185 results about "Coumaric acid" patented technology

The embodiment of the invention provides a tyrosine ammonia lyase mutant and application thereof in synthesis of p-coumaric acid. The mutant is subjected to any one of the following amino acid mutations on an amino acid sequence as shown in SEQ ID NO. 2: F497I, F497I + L248M, F497I + L248M + M4T, and L248M + F497I + M4T + V482L. According to the embodiment of the invention, an amino acid sequence as shown in SEQ ID NO. 2 is taken as a template, a key active site of the template is subjected to mutation transformation, a mutant library is constructed, and a series of mutants with remarkably improved catalytic activity and / or conversion rate are screened; the tyrosine ammonia lyase mutant provided by the embodiment of the invention can selectively catalyze L-tyrosine to generate p-coumaric acid, and has excellent yield and / or higher yield.

The embodiment of the invention provides a method for obtaining a tyrosine ammonialyase mutant based on high-throughput screening, the tyrosine ammonialyase mutant and application of the tyrosine ammonialyase mutant. The mutant is subjected to any one of the following amino acid mutations on an amino acid sequence as shown in SEQ ID NO.2: A245G + A247T, and D192V + A245G + A247T. According to the embodiment of the invention, an amino acid sequence as shown in SEQ ID NO.2 is taken as a template (basis), a key active site of the template is subjected to mutation transformation, a mutant library is constructed, and a series of mutants with remarkably improved catalytic activity and / or conversion rate are screened; the tyrosine ammonia lyase mutant provided by the embodiment of the invention can selectively catalyze L-tyrosine to generate p-coumaric acid, and has excellent yield and / or higher yield, which indicates that the mutant has more significant application prospects in catalysis of L-tyrosine to generate p-coumaric acid.

The invention discloses a Sarcabomyces lipolytica strain for synthesizing (2S)-naringenin and application of the Sarcabomyces lipolytica strain, and belongs to the technical field of engineering and bioengineering. According to the invention, a key enzyme CHS is subjected to semi-rational transformation, so that the yield of the strain (2S)-naringenin expressing the mutant V196A / T132C is increased by 60%. According to the method, the modified CHS is further integrated into a yeast genome, conversion of p-coumaric acid to (2S)-naringenin is promoted by strengthening a shikimic acid pathway and enhancing supply of cofactors, so that the yield of the (2S)-naringenin reaches 776mg / L after the constructed strain is fermented in a 24-pore plate for 96 hours, and the yield of the constructed strain can reach 8.65 g / L after the strain is fermented in a 5L fermentation tank; the method lays a foundation for the subsequent biosynthesis of flavonoid compounds, and has potential value and significance for the development of synthetic biology.

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The embodiment of the invention provides a recombinant pichia pastoris strain as well as a construction method and application thereof. The construction method comprises the following steps: by taking a pichia pastoris strain as a host strain, constructing a dual-synthesis path of phenylalanine ammonialyase and tyrosine ammonialyase in the host strain to obtain a first engineering strain; removing chorismic acid feedback inhibition of the first engineering strain based on metabolic engineering to obtain a second engineering strain; optimizing an aromatic amino acid synthesis route of the second engineering strain to obtain a third engineering strain; knocking out a branch metabolic pathway of the third engineering strain to obtain a fourth engineering strain; increasing precursor supply of the fourth engineering strain to obtain a recombinant pichia pastoris strain; according to the construction method provided by the embodiment of the invention, the synthesis path of p-coumaric acid can be systematically optimized, the recombinant pichia pastoris strain for efficiently producing p-coumaric acid by taking methanol as a carbon source is constructed, and compared with a host strain, the recombinant pichia pastoris strain has the advantage that the yield of p-coumaric acid is remarkably increased.

