533 results about "Heterologous expression" patented technology

This application relates to the field of biosynthesis technology, and specifically discloses a pyridone compound, its biosynthesis method and application. Based on synthetic biology-related technologies, this application modifies non-ribosomal peptide synthetase, heterologously expresses non-ribosomal peptide synthetase mutants in a variety of hosts, and for the first time discovers and obtains a pyridone compound, and determines its chemical formula as C5H4N2O3, named 5-amino-pyridine-2,3,6-trione or 5-hydroxy-3-imino-pyridine-2,6-dione. This novel pyridone compound can be used as a raw material or intermediate in the development and preparation of novel antibiotics, drugs and dyes.

The invention discloses a 3 '-phosphoadenosine-5'-phosphoryl sulfate synthetase mutant and an application of the 3 '-phosphoadenosine-5'-phosphoryl sulfate synthetase mutant. Compared with a wild enzyme amino acid sequence as shown in SEQ ID NO.1, the amino acid sequence of the 3 '-adenosine-5'-phosphoryl sulfate synthetase mutant has one or two mutations in the 101 site, the 207 site and the 560 site; preferably, phenylalanine at the 101 site is mutated into tryptophan; cysteine at the 207th site is mutated into glycine; phenylalanine at the 560th site is mutated into tryptophan. The invention also provides a synthesis method and application of the 3 '-adenosine-5'-phosphoryl sulfuric acid. The APS intermediate product inhibition effect of hPAPSS1 is relieved through a point mutation technology, the substrate affinity of hPAPSS1 to ATP is improved, and a mutant enzyme with a high PAPS conversion rate is obtained; and the bifunctional enzyme hPAPSS1 is synthesized by heterologous expression of PAPS in escherichia coli, so that the acquisition of the enzyme is simplified, and the synthesis efficiency of PAPS is improved.

The invention relates to an L-valine production strain as well as a construction method and application thereof, the strain is obtained by transformation through a metabolic synthesis path of L-valine, and the construction method comprises the following steps: by taking Cornebacterium glutamicum ATCC 13032 (C.glutamicum) as an original strain, carrying out editing transformation such as gene knockout, gene overexpression, gene heterologous expression, gene double-copy expression and the like on a genome of the original strain by utilizing a metabolic engineering means. The L-valine production strain disclosed by the invention can be used for synthesizing L-valine from the beginning by taking glucose as a carbon source, has the advantages of high production rate, short fermentation period, high strain stability and low cost, and lays a foundation for realizing large-scale production of L-valine. According to the fermentation tank fermentation method, the L-valine is produced in a mechanical stirring type fermentation tank by using the L-valine production strain Va1-09, 86.4 g / L of L-valine is produced by fermenting for 36 hours, and the method has a good industrial prospect.

The invention relates to the technical field of biology, in particular to glycopeptide antibiotic and application thereof. According to the invention, a biosynthetic gene cluster for synthesizing a brand new glycopeptide antibiotic skeleton is excavated, and brand new glycopeptide antibiotics Varsomycin A, B and C with novel chemical structures are obtained through heterologous expression; the traditional Chinese medicine composition has a remarkable antibacterial effect on clinically drug-resistant strains. Important fermentation strains and precursor compound molecules are provided for research and development of new drugs of glycopeptide antibiotics, and important application prospects and economic values are achieved.

The invention discloses a fusion protein which comprises a first structural domain with an EgtD catalytic function, a second structural domain with an Egt1 catalytic function and a third structural domain with an EgtE catalytic function, and the amino acid sequence of the first structural domain with the EgtD catalytic function is shown as SEQ ID NO.1; the amino acid sequence of the second structural domain with the Egt1 catalytic function is as shown in SEQ ID NO. 2; and the amino acid sequence of the third structural domain with the EgtE catalytic function is as shown in SEQ ID NO. 3. The fusion protein is subjected to heterologous expression in engineering bacteria, so that the yield of ergothioneine is remarkably increased.

