168 results about "Coumaric acid" patented technology

The invention relates to a method for simultaneously preparing trans-ferulic acid, p-coumaric acid and pentosan, which comprises the following steps: processing a cellulose material with a low-concentration alkali to release coumaric acid, and hydrolyzing with a high-concentration alkali to release ferulic acid and pentosan; ultrafiltering the alkaline hydrolytic solution, precipitating the concentrated solution with ethanol to obtain pentosan; and adsorbing coumaric acid and ferulic acid in the filtrate with cation exchange resin, eluting with an ethanol-acid-water mixed eluent, removing ethanol from the eluent, crystallizing, centralizing or filtering the eluent at a low temperature or a normal temperature to obtain ferulic acid or coumaric acid crystals, and recrystallizing to obtain the high-purity product. The alkaline hydrolytic solution can be recovered and used for hydrolyzing the material, thereby obviating the production of a great amount of wastewater due to the alkaline hydrolysis. The method can produce ferulic acid and coumaric acid widely used in the industries of food, medicine and cosmetics, and pentosan used for producing xylooligosaccharide as food gum by utilizing solid wastes produced in agricultural and food processing, thereby achieving significant economic and social meanings.

The invention relates to cloning of a mulberry resveratrol synthase gene and construction of a plant expression vector. The gene has a nucleotide sequence shown by SEQ ID NO.7. Total RNA is extracted from fresh mulberry fruits, and the mulberry resveratrol synthase gene (MaRS) is cloned by using 3'RACE technology to obtain a complete gene sequence of 1496bp. A plant expression vector pCAMBIA2300-MaRS-Kana is constructed. The plant expression vector pCAMBIA2300-MaRS-Kana is transferred into agrobacterium C58 cells by electroporation, the activity of the enzyme coded by the cloned MaRS gene is verified by using a tobacco plant expression system, the MaRS gene can catalyze the combination of trans coumaric acid COA and malonyl-COA in tobacco to form resveratrol, and move the metabolic direction to downstream to synthesize a resveratrol derivative, and the gene is proved to be capable of improving the resveratrol content of a plant, therefore the MaRS gene has a development value of being applied to health food and beauty products. In addition, the MaRS gene can improve the disease resistance of the plant, thereby having a value of being applied to plant stress-tolerance gene engineering.

The invention discloses a method for preparing p-coumarate by catalyzing lignin depolymerization through hierarchical pore molecular sieve loaded molybdenum oxide. The method comprises the following steps: by taking lignin as a raw material, adding a reaction medium and a hierarchical pore molecular sieve loaded molybdenum oxide catalyst, replacing with nitrogen, pressurizing to 0.1-1MPa, heatingto 120-160 DEG C, reacting for 2-10 hours while stirring, removing the catalyst after the reaction, and catalytically degrading the lignin into p-coumarate. By regulating and controlling the catalyststructure, the reaction medium and the reaction condition factors, high-yield and high-selectivity depolymerization of lignin is achieved, p-coumarate serving as a high-added-value chemical is obtained, and the purpose of high-valued utilization of lignin is achieved. The heterogeneous catalyst is used, the process is simple, reaction conditions are mild, lignin can be selectively converted to obtain the target product p-coumarate, and the yield and selectivity of p-coumarate can reach up to 11.78 wt.% and 85.24 wt.% respectively.

The invention discloses a novel p-coumaric acid sulfonate derivative, a preparation method and application thereof. The structural formula of the novel p-coumaric acid sulfonate derivative is shown asformula (I) in the specification, wherein R is selected from the following groups shown as the specification. The compound provided by the invention has potential pharmaceutical activity, and the preparation raw material p-coumaric acid can be obtained by natural extraction method, thus having a positive effect on resource utilization of spartina alterniflora.

The invention discloses a separation method of effective components of a sharpleaf galangal fruit aqueous extract, belonging to the field of pharmaceutical analysis. The separation method comprises the following steps: (A) fetching dried sharpleaf galangal fruit, grinding, carrying out reflux extraction with water, combining filtrate, carrying out reduced pressure distillation to obtain extractum, dissolving the extractum into distilled water, respectively extracting by virtue of petroleum ether, ethyl acetate and normal butanol, and recycling solvents, so as to obtain ethyl acetate extract dry powder; (B) dissolving the dry powder obtain in the step (A), filtering, and processing by virtue of a sephadex LH20 gel column, so as to obtain six components from F1 to F6; and (C) carrying out HPLC separation and purification on the components from F2 to F6, so as to obtain compounds including protocatechualdehyde, protocatechuic acid, p-coumaric acid, (-)-epicatechin and (+)-catechinic acid. According to the separation method, the effective active components are separated and extracted from the sharpleaf galangal fruit, the structures of the effective components are further defined, the basis is provided for later medication, and required components can be extracted according to different requirements.