The invention provides a preparation method of Keemun camellia seed fermented oil, and belongs to the technical field of vegetable oil production. The preparation method comprises the following steps: S1, preparing recombinant saccharomycetes; s2, jointly fermenting the antarctic torulopsis, the recombinant saccharomycetes and the Keemun camellia seed oil; s3, separating an oil phase from a water phase, and taking the oil phase; and S4, dehydrating the oil phase to obtain the Keemun camellia seed fermented oil. Preferably, according to the preparation method of the recombinant saccharomycetes in the step S1, phenylalanine deaminase, cinnamate hydroxylase, 4-coumaric acid CoA ligase, chalcone synthetase, chalcone reductase and peroxidase are expressed in saccharomyces cerevisiae. According to the Keemun camellia seed fermented oil prepared by the method disclosed by the invention, the characteristic components of the Keemun camellia seed oil can be remarkably improved, the antioxidant capacity of the Keemun camellia seed oil is enhanced while the skin feeling of the Keemun camellia seed oil is improved, and the application value of the Keemun camellia seed oil in cosmetics is improved.

The invention discloses a p-coumaric acid biosensor and application thereof, and the p-coumaric acid biosensor is characterized in that a coding gene of a CarR mutant, a Plpp1.6 promoter, a coding gene of a green fluorescent protein and a Pcar promoter mutant for controlling the expression of the green fluorescent protein are located on an expression vector pTrc99a; the biosensor provided by the invention realizes real-time sensitive monitoring of the concentration of coumaric acid by a recombinant escherichia coli strain containing the coumaric acid biosensor, can be combined with a high-throughput mutagenesis and screening system, and is beneficial to rapid and efficient screening of high-yield engineering strains of coumaric acid; and a technical guarantee is provided for production of p-coumaric acid and derivatives by efficient fermentation of microorganisms. The p-coumaric acid biosensor based on transcriptional regulatory factors is constructed, and a new strategy is provided for high-throughput screening.

The invention relates to the technical field of gene engineering and recombinase catalysis engineering, in particular to a recombinant strain for expressing phenolic acid decarboxylase as well as a construction method and application of the recombinant strain. According to the invention, a recombinant strain for expressing the phenolic acid decarboxylase PADCs is constructed by heterologous expression of a coding gene PADCs of the phenolic acid decarboxylase. The enzyme liquid of the recombinant strain for expressing the phenolic acid decarboxylase has remarkable catalytic performance, p-coumaric acid can be used as a substrate, the recombinant strain is used for inducing expression, the purified enzyme liquid is used for catalyzing decarboxylation reaction of p-coumaric acid, and p-hydroxystyrene is synthesized; meanwhile, the phenolic acid decarboxylase PADCs has a wide substrate spectrum, the 4-vinylphenol compound can be used as a substrate, bicarbonate is used as a carboxyl source, the freeze-dried whole cell of the recombinant strain is utilized to catalyze the 4-vinylphenol compound to carry out regioselective beta-carboxylation reaction, a series of phenolic acids with biological activity are synthesized by an enzymic method, and meanwhile CO2 is immobilized; and a huge application potential is provided for the utilization of CO2.

The invention discloses a phenolic acid inhibitor and a use method and application thereof.The phenolic acid inhibitor comprises a component A. The component A comprises phenolic acid, polyethylene glycol and a phosphate buffer solution, and the phenolic acid is one or more of cumaric acid, kojic acid, chlorogenic acid, gallic acid and ferulic acid; the phenolic acid inhibitor is prepared from the following raw materials in parts by weight: 1 to 3 parts of phenolic acid, 0.1 to 0.3 part of polyethylene glycol and 8 to 12 parts of phosphate buffer solution, and the phenolic acid is two or three of coumaric acid, chlorogenic acid and ferulic acid. The PPO inhibition activity of the phenolic acid inhibitor can reach 70%-80%, the quality of fruits is well maintained, and browning caused by fruit processing is delayed.

The invention relates to the field of gene engineering, in particular to a phenolic acid decarboxylase mutant and application thereof. According to the invention, tryptophan at the 30th site of phenolic acid decarboxylase PADCs from Clostridium sp.DSM 8431 is mutated into leucine, and tyrosine at the 136th site of the phenolic acid decarboxylase PADCs is mutated into tryptophan, so that the phenolic acid decarboxylase mutant is obtained. The phenolic acid decarboxylase mutant can catalyze decarboxylation reaction of p-coumaric acid to synthesize p-hydroxystyrene; and hydrocarbonate can also be used as a carboxyl source to catalyze carboxylation reaction of p-hydroxystyrene to synthesize p-coumaric acid. The phenolic acid decarboxylase PADCs has the enzyme activity characteristics of excellent temperature stability and acetonitrile stability, still retains more than 80% of decarboxylase activity after being incubated for 2 hours at 55 DEG C, still retains about 36% of decarboxylase activity after being incubated for 2 hours in acetonitrile with the volume fraction of 50%, can remarkably improve the yield of p-coumaric acid, can reach 13.05 mM when the substrate concentration is 80 mM, and is improved by 74% compared with original phenolic acid decarboxylase PADCs; the method has a wide industrial application prospect and CO2 fixation potential.

