. According to the Yarrowia lipolytica MC247, the high-yield astaxanthin is obtained, a new technical scheme is provided for efficient and green manufacturing of the astaxanthin, and industrial production of the astaxanthin is facilitated.

Herbicide-resistant rice plants, isolated polynucleotides that encode herbicide resistant and wild-type acetohydroxy-acid synthase large subunit 1 (AHASL1) polypeptides, and the amino acid sequences of these polypeptides, are described. Expression cassettes and transformation vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, are described. Methods of using the polynucleotides to enhance the resistance of plants to imidazolinone herbicides, and methods for controlling weeds in the vicinity of herbicide-resistant plants are also described.

Described herein are biocatalytic methods of producing terpene degradation products useful as starting material for the production of perfumery ingredients, such as, for example, ambrox. In particular novel terpene degrading polypeptides (enal-cleaving polypeptides) and novel peptides converting terpenes compounds to oxygenated derivatives (oxygenases) and mutants and variants derived therefrom are described which may be applied in novel types of fully enzymatic multistep degradation pathways allowing the controlled, stepwise conversion and degradation of linear or cyclic terpene substrates. Said novel biosynthetic strategies allow the fully biochemical synthesis of valuable terpene-derived compounds, like for example manooloxy or gamma ambrol. Also described herein are recombinant host organisms carrying the required set of genetic information for the functional expression of the set of enzymes necessary for catalyzing the combination of enzymatic conversion and degradation steps.

The invention belongs to the field of metabolic engineering, and discloses a genetically engineered bacterium for producing myo-inositol as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as an original strain, and is obtained by performing the following gene editing on a genome of the escherichia coli: non-expressed lactose operon repressor protein, glucose phosphate dehydrogenase, acetokinase, glucose phosphate isomerase and glycerol repressor protein; and overexpressing inositol-1-phosphate synthase, inositol monophosphate, glucokinase, glucose permease and glycerol kinase. Compared with the prior art, the genetically engineered bacterium disclosed by the invention has remarkable advantages in the aspects of key enzyme expression rate, metabolism specificity, fermentation period and the like, and an efficient, stable, economical and feasible solution is provided for industrial production of myo-inositol.

The invention discloses a Pickering IL emulsion microreactor constructed on the basis of temperature-sensitive double-enzyme Janus spheres and application of the Pickering IL emulsion microreactor to enhanced catalysis of conversion of CO2 into formic acid. A Janus emulsion template method is combined with a photocuring technology to synthesize a nano-scale dual-functional temperature-sensitive Janus sphere, one half of the nano-scale dual-functional temperature-sensitive Janus sphere is a hydrophilic hydrocarbon polymer, the other half of the nano-scale dual-functional temperature-sensitive Janus sphere is a hydrophobic fluorocarbon polymer, and rapid inversion of a Pickering emulsion can be realized by utilizing temperature sensitivity. The method comprises the following steps: preparing a Janus particle hydrophilic / hydrophobic hemisphere, selectively immobilizing formate dehydrogenase FDH and carbonic anhydrase CA on the Janus particle hydrophilic / hydrophobic hemisphere to obtain a temperature-sensitive FDH-Janus-CA composite sphere, introducing a fluorine-functionalized ionic liquid to regulate and control the internal microenvironment of a microreactor, and constructing a Pickering IL emulsion microreactor. The CO2 absorption capacity is increased by 47 times compared with that of a pure water system, the formic acid yield reaches 27.5 mM and is higher than the maximum value 22.8 mM of current enzyme electrocatalysis, the storage and catalysis stability is good, and an efficient and sustainable scheme is provided for CO2 recycling.

The invention belongs to the technical field of natural product extraction and enzyme engineering, and particularly relates to a method for extracting red pomelo polyphenol based on an enzyme method. The method aims at solving the problems of high cost and the like caused by easy inactivation and difficult recovery of enzymes in most red pomelo extraction processes. Waste such as pericarp and pomelo seeds generated by red pomelo processing are used as carbon sources, a biochar material with a hierarchical porous structure is prepared through programmed heating carbonization activation, pectinase, cellulase and naringinase are immobilized by adopting a spatial zoning sequential immobilization strategy and then react with the red pomelo, and the biochar material with the hierarchical porous structure is prepared. The immobilized enzyme is rapidly recovered through magnetic separation to realize repeated utilization of the enzyme, and finally red pomelo polyphenol powder is obtained through multi-step treatment, so that recycling is realized, and the yield of polyphenol extracted by an enzyme method is improved.