The invention discloses a pichia pastoris engineering bacterium for synthesizing bakuchiol from de novo by converting methanol as well as construction and application of the pichia pastoris engineering bacterium. A heterologous p-coumaric acid synthesis pathway is introduced to neutral sites of different chromosomes of pichia pastoris, a high-yield p-coumaric acid strain is obtained through gene knockout, overexpression or heterologous expression of tyrosine and phenylalanine synthesis pathway key genes of a shikimic acid pathway, a heterologous bakuchiol biosynthesis pathway is introduced on the basis, and a high-yield p-coumaric acid strain is obtained. After overexpression of bakuchiol synthetase, endogenous overexpression or heterologous expression of MVA pathway key genes and exogenous acetyl coenzyme A supply pathway genes, methanol concentration optimization and bacterial strain His4 gene supplementation, the yield of bakuchiol is effectively increased to 91.2 mg / L and is increased by 59.8 times compared with that of an initial bacterial strain, and the yield of bakuchiol in a 15L fermentation tank reaches 692.8 mg / L. The method has the characteristics of high conversion efficiency, low production cost, convenience in preparation, wide industrial application prospect and the like.

The present invention discloses an antibacterial peptide cecropin A mutant, a recombinant expression vector, a Bacillus subtilis engineering bacterium and applications thereof. By modifying the antibacterial peptide cecropin A, the present invention screened mutants with antibacterial activities superior to those of the original cecropin A, and through the construction of a recombinant expression vector, heterologous expression was carried out using the biosafe strain Bacillus subtilis. Compared with the original antibacterial peptide cecropin A, the antibacterial activities of the mutants of the present invention were increased by 60% and 80% respectively. The present invention uses Bacillus subtilis as a secretion expression system to express the antibacterial peptide cecropin A mutant, which is conducive to large-scale industrial production and cost savings.

The present invention relates to means for recombinant manufacture. In particular, it relates to a recombinant bacterial cell comprising in its genome at least one essential gene the endogenous promoter of which has been replaced by a heterologous expression control sequence being inducible by an inducer molecule, such that the expression of said essential gene in the recombinant bacterial cell is dependent on the presence of said inducer molecule. Further contemplated are methods for generating the recombinant bacterial cell of the invention as well as a method for recombinant manufacture of a compound of interest. The present invention also provides for use the recombinant bacterial cell of the invention for recombinant manufacture of a compound of interest and a kit for manufacture of a compound of interest.

The invention provides a plasmid, an alpha-ketoglutaric acid engineering strain and a construction method and application of the alpha-ketoglutaric acid engineering strain, the strain is obtained by adopting a directional transformation method, transcriptional levels of katE, katG, sodB and grxA genes are up-regulated, a catR gene and a katA gene are heterologously expressed, a high-copy plasmid pETKG is carried, and the plasmid simultaneously expresses L-glutamate oxidase LGOX genes from Streptomyces sp and Chain A; the strain self-synthesized catalase eliminates H2O2 generated in the process of producing alpha-ketoglutaric acid through enzyme catalysis, engineering bacteria of L-glutamate oxidase LGOX expressed by pETKG plasmids are used for performing whole-cell catalysis on L-sodium glutamate to synthesize alpha-ketoglutaric acid, and the engineering strain is a high-level engineering strain capable of industrially and stably producing alpha-ketoglutaric acid.

The invention relates to pichia pastoris engineering bacteria and a preparation method thereof, and a method for producing cellooligosaccharide / disaccharide by using corn straws or wheat straws, and belongs to the technical field of genetic engineering. A cellulose incision enzyme gene and a cellulose excision enzyme gene are transferred into pichia pastoris engineering bacteria. The preparation method comprises the following steps: respectively inserting a cellulose incision enzyme gene and a cellulose excision enzyme gene into plasmids to construct expression vectors; transferring the expression vector into a competent cell of pichia pastoris GS115; and screening and identifying to obtain successfully transformed pichia pastoris engineering bacteria. According to the present invention, the pichia pastoris heterologous expression cellulose incision enzyme and cellulose excision enzyme are adopted to construct the pichia pastoris engineering strain capable of degrading the plant straw into the cellooligosaccharide / disaccharide, such that the reliable chassis strain is provided for the production of the starch from the cellulose;

