148 results about "Coumaric acid" patented technology

The embodiment of the invention provides a tyrosine ammonia lyase mutant and application thereof in synthesis of p-coumaric acid. The mutant is subjected to any one of the following amino acid mutations on an amino acid sequence as shown in SEQ ID NO. 2: F497I, F497I + L248M, F497I + L248M + M4T, and L248M + F497I + M4T + V482L. According to the embodiment of the invention, an amino acid sequence as shown in SEQ ID NO. 2 is taken as a template, a key active site of the template is subjected to mutation transformation, a mutant library is constructed, and a series of mutants with remarkably improved catalytic activity and / or conversion rate are screened; the tyrosine ammonia lyase mutant provided by the embodiment of the invention can selectively catalyze L-tyrosine to generate p-coumaric acid, and has excellent yield and / or higher yield.

The embodiment of the invention provides a method for obtaining a tyrosine ammonialyase mutant based on high-throughput screening, the tyrosine ammonialyase mutant and application of the tyrosine ammonialyase mutant. The mutant is subjected to any one of the following amino acid mutations on an amino acid sequence as shown in SEQ ID NO.2: A245G + A247T, and D192V + A245G + A247T. According to the embodiment of the invention, an amino acid sequence as shown in SEQ ID NO.2 is taken as a template (basis), a key active site of the template is subjected to mutation transformation, a mutant library is constructed, and a series of mutants with remarkably improved catalytic activity and / or conversion rate are screened; the tyrosine ammonia lyase mutant provided by the embodiment of the invention can selectively catalyze L-tyrosine to generate p-coumaric acid, and has excellent yield and / or higher yield, which indicates that the mutant has more significant application prospects in catalysis of L-tyrosine to generate p-coumaric acid.

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The embodiment of the invention provides a recombinant pichia pastoris strain as well as a construction method and application thereof. The construction method comprises the following steps: by taking a pichia pastoris strain as a host strain, constructing a dual-synthesis path of phenylalanine ammonialyase and tyrosine ammonialyase in the host strain to obtain a first engineering strain; removing chorismic acid feedback inhibition of the first engineering strain based on metabolic engineering to obtain a second engineering strain; optimizing an aromatic amino acid synthesis route of the second engineering strain to obtain a third engineering strain; knocking out a branch metabolic pathway of the third engineering strain to obtain a fourth engineering strain; increasing precursor supply of the fourth engineering strain to obtain a recombinant pichia pastoris strain; according to the construction method provided by the embodiment of the invention, the synthesis path of p-coumaric acid can be systematically optimized, the recombinant pichia pastoris strain for efficiently producing p-coumaric acid by taking methanol as a carbon source is constructed, and compared with a host strain, the recombinant pichia pastoris strain has the advantage that the yield of p-coumaric acid is remarkably increased.

The invention discloses a p-coumaric acid biosensor and application thereof, and the p-coumaric acid biosensor is characterized in that a coding gene of a CarR mutant, a Plpp1.6 promoter, a coding gene of a green fluorescent protein and a Pcar promoter mutant for controlling the expression of the green fluorescent protein are located on an expression vector pTrc99a; the biosensor provided by the invention realizes real-time sensitive monitoring of the concentration of coumaric acid by a recombinant escherichia coli strain containing the coumaric acid biosensor, can be combined with a high-throughput mutagenesis and screening system, and is beneficial to rapid and efficient screening of high-yield engineering strains of coumaric acid; and a technical guarantee is provided for production of p-coumaric acid and derivatives by efficient fermentation of microorganisms. The p-coumaric acid biosensor based on transcriptional regulatory factors is constructed, and a new strategy is provided for high-throughput screening.

The present invention relates to the field of biotechnology, and particularly to the application of recombinant Saccharomyces cerevisiae in the production of homopterocarpin using p-coumaric acid. The present invention provides an engineered Saccharomyces cerevisiae capable of producing homopterocarpin using p-coumaric acid. Through strategies such as artificial pathway construction, pathway optimization, rational design of enzymes, and fermentation process optimization, the present invention obtains an engineered Saccharomyces cerevisiae capable of producing homopterocarpin using p-coumaric acid. Experiments show that its homopterocarpin yield is 1.005 mmol / l and the conversion rate is 65.8%. In large-scale production in a 5L fermenter, the strain of the present invention can utilize 15.2 mmol / l of p-coumaric acid to produce 3.2 mmol / l of homopterocarpin, and the conversion rate is 21.1%.

