5results about How to "Remove inhibition" patented technology

The invention belongs to the technical field of biology, and particularly relates to a reagent for detecting pathogenic genes as well as a preparation method and application thereof. The invention finds that a biological sample is enriched through broad-spectrum captured protein, the captured protein is labeled with biotin, pathogenic microorganisms are separated from the biological sample through high-density streptavidin coated magnetic beads, the pathogenic microorganisms are split after pure culture, pathogenic genes are detected after nucleic acid is extracted, and the turnover time is shortened to 4 hours. The precise distribution of nucleic acid is realized through an annular array reaction cavity preloaded with 32 freeze-dried LAMP reagents in combination with centrifugal force driving. The single chip synchronously detects more than or equal to 30 major pathogen genes / drug-resistant genes, covers common bloodstream infection pathogens, and breaks through the existing POCT flux bottleneck. According to the present invention, with the kit, the blood matrix inhibition can be eliminated, the pathogen concentration can be rapidly increased (the detection threshold value can be reached after culture), the positive detection rate can be increased to the clinical requirement level (gt, 90%), and the pathogen detection limit in the blood sample can meet the clinical critical infection early diagnosis requirement of less than or equal to 10 CFU / mL;

This application provides a standard working time dynamic self-tuning method based on the PID algorithm, applied in the field of production management technology. It obtains a dataset of measured time consumption for a target task over multiple execution cycles, preprocesses the dataset to remove outliers, and obtains an effective working time feature set. The logical deviation between this effective working time feature set and the current target reference value is determined, and a PID control algorithm is executed based on this deviation to achieve precise control of the working time data. During the iterative update of the target reference value, an ELM prediction model is used to dynamically adjust the control parameters of the PID control algorithm based on historical deviation trends. Finally, when the fluctuation range of the target reference value and the prediction error meet a preset threshold, the final standard working time is output, ensuring the stability and accuracy of the standard working time. This method has the advantage of dynamically adapting to changes in the production environment and improving the accuracy and stability of the standard working time.

This invention discloses an improved multi-stage ultra-long gravity heat pipe, belonging to the technical field of ultra-long gravity heat pipes. The improved multi-stage ultra-long gravity heat pipe includes a heat pipe body, a support mechanism fixedly installed on the outer side of the heat pipe body, several steam pools fixedly installed on one side of the inner wall of the heat pipe body, and several two-phase flow mechanisms fixedly installed on the other side of the inner wall of the heat pipe body. A return liquid pipe extending into the two-phase flow mechanism is fixedly installed inside the heat pipe body. Each of the two-phase flow mechanisms includes a buffer pool and an overflow pipe. One side of the bottom of the buffer pool is fixedly connected to the top of the overflow pipe, and the bottom of the buffer pool has several open liquid tanks. By setting up two-phase flow mechanisms, the working fluid return is achieved by gravity, requiring no external power input. The heat transfer coefficient is higher than that of copper, and groundwater is not extracted, avoiding geological subsidence, brine scaling, water resource consumption, and the phenomenon of decreased or even failed heat transfer performance.

The invention belongs to the technical field of biological fermentation and crystallization separation, discloses a method and a system for producing isomaltulose by coupling double-bacterium synergistic fermentation with gradient temperature control crystallization, and solves the problem that glucose generates feedback inhibition on sucrose isomerase in a fermentation stage. The method comprises a fermentation stage and a crystallization stage, a double-bacterium synergistic fermentation system of recombinant bacillus subtilis engineering bacteria and recombinant pichia pastoris engineering bacteria is adopted in the fermentation stage, and the inoculation ratio of the recombinant bacillus subtilis engineering bacteria to the recombinant pichia pastoris engineering bacteria is 3: 1-5: 1; in the crystallization stage, a gradient temperature control-ultrasonic-assisted coupling crystallization process is adopted, after the fermentation liquor is subjected to concentration pretreatment, the temperature is reduced to 25-28 DEG C at the speed of 0.5-0.8 DEG C / h, and heat preservation and ultrasonic treatment are performed; and cooling to 15-18 DEG C at the speed of 0.3-0.5 DEG C / h, preserving heat and carrying out ultrasonic treatment. The method provided by the invention realizes efficient production and accurate purification of isomaltulose.

The invention provides a light diesel engine tail gas after-treatment system and a control method thereof, which can solve the problems of high cold start emission, catalyst poisoning caused by HC, N2O generation and difficulty in reaching the standard of PN10 emission in the prior art, meet the requirements of a new generation of emission regulations, and improve the emission efficiency. Comprising a ccDOC unit, a first urea injection unit, a ccSCR unit, a DPF unit, a second urea injection unit, a second SCR unit and an ASC unit which are sequentially arranged in the tail gas direction of an engine. The controller is electrically connected with the first urea spraying unit and the second urea spraying unit; and the controller controls the first urea injection unit to inject a first urea amount U1 into the tail gas in front of the ccSCR unit and controls the second urea injection unit to inject a second urea amount U2 into the tail gas in front of the second SCR unit according to a preset control method.