The invention relates to application of a chitosan derivative in bacterial wilt prevention and treatment. The structure of the chitosan derivative is shown in the formula (I), wherein R represents H or caffeic acid, or ferulic acid, or cinnamic acid, or p-coumaric acid or chlorogenic acid with a decarboxylated hydroxyl group. The medial lethal concentration of the chitosan derivative used for restraining bacterial wilt pathogenic bacteria is provided, the low-concentration chitosan derivative can be used for effectively restraining bacterial wilt bacteria, an effective method for preventing and treating bacterial wilt is provided, and the environment is not polluted.

The invention discloses a medicinal composition for treating a turtle cavity disease. The medicinal composition for treating the turtle cavity disease comprises the following Chinese herbs in parts by weight: 15-30 parts of hedyotis diffusa, 20-30 parts of groundsel, 5-15 parts of gallnut, 10-25 parts of golden cypress, 2-10 parts of wild chrysanthemum flower and 5-15 parts of onion. The invention further discloses a preparation of the medicinal composition. The hedyotis diffusa and the groundsel have a very strong anti-virus effect, so that the hedyotis diffusa and the groundsel as effective components can prevent and treat the turtle cavity disease better, with the cure rate as high as 99%. In the preparation method, the hedyotis diffusa is extracted with a supercritical carbon dioxide extraction method, so that effective components of the hedyotis diffusa, namely hentriacontane, stigmasterol, ursolic acid, oleanolic acid, beta-sitosterol, beta-sitosterol-D-glucoside and p-coumaric acid, have relatively high contents. The preparation method is simple and feasible and is applicable to large-scale production.

The invention relates to a method for extracting plant extract for preventing and treating diabetes, wherein linseed hulls are subjected to crushing and sieving, extracted by ethanol, subjected to alkaline hydrolysis, neutralization, concentration and spray drying to obtain the extract. The plant extract comprises the following effective constituents for treating or preventing diabetes: flax lignan, ferulic acid, coumaric acid and dietary fiber. The contents of various constituents are as follows respectively: 15 to 45 weight percent of the flax lignan, 1.2 to 6.0 weight percent of the ferulic acid, 1.0 to 4.0 weight percent of the coumaric acid and 40 to 70 weight percent of the dietary fiber. The method for extracting the plant extract for preventing and treating diabetes takes the naturally grown linseed hulls or naturally grown linseed cakes as a raw material of the extract, has simple manufacturing technique, low production cost, low investment and no harmful solvent residue, and is suitable for industrialized production. Simultaneously the variety of the effective constituents of the plant extract is numerous; the content is high; the preparation safety is high; the quality is stable; the plant extract can be orally taken for a long time and has no side effect; and medicines for preventing and treating symptoms such as hyperglycemia and so on caused by diabetes have good hypoglycemic effect and no adverse reaction as proved by clinic trails.

The present invention relates to a cosmetic composition for skin whitening comprising a small molecule-peptide conjugate. More specifically, it concerns the cosmetic composition for skin whitening comprising Caffeic acid-LG-NH₂ or Coumaric acid-LG-NH₂ as an active ingredient, which inhibits tyrosinase activity and melanin production, thereby showing an excellent skin whitening effect, and having no side effects to the skin by using a peptide component.

The invention relates to effective parts of schisandra bee pollen and the application thereof in liver injury prevention. The effective parts are obtained through extracting the schisandra bee pollen using ethanol, and the active ingredients and the content are gallic acid 15.78+/-1.64 mg/kg, protocatechuic acid 54.78+/-5.09 mg/kg, vanillic acid 64.21+/-5.13 mg/kg, p-coumaric acid 57.32+/-5.25 mg/kg, quercetin 273.63+/-20.84 mg/kg, hesperidin 741.56+/-76.8 mg/kg, kaempferol 387.34+/-35.65 mg/kg, and galangin 487.35+/-47.24 mg/kg. The inventor has found that the antioxidant activity of the schisandra bee pollen is higher than that of all known commercial bee pollen, and that the schisandra bee pollen can be used for preparing medicine or health care food for preventing liver injury caused by carbon tetrachloride.