The invention provides a method for judging a processing end point of fried rice sprouts and a processing technology of the fried rice sprouts, and the method for judging the processing end point of the fried rice sprouts comprises the following steps: (1) mixing the fried rice sprouts with different frying time and an alcoholic solution, and carrying out ultrasonic extraction to obtain a series of test solution; (2) carrying out liquid chromatography detection on the series of test solutions, calculating peak area ratios of 4-coumaric acid to 5-hydroxymethylfurfural in the fried rice sprouts at different stir-frying times according to detection results, and taking a time period corresponding to a stable peak area ratio range between ratio mutation points as stir-frying time required for reaching a processing end point, and judging the processing end point of the fried rice sprouts. The method provided by the invention can objectively, accurately and clearly judge the processing end point of the fried rice sprouts, fills the blank of judgment of the processing end point of the fried rice sprouts, makes up for the situation of misjudgment caused by evaluation of the processing end point only by colors, realizes monitoring of the processing process of the fried rice sprouts, and can effectively guide the processing technology of the fried rice sprouts.

The application provides a recombinant Pichia pastoris strain and a construction method and application thereof. The construction method comprises the following steps: taking a Pichia pastoris strain as a host strain, constructing a double synthesis pathway of phenylalanine ammonia lyase and tyrosine ammonia lyase in the host strain to obtain a first engineering strain; relieving the branch acid feedback inhibition of the first engineering strain based on metabolic engineering to obtain a second engineering strain; optimizing the aromatic amino acid synthesis pathway of the second engineering strain to obtain a third engineering strain; knocking out the branch metabolic pathway of the third engineering strain to obtain a fourth engineering strain; increasing the precursor supply of the fourth engineering strain to obtain the recombinant Pichia pastoris strain. The construction method can systematically optimize the synthesis path of p-coumaric acid, and the recombinant Pichia pastoris strain for efficiently producing p-coumaric acid with methanol as a carbon source is constructed. Compared with the host strain, the yield of p-coumaric acid of the recombinant Pichia pastoris strain is significantly improved.

The invention discloses a method for producing p-coumaric acid through double-enzyme cascade catalysis, which comprises the following steps: taking phenol, pyruvic acid and ammonium chloride as substrates, and carrying out cascade enzyme catalytic reaction on a tyrosine phenol lyase product and a tyrosine ammonia lyase product to synthesize p-coumaric acid. According to the method, phenol, pyruvic acid and ammonium chloride are used as substrates, a tyrosine phenol lyase product and a tyrosine ammonia lyase product are used as catalysts, the tyrosine phenol lyase product can convert the substrates into L-tyrosine, and the tyrosine ammonia lyase product can directly use the L-tyrosine as the substrate to convert the L-tyrosine into p-coumaric acid. Therefore, the tyrosine phenol lyase product and the tyrosine ammonia lyase product can realize cascade enzyme catalytic reaction in the same reactor, phenol with lower cost can be adopted as a conversion substrate, separation and purification of an intermediate product L-tyrosine are avoided, and the whole process flow is simple and efficient.

The invention relates to a combined bacterium system for efficiently synthesizing resveratrol and a method for synthesizing resveratrol, and belongs to the technical field of bioengineering and biosynthesis. The preparation method comprises the following steps: respectively connecting 4CL, STS and FCS with scaffold proteins, constructing recombinant expression vectors for expressing 4CL-SpyTag, STS-SpyCatcher and FCS-SpyTag, respectively introducing the recombinant expression vectors into escherichia coli, carrying out expression to obtain a compound enzyme connected with the scaffold proteins, and then carrying out self-assembly to obtain a multienzyme complex 4CL-SpyTag-STS-SpyCatcher and FCS-SpyTag-STS-SpyCatcher; the constructed recombinant engineering bacterium or enzyme complex is added into p-coumaric acid synthesized from agricultural wastes for further synthesis of resveratrol; the multi-enzyme complex constructed by the invention and the new method for synthesizing the resveratrol not only provide a new high-valued utilization approach for the lignin, but also realize efficient and high-yield synthesis of the resveratrol.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to yarrowia lipolytica engineering bacteria for producing isopentene aromatic compounds as well as a construction method and application of the yarrowia lipolytica engineering bacteria. Specifically, synthesis of two precursors of p-coumaric acid and dimethyl aryl pyrophosphate is engineered in parallel in two haploid yarrowia lipolytica strains. And then mating the receptor strain with the donor strain to construct the diploid capable of efficiently synthesizing the drpanin and the artepillin C. The invention further discloses a preparation method of the diploid of the drpanin and the artepillin C. By using a similar method, a strain capable of producing the 8-prenylnaringenin is also constructed. According to the present invention, the basis is provided for the sustainable production of drpanin, artepillin C and 8-prenylnaringenin, and the new way is provided for the rapid engineering of the yeast to produce other isopentenylated compounds.

