The invention discloses a 
trichoderma viride W2 capable of producing a thermophilic 
ethanol-resistant beta-
glucosidase and an application thereof. The 
trichoderma viride W2 is preserved in the China General 
Microbiological Culture Collection Center (CGMCC) on August 23, 2010, and the preservation number of the 
trichoderma viride W2 is CGMCC No.4098. The 
trichoderma viride W2 can produce a new beta-
glucosidase, the enzymatic activity of the new beta-
glucosidase reaches 346.7U / mL, the optimal reaction pH value is 4.8, the optimal 
reaction temperature is 70 DEG C, and the new beta-glucosidase is suitable for pyrohydrolysis and has an obvious glucose 
feedback inhibition effect. The 
ethanol the concentration of which is 10% has a maximal effect on promoting the enzymatic activity and improves the enzymatic activity of the beta-glucosidase by 1.6 times, and the resistant ability of the 
ethanol reaches 30%, so that the 
cellobiose inhibition can be effectively eliminated, the yield of the ethanol is improved by nearly 3 times, and the terminal 
product inhibition is effectively eliminated. Thus, the beta-glucosidase can be used for simultaneous saccharification and 
fermentation of lignocellulose raw materials, has a rare promoting effect in China, effectively increases the yield of the 
cellulosic ethanol, lowers the production cost, and accelerates the industrialized progress of the 
cellulosic ethanol.