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Ethanol concentration detection kit by using circulatory enzyme method and preparation method thereof

A technology for detecting kits and ethanol concentration, applied in the field of medical testing, can solve the problems of affecting accuracy, color fading, low reaction sensitivity, etc., and achieve the effects of easy portability, fast reaction speed and high sensitivity

Inactive Publication Date: 2013-01-30
CHINA MARITIME POLICE ACADEMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many portable ethanol detection reagent strips on the market, but they all have the following defects: (1) the color of the reagent will fade quickly after the reaction; (2) the reaction sensitivity is low, and the accumulation of the generated products inhibits the reaction , affecting the accuracy of detection

Method used

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  • Ethanol concentration detection kit by using circulatory enzyme method and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The ethanol concentration detection kit of this embodiment is composed of a reagent strip and a standard comparison card, wherein the reagent strip is a filter paper strip with a width of 0.5-1 cm, and the filter paper strip contains solid-phase alcohol oxidase, alcohol dehydrogenase, and Prototype coenzyme I, horseradish peroxidase, 4-AAP, aniline analogs and color stabilizer, standard comparison card includes color comparison areas of different ethanol concentrations. The standard comparison card includes five color comparison areas with ethanol concentrations of 0 mg / ml, 0.2 mg / ml, 0.8 mg / ml, 1.5 mg / ml and 3.0 mg / ml respectively.

[0038] The preparation method of the ethanol concentration detection kit comprises the preparation of reagent strips and the preparation of standard comparison cards, wherein:

[0039] The preparation of the reagent strip comprises the following steps:

[0040] (1) Preparation of alcohol oxidase, horseradish peroxidase and alcohol dehydro...

Embodiment 2

[0052] The ethanol concentration detection kit of this embodiment is composed of a reagent strip and a standard comparison card, wherein the reagent strip is a filter paper strip with a width of 0.5-1 cm, and the filter paper strip contains solid-phase alcohol oxidase, alcohol dehydrogenase, and Prototype coenzyme I, horseradish peroxidase, 4-AAP, aniline analogs and color stabilizer, standard comparison card includes color comparison areas of different ethanol concentrations. The standard comparison card includes five color comparison areas with ethanol concentrations of 0 mg / ml, 0.2 mg / ml, 0.8 mg / ml, 1.5 mg / ml and 3.0 mg / ml respectively.

[0053] The preparation method of the ethanol concentration detection kit comprises the preparation of reagent strips and the preparation of standard comparison cards, wherein:

[0054] The preparation of the reagent strip comprises the following steps:

[0055] (1) Preparation of alcohol oxidase, horseradish peroxidase and alcohol dehydro...

Embodiment 3

[0067] The ethanol concentration detection kit of this embodiment is composed of a reagent strip and a standard comparison card, wherein the reagent strip is a filter paper strip with a width of 0.5-1 cm, and the filter paper strip contains solid-phase alcohol oxidase, alcohol dehydrogenase, and Prototype coenzyme I, horseradish peroxidase, 4-AAP, aniline analogs and color stabilizer, standard comparison card includes color comparison areas of different ethanol concentrations. The standard comparison card includes five color comparison areas with ethanol concentrations of 0 mg / ml, 0.2 mg / ml, 0.8 mg / ml, 1.5 mg / ml and 3.0 mg / ml respectively.

[0068] The preparation method of the ethanol concentration detection kit comprises the preparation of reagent strips and the preparation of standard comparison cards, wherein:

[0069] The preparation of the reagent strip comprises the following steps:

[0070] (1) Preparation of alcohol oxidase, horseradish peroxidase and alcohol dehydro...

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Abstract

The invention provides an ethanol concentration detection kit by using a circulatory enzyme method and a preparation method thereof. The kit comprises a reagent strip and a standard comparison card, wherein the reagent strip is of a filter paper strip with the width of 0.5-1cm, the filter paper strip contains immobilized alcohol oxidase, alcohol dehydrogenase, reduction type coenzyme I, horseradish peroxidase, 4-acetoxyazetidinone, an aniline analog and a coloring stabilizer, and the standard comparison card comprises coloring comparison area with different ethanol concentrations. When the kit is used for detecting the ethanol concentration, the operation is simple and sanitary, the speed is fast, the sensitivity is high and the stability is high; and the kit is easy to carry and particularly suitable for field detection.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a kit for detecting ethanol content in saliva by a circulating enzyme method and a preparation method thereof. Background technique [0002] In recent years, criminal cases and traffic accidents caused by drinking have been on the rise. On May 1, 2011, the "Criminal Law Amendment of the People's Republic of China (VIII)" was officially implemented. Drunk driving was held criminally responsible for the crime of dangerous driving. The topic of drunk driving has once again become the focus of attention. [0003] At present, many countries have formulated the relationship between blood ethanol concentration and traffic accident responsibility determination, and established a variety of methods that can detect blood ethanol content, such as GM / MS method. The equipment is expensive, the detection cost is high, special training is required, and there are shortcomings such as th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 王金堂高伟良王珂王哲元
Owner CHINA MARITIME POLICE ACADEMY
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