The invention provides a tyrosine ammonia lyase mutant and application thereof in production of p-coumaric acid. The tyrosine ammonia lyase mutant is obtained by mutating wild type tyrosine ammonia lyase with an amino acid sequence as shown in SEQ ID No.1, and a mutation site is selected from at least one of Q108S, Y340Q and P375H. The catalytic activity center of wild type tyrosine ammonia lyase is analyzed, and site-directed mutagenesis is carried out on five catalytic activity center sites, so that the mutant shows different enzyme catalytic activity when the mutation sites are different, the mutation sites are the same but the mutation targets are different; q108S, Y340Q and P375H single mutations can significantly improve the enzyme catalytic activity of the tyrosine ammonia lyase mutant, and the tyrosine ammonia lyase mutant having any two mutation sites, especially having three mutation sites, also has better enzyme catalytic activity than the wild type, and has great application potential.

The invention discloses an apigenin derivative as well as a preparation method and application thereof. The apigenin derivative has the following molecular structure expression, and the preparation method comprises the following steps: 1) in the presence of an optional catalyst, reacting p-coumaric acid with ethyl sulfonyl chloride to generate an intermediate 1; and 2) mixing the purified intermediate 1 with oxalyl chloride for reaction, carrying out acylating chlorination, adding apigenin, and carrying out stirring reaction in the presence of an acid-binding agent to obtain the apigenin derivat.The apigenin derivative is novel in structure and has good biological activity for reducing the blood uric acid level in vivo.

The embodiment of the invention provides a tyrosine ammonia lyase mutant and application thereof in synthesis of p-coumaric acid. According to the embodiment of the invention, an amino acid sequence as shown in SEQ ID NO.2 is taken as a template, a key active site of the template is subjected to mutation transformation, a mutant library is constructed, and a series of mutants with remarkably improved catalytic activity and / or conversion rate are obtained through screening; the tyrosine ammonia lyase mutant provided by the embodiment of the invention can selectively catalyze L-tyrosine to generate p-coumaric acid, has excellent yield and / or higher yield, and has outstanding industrial application prospects in efficient and green synthesis of p-coumaric acid.

The invention discloses a determination method of an Ajowan fruit fingerprint spectrum and the fingerprint spectrum thereof, and relates to the technical field of fingerprint spectrums of medicinal materials. The method comprises the following steps: taking rutin, caffeic acid, rosmarinic acid and p-coumaric acid as reference substances, dissolving the reference substances in a solvent, and fixing the volume to obtain a reference substance solution; the method comprises the following steps: taking different batches of ajowan fruit medicinal materials as test products, adding a solvent into the test products, extracting, filtering, and taking subsequent filtrate to obtain a test product solution; respectively injecting the reference substance solution and the test solution into a liquid chromatograph by utilizing a high performance liquid chromatography, recording a chromatogram, and constructing the fingerprint spectrum of the Ajowan fruit according to the chromatogram; and determining the component content of the test solution through an external standard method according to the chromatogram. According to the method, the quality of the ajowan fruit medicinal material is controlled by adopting a method of combining chemical component content determination and a fingerprint spectrum, so that the effective and comprehensive control on the ajowan fruit medicinal material is realized, and a basis is provided for the quality control standard of the ajowan fruit medicinal material.