The present invention relates to the field of biotechnology, and particularly to the application of recombinant Saccharomyces cerevisiae in the production of homopterocarpin using p-coumaric acid. The present invention provides an engineered Saccharomyces cerevisiae capable of producing homopterocarpin using p-coumaric acid. Through strategies such as artificial pathway construction, pathway optimization, rational design of enzymes, and fermentation process optimization, the present invention obtains an engineered Saccharomyces cerevisiae capable of producing homopterocarpin using p-coumaric acid. Experiments show that its homopterocarpin yield is 1.005 mmol / l and the conversion rate is 65.8%. In large-scale production in a 5L fermenter, the strain of the present invention can utilize 15.2 mmol / l of p-coumaric acid to produce 3.2 mmol / l of homopterocarpin, and the conversion rate is 21.1%.

The invention provides a method for judging a processing end point of fried rice sprouts and a processing technology of the fried rice sprouts, and the method for judging the processing end point of the fried rice sprouts comprises the following steps: (1) mixing the fried rice sprouts with different frying time and an alcoholic solution, and carrying out ultrasonic extraction to obtain a series of test solution; (2) carrying out liquid chromatography detection on the series of test solutions, calculating peak area ratios of 4-coumaric acid to 5-hydroxymethylfurfural in the fried rice sprouts at different stir-frying times according to detection results, and taking a time period corresponding to a stable peak area ratio range between ratio mutation points as stir-frying time required for reaching a processing end point, and judging the processing end point of the fried rice sprouts. The method provided by the invention can objectively, accurately and clearly judge the processing end point of the fried rice sprouts, fills the blank of judgment of the processing end point of the fried rice sprouts, makes up for the situation of misjudgment caused by evaluation of the processing end point only by colors, realizes monitoring of the processing process of the fried rice sprouts, and can effectively guide the processing technology of the fried rice sprouts.

The application provides a recombinant Pichia pastoris strain and a construction method and application thereof. The construction method comprises the following steps: taking a Pichia pastoris strain as a host strain, constructing a double synthesis pathway of phenylalanine ammonia lyase and tyrosine ammonia lyase in the host strain to obtain a first engineering strain; relieving the branch acid feedback inhibition of the first engineering strain based on metabolic engineering to obtain a second engineering strain; optimizing the aromatic amino acid synthesis pathway of the second engineering strain to obtain a third engineering strain; knocking out the branch metabolic pathway of the third engineering strain to obtain a fourth engineering strain; increasing the precursor supply of the fourth engineering strain to obtain the recombinant Pichia pastoris strain. The construction method can systematically optimize the synthesis path of p-coumaric acid, and the recombinant Pichia pastoris strain for efficiently producing p-coumaric acid with methanol as a carbon source is constructed. Compared with the host strain, the yield of p-coumaric acid of the recombinant Pichia pastoris strain is significantly improved.

The invention discloses a method for producing p-coumaric acid through double-enzyme cascade catalysis, which comprises the following steps: taking phenol, pyruvic acid and ammonium chloride as substrates, and carrying out cascade enzyme catalytic reaction on a tyrosine phenol lyase product and a tyrosine ammonia lyase product to synthesize p-coumaric acid. According to the method, phenol, pyruvic acid and ammonium chloride are used as substrates, a tyrosine phenol lyase product and a tyrosine ammonia lyase product are used as catalysts, the tyrosine phenol lyase product can convert the substrates into L-tyrosine, and the tyrosine ammonia lyase product can directly use the L-tyrosine as the substrate to convert the L-tyrosine into p-coumaric acid. Therefore, the tyrosine phenol lyase product and the tyrosine ammonia lyase product can realize cascade enzyme catalytic reaction in the same reactor, phenol with lower cost can be adopted as a conversion substrate, separation and purification of an intermediate product L-tyrosine are avoided, and the whole process flow is simple and efficient.