The invention discloses a paulownia 4-coumaric-acid: coenzyme A ligase, encoding genes and application thereof. Amino acid residue sequence of the 4-coumaric-acid: coenzyme A ligase is shown in SEQ ID No. of 1. The information can be used for constructing genes of the 4-coumaric-acid: coenzyme A ligase, or antisense genes and RNA disturbing mass of the genes, which can be used for improving plant materials and constructing new materials with higher or lower lignin content.

The invention provides alkali-soluble light-curing epoxy resin containing cinnamic acid or coumarin groups, a preparation method of the resin and a solder resist prepared by using the same, wherein the alkali-soluble light-curing epoxy resin containing the cinnamic acid or coumarin groups is obtained through the steps that epoxy resin undergoes a ring-opening reaction to introduce a cinnamic acidderivative or coumaric acid, and then the product undergoes an esterification reaction with an acid anhydride. Compared with the prior art, during the process of introducing unsaturated groups into the epoxy resin, the preparation method does not need addition of a polymerization inhibitor; and compared with the prior art, the solder resist prepared by using the alkali-soluble light-curing epoxy resin has a lower photoinitiator additive amount, thereby improving the performance and application effect of the solder resist.

The invention discloses a method for producing verbascoside by using hairy roots of rehmannia, and belongs to the technical field of bio-culture. The method comprises the following step: putting the hairy roots of the rehmannia into a salicylic acid-containing culture solution for inducing culture to obtain the verbascoside. The theory of the method is that the hairy roots, which are treated by salicylic acid, of the rehmannia can enhance the expression of key biosynthetic enzymes, such as phenylalnine ammonialyase (PAL), 4-coumaroyl-co-A ligase (4CL), cinnamate 4-hydroxylase (C4H), coumaric acid hydroxylase (C3H), copper amine oxidase (CuAO) and tyrosine carboxy-lyase (TyDC), for the verbascoside within a short time period (12 to 24 h) so as to complete gathering of the verbascoside and further increase the yield of the verbascoside. The method is easy and convenient to operate, short in culture period, convenient for industrial, large-scale and standardized implementation, and has a great significance for promotion of large-scale production of the verbascoside.

The invention discloses a method for biosynthesizing a high-added-value compound by using lignocellulose derivatives, which comprises the following steps: A, modifying Escherichia coli to obtain a biocatalyst; B, synthesizing a high-added-value compound by taking a lignocellulose derivative as an initial raw material through a biocatalyst, wherein the lignocellulose derivative comprises at least one of p-coumaric acid and ferulic acid, and the high-added-value compound comprises at least one of gastrodin, arbutin, salidroside and derivatives thereof such as hydroquinone, tyrosol, hydroxytyrosol and styracitol. According to the method, escherichia coli is modified through a genetic engineering means, three new enzymatic reaction ways are constructed, and various high-added-value compounds including gastrodin, arbutin, salidroside and the like are efficiently synthesized by utilizing lignocellulose derived aromatic compounds p-coumaric acid and ferulic acid, wherein the product yield reaches gram-level or above.

The invention relates to the technical field of biology and discloses a method for heterologous biosynthesis of tonquinol, caffeol and ferulenol. The metabolic pathway for production of tonquinol, caffeol and ferulenol is added into hosts. More precisely, tyrosine ammonia lyase, cinnamoyl-coA reductase, alcohol dehydrogenase and coumaric acid coA ligase are efficiently expressed in the host for producing tonquinol, 4-hydroxyphenylacetic acid3-monooxygenase is added to produce caffeol, coffee acyl coenzyme AO-methyl transferase or caffeic acid O-methyl transferase is added to produce ferulenol. Genes coded by the enzyme are guided into the host, and the production host of tonquinol, caffeol and ferulenol can be generated by means of glucose. The invention further discloses a method for co-culture of different hosts, and the caffeol and the ferulenol are produced more efficiently.