This invention relates to the field of traditional Chinese medicine detection technology, specifically providing characteristic chromatograms of germinated rice or roasted germinated rice and their preparations, and their construction methods. Using octadecylsilane-bonded silica gel as the packing material, the mobile phase includes methanol and formic acid aqueous solution, gradient elution, and a specific elution program obtained through extensive experimental screening, yielding more than 13 common characteristic peaks. Effective separation of characteristic peaks including uridine, adenosine, guanosine, 5-hydroxymethylfurfural, 4-coumaric acid, and ferulic acid is achieved, with good peak shape, stable baseline, complete separation of the measured components, and short detection time. This provides a basis for the quality detection and control of germinated rice or roasted germinated rice and their preparations, fully reflecting the integrity and characteristics of germinated rice or roasted germinated rice and their preparations. The method is simple to operate and applicable to the quality control of different types of germinated rice or roasted germinated rice preparations.

The application discloses a kind of compound Xianghua granules fingerprint's construction method, comprising the following steps: preparation of reference solution: p-coumaric acid, ferulic acid standard in methanol is dissolved;Preparation of test solution: different batches of compound Xianghua granules are dissolved in ultrapure water respectively, and post-processing is carried out;Respectively inject high performance liquid chromatography and detect, obtain the fingerprint of reference solution and the fingerprint of each batch sample;Using traditional Chinese medicine fingerprint similarity evaluation system analyzes the fingerprint of each batch sample, generates the control fingerprint of compound Xianghua granules.The method provided by the application can better reflect the multiple index component information of compound Xianghua granules.The fingerprint characteristic peak baseline of the application is smooth, and the sensitivity and separation degree are better, while the operation is quick and reliable, the cost is lower, can effectively, stably and quickly comprehensively monitor product quality, ensure the stability and quality controllability of product.

The invention relates to low-sugar gum soft sweets and a preparation method thereof. The soft sweets comprise the following raw materials in parts by weight: 18-35% of a pectin compound, 40-50% of a sweetening agent, 3-5% of a plasticizer, 0.3-0.8% of malic acid, 0.2-0.5% of sodium citrate and the balance of deionized water. Wherein the pectin compound comprises the following raw materials in percentage by weight: 10-20% of a cation matrix, 15-25% of an anion matrix, 5-10% of a neutral matrix and the balance of deionized water; the cation matrix comprises chitosan hydrochloride and calcium salt, the anion matrix comprises the following raw materials: low-methoxyl pectin, high-methoxyl pectin, ellagic acid and cumaric acid, and the neutral matrix is locust bean gum. The cation matrix, the anion matrix and the neutral matrix of the pectin compound cooperate to make up the defects of weak structure, poor water retention, easy aging and the like caused by sucrose deficiency in a low-sugar environment, so that the soft sweets have both elasticity and toughness.

The invention discloses a Rogowski eutrophy alkali-producing engineering strain for producing p-coumaric acid as well as a construction method and application of the Rogowski eutrophy alkali-producing engineering strain. The preparation method comprises the following steps: by taking a chemoautotrophic microorganism Ropriavidus alcaligenes H16 as an original strain, firstly, by knocking out a synthetic route of a strain poly-3-hydroxybutyrate (PHB), introducing a tyrosine ammonia lyase gene from Rhodotorula glutinis to construct a basic engineering strain capable of producing p-coumaric acid, and then, carrying out fermentation on the basic engineering strain to obtain the p-coumaric acid. Then, endogenous 3-deoxy-7-phosphoheptanone acid synthase gene aroG1 and chorismate synthase gene aroC are overexpressed, and the total copy is increased, so that the p-coumaric acid synthetic pathway flux of the engineering strain is enhanced; and introducing NAD (P) transhydrogenase gene pntAB from Escherichia coli to improve the supply of cofactors, so as to finally obtain the eutrophy alcaligenes Rosei engineering strain capable of synthesizing p-coumaric acid by using CO2.

The application provides a pharmaceutical composition for functional dyspepsia and a preparation method and application thereof, and belongs to the technical field of medicines, and the pharmaceutical composition comprises the following components: Aucklandia, Atractylodes, Mentha, Agastache, Citrus aurantium, Uncaria, Tribulus terrestris, Guangdong Shenqu, Chrysanthemum, Poria cocos, Radix Trichosanthis, Coix seed, Magnolol, Imperatorin, Isoimperatorin, Oxypeucedanin, Ferulic acid, Vanillic acid, p-Coumaric acid and Puerarin. The pharmaceutical composition comprises traditional Chinese medicine components and compounds, the components interact with each other, the effect of the pharmaceutical composition on improving functional dyspepsia can be improved, and meanwhile, the pharmaceutical composition has excellent stability.