The invention provides a preparation method of a metal organic coordination polymer mediated silver nanowire. The preparation method comprises the following steps: respectively preparing a supramolecular assembly agent, a high-molecular polymer protective agent, halide salt and a soluble silver salt solution; the supramolecular assembly agent is at least one of cinnamic acid, p-coumaric acid, caffeic acid, tannin and the like; halide salt and a supramolecular assembly agent are sequentially added into the soluble silver salt solution to form a silver ion-supramolecular assembly agent coordination polymer solution; adding a high-molecular polymer protective agent into the silver ion-supramolecular assembly agent coordination polymer solution, stirring, and reacting in a dark place at-20 to 80 DEG C for 0.1 to 10 hours; and after the reaction is completed, centrifuging, washing, drying in vacuum and re-dispersing to obtain the silver nanowire. According to the technical scheme, the prepared silver nanowire is superfine in diameter, the end has a curled shape, the interior contains defects, the reaction can be carried out in water, the productivity is high and is larger than 80 g / L, and the technology is simple and more environmentally friendly.

The present invention belongs to the field of fermentation engineering, and specifically relates to a yeast and its application in linseed oil fermentation and a linseed hydroponic oil product. The genetically recombinant yeast expresses phenylalanine deaminase, tyrosine deaminase, cinnamic acid hydroxylase, 4-coumaric acid CoA ligase, chalcone synthase, chalcone isomerase, flavonoid 3-hydroxylase and stilbene synthase. The linseed hydroponic oil obtained by fermenting genetically recombinant yeast and linseed oil in the present invention has enhanced stability and is not prone to mutation; and the linseed hydroponic oil has a higher total flavonoid content, better antioxidant activity and tyrosinase inhibition activity than linseed oil.

The present disclosure relates to microorganism(s) comprising modifications of genes in the shikimate pathway. The disclosure also relates to methods for modulating flux into shikimate pathway within a microorganism by selectively modulating gene expression of its components. Microorganism(s) of the disclosure are useful for the production of, e.g., 2-phenylethanol (2-PE), bakuchiol, p-coumaric acid, and its metabolites.

The invention relates to a combined bacterium system for efficiently synthesizing resveratrol and a method for synthesizing resveratrol, and belongs to the technical field of bioengineering and biosynthesis. The preparation method comprises the following steps: respectively connecting 4CL, STS and FCS with scaffold proteins, constructing recombinant expression vectors for expressing 4CL-SpyTag, STS-SpyCatcher and FCS-SpyTag, respectively introducing the recombinant expression vectors into escherichia coli, carrying out expression to obtain a compound enzyme connected with the scaffold proteins, and then carrying out self-assembly to obtain a multienzyme complex 4CL-SpyTag-STS-SpyCatcher and FCS-SpyTag-STS-SpyCatcher; the constructed recombinant engineering bacterium or enzyme complex is added into p-coumaric acid synthesized from agricultural wastes for further synthesis of resveratrol; the multi-enzyme complex constructed by the invention and the new method for synthesizing the resveratrol not only provide a new high-valued utilization approach for the lignin, but also realize efficient and high-yield synthesis of the resveratrol.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to yarrowia lipolytica engineering bacteria for producing isopentene aromatic compounds as well as a construction method and application of the yarrowia lipolytica engineering bacteria. Specifically, synthesis of two precursors of p-coumaric acid and dimethyl aryl pyrophosphate is engineered in parallel in two haploid yarrowia lipolytica strains. And then mating the receptor strain with the donor strain to construct the diploid capable of efficiently synthesizing the drpanin and the artepillin C. The invention further discloses a preparation method of the diploid of the drpanin and the artepillin C. By using a similar method, a strain capable of producing the 8-prenylnaringenin is also constructed. According to the present invention, the basis is provided for the sustainable production of drpanin, artepillin C and 8-prenylnaringenin, and the new way is provided for the rapid engineering of the yeast to produce other isopentenylated compounds.