The embodiment of the invention provides a tyrosine ammonia lyase mutant and application thereof in synthesis of p-coumaric acid. According to the embodiment of the invention, an amino acid sequence as shown in SEQ ID NO.2 is taken as a template, a key active site of the template is subjected to mutation transformation, a mutant library is constructed, and a series of mutants with remarkably improved catalytic activity and / or conversion rate are obtained through screening; the tyrosine ammonia lyase mutant provided by the embodiment of the invention can selectively catalyze L-tyrosine to generate p-coumaric acid, has excellent yield and / or higher yield, and has outstanding industrial application prospects in efficient and green synthesis of p-coumaric acid.

The invention discloses yarrowia lipolytica with high yield of beta-carotene as well as a construction method and application of the yarrowia lipolytica. According to the yarrowia lipolytica engineering bacterium, geranyl diphosphate synthase gene xdGPS, phytoene dehydrogenase gene CarB, phytoene cyclization / phytoene synthesis bifunctional enzyme mutant gene GarRPY27R, acetyl-coenzyme A synthetase mutant gene ACSL641P and pyruvate ferredoxin oxidoreductase gene nifJ are integrated and expressed on a chromosome, and the yarrowia lipolytica engineering bacterium is obtained. Meanwhile, a 3-hydroxy-3-methylglutaryl CoA reductase gene HMGR (3-hydroxy-3-methylglutaryl CoA reductase) derived from the yarrowia lipolytica strain is subjected to overexpression; the yield of the beta-carotene is increased to 13.186 g / L, and the production efficiency is 0.14 g / L / h.

A genetically modified yeast cell, wherein the yeast cell comprises a genetic modification comprising overexpression of yeast gene encoding porphobilinogen deaminase (HEM3), the HEM3 gene having at least 80% identity with SEQ ID No. 7. The genome of the modified yeast cell further comprises one or more genetic modifications in one or more genes selected from: genes coding for heme-dependent repressor of hypoxic genes (ROX1), genes coding for heme oxygenase (HMX1), genes coding for a receptor for vacuolar proteases (VPS10), and genes coding for vacuolar proteinase (PEP4), the one or more genetic modifications being such that expression of a polypeptide from such a gene is reduced or disrupted or the polypeptide expressed is non-functional.

The invention relates to the technical field of bioengineering, in particular to a process for continuously producing nicotine based on immobilized enzyme. The immobilized microbial agent comprises a composite carrier, an engineering strain and an immobilized auxiliary agent, in the process for continuously producing nicotine based on immobilized enzyme, amine oxidase of an engineering strain is subjected to site-specific modification, so that spatial structure deformation when the amine oxidase is combined with a carrier is reduced, and a three-dimensional network structure of hydroxyethyl chitosan-guar gum composite microspheres in the composite carrier is matched to provide an adaptive microenvironment; the activity loss of enzyme molecules caused by steric hindrance or structural change is reduced; in the immobilization auxiliary agent, gallic acid and an enzyme molecule sulfydryl form a coordinate bond to maintain an active center conformation, glutaraldehyde and polyethyleneimine enhance the binding stability through a crosslinking effect and charge regulation, and the maintaining effect of enzyme activity during continuous production is improved, so that the enzyme activity loss in the immobilization process is reduced, and the immobilization efficiency is improved. The stability of the enzyme in long-term continuous reaction is enhanced.

The invention provides a method for increasing the yield of L-isoleucine. Comprising the steps that an engineering strain containing an expression plasmid vector or an expression plasmid vector combination is cultured, the vector or combination comprises a first group of polynucleotides and a second group of polynucleotides, and the first group of polynucleotides comprise at least one polynucleotide encoding L-isoleucine synthetic pathway enzyme; the second group of polynucleotides comprising at least one polynucleotide encoding an enzyme that modulates the balance of NAD + / NADH and NADP + / NADPH; and a backbone plasmid capable of autonomously replicating in a host cell. According to the invention, the yield of L-isoleucine and the conversion rate of glucose are improved.

The invention discloses a high-yield and high-activity pear protoplast extraction method based on light shielding and enzymolysis optimization, which comprises the following steps: by taking pear tissue culture seedling leaves growing for 30-40 days or field pear tender leaves as an extraction material, carrying out enzymolysis treatment and protoplast purification by adopting enzymatic hydrolysate to obtain protoplast; before enzymolysis of the pear tissue culture seedlings, the whole pear tissue culture seedlings are placed in a dark environment and subjected to dark pretreatment at 25 + / -2 DEG C for 48-72 hours. According to the method, the yield of the field pear leaf and tissue culture seedling pear leaf protoplast can be increased by more than or equal to 2 times (the highest protoplast yield is greater than or equal to 3.0 * 10 / g FW), the activity is increased to be greater than or equal to 95%, and the technical bottleneck of the existing patent is remarkably broken through. The method has the advantages of high yield, high activity, wide application range and the like, can provide a high-quality protoplast material for pear genetic regeneration, gene editing, single cell transcriptome analysis and metabonomics research, and has wide scientific research and industrial application potential.

