3 results about "Alcohol oxidase" patented technology

This invention relates to the field of biotechnology, specifically to an inositol oxidase mutant and its application in the production of glucuronic acid. The inositol oxidase mutant provided by this invention is obtained by point mutation of the amino acid sequence shown in the wild-type inositol oxidase. Compared with the wild-type enzyme, the inositol oxidase mutant constructed by this invention exhibits a significant improvement in catalytic efficiency. Furthermore, this invention also provides an engineered strain with site-directed mutagenesis and knockout of the UxaC gene in the chassis cell genome. This strain exhibits higher catalytic activity towards the substrate, with a catalytic efficiency increased by 74.44% compared to the wild-type enzyme. Even at high substrate concentrations, the conversion rate can still reach over 92.09%, with a production intensity as high as 13.23 g / L / h. It possesses extremely strong substrate tolerance and production intensity, significantly reducing the production cost of glucuronic acid and demonstrating significant industrial application potential.

This invention relates to the fields of enzyme engineering and biocatalysis, specifically to a mutant of the alcohol oxidase IgAOX and its applications. The amino acid sequence of the mutant IgAOX is as follows: The amino acid sequence shown in SEQ ID NO: 1 is modified by replacing V at position 229 with A, or S at position 271 with G, or K at position 297 with R, or K at position 297 with R, while simultaneously replacing Y at position 605 with M, or S at position 271 with G, and A at position 227 with S, or K at position 297 with R, and A at position 227 with S. This invention yields mutants with improved catalytic activity and thermal stability. These mutants enable their application in biosynthesis and biocatalysis, providing new ideas and a technological foundation for the green and environmentally friendly synthesis of chemical raw materials.

The application belongs to the technical field of nanomaterials, and provides a CaPhy-NAD nanoenzyme, a preparation method, application and an alcoholism treatment preparation thereof. The CaPhy-NAD nanoenzyme is prepared by using calcium phytate nanoparticles as a carrier and in-situ loading of ethanol oxidase, acetaldehyde dehydrogenase, horseradish peroxidase and coenzyme NAD. 2+ The CaPhy-NAD nanoenzyme can effectively activate the catalytic activity of acetaldehyde dehydrogenase, and the three enzymes can have a high-efficiency confined cascade reaction in the pore channel of the calcium phytate nanoparticles, so that the recycling of the coenzyme NAD is realized, and the three types of alcohol metabolism-related toxic substances, i.e., ethanol, acetaldehyde and hydrogen peroxide, can be simultaneously removed. The CaPhy-NAD nanoenzyme prepared by the application has excellent catalytic activity and good biological safety, and can be orally administered, thereby providing a new technical idea and solution for the prevention and treatment of alcoholism.