The invention provides a tyrosine ammonia lyase mutant and application thereof in production of p-coumaric acid. The tyrosine ammonia lyase mutant is obtained by mutating wild type tyrosine ammonia lyase with an amino acid sequence as shown in SEQ ID No.1, and a mutation site is selected from at least one of Q108S, Y340Q and P375H. The catalytic activity center of wild type tyrosine ammonia lyase is analyzed, and site-directed mutagenesis is carried out on five catalytic activity center sites, so that the mutant shows different enzyme catalytic activity when the mutation sites are different, the mutation sites are the same but the mutation targets are different; q108S, Y340Q and P375H single mutations can significantly improve the enzyme catalytic activity of the tyrosine ammonia lyase mutant, and the tyrosine ammonia lyase mutant having any two mutation sites, especially having three mutation sites, also has better enzyme catalytic activity than the wild type, and has great application potential.

The invention discloses an apigenin derivative as well as a preparation method and application thereof. The apigenin derivative has the following molecular structure expression, and the preparation method comprises the following steps: 1) in the presence of an optional catalyst, reacting p-coumaric acid with ethyl sulfonyl chloride to generate an intermediate 1; and 2) mixing the purified intermediate 1 with oxalyl chloride for reaction, carrying out acylating chlorination, adding apigenin, and carrying out stirring reaction in the presence of an acid-binding agent to obtain the apigenin derivat.The apigenin derivative is novel in structure and has good biological activity for reducing the blood uric acid level in vivo.

The embodiment of the invention provides a tyrosine ammonia lyase mutant and application thereof in synthesis of p-coumaric acid. According to the embodiment of the invention, an amino acid sequence as shown in SEQ ID NO.2 is taken as a template, a key active site of the template is subjected to mutation transformation, a mutant library is constructed, and a series of mutants with remarkably improved catalytic activity and / or conversion rate are obtained through screening; the tyrosine ammonia lyase mutant provided by the embodiment of the invention can selectively catalyze L-tyrosine to generate p-coumaric acid, has excellent yield and / or higher yield, and has outstanding industrial application prospects in efficient and green synthesis of p-coumaric acid.

The invention discloses a determination method of an Ajowan fruit fingerprint spectrum and the fingerprint spectrum thereof, and relates to the technical field of fingerprint spectrums of medicinal materials. The method comprises the following steps: taking rutin, caffeic acid, rosmarinic acid and p-coumaric acid as reference substances, dissolving the reference substances in a solvent, and fixing the volume to obtain a reference substance solution; the method comprises the following steps: taking different batches of ajowan fruit medicinal materials as test products, adding a solvent into the test products, extracting, filtering, and taking subsequent filtrate to obtain a test product solution; respectively injecting the reference substance solution and the test solution into a liquid chromatograph by utilizing a high performance liquid chromatography, recording a chromatogram, and constructing the fingerprint spectrum of the Ajowan fruit according to the chromatogram; and determining the component content of the test solution through an external standard method according to the chromatogram. According to the method, the quality of the ajowan fruit medicinal material is controlled by adopting a method of combining chemical component content determination and a fingerprint spectrum, so that the effective and comprehensive control on the ajowan fruit medicinal material is realized, and a basis is provided for the quality control standard of the ajowan fruit medicinal material.

This invention relates to the field of traditional Chinese medicine detection technology, specifically providing characteristic chromatograms of germinated rice or roasted germinated rice and their preparations, and their construction methods. Using octadecylsilane-bonded silica gel as the packing material, the mobile phase includes methanol and formic acid aqueous solution, gradient elution, and a specific elution program obtained through extensive experimental screening, yielding more than 13 common characteristic peaks. Effective separation of characteristic peaks including uridine, adenosine, guanosine, 5-hydroxymethylfurfural, 4-coumaric acid, and ferulic acid is achieved, with good peak shape, stable baseline, complete separation of the measured components, and short detection time. This provides a basis for the quality detection and control of germinated rice or roasted germinated rice and their preparations, fully reflecting the integrity and characteristics of germinated rice or roasted germinated rice and their preparations. The method is simple to operate and applicable to the quality control of different types of germinated rice or roasted germinated rice preparations.

