9results about How to "Remove inhibition" patented technology

The invention belongs to the technical field of biology, and particularly relates to a reagent for detecting pathogenic genes as well as a preparation method and application thereof. The invention finds that a biological sample is enriched through broad-spectrum captured protein, the captured protein is labeled with biotin, pathogenic microorganisms are separated from the biological sample through high-density streptavidin coated magnetic beads, the pathogenic microorganisms are split after pure culture, pathogenic genes are detected after nucleic acid is extracted, and the turnover time is shortened to 4 hours. The precise distribution of nucleic acid is realized through an annular array reaction cavity preloaded with 32 freeze-dried LAMP reagents in combination with centrifugal force driving. The single chip synchronously detects more than or equal to 30 major pathogen genes / drug-resistant genes, covers common bloodstream infection pathogens, and breaks through the existing POCT flux bottleneck. According to the present invention, with the kit, the blood matrix inhibition can be eliminated, the pathogen concentration can be rapidly increased (the detection threshold value can be reached after culture), the positive detection rate can be increased to the clinical requirement level (gt, 90%), and the pathogen detection limit in the blood sample can meet the clinical critical infection early diagnosis requirement of less than or equal to 10 CFU / mL;

The application relates to the technical field of satellite communication, and particularly discloses a space-ground integrated anti-interference method and system based on multi-satellite cooperation, which comprises the following steps: S1, a ground station sends a communication signal, and an interference station sends an interference signal; S2, a cooperative satellite and a communication satellite simultaneously receive the communication signal and the interference signal, obtain a disturbed signal, and transmit the disturbed signal to a receiving ground station; S3, the receiving ground station performs Taylor series expansion on a delayed communication signal, separates a delay difference of the disturbed signal from a signal waveform, and obtains a disturbed signal of a single satellite after eliminating the delay difference and a disturbed total signal of all satellites; S4, the receiving ground station performs whitening processing on the disturbed total signal, and obtains a whitened signal; and S5, the receiving ground station rotates the whitened signal, extracts an estimated spread signal vector from the whitened signal, and identifies an estimated communication signal. The application effectively suppresses strong external interference and realizes a method and system for reliably recovering a communication signal.

The application discloses an anaerobic biogas preparation device based on kitchen garbage, and relates to the technical field of kitchen garbage treatment. The device comprises a squeezing box, a feeding mechanism capable of crushing and treating the kitchen garbage is connected to the upper end of the squeezing box, a separation mechanism for separating the solid and liquid of the kitchen garbage is installed in the squeezing box, a liquid discharge mechanism capable of separating and discharging the grease liquid and the non-grease liquid of the kitchen garbage filtrate is arranged below the separation mechanism, a conveying auger, and a pyrolysis furnace. The conveying auger is communicated with the inside of the pyrolysis furnace through a connecting pipe. The squeezing box, the conveying auger and the pyrolysis furnace are matched, the high-grease liquid is separately separated by the liquid discharge mechanism, and only the non-grease liquid is sent into the anaerobic reactor, so that the inhibition of grease on methanogens is eliminated from the source, the pyrolysis condensate and the non-grease liquid are cooperatively fermented, the utilization rate of the reaction substrate is improved, and the biogas yield is further improved.

This application provides a standard working time dynamic self-tuning method based on the PID algorithm, applied in the field of production management technology. It obtains a dataset of measured time consumption for a target task over multiple execution cycles, preprocesses the dataset to remove outliers, and obtains an effective working time feature set. The logical deviation between this effective working time feature set and the current target reference value is determined, and a PID control algorithm is executed based on this deviation to achieve precise control of the working time data. During the iterative update of the target reference value, an ELM prediction model is used to dynamically adjust the control parameters of the PID control algorithm based on historical deviation trends. Finally, when the fluctuation range of the target reference value and the prediction error meet a preset threshold, the final standard working time is output, ensuring the stability and accuracy of the standard working time. This method has the advantage of dynamically adapting to changes in the production environment and improving the accuracy and stability of the standard working time.