This invention relates to the fields of synthetic biology and biochemical engineering, and provides an engineered *Yersinia lipolytica* strain that enhances coumaric acid accumulation, its construction method, and its applications. The engineered *Yersinia lipolytica* strain of this invention uses *Yersinia lipolytica* as the substrate strain, expresses the tyrosine ammonia-lyase encoding gene RgTAL derived from *Rhodotorula glutinis*, and completely knocks out the aminotransferase encoding gene containing the nucleotide sequence shown in SEQ ID NO 1 (database number YALI0A08734g). RgTAL converts endogenous L-tyrosine to coumaric acid. This invention reduces dependence on complex metabolic regulation and increases the coumaric acid accumulation level in *Yersinia lipolytica*.

A MATZHU is prepared by using leaves of Gramineae (Graminae) and Bambusoideae plant as raw materials, and has a stable emerald color, and has an average powder particle size of 800 to 10,000 meshes, and has a total amount of dietary fiber of ≥60%, a content of lignin of ≥20% and a content of minerals of ≥7%, and comprises at least three or more bamboo leaf characteristic components, such as orientin, isoorientin, vitexin, isovitexin, adenosine, δ-hydroxylysine and p-coumaric acid. The method for the MATZHU preparation comprises, in turn, performing blanching and color protection, drying and superfine grinding the raw materials. By utilizing the thermal stability and the light stability of the MATZHU, the MATZHU may be used as a raw food material, a functional ingredient, or a dietary supplement.

According to the genetically engineered bacterium for high yield of p-coumaric acid and the application of the genetically engineered bacterium, tyrosine ammonia lyase from different sources and expression vectors with different copy numbers are combined to optimize metabolic pathways, so that the yield of p-coumaric acid is increased. The method specifically comprises the following steps: constructing recombinant expression vectors by using tyrosine ammonia lyase FjTAL from flavobacterium johnsonii and tyrosine ammonia lyase FsTAL from flavobacterium cissii and expression vectors pACYCDuet-1, pCDFDuet-1, pETCDuet-1 and pRSFDuet-1 with different copy numbers respectively by using a homologous recombination technology, transferring the recombinant expression vectors into escherichia coli BL21 (DE3) to obtain engineering strains pCA1-pCA8, and carrying out fermentation on the engineering strains pCA1-pCA8 to obtain the tyrosine ammonia lyase FjTAL from flavobacterium johnsonii and the tyrosine ammonia lyase FsTAL from flavobacterium cissii. Glucose is used as a substrate for fermentation and screening to obtain the high-yield engineering bacterium pCA8, and the yield of p-coumaric acid of the engineering bacterium pCA8 reaches 251.61 mg / L. By optimizing key enzyme expression, tyrosine is efficiently promoted to be converted into p-coumaric acid, and the method has the advantages of being low in cost, high in efficiency, environmentally friendly and the like, is suitable for industrial production and can be widely applied to the fields of biomedicine, food, cosmetics and the like.

The invention provides a method for detecting the content of 4-coumaric acid and ferulic acid in polygonum-folium isatidis formula granules. The method comprises the following steps: acquiring a content detection map of a to-be-detected substance by adopting a high performance liquid chromatography; chromatographic conditions of the high performance liquid chromatography are as follows: octadecylsilane chemically bonded silica is used as a chromatographic column of a filler; the detection wavelength of 1, 4-cumaric acid is 290 to 330nm, and the detection wavelength of ferulic acid is 305 to 340nm; and acetonitrile and a 0.5%-1.5% glacial acetic acid solution in a ratio of (5-15): (85-95) are used as mobile phases. According to the method disclosed by the invention, organic acid components, namely 4-coumaric acid and ferulic acid, in an aqueous extract of the folium polygoni multiflori are taken as reference substances, and the high-performance liquid phase content determination and detection method for the coumaric acid and the ferulic acid in the folium polygoni multiflori formula granules is established, so that the contents of the 4-coumaric acid and the ferulic acid in the folium polygoni multiflori can be accurately and synchronously detected; the quality of a product can be better controlled on the whole, and effective guarantee is provided for production and clinical application of drugs.

