8 results about "Alcohol oxidase" patented technology

The invention relates to an alcohol oxidase mutant and application thereof. Specifically disclosed are a mutant derived from Gloeophyllum trabeum alcohol oxidase (GtrAOX-M2), a nucleic acid encoding the alcohol oxidase mutant, a recombinant expression vector containing the nucleic acid, a recombinant expression transformant containing the recombinant expression vector, a preparation method of a recombinant alcohol oxidase catalyst, and a preparation method of the recombinant alcohol oxidase catalyst. The invention also discloses a method for catalyzing methanol to be oxidized into formaldehyde by using the alcohol oxidase, the alcohol oxidase mutant or the recombinant alcohol oxidase catalyst. The activity of the alcohol oxidase mutant provided by the invention is obviously improved compared with that of a female parent, and the enzyme is high in activity and can be heterologously expressed in escherichia coli, so that the alcohol oxidase mutant can efficiently oxidize methanol into formaldehyde and oxidize the methanol into formaldehyde by a one-carbon unit biological utilization methanol enzyme method; wide application prospects are shown in the aspect of subsequent conversion and generation of polyhydric alcohols.

The invention discloses 10-hydroxydecanoic acid as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, Candida lipolytica is taken as a fermentation thallus, the Candida lipolytica contains alkane monooxygenase (CYP450), alcohol dehydrogenase (ADH), fatty alcohol oxidase (FAO) and fatty aldehyde dehydrogenase (FALDH), amplification and activation are performed on the Candida lipolytica in a seed culture medium, and the Candida lipolytica is obtained. The method comprises the following steps: inoculating a seed solution into a liquid culture medium, enabling a strain to adapt to a culture environment in advance, reducing strain death caused by environmental mutation after inoculation, inoculating the seed solution into the liquid culture medium, culturing until OD600 is 45-55, realizing high-density enrichment of the strain, being beneficial to increasing the content of CYP450, ADH, FAO and FALDH, adding decane and Tween 80, and enabling the Tween 80 to be capable of increasing the permeability of a cell membrane and increasing the reaction rate, the CYP450 is used for catalyzing decane to carry out hydroxylation, and ADH, FAO and FALDH are used for carrying out oxidation, so that the high-purity 10-hydroxydecanoic acid is obtained.

The present invention relates to a method for the expression of genes belonging to different organisms (bacteria, fungi, plants, animals and humans) in P. pastoris cells, comprising the following processing steps;i. Transfer of the gene to be expressed into the plasmid carrying the alcohol oxidase 1 (AOX1) promoterii. Cloning of the plasmid carrying the geneiii. Transfer of the recombinant plasmid carrying the AOX1 promoter and gene into the expression hostiv. Induction of the AOX1 promoter with farnesol and consequent expression of the heterologous gene.The present invention will be used in the field of recombinant protein production.

This invention relates to the field of biotechnology, specifically to an inositol oxidase mutant and its application in the production of glucuronic acid. The inositol oxidase mutant provided by this invention is obtained by point mutation of the amino acid sequence shown in the wild-type inositol oxidase. Compared with the wild-type enzyme, the inositol oxidase mutant constructed by this invention exhibits a significant improvement in catalytic efficiency. Furthermore, this invention also provides an engineered strain with site-directed mutagenesis and knockout of the UxaC gene in the chassis cell genome. This strain exhibits higher catalytic activity towards the substrate, with a catalytic efficiency increased by 74.44% compared to the wild-type enzyme. Even at high substrate concentrations, the conversion rate can still reach over 92.09%, with a production intensity as high as 13.23 g / L / h. It possesses extremely strong substrate tolerance and production intensity, significantly reducing the production cost of glucuronic acid and demonstrating significant industrial application potential.

This invention relates to the fields of enzyme engineering and biocatalysis, specifically to a mutant of the alcohol oxidase IgAOX and its applications. The amino acid sequence of the mutant IgAOX is as follows: The amino acid sequence shown in SEQ ID NO: 1 is modified by replacing V at position 229 with A, or S at position 271 with G, or K at position 297 with R, or K at position 297 with R, while simultaneously replacing Y at position 605 with M, or S at position 271 with G, and A at position 227 with S, or K at position 297 with R, and A at position 227 with S. This invention yields mutants with improved catalytic activity and thermal stability. These mutants enable their application in biosynthesis and biocatalysis, providing new ideas and a technological foundation for the green and environmentally friendly synthesis of chemical raw materials.

The application belongs to the technical field of nanomaterials, and provides a CaPhy-NAD nanoenzyme, a preparation method, application and an alcoholism treatment preparation thereof. The CaPhy-NAD nanoenzyme is prepared by using calcium phytate nanoparticles as a carrier and in-situ loading of ethanol oxidase, acetaldehyde dehydrogenase, horseradish peroxidase and coenzyme NAD. 2+ The CaPhy-NAD nanoenzyme can effectively activate the catalytic activity of acetaldehyde dehydrogenase, and the three enzymes can have a high-efficiency confined cascade reaction in the pore channel of the calcium phytate nanoparticles, so that the recycling of the coenzyme NAD is realized, and the three types of alcohol metabolism-related toxic substances, i.e., ethanol, acetaldehyde and hydrogen peroxide, can be simultaneously removed. The CaPhy-NAD nanoenzyme prepared by the application has excellent catalytic activity and good biological safety, and can be orally administered, thereby providing a new technical idea and solution for the prevention and treatment of alcoholism.

Disclosed are a genetically engineered bacterium catalyzing the production of a quinoline compound, and a use thereof. The genetically engineered bacterium expresses monoamine oxidase and an enzyme capable of catalyzing alcohol oxidation, the enzyme capable of catalyzing alcohol oxidation being selected from alcohol oxidase or alcohol dehydrogenase. It has been unexpectedly discovered that monoamine oxidase coupled to alcohol dehydrogenase or alcohol oxidase can catalyze the preparation of quinoline from amino alcohol substrates, which fills the gap that current biocatalysis methods cannot synthesize amino alcohol substrates into quinoline compounds.

The application discloses a genetically engineered bacterium for producing cinnamic acid and derivatives thereof, a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium co-expresses vanillyl alcohol oxidase and oxidoreductase. The genetically engineered bacterium can be used as a biological catalyst to convert a substrate into cinnamic acid and derivatives thereof. The method for producing cinnamic acid and derivatives thereof has the advantages of high production efficiency, short reaction period, low cost and good industrial application prospect.