This invention discloses an improved multi-stage ultra-long gravity heat pipe, belonging to the technical field of ultra-long gravity heat pipes. The improved multi-stage ultra-long gravity heat pipe includes a heat pipe body, a support mechanism fixedly installed on the outer side of the heat pipe body, several steam pools fixedly installed on one side of the inner wall of the heat pipe body, and several two-phase flow mechanisms fixedly installed on the other side of the inner wall of the heat pipe body. A return liquid pipe extending into the two-phase flow mechanism is fixedly installed inside the heat pipe body. Each of the two-phase flow mechanisms includes a buffer pool and an overflow pipe. One side of the bottom of the buffer pool is fixedly connected to the top of the overflow pipe, and the bottom of the buffer pool has several open liquid tanks. By setting up two-phase flow mechanisms, the working fluid return is achieved by gravity, requiring no external power input. The heat transfer coefficient is higher than that of copper, and groundwater is not extracted, avoiding geological subsidence, brine scaling, water resource consumption, and the phenomenon of decreased or even failed heat transfer performance.

The application discloses a kind of citric acid-microbial inoculant coproduction process methods.The process method described in the application is as follows: (1) citric acid fermentation liquor is subjected to plate and frame filtration treatment, and mycelium and citric acid feed liquid are separated, the citric acid feed liquid is neutralized, and calcium citrate and raffinate are obtained after filtration, calcium citrate enters subsequent extraction process, and citric acid is prepared; and raffinate enters evaporator for concentration, and concentrated liquid and condensate are obtained; (2) the condensate is used as citric acid production raw material mixing water, and the concentrated liquid is used for culturing microorganisms after pretreatment, and microbial inoculant is obtained; (3) then the microbial inoculant is centrifuged, and concentrated microbial inoculant and tail liquid are obtained, the concentrated microbial inoculant is added with protective agent and carrier, and microbial inoculant is obtained by spray drying.The method described in the application uses citric acid production raffinate as microbial culture nutrient source, realizes high-value and resource utilization of nutrient substances in raffinate, significantly reduces water consumption, and reduces sewage discharge.

The present disclosure relates to a protein specifically binding to PD-1 and a pharmaceutical use thereof. Specifically, the present disclosure provides a protein specifically binding to PD-1, a protein specifically binding to PD-1 / PVRIG / TIGIT, and a use thereof in the treatment of a disease (such as cancer).

The invention belongs to the technical field of biological fermentation and crystallization separation, discloses a method and a system for producing isomaltulose by coupling double-bacterium synergistic fermentation with gradient temperature control crystallization, and solves the problem that glucose generates feedback inhibition on sucrose isomerase in a fermentation stage. The method comprises a fermentation stage and a crystallization stage, a double-bacterium synergistic fermentation system of recombinant bacillus subtilis engineering bacteria and recombinant pichia pastoris engineering bacteria is adopted in the fermentation stage, and the inoculation ratio of the recombinant bacillus subtilis engineering bacteria to the recombinant pichia pastoris engineering bacteria is 3: 1-5: 1; in the crystallization stage, a gradient temperature control-ultrasonic-assisted coupling crystallization process is adopted, after the fermentation liquor is subjected to concentration pretreatment, the temperature is reduced to 25-28 DEG C at the speed of 0.5-0.8 DEG C / h, and heat preservation and ultrasonic treatment are performed; and cooling to 15-18 DEG C at the speed of 0.3-0.5 DEG C / h, preserving heat and carrying out ultrasonic treatment. The method provided by the invention realizes efficient production and accurate purification of isomaltulose.

The invention provides a light diesel engine tail gas after-treatment system and a control method thereof, which can solve the problems of high cold start emission, catalyst poisoning caused by HC, N2O generation and difficulty in reaching the standard of PN10 emission in the prior art, meet the requirements of a new generation of emission regulations, and improve the emission efficiency. Comprising a ccDOC unit, a first urea injection unit, a ccSCR unit, a DPF unit, a second urea injection unit, a second SCR unit and an ASC unit which are sequentially arranged in the tail gas direction of an engine. The controller is electrically connected with the first urea spraying unit and the second urea spraying unit; and the controller controls the first urea injection unit to inject a first urea amount U1 into the tail gas in front of the ccSCR unit and controls the second urea injection unit to inject a second urea amount U2 into the tail gas in front of the second SCR unit according to a preset control method.