The invention discloses a Pseudomonas putida high-yield rhamnolipid fermentation medium based on a step-by-step optimization strategy, an optimization method and application thereof, through the step-by-step strategy of component screening-single-factor optimization-multi-factor interactive optimization, a mixed carbon source and a mixed nitrogen source are taken as cores, and a Plackett-Burman test, a steepest climbing test and Box-Behnken response surface design are combined, so that the high-yield rhamnolipid fermentation medium for the pseudomonas putida high-yield rhamnolipid is obtained. Optimizing to obtain a special fermentation culture medium; two genetic engineering strains estA-2 and estA-3 are constructed by heterologous expression of key genes, and the supply and extracellular transport efficiency of a rhamnolipid synthesis precursor is enhanced; the recombinant strain is applied to the optimized culture medium, and efficient production of a shake flask and a 5L fermentation tank is achieved. The rhamnolipid yield of the estA-3 strain in a 5L fermentation tank reaches 35.59 + / -2.18 g / L, the conversion rate is 0.282 g / g, the productivity is 0.404 g / L / h, the rhamnolipid yield, the conversion rate and the productivity are obviously superior to those in the prior art, and a key technical support is provided for industrial production of rhamnolipid.

The application discloses a kind of fungal laccase mutant Lcc5-G and its expression strain and application.The application is based on the fungal laccase from Coprinopsis cinerea heterologous expression since, by site-directed mutagenesis, obtain mutant gene.After fungal induction expression containing mutant plasmid, obtain laccase mutant enzyme Lcc5-G with improved specific activity, stability and AFB1 removal efficiency.When ABTS is used as substrate, the specific activity of the mutant is improved by 3 times, and the stability of the mutant enzyme is improved to 5 times of the original enzyme at 45 DEG C and pH 7.The mutant has potential application value in oxidizing aflatoxin.

The invention belongs to the technical field of genetic engineering, and discloses a schizochytrium limacinum genetic engineering strain capable of reducing the proportion of DPA in grease and correspondingly increasing the content of DHA, a method and application of the schizochytrium limacinum genetic engineering strain. The strain is characterized in that an omega-3 desaturase gene derived from Platyneria dumeriii and / or Lepeophtheria salmonis is subjected to heterologous expression, so that the proportion of DPA (docosahexaenoic acid) in grease is reduced, and the content of DHA (docosahexaenoic acid) is correspondingly increased. According to the invention, by heterologous expression of Pd delta 19Des and Ls delta 19Des genes in schizochytrium limacinum, desaturation activity of two desaturases to a delta 19 position is verified, and conversion from omega-6 type DPA to omega-3 type DHA is effectively promoted.

The invention belongs to the technical field of gene engineering, and discloses recombinant yarrowia lipolytica as well as a construction method and application thereof. The recombinant yarrowia lipolytica PGA1 is obtained by carrying out heterologous expression on an arabidopsis thaliana inositol oxygenase gene and a pseudomonas syringae uronic acid dehydrogenase gene in the yarrowia lipolytica by utilizing a genetic engineering technical means. The recombinant yarrowia lipolytica PGA10 capable of efficiently synthesizing glucaric acid (GA) is finally obtained by further modifying six genes of related metabolic pathways of the recombinant yarrowia lipolytica PGA1. The GA yield of the PGA10 reaches 2233.24 mg / L and is 66.27 times that of PGA1, and the effect is remarkable. The invention not only constructs a GA synthesis method, but also defines the influence of related genes such as opi1 on GA synthesis, and provides reference for the research of heterologous synthesis of GA by microorganisms.

The invention discloses a marine-derived carboxylesterase and an application of the marine-derived carboxylesterase in degradation of PET (Polyethylene Terephthalate) plastics. The amino acid sequence of the enzyme is as shown in SEQ ID NO.1, experiments show that Tm of RbCE1 is 79.5 DEG C, 40 mM of depolymerization monomers can be generated after the enzyme treats low-crystallinity PET powder at 60 DEG C for 4 hours, the catalytic activity is equivalent to that of LCC, and good degradation activity is shown. Through escherichia coli heterologous expression, 0.4 g / L high expression quantity can be achieved in a shake flask, and the potential of remarkably reducing the enzyme production cost is achieved. Therefore, the novel carboxylesterase RbCE1 is suitable for degrading PET plastics and composite materials thereof, and is a potential enzyme preparation for efficient biological catalysis of a waste PET plastic waste enzymatic depolymerization recovery treatment process.