The invention belongs to the field of plant tissue culture, and particularly relates to a method for promoting accumulation of camptothecin by overexpressing tryptophan synthetase. The invention provides a method for promoting camptothecin accumulation of camptotheca acuminate cells, which comprises the following steps: constructing camptotheca acuminate cells overexpressing tryptophan synthetase genes, performing tissue culture amplification, and extracting camptothecin from the amplified camptotheca acuminate cells. The method for overexpressing the tryptophan synthetase gene is superior to a method for directly adding tryptophan into a culture medium, and the camptothecin yield can be increased by 2.33 times.

This invention relates to the field of low-temperature fermentation formulation technology. The invention provides a low-temperature resistant compound bacterial enzyme formulation, its preparation method, and its application in the co-fermentation of straw and manure. The compound bacterial enzyme formulation comprises the following raw materials in parts by weight: 8-10 parts of compound bacterial agent, 1-1.4 parts of compound enzyme agent, and 88.6-91 parts of excipients. The compound bacterial enzyme formulation of this invention can ferment straw and manure under conventional conditions as well as at low temperatures of around 15°C, effectively meeting the fermentation requirements in low-temperature regions. By combining the excipients with the bacteria and enzymes, substances and nutrients during the fermentation process can be effectively adsorbed, avoiding acid inhibition, and providing attachment sites for the bacteria and enzymes, thus prolonging their activity.

The invention belongs to the technical field of enzyme gene engineering, and particularly relates to a methionine gamma-lyase mutant with improved enzyme activity and application thereof. The methionine gamma-lyase mutant CqMGLV160I / V265I with high activity is obtained by taking methionine gamma-lyase (CqMGL) which is derived from Calorama quimayensis and of which cysteine residues at 116 sites are replaced by histidine residues as a parent and carrying out site-specific mutagenesis on amino acid residues at key sites through transformation. The methionine gamma-lyase mutant CqMGLV160I / V265I with high activity can be obtained by using the methionine gamma-lyase mutant CqMGLV160I / V265I as the parent. By adopting the CqMGLV160I / V265I mutant disclosed by the invention, the conversion rate of enzyme catalysis can be improved, allicin is efficiently synthesized, and the mutant is successfully applied to the aspects of food, feed, chemical industry, medicine preparation and the like.

This invention discloses a method for producing p-hydroxyphenylethanol. The method involves constructing genetically engineered bacteria capable of expressing L-amino acid deaminase, 4-hydroxymandelic acid synthase, glycolaldehyde dehydrogenase, lactaldehyde reductase, propylene glycol dehydratase, and alcohol dehydrogenase, followed by whole-cell transformation to synthesize p-hydroxyphenylethanol. L-tyrosine is widely available, has low toxicity, and is simple and inexpensive to prepare, making it an ideal substrate. The transformation process does not require the addition of expensive coenzymes, and the enzymes selected in this invention possess advantages such as high activity and strong optical specificity. Therefore, using the engineered bacteria of this invention to produce p-hydroxyphenylethanol is highly efficient, environmentally friendly, and low-cost, showing promising prospects for industrial application.

The invention relates to the technical field of genetic engineering, in particular to a recombinant microorganism and application thereof in preparation of glycine. The application comprises the following steps: producing glycine by adopting the recombinant microorganism; the recombinant microorganism comprises an enzyme combination and / or a nucleic acid molecule combination. The enzyme combination comprises threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase; the threonine aldolase comprises an amino acid sequence as shown in SEQ ID NO. 1. The nucleic acid molecule combination is used for encoding the enzyme combination. According to the invention, threonine aldolase, ethanol dehydrogenase and isopropanol dehydrogenase are converted into microorganisms, and the constructed recombinant microorganisms can be used for decomposing threonine to produce glycine; and a specific system formed by specific isopropanol dehydrogenase, ethanol dehydrogenase and isopropanol dehydrogenase can also have a relatively high conversion rate under the condition of no addition of pyridoxal phosphate (PLP), and has important significance and economic value.