The invention relates to a method for Escherichia coli whole cell catalyzed production of phloretin. The synthesis method is characterized in that the production of the phloretin is completed by adopting phenylalanine as a raw material and an Escherichia coli engineering bacterium as a host bacterium through catalysis of multiple enzymes in the host bacterium; and the host bacterium at least can express phenylalnine ammonialyase, cinnamic acid-4-hydroxylase, 4-coumaric acid coenzyme A ligase, double-bond reductase and chalcone synthetase. The method has the following advantages: 1, the phenylalanine is used as the raw material, so the raw material cost of the method is low; 2, catalytic synthesis of biological enzymes in microorganisms is carried out based on microorganism modification, solarge-scale extraction, separation and purification processes are avoided, and the production cost and the environmental protection are easy to control; 3, the size and the quantity of production devices used in the method are less than those of other methods, so the method can be easily industrially converted; and 4, only the final step in the production of the method contains separation and purification processes, so the product purifying process is simple, and the product has better quality and higher content than the product obtained through general methods.

The invention provides a Kluyveromyces marxianus recombinant strain capable of being used for preparing feruloyl esterase AnfaeA. The recombinant strain is constructed by cloning an aspergillus nigerferuloyl esterase AnfaeA gene with an optimized sequence to an expression vector and converting a Kluyveromyces marxianus host strain, and the yield of the feruloyl esterase obtained by high-density fermentation of the recombinant strain reaches 120000 U/ml. The invention also provides a method for preparing ferulic acid by hydrolyzing corncob powder with the feruloyl esterase. The feruloyl esterase AnfaeA prepared from the recombinant strain and xylanase have a synergistic effect, so that the corncob powder can be efficiently hydrolyzed to obtain the ferulic acid. The feruloyl esterase provided by the invention can be used for preparing phenolic substances such as ferulic acid, p-coumaric acid and the like through biodegradation of agricultural byproducts such as corncobs, rice bran, cornbran and the like, so that the feruloyl esterase has a wide application value in the fields of foods, papermaking, feeds, medicines and the like. Meanwhile, the preparation method is simple in process, high in yield and very suitable for large-scale production.

The invention relates to the plant dormancy adjustment and control technical field, and discloses a dormancy agent for transplanting trees; each 1 L of the dormancy agent for transplanting the trees comprises the following components: 0.1-0.5 g of a polyamine biosynthetic inhibitor, 0.15-0.2 g of calcium chloride, 0.01-0.1 g of catechol, 0.01-0.05 g of coumaric acid, 0.005-0.01 g of salicylic acid, 0.008-0.05 g of abscisic acid, 6-8 ml of Tween 20, and the balance water. The invention also discloses a preparation method of the dormancy agent for transplanting the trees. The trees are subjected to dormancy induction before the trees are transplanted, seasonal restriction and technical barriers of tree full-year transplanting are overcome, and the transplanting survival rate of the trees is increased. The cost is low, the consumption is little, the trees restore quickly, the transplanting security coefficient is high, and the landscape effect is easy to form.

The invention relates to cytochrome P450 reductase as well as coding gene and application thereof. The invention discloses cytochrome P450 reductase 1 of lycoris aurea as an amaryllidaceae plant for the first time; the cytochrome P450 reductase 1 has favorable coenzyme specificity and can be used for assisting a cytochrome P450 enzyme in exerting catalytic activity to modify a synthetic product of a substrate of the cytochrome P450 enzyme in an oxidative manner. The invention also discloses a polynucleotide for coding the cytochrome P450 reductase, a carrier and a host cell for expressing the cytochrome P450 reductase and a method for producing p-coumaric acid.

A cell may include heterologous polynucleotides encoding a multienzyme complex involved in the metabolic pathway of phenylpropanoids and biosynthesis of a vanilloid or a hydroxybenzaldehyde precursor thereof, which multienzyme complex comprises enzymes for the biosynthesis of coumaric acid and a crotonase.

The invention relates to p-coumaric acid-3-hydroxylase as well as a coding gene and an application thereof. The p-coumaric acid-3-hydroxylase of lycoris plant lycoris aurea is disclosed for the first time, has good substrate specificity and region selectivity, and can catalyze hydroxylation of 3-position C of p-coumaric acid to produce coffeic acid. The invention further discloses polunecleotide coding the p-coumaric acid-3-hydroxylase, a vector and a host cell expressing the p-coumaric acid-3-hydroxylase as well as a coffeic acid production method.