The invention discloses a progesterone 5beta-reductase mutant and application thereof in synthesis of 5beta-dihydrosteroid, relates to the technical field of biology, and in particular relates to application of gene mining and engineering modification of progesterone 5beta-reductase derived from bacteria in asymmetric synthesis of 5beta-dihydrosteroid. According to the progesterone 5 beta-reductase LpP5beta R disclosed by the invention, the progesterone 5 beta-reductase LpP5beta R from bacteria psoromais is obtained by a gene mining method, and the progesterone 5 beta-reductase LpP5beta R can be subjected to soluble expression in escherichia coli. The mutant obtained by mutating the wild type LpP5beta R has obviously improved catalytic ability on progesterone, and can efficiently catalyze delta 4-3-carbonyl steroids such as hydrocortisone, dinorcitol and the like to generate corresponding 5beta-dihydrosteroids. According to the invention, the technical bottlenecks of poor heterologous expression and low catalytic efficiency of plant-derived progesterone 5beta-reductase are solved, and a potential biocatalyst is provided for green manufacturing of 5beta-dihydrosteroid drugs.

The invention discloses cloning and functional identification of a liverwort flavone synthetase gene, and belongs to the technical field of gene engineering. According to the invention, one flavone synthase I (flavones synthase I, FNS I) is obtained through screening, and is named as MpFNS I; a prokaryotic expression system is utilized, an MpFNS I recombinant protein is obtained through purification, and the result of in-vitro enzyme activity identification shows that an MpFNS I enzyme substrate is relatively wide in selectivity, has a flavone synthetase function and also has a part of flavonol synthetase function. The liverwort flavonoid has a significant difference on affinity and catalytic efficiency of different substrates, the in-vivo function of the liverwort flavonoid is verified by utilizing heterologous expression of arabidopsis thaliana, experimental evidence is provided for comprehensively analyzing a biosynthetic pathway of the liverwort flavonoid, and meanwhile, a research target is provided for deeply researching a mechanism for synthesizing and responding to UV-B radiation of the liverwort flavonoid.

The invention discloses application of a scutellaria baicalensis SbMYB8 gene in promotion of anthocyanin synthesis, the application comprises the steps that the SbMYB8 gene is constructed to a plant expression vector and introduced into plant cells to promote synthesis of plant anthocyanin, and the nucleotide sequence of the SbMYB8 gene is shown as SEQ ID NO: 1, or the amino acid sequence coded by the SbMYB8 gene is shown as SEQ ID NO: 2. The MYB transcription factor SbMYB8 gene with the function of promoting anthocyanin synthesis is cloned and identified from the scutellaria baicalensis for the first time, and the blank of a transcription regulation mechanism for regulating anthocyanin metabolism in the scutellaria baicalensis is filled; the SbMYB8 gene can significantly promote accumulation of anthocyanin in various plants, shows good heterologous expression stability and adaptability, and has wide cross-species application potential.

The application provides a recombinant Aspergillus niger with high trans-aconitic acid yield and a construction method and application thereof, and relates to the technical field of genetic engineering. Specifically, the recombinant Aspergillus niger heterogeneously expresses aconitate isomerase TbrA and / or Adi1. The application provides a new engineering strain for efficient production of trans-aconitic acid, solves the source predicament of needing plant extraction or needing chemical synthesis in traditional production of trans-aconitic acid, and greatly improves the yield of trans-aconitic acid.

The invention provides a resveratrol production strain and a construction method and application thereof, according to the strain, tyrAfbr, tyrB, aroE, pntAB, ompF, aroGfbr and accA genes are up-regulated, a carbon storage regulation factor csrA gene is knocked out, the transcriptional level of a fabF gene is down-regulated through a BBaJ23113 promoter, a TAL gene from Rhodotorula glutinis, a 4CL gene from Arabidopsis thaliana and an STS gene from Vitis vinifera are heterologously expressed on a genome, and the strain carries a high-copy plasmid PET-28a-WAL at the same time; the strain takes glucose as a carbon source, resveratrol is efficiently and stably synthesized from the beginning by adopting a fermentation method, the production cost is low, and efficient production of resveratrol is realized.

The invention relates to an alcohol oxidase mutant and application thereof. Specifically disclosed are a mutant derived from Gloeophyllum trabeum alcohol oxidase (GtrAOX-M2), a nucleic acid encoding the alcohol oxidase mutant, a recombinant expression vector containing the nucleic acid, a recombinant expression transformant containing the recombinant expression vector, a preparation method of a recombinant alcohol oxidase catalyst, and a preparation method of the recombinant alcohol oxidase catalyst. The invention also discloses a method for catalyzing methanol to be oxidized into formaldehyde by using the alcohol oxidase, the alcohol oxidase mutant or the recombinant alcohol oxidase catalyst. The activity of the alcohol oxidase mutant provided by the invention is obviously improved compared with that of a female parent, and the enzyme is high in activity and can be heterologously expressed in escherichia coli, so that the alcohol oxidase mutant can efficiently oxidize methanol into formaldehyde and oxidize the methanol into formaldehyde by a one-carbon unit biological utilization methanol enzyme method; wide application prospects are shown in the aspect of subsequent conversion and generation of polyhydric alcohols.