The application discloses a kind of compound Xianghua granules fingerprint's construction method, comprising the following steps: preparation of reference solution: p-coumaric acid, ferulic acid standard in methanol is dissolved;Preparation of test solution: different batches of compound Xianghua granules are dissolved in ultrapure water respectively, and post-processing is carried out;Respectively inject high performance liquid chromatography and detect, obtain the fingerprint of reference solution and the fingerprint of each batch sample;Using traditional Chinese medicine fingerprint similarity evaluation system analyzes the fingerprint of each batch sample, generates the control fingerprint of compound Xianghua granules.The method provided by the application can better reflect the multiple index component information of compound Xianghua granules.The fingerprint characteristic peak baseline of the application is smooth, and the sensitivity and separation degree are better, while the operation is quick and reliable, the cost is lower, can effectively, stably and quickly comprehensively monitor product quality, ensure the stability and quality controllability of product.

The invention relates to low-sugar gum soft sweets and a preparation method thereof. The soft sweets comprise the following raw materials in parts by weight: 18-35% of a pectin compound, 40-50% of a sweetening agent, 3-5% of a plasticizer, 0.3-0.8% of malic acid, 0.2-0.5% of sodium citrate and the balance of deionized water. Wherein the pectin compound comprises the following raw materials in percentage by weight: 10-20% of a cation matrix, 15-25% of an anion matrix, 5-10% of a neutral matrix and the balance of deionized water; the cation matrix comprises chitosan hydrochloride and calcium salt, the anion matrix comprises the following raw materials: low-methoxyl pectin, high-methoxyl pectin, ellagic acid and cumaric acid, and the neutral matrix is locust bean gum. The cation matrix, the anion matrix and the neutral matrix of the pectin compound cooperate to make up the defects of weak structure, poor water retention, easy aging and the like caused by sucrose deficiency in a low-sugar environment, so that the soft sweets have both elasticity and toughness.

The invention discloses a Rogowski eutrophy alkali-producing engineering strain for producing p-coumaric acid as well as a construction method and application of the Rogowski eutrophy alkali-producing engineering strain. The preparation method comprises the following steps: by taking a chemoautotrophic microorganism Ropriavidus alcaligenes H16 as an original strain, firstly, by knocking out a synthetic route of a strain poly-3-hydroxybutyrate (PHB), introducing a tyrosine ammonia lyase gene from Rhodotorula glutinis to construct a basic engineering strain capable of producing p-coumaric acid, and then, carrying out fermentation on the basic engineering strain to obtain the p-coumaric acid. Then, endogenous 3-deoxy-7-phosphoheptanone acid synthase gene aroG1 and chorismate synthase gene aroC are overexpressed, and the total copy is increased, so that the p-coumaric acid synthetic pathway flux of the engineering strain is enhanced; and introducing NAD (P) transhydrogenase gene pntAB from Escherichia coli to improve the supply of cofactors, so as to finally obtain the eutrophy alcaligenes Rosei engineering strain capable of synthesizing p-coumaric acid by using CO2.

The application provides a pharmaceutical composition for functional dyspepsia and a preparation method and application thereof, and belongs to the technical field of medicines, and the pharmaceutical composition comprises the following components: Aucklandia, Atractylodes, Mentha, Agastache, Citrus aurantium, Uncaria, Tribulus terrestris, Guangdong Shenqu, Chrysanthemum, Poria cocos, Radix Trichosanthis, Coix seed, Magnolol, Imperatorin, Isoimperatorin, Oxypeucedanin, Ferulic acid, Vanillic acid, p-Coumaric acid and Puerarin. The pharmaceutical composition comprises traditional Chinese medicine components and compounds, the components interact with each other, the effect of the pharmaceutical composition on improving functional dyspepsia can be improved, and meanwhile, the pharmaceutical composition has excellent stability.

This invention relates to the fields of synthetic biology and biochemical engineering, and provides an engineered *Yersinia lipolytica* strain that enhances coumaric acid accumulation, its construction method, and its applications. The engineered *Yersinia lipolytica* strain of this invention uses *Yersinia lipolytica* as the substrate strain, expresses the tyrosine ammonia-lyase encoding gene RgTAL derived from *Rhodotorula glutinis*, and completely knocks out the aminotransferase encoding gene containing the nucleotide sequence shown in SEQ ID NO 1 (database number YALI0A08734g). RgTAL converts endogenous L-tyrosine to coumaric acid. This invention reduces dependence on complex metabolic regulation and increases the coumaric acid accumulation level in *Yersinia lipolytica*.