The present application belongs to the field of livestock and poultry breeding and feed processing technology, and particularly relates to a feed additive, a nutritional supplement, a feed and application thereof. The present application provides application of p-coumaric acid in improving carcass and meat quality, improving feed conversion rate, reducing fat deposition of livestock and poultry and improving fat metabolism of livestock and poultry. The results of the examples show that p-coumaric acid can effectively improve the feed conversion efficiency of growing pigs in the growth stage, which provides a reference for reducing feed waste in production and realizing cost reduction and benefit increase of animal husbandry; under the premise of not reducing or even improving the growth performance and slaughter rate of growing and fattening pigs, the p-coumaric acid can reduce the fat thickness, improve the pig carcass rating, improve the meat quality, and is beneficial to realize efficient production of high-quality pork and improve the market competitiveness of pork in China; the p-coumaric acid can also reduce the blood lipids of chickens, reduce fatty liver and reduce subcutaneous fat deposition, maintain normal metabolism of chickens and improve the health of chickens.

The invention belongs to the technical field of biology, and discloses a saccharomyces cerevisiae engineering strain for producing naringenin, a construction method and application, the saccharomyces cerevisiae engineering strain contains tyrosine ammonia lyase gene TAL, 4-cumaric acid: coenzyme A ligase gene 4CL, chalcone synthase gene CHS, chalcone isomerase gene CHI, oligopeptide gene RIADn-RGGm and oligopeptide gene RIDD; the ARO10 gene, the FDC1 gene, the PAD1 gene and the PDC5 gene are knocked out. The engineering strain disclosed by the invention can be used for synthesizing naringenin by taking L-tyrosine as a substrate, and the engineering strain is stable in hereditary character and excellent in naringenin yield which can reach 27.45 mg / L; and the fermentation production of the product can be carried out through relatively simple method conditions, so that the method has a very wide application prospect.

The invention provides a herbicide for preventing and treating cyperus rotundus in cooperation with glyphosate. The herbicide is prepared from bacillus velezensis fermentation liquor, phenolic acid and glyphosate. The phenolic acid comprises any one or a combination of more of p-coumaric acid, cinnamic acid, p-hydroxybenzoic acid and caffeic acid. The bacillus velezensis fermentation liquor and phenolic acid components are introduced and form a synergistic effect with the glyphosate, so that the dosage of a single dosage of the glyphosate is reduced, the control effect is guaranteed, meanwhile, the potential pressure of chemical pesticide input on the ecological environment is reduced, and the environmental compatibility of a weeding system is improved.

The application provides a tyrosine deaminase mutant and application thereof in production of p-coumaric acid. The tyrosine deaminase mutant is obtained by mutating a wild-type tyrosine deaminase with an amino acid sequence shown in SEQ ID No. 1, and the mutation site is selected from at least one of Q108S, Y340Q and P375H. The application analyzes a catalytic active center of the wild-type tyrosine deaminase, and finds that different mutation sites, same mutation sites but different mutation targets can make the mutant show completely different enzyme catalytic activities, and the single mutation of Q108S, Y340Q and P375H can significantly improve the enzyme catalytic activity of the tyrosine deaminase mutant, and the tyrosine deaminase mutant with any two mutation sites, especially with three mutation sites also has excellent enzyme catalytic activity than the wild type, and has great application potential.

The invention relates to the technical field of paint, and discloses environment-friendly paint and a preparation method thereof.The environment-friendly paint is prepared from phenyl acrylate, styrene, lauryl methacrylate, tert-butyl acrylate, anti-fouling and anti-corrosion cellulose, coumaric acid modified polyether, absolute ethyl alcohol, an initiator, a simethicone defoaming agent and a polyurethane flatting agent; the anti-fouling and anti-corrosion cellulose and coumaric acid modified polyether are prepared to participate in the preparation process of the paint matrix, so that the prepared environment-friendly paint has excellent anti-fouling and anti-corrosion capability, is high in toughness and not easy to damage, also has an excellent anti-oxidation effect, can meet the use requirements in various environments, and has a wide application prospect. And even if used for a long time, the device does not need to be replaced frequently, thereby saving cost and having long service life.