The invention provides a strain for producing L-isoleucine as well as a construction method and application thereof. The strain is obtained by modifying a chassis strain escherichia coli XX12 by utilizing a metabolic engineering modification method, ldhA, adhE and pflB genes are deleted, the transcriptional levels of aspC, pykF, pntAB, ppK, ppc, ilvAfbr, ilvIHfbr and ygaZH genes are up-regulated, the transcriptional level of a leuA gene is down-regulated, a bcd gene derived from B.subtilis 168 and ppnK and cysK genes derived from Cornebacterium glutamicum ATCC 13032 are heterologously expressed, and the strain has the advantages that the strain can be used for producing L-isoleucine; the method has the advantages of no need of adding resistant substances, good L-isoleucine synthesis capability, short fermentation period by using glucose as a carbon source, and good economic benefit and industrial application value.

The invention discloses an escherichia coli engineering bacterium for producing 5-ALA by an anaerobic fermentation method as well as a construction method and a fermentation method of the escherichia coli engineering bacterium. The engineering bacterium is constructed by taking escherichia coli (E. coli) ZX11 as a host and performing heterologous expression on 5-ALA synthetase (HemA) from rhodobacter capsulatus. In order to improve the soluble expression level of the HemA, the HemA is subjected to fusion expression with a maltose binding protein (MBP) tag. Furthermore, the engineering bacterium can also heterologously express a visual purple protein (PR) fused with the MBP tag. The construction method of the engineering bacteria comprises the following steps: sequentially cloning the MBP-HemA fusion gene and the PR gene to an expression vector and transferring into host bacteria. By utilizing the engineering bacteria provided by the invention, anaerobic fermentation can be carried out in a culture medium taking glucose as a carbon source, and 5-ALA can be efficiently synthesized. The invention provides an effective way for realizing green and energy-saving anaerobic biological manufacturing of 5-ALA.

The invention discloses a BvM14-TAG2 gene and application thereof, and relates to the technical field of biological genes. The CDS sequence of the BvM14-TAG2 gene disclosed by the invention is as shown in SEQ ID NO. 1. The amino acid sequence of the protein coded by the BvM14-TAG2 gene is as shown in SEQ ID No. 2. According to the invention, the BvM14-TAG2 gene is utilized to construct an arabidopsis thaliana plant for heterologous expression of the BvM14-TAG2 gene, stress treatment is carried out, and the seed germination rate and root length of the plant for heterologous expression of the BvM14-TAG2 gene are both superior to those of a wild type; the biomass of a plant for heterologous expression of the BvM14-TAG2 gene is higher than that of a wild plant; the result shows that the BvM14-TAG2 gene can promote the growth and development of the plant under the salt stress, and can improve the adaptive capacity of the BvM14-TAG2 transgenic plant under the salt stress.

The invention belongs to the technical field of genetic engineering, and discloses a schizochytrium limacinum genetic engineering strain for enhancing supply of acetyl coenzyme A and efficiently producing astaxanthin, a method and application of the schizochytrium limacinum genetic engineering strain. By heterologous expression of a beta-carotene ketolase CrtO gene and a beta-carotene hydroxylase CrtZ gene from haematococcus pluvialis, synthesis of astaxanthin from beta-carotene in schizochytrium limacinum is promoted, so that the content of astaxanthin in schizochytrium limacinum HX-308 is increased. According to the invention, the endogenous AACT gene and heterologous CrtO, CrtZ, PK and PTA genes of schizochytrium limacinum are integrated into an original strain through homologous recombination, and the obtained strain still keeps genetic stability after multiple subculture, has the capability of efficiently synthesizing astaxanthin, effectively improves the yield of astaxanthin, and lays a foundation for large-scale industrial production.

The invention discloses a pennisetum purpureum CpERF37 transcription factor and cold-resistant application thereof, and relates to the technical field of plant genetic engineering. According to the invention, the Pennisetum purpureum-sourced ERF transcription factor CpERF37 is cloned and functionally identified for the first time, and heterologous expression verification in a model plant arabidopsis thaliana proves that the Pennisetum purpureum-sourced ERF transcription factor CpERF37 has a remarkable effect on improving the low-temperature tolerance of plants, and functional gene resources related to low-temperature stress response are enriched. The method not only has theoretical innovation, but also has a definite application prospect, and can provide effective gene resources and technical support for cold-resistant improvement of pasture and crops.

The invention discloses a larimichthys crocea IL-22 recombinant protein which is a larimichthys crocea IL-22 mature peptide sequence, and the amino acid sequence of the larimichthys crocea IL-22 recombinant protein is as shown in SEQ ID NO. 2. The recombinant protein is obtained by amplifying and coding gene segments of amino acids from 31st to 190th positions of large yellow croaker IL-22 mature peptide through PCR (Polymerase Chain Reaction), and transforming escherichia coli for heterologous expression. The larimichthys crocea IL-22 recombinant protein can activate a transcription factor STAT3 in a larimichthys crocea head kidney cell line (LYCK) and also can induce expression of inflammatory factors (IL-1beta, IL-6 and IL-8) in immune organs of larimichthys crocea, so that the larimichthys crocea IL-22 recombinant protein has an obvious immunoregulation effect and shows a good application prospect in research and development of aquatic immunomodulators.

The invention relates to bacillus subtilis for expressing bovine-derived beta-lactoglobulin and a construction method of the bacillus subtilis, and belongs to the technical field of microorganisms. According to the bacillus subtilis provided by the invention, bacillus subtilis BSF01 is used as a host, a constitutive promoter P566 is used for heterologous expression of a bovine-derived beta-lactoglobulin coding gene optimized by a codon, the yield of beta-lactoglobulin obtained by shake flask fermentation for 30 hours reaches 0.37 g / L, the yield of beta-lactoglobulin obtained by 3L fermentation tank fermentation for 44 hours reaches 3.06 g / L, the production intensity is 0.07 g / L / h, and the production cost is low. The bovine-derived beta-lactoglobulin is efficiently and insolubly expressed in a food safety level strain, the food industrial application of the beta-lactoglobulin is promoted, and the problems of food safety and excessive glycosylation possibly existing in heterologous expression of the beta-lactoglobulin in the prior art are solved.

The invention discloses a salt stress resistant gene BvAGER1 and application thereof, and relates to the technical field of biological genes, in particular to the salt stress resistant gene BvAGER1 and the application field thereof. According to the salt stress resistant gene BvAGER1 disclosed by the invention, a CDS (Coding Sequence) sequence of the gene BvAGER1 is as shown in SEQ ID NO. 1. The amino acid sequence of the protein coded by the salt stress resistant gene BvAGER1 is as shown in SEQ ID No. 2. The invention also discloses an application of the salt stress resistant gene BvAGER1 in improving the salt tolerance of plants. The BvAGER1 gene in a BvAGER1 gene heterologous overexpression plant prepared by utilizing the BvAGER1 gene can be stably inherited, the heterologous expression BvAGER1 gene can relieve the inhibition effect of salt stress on plant germination, and the inhibition effect on growth of roots and leaves of the BvAGER1 gene heterologous overexpression plant can be obviously weakened under the salt stress; the adaptive capacity under the salt stress is improved.

The invention provides application of Ustilaginoidea virens secretory protein UvWMT and a coding gene thereof in prevention and control of plant diseases. The gene sequence of the Ustilaginoidea virens secretory protein UvWMT is researched, found and cloned for the first time, and the new application of the Ustilaginoidea virens secretory protein UvWMT in disease-resistant breeding of crops such as rice is developed. Research finds that the protein is a key pathogenic factor in the process that rice is infected by ustilaginoidea virens, heterologous expression experiments further find that heterologous expression of UvWMT in rice can significantly improve the resistance of crops to various pathogenic bacteria such as ustilaginoidea virens, magnaporthe oryzae and xanthomonas oryzae, and has no significant difference in key agronomic traits compared with a wild type. An important theoretical basis is provided for rice disease-resistant breeding, crops can be safely and effectively endowed with broad-spectrum disease resistance by heterologous expression of the gene, and the gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.